CN101974404A - Experimental device and process for two-way perfusion mechanics - Google Patents
Experimental device and process for two-way perfusion mechanics Download PDFInfo
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- CN101974404A CN101974404A CN2010102882588A CN201010288258A CN101974404A CN 101974404 A CN101974404 A CN 101974404A CN 2010102882588 A CN2010102882588 A CN 2010102882588A CN 201010288258 A CN201010288258 A CN 201010288258A CN 101974404 A CN101974404 A CN 101974404A
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- 230000010412 perfusion Effects 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title abstract description 4
- 239000000463 material Substances 0.000 claims abstract description 21
- 238000002474 experimental method Methods 0.000 claims abstract description 20
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims description 16
- 238000005516 engineering process Methods 0.000 claims description 12
- 210000004409 osteocyte Anatomy 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 210000002449 bone cell Anatomy 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000000741 silica gel Substances 0.000 abstract 1
- 229910002027 silica gel Inorganic materials 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 14
- 239000012620 biological material Substances 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002188 osteogenic effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000005222 synovial tissue Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/12—Pulsatile flow
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Abstract
本发明公开了一种双向灌流力学实验装置及工艺，包括蠕动泵、流体压力传导室，其特征在于：蠕动泵与流体压力传导室通过管道相连；流体压力传导室有3～6个充满骨细胞/培养液的培养室，培养室被硅胶膜包裹，里面可以放入需要实验的支架材料，在培养室上部有透气盖。本发明可以满足三维动态流体微环境对三维生物支架材料的疲劳力学研究实验，可获得生物支架材料的设计与制备所需要的实验数据。
The invention discloses a two-way perfusion mechanics experiment device and process, which includes a peristaltic pump and a fluid pressure conduction chamber, and is characterized in that: the peristaltic pump and the fluid pressure conduction chamber are connected through pipelines; the fluid pressure conduction chamber has 3 to 6 bone cells filled /Cultivation chamber of culture medium, the cultivation chamber is wrapped by a silica gel membrane, and the support material required for experiment can be placed in it, and there is a ventilated cover on the upper part of the cultivation chamber. The invention can meet the fatigue mechanics research experiment of the three-dimensional dynamic fluid microenvironment on the three-dimensional biological support material, and can obtain the experimental data required for the design and preparation of the biological support material.
Description
Technical field
The present invention relates to a kind of experimental installation and technology of the mechanics of materials, specifically, is a kind of two-way perfusion Experiments of Machanics device and the technology that is used for the biomaterial experiment.
Background technology
Along with biological development, cell micro-environment more and more comes into one's own to the influence of the growth of biomaterial biocompatibility and cell, and particularly mechanical stimulation is to the influence research of biomaterial and cell.For this reason, many Experiments of Machanics devices have been invented.
Chinese patent CN1846606A (2006) discloses a kind of compound mechanical stress cell loading device for simulated joint cavity, the mechanical stress that simulated joint cavity synovial tissue is born under physiological situation, both can apply pulsating fluid shearing force, tensile stress or stress, surging force separately, can apply two again, three kind of mechanical stress bonded compound stress.Chinese patent CN1847847A (2006) discloses a kind of cyto-mechanics loading system, can produce planar equal-biaxial periodical pull stress or stress.Chinese patent CN101298592A (2008) discloses a kind of cell tensile loader, have control section, mechanical part and electromagnet portion, it be with the cell kind on the flexible silicon glued membrane, make cell carry out circumferential stretching/compression-loaded by power system then, simultaneously, use bag to be adhered to cell surface by integrin family by the magnetic bead collagen of Z 250, again in alternating magnetic fields of bottom-loaded up and down of cell cultures box, make cell in the substrate strain field except be subjected to can also be at tensile cell under the magneticaction the hoop stress.A common shortcoming of these mechanical loading units is exactly: can not carry out the experiment at the perfusion mechanics of the cell of biologic bracket material and growth, and the apparatus structure relative complex is made relatively difficulty.
Summary of the invention
The objective of the invention is above weak point, propose a kind of perfusion mechanical stimulation is tired to biomaterial and the cell growth effect is studied experimental installation and technology at existing mechanical loading unit.
In order to address the above problem, technical scheme of the present invention is: a kind of two-way perfusion Experiments of Machanics device and technology, comprise peristaltic pump, hydrodynamicpressure conduction chamber, and it is characterized in that: peristaltic pump links to each other by pipeline with hydrodynamicpressure conduction chamber; There are 3～6 culturing room that are full of osteocyte/nutrient solution hydrodynamicpressure conduction chamber, and culturing room is wrapped up by pellosil, and the timbering material that needs experiment can be put in the inside, on culturing room top ventilating cover is arranged.
In the technique scheme, air is arranged at described hydrodynamicpressure conduction top, chamber.
As the preferred embodiment of technique scheme, described peristaltic pump can conduct the chamber to hydrodynamicpressure and move in circles and be pressed into and emit sterile purified water.
Compared with prior art, the advantage of tool of the present invention is: the present invention has imitated real complicated and diversified mechanical environment in the body, when can realize culturing cell, realizes the influence research that controlled perfusion mechanical stimulation is tired to biomaterial and cell is grown.
Description of drawings
Fig. 1 is the two-way perfusion Experiments of Machanics of the present invention schematic representation of apparatus.
1. peristaltic pumps among the figure; 2. sterile purified water; 3. pellosil; 4. osteocyte/nutrient solution; 5. timbering material; 6. culturing room; 7. ventilating cover; 8. air; 9. hydrodynamicpressure conducts the chamber.
Embodiment
Further the present invention is illustrated below in conjunction with drawings and Examples.
Referring to Fig. 1, a kind of two-way perfusion Experiments of Machanics device and technology comprise peristaltic pump 1, hydrodynamicpressure conduction chamber 9, it is characterized in that: peristaltic pump 1 and hydrodynamicpressure conduction chamber 9 link to each other by pipeline; There are 3～6 culturing room 6 that are full of osteocyte/nutrient solution 4 hydrodynamicpressure conduction chamber 9, and culturing room 6 is wrapped up by pellosil 3, and the timbering material 5 that needs experiment can be put in the inside, on culturing room 6 tops ventilating cover 7 is arranged.Air 8 is arranged at hydrodynamicpressure conduction 9 tops, chamber.Peristaltic pump 1 can conduct chamber 9 to hydrodynamicpressure and move in circles and be pressed into and emit sterile purified water 2.
Concrete enforcement: culturing room 6 and hydrodynamicpressure conduction chamber 9 usefulness transparent plasticss are made, and by assembling of stainless steel screw and reinforcement material, culturing room 6 sides are by sealing by pellosil 3, pellosil 3 outsides are hydrodynamicpressure conduction chamber 9, and conduction chamber 9 is connected with peristaltic pump 1 by silicone tube, the inside is with being full of sterile purified water 2, and from air 8 Ex-all all gas.When carrying out the perfusion cultivation, start peristaltic pump 1, therefore liquid with pattern that can accurate control, come and go perfusion and produce alternating pressures in hydrodynamicpressure conduction chamber 9, drive pellosil 3 movement therewith, thus the osteocyte/nutrient solution 4 in the culturing room 6 will be in porous support materials 5 perfusion up and down reciprocatingly.
Open the ventilating cover 7 of culturing room 6 and put into timbering material 5, to contain rat scleroblast suspension of former generation, slowly splash into after 5 kinds of timbering materials plant from timbering material 5 end faces, open peristaltic pump 1 by above-mentioned steps, make cell suspension slowly reciprocal perfusion in timbering material 5, seed cell can be uniformly distributed in the timbering material 5 cultivate, simultaneously, carry out the fatigue mechanics research experiment of Three-Dimensional Dynamic fluid microenvironment three-dimensional biologic bracket material 5.
In conjunction with the data in the experiment, and with the static cultivation control group relatively, analyzing three-dimensional dynamic fluid microenvironment is to the influence of scleroblast vital movement and osteogenic ability, disclose three-dimensional biologic bracket material 5 fatigue mechanism, disclose the mechanics biology interaction mechanism between osteocyte/three-dimensional stent material 5, instruct the design and the preparation of biologic bracket material 5 theoretically.
Claims (4)
1. two-way perfusion Experiments of Machanics device and technology comprise peristaltic pump (1), hydrodynamicpressure conduction chamber (9), and it is characterized in that: peristaltic pump (1) conducts chamber (9) with hydrodynamicpressure and links to each other by pipeline; There are 3～6 culturing room (6) that are full of osteocyte/nutrient solution (4) hydrodynamicpressure conduction chamber (9), and culturing room (6) is by pellosil (3) parcel, and the timbering material (5) that needs experiment can be put in the inside, on culturing room (6) top ventilating cover (7) is arranged.
2. a kind of two-way perfusion Experiments of Machanics device according to claim 1 and technology is characterized in that: air (8) is arranged at hydrodynamicpressure conduction top, chamber (9).
3. a kind of two-way perfusion Experiments of Machanics device according to claim 1 and technology is characterized in that: peristaltic pump (1) can conduct chamber (9) to hydrodynamicpressure and move in circles and be pressed into and emit sterile purified water (2).
4. two-way perfusion Experiments of Machanics device and technology, its technology characteristics is: when carrying out the perfusion cultivation, start peristaltic pump (1), therefore liquid is with pattern that can accurate control, come and go perfusion and conduct chamber (9) generation alternating pressure in hydrodynamicpressure, drive pellosil (3) movement therewith, thus the osteocyte/nutrient solution (4) in the culturing room (6) will be porous support materials (5) in about, about reciprocal perfusion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102882588A CN101974404A (en) | 2010-09-21 | 2010-09-21 | Experimental device and process for two-way perfusion mechanics |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102882588A CN101974404A (en) | 2010-09-21 | 2010-09-21 | Experimental device and process for two-way perfusion mechanics |
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| CN101974404A true CN101974404A (en) | 2011-02-16 |
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| CN2010102882588A Pending CN101974404A (en) | 2010-09-21 | 2010-09-21 | Experimental device and process for two-way perfusion mechanics |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1635108A (en) * | 2004-11-25 | 2005-07-06 | 西安交通大学 | A rotary perfusion bioreactor system |
| CN1706936A (en) * | 2004-06-08 | 2005-12-14 | 中国人民解放军军事医学科学院基础医学研究所 | A perfusion bioreactor |
| CN101402952A (en) * | 2007-12-26 | 2009-04-08 | 北京航空航天大学 | Low-frequency high-breadth overlapped high-frequency low-breadth loading method and bioreactor thereof |
| CN101423820A (en) * | 2008-11-28 | 2009-05-06 | 浙江大学 | Osseous tissue sublevel perfusion culture method based on medulla ossium mesenchymal stem cell |
| CN201834910U (en) * | 2010-09-21 | 2011-05-18 | 重庆文理学院 | Two-way perfusion mechanical experiment device |
-
2010
- 2010-09-21 CN CN2010102882588A patent/CN101974404A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706936A (en) * | 2004-06-08 | 2005-12-14 | 中国人民解放军军事医学科学院基础医学研究所 | A perfusion bioreactor |
| CN1635108A (en) * | 2004-11-25 | 2005-07-06 | 西安交通大学 | A rotary perfusion bioreactor system |
| CN101402952A (en) * | 2007-12-26 | 2009-04-08 | 北京航空航天大学 | Low-frequency high-breadth overlapped high-frequency low-breadth loading method and bioreactor thereof |
| CN101423820A (en) * | 2008-11-28 | 2009-05-06 | 浙江大学 | Osseous tissue sublevel perfusion culture method based on medulla ossium mesenchymal stem cell |
| CN201834910U (en) * | 2010-09-21 | 2011-05-18 | 重庆文理学院 | Two-way perfusion mechanical experiment device |
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Application publication date: 20110216 |