CN101940266B - Mycoprotein nutrient product - Google Patents
Mycoprotein nutrient product Download PDFInfo
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- CN101940266B CN101940266B CN2010102679131A CN201010267913A CN101940266B CN 101940266 B CN101940266 B CN 101940266B CN 2010102679131 A CN2010102679131 A CN 2010102679131A CN 201010267913 A CN201010267913 A CN 201010267913A CN 101940266 B CN101940266 B CN 101940266B
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- acid sodium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention provides a preparation method for a mycoprotein nutrient product which can be used in sunny or rainy whether and at a high or low temperature in an aquaculture breeding process. The method comprises the following steps of: (1) culturing rhodopseudomonas palustris by illumination; (2) flocculating and settling by using 0.01 mol.L<-1> poly aluminum chloride (PAC ) under the condition that pH is 7 to 7.5; concentrating to the effective viable count of over 100 billion CFU/ml and the protein content of over 10 percent; centrifuging to obtain a centrifuge product; and (3) mixing the centrifuge product in the step (2), dusty yeast, p-nitrobenzenesulfonic acid sodium and o-nitrobenzenesulfonic acid sodium in proportion to obtain the product of the invention. 1 ton product comprises 10 percent of the dusty yeast based on the weight of the product, 1kg of the p-nitrobenzenesulfonic acid sodium and 1kg of the o-nitrobenzenesulfonic acid sodium, and the balance of the centrifuge product obtained in the step (2). The product obtained by the preparation method can provide protein nutrition for breeding aquatic animals and promote algal growth.
Description
Technical field
The invention belongs to aquaculture field, be specifically related to a kind of mycoprotein nutrient product.
Background technology
Aquaculture is artificial control breeding down, cultivate and the activity in production of results aquatic animals and plants, generally comprises under the artificial feeding management overall process of forming aquatic products from seed.Existing mainly is three major types at the used fertilizer of culture fishery kind at present, promptly traditional fertilizer, inorganic fertilizer and bio-feritlizer.Traditional fertilizer has its advantage, but fertilizer efficiency is slow, and the process of being utilized by planktonic organism is longer, and in decomposing mineralization process, consumes a large amount of oxygen in the water body, and this is unfavorable to growths such as fish; Simultaneously, in most of the cases, utilization ratio is low, causes water pollution easily; In addition, organic fish guano should be made base manure, and is mainly used in the Winter-Spring water temperature than low season.Though inorganic fertilizer comes rapidly fertile, function is also comparatively single, uses a series of problems such as quality of bringing environment, water quality and aquatic products in a large number.CN1480029A discloses a kind of organic type efficient guano, and CN1480030A discloses a kind of inorganic type efficient guano, and these two kinds just belong to traditional single type fish guano.Except that the fertilizer and inorganic fertilizer of simple function; From last century the eighties also begin to promote " rational application of fertilizers ", i.e. " composite organic-inorganic fertilizer "; Though composite fertilizer has absorbed the advantage of single fertilizer and inorganic fertilizer two aspects, their shortcoming is not fine to be overcome.CN1238321A discloses a kind of high-performance bio fertilizer formula and manufacturing approach, and the purpose of this product is to satisfy different crops and all kinds of plant desired nutritional composition.
But in the natural conditions, algae is very slow in the growth of low temperature and rainy weather, runs into the rainy weather of continuous low temperature in the aquaculture and can limit the cultivated animals growth of ingesting, reduction culture benefit.That is to say; At low temperature and rainy weather, primary producer's algae grows is suppressed in the water body on the one hand, and various is the aquatic animal feed source minimizing of food with the animals and plants that swim; The aquatic animal desire of ingesting descends on the other hand, has influenced the growth of culturing aquatic animal greatly.Existing research does not all have to this point to be carried out, do not see at present have preferably, at weather weather and all spendable aquatic products of high temperature low temperature with mycoprotein nutrition with promote the algal grown product.
Summary of the invention
The present invention is directed to the weak point of prior art, weather weather and all spendable mycoprotein nutrient product of high temperature low temperature in a kind of aquaculture process are provided, nutrient protein is provided and promotes algal grown for culturing the aquatic organism animal.
Product of the present invention makes through following method:
(1) through the illumination cultivation Rhodopseudomonas palustris;
(2) under the condition of pH7-7.5, use aluminium polychloride (PAC) flocculating setting of concentration as 0.01mol.L-1, be concentrated into living bacteria count greater than 1,000 hundred million CFU/ml, protein content is greater than 10%, and is centrifugal, obtains centrifugal product;
(3) the centrifugal product of blend step (2) and dusty yeast, paranitrobenzenesulfonic acid sodium and o-nitrobenzene sulfonic acid sodium in proportion obtain product of the present invention.Contain the dusty yeast of product weight 10%, paranitrobenzenesulfonic acid sodium and the o-nitrobenzene sulfonic acid sodium of each 1kg in the product per ton, surplus is the centrifugal product that step (2) obtains.
Preferably, the condition of illumination cultivation is: use following culture medium: citric acid 8.1g/L, NH_4Cl 3.5g/L, corn steep liquor 1.8g/L; MgSO_40.12g/L, Na_2HPO_42.05g/L, FeSO_40.03g/L and Na_2CO_32.22g/L; Surplus is a water, at temperature 30-32 ℃, and pH value 6.8-7.5; Under the condition of luminous intensity 2800-3200Lcd, illumination cultivation Rhodopseudomonas palustris, living bacteria count >=1,000,000,000/ml.
Wherein, the Rhodopseudomonas palustris bacterium numbering: 1.2180, generic name: Rhodopseudomonas separatrix :=JCM 2524, depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center.
Preferably, centrifugal product in the product of the present invention (concentrating Rhodopseudomonas palustris) in the step (3): dusty yeast: paranitrobenzenesulfonic acid sodium: the usage ratio of o-nitrobenzene sulfonic acid sodium is 898: 100: 1: 1.
The advantage of technical scheme of the present invention is:
1, this product protein active is high, and Rhodopseudomonas palustris contains 65% protein and other important life active compound such as ubiquinone, B family vitamin, folic acid and biotin etc.
2, this product nutrient protein partly is an active bacteria formulation, can improve function of intestinal canal after absorption aquatic animal body is interior, improves immunity of organisms, promotes to ingest growth.
3, this product low temperature overcast and rainy day weather also can promote algal grown;
4, this product Rhodopseudomonas palustris and paranitrobenzenesulfonic acid sodium and the acting in conjunction of o-nitrobenzene sulfonic acid sodium improve water quality, stablize water pH value, the useful algal grown breeding of directed promotion.
, aquatic animal compares the following advantage of this product tool for providing the product of nutrient protein with other:
1, protein active is high, and Rhodopseudomonas palustris contains 65% protein and other important life active compound such as ubiquinone, B family vitamin, folic acid and biotin etc.
2, this product nutrient protein partly is an active bacteria formulation, can improve function of intestinal canal after absorption aquatic animal body is interior, improves immunity of organisms, promotes to ingest growth.
Promote the algal grown product relatively with other, this product has following advantage:
1, low temperature overcast and rainy day weather also can promote algal grown;
2, Rhodopseudomonas palustris and paranitrobenzenesulfonic acid sodium and the acting in conjunction of o-nitrobenzene sulfonic acid sodium improve water quality, stablize water pH value, the useful algal grown breeding of directed promotion.
Description of drawings
Below in conjunction with accompanying drawing the present invention is further specified.
Fig. 1 is a preparation method's of the present invention process chart.
The specific embodiment
The product that preparation method of the present invention makes has added strong food calling effect of tool and nutritious mycoprotein on the one hand; Nutrition is provided and promotes and ingest; On the other hand; Rhodopseudomonas palustris improves water quality and promotes useful algal grown breeding simultaneously, for aquatic animal provides direct and indirect feed source as a kind of water body probio and unicellular alga auxogen paranitrobenzenesulfonic acid sodium and the acting in conjunction of o-nitrobenzene sulfonic acid sodium.
Embodiment 1:
Use following culture medium: citric acid 8.1g/L, NH_4Cl 3.5g/L, corn steep liquor 1.8g/L; MgSO_40.12g/L, Na_2HPO_42.05g/L, FeSO_40.03g/L and Na_2CO_32.22g/L; Surplus is a water, at temperature 30-32 ℃, and pH value 6.8-7.5; Under the condition of luminous intensity 2800-3200Lcd, illumination cultivation Rhodopseudomonas palustris, living bacteria count >=1,000,000,000/ml; Under the condition of pH7-7.5, use aluminium polychloride (PAC) flocculating setting of concentration as 0.01mol.L-1 then, be concentrated into living bacteria count greater than 1,000 hundred million CFU/ml, protein content is greater than 10%; Dusty yeast and paranitrobenzenesulfonic acid sodium and each 1kg of o-nitrobenzene sulfonic acid sodium of adding 10% in the product of centrifugal back per ton mix then, process these article.
Invention has been described according to specific exemplary embodiment here.It will be conspicuous under not departing from the scope of the present invention, carrying out suitable replacement to one skilled in the art or revise.Exemplary embodiment only is illustrative, rather than to the restriction of scope of the present invention, scope of the present invention is by appended claim definition.
Claims (3)
1. the preparation method of weather weather and all spendable mycoprotein nutrient product of high temperature low temperature in the aquaculture process is characterized in that this method may further comprise the steps:
(1) through illumination cultivation Rhodopseudomonas palustris (Rhodopseudanonas palustris);
(2) under the condition of pH7-7.5, use aluminium polychloride (PAC) flocculating setting of concentration as 0.01mol/L, be concentrated into living bacteria count greater than 1,000 hundred million CFU/ml, protein content is greater than 10%, and is centrifugal, obtains centrifugal product;
(3) the centrifugal product of blend step (2) and dusty yeast, paranitrobenzenesulfonic acid sodium and o-nitrobenzene sulfonic acid sodium in proportion; Obtain the mycoprotein nutrient product; Contain the dusty yeast of product weight 10%, paranitrobenzenesulfonic acid sodium and the o-nitrobenzene sulfonic acid sodium of each 1kg in the product per ton, surplus is the centrifugal product that step (2) obtains.
2. preparation method as claimed in claim 1 is characterized in that: in the step (1), the condition of illumination cultivation is: use following culture medium: citric acid 8.1g/L, NH
4C1 3.5g/L, corn steep liquor 1.8g/L, MgSO
40.12g/L, Na
2HPO
42.05g/L, FeSO
40.03g/L and Na
2CO
32.22g/L surplus is a water, at temperature 30-32 ℃, and pH value 6.8-7.5, under the condition of luminous intensity 2800-3200cd, illumination cultivation Rhodopseudomonas palustris, living bacteria count>=1,000,000,000/ml.
3. weather weather and all spendable mycoprotein nutrient product of high temperature low temperature in the aquaculture process that makes of according to claim 1 or claim 2 preparation method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102679131A CN101940266B (en) | 2010-08-31 | 2010-08-31 | Mycoprotein nutrient product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102679131A CN101940266B (en) | 2010-08-31 | 2010-08-31 | Mycoprotein nutrient product |
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| Publication Number | Publication Date |
|---|---|
| CN101940266A CN101940266A (en) | 2011-01-12 |
| CN101940266B true CN101940266B (en) | 2012-08-22 |
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| CN2010102679131A Expired - Fee Related CN101940266B (en) | 2010-08-31 | 2010-08-31 | Mycoprotein nutrient product |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050446A (en) * | 2007-03-21 | 2007-10-10 | 珠海市农业科学研究中心 | Swamp Rhodopseudomonas of using nitrite nitrogen in high effect, and application |
| CN101595943A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of additive of bait for photosynthetic bacteria aquaculture |
-
2010
- 2010-08-31 CN CN2010102679131A patent/CN101940266B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050446A (en) * | 2007-03-21 | 2007-10-10 | 珠海市农业科学研究中心 | Swamp Rhodopseudomonas of using nitrite nitrogen in high effect, and application |
| CN101595943A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of additive of bait for photosynthetic bacteria aquaculture |
Non-Patent Citations (2)
| Title |
|---|
| 周茂洪.光合细菌及其在养殖业中应用研究进展.《温州大学学报》.2001,(第1期),全文. * |
| 温广明等.光合细菌在水产养殖中的应用.《山西水利科技》.2002,(第2期),全文. * |
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| CN101940266A (en) | 2011-01-12 |
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Effective date of registration: 20150929 Address after: 430000 Hubei city of Wuhan province and Economic Development Zone Jiangxia road Hubei green green engineering development Limited by Share Ltd 1-10 comprehensive building Patentee after: Wuhan full coke feed Technology Co., Ltd. Address before: 430223, East Lake Wuhan hi tech Development Zone, Hubei Province Temple Mountain District green biological engineering Park Patentee before: Wuhan Thrive Biological Technology Co., Ltd. |
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