CN101975862A - Test strip for measuring protein content of food and testing method thereof - Google Patents
Test strip for measuring protein content of food and testing method thereof Download PDFInfo
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- CN101975862A CN101975862A CN 201010522029 CN201010522029A CN101975862A CN 101975862 A CN101975862 A CN 101975862A CN 201010522029 CN201010522029 CN 201010522029 CN 201010522029 A CN201010522029 A CN 201010522029A CN 101975862 A CN101975862 A CN 101975862A
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Abstract
本发明提供一种用于食品中蛋白质含量测定的检测试条及其检测方法，本发明检测试条包括过滤带、反应带、吸水带和基片。反应带中吸附有指示剂，可与样品中蛋白质进行特异性反应，通过颜色变化指示蛋白质的含量。用此试条通过便携式测色仪检测蛋白质，检测时间短，一般低于30秒，结果准确，能够实现食品中蛋白质的现场快速检测。
The invention provides a detection test strip for measuring protein content in food and a detection method thereof. The detection test strip comprises a filter belt, a reaction belt, a water absorption belt and a substrate. The indicator is adsorbed in the reaction zone, which can specifically react with the protein in the sample, and the protein content is indicated by the color change. Using this test strip to detect protein with a portable colorimeter, the detection time is short, generally less than 30 seconds, the result is accurate, and the on-site rapid detection of protein in food can be realized.
Description
Technical field
The present invention relates to the detection technique of protein content in a kind of fast measuring food, belong to the food safety detection field.
Background technology
Food is the elementary necessaries that people live, and the protein in the food is unique source of nitrogen in the human body.Therefore, what of protein content in the food are not only represented the quality of food, and are concerning the health of human body.National food hygienic standard has also been made respective specified to Protein content in the part food, wherein, in animal breast and the animal dairy products protein measuring be analyzing food nutrition components of mirowave must survey project.Current, the milk powder quality determination methods of current consumption person and quality testing department field by using mainly relies on observation of use senses's (feel, color, smell, taste etc.), the quality testing department random sampling is delivered to the technique center and is detected then, and protein measuring adopts national standard method more---Kjeldahl.Though yet observation of use senses's easy operating, subjectivity is strong, accuracy is low, is a kind of preliminary detection means; And its ultimate principle of traditional Kjeldahl is with food and sulfuric acid and catalyzer hot digestion together, and protein is decomposed, and the ammonia of decomposition combines generation ammonium sulfate with sulfuric acid.Alkalization distillation makes ammonia free then, again with sulfuric acid or hydrochloric acid standard solution titration, multiply by reduction coefficient according to the consumption of acid after absorbing with boric acid, is protein content.Clearly, the result who utilizes Kjeldahl to record not exclusively is a protein, the nitrogen substance that also comprises some nonprotein classes, as urea nitrogen, free ammonia nitrogen, inorganic ammonia salt etc., causing the lawless person to have an opportunity to take advantage of, in raw material, mix a large amount of extract of protein---melamine is to improve powdered milk protein content, to illegitimately make exorbitant profits, thereby cause shocking the outburst of national " Sanlu milk powder " incident, caused irremediable loss to the country and people.As seen, what classic method was difficult to solve protein in the milk mixes a dummy check difficult problem, also has shortcomings such as operating process is loaded down with trivial details, time and effort consuming simultaneously, and can produce a large amount of harmful gases and contaminated environment in operating process, influence operator ' s health, can not satisfy the needs of fast measuring.
Summary of the invention
Purpose of the present invention, be exactly deficiency, provide a kind of test-strips and strip detection method that is used for food protein fast detecting, make it improve detection efficiency to food safety detection department at above-mentioned method of testing, reduce cost, effectively shorten sample preparation time and detection time.Detect protein with this strip, sample preparation is simple, and detection time is short, generally is lower than 30 seconds, can realize the fast detecting of protein content in the food.
In order to achieve the above object, the present invention realizes like this, described detection strip comprises filter bag, reaction zone, water sucting belt and substrate, the reaction zone that at first will be adsorbed with indicator is bonded on the substrate, filter bag and water sucting belt place the both sides of reaction zone respectively, and be connected mutually, thereby obtain detection strip of the present invention with the reaction zone two ends.Accurately pipetting 20 μ L sample solutions with pipettor drips in strip filter bag central authorities, arrest reaction is 30 seconds under the room temperature, sharp portable colour photometer MICG1A (Japanese SATAKE company product) measures the reflectivity of strip reaction zone after 30 seconds, according to the proportional relationship between reflectivity and protein concentration, judge Protein content in the powdered milk sample, thereby obtain strip detection method of the present invention.
Described filter bag, thickness are 0.1～0.5mm, by a kind of composition the in poly tetrafluoroethylene, glass fibre membrane, polypropylene screen, louver film, the nylon membrane, preferred glass fibers film and louver film.
Described reaction zone, thickness are 100～200nm, by a kind of composition the in nitrocellulose filter, cellulose acetate membrane, mixed cellulose ester membrane, poly (ether sulfone) film, the polyvinylidene fluoride film, and preferred nitrocellulose filter, cellulose acetate membrane and mixed cellulose ester membrane.
The indicator that adsorbs in the described reaction zone, form preferred tetrabromophenol sulfonphthalein, acid blue 90 and dibromophenolphthalein by at least a in tetrabromophenol sulfonphthalein, acid blue 90, phenol red, cresol red, chlorophenesic acid sulphonyl phthalein, dibromophenolphthalein and the cresol-purple or more than one.
Described water sucting belt, thickness are 1～10mm, a kind of composition in quantitative filter paper, qualitative filter paper, suction cotton paper, active carbon filter paper, the synthon filter paper, preferred quantitative filter paper, qualitative filter paper and suction cotton paper.
Described strip detection method is, the reaction zone that is adsorbed with indicator is bonded on the substrate, and filter bag and water sucting belt place the both sides of reaction zone respectively, and are connected mutually with the reaction zone two ends, thereby make the protein detection strip.Accurately pipetting 20 μ L sample solutions with pipettor drips in strip filter bag central authorities, arrest reaction is 30 seconds under the room temperature, sharp portable colour photometer MICG1A measures the reflectivity of strip reaction zone after 30 seconds, according to the proportional relationship between reflectivity and protein concentration, judge Protein content in the powdered milk sample.
Compare with protein content detection technique in the existing food, the present invention detects strip and has advantages such as sample preparation is simple, detection speed is fast, easy to use, be well suited for the field quick detection of protein content in the food, can improve the testing efficient of Food Inspection quarantine departments effectively, shorten sense cycle greatly.
Description of drawings
Fig. 1 is the detection strip structural representation that the present invention is used for the food protein determination.
Embodiment
The invention provides a kind of detection strip and detection method thereof that is used for the food protein determination, specific implementation process is: detect strip according to the present invention shown in Figure 1 and be made up of filter bag 1, reaction zone 3, water sucting belt 4 and substrate 2 four parts, the reaction zone 3 that at first will be adsorbed with indicator is bonded on the substrate 2, filter bag 1 and water sucting belt 4 place the both sides of reaction zone 3 respectively, and be connected mutually, thereby make the protein detection strip with reaction zone 3 two ends.
Described filter bag 1 is by a kind of composition the in poly tetrafluoroethylene, glass fibre membrane, polypropylene screen, louver film, the nylon membrane, preferred glass fibers film and louver film, and thickness is 0.1～0.5mm.
Described reaction zone 3 is by a kind of composition the in nitrocellulose filter, cellulose acetate membrane, mixed cellulose ester membrane, poly (ether sulfone) film, the polyvinylidene fluoride film, preferred nitrocellulose filter, cellulose acetate membrane and mixed cellulose ester membrane, and thickness is 100～200nm.
The indicator of absorption is formed preferred tetrabromophenol sulfonphthalein, acid blue 90 and dibromophenolphthalein by at least a in tetrabromophenol sulfonphthalein, acid blue 90, phenol red, cresol red, chlorophenesic acid sulphonyl phthalein, dibromophenolphthalein and the cresol-purple or more than one in the described reaction zone 3.
Described water sucting belt 4, a kind of composition in quantitative filter paper, qualitative filter paper, suction cotton paper, active carbon filter paper, the synthon filter paper, preferred quantitative filter paper, qualitative filter paper and suction cotton paper, thickness is 1～10mm.Present invention is described below in conjunction with specific embodiment:
Embodiment 1: the fast detecting of protein in the milk powder
1, accurately takes by weighing the 100mg powdered milk sample, be dissolved in the phosphate buffer of 1000 μ L pH7.8, mix;
2, get a homemade protein detection strip, keep flat and place on the monitor station;
3, accurately pipette 20 μ L sample solutions with pipettor and drip in strip filter bag central authorities, arrest reaction is 30 seconds under the room temperature;
4, utilize portable colour photometer MICG1A to measure the reflectivity of strip reaction zone after 30 seconds;
5,, judge Protein content in the powdered milk sample according to the proportional relationship between reflectivity and protein concentration.
Above embodiment is just in order to illustrate the present invention, rather than limitation of the present invention; On the basis of the above description, the present invention can be many modifications and changes.Within the scope of the appended claims, the present invention can have and is different from other above-mentioned implementation, selects other material for use, adjusts methods such as reaction time all within patent claimed range of the present invention.
Claims (5)
1. be used for the detection strip of food protein content determination, it is characterized in that: detect strip and form, wherein be adsorbed with indicator in the reaction zone by filter bag, reaction zone, water sucting belt and substrate four parts.
2. the detection strip that is used for the food protein content determination according to claim 1, it is characterized in that: described filter bag can be a kind of in poly tetrafluoroethylene, glass fibre membrane, polypropylene screen, louver film, the nylon membrane, and thickness is 0.1～0.5mm.
3. the detection strip that is used for the food protein content determination according to claim 1, it is characterized in that: described reaction zone can be a kind of in nitrocellulose filter, cellulose acetate membrane, mixed cellulose ester membrane, poly (ether sulfone) film, the polyvinylidene fluoride film, preferred nitrocellulose filter, cellulose acetate membrane and mixed cellulose ester membrane, thickness is 100～200nm; The indicator that adsorbs in the reaction zone, form preferred tetrabromophenol sulfonphthalein, acid blue 90 and dibromophenolphthalein by at least a in tetrabromophenol sulfonphthalein, acid blue 90, phenol red, cresol red, chlorophenesic acid sulphonyl phthalein, dibromophenolphthalein and the cresol-purple or more than one.
4. the detection strip that is used for the food protein content determination according to claim 1, it is characterized in that: described water sucting belt can be a kind of in quantitative filter paper, qualitative filter paper, suction cotton paper, active carbon filter paper, the synthon filter paper, preferred quantitative filter paper, qualitative filter paper and suction cotton paper, thickness is 1～10mm.
5. the strip detection method that is used for the food protein determination, it is characterized in that: the reaction zone that will be adsorbed with indicator is bonded on the substrate, filter bag and water sucting belt place the both sides of reaction zone respectively, and be connected mutually with the reaction zone two ends, thereby make the protein detection strip, accurately pipetting 20 μ L sample solutions with pipettor drips in strip filter bag central authorities, arrest reaction is 30 seconds under the room temperature, utilize portable colour photometer to measure the reflectivity of strip reaction zone after 30 seconds, according to the proportional relationship between reflectivity and protein concentration, judge Protein content in the powdered milk sample.
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| CN 201010522029 CN101975862B (en) | 2010-10-28 | 2010-10-28 | Detection test strip and detection method for protein content determination in food |
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| CN 201010522029 CN101975862B (en) | 2010-10-28 | 2010-10-28 | Detection test strip and detection method for protein content determination in food |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221547A (en) * | 2011-06-16 | 2011-10-19 | 河南工业大学 | Device and method for detecting Beta-lactamase in raw milk and dairy product |
| CN105651765A (en) * | 2015-12-28 | 2016-06-08 | 东北农业大学 | Rapid protein test paper, and production method and detection method thereof |
| CN106959235A (en) * | 2017-05-26 | 2017-07-18 | 同昕生物技术（北京）有限公司 | A kind of blood preseration device |
| CN108645846A (en) * | 2018-05-15 | 2018-10-12 | 长春比特医疗仪器有限公司 | A method of serum alpha-amylase activity is measured based on paper substrate |
| CN109339023A (en) * | 2018-09-29 | 2019-02-15 | 湖南大学 | A geogrid with drainage function |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101526467A (en) * | 2009-04-17 | 2009-09-09 | 湖南大学 | Optical biological sensing technology based on interaction between terminal-protected analyzed detected small molecules of single-chain exonuclease I and combined protein |
| CN101865853A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof |
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2010
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101526467A (en) * | 2009-04-17 | 2009-09-09 | 湖南大学 | Optical biological sensing technology based on interaction between terminal-protected analyzed detected small molecules of single-chain exonuclease I and combined protein |
| CN101865853A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《中国优秀博硕士学位论文全文数据库（硕士）工程科技I辑》 20030615 杨平华 蛋白质的光分析化学特性研究 第5-6页 3,5 , 第2期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221547A (en) * | 2011-06-16 | 2011-10-19 | 河南工业大学 | Device and method for detecting Beta-lactamase in raw milk and dairy product |
| CN105651765A (en) * | 2015-12-28 | 2016-06-08 | 东北农业大学 | Rapid protein test paper, and production method and detection method thereof |
| CN106959235A (en) * | 2017-05-26 | 2017-07-18 | 同昕生物技术（北京）有限公司 | A kind of blood preseration device |
| CN106959235B (en) * | 2017-05-26 | 2019-08-09 | 同昕生物技术（北京）有限公司 | A kind of blood preseration device |
| CN108645846A (en) * | 2018-05-15 | 2018-10-12 | 长春比特医疗仪器有限公司 | A method of serum alpha-amylase activity is measured based on paper substrate |
| CN109339023A (en) * | 2018-09-29 | 2019-02-15 | 湖南大学 | A geogrid with drainage function |
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Inventor after: He Baoshan Inventor after: Xie Lingling Inventor after: Li Xiaoyan Inventor after: Xie Yanli Inventor after: Li Xueqin Inventor after: Han Xiaoxian Inventor after: Li Mannan Inventor before: He Baoshan Inventor before: Xie Yanli Inventor before: Li Xueqin Inventor before: Han Xiaoxian Inventor before: Li Mannan |
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