CN102049057A - Method for killing bacterial spores - Google Patents
Method for killing bacterial spores Download PDFInfo
- Publication number
- CN102049057A CN102049057A CN2010105937563A CN201010593756A CN102049057A CN 102049057 A CN102049057 A CN 102049057A CN 2010105937563 A CN2010105937563 A CN 2010105937563A CN 201010593756 A CN201010593756 A CN 201010593756A CN 102049057 A CN102049057 A CN 102049057A
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- China
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- concentration
- brood cell
- solution
- edta
- oxidant
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 210000004666 bacterial spore Anatomy 0.000 title abstract description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000007800 oxidant agent Substances 0.000 claims abstract description 11
- 230000001590 oxidative effect Effects 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 claims abstract description 5
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 5
- 229960003767 alanine Drugs 0.000 claims abstract description 5
- 244000144987 brood Species 0.000 claims description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 229960002089 ferrous chloride Drugs 0.000 claims description 2
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 2
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000000249 desinfective effect Effects 0.000 abstract 1
- 231100000053 low toxicity Toxicity 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 210000004215 spore Anatomy 0.000 abstract 1
- 230000003313 weakening effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 18
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 5
- 230000037396 body weight Effects 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000009849 deactivation Effects 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000005202 decontamination Methods 0.000 description 2
- 230000003588 decontaminative effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 231100000460 acute oral toxicity Toxicity 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- -1 hydroxyl radical free radical Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for killing bacterial spores. The method comprises the following steps: 1) dropping L-alanine serving as a spore germinator on a contaminated article; 2) then continuing dropping a strong alkaline solution serving as a resistance weakening agent; and 3) adjusting the pH value of the solution to 4.0-5.0 by taking H2O2, Fe2<+> and EDTA as a reaction system so as to prepare an oxidant; and dropping the prepared oxidant on the contaminated article. The method provided by the invention is used for disinfecting the contaminated environment and articles and killing the bacterial spores attached to the environment and articles, and has the advantages of good effect, low toxicity, little stimulus, low cost and the like.
Description
Technical field
The present invention relates to a kind of method of sterilization, particularly a kind of effectively method of deactivation bacterial spore.
Technical background
Bacterial spore has the resistant function of height to physical and chemical factor.Therefore, conventional means was to take to burn to add high concentration chemicals long period of soaking when decontamination was carried out in the plague area due to the bacterial spore, but these methods are very serious to the pollution of environment.Carried out on-the-spot anthrax test as near the Gruinard island nineteen forty-two Scotland seashore, island are seriously polluted, just are purified after adopting high-concentration formaldehyde to add the sea water mixing immersion treatment in 1986; 2003, what the anthrax grave of Three Gorge Reservoir Region in Chongqing was handled also employing was that high power amount bleaching powder and mixing with soil are soaked, then the method for gasoline burning.
The characteristics that attack according to bio-terrorism attack ground often in the place of crowd's high concentration in recent years, as build in interior, the means of transport and important traffic place, on-the-spot decontamination difficulty and require to increase; It is very necessary to set up situ purification treatment technology reliable, environment-friendly type.
Summary of the invention
The method that the purpose of this invention is to provide a kind of kill bacteria brood cell, described method is utilized inducing of germinant and drag attenuator, promote bacterial spore to start rudiment, reduce the drag of cell space structure and composition, make that its chitin under the further effect of oxidant breaks, nucleolysis, thalline be by effectively deactivation.The present invention is used for contaminated environment and sterilisation of objects, kills the bacterial spore that is attached on environment and the article, and has effectively, and toxicity is low, and zest is little, low cost and other advantages.
Technical scheme of the present invention is:
Kill bacteria brood cell's method has following steps:
1) on contaminated article, drip spill concentration be 0.10-0.40 mol L as the L-alanine of brood cell's germinant, effect 20-60min;
2) subsequently, on the contaminated article of handling through the described brood cell's germinant of step 1), continue to drip spill concentration be 0.02-0.50 mol L as the strong base solution of drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjusting solution is 4.0-5.0, promptly gets the preparation oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
Step 2) described strong base solution is strong base solutions such as sodium hydroxide or potassium hydroxide.
Step 3) is described; Fe
2+Be ferrous sulfate or solution of ferrous chloride.
Reagent described in brood cell's germinant of the present invention, drag attenuator (basifier) and the oxidant is the commercially available prod.
Germinant, drag attenuator mainly act on brood cell's chitin and brood cell's clothing of bacterial spore among the present invention, and it is damaged that it is produced, and eliminates barrier and protective effect, and brood cell's core exposes; Oxidant can produce the hydroxyl radical free radical with forceful electric power position, and oxidant has the forceful electric power position, therefore strong oxidation is arranged, and germination body nucleolysis, enzymatic activity are disappeared, and thalline thoroughly is inactivated.
The method that the present invention is used for the kill bacteria brood cell is hypotoxicity to animal, and corrosivity is little, cost is low, and is easy to use.Each constituent is preserved at room temperature and is got final product, good stability, during use with joining with usefulness.
The specific embodiment
The present invention is described further below in conjunction with example.
1. results of laboratory
With final concentration be 0.10 mol L L-alanine be the germinant main constituent, effect brood cell suspension 20-60min.The sodium hydroxide aqueous slkali that with concentration is 0.02-0.50 mol L is the drag attenuator, acts on above-mentioned brood cell's suspension 20-40min, with H
2O
2, Fe
2+, EDTA is reaction system, determines H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value 4.0-5.0, action time 30-60min.The result is as shown in table 1.
Brood cell's germinant of the present invention, drag attenuator (basifier) and oxidant, during use according to this method compound method, and according to sterilization steps of the present invention with joining with usefulness.
2. in-site modeling sterilization experiment
Get stone, tile, blade, cloth sheet, wooden unit surface (1x1cm area) drip brood cell's suspension and drip 10ul brood cell's suspension, get sandy soil 0.05g and drip 10ul brood cell's suspension, simulate contaminated external environment.Carry out brood cell's inactivation treatment with the inventive method:
With final concentration be 0.40 mol L L-alanine be germinant, drip 10ul in article surface, effect 20-60min;
The sodium hydroxide aqueous slkali that with concentration is 0.12-0.25 mol L is the drag attenuator, drips 90ul in above-mentioned article surface, effect 20-40min;
With H
2O
2, Fe
2+, EDTA is reaction system, determines H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value in reaction 4.0-5.0 drips 90ul in above-mentioned article surface, effect 30-60min.
The result is carried out count plate.Different analog sample brood cell deactivation the results are shown in Table 2.
3. act on the mixed liquor toxicity test
Choose 60 of the SPF level Wistar rats of body weight 180-200 g, be divided into experimental group and matched group, male and female half and half.Acute oral toxicity test is pressed the 1.00ml/100g body weight, with gastric infusion of used sterilization mixed liquor, observes 14d record body weight, diet, death condition continuously; Subacute per os toxicity test is pressed the 1.00ml/100g body weight, and every day, per os was irritated the stomach contamination, and negative control group gives sterile distilled water.Continuous contamination 28d, last contamination back 24h broken end is got blood, detects every routine blood test index, and major organs is carried out pathologic finding.
The result: all in this detection unit range of normal value, every index of each dosage group and matched group compare every index, the equal not statistically significant of difference.This concentration sterilization mixed liquor is nontoxic.
Claims (3)
1. a kill bacteria brood cell method is characterized in that, following steps are arranged:
1) on contaminated article, drip spill concentration be 0.10-0.40 mol L as the L-alanine of brood cell's germinant, effect 20-60min;
2) subsequently, on the contaminated article of handling through the described brood cell's germinant of step 1), continue to drip spill concentration be 0.02-0.50 mol L as the strong base solution of drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjusting solution is 4.0-5.0, promptly gets the preparation oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
2. kill bacteria brood cell's according to claim 1 method is characterized in that: step 2) described strong base solution is strong base solutions such as sodium hydroxide or potassium hydroxide.
3. kill bacteria brood cell's according to claim 1 method, it is characterized in that: step 3) is described; Fe
2+Be ferrous sulfate or solution of ferrous chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102049057A true CN102049057A (en) | 2011-05-11 |
| CN102049057B CN102049057B (en) | 2012-12-12 |
Family
ID=43954071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010593756 Expired - Fee Related CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102049057B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701881A (en) * | 2012-06-05 | 2012-10-03 | 凌天骏 | A kind of soil disinfectant and soil disinfection and sterilization method |
| CN105962002A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院农产品加工研究所 | Gentle way to kill bacterial spores |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
-
2010
- 2010-12-17 CN CN 201010593756 patent/CN102049057B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701881A (en) * | 2012-06-05 | 2012-10-03 | 凌天骏 | A kind of soil disinfectant and soil disinfection and sterilization method |
| CN102701881B (en) * | 2012-06-05 | 2014-05-14 | 凌天骏 | A kind of soil disinfectant and soil disinfection and sterilization method |
| CN105962002A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院农产品加工研究所 | Gentle way to kill bacterial spores |
| CN105962002B (en) * | 2016-05-05 | 2019-07-09 | 中国农业科学院农产品加工研究所 | The method that mild formula kills bacterial spore |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102049057B (en) | 2012-12-12 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121212 Termination date: 20141217 |
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