CN102133231B - Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof - Google Patents
Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof Download PDFInfo
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- CN102133231B CN102133231B CN2011100701690A CN201110070169A CN102133231B CN 102133231 B CN102133231 B CN 102133231B CN 2011100701690 A CN2011100701690 A CN 2011100701690A CN 201110070169 A CN201110070169 A CN 201110070169A CN 102133231 B CN102133231 B CN 102133231B
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Abstract
The invention discloses a product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage, comprising the following matters according to part by weight, 20-80 parts of shark cartilage organic calcium, 1-20 parts of collagen and 1-20 parts of mucopolysaccharide. The invention also discloses a preparation method of the product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage. The preparation method of the shark cartilage organic calcium comprises the following steps: curing, acidizing, enzymolysis, deproteinization, crystallization, resin pretreatment, resin exchange, ultra-filtration dehydration, filtrate calcification, dehydration and drying. The preparation method of the collagen and the mucopolysaccharide comprises the following steps: boiling the shark cartilage by water, then crushing and conducting homogenate on the shark cartilage, removing sediments by filtering, adding filtrate with macroporous resin for absorption, then conducting elution by sodium chloride solution, and finally adding ethanol in eluent for settlement. The product has the beneficial effects that calcium is supplemented, the cartilage metabolism is promoted, the cartilage development of children is enhanced, the cartilage injure is repaired, the absorption rate of a human body is high, and the side effect does not exist.
Description
Technical field
The invention belongs to health product and preparation method thereof, relate to product and method for making thereof that a kind of shark cartilage preparation is replenished the calcium and promoted the cartilage metabolism growth.
Background technology
Show according to " Chinese residents nutrition is with healthy " investigation report in 2002, Chinese's calcium deficiency situation is still very serious, and the daily intaking amount of resident's calcium is 391 milligrams, only is equivalent to 41% of recommended intake.Oxalic acid in the vegetable, dietary fiber also can stop calcareous absorption.The dietary habit of Chinese residents directly causes the people of China 90% to be in throughout one's life the calcifame statural state.Existing market has emerged a large amount of calcium supplementing products, but most products exist calcium content low, absorbs the problems such as very difficult.Shark cartilage calcium has multiple pharmacological effect and biological function as the important component part of connective tissue, often is used as dietary supplement and uses, and is mainly used in osteoarthritis and promotes cartilage development and the treatment of cartilage injury's reparation.The traditional method that the shark cartilage organic calcium is extracted mainly contains alkali and puies forward enzymatic isolation method, alkaline extraction, ultrasound wave auxiliary law and acetic acid extraction method, and common alkaline process production technology exists also that purity is low, the ethanol use amount causes greatly the high defective of production cost.
Summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, and replenishing the calcium and promoting product and the method for making thereof that cartilage metabolism is grown, this product can replenish the calcium and promote cartilage metabolism, strengthen child's cartilage development, repair the cartilage injury of a kind of shark cartilage preparation is provided.
The present invention is achieved through the following technical solutions:
The replenishing the calcium and promote the product that cartilage metabolism is grown of shark cartilage of the present invention preparation, its special character is: the material that comprises following parts by weight: shark cartilage organic calcium 20-80 part, collagen protein 1-20 part and each 1-20 part of mucopolysaccharide.
The replenishing the calcium and promote the product that cartilage metabolism is grown of shark cartilage of the present invention preparation, the material that also comprises following parts by weight: calcareous short receipts agent phosphopeptide caseinate CPP 0.5-1.5 part, immune factor AKGs AKGS 0.0005-0.001 part, vitamin D 0.000002-0.000005 part, essence 0.1-0.3 part.
Dosage form is tablet or hard capsule, perhaps adds entry and glycerol is made liquid capsule.
The replenishing the calcium and promote the method for making of the product that cartilage metabolism is grown of shark cartilage of the present invention preparation, its special character is:
The method for making of shark cartilage organic calcium is made of successively following steps: ripening, acidify, enzymolysis, Deproteinization, crystallization, resin pretreatment, resins exchange, ultrafiltration, filtrate calcification, dehydrate;
The method for making of collagen protein and mucopolysaccharide is made of successively following steps: pulverize homogenate behind the shark cartilage decocting in water, remove by filter precipitation, filtrate adds macroporous resin adsorption, then uses the sodium chloride solution eluting, eluent adds the ethanol precipitation, is shark cartilage collagen albumen and mucopolysaccharide;
With shark cartilage organic calcium, collagen protein and mucopolysaccharide, perhaps add calcareous short receipts agent phosphopeptide caseinate CPP at last, immune factor AKGs AKGS, vitamin D and natural essence are prepared into required dosage form.
Acidification step in the method for making of shark cartilage organic calcium: add lactic acid after adopting refiner homogenate, be heated to 30-40 ℃ of stirring and be acidified to chondrolysis.
The invention has the beneficial effects as follows, the present invention not only can improve the purity of shark cartilage organic calcium, can improve calcium content simultaneously, also can save the use amount of ethanol, reduces production costs.The replenishing the calcium and promote the product that cartilage metabolism is grown of shark cartilage preparation, can replenish the calcium and can under the effect of collagen protein and mucopolysaccharide, promote cartilage metabolism, strengthen child's cartilage development, repair the cartilage injury, not only satisfied the problem that dyschondroplasia that replenishing the calcium of people required but also solved a large amount of children and cartilage injury repair, the human absorptivity is high and have no side effect, the absorbance of existing calcium supplementing product only is 10-50%, and this product absorbance is 90-98%, and calcium can be deposited in the skeleton better.Because this product contains immune factor AKGs AKGS, improve the immunity of people's body in addition, can satisfy all kinds of crowds' diversified needs.Method for making of the present invention not only can be extracted organic calcium, collagen protein and the mucopolysaccharide in the shark cartilage, and can extract other Fish, domestic animal, the endochondral organic calcium of poultry, collagen protein and mucopolysaccharide, and is applied widely.
The specific embodiment
Embodiment 1
The replenishing the calcium and promote the product that cartilage metabolism is grown of shark cartilage of the present invention preparation, the material that comprises following weight: shark cartilage organic calcium 50 grams, each 15 gram of collagen protein and mucopolysaccharide, calcareous short receipts agent phosphopeptide caseinate CPP 1 gram, immune factor AKGs AKGS 0.0008 gram, vitamin D 0.000004 gram, natural essence 0.2 gram, to adopt the technique of liquid type to be prepared into liquid capsule behind its mix homogeneously, every capsules contains shark cartilage organic calcium 0.3 gram.Natural essence is fruity essence.
Usage and dosage: once a day, at every turn use the oral 1-6 grain of warm water delivery service.The child is 1 below 2 years old, 2 of 2-12 years.Adult 3-6 grain increases and decreases according to state of an illness weight in this scope.
The replenishing the calcium and promote the method for making of the product that cartilage metabolism is grown of shark cartilage of the present invention preparation:
The method for making of shark cartilage organic calcium is made of successively following steps: ripening, acidify, enzymolysis, Deproteinization, crystallization, resin pretreatment, resins exchange, ultrafiltration, filtrate calcification, dehydrate;
The method for making of collagen protein and mucopolysaccharide is made of successively following steps: pulverize homogenate behind the shark cartilage decocting in water, remove by filter precipitation, filtrate adds macroporous resin adsorption, then uses the sodium chloride solution eluting, eluent adds the ethanol precipitation, is shark cartilage collagen albumen and mucopolysaccharide;
With shark cartilage organic calcium, collagen protein and mucopolysaccharide, perhaps add calcareous short receipts agent phosphopeptide caseinate CPP at last, immune factor AKGs AKGS, vitamin D and natural essence are prepared into required dosage form.
The present invention extracts high-purity cartilage organic calcium and collagen protein, mucopolysaccharide from shark cartilage.The preparation detailed step of cartilage organic calcium is as follows:
Ripening: get shark cartilage, under neutrallty condition, 80-90 ℃ decocting in water 1-3 hour, wash down and remove oils and fats and add again water boil, be incubated 2-5 hour;
Acidify: after adopting refiner homogenate, the 3-10% that presses the shark cartilage inventory adds lactic acid, is heated to 30-40 ℃, stirs acidify 1-5 hour, stopping acidification after chondrolysis;
Enzymolysis: be 8-10 with the sodium hydroxide adjust pH, temperature 40-60 ℃, add pancreatin by the 0.1-0.4% of cartilage inventory and carry out enzyme digestion reaction;
Deproteinization: adjusting pH value with hydrochloric acid is 7-9, is warming up to 80-100 ℃, is incubated 1-3 hour, filters, and obtains settled solution;
Crystallization: add 95% ethanol in settled solution, make concentration of alcohol reach 70%, crystallization is filtered and is obtained shark cartilage organic calcium semifinished product;
Resin pretreatment: get solubility shark cartilage organic calcium and be dissolved in water, with 732 strongly acidic styrene type cation exchange resin separation and purification, use water rinse, soak, with the alkali cleaning of 1mol/L sodium hydroxide, use the 1mol/L chlorohydric acid pickling, water washes the 6-7 to pH again, by wet method dress post;
Resins exchange: get step solubility shark cartilage organic calcium, be dissolved in water by weight, make the solution of 5%-15%, upper prop is controlled the rate of outflow well, and with the pH of acidometer on-line monitoring effluent, when the pH of effluent becomes 4 by neutrality, begin to collect effluent, when pH becomes 4 by strong acid, stop to collect;
Ultrafiltration: get above-mentioned ion exchanged soln, ultrafiltration is sloughed molecular weight simultaneously less than impurity such as 1000 polypeptide, aminoacid;
The filtrate calcification: get the concentrated solution behind the ultrafiltration, regulate pH to 3-8 with sodium hydroxide water saturation liquid, add calcium chloride again, stirring and dissolving is filtered, and the filtering residue dehydrate obtains shark cartilage organic calcium highly finished product.
The preparation detailed step of collagen protein and mucopolysaccharide sequentially comprises: cartilage adds the water of 10 times of weight, homogenate, and homogenate is heated to 90 ℃ and keeps 2h, is cooled to room temperature, filters and removes precipitation.Filtrate adds 20% the macroporous resin account for cartilage weight, adopts sodium hydroxide to transfer pH 9.0, and stirring and adsorbing 4h. filters and collects resin, resin water flushing 3 times, with 3 mol/L sodium chloride solution eluting, eluent adds 3 times of volume ethanol, filter collecting precipitation, precipitation is collagen and mucopolysaccharide.
With shark cartilage organic calcium, collagen protein and mucopolysaccharide, perhaps add calcareous short receipts agent phosphopeptide caseinate CPP at last, immune factor AKGs AKGS, vitamin D and natural essence are prepared into required dosage form.
Extract the method for cartilage organic calcium in the shark cartilage of the present invention owing to adopting weak acid to process, make the cartilage organic calcium in the protein free out, can effectively remove the impurity such as protein, improve purity and the content of shark cartilage organic calcium, adopt cation exchange resin, finish the sodium process with column chromatography, the shark cartilage organic calcium concentration of exchange is high, and can accurately control the shark cartilage organic calcium that generates in the ion exchange process, sodium content is low in the product, and the hyperfiltration process dehydration both can have been played concentrated, can slough simultaneously small molecular weight impurity again, improve the purity of shark cartilage organic calcium, can improve calcium content simultaneously.Also can save the use amount of ethanol, reduce production costs.
The preparation optimal case of shark cartilage organic calcium is: get shark cartilage 200g, add water 2000 grams, be heated to 90 ℃ of decocting in water 2 hours, wash down the removal oils and fats and added water boil 4 hours again; Add the lactic acid of 20 ml 80% after the homogenate of employing refiner, 40 ℃ are stirred acidify 5 hours, stopping acidification after chondrolysis; Be 8 with the sodium hydroxide adjust pH, be heated to 55 ℃ of temperature, add the 0.6g pancreatin, add at twice, add 0.4g for the first time, add 0.2g after three hours, constantly stir, enzyme digestion reaction 6 hours, adjusting pH value with hydrochloric acid is 7.5, be warming up to 80 ℃, be incubated 2 hours, filter, obtain settled solution, add 95% ethanol, make concentration of alcohol reach 70%, stirred once every 30 minutes, crystallization is filtered and is obtained shark cartilage organic calcium semifinished product, and 80g weighs.With 500ml hot water dissolving shark cartilage organic calcium semifinished product, with 732 strongly acidic styrene type cation exchange resin separation and purification, use water rinse, soak, with 1mol/L sodium hydroxide 500ml alkali cleaning, with 1mol/L hydrochloric acid 500ml pickling, water washes the 6-7 to pH again, by wet method dress post; With the pH of acidometer on-line monitoring effluent, when the pH of effluent becomes 4 by neutrality, begin to collect effluent, when pH becomes 4 by strong acid, stop to collect.Holding back greater than 1000 daltonian shark cartilage organic calcium through ultrafiltration, is 300ml after ultrafiltration is concentrated again.Regulate pH to 6 with sodium hydroxide water saturation liquid, add calcium chloride 140g again, stirring and dissolving is filtered.Precipitation is dewatered with dehydrated alcohol, and 65 ℃ of vacuum dryings get shark cartilage organic calcium 50g, and purity reaches 98% after testing, and all other indexs meet specification requirement.
The preparation optimal case of collagen protein and mucopolysaccharide is: get shark cartilage 200g, add water 2000 grams, be heated to 90 ℃ of decocting in water 2 hours, wash down the removal oils and fats and added water boil 4 hours again; Adopt refiner homogenate.Homogenate is transferred pH8-9 with sodium hydroxide, and 90 ℃ of decocting in water 2 hours are cooled to room temperature, filter and remove precipitation, filtrate adds 40 g macroporous adsorbent resins, is heated to 50 ℃ of stirring and adsorbing 4 hours, filter and collect resin, with clear water washing 3 times, adopt the each 500ml eluting of 3mol/L sodium chloride solution 2 times, eluent adds the dehydrated alcohol of 3 times of volumes, room temperature is placed and is spent the night, filter collecting precipitation, lyophilization is shark cartilage mucopolysaccharide and shark collagen protein.
Embodiment 2
The replenishing the calcium and promote the preparation of the product that cartilage metabolism is grown of shark cartilage preparation: according to prescription shark cartilage organic calcium 80 grams, collagen protein 20 grams, mucopolysaccharide 16 grams, immune factor AKGs AKGS 0.001 gram, calcareous short receipts agent phosphopeptide caseinate 1.5 grams, vitamin D 0.000005 gram, natural essence 0.3 gram will adopt the technique of liquid type to be prepared into the liquid capsule grain behind its mix homogeneously.
All the other are identical with embodiment 1.
Embodiment 3
The replenishing the calcium and promote the preparation of the product that cartilage metabolism is grown of shark cartilage preparation: be respectively 1 gram according to prescription shark cartilage organic calcium 20 grams, collagen protein and mucopolysaccharide, immune factor AKGs AKGS 0.0005 gram, calcareous short receipts agent phosphopeptide caseinate 0.5 gram, vitamin D 0.000002 gram, natural essence 0.1 gram will adopt the technique of liquid type to be prepared into the liquid capsule grain behind its mix homogeneously.
All the other are identical with embodiment 1.
Embodiment 4
The replenishing the calcium and promote the preparation of the product that cartilage metabolism is grown of shark cartilage preparation: according to prescription shark cartilage organic calcium 40 grams, shark cartilage collagen albumen and mucopolysaccharide are respectively 9 grams, to adopt the technique of solid type to be prepared into tablet behind its mix homogeneously, every contains shark cartilage organic calcium 0.3 gram.
Usage and dosage: once a day, at every turn use the oral 1-6 sheet of warm water delivery service.The child is 1 below 2 years old, 2 of 2-12 years.Adult 3-6 sheet increases and decreases according to state of an illness weight in this scope.
All the other are identical with embodiment 1.
Embodiment 5
The replenishing the calcium and promote the preparation of the product that cartilage metabolism is grown of shark cartilage preparation: according to prescription shark cartilage organic calcium 70 grams, shark cartilage collagen albumen 13 grams, mucopolysaccharide 20 grams will adopt the technique of solid type be prepared into hard capsule behind its mix homogeneously.Every capsules contains shark cartilage organic calcium 0.3 gram.
Usage and dosage: once a day, at every turn use the oral 1-6 grain of warm water delivery service.The child is 1 below 2 years old, 2 of 2-12 years.Adult 3-6 grain increases and decreases according to state of an illness weight in this scope.
All the other are identical with embodiment 1.
Claims (4)
1. a shark cartilage prepares the method for replenishing the calcium and promoting the product that cartilage metabolism is grown, and it is characterized in that:
The preparation process of shark cartilage organic calcium is as follows:
Ripening: get shark cartilage, under neutrallty condition, 80-90 ℃ decocting in water 1-3 hour, wash down and remove oils and fats and add again water boil, be incubated 2-5 hour;
Acidify: after adopting refiner homogenate, the 3-10% that presses the shark cartilage inventory adds lactic acid, is heated to 30-40 ℃, stirs acidify 1-5 hour, stopping acidification after chondrolysis;
Enzymolysis: be 8-10 with the sodium hydroxide adjust pH, temperature 40-60 ℃, add pancreatin by the 0.1-0.4% of cartilage inventory and carry out enzyme digestion reaction;
Deproteinization: adjusting pH value with hydrochloric acid is 7-9, is warming up to 80-100 ℃, is incubated 1-3 hour, filters, and obtains settled solution;
Crystallization: add 95% ethanol in settled solution, make concentration of alcohol reach 70%, crystallization is filtered and is obtained shark cartilage organic calcium semifinished product;
Resin pretreatment: get solubility shark cartilage organic calcium and be dissolved in water, with 732 strongly acidic styrene type cation exchange resin separation and purification, use water rinse, soak, with the alkali cleaning of 1mol/L sodium hydroxide, use the 1mol/L chlorohydric acid pickling, water washes the 6-7 to pH again, by wet method dress post;
Resins exchange: get step solubility shark cartilage organic calcium, be dissolved in water by weight, make the solution of 5%-15%, upper prop is controlled the rate of outflow well, and with the pH of acidometer on-line monitoring effluent, when the pH of effluent becomes 4 by neutrality, begin to collect effluent, when pH becomes 4 by strong acid, stop to collect;
Ultrafiltration: get above-mentioned ion exchanged soln, ultrafiltration is sloughed molecular weight simultaneously less than 1000 polypeptide, aminoacid impurity;
The filtrate calcification: get the concentrated solution behind the ultrafiltration, regulate pH to 3-8 with sodium hydroxide water saturation liquid, add calcium chloride again, stirring and dissolving is filtered, and the filtering residue dehydrate obtains shark cartilage organic calcium highly finished product;
The preparation process of collagen protein and mucopolysaccharide sequentially comprises: cartilage adds the water of 10 times of weight, homogenate, and homogenate is heated to 90 ℃ and keeps 2h, is cooled to room temperature, filters and removes precipitation; Filtrate adds 20% the macroporous resin account for cartilage weight, adopts sodium hydroxide to transfer pH 9.0, and stirring and adsorbing 4h. filters and collects resin, resin water flushing 3 times, with 3 mol/L sodium chloride solution eluting, eluent adds 3 times of volume ethanol, filter collecting precipitation, precipitation is collagen and mucopolysaccharide;
With shark cartilage organic calcium, collagen protein and mucopolysaccharide, with parts by weight shark cartilage organic calcium 20-80 part, collagen protein 1-20 part and mucopolysaccharide 1-20 part, be prepared into required dosage form at last.
2. shark cartilage according to claim 1 prepares the method for replenishing the calcium and promoting the product that cartilage metabolism is grown, it is characterized in that: when shark cartilage organic calcium, collagen protein and mucopolysaccharide are prepared into required dosage form, the material that also will add following parts by weight: calcareous short receipts agent phosphopeptide caseinate CPP 0.5-1.5 part, immune factor AKGs AKGS 0.0005-0.001 part, vitamin D 0.000002-0.000005 part and natural essence 0.1-0.3 part.
3. shark cartilage according to claim 1 prepares the method for replenishing the calcium and promoting the product that cartilage metabolism is grown, and it is characterized in that: dosage form is tablet or hard capsule or liquid capsule.
4. the replenishing the calcium and promote the product that cartilage metabolism is grown of shark cartilage preparation is characterized in that: made by claim 1,2 or 3 described methods.
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| CN2011100701690A CN102133231B (en) | 2011-03-23 | 2011-03-23 | Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof |
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| CN2011100701690A CN102133231B (en) | 2011-03-23 | 2011-03-23 | Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525876B (en) * | 2013-09-20 | 2015-10-28 | 中国海洋大学 | A kind of method of AKGs in enrichment shark liver oil |
| CN106072665A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof |
| CN108968075A (en) * | 2018-07-05 | 2018-12-11 | 天津市益倍建生物技术有限公司 | It is a kind of improve bone health composition and its application |
| CN111387397A (en) * | 2020-04-15 | 2020-07-10 | 湖北瑞邦生物科技有限公司 | Bovine bone peptide beverage for improving skeleton and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201666A (en) * | 1998-07-02 | 1998-12-16 | 深圳银健食品有限公司 | Capsule of cartilage powder of shart and preparation thereof |
| CN1415313A (en) * | 2002-10-31 | 2003-05-07 | 尹渭元 | Oral dosing calcium tablet produced from shark |
| CN101358220A (en) * | 2008-09-25 | 2009-02-04 | 王清荣 | Method for extracting calcium chondroitin sulfate in shark cartilage |
-
2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201666A (en) * | 1998-07-02 | 1998-12-16 | 深圳银健食品有限公司 | Capsule of cartilage powder of shart and preparation thereof |
| CN1415313A (en) * | 2002-10-31 | 2003-05-07 | 尹渭元 | Oral dosing calcium tablet produced from shark |
| CN101358220A (en) * | 2008-09-25 | 2009-02-04 | 王清荣 | Method for extracting calcium chondroitin sulfate in shark cartilage |
Non-Patent Citations (5)
| Title |
|---|
| 孟妍等.酪蛋白磷酸肽促进钙吸收效果的研究.《卫生研究》.2009,第38卷(第4期),489-491. * |
| 江龙法邓.鲨鱼软骨粘多糖的制备工艺研究.《淮海工学院学报》.2000,第9卷(第4期),49-52. * |
| 王林祥等.鲨鱼肝油中烷氧基甘油的成分分析.《无锡轻工大学学报》.2004,第23卷(第2期),90-93. * |
| 胡祖光等.鲨鱼软骨胶囊的药效学研究.《中药药理与临床》.1996,(第4期),38-40. * |
| 邓必阳邓.鲨鱼软骨营养成分分析及其评价.《营养学报》.1999,第21卷(第1期),104-108. * |
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