CN102177809B - Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast - Google Patents
Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast Download PDFInfo
- Publication number
- CN102177809B CN102177809B CN 201010580172 CN201010580172A CN102177809B CN 102177809 B CN102177809 B CN 102177809B CN 201010580172 CN201010580172 CN 201010580172 CN 201010580172 A CN201010580172 A CN 201010580172A CN 102177809 B CN102177809 B CN 102177809B
- Authority
- CN
- China
- Prior art keywords
- selenium
- cultivation
- particle
- feed additives
- mushroom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000011669 selenium Substances 0.000 title claims abstract description 191
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 171
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 166
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000222350 Pleurotus Species 0.000 title abstract description 6
- 239000002245 particle Substances 0.000 claims abstract description 101
- 239000000654 additive Substances 0.000 claims abstract description 51
- 230000000996 additive effect Effects 0.000 claims abstract description 51
- 240000008042 Zea mays Species 0.000 claims abstract description 19
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 19
- 235000012054 meals Nutrition 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 19
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 18
- 235000009973 maize Nutrition 0.000 claims abstract description 18
- 238000012364 cultivation method Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 83
- 239000003674 animal food additive Substances 0.000 claims description 30
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 28
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 27
- 235000013339 cereals Nutrition 0.000 claims description 27
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 13
- 238000009826 distribution Methods 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 238000005469 granulation Methods 0.000 claims description 8
- 230000003179 granulation Effects 0.000 claims description 8
- 235000012255 calcium oxide Nutrition 0.000 claims description 7
- 239000000292 calcium oxide Substances 0.000 claims description 7
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 7
- 239000002361 compost Substances 0.000 claims description 7
- 239000003337 fertilizer Substances 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 39
- 239000000203 mixture Substances 0.000 abstract description 8
- 229940091258 selenium supplement Drugs 0.000 description 150
- 235000013399 edible fruits Nutrition 0.000 description 13
- 230000012010 growth Effects 0.000 description 12
- 150000001875 compounds Chemical class 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 10
- 235000013372 meat Nutrition 0.000 description 6
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 6
- 235000013599 spices Nutrition 0.000 description 6
- 230000002354 daily effect Effects 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000237502 Ostreidae Species 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000007726 management method Methods 0.000 description 4
- 235000020636 oyster Nutrition 0.000 description 4
- 241000282412 Homo Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000004567 concrete Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- -1 selenium polysaccharide Chemical class 0.000 description 1
- 229960002718 selenomethionine Drugs 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast, which is characterized by comprising steps of mixing a selenium-enriched cultivation material additive of which the weight is 1-10 percent of the total weight of a cultivation drier into the cultivation drier and carrying out cultivation according to a common cultivation method to obtain the uniform selenium-enriched pleurotus geesteranus, wherein the total content of selenium in the selenium-enriched cultivation material additive is in the range of 5-200mg/kg; the diameters of particles in the selenium-enriched cultivation material additive are distributed in a multiple diameter region mode in the range of 0.005-5.0mm; the weight of the particles with the diameter in the range of 1.0-5.0mm is at least 10 percent of the total weight of the additive; the weight of the particles with the diameter in the range of 0.005-0.1mm is at least 40 percent of the total weight of the additive; and the selenium-enriched cultivation material additive comprises the following composition raw materials in parts by weight: 100 parts of maize cob meal and 0.45-18 parts of selenium yeast. The pleurotus geesteranus obtained by using the method has high selenium utilization rate and stable selenium content between batches. Moreover, the method has no hidden danger of selenium pollution and is convenient to operate and easy to popularize.
Description
Technical field
The present invention relates to edible mushroom, particularly a kind of selenium nutrition-intensified method of elegant precious mushroom of carrying out take selenium yeast as selenium source belongs to the conversion technology field of selenium resource.
Background technology
Elegant precious mushroom (Pleurotus geesteranus) belongs to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae Pleurotus at taxonomy.Elegant precious mushroom quality is tender and crisp, fresh and sweet tasty and refreshing, has happy crab fragrance, and is nutritious, has higher edible and medical value, is the strain excellent in the oyster cap fungus.It is a kind of high protein, low-fat nutraceutical, its protein content, and close to meat, than the high 3-6 of general vegetables doubly, and its quality is delicate, and fiber content is few; More valuablely be, it contains ethnic group self and can not make, and the threonine that usually lacks in the food, lysine, homo amino acid etc.This kind edible mushroom is gained the name because of delicate and precious, and its shape is small and exquisite, and is delicious in taste, very popular, and in very great demand in the international market, DEVELOPMENT PROSPECT is wide.
Selenium is the necessary trace element of a kind of humans and animals, have remove free radical anti-oxidant (, delay senility, anticancer, strengthen the biological propertyes such as immunity, antagonism heavy metal.It is 50～250 μ g that a day selenium intake is recommended by Chinese Soclety of Nutrition.Human body selenium source is main to be taken in by meals, but China adult's average day selenium intake only have about 36 μ g, and therefore, the bad situation of selemium nutrition is at China's ubiquity.The data that WHO announces shows that also the whole world has more than 40 country to belong to low selenium or scarce selenium area.Selenium mainly is divided into inorganic selenium and Organic Selenium two large classes: the former comprises selenate, selenite etc., and inorganic selenium assimilation effect for humans and animals is poor, and risk of toxicity is large, and improper use very easily causes selenosis; The latter mainly comprises the forms such as seleno-amino acids, seleno albumen, selenium polysaccharide, good absorbing effect concerning humans and animals, and toxicity is little, is more suitable for mending the requirement of selenium.
Rich selenium agricultural byproducts are human body Selenium Supplement element effective ways, and therefore, technique of enriched selenium is the hot fields of scientists study exploitation.The nutritionist points out that except dietary fiber, edible mushroom contains the complete nutrients matter of needed by human body, and an adult eats the 100g edible mushroom every day, can obtain the 15-17g high-quality protein; Can obtain 600mg calcium, be equivalent to 80% of daily requirement; Can satisfy 11% of human body amphemera requirement, but Se content is lower than 5g/kg in the general edible mushroom.Existing edible mushroom selenium-enriching cultivation technology is mainly directly adds the selenium salting liquid in planting material, the method is simple and easy to do, cost is lower, but inorganic residual quantity is large, toxicity large, absorption difference, and edible mushroom is low to se use efficiency in the planting material, and major part rests on laboratory or small test stage; And whether the fruit body selenium content is stable in whole growth cycle, and namely can the Se content of different batches fruit body keep stablizing on the knees of the gods.
Summary of the invention
The problems such as the object of the invention is to provide the even selenium-enriching cultivation method of the precious mushroom of a kind of show, and it is batch inhomogeneous to have solved in the prior art behind the selenium-enriching cultivation after the growth of elegant precious mushroom head tide or the many tide growths between elegant each strain of precious mushroom Se content, and the rich selenium amount fluctuation of fruit body is large.
In order to solve these problems of the prior art, technical scheme provided by the invention is:
A kind of method of utilizing selenium yeast to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight～10%, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5～200mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is many particle size region and distributes in the selenium-enriching cultivation feed additives in 0.005～5.0mm scope, and particle diameter is that the particle of 1.0～5.0mm accounts for 10% of additive gross weight at least; Particle diameter is that the particle of 0.005～0.1mm accounts for 40% of additive gross weight at least; The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Selenium yeast 0.45～18 parts by weight.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10～20% of 1.0～5.0mm;
Particle diameter is the particle 40～70% of 0.005～0.1mm;
The particle 20～40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 30% of other particle size range.
Preferably, described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
Preferably, described common cultivation method comprises that the cultivation siccative that will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio, abundant stirring and evenly mixing, heap fermentation at 1: 1.3, use the steam sterilization sterilization treatment after the pack, make the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
Preferably, the maize cob meal that uses of described selenium-enriching cultivation feed additives is pulverized as natural crops offcuts corncob and is formed.
Preferably, the particle of described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the pulverizing of selenium yeast raw material.
Preferably, when being used for the cultivation of health care medicinal food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 100～200mg/kg; When being used for daily edible selenium enriched oyster mushroom cultivation, the Se content of the selenium-enriching cultivation feed additives of use is 5～50mg/kg.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 60% of 0.005～0.1mm;
The particle 30% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 70% of 0.005～0.1mm;
The particle 20% of other particle size range.
Wherein A～B% represents scope in A% arrives the B% scope, and comprises B% and A%.
The inventor is through a large amount of research and test, combine the many tide growths of elegant precious mushroom and the different characteristics of each tide growth amount of gathering, by preparing a kind of different proportioning features of different grain size that have, discharge the specialist additive of selenium wherein with different speed, a kind of selenium nutrition-intensified method of elegant precious mushroom of carrying out take selenium yeast as selenium source is provided, realizes the stability of rich selenium amount between each damp mushroom and to the high usage of selenium resource and the high conversion ratio that absorbs.
The inventive method se use efficiency is high, without selenium contamination hidden danger, handled easily, be easy to promote and can realize safe and reliable in the product, stablize controlledly, realize that elegant precious mushroom is selenium nutrition-intensified.Below be concrete operation step of the present invention:
At first prepare a kind of selenium enriched oyster mushroom planting material additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, designs the prescription of the required planting material additive of the precious mushroom of cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals
0.45～18 part of selenium yeast;
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; With itself and the abundant stirring and evenly mixing of maize cob meal, make particle diameter below 0.1mm, selenium content is compound in 5～500mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of particle diameters are respectively: the particle of 0.005～0.1mm, 0.1～1.0mm, 1.0～5.0mm mixes by following percentage by weight: 40～70%, 20～40%, 10～20%, namely obtain having the selenium-enriching cultivation feed additives of different grain size proportioning feature, its total selenium content is still in the 5-200mg/kg scope.
According to the difference of required selenium enriched oyster mushroom product purpose, select the different planting material additive of selenium content during use.For example, the selenium enriched oyster mushroom planting material additive in daily edible rich selenium mushroom planting and selecting 5～50mg/kg scope; Health care medicinal is eaten the planting material additive in rich selenium mushroom planting and selecting 100～200mg/kg scope.
The basis planting material forms: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.During the planting material spice, the ratio in 1～10% is sneaked into basic planting material with additive, and stirring and evenly mixing makes in the planting material final selenium content in 0.5～20mg/kg scope.
Add running water by material-water ratio 1: 1.3 and fully stir, mixing again, heap fermentation is used the steam sterilization sterilization treatment after the pack, makes final composts or fertilisers of cultivating inoculated and cultured selenium enriched oyster mushroom; Also can directly adopt the cultivation in raw material mode to produce the selenium enriched oyster mushroom.
When the precious massee fruiting bodies of show was gathered, sampling detected wherein Se content, and bright sample Se content improves 6～240 times than the precious mushroom of common cultivation show, can reach 0.2～8.5mg/kg.And organic selenium content significantly increases in the fruit body, and the selenium that wherein exists with various seleno-amino acids forms accounts for total selenium more than 85%.
Statistics output and se use efficiency in the damp mushroom recovery process, the result shows: selenium nutrition-intensified group than common cultivation group head tide mushroom output increased 20%, and se use efficiency is up to 40%.
In the whole growth cycle of fruit body, the relative standard deviation RSD% of its selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD% is higher than 30%) edible mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 5%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 minutes than the precious mushroom of common show.
Utilize the present invention can obtain rich selenium better effects if, organic selenium content is higher, the elegant precious mushroom that rich selenium quality is more stable, and promoting elegant precious mushroom product is the high-quality Selenium-enriched health food, is that daily delicacies can be used as again the dietotherapy good merchantable brand, the people that are more convenient for accept.
With respect to scheme of the prior art, advantage of the present invention is:
(1) the planting material additive for preparing among the present invention is the selenium-enriched edible mushroom specialist additive, combine the edible fungi growth characteristics, have different grain size proportioning feature, can realize that selenium wherein discharges with different speed, thereby realize the stability of rich selenium amount between each damp mushroom.
(2) yeast selenium itself just has selenium form safety, characteristics that bioavailability is high, more utilizes elegant precious mushroom that it is absorbed; Maize cob meal can prevent effectively that as a kind of selenium source carrier selenium source runs off and the even distribution of assurance selenium source.
(3) the present invention realizes the stability of additive quality by steps such as elementary compound preparation, granulation and each granularity particle mix in varing proportions; Realize again mixing by spice before the planting material fermentation, this just guarantees the even distribution of selenium resource in planting material, is beneficial to mycelium and fruit body to the even absorption of selenium.
(4) by additive and common cultivation material by a certain percentage (1～10%) mix, make the planting material of final selenium content in 0.5～20mg/kg scope, can make that the selenium resource is absorbed and used better in the planting material, organic selenium content significantly improves in the fruit body.Testing result shows that selenomethionine is the main occurrence patterns of selenium in the elegant precious mushroom, and this form selenium accounts for 85～89% of total Se content.
(5) use the selenium enriched oyster mushroom that the present invention produces, bright mushroom selenium content can be strict controlled between 0.2～2.5mg/kg and the 3～8.5mg/kg, and the former can be for daily edible, and the latter can be used as food therapeutic health.
(6) statistics output and se use efficiency in the damp mushroom recovery process, the result shows: selenium nutrition-intensified group than common cultivation group head tide mushroom output increased more than 20%; Se use efficiency is higher than the selenium enriched oyster mushroom that utilizes the cultivation of inorganic selenium salt under the equal conditions far away up to 40%.
(7) in the whole growth cycle of fruit body, the relative standard deviation RSD of its selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) edible mushroom of the additive cultivation of different grain size proportioning feature.
(8) additive rich in selenium of the present invention's preparation is suitable for the elegant precious mushroom cultivation take any material as the cultivation base-material, such as sawmilling end, wheat bran, corn stalk, corncob, rape straw, cotton seed hulls etc.
Embodiment
Below in conjunction with specific embodiment such scheme is described further.Should be understood that these embodiment are not limited to limit the scope of the invention for explanation the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and not marked implementation condition is generally the condition in the normal experiment.
Embodiment 1 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required planting material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.45～0.9 part of selenium yeast.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; After it is crushed to particle diameter and is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, namely make particle diameter below 0.1mm, selenium content is compound in 5～10mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. the particle with above-mentioned three kinds of particle diameters mixes by following percentage by weight: 70%, 20%, 10%, namely obtain having different grain size proportioning feature, and selenium content is the selenium-enriching cultivation feed additives of 5～10mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select the additive such as preparation as described in (1); The common cultivation material forms: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the planting material spice, the ratio in 10% and common cultivation material mixing, making final selenium content is 0.5～1.0mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing again, heap fermentation use the steam sterilization sterilization treatment after the pack, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to the precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom output is than common cultivation group output increased 20%, and se use efficiency is up to 40%; Total Se content is about 0.2～0.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 6～15 times of common cultivation group, and the selenium that wherein exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 minutes than the precious mushroom of common show.
Embodiment 2 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required planting material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.9～4.5 part of selenium yeast.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; After it is crushed to particle diameter and is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, namely make particle diameter below 0.1mm, selenium content is compound in 10～50mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. the particle with above-mentioned three kinds of particle diameters mixes by following percentage by weight: 60%, 30%, 10%, namely obtain having different grain size proportioning feature, and selenium content is the selenium-enriching cultivation feed additives of 10～50mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select the additive such as preparation as described in (1); The common cultivation material forms: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the planting material spice, the ratio in 10% and common cultivation material mixing, making final selenium content is 1～5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing again, heap fermentation use the steam sterilization sterilization treatment after the pack, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to the precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom output is than common cultivation group output increased 20%, and se use efficiency is up to 40%; Total Se content is 0.5～2.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 15～70 times of common cultivation group, and the selenium that wherein exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 minutes than the precious mushroom of common show.
Embodiment 3 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required planting material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
4.5～9 parts of selenium yeasts.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; After it is crushed to particle diameter and is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, namely make particle diameter below 0.1mm, selenium content is compound in 50～100mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. the particle with above-mentioned three kinds of particle diameters mixes by following percentage by weight: 60%, 20%, 20%, namely obtain having different grain size proportioning feature, and selenium content is the selenium-enriching cultivation feed additives of 50～100mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select the additive such as preparation as described in (1); The common cultivation material forms: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the planting material spice, the ratio in 5% and common cultivation material mixing, making final selenium content is 2.5～5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing again, heap fermentation use the steam sterilization sterilization treatment after the pack, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to the precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom output is than common cultivation group output increased 20%, and se use efficiency is up to 40%; Total Se content is about 1.0～2.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 25～70 times of common cultivation group, and the selenium that wherein exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 minutes than the precious mushroom of common show.
Embodiment 4 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required planting material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
9～18 parts of selenium yeasts.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; After it is crushed to particle diameter and is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, namely make particle diameter below 0.1mm, selenium content is compound in 100～200mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. the particle with above-mentioned three kinds of particle diameters mixes by following percentage by weight: 50%, 40%, 10%, namely obtain having different grain size proportioning feature, and total selenium content is the selenium-enriching cultivation feed additives of 100～200mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select the additive such as preparation as described in (1); The common cultivation material forms: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the planting material spice, the ratio in 10% and common cultivation material mixing, making final selenium content is 10～20mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing again, heap fermentation use the steam sterilization sterilization treatment after the pack, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to the precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom output is than common cultivation group output increased 20%, and se use efficiency is up to 40%; Total Se content is 3～8.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 85～240 times of common cultivation group, and the selenium that wherein exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 minutes than the precious mushroom of common show.
Above-mentioned example only is explanation technical conceive of the present invention and characteristics, and its purpose is to allow the people who is familiar with technique can understand content of the present invention and according to this enforcement, can not limit protection scope of the present invention with this.All equivalent transformations that Spirit Essence is done according to the present invention or modification all should be encompassed within protection scope of the present invention.
Claims (8)
1. method of utilizing selenium yeast to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight 1～10%, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5～200mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is three particle size region and distributes in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10～20% of 1.0～5.0m m;
Particle diameter is the particle 40～70% of 0.005～0.1m m;
The particle 20～40% of other particle size range;
The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Selenium yeast 0.45～18 parts by weight.
2. method according to claim 1 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0m m scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0m m;
Particle diameter is the particle 50% of 0.005～0.1m m;
The particle 40% of other particle size range.
3. method according to claim 1 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0m m scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0～5.0m m;
Particle diameter is the particle 50% of 0.005～0.1m m;
The particle 30% of other particle size range.
4. method according to claim 1 is characterized in that described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
5. method according to claim 1, it is characterized in that described common cultivation method comprises that the cultivation siccative of will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio at 1: 1.3, abundant stirring and evenly mixing, heap fermentation, use the steam sterilization sterilization treatment after the pack, make the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
6. method according to claim 1 is characterized in that the maize cob meal that described selenium-enriching cultivation feed additives uses forms as the pulverizing of natural crops offcuts corncob.
7. method according to claim 1 is characterized in that the particle of described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the pulverizing of selenium yeast raw material.
8. method according to claim 1, when it is characterized in that for the cultivation of health care medicinal food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 100～200mg/kg; When being used for daily edible selenium enriched oyster mushroom cultivation, the Se content of the selenium-enriching cultivation feed additives of use is 5～50mg/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010580172 CN102177809B (en) | 2010-12-09 | 2010-12-09 | Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010580172 CN102177809B (en) | 2010-12-09 | 2010-12-09 | Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102177809A CN102177809A (en) | 2011-09-14 |
| CN102177809B true CN102177809B (en) | 2013-04-03 |
Family
ID=44564226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010580172 Active CN102177809B (en) | 2010-12-09 | 2010-12-09 | Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102177809B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107371786A (en) * | 2017-06-15 | 2017-11-24 | 柳城新天地生态农业发展有限公司 | The method for improving Se content in elegant precious mushroom |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511305A (en) * | 2011-12-07 | 2012-06-27 | 苏州硒谷科技有限公司 | Method for cultivating high-selenium-tolerability strain of edible fungi |
| CN102515884B (en) * | 2011-12-07 | 2014-01-08 | 苏州硒谷科技有限公司 | Edible fungi organic selenium additive, preparation method and application thereof |
| CN102630480B (en) * | 2012-04-01 | 2013-11-06 | 苏州安德鲁尹生物技术咨询有限公司 | Iron-rich black fungus and production method thereof |
| CN103039268A (en) * | 2012-12-11 | 2013-04-17 | 镇江市丹徒区南山溪园茶叶专业合作社 | Method for producing selenium-rich and zinc-rich Pleurotus geesteranus |
| CN103524173B (en) * | 2013-10-08 | 2015-04-15 | 开原市御隆粮米加工厂 | Agricultural tablet for improving rice nutrition |
| CN104521550A (en) * | 2014-12-01 | 2015-04-22 | 云南金雨庄生物科技有限责任公司 | Selenium-rich grifola frondosa cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182266A (en) * | 2007-10-31 | 2008-05-21 | 杭州农发科技开发有限公司 | Method for planting selenium-enriched pleurotus eryngii and culture medium thereof |
| CN101496484A (en) * | 2008-01-31 | 2009-08-05 | 马俊生 | Method for domesticating and cultivating selenium-rich mushroom strain |
| CN101844939A (en) * | 2009-03-26 | 2010-09-29 | 苏州硒谷科技有限公司 | Edible fungi culture medium additive, preparation and application thereof |
-
2010
- 2010-12-09 CN CN 201010580172 patent/CN102177809B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182266A (en) * | 2007-10-31 | 2008-05-21 | 杭州农发科技开发有限公司 | Method for planting selenium-enriched pleurotus eryngii and culture medium thereof |
| CN101496484A (en) * | 2008-01-31 | 2009-08-05 | 马俊生 | Method for domesticating and cultivating selenium-rich mushroom strain |
| CN101844939A (en) * | 2009-03-26 | 2010-09-29 | 苏州硒谷科技有限公司 | Edible fungi culture medium additive, preparation and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 杜昌文等.聚合物包膜肥料中钾素释放特征及其模拟.《农业工程学报》.2006,第22卷(第02期),第19页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107371786A (en) * | 2017-06-15 | 2017-11-24 | 柳城新天地生态农业发展有限公司 | The method for improving Se content in elegant precious mushroom |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102177809A (en) | 2011-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150555B (en) | Method for culturing pleurotus geesteranus uniformly rich in selenium by using mixed selenium source | |
| CN102150557B (en) | Method for culturing pleurotus geesteranus uniformly rich in selenium by using selenium-enriched crop stalks | |
| CN103449901B (en) | A kind of cultivating chicken leg mushroom base-material and preparation method thereof | |
| CN102177809B (en) | Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast | |
| CN107098784A (en) | Biological organic fertilizer and preparation method thereof | |
| CN101156659A (en) | Organic swine core premix | |
| CN102771313A (en) | Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws | |
| CN102165894B (en) | Method for carrying out rich-selenium culturing of plelmtus eqngiu by utilizing sodium selenite | |
| CN103467169A (en) | Black fungus cultivation base material and preparation method thereof | |
| CN102150559B (en) | Method for cultivating oyster mushroom uniformly rich in selenium with sodium selenite as selenium source | |
| CN102150558B (en) | Method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate | |
| CN102763562A (en) | Cultivating method for high-selenium edible fungus | |
| CN104311309A (en) | Indoor practical straw mushroom culture medium and preparation method thereof | |
| CN105777403A (en) | Special selenium-rich organic fertilizer for navel oranges in red soil region | |
| CN102150556A (en) | Method for cultivating pleurotus eryngii uniformly rich in selenium with mixed organic selenium source | |
| CN102763561A (en) | Cultivation method of high-selenium coprinus comatus | |
| CN106007844A (en) | Special selenium-rich bio-organic fertilizer for broccoli as well as preparation method and application of fertilizer | |
| CN106720973A (en) | A kind of organic standard pig premix and preparation method thereof | |
| CN103214305A (en) | Method for inoculating liquid strains and cultivating edible fungi by adopting straws as main materials | |
| CN102038105A (en) | Method for producing harmless selenium-rich black earth pork by utilizing selenium-rich astragalus sinicus secondary conversion technology | |
| CN104151033A (en) | Ganoderma lucidum culture medium containing beet pulp and preparation method thereof | |
| CN102018134A (en) | Black carp feed and preparation method thereof | |
| CN102432375A (en) | Mineral organic fertilizer for watermelons and production method thereof | |
| CN104072298A (en) | High-safety high-efficiency environment-friendly biological fish fertilizer and preparation method thereof | |
| CN107500913A (en) | A kind of culture medium of cultivating champignon and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20110914 Assignee: Anhui Setek Biological Technology Co., Ltd. Assignor: Suzhou Se Technology Co., Ltd. Contract record no.: 2013320010174 Denomination of invention: Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast Granted publication date: 20130403 License type: Exclusive License Record date: 20131224 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210204 Address after: 239001 room 207, 2nd floor, building 1, NO.666, Hongwu Road, Wuyi Town, Chuzhou City, Anhui Province Patentee after: ANHUI SELENIUM VALLEY BIOTECH Co.,Ltd. Address before: 215123 executive building, 166 Jen Ai Road, Suzhou Industrial Park, Jiangsu Patentee before: SUZHOU SETEK Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210315 Address after: No.238 Huaiyuan South Street, Shapotou District, Zhongwei City, Ningxia Hui Autonomous Region Patentee after: Ningxia selenium Industry Development Co.,Ltd. Address before: 239001 room 207, 2nd floor, building 1, NO.666, Hongwu Road, Wuyi Town, Chuzhou City, Anhui Province Patentee before: ANHUI SELENIUM VALLEY BIOTECH Co.,Ltd. |