CN101940960B - Anti-back-suction electronic pipettor - Google Patents
Anti-back-suction electronic pipettor Download PDFInfo
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- CN101940960B CN101940960B CN2010102759916A CN201010275991A CN101940960B CN 101940960 B CN101940960 B CN 101940960B CN 2010102759916 A CN2010102759916 A CN 2010102759916A CN 201010275991 A CN201010275991 A CN 201010275991A CN 101940960 B CN101940960 B CN 101940960B
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- pipette
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- monitoring module
- level monitoring
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- 239000007788 liquid Substances 0.000 claims abstract description 84
- 238000012544 monitoring process Methods 0.000 claims abstract description 74
- 239000002904 solvent Substances 0.000 abstract description 4
- 238000012864 cross contamination Methods 0.000 abstract 1
- 238000005213 imbibition Methods 0.000 description 42
- 238000010079 rubber tapping Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 3
- 238000005452 bending Methods 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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Abstract
一种防倒吸电子移液器，包括移液管、安装移液管的移液管接头，还包括并联的正负压泵、吸液开关和放液开关，用于监测移液管上预定监测平面并生成相应的监测信号的液位监测模块，并联的吸液开关与正负压泵构成吸液控制回路，放液开关和正负压泵构成放液控制回路，所述液位监测模块与所述吸液开关相连，根据液位监测模块的相应的监测信号控制吸液开关状态。上述防倒吸电子移液器，采用液位监测模块监测移液管上设置的预定监测平面，根据移液管内液体是否到达预定监测平面处，生成相应的监测信号，根据相应的监测信号控制吸液开关的状态，有效的防止倒吸的发生，防止了溶剂交叉污染。
An anti-suckback electronic pipette, including a pipette, a pipette joint for installing the pipette, and a parallel positive and negative pressure pump, a suction switch and a liquid discharge switch, used to monitor the predetermined pressure on the pipette. A liquid level monitoring module that monitors the plane and generates corresponding monitoring signals. The liquid suction switch and the positive and negative pressure pumps in parallel form a liquid suction control circuit, and the liquid discharge switch and the positive and negative pressure pumps form a liquid discharge control circuit. The liquid level monitoring module and The liquid suction switch is connected, and the state of the liquid suction switch is controlled according to the corresponding monitoring signal of the liquid level monitoring module. The above anti-suckback electronic pipette uses a liquid level monitoring module to monitor the predetermined monitoring plane set on the pipette, generates a corresponding monitoring signal according to whether the liquid in the pipette reaches the predetermined monitoring plane, and controls the suction according to the corresponding monitoring signal. The status of the liquid switch can effectively prevent the occurrence of sucking back and prevent cross-contamination of solvents.
Description
[technical field]
The present invention relates to pipettor, particularly a kind of anti-suck electronic liquid trasferring device.
[background technology]
Electronic liquid trasferring device is as the breadboard liquid instrument that mainly moves, and auxiliary pipette sucks or discharge liquid.Electronic liquid trasferring device in use if suck excessive liquid, exceeds the volume of pipette, and excess liquid will get in the pipettor, and suck-back takes place.Suck-back occurs in operation for a long time, neophyty operation and cell manipulation easily.Suck-back takes place occur harmful effect easily, like the risk of experiment failure, sample cross pollution, biological diffusion, the service life of shortening pipettor etc.
Traditional anti-suck method mainly contains two kinds, and a kind of is to install the cotton filtering plug additional in the pipette upper end, but weak effect can not effectively prevent suck-back; A kind of is behind the pipette joint, to install waterproof filter additional, and the prevention suck-back that this method can not be real can only prevent that liquid from getting into the negative pressure system of pipettor; To alleviate the adverse consequences of suck-back; Therefore problems such as the failure of an experiment, cross pollution can not be effectively solved, and after suck-back takes place, waterproof filter must be changed; Influence the experiment progress, increase experimental cost.
[summary of the invention]
Based on this, be necessary to provide a kind of liquid suck-back that effectively prevents, prevent the anti-suck electronic liquid trasferring device of solution cross pollution.
A kind of anti-suck electronic liquid trasferring device; The pipette joint that comprises pipette, installation pipette; The positive negative pressure pump, imbibition switch and the tapping switch that also comprise parallel connection; Be used to monitor the predetermined level monitoring module of monitoring the plane and generating corresponding monitor signal on the pipette, the imbibition switch of parallel connection constitutes the imbibition control loop with positive negative pressure pump, and tapping switch and positive negative pressure pump constitute the tapping control loop; Said level monitoring module links to each other with said imbibition switch, according to the corresponding monitor signal control imbibition on off state of level monitoring module; Said anti-suck electronic liquid trasferring device also comprises the gating switch of connecting with said imbibition switch, and said level monitoring module links to each other with said gating switch, and controls the break-make of said gating switch according to corresponding monitor signal; When the predetermined monitoring plane of said level monitoring module monitors to said pipette do not have liquid, generate the high level monitor signal, it is closed to control said gating switch, and the imbibition control loop is closed, and the imbibition switch is an effective status; When there is liquid on the predetermined monitoring plane of said level monitoring module monitors to pipette, generate the low level monitor signal, control said gating switch and break off, the imbibition control loop breaks off, and the imbibition switch is a failure state.
Preferably, said gating switch is normally closed relay or NPN type triode switch.
Preferably, said anti-suck electronic liquid trasferring device also comprises the fixed support of fixing said level monitoring module, and said fixed support is located at the lower end of said pipette joint.
Preferably, said fixed support adopts hollow design.
Preferably, said fixed support is arranged on lower end 2 centimeters apart from said pipette joint.
Preferably, said level monitoring module is a liquid level sensor, comprises light-projecting component and photo detector, and said light-projecting component and photo detector are arranged on the both sides of pipette.
Preferably, said light-projecting component is a light emitting diode, and said photo detector is a phototriode.
Preferably, the power supply mode of said liquid level sensor adopts 5～12 volts direct current supply.
Above-mentioned anti-suck electronic liquid trasferring device; Adopt the predetermined monitoring plane that is provided with on the level monitoring module monitors pipette; Whether arrive place, predetermined monitoring plane according to liquid in the pipette, generate corresponding monitor signal, according to the state of corresponding monitor signal control imbibition switch; Effectively prevent the generation of suck-back, prevented the solvent cross pollution.
[description of drawings]
Fig. 1 is the total sketch map of anti-suck electronic liquid trasferring device among the embodiment;
Fig. 2 is the circuit module structural representation of anti-suck electronic liquid trasferring device among the embodiment;
Fig. 3 is the circuit module structural representation of anti-suck electronic liquid trasferring device among another embodiment.
[specific embodiment]
As depicted in figs. 1 and 2, in one embodiment, a kind of anti-suck electronic liquid trasferring device comprises the pipette joint 20 of pipette 10, installation pipette 10, imbibition switch 30, tapping switch 40 and positive negative pressure pump 50, level monitoring module 60 parallel with one another.Wherein, imbibition switch 30 links to each other with level monitoring module 60.Level monitoring module 60 is used to monitor the predetermined monitoring plane on the pipette 10 and generates corresponding monitor signal, and controls the state of imbibition switch 30 according to corresponding monitor signal.The imbibition switch 30 of parallel connection constitutes the imbibition control loop with positive negative pressure pump 50, and tapping switch 40 constitutes the tapping control loop with positive negative pressure pump 50.Should set as required on predetermined monitoring plane, can be at the one circle graduation mark of the somewhere plane picture on the pipette 10 as predetermined monitoring plane.
In the present embodiment, monitor signal is the high-low level signal, and the state of imbibition switch 30 is divided into effective status and failure state.Level monitoring module 60 monitors predetermined monitoring plane on the pipette 10 when not having liquid, generates the high level monitor signal, and control imbibition switch 30 be an effective status, i.e. the 10 continuation imbibitions of imbibition switch 30 controllable pipettes; Level monitoring module 60 monitors predetermined monitoring plane on the pipette 10 when liquid is arranged, and generates the low level monitor signal, and control imbibition switch 30 be a failure state, i.e. imbibition switch 30 inefficacies can not be controlled pipette 10 imbibitions.Through the predetermined monitoring of level monitoring module 60 monitorings plane whether liquid is arranged like this; Generate corresponding monitor signal, the state of control imbibition switch 30 has prevented that imbibition from surpassing the volume of pipette 10 and continuing imbibition generation suck-back; Reached the purpose that effectively prevents suck-back; Prevent the cross pollution of solvent, and need not change the consumptive material of anti-suck, reduce cost.
In one embodiment, above-mentioned anti-suck electronic liquid trasferring device also comprises the gating switch of connecting with imbibition switch 30.Level monitoring module 60 is according to the break-make of monitor signal control gating switch, and then control imbibition switch 30 is effective or failure state.When gating switch was closed, the imbibition control loop was closed, and imbibition switch 30 is an effective status; When gating switch broke off, the imbibition control loop broke off, and imbibition switch 30 is a failure state.Gating switch is normally closed relay 70 or NPN type triode switch.In the present embodiment, gating switch is a normally closed relay 70.According to the closed of monitor signal control normally closed relay 70 or break off, promptly during high level, the control coil two ends power supply of normally closed relay 70, normally closed relay 70 conductings, during low level, the control coil two ends dead electricity of normally closed relay 70, normally closed relay 70 breaks off.Adopt the closed of control normally closed relay 70 or break off the break-make reliability height of realizing the imbibition control loop.
In one embodiment, level monitoring module 60 comprises light-projecting component 600 and photo detector 610.The photoelectric sensor EE-SPX61 liquid level sensor (amplifier embedded conduit mount type) that this Level Detection module 60 adopts Omron Corp to produce, its control is output as NPN open collector output type.The operation principle of this liquid level sensor is to utilize the different of liquid level and air refractive index to come tracer liquid to have or not.Start-up mode when selecting the output action pattern to be shading, then liquid level sensor monitors when in the pipette of locating on predetermined monitoring plane 10 liquid being arranged, and monitor signal promptly switches to low level from high level.Level monitoring module 60 is fixed on pipette joint 20 lower ends through fixed support, and light-projecting component 600 and photo detector 610 are located at the both sides of pipette 10.In the present embodiment; The sign liquid level is overflowed line on the pipette 10; Level monitoring module 60 is fixed on pipette joint 20 lower ends 2 centimeters through fixed support; And guarantee that pipette 10 parts are inserted the predetermined monitoring plane of pipette joint 20 back level monitoring modules 60 and the liquid level of sign is overflowed line at grade, light-projecting component 600 and photo detector 610 are arranged on the both sides of this conplane pipette 10., level monitoring module 60 is fixed on 2 centimeters of pipette joint 20 lower ends here, both can absorbs more liquid, have and reserve 2 centimetres space, prevent that liquid from surpassing the volume generation suck-back of pipette 10.Fixed support adopts hollow design, and the insulated conductor of liquid level sensor is inner through fixed support, link to each other with the control coil of normally closed relay 70, and liquid level sensor adopts 5～12 volts direct current to supply power.Fixed support adopts hollow, and insulated conductor is laid in inside, prevents the lead long term exposure, shortens the life-span, also is prone to cause the inconvenience of operation.
With the gating switch is that normally closed relay 70 is an example; The course of work of anti-suck electronic liquid trasferring device is: when liquid level in the pipette 10 does not reach the predetermined monitoring plane of level monitoring module 60 monitorings, in place, predetermined monitoring plane pipette 10, be air, the light that the light-projecting component 600 of level monitoring module 60 sends can be according to tube bending; Get into photo detector 610 (promptly going into light state); Then generate the output of high level monitor signal, control normally closed relay 70 is in closure state, and the imbibition control loop is closed; Imbibition switch 30 is effective, can continue imbitition; When liquid level in the pipette 10 met or exceeded predetermined monitoring plane, the light that the light-projecting component 600 of level monitoring module 60 sends can not get into photo detector 610 (being the shading state) through behind the liquid refracting; Then generate the output of low level monitor signal; Normally closed relay 70 control coil dead electricity, normally closed relay 70 breaks off immediately, and imbibition switch 30 is in failure state; The imbibition operation can't be proceeded, and has prevented the generation of suck-back operation.This place's gating switch also can adopt NPN type triode switch.
As shown in Figure 3, in one embodiment, the light-projecting component 600 of Level Detection module 60 is a light emitting diode, and photo detector 610 is a phototriode, and in the present embodiment, gating switch is a NPN type triode switch.When place, predetermined monitoring plane was for air in the pipette 10, the light that light emitting diode sends can get in the phototriode according to tube bending, then generates high level signal, and control NPN type triode switch is closed, and the imbibition control loop is closed, can continue imbibition; When liquid level in the pipette 10 meets or exceeds predetermined monitoring plane; The light that light emitting diode sends can not get into phototriode after through liquid refracting, then generates low level signal, and control NPN type triode switch breaks off; The imbibition control loop breaks off, and can't continue imbibition.Adopt the triode switch cost low.Certainly, in the present embodiment, gating switch also can be normally closed relay 70.
Above-mentioned anti-suck electronic liquid trasferring device; Adopt the predetermined monitoring plane that is provided with on the level monitoring module 60 monitoring pipettes 10; Whether arrive place, predetermined monitoring plane according to liquid in the pipette 10, generate monitor signal, according to the state of monitor signal control imbibition switch 30; Effectively prevent the generation of suck-back, prevented the solvent cross pollution.
The above embodiment has only expressed several kinds of embodiments of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art under the prerequisite that does not break away from the present invention's design, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102759916A CN101940960B (en) | 2010-09-07 | 2010-09-07 | Anti-back-suction electronic pipettor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102759916A CN101940960B (en) | 2010-09-07 | 2010-09-07 | Anti-back-suction electronic pipettor |
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| Publication Number | Publication Date |
|---|---|
| CN101940960A CN101940960A (en) | 2011-01-12 |
| CN101940960B true CN101940960B (en) | 2012-11-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102759916A Active CN101940960B (en) | 2010-09-07 | 2010-09-07 | Anti-back-suction electronic pipettor |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106492897A (en) * | 2016-12-28 | 2017-03-15 | 协和华东干细胞基因工程有限公司 | A kind of electric liquid suction device suck-back alarm device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5271902A (en) * | 1990-11-30 | 1993-12-21 | Tosoh Corporation | Quantitative liquid sampling instrument |
| CN101607215A (en) * | 2009-07-08 | 2009-12-23 | 吕志荣 | The calibrating installation and the method for operating thereof that are used for electronic liquid trasferring device |
| CN101795771A (en) * | 2007-09-07 | 2010-08-04 | 艾本德股份有限公司 | Pipette or dispenser with piston position display |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2688163B2 (en) * | 1993-06-04 | 1997-12-08 | アロカ株式会社 | Dispensing device |
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2010
- 2010-09-07 CN CN2010102759916A patent/CN101940960B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5271902A (en) * | 1990-11-30 | 1993-12-21 | Tosoh Corporation | Quantitative liquid sampling instrument |
| CN101795771A (en) * | 2007-09-07 | 2010-08-04 | 艾本德股份有限公司 | Pipette or dispenser with piston position display |
| CN101607215A (en) * | 2009-07-08 | 2009-12-23 | 吕志荣 | The calibrating installation and the method for operating thereof that are used for electronic liquid trasferring device |
Non-Patent Citations (2)
| Title |
|---|
| 图1，2. |
| 实施例1，2. |
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| CN101940960A (en) | 2011-01-12 |
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Effective date of registration: 20230104 Address after: A501, Xinggang Tongchuang Huitianquan Tower, No. 6099, Bao'an Avenue, Qiaotou Community, Fuhai Street, Bao'an District, Shenzhen, Guangdong 518103 Patentee after: SHENZHEN EHPRO TECHNOLOGY CO.,LTD. Address before: 1068 No. 518055 Guangdong city in Shenzhen Province, Nanshan District City Xili University School Avenue Patentee before: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY CHINESE ACADEMY OF SCIENCES |
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