CN101955320A - Method for modifying slide for liquid-based cytodiagnosis - Google Patents
Method for modifying slide for liquid-based cytodiagnosis Download PDFInfo
- Publication number
- CN101955320A CN101955320A CN 201010265545 CN201010265545A CN101955320A CN 101955320 A CN101955320 A CN 101955320A CN 201010265545 CN201010265545 CN 201010265545 CN 201010265545 A CN201010265545 A CN 201010265545A CN 101955320 A CN101955320 A CN 101955320A
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- China
- Prior art keywords
- slide
- liquid based
- modifying method
- based cytology
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 title claims abstract description 19
- 238000002444 silanisation Methods 0.000 claims abstract description 14
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 20
- 238000003745 diagnosis Methods 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 3
- 150000001299 aldehydes Chemical class 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 3
- 229910000077 silane Inorganic materials 0.000 claims description 3
- 238000005406 washing Methods 0.000 abstract description 2
- 238000007037 hydroformylation reaction Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 9
- 239000011521 glass Substances 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a method for modifying a slide for liquid-based cytodiagnosis, which comprises the following steps of: slide washing, slide silanization and slide hydroformylation. The slide surface modified by the method is relatively rough, so that the problem that the surface is smooth and low in adhesion to cells in the prior art is solved.
Description
Technical field
The invention belongs to the liquid based cytology diagnostic field, particularly relate to the modifying method of a kind of liquid based cytology diagnosis with slide.
Background technology
When adopting liquid-based cytology detection method, sample to be checked need be formed cell thin on slide, dye the sheet operation again.This needs surface special-purpose anticreep slide through modifying, and surface of glass slide can be combined closely with the cell surface material after chemically modified, with prevent cell in film-making, dye in the process such as sheet and come off, cause fail to pinpoint a disease in diagnosis, mistaken diagnosis.Traditional anticreep slide adopts 3-aminopropyl-3-(ethoxymethyl) silane (APES) or poly-lysine to modify, and the surface of glass slide after it is modified is level and smooth, causes the adhesive power of its pair cell not strong.
Summary of the invention
The purpose of this invention is to provide the modifying method of a kind of liquid based cytology diagnosis with slide, it is level and smooth to have solved the surface of glass slide that original technology modifies out, the problem that the adhesive power of pair cell is not strong.
To achieve these goals, the technical scheme that the present invention proposes is: a kind of liquid based cytology diagnosis modifying method of slide may further comprise the steps:
(1) cleaning of slide: slide is dipped in the NaOH solution, soaks with putting into HCl again after the distilled water cleaning behind the taking-up slide, clean with distilled water again behind the taking-up slide;
(2) silanization of slide: slide is put into silanization solution, puts into acetone soln again behind the taking-up slide;
(3) aldehyde radicalization of slide: slide is put into glutaraldehyde solution, wash with distillation behind the taking-up slide.
The concentration of NaOH and HCl is more than or equal to 1mol/L in the described step (1).Silanization solution in the described step (2) is made up of APES and acetone.The volume ratio of APES and acetone is 1: 55 in the silanization solution in the described step (2).The time that slide reacts in silanization solution in the described step (2) is 10～15 minutes.The time that slide stops in acetone soln in the described step (2) is 3～10 seconds.The concentration of glutaraldehyde solution is 4～8% in the described step (3).The time that slide reacts in glutaraldehyde solution in the described step (3) is 20～40 minutes, and temperature of reaction is 18～30 ℃.
Advantage of the present invention is: earlier slide is carried out the APES silanization and handle, make its surface with going up a large amount of positively charged ions, these cation easilies are convenient to the adhesion of cell in combining with the negatively charged ion of cell surface.After APES handles, again slide is carried out the aldehyde radical modification, make surface of glass slide coarse relatively thus, strengthened the adhesive capacity of slide pair cell.
Embodiment
A kind of liquid based cytology diagnosis of embodiment modifying method of slide may further comprise the steps:
(1) cleaning of slide: slide is dipped in the NaOH solution of 2mol/L, soaks with the HCl that puts into 2mol/L after the distilled water cleaning again behind the taking-up slide, clean with distilled water again behind the taking-up slide;
(2) silanization of slide: the volume ratio that slide is put into APES and acetone is 1: 55 a silanization solution 13 minutes, takes out to put into acetone soln again behind the slide and stopped 5 seconds;
(3) aldehyde radicalization of slide: it is that 5% glutaraldehyde solution reacted 35 minutes under 25 ℃ temperature that slide is put into concentration, takes out behind the slide with distillation washing 2 times.
The surface of glass slide of handling with this method is coarse relatively, has strengthened the adhesive capacity of slide pair cell.
Claims (10)
1. a liquid based cytology is diagnosed the modifying method of using slide, it is characterized in that may further comprise the steps:
(1) cleaning of slide: slide is dipped in the NaOH solution, soaks with putting into HCl again after the distilled water cleaning behind the taking-up slide, clean with distilled water again behind the taking-up slide;
(2) silanization of slide: slide is put into silanization solution, puts into acetone soln again behind the taking-up slide;
(3) aldehyde radicalization of slide: slide is put into glutaraldehyde solution, wash with distillation behind the taking-up slide.
2. according to the modifying method of the described a kind of liquid based cytology diagnosis of claim 1 with slide, the concentration that it is characterized in that described NaOH of step (1) and HCl is more than or equal to 1mol/L.
3. according to the modifying method of the described a kind of liquid based cytology diagnosis of claim 1, it is characterized in that the described silanization solution of step (2) is made up of 3-aminopropyl-3-(ethoxymethyl) silane and acetone with slide.
4. according to the modifying method of the described a kind of liquid based cytology diagnosis of claim 3, it is characterized in that the volume ratio 1: 55 of 3-aminopropyl-3-(ethoxymethyl) silane and acetone in the described silanization solution of step (2) with slide.
5. use the modifying method of slide according to claim 1,2,3 or 4 described a kind of liquid based cytology diagnosis, it is characterized in that the time that slide reacts in the described step (2) is 10～15 minutes in silanization solution.
6. use the modifying method of slide according to claim 1,2,3 or 4 described a kind of liquid based cytology diagnosis, it is characterized in that the time that described step (2) slide stops is 3～10 seconds in acetone soln.
7. use the modifying method of slide according to claim 1,2,3 or 4 described a kind of liquid based cytology diagnosis, it is characterized in that the concentration of glutaraldehyde solution in the described step (3) is 4～8%.
8. according to the modifying method of the described a kind of liquid based cytology diagnosis of claim 5, it is characterized in that the concentration of glutaraldehyde solution in the described step (3) is 4～8% with slide.
9. according to the modifying method of the described a kind of liquid based cytology diagnosis of claim 6, it is characterized in that the concentration of glutaraldehyde solution in the described step (3) is 4～8% with slide.
10. use the modifying method of slide according to claim 1,2,3 or 4 described a kind of liquid based cytology diagnosis, it is characterized in that the time that slide reacts in the described step (3) is 20～40 minutes in glutaraldehyde solution, temperature of reaction is 18～30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010265545 CN101955320A (en) | 2010-08-30 | 2010-08-30 | Method for modifying slide for liquid-based cytodiagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010265545 CN101955320A (en) | 2010-08-30 | 2010-08-30 | Method for modifying slide for liquid-based cytodiagnosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101955320A true CN101955320A (en) | 2011-01-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010265545 Pending CN101955320A (en) | 2010-08-30 | 2010-08-30 | Method for modifying slide for liquid-based cytodiagnosis |
Country Status (1)
| Country | Link |
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| CN (1) | CN101955320A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324291A (en) * | 2021-12-27 | 2022-04-12 | 浙江工业大学 | Photocrosslinked molecular probe immobilized on special glass slide, preparation method and application thereof |
Citations (6)
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| CN1425920A (en) * | 2002-12-26 | 2003-06-25 | 浙江大学 | Method for fixing biological macro molecule in common pattern on inorganic silicone material surface |
| CN1619310A (en) * | 2004-11-03 | 2005-05-25 | 东南大学 | Preparation and detection method of tumor immune cell detection chip |
| CN1678758A (en) * | 2002-07-27 | 2005-10-05 | 维尔精股份有限公司 | Large circular sense molecule array |
| CN2771864Y (en) * | 2005-03-22 | 2006-04-12 | 山东省医药生物技术研究中心 | Protein chip for detecting blood cerebrospinal fluid pathogenic antibody |
| CN101065665A (en) * | 2004-11-24 | 2007-10-31 | 康宁股份有限公司 | Polymer-coated substrates for binding biomolecules and methods of making and using thereof |
| CN101371139A (en) * | 2005-12-29 | 2009-02-18 | 康宁股份有限公司 | Supports for assaying analytes and methods of making and using thereof |
-
2010
- 2010-08-30 CN CN 201010265545 patent/CN101955320A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1678758A (en) * | 2002-07-27 | 2005-10-05 | 维尔精股份有限公司 | Large circular sense molecule array |
| CN1425920A (en) * | 2002-12-26 | 2003-06-25 | 浙江大学 | Method for fixing biological macro molecule in common pattern on inorganic silicone material surface |
| CN1619310A (en) * | 2004-11-03 | 2005-05-25 | 东南大学 | Preparation and detection method of tumor immune cell detection chip |
| CN101065665A (en) * | 2004-11-24 | 2007-10-31 | 康宁股份有限公司 | Polymer-coated substrates for binding biomolecules and methods of making and using thereof |
| CN2771864Y (en) * | 2005-03-22 | 2006-04-12 | 山东省医药生物技术研究中心 | Protein chip for detecting blood cerebrospinal fluid pathogenic antibody |
| CN101371139A (en) * | 2005-12-29 | 2009-02-18 | 康宁股份有限公司 | Supports for assaying analytes and methods of making and using thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324291A (en) * | 2021-12-27 | 2022-04-12 | 浙江工业大学 | Photocrosslinked molecular probe immobilized on special glass slide, preparation method and application thereof |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20110126 |