CN101919406A - A kind of biological fungicide wettable powder - Google Patents
A kind of biological fungicide wettable powder Download PDFInfo
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- CN101919406A CN101919406A CN2009100746853A CN200910074685A CN101919406A CN 101919406 A CN101919406 A CN 101919406A CN 2009100746853 A CN2009100746853 A CN 2009100746853A CN 200910074685 A CN200910074685 A CN 200910074685A CN 101919406 A CN101919406 A CN 101919406A
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- 238000009736 wetting Methods 0.000 claims description 29
- 230000001580 bacterial effect Effects 0.000 claims description 26
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 9
- 239000003899 bactericide agent Substances 0.000 claims description 9
- 244000144987 brood Species 0.000 claims description 9
- 239000004563 wettable powder Substances 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 claims description 5
- 239000002270 dispersing agent Substances 0.000 claims description 5
- 239000005995 Aluminium silicate Substances 0.000 claims description 4
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 4
- 235000012211 aluminium silicate Nutrition 0.000 claims description 4
- 229920005551 calcium lignosulfonate Polymers 0.000 claims description 4
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 claims description 4
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 description 27
- 239000000843 powder Substances 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 240000008067 Cucumis sativus Species 0.000 description 8
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 8
- 238000001035 drying Methods 0.000 description 8
- 241000221785 Erysiphales Species 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 238000003556 assay Methods 0.000 description 5
- 230000003902 lesion Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000220317 Rosa Species 0.000 description 4
- 241001655322 Streptomycetales Species 0.000 description 4
- 230000002051 biphasic effect Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000008233 hard water Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000187438 Streptomyces fradiae Species 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 229930000044 secondary metabolite Natural products 0.000 description 3
- 238000007873 sieving Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000189524 Baccharis halimifolia Species 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000187181 Streptomyces scabiei Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种生物杀菌剂可湿性粉剂，其组份及含量为：菌株0116繁殖体30-80％；载体40-70％；润湿分散剂2-10％。确定了白炭黑等五种载体和木质素磺酸钠等五种润湿分散剂。而且，采用定期检测活菌数测定菌株0116孢子粉储存稳定性，结果表明0116菌株孢子粉18℃贮存18个月活菌率＞85％。本发明申请具有开发周期短、成本低、工艺简单且可开发为多种剂型的特点，能够进行规模化生产。由于用菌株0116繁殖体作为可湿性粉剂的主要有效成分，能够进行规模化生产，而相较于菌株0116的发酵液来说大大缩小了田间的单位用量，大大降低了包装和运输成本。The invention discloses a biological fungicide wettable powder, which has the following components and content: 30-80% of propagule of bacterial strain 0116; 40-70% of carrier; 2-10% of wetting and dispersing agent. Five kinds of carriers such as white carbon black and five kinds of wetting and dispersing agents such as sodium lignosulfonate were determined. Moreover, the storage stability of the strain 0116 spore powder was measured by regularly detecting the number of viable bacteria, and the results showed that the spore powder of the 0116 strain was stored at 18° C. for 18 months and the viable bacteria rate was > 85%. The application of the present invention has the characteristics of short development cycle, low cost, simple process and can be developed into various dosage forms, and can be produced in a large scale. Since the strain 0116 propagule is used as the main active ingredient of the wettable powder, large-scale production can be carried out, and compared with the fermentation liquid of the strain 0116, the unit dosage in the field is greatly reduced, and the packaging and transportation costs are greatly reduced.
Description
Technical field:
The present invention relates to a kind of wettable powder of biological bactericide of controlling plant diseases.
Background technology
At present, the control of plant disease is mainly based on chemical pesticide, and that the long-term use of chemical pesticide causes the selection pressure of pathogen is big, and pathogen is easy to generate pesticide resistance.Simultaneously the high residue of chemical pesticide, high pollution cause the safety of environment and people, animal and seriously influence.Therefore the biopesticide of low toxicity, low-residual becomes the focus of development.Agricultural University Of Hebei has applied for " a kind of biological bactericide " on November 2nd, 2005, number of patent application 200510117421.3, its component and content are: the mutagenic strain 0116 bacterial strain fermentation liquor 93---96% of rose yellow streptomycete (Streptomyces roseoflavus); Phenethyl phenol polyethenoxy ether 3---5%; Sodium acetate (CH
3COONa) .5---1%; Acetic acid (CH
3COOH) .3---1%.This novel bacterial entrusts that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " carries out preservation, strain name: rose yellow streptomycete 0116, deposit number: CGMCC N0.1471.Mutagenic strain 0116 bacterial strain of rose yellow streptomycete (Streptomyces roseoflavus) is hereinafter to be referred as bacterial strain 0116, it is the bacterial strain that obtains through a strain rose yellow streptomycete (Streptomyces roseoflavus) mutagenesis, the zymotic fluid of its viable bacteria has inhibitory action to multiple important phytopathogen, for example cotton verticillium wilt pathogen, deep scab of potato pathogen, root rotof flax pathogen, graw mold of tomato pathogen, powdery mildew of cucumber pathogen etc.Greenhouse and field experiment show that all 0116 bacterial strain fermentation liquor has the better prevention effect to cotton verticillium wilt.In recent years, diseases and pests of agronomic crop biological and ecological methods to prevent plant disease, pests, and erosion Engineering Technical Research Centre in Hebei province's is applied to the zymotic fluid of this bacterial strain again in the control to wheat powdery mildew, powdery mildew of cucumber, and all shows good effect.The defective that above-mentioned patent application exists is: at first, the microorganism secondary metabolite is applied as agricultural chemicals or its lead compound, need the clearly accurate structure of this secondary metabolite, what play a major role in 0116 bacterial strain fermentation liquor is the secondary metabolite of bacterial strain, and to the component of zymotic fluid separate, purifying, structure appraisal cost are big, the cycle is longer.So can't chemosynthesis, can not carry out large-scale production.Secondly because the main component of zymotic fluid is a water, so zymotic fluid to directly apply to the unit consumption in field big, cause packing and cost of transportation big.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, the unit consumption that provides a kind of and can carry out large-scale production, is applied to the field is little, packing and the less wettable powder of biological bactericide of cost of transportation.
The objective of the invention is to realize with following technical scheme: a kind of wettable powder of biological bactericide, its component and content are: bacterial strain 0116 brood body 30---80%; Carrier 40---70%; Wetting dispersing agent 2---10%.
Described bacterial strain 0116 brood body is the mycelia and the spore of bacterial strain 0116.
Described carrier is any in white carbon, kaolin, diatomite, talcum powder, the calcium carbonate.
Described wetting dispersing agent is one or more the mixture in calcium lignosulfonate, sodium lignin sulfonate, dodecyl sodium sulfate, neopelex, the lauryl sodium sulfate.
The present patent application have the construction cycle short, cost is low, technology is simple and can be developed as the characteristics of multiple formulation, can carry out large-scale production.Research work shows that the live body of bacterial strain 0116 is fairly obvious to the inhibitory action of the phytopathy original, and bacterial strain is stronger to the anti-adversity ability of environment, and the required nutritional condition of growth and breeding is lower.Its key of the exploitation of active bacteria formulation is that the storage cycle of viable bacteria will reach 1-2, and selects not influence the carrier and the auxiliary agent of thalline activity and storage stability.By plate assay various carriers and auxiliary agent are screened, determined that white carbon, diatomite, kaolin, talcum powder and five kinds of carriers of calcium carbonate and sodium lignin sulfonate, calcium lignosulfonate, dodecyl sodium sulfate, neopelex and five kinds of wetting dispersing agents of lauryl sodium sulfate and bacterial strain 0116 compatibility are better, and, adopt and regularly detect viable count mensuration bacterial strain 0116 conidial powder storage stability, the result shows 18 months viable bacteria rate＞85% of 18 ℃ of storages of 0116 bacterial strain conidial powder.Owing to,, greatly reduce packing and cost of transportation so compared to the zymotic fluid of bacterial strain 0116, dwindled the unit consumption in field greatly with the main active ingredient of bacterial strain 0116 brood body as wetting powder.
For the practicality of patent application of the present invention is described, existing check, assay method and data with patent application sample of the present invention disclose as follows:
The assay method of suspensibility
Take by weighing an amount of sample m
1, be accurate to 0.0001g.Place the 200mL beaker that fills 50mL (30 ± 1 ℃) standard hard water, make circular motion with hand swinging, about per minute 120 times, carry out 2min, this suspension is placed 13min in the water-bath of same temperature, use 30 ± 1 ℃ standard hard water that it is all washed in the 250mL graduated cylinder then, and be diluted to scale, cover stopper, put upside down mixing.Put into vibrationless thermostat water bath again, place 30min, 9/10 suspension of content is shifted out in 10～15s with suction pipe, do not shake or stir the interior precipitum of graduated cylinder, the top of guaranteeing suction pipe is several millimeters places under liquid level always.Precipitum is all washed out the postlyophilization m that weighs
2
Total suspensibility=10/9x[(m
1-m
2)/m
1] x100%.
Take by weighing each 10mg of sample and precipitum, detect its viable count n
1And n
2
Spore suspensibility=10/9x[(m
1n
1-m
2n
2)/m
1n
1] x 100%.
Total suspensibility＞80% of patent application sample of the present invention, spore suspensibility＞90%.
The assay method of wetting time
Get standard hard water 100 ± 1ml, inject the 250ml beaker, this beaker is placed 25 ± 1 ℃ water bath with thermostatic control, make its liquid level concordant with the horizontal plane of water-bath.Take by weighing 5 ± 0.1g sample with surface plate when treating hard water to 25 ± 1 ℃, with whole samples from once being poured over equably on the liquid level of this beaker with beaker mouth flush position, but disturbance liquid level excessively not.Clock with stopwatch immediately when adding sample, till sample is all wetting.Write down wetting time.So repeat five times, get its mean value, as the wetting time of this sample.Moistening time＜the 60s of patent application sample of the present invention.
The assay method of fineness
Take by weighing 20g sample (being accurate to 0.1g), place the 250mL beaker, add about 80mL running water, stir, make its complete wetting with glass bar.Testing sieve is immersed in the water, make the wire cloth complete wetting.With running water sample wetting in the beaker is diluted to about 150mL, stirs, all pour in the wetting standard screen then, wash beaker with running water, washings are also poured in the sieve, till coarse granule moves in the sieve fully in beaker.With diameter is the sample on the current washing screen from the beginning gently that the rubber tube of 9～10mm is derived, and it is concordant with the sieve edge for spending that water velocity control 4～5L/min, the terminal delivery port of rubber tube keep.In sieving and washing process, keep current to aim at sample on the sieve, it is fully washed, wash always by the water of testing sieve limpid transparent till.Again testing sieve is moved in the basin that fills running water, move up and down washing sieve edge and remain on the water surface, be repeated to till no material sieves in the 2min.Discard sieving, residue in will sieving, subpunch to an angle transfers in the 100mL beaker of constant weight.Leave standstill, treat that particles settling is to the bottom in the beaker, most of water that inclines heats, and the residue evaporation is near dried, dries to constant weight in 100 ℃ of baking ovens, takes out beaker and places drier to be cooled to room temperature, weighs.The fineness of patent application sample of the present invention＞98% is by 74 μ m sieve.
X(％)＝(m
1-m
2)×100/m
1
In the formula: m
1The quality of-sample; m
2The quality of residue in the-glass dish (or beaker).
Storage stability test
Adopt the method that regularly is coated with the dull and stereotyped cfu of mensuration value, patent application sample of the present invention unit spore content in 12 months all is stabilized in 10
8More than/the g.
Measured the control efficiency of 0116 viable bacteria wetting powder to powdery mildew of cucumber under condition of living body, concrete grammar and result are as follows:
1. test method:
After preparation was diluted to 10mg/mL, 5mg/mL, 2.5mg/mL, 1.25mg/mL, 1.0mg/mL respectively, it is positive to running off that suspension evenly is sprayed on the cucumber cotyledons sheet, contrast spray clear water, and every concentration is established 6 repetitions (12 young plant).After soup dries 24h, with the fresh conidium of the powdery mildew on the cucumber leaves down, after filtering, double gauze makes conidial suspension (10 with the deionized water brush
5Conidium/mL), with YW5.2-A type micro-sprayer conidial suspension evenly is sprayed on the positive extremely loss of cucumber cotyledons sheet then, inoculation back natural air drying, place plastic casing (87 * 47 * 27cm), add low amounts of water (1cm is dark) in the box, the lid plastic film, the 24h that preserves moisture under 16～20 ℃ of dark conditions moves to and cultivates the morbidity of 7～10d " Invest, Then Investigate " under the greenhouse.
0 grade: anosis;
1 grade: lesion area accounts for the whole blade area below 5% on every blade;
3 grades: lesion area accounts for whole blade area 6%-10% on every blade;
5 grades: lesion area accounts for whole blade area 26%-50% on every blade;
7 grades: lesion area accounts for the whole blade area more than 50% on every blade;
9 grades: lesion area accounts for whole blade area 100% on every blade.
Calculate disease index, press disease index and calculate relative control effect.
Disease index=[∑ (the sick number of sheets * relative progression)/(investigating total number of sheets * total progression)] * 100.
Control efficiency (%)=[(CK disease index-PT disease index)/CK disease index] * 100.
2. result of the test:
0116 viable bacteria wetting powder has good control efficiency to powdery mildew of cucumber, its EC
50Value is 2.0mg/mL.5.0mg/mL preventive effect reach 82%, result of the test sees Table 1.
Table 10116 viable bacteria wetting powder is to the preventive effect of powdery mildew of cucumber
Embodiment
Embodiment 1, prepares 60%0116 viable bacteria wetting powders.
The 0116 strain fermentation product that liquid-solid biphasic fermentation is obtained is in vacuum cooling drying 24h, pulverized 200 mesh sieves then, promptly get the powder of 0116 bacterial strain brood body, get this powder 6.0g, white carbon 3.0g, lauryl sodium sulfate 1.0g abundant stirring and evenly mixing in homogenizer obtains 60%0116 viable bacteria wetting powders.
Bacteria containing amount (cfu/g) 〉=10
8, viable bacteria rate (%) 〉=90,
Loss on drying (%)≤3.5, suspensibility (%)＞80,
Wetting time (s)＜60s, fineness (by 74 μ m sieve)＞98%,
PH value: 5-8, bin stability: store 12 months viable bacteria rate＞85% for 18 ℃.
Embodiment 2, prepare 50%0116 viable bacteria wetting powders.
The 0116 strain fermentation product that liquid-solid biphasic fermentation is obtained was pulverized 200 mesh sieves then in vacuum cooling drying 24h, promptly got the powder of 0116 bacterial strain brood body.Get this powder 5.0g, diatomite 4.4g, sodium lignin sulfonate 0.6g abundant stirring and evenly mixing in homogenizer obtains 50%0116 viable bacteria wetting powders.
Bacteria containing amount (cfu/g) 〉=10
8, viable bacteria rate (%) 〉=90,
Loss on drying (%)≤3.5, suspensibility (%)＞80,
Wetting time (s)＜60s, fineness (by 74 μ m sieve)＞98%,
PH value: 5-8, bin stability: store 12 months viable bacteria rate＞85% for 18 ℃.
Example 3 prepares 45%0116 viable bacteria wetting powders.
The 0116 strain fermentation product that liquid-solid biphasic fermentation is obtained was pulverized 200 mesh sieves then in vacuum cooling drying 24h, promptly got the powder of 0116 bacterial strain brood body.Get this powder 4.5g, diatomite 5.0g, 1: 1 mixture 0.5g of sodium lignin sulfonate and calcium lignosulfonate abundant stirring and evenly mixing in homogenizer obtains 45%0116 viable bacteria wetting powders.
Bacteria containing amount (cfu/g) 〉=10
8, viable bacteria rate (%) 〉=90,
Loss on drying (%)≤3.5, suspensibility (%)＞80,
Wetting time (s)＜60s, fineness (by 74 μ m sieve)＞98%,
PH value: 5-8, bin stability: store 12 months viable bacteria rate＞85% for 18 ℃.
Example 4 prepares 35%0116 viable bacteria wetting powders.
The 0116 strain fermentation product that liquid-solid biphasic fermentation is obtained is in vacuum cooling drying 24h, pulverized 200 mesh sieves then, promptly get the powder of 0116 bacterial strain brood body, get this powder 3.5g, kaolin 5.7g, 1: 1 mixture 0.8g of dodecyl sodium sulfate and neopelex abundant stirring and evenly mixing in homogenizer obtains 35%0116 viable bacteria wetting powders.
Bacteria containing amount (cfu/g) 〉=10
8, viable bacteria rate (%) 〉=90,
Loss on drying (%)≤3.5, suspensibility (%)＞80,
Wetting time (s)＜60s, fineness (by 74 μ m sieve)＞98%,
PH value: 5-8, bin stability: store 12 months viable bacteria rate＞85% for 18 ℃.
Claims (4)
1. a wettable powder of biological bactericide is characterized in that, its component and content are: bacterial strain 0116 brood body 30---80%; Carrier 40---70%; Wetting dispersing agent 2---10%.
2. a kind of wettable powder of biological bactericide according to claim 1 is characterized in that, bacterial strain 0116 brood body is the mycelia and the spore of bacterial strain 0116.
3. a kind of wettable powder of biological bactericide according to claim 1 is characterized in that, described carrier is any in white carbon, kaolin, diatomite, talcum powder, the calcium carbonate.
4. a kind of wettable powder of biological bactericide according to claim 1, it is characterized in that described wetting dispersing agent is one or more the mixture in calcium lignosulfonate, sodium lignin sulfonate, dodecyl sodium sulfate, neopelex, the lauryl sodium sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100746853A CN101919406A (en) | 2009-06-09 | 2009-06-09 | A kind of biological fungicide wettable powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100746853A CN101919406A (en) | 2009-06-09 | 2009-06-09 | A kind of biological fungicide wettable powder |
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| Publication Number | Publication Date |
|---|---|
| CN101919406A true CN101919406A (en) | 2010-12-22 |
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ID=43334850
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125527A (en) * | 2011-11-22 | 2013-06-05 | 河北农业大学 | Wettable powder of streptomyces roseoflavus biological bactericide |
| CN104542723A (en) * | 2015-02-09 | 2015-04-29 | 江苏瑞东农药有限公司 | Wettable powder compounded from penoxsulam and biological herbicide and preparation method of wettable powder |
| CN105016838A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Biocontrol bacterium nutritional tablet |
| CN110122484A (en) * | 2019-05-22 | 2019-08-16 | 中国农业科学院植物保护研究所 | Powder-type rose yellow streptomycete NKZ-259 and its to tomato growth-promoting method |
| CN112889842A (en) * | 2021-01-22 | 2021-06-04 | 中国烟草中南农业试验站 | Wettable bacterium powder and preparation method and application thereof |
| CN113248945A (en) * | 2021-05-20 | 2021-08-13 | 杭州君一新材料科技有限公司 | Treatment process of easy-to-disperse special carbon black |
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| CN1265832A (en) * | 1999-11-29 | 2000-09-13 | 解俊峰 | Calcium peroxide wettable powder |
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| CN1262040A (en) * | 1999-01-26 | 2000-08-09 | 遵义市务凤农药分装厂 | Wettable powdered disinfectant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125527A (en) * | 2011-11-22 | 2013-06-05 | 河北农业大学 | Wettable powder of streptomyces roseoflavus biological bactericide |
| CN105016838A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Biocontrol bacterium nutritional tablet |
| CN104542723A (en) * | 2015-02-09 | 2015-04-29 | 江苏瑞东农药有限公司 | Wettable powder compounded from penoxsulam and biological herbicide and preparation method of wettable powder |
| CN110122484A (en) * | 2019-05-22 | 2019-08-16 | 中国农业科学院植物保护研究所 | Powder-type rose yellow streptomycete NKZ-259 and its to tomato growth-promoting method |
| CN112889842A (en) * | 2021-01-22 | 2021-06-04 | 中国烟草中南农业试验站 | Wettable bacterium powder and preparation method and application thereof |
| CN113248945A (en) * | 2021-05-20 | 2021-08-13 | 杭州君一新材料科技有限公司 | Treatment process of easy-to-disperse special carbon black |
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