CN102004103B - Kit for the detection of tyrosine-like phenolic metabolites in human urine - Google Patents

Abstract

A kit for detecting tyrosine phenolic metabolites in human urine, comprising reagents, the reagents are 0.1-0.2g/ml mercury sulfate solution, 1-2g/ml mercury nitrate solution, 0.1-0.2g The nickel nitrate solution per ml is mixed by volume, i.e. mercuric nitrate solution: mercuric sulfate solution: nickel nitrate solution=1: 0.5～1.5: 0.5～2, and is a light green transparent liquid. The kit of the present invention is innovated on the basis of the principle of Millon reagent, which eliminates the interference of alkaline substances, metal ions and other related substances in urine on reagent detection, and improves the sensitivity and accuracy of detection.

Description

Kit for the detection of tyrosine-like phenolic metabolites in human urine

technical field

The invention relates to a kit for detecting tyrosine phenolic metabolites in human urine.

technical background

Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with advanced equipment. According to the research of the World Health Organization: 1/3 of cancers can be prevented at present; 1/3 of cancers can prolong the life of patients through active treatment; 1/3 of cancers can be cured through early detection, early diagnosis and early treatment of. The abnormal amino acid metabolism in the urine of cancer patients produces a large number of tyrosine-like phenolic metabolites. Whether the human body suffers from cancer can be deduced by detecting the content of tyrosine phenolic metabolites.

Contents of the invention

The object of the present invention is to provide a kit for detecting tyrosine-like phenolic metabolites in human urine with high detection sensitivity and good accuracy.

The kit for detecting tyrosine phenolic metabolites in human urine of the present invention includes reagents, the reagents are mercuric sulfate solution of 0.1-0.2 g/ml, mercuric nitrate solution of 1-2 g/ml, mercuric nitrate solution of 0.1-0.2 The nickel nitrate solution of g/ml is mixed according to the volume ratio, that is, mercury nitrate solution: mercury sulfate solution: nickel nitrate solution=1: 0.5～1.5: 0.5～2, and is a light green transparent liquid.

The preparation method of each component in the reagent: 0.1-0.2g/ml mercury sulfate solution is calculated according to the concentration, accurately weigh the amount of mercury sulfate (analytical pure) reagent, dissolve it in 3mol/L sulfuric acid solution, and prepare the concentration Mercury sulfate solution; 1-2g/ml mercury nitrate solution is reacted with 20g mercury and 30ml nitric acid whose specific gravity is 1.4. After the mercury is completely dissolved, add distilled water 1-2 times the volume of the reaction product to prepare The mercury nitrate solution of this concentration; the nickel nitrate solution of 0.1-0.2g/ml is calculated according to the concentration, accurately takes by weighing the amount of nickel nitrate (analytical pure) reagent, is dissolved in distilled water, is mixed with the nickel nitrate solution of this concentration.

The test kit for detecting tyrosine-like phenolic metabolites in human urine of the present invention, the detection method: take 3ml of the subject's urine, add it dropwise to a test tube containing 0.5ml of this reagent, and let it stand for 2-5 Minutes, precipitation and color reaction occur, judge according to the presence or absence of precipitation and the color of precipitation.

Judgment criteria: 1. If there is no precipitation in the reaction, or if there is precipitation, the color is white or yellow, it is judged as negative, indicating that there is no cancer in the body; 2. If the reaction has precipitation, the color is rose red or brick red, it is judged as positive, indicating that there is no cancer in the body. have cancer.

Medical and clinical studies have shown that the high reproduction rate and high metabolic rate of cancer cells cause a large number of amino acid (tyrosine) metabolites containing phenolic nucleus to appear in the urine of cancer patients, while normal people's urine does not appear or only contains very low Concentration of such amino acid metabolites, therefore, tyrosine phenolic metabolites become one of the tumor markers. The mercury salt in this reagent reacts specifically with this tumor marker, and the reaction product generates a red precipitate through molecular isomerization and oxidation, but it reacts with normal human urine without precipitation or the precipitate is yellow, which can be identified by the precipitate and its color Determine the presence or absence of such tumor markers, and then infer whether they carry cancer cells, that is, whether they are cancer patients.

The kit for detecting tyrosine-like phenolic metabolites in human urine of the present invention is an innovation based on the principle of Millon reagent, which eliminates the detection of alkaline substances, metal ions and other related substances in urine. According to the clinical verification of the Cancer Hospital, 278 of the 300 cases of cancer patients diagnosed by histology were positive, and the accuracy rate (coincidence rate) reached 92.6%.

Detailed ways

A kit for detecting tyrosine-like phenolic metabolites in human urine, the reagent method for preparing 1000ml of the kit:

The reagent is composed of three kinds of solutions, according to the volume ratio, that is, mercury nitrate solution: mercury sulfate solution: nickel nitrate solution = 1:1:2, respectively prepared as 250ml, 250ml and 500ml, and the three solutions are mixed evenly to prepare 1000ml. reagent.

The preparation method of each component is as follows:

1. The concentration of mercury nitrate solution is 1g/ml. Weigh 64g of mercury and dissolve it in 96ml of nitric acid with a specific gravity of 1.4. After the mercury is completely dissolved, add 166.7ml of distilled water to prepare 250ml of mercury nitrate solution with a concentration of 1g/ml;

2. The concentration of mercuric sulfate solution is 0.1g/ml. Weigh 25g of mercuric sulfate reagent, dissolve it in a small amount of 3mol/L sulfuric acid solution, and then add 3mol/L sulfuric acid solution to 250ml to prepare a concentration of 0.1g/ml. ml of mercury sulfate solution;

3. The concentration of nickel nitrate solution is 0.1g/ml. Weigh 80g of nickel nitrate hexahydrate, dissolve it in a small amount of distilled water, and then add distilled water to 500ml to prepare a nickel nitrate solution with a concentration of 0.1g/ml.

Claims (1)

1. A test kit for detecting tyrosine phenolic metabolites in human urine, comprising reagents, characterized in that: the reagents are composed of mercuric sulfate solution of 0.1-0.2g/ml, mercuric nitrate of 1-2g/ml Solution, the nickel nitrate solution of 0.1-0.2g/ml is by volume ratio, namely mercuric nitrate solution: mercuric sulfate solution: nickel nitrate solution=1: 0.5～1.5: 0.5～2, mixes, and is light green transparent liquid; Wherein The preparation method of 1-2g/ml mercury nitrate solution is: react with 20g of mercury and 30ml of nitric acid whose specific gravity is 1.4, after the mercury is completely dissolved, add distilled water 1-2 times the volume of the reaction product to prepare Mercury nitrate solution of this concentration.