CN101914555A - Novel genes encoding novel proteolytic enzymes - Google Patents

Abstract

The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

Description

The new gene of encoding novel proteolytic enzymes

Invention field

The present invention relates to new identified polynucleotides sequence, this sequence comprises the gene of coding isolating new proteolytic enzyme from aspergillus niger (Aspergillus niger).The invention describes the full length nucleotide sequence of new gene, the cDNA sequence of complete encoding sequence that comprises new proteolytic enzyme and aminoacid sequence and the fragment and the variant of total length functional protein.The present invention also relates in commercial run, use the method for these enzymes and the method for diagnosis fungi infestation.Comprise equally in the present invention be to use according to polynucleotide cell transformed of the present invention and proteolytic enzyme according to the present invention therein by genetic modification to strengthen or to reduce the cell of its activity and/or expression level.

Background of invention

Proteolytic ferment

Protein can be considered as making up the heteropolymer that piece is formed by the amino acid that connects with peptide bond.Repeating unit in the protein is the center alpha-carbon atom with amino group and carboxylic group.Except that glycine, so-called amino acid side chain has substituted in two remaining α hydrocarbon atoms.Amino acid side chain makes that the center alpha-carbon atom is asymmetric.What usually, find in protein is amino acid whose L-enantiomer.Below term description polytype polyamino acid.Peptide is the amino-acid residue short chain with definite sequence.Although in fact the number for residue does not have maximum value, this term indicates its character mainly to form the chain of determining and not having the fixed three-dimensional conformation by its amino acid usually.The term polypeptide is generally used for longer chain, has definite sequence and length and have suitable length in principle to be folded into three-dimensional structure usually.Protein refers to natural existence and shows the polypeptide of determining three-dimensional structure.If protein is mainly brought into play the function of catalyzed chemical reaction, it is commonly called enzyme so.Proteolytic enzyme is the enzyme of peptide bond hydrolysis in catalysis (many) peptides and the protein.

Under physiological conditions, the hydrolysis of proteolytic enzyme catalysis peptide bond.International biological chemistry and molecular biology association (International Union of Biochemistry andMolecular Biology) (1984) recommend to use the term peptase for peptide bond hydrolysis enzyme hypotype (subclass E.C3.4.).Term protease and peptidohydrolase and peptase be synonym and also can be used for herein.Proteolytic enzyme comprises 2 fermentoids: endopeptidase and exopeptidase, they are the site cutting peptide bonds in protein and continuously from the terminal amino acid of removing of N or C-respectively.Proteolytic enzyme is as the synonym of endopeptidase.Peptide bond can betide under 2-, 3-, 4-peptide, peptide, polypeptide or the proteinic situation.Usually, the amino acid of native peptides and polypeptide is formed and is comprised 20 different amino acid, and this amino acid is showed L-configuration (except that the glycine that does not have chiral centre).Yet the proteolytic activity of proteolytic enzyme is not limited to the peptide that only contains 20 natural amino acids.Also can be cut at the peptide bond between the so-called alpha-non-natural amino acid and at amino acid or the peptide bond between the amino acid analogue modified.Some proteolytic enzyme are accepted the amino acid whose D-conformation of some position really.Usually, the significant stereoselectivity of proteolytic enzyme makes that they are very useful in the process of decomposition.Many proteolytic enzyme are showed interesting secondary activity, as esterase activity, thiolesterase activity and (taking off) lactamase activity.These secondary activity not only are confined to amino acid usually, and susceptible of proof is to be very useful in the bio-transformation in fine chemical field.

Filamentous fungus, eukaryotic microorganisms have special importance many reasons are arranged.If control inadequately, the primary process cost of peptide bond hydrolysis cutting is very big in the protein so, and potential harmful to biology.To proteolysed required restriction be by proteolytic enzyme specificity, by make proteolytic enzyme and substrate intracellular compartmentation, by substrate is modified so that it can be by discrete proteolytic enzyme identification, by by the proenzyme activation with special inhibitor exists or non-existent adjusting and realize by the adjusting that proteinase gene is expressed.In fungi, proteolytic enzyme also relates to other basic cell processes, and this process comprises intracellular protein renewal, processing, transposition, sporulation, sprouting and differentiation.In fact, with Aspergillus nidulans (Aspergillus nidulans) and Neuraspora crassa (Neurospora crassa) model animals as the molecular basis of analytical physiology and growth course scope.Their hereditary property makes and can directly enter biological chemistry and genetics research under nutrient of determining and culture condition.In addition, separated making the morbific fungi of people, livestock and farm crop in a large number, and thought that proteolysis works in their pathogenic (host invades, antagonism host's defense mechanism and/or nutrition in infection).Proteolytic enzyme is also in being usually used in experiment, clinical and commercial run; Microorganism and amicrobic proteolytic enzyme all are widely used in also in the manufacturing of visible foodstuffs industry (cure, brewage, cheese producing, tenderization), tanning industry and bio-decontaminated agent (Aunstrup, 1980).Develop some filamentous fungus, especially aspergillus niger belong to commercial significance as homology or both production hosts of heterologous protein make recently the importance of fungal proteinase recovered (van Brunt, 1986ab).Proteolytic enzyme often causes problem in the protein heterogenous expression of fungi and homology overexpression.Particularly, heterogenous expression is subjected to the obstruction of homologous protein enzyme to the proteolytic degradation of expression product.These commercial benefitss cause studying in great detail that proteolysis spectrum and proteolytic enzyme defective strain make up, and have improved the knowledge of proteolytic enzyme expression and adjusting in relevant these biologies.Just be sought after in filamentous fungus, identifying and eliminating new proteolytic enzyme subsequently.

Microorganism such as fungi are particularly useful in large-scale protein matter is produced.Especially when this protein secreting is in substratum.Proteolytic ferment works in these production processes.On the one hand, concrete usually proteolytic ferment is essential for the correct processing of target protein and production host's metabolism health.On the other hand, proteolytic degradation can significantly reduce the proteinic output of excretory.Bad folding can causing in Secretory Pathway by the intracellular protein enzyme liberating.This may be a special problem to producing heterologous protein.The details of proteolysis process and not exclusively known, this process are responsible for the protein of degrading and shifting from the fungus secretion process.In eukaryote, the degraded of cell protein is realized by proteasome, and is usually directed to the protein that will degrade is carried out the ubiquitin mark.Proteasome also may carry out proteolytic degradation to bad folding secretory protein with the vacuole protein enzyme in fungi.This proteolytic degradation may be in kytoplasm, but can not get rid of the proteolytic enzyme that resides in endoplasmic reticulum.From producing the improved aspect of host's strain, the proteolysis system can be genetically engineered and the improved interesting target of production strain.The additional copy of proteinase gene, the overexpression of some proteolytic enzyme, the gene knockout program of transcribing the modification of control and proteinase gene being lacked can provide more detailed understanding to the function of given proteolytic enzyme.In order to improve the output of homology and heterologous protein, the disappearance of protease-encoding gene is the improved valuable strategy of host's strain.

Eukaryotic microorganisms proteolytic enzyme is summarized by North (1982).More closely, Suarez Rendueles and Wolf (1988) have summarized yeast saccharomyces cerevisiae (S.cerevisiae) proteolytic enzyme and function thereof.

Except that the hydrolysis cutting to key, proteolytic enzyme also can be applicable in the formation of key.The key here comprises that not only peptide and amido linkage also comprise ester bond.Proteolytic enzyme is the cutting of catalysis particular key or forms the thermodynamics that at first depends on this reaction really.Enzyme such as proteolytic enzyme do not influence molecular balance.This balance depends on the special condition that reaction takes place.Under physiological conditions, the thermodynamics of reaction helps the hydrolysis of peptide owing to the zwitterion highly stable structure on thermodynamics that produces.To influence balance, perhaps, perhaps, proteolytic enzyme may be used for peptide bond synthetic purpose by Applied Physics-principles of chemistry by utilizing the kinetic parameter of enzyme reaction by the concentration or the characteristic of control response thing and product.The adding of water mixed organic solvents has reduced the degree of ionization of carboxyl composition, has increased the concentration of reaction available substrate whereby.Often adopt the amino and the carboxylic group of biphasic system, water stand-in (water mimetics), oppositely micelle (reverse micelles), anhydrous medium or modification to increase output with the precipitation that causes product.When the proteolytic enzyme with correct character can utilize, the application of this proteolytic enzyme in synthetic provided the advantage of essence.Because proteolytic enzyme is stereoselectivity and regioselectivity, so the sensitive group on the reactant need not protected usually, and reactant is not required to be optically pure.Because enzymatic synthetic condition is gentle, so can avoid the racemization and the decomposition of unsettled reactant or product.The key between amino acid, other compounds of showing primary amino group, mercapto groups or carboxylic group also can be connected by the proteolytic enzyme of suitable selection.In addition, ester, thiol esters and acid amides can be synthesized by some proteolytic enzyme.Shown proteolytic enzyme display area selectivity in the acylation of list, two-and three-sugar, nucleosides and riboflavin.The problem of stability can be prevented by appropriate formulations under harsh reaction conditions sometimes.Encapsulated and the fixing enzyme of not only having stablized, and make and from reaction culture medium, to reclaim and separates facility.Crosslinked widely, handle or with some polymer such as dextran, polyoxyethylene glycol, poly-imines (polyimines) covering surfaces life-span of extensible biological catalyst in essence with aldehyde.

The natural action of proteolytic enzyme

Traditionally, proteolytic enzyme is regarded as degrading enzyme, and this endonuclease capable is cut into little peptide and/or amino acid with protein, and its renewal that act as digestion nutrient protein or participate in cell protein.In addition, shown that proteolytic enzyme is also playing a key role in the cell processes widely by the mechanism of carrying out selective modification with limited proteolysis, and thereby have basic regulatory function (Holzer and a Tschensche 1979; Holzer and Heinrich, 1980).The specificity of proteolytic enzyme is considered to be closely related with its physiologic function and its expression pattern.As for the function of concrete proteolytic enzyme, its location often is very important; For example, many vacuoles and periplasm protein enzyme participate in protein degradation, and many embrane-associated protein enzymes are in protein processing very important (Suarez Rendueles and Wolf, 1988).The not same-action of the proteolytic enzyme in many cell processes can be divided into 4 main protease functions: 1) protein degradation, 2) (mistakes) activation of translation post-treatment and specified protein, 3) form generation and 4) pathogeny.

Utilizing protein is hydrolysis to nutrient as the obvious effect of proteolytic enzyme in the biology in nutrient source.In fungi, this will comprise the degraded of being carried out in the extracellular by extracellular extensive specific proteolytic enzyme.Protein degradation is also important for the fast updating of cell protein, and makes cell can remove abnormal protein and for its proteinic replenishing of the physiological conditions adaptive change that changes.Normally, avoid degraded at random except that correct target protein, have quite extensive specific proteolytic enzyme and also should carry out extraordinary control in order to protect cell.

Opposite with hydrolysis, the synthetic process that drives by ATP in vivo of polypeptide takes place on rrna.The sequence that final amino acid connects is by being derived from genomic information domination.This process is known as translation.Primary translation product is often long than final function product, and this protein precursor must further be processed by proteolytic enzyme usually after translation.Proteolytic enzyme plays a key role in the final functional protein obtaining in this precursor protein matter maturation.Opposite with reconstruct shape with complete in check protein pruning, proteolytic enzyme also can be very destructive, and polypeptide can be degraded to peptide and amino acid fully.Discharged before needs for fear of proteolytic activity, proteolytic enzyme has been carried out regulation and control widely.Many proteolytic enzyme are that this precursor becomes activatory when needs as the precursor synthetic that is known as proenzyme.This activation always takes place by proteolysis significantly.Except that directly relating to this process, single proteinic selectivity activation and inactivation are well-known by the enzymatic phenomenon of specific protein.

Limited proteoclastic selectivity seems and more directly is present in the interaction of albumen enzyme-substrate.Specificity can be derived from the proteolytic ferment of only discerning the specific amino acids target sequence.On the other hand, it also can be the result that " processing site " selectivity under some condition such as pH, ionic strength or secondary modification exposes, thereby makes the incident of other nonspecific protease catalysis high degree of specificity.The vacuole proenzyme has been provided the example of a back type by limited proteoclastic activation.

Form takes place or differentiation may be defined as modulated a series of incidents, and this incident causes organism to change to another state from a state.Although can not establish the direct relation between proteolytic enzyme and the form result in many cases, present evidence is thought the participation of significant proteolytic enzyme in the fungi form takes place; In observing differentiation protein renewal widely, sporulation and the spore germination, think that proteolytic enzyme participates in normal processes directly, form (Deshpande, 1992) as most advanced and sophisticated branch of mycelia and partition.

Aspergillus species particularly Aspergillus fumigatus (A.fumigatus) and flavus (A.flavus) may be the pathogenic agent (Bodey and Vartivarian, 1989) that is called the numerous disease of aspergillosis in the humans and animals.Think that repeatedly proteolytic enzyme is relevant with the toxicity of Aspergillus fumigatus and flavus, it interrelates as the toxicity that has many researchs with excretory proteolytic enzyme and bacterium.In fact, the people of most of aspergillus infections is characterised in that its pulmonary parenchyma that mainly is made of collagen protein and elastin is by extensively degraded people such as (, 1994) Campbell.Research has concentrated on the supposition of secreted proteolytic enzyme in Aspergillus fumigatus and flavus toxicity and has used, this Aspergillus fumigatus and flavus are main human pathogens, and knownly have elastin lytic activity (elastinolytic) and a Collagenase (collagenic) active people such as (, 1993) Kolattukudy.These elastin cracking (elastinolytic) are active in external demonstration relevant with infectivity to mouse people such as (, 1984) Kothary.Known two kinds of secreted protein enzymes are by Aspergillus fumigatus and immobilizing Aspergillus flavus for Producing, i.e. alkaline serine protease (ALP) and neutral metal proteolytic enzyme (MEP).In Aspergillus fumigatus, with two genes of these proteolytic enzyme of coding separate, characterization and destruction (people such as Reicherd, 1990; People such as Tang, 1992,1993; People such as Jaton-Ogay, 1994).Yet when comparing with the wild-type strain, alp mep double-mutant does not show difference on pathogenic.Therefore, can draw sure conclusion think the secretor type Aspergillus fumigatus proteolytic enzyme of external evaluation for tissue invade be not must the factor people such as (, 1994) Jaton Ogay.Although Aspergillus fumigatus only accounts for the sub-fraction of airborne mould spores, it is the most frequent isolating fungi from lung and partition (people such as Schmitt, 1991).Other explanations of fungitoxicity be can be the parasitic growth that condition (temperature and nutrient) in the segmental bronchus helps Aspergillus fumigatus.As a result, when host's pathogenic defence was slackened by immunosuppression processing and disease such as AIDS, the invasive aspergillosis then can be circumstantial incident.

Known have 4 main proteolytic enzyme kinds, and this kind is named with the major function group of its avtive spot: ' Serine ', ' sulfydryl ' or ' halfcystine ', ' aspartic acid ' or ' carboxyl ' and ' metal ' proteolytic enzyme.The 244th and 248 parts (A.J.Barrett ed, 1994 and 1995) to the detailed visible Enzymology method of current summary (Methodsin Enzymology) of the main kind of these proteolytic enzyme, less important kind and non-classified proteolytic enzyme.

The specificity of proteolytic enzyme

Except that the catalyst mechanism of proteolytic enzyme, other importances of proteolytic ferment are specificitys of proteolytic enzyme.The specificity of proteolytic enzyme shows that this proteolytic enzyme may any substrate of hydrolysis.20 natural amino acids provide a lot of possibilities that constitute peptide.For example can constitute 400 dipeptides and 800 different tripeptides etc. with 20 amino acid people.About longer peptide, possible number will become almost unlimited.Some proteolytic enzyme is only in the unusual special sequence of certain location hydrolysis.The interaction of proteolytic enzyme and peptide substrates can comprise 1 to 10 amino-acid residue of peptide substrates.Have even the residue of more and the interactional substrate of proteolytic enzyme about big protein substrate.Yet, this may be included in avtive spot in conjunction with outside crack (cleft) with the low specific interaction of proteolytic enzyme residue.Usually, specific recognition is defined in linear peptide, and this peptide is in the combination of protease activities site.

Describe substrate and the interactional nomenclature of proteolytic enzyme by Schechter and Berger in proposition in 1967 (Biochem.Biophys.Res.Com., 1967,27,157-162), and be widely used in the document now.In this system, think that the amino-acid residue of peptide substrate combines with so-called sublocus (sub-sites) at avtive spot.As usual, the sublocus on these proteolytic enzyme is called as S (representative sublocus), and corresponding amino-acid residue is called as P (representative peptide).The amino-acid residue of the N-end side of the key that easily splits is numbered P3, P2, P1, and the amino-acid residue of those C-end sides is numbered P3 ', P2 ', P1 '.P1 and P1 ' are the amino-acid residues that is positioned near the key place of easily splitting.Substrate residue around the cleavage site can be numbered up to P8 then.Corresponding sublocus be will combine on the residue complementary proteolytic enzyme with substrate and S3, S2, S1, S1 ', S2 ', S3 ' etc. will be numbered.The preferred property of sublocus has been determined the preferred property of cutting the proteolytic enzyme of some specific aminoacid sequence in special site in the binding site peptide point.The aminoacid sequence of substrate should be consistent with the preferred property of being showed by sublocus.Specificity to some substrate obviously depends on the binding affinity of substrate and the key hydrolysis rate that easily splits subsequently.Therefore, proteolytic enzyme shows that by its kcat/Km ratio this ratio is known as the specificity constant better usually to the specificity of some substrate.In this specificity constant, kcat represents turnover ratio, and Km is a dissociation constant.

Except that participating in catalysis and bonded amino-acid residue, proteolytic enzyme contains many other necessary amino-acid residues.Some residues are crucial in folding, and some residues are kept the total three-dimensional structure of proteolytic enzyme, and some residues may relate to the adjusting of proteolytic activity, and some residues can make proteolytic enzyme be positioned special position.Many proteolytic enzyme contain the binding site of one or more metal ions outside avtive spot.These metal ions often work the constitutionally stable effect that makes.In addition, extensively glycosylation of excretory eukaryotic microorganisms proteolytic enzyme.The glycosylation that N-is connected with O-all has generation.But the glycosylation auxiliary protein folding, can increase solubility, prevent to assemble and stablize mature protein.In addition, glycosylated degree can influence secretion and proteinic water combination.

The adjusting of proteolytic activity

The proteolytic activity of the proteolytic enzyme of a great deal of is subjected to extensively regulating to avoid disadvantageous proteolysis infringement.On some degree, this adjusting betides transcriptional level.For example in fungi, secreted protein enzyme gene transcription is for the carbon and the nitrogenous source sensitivity of outside, and the gene of Codocyte endoproteinase is insensitive.External pH is felt by fungi, and some genes are by pH regulator.In this process, transcriptional protein plays a key role.The proteinic proteolysis process of this instrumentality often is the switch that makes instrumentality protein open or close.

Proteolytic enzyme is carried out intramolecularly and intermolecular adjusting.This means that some amino acid in the proteolytic ferment molecule is necessary for this adjusting.Proteolytic enzyme is generally synthetic with the bigger precursor that is known as proenzyme, non-activity in this precursor catalysis.Usually make the peptide chain of precursor protein kinase inactive extend at the N-terminal of proteolytic enzyme.This precursor is known as preceding protein (pro-protein) better.Many protein by this approach processing will go out from emiocytosis, so they contain a signal sequence (presequence) in addition, thereby the former former form of protein of precursor is synthetic completely.Except that making the proteolytic enzyme non-activity, propetide often is necessary for the mediation effective folding.Examples of proteases comprises serine protease (α crack protein enzyme, subtilisin, aqualysin, prohormone convertase), thiol proteinase (cathepsin L and Crewe proteolytic enzyme), aspartate protease (protease A and cathepsin D) and metalloprotease.In addition, propetide can work in cell traffic individually or with signal peptide.It can promote can promote to stride the transhipment of film with the interaction of cell chaperone or it.The size of the extension that the preceding protein of precursor is former changes the variation that certain degree is arranged in the scope of small peptide and polypeptide, polypeptide wherein can be used as autonomous folding unit and exists.Especially, after the proteolytic enzyme cutting, remain strong proteinase inhibitor even often observe these bigger extensions.Even observe after cutting, but also accessory protein enzyme correct folding of this propetide.Can think the proteic function of this propetide performance molecular chaperone, and the separation of this propetide or extra coexpression may be favourable for the production of proteolytic enzyme.

Proteolytic enzyme in being secreted into substratum and remain on tangible difference on the adjusting level between the intracellular proteolytic enzyme.The proteolytic enzyme that is secreted in the substratum is no longer controlled after activation usually, and is simple relatively on its molecular structure of being made up of a spherical module usually therefore.The pair cell endoproteinase must carry out successive control to avoid the infringement of pair cell.Opposite with the proenzyme of secreted protein enzyme, in complicated adjusting proteolytic enzyme more, very large polypeptide section can be inserted between the signal and proenzyme activation domain of proteolysis module.Structure function studies show that this non-proteolytic enzyme part can interact with macrostructure, film, cofactor, substrate, effector, inhibitor, ionic in participation, and these interact and regulate the active of proteolysis modules or its (they) proenzyme and activate.The non-proteolytic module is showed noticeable change on size and structure.Number of modules can not rely on the proteolysis module and exists.Therefore can think that this module is corresponding to folding autonomous independent structures and functional unit.The value of this module tissue is that the acquisition of new module can give receptor protein new binding specificity, and can cause the noticeable change in its activity, adjusting and the target.Can reorganize by module in order to form new interaction, adjusting, specificity and/or target, the applied molecular biology instrument makes full use of the principle of module tissue protein lytic enzyme.Although this extra module is that N or C-terminal extend usually, also observed big section insertion in the external rings of catalyst structure domain.It is believed that equally in this case the main folding still representative of proteolytic enzyme forms the basic topological framework of functional protein hydrolysis entity, and this insertion can be regarded as the substructure that is folded in proteolysis module surface.

Molecular structure

Organize than the module of larger protein in principle and have universal law in essence.Special in bigger multimode framework, general proteolysis module shows that mean size is 100～400 amino acid.This be secreted into substratum in most of globular preteins lytic enzymes big or small consistent.As discussing in the above, the polypeptide module is a polypeptide fragment, this fragment collapsible and the performance independent community function.Another term of this module is a structural domain.Yet structural domain uses in the situation wider than module.Be often referred to the part of polypeptide chain as used herein nomenclature structure territory, this polypeptide chain has been described general folding topological framework in three-dimensional structure.In a protein, structural domain interacts on multiple degree, but more not extensive than the interaction of the structural element in the structural domain.Other term such as subdomain and folding unit also are used for document.Similarly, can be observed and manyly have same functional especially protein and can have identical structural domain.This structural domain can be discerned from the primary structure that shows some sequence pattern, and this sequence pattern is typical for special structural domain.Typical example be mononucleotide in conjunction with folding, cellulose binding domain, helix turn helix DNA in conjunction with primitive, zinc refer to, EF hand, film anchor.Module refers to that those expections can fold and the structural domain of autonomous performance function.Skilled in the art will recognize that how from other biological or species, this structure and the common available computer software of homologous sequence to be identified special structural domain in primary structure by using.

Although multimode or Multidomain protein can be rendered as a string pearl, observed the assembling of more complicated in fact structure.Under multiple pearl was positioned at situation on the identical polypeptide chain, these pearls were commonly called module or structural domain.When pearl be not positioned at one with identical polypeptide chain on, but when forming set, so the term subunit is used in reference to this pearl by noncovalent interaction.Subunit can be by one or identical genetic transcription, perhaps by different genetic transcriptions.This multimode protein can be changed into proteolysis processing after causing the transcribing of a plurality of subunits.Independent subunit can be made up of a plurality of structural domains.Usually, 100-300 amino acid whose less globular proteins only is made up of a structural domain usually.

The molecular classification of proteolytic ferment

Usually proteolytic enzyme is classified according to its molecular property or according to its functional property.Molecular classification is based on the primary structure of proteolytic enzyme.Proteinic primary structure is represented its aminoacid sequence, and this sequence can be derived from the nucleotide sequence of corresponding gene.Extensively explore similarity in the primary structure and can notice similarity in catalyst mechanism and other character, this in addition may extend into functional property.Term family is used to describe a histone enzyme, these protease-based between its primary structure similarity and show evolutionary relationship.The member who it is believed that such family is by occurring by the evolution of divergence from identical ancestors.In a family, the primary structure meticulousr based on sequence more further marked off subfamily.Classification proteolytic enzyme according to three dimensional fold can comprise secondary structure, tertiary structure and quaternary structure.Usually, the classification on the secondary structure is confined to the content and the general direction of secondary structure element.The similarity of tertiary structure has caused the identification to superfamily or special group.One group of family of common ancestor is thought to have by superfamily or special group, and this is that dimension is folding because they all show common 3-.Usually, tertiary structure is more conservative than primary structure.As a result, the similarity of primary structure does not always reflect similar functional property.In fact, thus functional property is can be basically divergent to cause interesting new property.Quaternary structure is not used for the multiple protein enzyme is classified as yet at present.This may be owing to the preference of structural database for simple globular proteins.Activate, the many proteolysis system of adjusting or complex reaction cascade may be made up of a plurality of structural domains or subunit.Universal law in the structure organization of this protease system can cause novel classification.

According to specific classification

When not having sequence information, proteolytic enzyme has been carried out polytype functional classification.With reference to the classification carried out of catalytic reaction pair enzyme and name be rule in the enzyme nomenclature.This method also is to (enzyme nomenclature (EnzymeNomenclature), 1992Academic Press, cardinal principle Orlando) in the EC numbering of enzyme.In enzyme nomenclature (Enzyme Nomenclature) 1992, can discern two proteinoid enzymes (EC3.4), promptly those exopeptidases (EC 3.4.11-19) and those endopeptidases (EC 3.4.21-24,3.4.99).Endopeptidase is at the peptide chain interior region cutting peptide bonds away from end.Exopeptidase only cuts residue from the end of peptide chain.Exopeptidase in the N-end effect can discharge an amino-acid residue, a dipeptides or a tripeptides, and is called as aminopeptidase (EC3.4.11), dipeptidyl peptidase (EC 3.4.14) and three peptidyl peptases (EC 3.3.14) separately.Begin amino acid whose proteolytic enzyme of peptide processing release from C-terminal and be called as carboxypeptidase (EC3.4.16-18).Peptidyl dipeptidase (EC 3.4.15) is removed a dipeptides from C-terminal.Outward-and interior-peptase become one then and to be pepx (EC 3.4.13), it only specifically cuts dipeptides in its amino acid half point.ω peptase (EC 3.4.19) remove to substitute, cyclisation or the terminal residue that connected by isopeptide bond.

Except the position of proteolytic enzyme cutting peptide chain, for the proteolytic enzyme of each type, can be based on the further differentiation of the characteristic of preferred amino acids residue in the substrate.Usually people can distinguish have widely, medium and narrow specific proteolytic enzyme.Some proteolytic enzyme are simply with the specified protein of their hydrolysis or polypeptide and name, as M-Zyme, collagenase, elastoser.Narrow specificity can be constrained to a special amino acid or special sequence of removing or cut respectively.When proteolytic enzyme showed special preferred property to amino acid of P1 or P1 ' position, this amino acid whose name can be qualification person (qualifier).For example, the prolyl aminopeptidase is removed proline(Pro) (proline(Pro) is the P1 residue) from the N-terminal of peptide.When the key of the imino-side of having cut proline(Pro), used X-Pro or proline(Pro) (proline(Pro) is a P1 ' residue), removed proline(Pro) from C-terminal as proline carboxypeptidase.Prolyl endopeptidase (or Pro-X) cuts in the proline(Pro) back, and proline(Pro) endopeptidase (X-Pro) cuts in the proline(Pro) front.The amino-acid residue of the peptide bond front of easily splitting instigates carboxylic group to the contributive amino-acid residue of peptide bond.The amino-acid residue of the peptide bond back of easily splitting instigates amino group to the contributive amino-acid residue of peptide bond.According to common convention, amino acid chain is from N-terminal (initial) to C-terminal (termination) thereby and is numbered.Endo-protease also shows significantly preferred property for the special amino acid of P1 and P1 ' position, as glycyl endopeptidase, peptide acyl Methionin endopeptidase, glutamy endopeptidase.This exoproteinase can show preferred property to certain group amino acid of sharing some similar part.Such one group of preferred amino acids can comprise hydrophobic amino acid, bulky hydrophobic amino acid, little hydrophobic or only for the amino acid of little amino acid, big positive charge etc. only.Except that preferred property, also there is special preferred or eliminating by the preferred residue of other sublocus on the proteolytic enzyme to P1 and P1 ' residue.These multiple preferably can causing only simultaneously can be satisfied the very specific proteolytic enzyme of multiple sequence in conjunction with needs to those.Usually should be realized that proteolytic enzyme is suitable miscellaneous enzyme.Even very specific proteolytic enzyme also may cut the peptide of the preferred property of this proteolytic enzyme of not deferring to common observation.In addition, should be realized that envrionment conditions such as pH, temperature, ionic strength, water activity, these all can influence the preferred property of proteolytic enzyme whether solvent, competitive substrate or inhibitor.Envrionment conditions not only influences proteolytic enzyme, and influences the approach that protein substrate is presented to proteolytic enzyme.

Classify with catalyst mechanism.

Proteolytic enzyme can segment on the basis of its catalyst mechanism.Need know for above-mentioned classification based on each catalyst mechanism, specificity all causes the further segmentation to each class mechanism.Known have 4 main proteolytic enzyme kinds, and name: serine protease (EC 3.4.21 endopeptidase by the major function group of its avtive spot, EC 3.4.16 carboxypeptidase), sulfydryl or L-Cysteine HCL Anhydrous (EC 3.4.22 endopeptidase, EC 3.4.18 carboxypeptidase), carboxyl or aspartate protease (EC 3.4.23 endopeptidase) and metalloprotease (EC 3.4.24 endopeptidase, EC 3.4.18 carboxypeptidase).Member to each catalytic type of proteolytic enzyme has distinctive inhibitor.These little inhibitor are the amino-acid residue of modified protein enzyme active sites irreversibly.For example, serine protease by with the Phenylmethylsulfonyl chloride (PMSF) and diisopropylfluorophosphate (DFP) inactivation of active ser reaction, yet the Histidine of chloromethyl ketone (chloromethylketone) derivative and catalysis triplet reacts.Phosphoramidon and 1,10 phenanthroline generally suppress metalloprotease.Suppress to be shown as usually aspartate protease by stomach en-.E64 suppresses thiol proteinase specifically.Amastatin and bestatin suppress multiple aminopeptidase.Even in a catalysis kind, also observed the suitable variation of proteolytic enzyme to the susceptibility of inhibitor.To a certain extent, this can be relevant with the specificity of proteolytic enzyme.Under the binding site structure prevented the situation of inhibitor near catalytic site based on mechanism, this proteolytic enzyme was escaped from suppress, and has stoped the evaluation based on inhibiting mechanism type.For example chymotrypsin inhibitor is the strong inhibitor with the active serine protease of chymotrypsin-like, elastase inhibitor suppress elastoser such as serine protease and not with trypsinase or chymotrypsin protein enzyme reaction, 4 acid amides PMSF (APMSF) only suppress to have the specific serine protease of trypsin-like.Broad description to inhibitor purposes in the proteolytic enzyme classification comprises Barret and Salvesen, proteinase inhibitor (Proteinase Inhibitors), Elsevier Amstardam, 1986; Bond and Beynon (eds), proteolytic ferment, a kind of practical methods (Proteolytic Enzymes, A Practical Approach), IRL Press, Oxford, 1989; Enzymology method (Methods in Enzymology), eds E.J.Barret rolled up in 1994 244 and nineteen ninety-fives 248 roll up; E.Shaw, cysteinyl proteolytic enzyme and selectivity inactivation thereof (Cysteinyl proteinase and their selectiveinactivation), Adv Enzymol.63:271-347 (1990).

According to the suitableeest action condition classification

The needs of the catalyst mechanism of proteolytic enzyme and its conformation integrity have determined mainly this proteolytic enzyme can use under which kind of condition.The proteolytic enzyme of finding the suitableeest effect under application conditions is a main challenge.Usually the proteolytic enzyme condition that must act on is not the suitableeest, and has represented the compromise between the optimum condition of the ideal conditions of concrete application and proteolytic enzyme really.Except that the special character of proteolytic enzyme, will be appreciated that the condition that also depends on of presenting of protein substrate, and determined that equally also which kind of condition is the most effective for proteolysis.Comprise as pH dependency, temperature dependency, to the sensitivity of metal ion or dependency, ionic strength, salt concn, solvent compatibility with the specification of using relevant enzyme.Another factor of main importance is the activity specific of proteolytic enzyme.The activity specific of proteolytic enzyme is high more, and the required enzyme of specific transformation is few more.Lower enzyme require means lower cost and lower protein contamination level.

PH is a main parameter of proteolytic enzyme performance in determining to use.Therefore, the pH dependency is the important parameter of classification proteolytic enzyme.The main monoid of recognizing is aspartic protease, neutral protease, Sumizyme MP and high alkaline proteases.The suitableeest pH only mates to a certain degree with proteolysis mechanism, as aspartate protease when being everlasting acidity, show just when, metalloprotease and thiol proteinase are brought into play during to slight alkalinity through the neutral pH of being everlasting the suitablelyyest, and serine protease is active in alkalescence and high alkalinity zone mainly.For each kind is all known exception arranged.In addition, total water activity of system works.The pH of proteolytic enzyme in this scope, to great majority its substrate shows the suitableeest hydrolysis rate in the special environment of proteolytic enzyme under special condition just when being defined as a pH scope.This scope can be narrow, as a pH unit, and a quite wide 3-4 pH unit.Usually pH is just when the characteristic that also depends on protein substrate.Both can be used as turnover ratio and specificity the function of pH and change.For the effect of determining, can away from its just when the time use proteolytic enzyme because the production of undesired peptide has been avoided.Undesired peptide can be as very short peptide or causes the peptide of bitter taste.In addition, narrower specificity can be the reason of selection condition, and this condition departs from the suitableeest condition on about transformation efficiency.Depend on pH, specificity can be narrow, as the special position cutting of before or after special amino acid peptide chain only, or broad, as cutting before or after amino acid whose at a plurality of positions cutting peptide chain or how dissimilar.In fact, the pH dependency can be the important tool of regulating proteolytic activity in application.Under the situation that pH changes during the course, proteolysis can spontaneously stop, and need not further to handle so that the proteolytic enzyme inactivation.In some cases, proteolysis itself can be the driving thing that pH changes.

To the application of proteolytic enzyme very crucial be the stability of its processing and operation.Because proteolytic enzyme stability is influenced by working temperature strongly, so also stability is called thermostability usually.Usually the stability of proteolytic enzyme is presented at how long endoproteinase keeps its proteolytic activity under special condition.Special condition can comprise fermentation condition, at the separation of enzyme and condition, holding conditions, preparation and operation or the application conditions in the processing of downstream.When special condition comprised the temperature of rising, stability was often referred to thermostability.Except that the general reason such as the chemically modified of enzyme deactivation, separate folding, the gathering etc., the subject matter of proteolytic enzyme is that they are easy to carry out from degraded.Especially for the utilization of proteolytic enzyme, temperature is just when the related standards that is the proteolytic enzyme classification.Although different definition is arranged, the most useful commercial definition is in some applications efficient temperature of proteolytic enzyme or temperature range.The proteolytic enzyme productivity is the function of stability and turnover ratio.Temperature in this rising will increase turnover ratio usually, and inactivation will be offset the increase of turnover ratio fast, and finally cause low productivity.The conformational stability of proteolytic enzyme will be determined its service temperature maximum value in the condition of given process.Temperature when proteolytic enzyme loosens its activity conformation is shown as separates folding or fusing point, can determine according to several different methods, as NMR, circular dichroism, spectroscopy, differential scan calorimeter etc.For proteolytic enzyme, separate the folding very big increase of following usually from degradation rate.

In the cryogenic application of needs, proteolytic enzyme can be selected in low high intrinsic activity during to moderate temperature.Because inactivation is low relatively under this condition, so activity can be determined its productivity to a great extent under this condition.Only needing in the process of protease activity in short period, the stability of proteolytic enzyme can be used as the switch that this proteolytic enzyme is closed.In this case, can preferably more unsettled rather than very heat-staple proteolytic enzyme.

Other environmental parameters that can work in selecting suitable proteolytic enzyme can be its susceptibility to salt.With the consistency of metal ion be crucial for some application, this metal ion often is found in the multiple natural materials with low concentration.With regard to metalloprotease, the alternative catalytic metal ion of some ion also reduces or even completely destroy activity especially.In some applications, in order to prevent and the washout of proteolytic enzyme coordinate metal ion, must intentionally add metal ion.Well-known is for enzyme stability and life-span, and dissociating of the calcium of calcium ion to prevent protein bound must be provided.

Most microorganisms show some tolerance that the condition that conforms changes.Result's this biology proteolysis that can produce at least composes and may show similar tolerance.A kind of like this proteolysis spectrum can be covered or only be had by minority the proteolytic enzyme covering of extensive hydrolysis spectrum together by many proteolytic ferments.Consider whole proteolysis spectrums of microorganism, it is highly important that and consider the position.

Location and characterization in the cell of proteolysis process and degraded

From industrial point, have the specific advantage of scale operation power and stress tolerance from the proteolytic enzyme of emiocytosis, this is because they must be survived under the cytoprotective not being subjected to.The leukoprotease of big group can further be subdivided into solubility with membrane-bound.The membrane-bound proteolytic enzyme that is positioned at the film inboard and the outside that comprises.The soluble proteins enzyme can further segment according to the specific cells compartment that they took place in the cell.Because cell shields proteolytic enzyme and environment on some degree, and control intracellular condition because of cell, so intracellular protein is more responsive for big environmental change, and it is just when can be more relevant with condition in the specific cell.The condition of knowing the existing cellular compartment of proteolytic enzyme can show its preferred property.When extracellular protease is no longer needing any adjusting, intracellular protease then often to be subjected to complicated control and adjusting after emiocytosis usually.

About the function of special proteolytic enzyme, its location often is very important; For example, many vacuoles and periplasm protein enzyme participate in protein degradation, and many embrane-associated protein enzymes are important (Suarez Rendeules and Wolf, 1988) in protein processing.

Detailed summary to proteolytic enzyme biological property and evolution has been published in van denHombergh:Thesis Landbouwuniversiteit Wageningen: for improving analysis (An analysis of theproteolytic system in Aspergillus in order to improveprotein production) the ISBN 90-5485-545-2 of protein production to the proteolysis system, herein its integral body is quoted as a reference in Aspergillus.

The proteolytic enzyme problem

To the interested major reason of microbial protease is the relevant expression problem of proteolytic enzyme, and this problem is found in several expressive hosts that are used for biological processing industry.Heterologous host takes this problem on the focus to for the utilization that protein production increases recently by recombinant DNA technology, and this is because it seems that heterologous protein is easilier to be fallen (people such as Archer, 1992 by proteolysis; People such as van den Hormbergh, 1996b).

In yeast saccharomyces cerevisiae, had realized that the participation of proteolytic enzyme problem and several proteolytic enzyme in early days in [20th century] eighties, thereby made the directed gene destruction methods complicated to address this problem.In secretion, protein is exposed in several proteolytic activities that are present in the Secretory Pathway.In addition, in prototroph such as Aspergillus, excretory protein can be exposed in several extracellular protein hydrolytic activities.

The problem of the protein degradation of heterogenous expression has been carried out good proof (van den Hombergh:Thesis LandbouwuniversiteitWageningen: be analysis (An analysis of the proteolytic system in Aspergillus inorder to improve protein product ion) the ISBN 90-5485-545-2 of improvement protein production to the proteolysis system in Aspergillus) in Aspergillus, and has reported the cows milk curds proenzyme, human interferon-alpha-2tPA, GMCSF, IL6, lactoferrin, hen's egg-white lysozyme (lysosyme), pig p1A2, aspergillus niger pectic enzyme (lysase) B, this class problem in the expression of intestinal bacteria (E.coli) enterotoxin B and β-glucoronidase and carrot soft rot Erwinia (Erwinia carotovora) pectate lytic enzyme (pectate lysase) 3.

Proteoclastic problem can solve at the several stages of protein production.Biological processing slip-stick artist can by carry out downstream processing at low temperature, by early stage separated product and proteolytic enzyme or by using proteinase inhibitor to solve the proteolysis problem.These can all cause successfully reducing this problem.Yet problem is not eliminated certainly, and this is because many hydrolysis have taken place in the protein production process in vivo.

Understand proteolysis and how to control in cell, main problem relates to the proteoclastic recognition mechanism of triggering.(allogenic) protein with the proteolysis susceptible on which kind of degree is identified as because of malfolding unusually, if perhaps correct folding not settling because they do not have the place given birth to the special very crucial character of stability is identified as " external source ".The factor of known increase proteolysis speed comprises the adverse circumstance pressure (being the expression of rising, seepage water pressure, toxicant and some heterologous protein of temperature) of nutrient deficiency and multiple other types.In order to handle the relevant expression problem of proteolysis in vivo, as the SOME METHODS that will be described below has proved success.Yet we must keep real " cell of non-proteolytic " firmly in mind and not exist, and this is because participate in many basic metabolism and " house keeper (housekeeping) " reaction by the proteolysis of intracellular protease.Therefore in reducing proteoclastic process, must analyze the genetic background potential secondary action that causes proteolysis to reduce, this secondary action can cause protein production to reduce (reducing or sporulation as growth velocity).

In the filamentous fungus expressive host, destroy proteolytic enzyme

Berka and colleague thereof (1990) have described the clone and the destruction of Aspergillus awamori (A.awamori) pepA gene.Recently, 3 destructive aspartyl proteases in the aspergillus niger have been described.Disrupting agent (disruptant) to main extracellular aspartyl protease and main vacuole aspartyl protease is described.Generated the triple disrupting agents of double sum by reorganization, and checked the proteinic albumen zymogram of aspergillus niger pectic enzyme PELB and expression and secretion, this PELB protein is for the unusual susceptible (people such as van denHombergh, 1995) of proteolytic degradation.The destruction of pepA and pepB causes the activity of extracellular protease to reduce 80% and 6% respectively.In Δ pepE disrupting agent, other (vacuole) protease activity is also owing to the proteolysis cascade to other vacuole protein enzymes is seriously influenced equally.The active PELB external degradation in the extracellular of reducing with minimizing relevant with the improved expression in vivo of pelB (people such as van den Hombergh, 1996f).

Protease-deficient (prt) mutant filamentous fungus

Studied whether protein production is improved in several Aspergillus protease-deficient mutant.Archer and colleague thereof have described the proteolysis that the hen egg white N,O-Diacetylmuramidase reduces in the aspergillus niger prt double-mutant supernatant liquor that is generated by Mattern and colleague (1992) thereof, although and infer that degraded still exists, this degraded has reduced significantly.People (1995) such as Van denHombergh show that the external degradation of aspergillus niger PELB has all reduced in their isolating all 7 prt complementation groups.In fact in prtB, prtF and prtG mutant, do not observe degraded.Recently, in the complementation group (prtA-F) of 6 checks, all show the improvement of pelB genetic expression, and in prtB, prtF and prtG mutant, observed maximum expression level.Except that single mutant, it contains the residual extracellular protein hydrolytic activity of comparing with wild-type activity at 2-80%, by reorganization with take turns mutagenesis by another and generate double-mutant.Through this method, several prt double-mutants have been carried out selecting and further characterization, this shows the further minimizing of comparing the PELB degraded with its parental generation strain.

Without destroying or mutagenesis elimination protease activity, reduce proteolysis and also can realize by relevant proteolytic activity decrement is regulated.This can realize by other adjusting sequences that change promotor or gene hereditarily.As by shown in Fraissinet-Tachet and the colleague (1996) thereof, the extracellular protease of aspergillus niger is all suppressed by the carbon catabolite and the nitrogen metabolism thing suppresses adjusting.Nutritive deficiency also causes the fierce increase of proteolysis speed total in the cell, and this also is applicable to the shortage nutrient but has proteinic cell that it does not need or only need a small amount of nutrition under starvation conditions.Can report proteoclastic minimizing in the expression strategy of high expression level on the substratum that is containing high glucose and ammonium concentration making.Several composing type glycolysis-promotors (gpd and pkiA) are highly expressed under these conditions, and also can be used for driving (allogenic) genetic expression in continuously fermenting.The different proteolytic enzyme of degree influence that the nutrient deficiency type of being forced can change this means that the importance of nutrient condition in given process depends on the proteoclastic type that relates to.Therefore specific proteolysis can be induced by the condition of substrate restriction, and this substrate restriction is through being usually used in many large scale fermentation processes.

The proteolytic enzyme problem can partly be solved by a kind of above-mentioned or multiple strategy now.Yet the residual proteolytic activity of the proteolytic ferment of Jian Dinging has not still constituted the subject matter in this area yet.In order further to reduce disadvantageous proteoclastic level, be starved of in this area new proteolytic enzyme is identified, this proteolytic enzyme is responsible for the proteinic degraded of homology and heterogenous expression.The invention provides the new proteinase gene sequence of this proteins encoded enzyme.In case the primary sequence of known new proteinase gene then can adopt one or more above-mentioned recombinant DNA strategies to produce the mutant that (gene knockout) reduces proteolytic activity.

Although proteolytic enzyme is widely used in most of commercial runs, existing enzyme also has remarkable shortcoming at least a following character.

In the time of in joining animal-feed, existing proteolytic enzyme is not enough to opposing and is present in as the digestive ferment in pig and poultry stomach and intestine (GI) road.

On the other hand, existing available enzyme extrude or granulating in be not enough to resist specific (height) temperature and (height) pressure condition of employing.

Equally, existing enzyme does not have enough activity when pH scope 3-7, and this condition is general in numerous food product, drink product and the GI road most of animals.

According to the another one aspect, the specificity of existing available proteolytic enzyme is very limited, and this causes existing enzyme can not degrade or dissolve some " protease resistant " protein, thereby causes peptide or amino acid to yield poorly.In addition, having new specific proteolytic enzyme makes new peptide to synthesize.

The another one shortcoming of existing available enzyme is its low activity specific.

Therefore obviously existence is to a large amount of needs of proteolytic enzyme for a large amount of application, and this proteolytic enzyme has more resistance to digestive ferment, high temperature and/or pressure, and performance is to the new specificity in its hydrolysis site.The invention provides this kind of enzyme.

Goal of the invention

The object of the invention provides the new polynucleotide of the new proteolytic enzyme of coding.Further purpose provides the proteolytic enzyme of natural and recombinant production and produces the recombinant bacterial strain of these enzymes.This bacterial strain also can be used for quickly or produces traditional tunning with higher output.Another object of the present invention provides defective filamentous fungal strains in producing according to proteolytic enzyme of the present invention.This bacterial strain can be used for more effectively production allos or homologous protein.Same antibody and fusion polypeptide and preparation and use are parts of the present invention according to the method for polynucleotide of the present invention and polypeptide.

Summary of the invention

The invention provides the new polynucleotide of the new proteolytic enzyme of coding.

More specifically, the invention provides polynucleotide, these polynucleotide have (preferably under height stringency condition) with according to being selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or being selected from the nucleotide sequence of sequence hybridization of the sequence of SEQ ID NO:58～SEQ ID NO:114.Therefore, the invention provides nucleic acid, this nucleic acid with have according to the sequence that is selected from the sequence of SEQ IDNO:1～SEQ ID NO:57 or is selected from the sequence of SEQ ID NO:58～SEQ IDNO:114 about 60%, be preferably 65%, more preferably 70%, be more preferably 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology.

In an embodiment that is more preferably, the invention provides isolating polynucleotide, these polynucleotide can be preferably in the Aspergillus and obtain preferred especially aspergillus niger from filamentous fungus.

In one embodiment, the invention provides isolating polynucleotide, these polynucleotide comprise the nucleic acid encoding sequence, and this polypeptide has the aminoacid sequence that is selected from SEQ ID NO:115～SEQ IDNO:171 or its function equivalent.

In a further preferred embodiment, the invention provides isolating polynucleotide, this polynucleotide encoding is according at least one functional domain that is selected from the polypeptide of sequence of SEQ ID NO:115～SEQ ID NO:171 or its function equivalent.

In a preferred embodiment, the invention provides according to the proteinase gene that is selected from the sequence of SEQ IDNO:1～SEQ ID NO:57.In yet another aspect, the invention provides polynucleotide, be preferably aspergillus niger proteolytic enzyme or this variant polypeptides or cDNA fragment that coding is selected from SEQ ID NO:115～SEQ ID NO:171.In a preferred embodiment, this cDNA has the sequence that is selected from SEQ ID NO:58～SEQ ID NO:114 or its function equivalent.

Coding also can obtain by following method according to the genomic clone of polypeptide of the present invention, promptly select suitable probe, be used for increasing specifically corresponding to according to the genome area of SEQ ID NO:1～SEQ ID NO:57 or its segmental any one sequence, with this probe under proper condition with suitable biology such as Aspergillus such as aspergillus niger in the genomic dna that obtains hybridize, by as PCR (polymerase chain reaction) amplification purpose fragment after segmental purifying and clone to increasing.

In embodiment preferred further, the invention provides polynucleotide, these polynucleotide comprise the encoding sequence according to genome polynucleotide of the present invention, are preferably the polynucleotide sequence that is selected from SEQ ID NO:58～SEQ ID NO:114.

In another preferred embodiment, the invention provides cDNA, this cDNA can clone in suitable host living beings such as aspergillus niger and express and obtain by the sequence that will be selected from SEQ ID NO:1～SEQ ID NO:57.

Also can in suitable host living beings such as aspergillus niger, clone and express according to a polypeptide of the present invention and obtain by the sequence that will be selected from SEQ ID NO:1～SEQ IDNO:57.

The present invention also relates to comprise according to polynucleotide sequence of the present invention and can be used for increasing or survey primer, probe and segmental carrier according to DNA of the present invention.

In a further preferred embodiment, carrier is provided, wherein functionally be connected with the adjusting sequence according to polynucleotide sequence of the present invention, this adjusting sequence is suitable for amino acid sequence coded and expresses in appropriate host cell such as aspergillus niger or aspergillus oryzae (A.oryzea).The present invention also provides the method for preparation according to polynucleotide of the present invention and carrier.

The present invention also relates to the host cell of recombinant production, this cell contains with good grounds allos of the present invention or homologous polynucleotide.

In one embodiment, the invention provides recombinant host cell, wherein the expression according to proteolytic enzyme of the present invention significantly reduces, and perhaps wherein protease activities reduces, perhaps wherein proteolytic enzyme even inactivation.This recombinant chou is particularly useful for the expression of homology or heterologous protein.

In another embodiment, the invention provides recombinant host cell, wherein the expression according to proteolytic enzyme of the present invention significantly increases, and perhaps wherein protease activities increases.This recombinant chou is particularly useful for the expression of homology or heterologous protein, if become rate-limiting step in this essential proteolysis cutting, maturation is then seriously hindered so.

In another embodiment, the invention provides a kind of host cell of recombinant production, this cell contains with good grounds allos of the present invention and homologous dna, be preferably the protein DNA that coding has signal sequence, and wherein this cell can be produced according to functional protein enzyme of the present invention, be preferably the cell that can make, for example comprise copy number purpose Aspergillus bacterial strain according to gene of the present invention or cDNA increase according to proteolytic enzyme overexpression of the present invention.

In another embodiment, the invention provides a kind of host cell of recombinant production, this cell contains with good grounds allos of the present invention and homologous dna, and wherein this cell can be secreted according to functional protein enzyme of the present invention, be preferably can overexpression and secretion according to the cell of proteolytic enzyme of the present invention, for example comprise the copy number purpose Aspergillus bacterial strain that increases according to gene of the present invention or cDNA.

In another aspect of the present invention, provide the polypeptide of purifying.Polypeptide according to the present invention comprises by the polypeptide according to polynucleotide encoding of the present invention.Especially preferred according to the polypeptide of sequence that is selected from SEQ ID NO:115～SEQ ID NO:171 or its function equivalent.

The present invention also provide can with the antibody of polypeptide reaction according to the present invention.These antibody can be polyclonal, but especially preferred monoclonal antibody.This antibody is particularly useful for purifying polypeptide according to the present invention.

The fused protein that comprises the polypeptide according to the present invention also within the scope of the invention.The present invention also provides the method for preparation according to polypeptide of the present invention.

The invention further relates to by detecting and whether exist, perhaps diagnose aspergillosis by detecting whether to exist according to DNA of the present invention or its fragment or function equivalent according to polypeptide of the present invention or its function equivalent.

The present invention also relates to according to the purposes in the described herein commercial run of proteolytic enzyme of the present invention.

Detailed Description Of The Invention

Polynucleotide

The invention provides the polynucleotide of proteins encoded enzyme, this proteolytic enzyme has aminoacid sequence or its function equivalent that sequence is selected from SEQ ID NO:115～SEQ ID NO:171.The sequence of these genes is passed through the order-checking of the genomic clone that obtains from aspergillus niger is determined.The invention provides the polynucleotide sequence of the encoding gene that comprises these proteolytic enzyme of encoding and global cDNA sequence thereof and its encoding sequence.Therefore, the present invention relates to isolating polynucleotide, these polynucleotide comprise nucleotide sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or the sequence that is selected from SEQID NO:58～SEQ ID NO:114 or its function equivalent.

More specifically, the present invention relates to isolating polynucleotide, these polynucleotide can be hybridized with the sequence that is selected from the polynucleotide sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from SEQ ID NO:58～SEQ ID NO:114 under the stringency condition, are preferably under height stringency condition.Advantageously, this polynucleotide can particularly obtain from aspergillus niger from filamentous fungus.More specifically, the present invention relates to isolating polynucleotide, these polynucleotide have the nucleotide sequence that basis is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from the sequence of SEQ ID NO:58～SEQID NO:114.

The present invention also relates to isolating polynucleotide, at least a basis of this polynucleotide encoding is selected from the functional domain of the SEQ ID NO:115～sequence of SEQ ID NO:171 or the polypeptide of its function equivalent.

As used herein, term " gene " and " recombination " refer to can be from the chromosomal DNA isolated nucleic acid molecule, and this molecule comprises the open reading-frame (ORF) of coded protein such as aspergillus niger proteolytic enzyme.Gene can comprise encoding sequence, non-coding sequence, intron and adjusting sequence.In addition, gene refers to as isolated nucleic acid molecule defined here.

The Protocols in Molecular Biology of nucleic acid molecule available standards of the present invention separates with sequence information provided herein, and this nucleic acid molecule is as having the sequence that is selected from SEQ ID NO:1～SEQ IDNO:57 or being selected from the nucleotide sequence of the SEQ ID NO:58～sequence of SEQ ID NO:114 or the nucleic acid molecule of its function equivalent.For example, utilize the nucleotide sequence of the sequence that is selected from SEQID NO:1～SEQ ID NO:57 of all or part or the nucleotide sequence of sequence that is selected from SEQ IDNO:58～SEQ ID NO:114 as hybridization probe, hybridization according to nucleic acid molecule available standards of the present invention separates with clone technology and (as is described in Sambrook, J., Fritsh, E.F. and Maniatis, T., molecular cloning: laboratory manual (Molecular Cloning:A Laboratory Manual), 2 ^Nd, ed., Cold Spring Harbor Laboratory, Cold Spring HarborLaboratory Press, Cold Spring Harbor, NY, 1989).

In addition, comprising the nucleic acid molecule that all or part is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from the sequence of SEQ ID NO:58～SEQ ID NO:114 can separate by polymerase chain reaction (PCR) with the synthetic Oligonucleolide primers, and this primer is based in the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or be selected from the sequence information that contains in the sequence of SEQ ID NO:58～SEQ ID NO:114 and design.

Nucleic acid of the present invention can increase as template and the suitable Oligonucleolide primers pcr amplification technology according to standard with cDNA, mRNA or genomic dna.Kuo Zeng nucleic acid can be cloned in the appropriate carriers and with dna sequence analysis and characterize like this.

In addition, corresponding to or can be prepared by the standard synthetic technology with oligonucleotide according to nucleotide sequence hybridization of the present invention, as utilize automatic dna synthesizer.

In a preferred embodiment, isolated nucleic acid molecule of the present invention comprises the nucleotide sequence shown in the sequence that is selected from SEQID NO:58～SEQ ID NO:114.The sequence of sequence that is selected from SEQID NO:58～SEQ ID NO:114 is corresponding to the coding region of aspergillus niger proteolytic enzyme cDNA.This cDNA comprises the sequence of coding according to the aspergillus niger protease polypeptide of the sequence that is selected from SEQ ID NO:115～SEQ IDNO:171.

In another preferred embodiment, isolated nucleic acid molecule of the present invention comprises and is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from nucleotide sequence or its function equivalent complementary nucleotide sequence shown in the sequence of SEQ ID NO:58～SEQID NO:114.

Enough complementary with another nucleotide sequence complementary nucleic acid molecule and its, thus can hybridize with it, and form stable duplex whereby.

One aspect of the present invention is about isolated nucleic acid molecule, this nucleic acid molecule encoding polypeptide of the present invention or its function equivalent such as bioactive fragment or structural domain, and be enough to be suitable for as the PCR primer so that the fragment of amplified nucleic acid molecule or sudden change as the nucleotide sequence and this nucleic acid molecule of hybridization probe with the nucleic acid molecule of identification code polypeptide of the present invention.

" isolating polynucleotide " or " isolating nucleic acid " are DNA or RNA, this DNA or RNA not with two direct adjacency of encoding sequence, and in the naturally occurring genome of the organism that it was derived from it and this encoding sequence directly in abutting connection with (one at 5 ' end, one at 3 ' end).Thereby in one embodiment, isolating nucleic acid comprises 5 ' non-coding (as the promotor) sequence of some or all and the direct adjacency of encoding sequence.Therefore this term comprises as inserting carrier, the plasmid of self-replicating or the recombinant DNA in virus or prokaryotic organism or the Eukaryotic genomic dna, perhaps as the molecule that is separated (as handled the cDNA or the genomic DNA fragment of generation by PCR or restriction endonuclease) of independence and other sequences.It also comprises a kind of recombinant DNA, this recombinant DNA is the part of the hybrid gene of the extra polypeptide of coding, and this polypeptide does not contain cell material, viral material or culture medium (when being produced by recombinant DNA technology) or precursor or other pharmaceutical chemicals (when chemically synthetic) substantially.In addition, " isolating nucleic acid fragment " be not as a naturally occurring nucleic acid fragment and be not found in the native state.

As used herein, term " polynucleotide " or " nucleic acid molecule " mean and comprise dna molecular (as cDNA or genomic dna) and RNA molecule (as mRNA) and the DNA or the RNA analogue that generate with nucleotide analog.Nucleic acid molecule can be strand or double-stranded, but is preferably double-stranded DNA.Nucleic acid can synthesize with oligonucleotide analogs or derivative (as inosine or thiophosphoric acid Nucleotide).This oligonucleotide can be used for having as preparation the nucleic acid of the nuclease resistance of the base pairing ability of change or increase.

Another embodiment of the invention provides isolated nucleic acid molecule, the coding strand antisense of this nucleic acid molecule and proteolytic enzyme nucleic acid molecule such as proteolytic enzyme nucleic acid molecule.Comprise equally within the scope of the invention be the complementary strand of nucleic acid molecule described herein.

The order-checking mistake

Sequence information mentioned herein should straitly be interpreted as comprising the base that mistake is identified like this.Particular sequence disclosed herein can be advantageously used in particularly separating complete gene the aspergillus niger from filamentous fungus, and this can make it be easy to carry out further sequential analysis conversely and identify the order-checking mistake whereby.

Unless otherwise instructed, all all are to determine with the automated DNA sequenator by determined nucleotide sequence that dna molecular is checked order herein, and all are all predicted by as above determined dna sequence dna is translated by the amino acid sequence of polypeptide of the dna molecule encode of determining herein.Therefore, as known in the art, any dna sequence dna, any nucleotide sequence of being determined by this automated method of determining herein all can contain some mistakes.Generally consistent by the nucleotide sequence that automatic gear is determined at least about 90% with the actual nucleotide sequence of the dna molecular of order-checking, more generally be at least about 95%～at least about 99.9% unanimity.Actual sequence can determine that more accurately this method comprises artificial DNA sequence measurement well known in the art by additive method.As being known in the art equally, compare in the nucleotide sequence of determining one insertion or disappearance with actual sequence and just will cause frameshit in the nucleotide sequence translation, thereby will begin with different fully in this insertion or deletion segment by the amino acid of the dna molecular actual coding that checks order by the aminoacid sequence of the nucleotide sequence coded prediction of determining.

Those skilled in the art can identify this wrong base of identifying and know how to proofread and correct this mistake.

Nucleic acid fragment, probe and primer

A part or a fragment that can only comprise nucleic acid molecule according to nucleic acid molecule of the present invention, this nucleic acid molecule is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from the sequence of SEQID NO:58～SEQ ID NO:114, for example can be used as the segmental fragment of the part of probe or primer or this proteinase gene of encoding.Make it possible to generate be designed for from the nucleotide sequence that proteinase gene and cDNA are determined and identify and/or clone other proteolytic enzyme family member and from the probe and the primer of the proteolytic enzyme homologue of other species.This probe/primer generally comprises the oligonucleotide of basic purifying, this oligonucleotide generally comprise preferred under height stringency condition with a nucleotide sequence at least about 12 or 15, be preferably about 18 or 20, be preferably about 22 or 25, more preferably about 30,35,40,45,50,55,60,65 or 75 or more successive Nucleotide nucleotides sequence column region of hybridizing, nucleotides sequence wherein be listed in the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 be selected from the sequence of SEQ IDNO:58～SEQ ID NO:114 or its function equivalent in show.

The transcript or the genome proteolytic enzyme sequence that can be used for surveying or homologous protein identical based on the probe of proteolytic enzyme nucleotide sequence as coding in other biological.In preferred embodiments, this probe further comprises the labelling groups that adheres to thereon, can be radio isotope, fluorescent chemicals, enzyme or enzyme cofactor as this labelling groups.This probe also can be used as the part of the diagnostic check test kit of the cell of identifying the expressing protein zymoprotein.

Identity and homology

Term " homology " and " identity per-cent " exchange use herein.For purpose of the present invention, in order to determine the identity per-cent of two aminoacid sequences or two nucleotide sequences, in order to carry out optimum ratio than sequence being compared (, in the sequence of first amino acid or nucleotide sequence, having introduced breach) as right in order to carry out with the optimum ratio of second amino acid or nucleotide sequence.Then amino-acid residue on corresponding amino acid position or the nucleotide position or Nucleotide are compared.When the position in first sequence by with second sequence in when identical amino-acid residue or Nucleotide occupy on the corresponding position, this molecule is same on this position so.Identity per-cent between two sequences is the function (that is the overall number of the number/position of % identity=same position (being the lap position) x100) of the total same position number of sequence.Preferably, these two sequences are isometric.

The technician will know that several different computer softwares can be used for the homology between definite two sequences.For example, the definite available mathematical algorithm of sequence comparison and identity per-cent is realized between two sequences.In a preferred embodiment, identity per-cent between two aminoacid sequences is determined with Needleman and Wunsch (J.Mol.Biol. (48): 444-453 (1970)) algorithm, this algorithm has been incorporated in the GAP program of GCG software package (can obtain from http://www.gcg.com), uses Blossom 62 matrixes or PAM250 matrix and 16,14,12,10,8,6 or 4 breach flexible strategy (gap weight) and 1,2,3,4,5 or 6 length flexible strategy (length weight).The technician is appreciated that all these different parameters will produce slightly different result, but total identity per-cent of two sequences and change indistinctively when using different algorithms.

In the another one embodiment, identity per-cent between two nucleotide sequences is determined with the GAP program in the GCG software package (can obtain from http://www.gcg.com), uses NWSgapdna.CMP matrix and 40,50,60,70 or 80 breach flexible strategy and 1,2,3,4,5 or 6 length flexible strategy.In another embodiment, the identity per-cent of two amino acid or nucleotide sequence is with E.Meyers and W.Miller (CABIOS, 4:11-17 (1989)) algorithm is determined, this algorithm has been incorporated into the ALIGN program (2.0 editions) (can obtain from http://vega/igh.cnrs.fr/bin/align-guess.cgi), uses PAM120 flexible strategy (weight) residue table, notch length point penalty 12 and breach point penalty 4.

Nucleic acid of the present invention and protein sequence can further be used as " search sequence " carrying out the search to public database, thereby as identify other family members or relevant sequence.People such as the available Altschul of this search, the NBLAST of (1990) J.Mol.Biol.215:403-10 and XBLAST program (2.0 editions) are carried out.The BLAST nucleotide search can carry out to obtain and proteolytic enzyme nucleic acid molecule homologous nucleotide sequence of the present invention with NBLAST program, mark=100, word length=12.The BLAST protein search can carry out to obtain and protease protein matter molecule homologous aminoacid sequence of the present invention with XBLAST program, mark=50, word length=3.In order to obtain to be used for the comparison jaggy of comparison purpose, can utilize people such as Altschul (1997) Nucleic Acids Res.25 (17): the Gapped BLAST that describes among the 3389-3402.When utilizing BLAST and Gapped blast program, can use the default parameter of each program (as XBLAST and NBLAST).Referring to http://www.ncbi.nlm.nih.gov.

Hybridization

As used herein, term " hybridization " means describes the condition of hybridizing and washing of being used to, under this condition, be at least about mutually 50%, at least about 60%, at least about 70%, more preferably at least about 80% in addition more preferably at least about 85%～90%, more preferably generally keep the phase mutual cross at least about 95% homologous nucleotide sequence.

A preferred non-limitative example of this hybridization conditions is in 45 ℃ hybridization in the 6X sodium chloride/sodium citrate (SSC), be accompanied by the washing of one or many in 1X SSC, 0.1%SDS, temperature is 50 ℃, preferred 55 ℃, preferred 60 ℃, and be more preferably 65 ℃.

The condition of height stringency comprises as in 68 ℃ of hybridization in 5x SSC/5x Denhardt ' s solution/1.0%SDS, reaches in the washing of room temperature in 0.2x SSC/0.1%SDS.Perhaps, washing can be carried out at 42 ℃.

The technician will know the condition that should use for stringency and height stringency hybridization conditions.Be easy to obtain about the extra guide of this condition from this area, as people such as Sambrook, 1989, molecular cloning: laboratory manual (Molecular Cloning:ALaboratory Manual), Cold Spring Harbor Press, N.Y.; With people (eds.) such as Ausubel, 1995, the current rules of molecular biology (CurrentProtocols in Molecular Biology), (John Wiley ﹠amp; Sons, N.Y.).

Certainly, only with polyadenylic acid sequence (as 3 ' the terminal polyadenylic acid zone of mRNA) or with the polynucleotide of complementary T (or U) residue section hybridization be not included in be used among the present invention specifically with nucleic acid moiety hybridization of the present invention polynucleotide, this is because a kind of like this polynucleotide can be hybridized (as actual what double-stranded cDNA clone that takes up an official post) with any nucleic acid molecule that contains polyadenylic acid section or its complement.

From other biological, obtain the DNA of total length

In general method, can screen the cDNA library that makes up by other biological, as from filamentous fungus, particularly from the Aspergillus species.

For example, aspergillus bacterium can screen homologous proteolytic enzyme polynucleotide by rna blot analysis.To after surveying according to the homeodomain transcription thing of polynucleotide of the present invention, utilize the well-known standard technique of those skilled in the art can be from separating from the RNA of suitable strain construction cDNA library.Perhaps, total genome dna library can screen with the probe with proteolytic enzyme multi-nucleotide hybrid according to the present invention.

For example, the homologous gene sequence can be separated by carrying out PCR, two Oligonucleolide primers that this PCR use designs on described nucleotide sequence basis or the Oligonucleolide primers storehouse of two degeneracys.

The template of reaction can be by mRNA is carried out the cDNA that reverse transcription obtains, and this mRNA prepares from known or conjecture can be expressed bacterial classification according to polynucleotide of the present invention.Can carry out subclone to the PCR product and represent the sequence of nucleotide sequence or its function equivalent of new proteolytic enzyme with the sequence of guaranteeing to increase with checking order.

The PCR fragment can be used to separate the cDNA clone of total length by multiple known method then.For example, can carry out mark and be used to screen phage or clay cDNA library the fragment of amplification.Perhaps, the fragment of mark can be used for screening-gene group library.

Also round pcr can be used for separating full length cDNA sequence from other biological.For example, according to standard program, can be from suitable cell or tissue source isolation of RNA.Available Oligonucleolide primers carries out reverse transcription reaction to cause the synthetic of first chain on RNA, Oligonucleolide primers wherein is specific to 5 ' end of most of institutes amplified fragments.

The RNA/DNA hybrid of gained as a result can be carried out " tailing (tailed) " (as use guanine) with the terminal enzyme (DNA) reaction of standard then, this hybrid can digest with RNase H, and can cause second chain synthetic (as with gathering the cytosine(Cyt) primer) then.Thereby the cDNA sequence of institute amplified fragments upstream can be easy to separate.The summary of useful clone's strategy is referring to as people such as Sambrook, on seeing; People such as Ausubel are on seeing.

Carrier

Another aspect of the present invention is preferably expression vector about carrier, and this carrier contains the nucleic acid of proteins encoded zymoprotein or its function equivalent.As used herein, term " carrier " refers to transport the nucleic acid molecule of another nucleic acid that is connected with it.One class carrier is " plasmid ", and it refers to can connect therein the circular double-stranded DNA ring of extra DNA section.Another kind of carrier is a virus vector, and wherein extra DNA section can connect into viral genome.Some carrier can be in the host cell that they are introduced the self-replicating bacteria carrier and the additive type Mammals carrier of bacterium replication orgin (as have).Other carriers (as non-add type Mammals carrier) will be integrated into the host cell gene group after in being incorporated into host cell, and duplicate with host genome whereby.In addition, some carrier can instruct the expression of gene that operationally is connected with them.This carrier is called " expression vector " herein.Usually, the expression vector that utilizes in recombinant DNA technology often adopts the form of plasmid.Term " plasmid " and " carrier " are used interchangeably herein, and this is because plasmid is the most frequently used form of carrier.Yet the present invention means and comprises other forms of expression vector, as the virus vector (as replication defect type retrovirus, adenovirus (adenoviruses) and adeno associated virus (adeno-associated viruses)) of performance equivalent functions.

Recombinant expression vector of the present invention is to be suitable for comprising nucleic acid of the present invention in the form of host cell inner expression nucleic acid, this means that recombinant expression vector is included in one or more adjusting sequences of selecting on the host cell basis that is used to express, this adjusting sequence is operably connected on the nucleotide sequence that will express.In recombinant expression vector, the meaning that " is operably connected " feeling the pulse with the finger-tip mark nucleotide sequence is so that the mode that nucleotide sequence can be expressed is connected to and regulates on the sequence (as in in-vitro transcription/translation system or in carrier is incorporated into host cell the time, in host cell).Term " adjusting sequence " means and comprises promotor, enhanser and other expression controlling elementss (as polyadenylation signal).This adjusting sequence description is in as Goeddel; Gene expression technique: Enzymology method (Gene Expression Technology:Methods inEnzymology) 185, Academic Press, San Diego, CA (1990).That regulates constitutive expression that sequence comprises that those instruct nucleotide sequence in many type host cells regulates sequence with those (as tissue-specific) of only instructing nucleotide sequence to express in some host cell.The technician should be appreciated that the design of expression vector can be dependent on as factors such as the selection of wanting transformed host cells, desired protein expression levels.Expression vector of the present invention can be incorporated in the host cell to produce protein or the peptide (as protease protein matter, the mutant of protease protein matter, its fragment, variant or function equivalent, fused protein etc.) by described nucleic acid encoding whereby.

Recombinant expression vector of the present invention can be protokaryon or eukaryotic cell endoproteinase protein expression and designs.For example, protease protein matter can be expressed in bacterial cell such as intestinal bacteria, insect cell (using baculovirus (baculovirus) expression vector), yeast cell or mammalian cell.Appropriate host cell is further described in Goeddel, gene expression technique: Enzymology method (Gene Expression Technology:Methods inEnzymology) 185, Academic Press, San Diego, CA (1990).Perhaps, recombinant expression vector can for example be regulated sequence and T7 polysaccharase with the T7 promotor in in-vitro transcription and translation.

Being used for expression vector of the present invention comprises and is derived from chromosomal, additive type and carrier virus, as be derived from bacterial plasmid, phage, yeast episome, yeast chromosomal element, virus as baculovirus, papovavirus (papova viruses), vaccinia virus (vaccinia viruses), adenovirus, fowlpox virus (fowl poxviruses), pseudorabies virus (pseudorabies viruses) and retrovirus, with the carrier that is derived from its combination, be derived from the carrier of plasmid and phage genetic elements as those, as clay and phagemid.

DNA inserts fragment and should be operably connected on the suitable promotor, as phage PL promotor, intestinal bacteria lac, trp and tac promotor, SV40 is early stage and late promoter and retrovirus LTRs promotor etc.Other suitable promotors are that the technician is known.In a specific embodiment, preferably those can instruct the promotor of proteolytic enzyme high level expression in the filamentous fungus.This promotor is known in this field.This expression construct can contain transcription initiation, terminated site and transcribe and is used to the ribosome bind site translated in the zone.The encoding part of the mature transcript of being expressed by construct will comprise translation initiation AUG in the initial of the polypeptide that will translate, and comprise suitable localized terminator codon at its end.

Carrier DNA can be incorporated into protokaryon or eukaryotic cell by the conversion or the rotaring dyeing technology of routine.As used herein, term " conversion " and " transfection " mean the multiple art-recognized technology that exogenous nucleic acid (as DNA) is incorporated into host cell, comprise transfection, transduction, infection, the fat transfection of calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediation, the transfection or the electroporation of cation lipid mediation.But transform and the proper method of transfection host cell it in people such as Sambrook (molecular cloning: laboratory manual (Molecular Cloning:ALaboratory Manual), 2 ^NdEd., Cold Spring HarborLaboratory, Cold Spring Harbor Laboratory Press, ColdSpring Harbor, NY, 1989), people such as Davis, molecular biology basic skills (Basic Methods in Molecular Biology) (1986) and other laboratory manuals.

For the stable transfection of mammalian cell, depend on used expression vector and rotaring dyeing technology, known only have a fraction of cell foreign DNA can be integrated into its genome.In order to identify and select these intasomies, the gene (as antibiotics resistance) with a coding selected marker is incorporated in the host cell with target gene usually.Preferred selected marker comprises that those give medicine such as G418, Totomycin and methotrexate (mark of resistance).The nucleic acid of coding selected marker can be introduced host cell on the carrier identical with the proteins encoded zymoprotein, perhaps can introduce on independent carrier.Can identify (that is: the cell that has inserted the selected marker can be survived, and other cells are then dead) by medicament selection by the nucleic acid stability cells transfected of introducing.

Protein expression is realized with the carrier that contains composing type or inducible promoter in the intestinal bacteria of being everlasting in the prokaryotic organism, and this promotor instructs the expression of fusion or non-fused protein.Fused protein joins many amino acid in the encoded protein matter, as joins the N-terminal of recombinant protein.This fusion vector is generally as three purposes: 1) increase the expression of recombinant protein; 2) solvability of increase recombinant protein; With 3) help purifying recombinant proteins by in affinity purification, serving as part.In fusion expression vector, the contact place that the proteolysis cleavage site merges part and recombinant protein through being everlasting introduces so that behind the fused protein purifying, and recombinant protein can separate from merging part.This kind of enzyme and relevant recognition sequence thereof comprise factor Xa, zymoplasm and enteropeptidase.

As shown, expression vector will preferably contain selected marker.This mark comprises the Tetrahydrofolate dehydrogenase of eukaryotic cell culture or the tsiklomitsin or the amicillin resistance of neomycin resistance and intestinal bacteria and other microbial culture.Suitably host's representative example comprises bacterial cell, as intestinal bacteria, streptomyces (Streptomyces) and Salmonella typhimurium (Salmonella typhimurium); Fungal cell such as yeast; Insect cell such as fruit bat S2 and noctuid Sf9; Zooblast such as CHO, COS and Bowes melanoma; And vegetable cell.The suitable culture medium of above-mentioned host cell and condition are known in this field.

In carrier, preferred pQE70, pQE60 and the PQE-9 of using in the bacterium can be available from Qiagen; PBS carrier, Phagescript carrier, Bluescript vector, pNH8A, pNH16A, pNH18A, pNH46A can be available from Stratagene; With can be available from ptrc99a, pKK223-3, pKK233-3, pDR540, the pRIT5 of Pharmacia.Preferred eukaryotic vector has can be available from PWLNEO, pSV2CAT, pOG44, pZT1 and the pSG of Stratagene; With can be available from PSVK3, pBPV, pMSG and the pSVL of pharmacia.Other appropriate carriers are apparent for the technician.

Comprise that intestinal bacteria lacI and lacZ promotor, T3 and T7 promotor, gpt promotor, λ PR, PL promotor and trp promotor, HSV thymidine kinase promoter, early stage and late period SV40 promotor, retrovirus LTRs promotor are as from Rous sarcoma virus (Rous sarcoma virus) (" RSV ") and metallothionein promoter such as mouse metallothionein(MT)-I promotor being used for known bacterium promotor of the present invention.

The DNA of code book invention polypeptide transcribing in more high eukaryote can increase by insert enhancer sequence in carrier.Enhanser is the cis-acting elements of DNA, and it is long to be generally about 10～300bp, act as the transcriptional activity that increases promotor in given host cell type.The example of enhanser comprises SV40 enhanser, cytomegalovirus (cytomegalovirus) early promoter enhanser, the polyoma enhanser that is positioned at replication orgin side in late period and the adenovirus enhanser of bp100～270 of the late side that is positioned at replication orgin.

For with translated protein in the chamber of endoplasmic reticulum, to pericentral siphon zone or in extracellular environment, secrete, suitable secretion signal can be inserted in the polypeptide expressed.This signal can be endogenous to polypeptide, perhaps they can be allogenic signal.

The formal representation that polypeptide can have been modified as fused protein, and can not only comprise secretion signal, and comprises extra allos functional zone.Thereby, for example can be with an extra amino acid, the N-end that particularly charged amino acid joins polypeptide with improve this polypeptide purifying or in subsequently processing and storage stability and the time length in host cell.Similarly, peptide moiety can be joined in the polypeptide to promote purifying.

According to polypeptide of the present invention

The invention provides a kind of isolated polypeptide, this polypeptide has the aminoacid sequence that is selected from SEQ IDNO:115～SEQ ID NO:171, obtainable or express the obtainable aminoacid sequence of sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 in a preferred embodiment in suitable host by expressing polynucleotide according to the present invention, and by in suitable host, expressing the obtainable aminoacid sequence of polynucleotide sequence that is selected from SEQ ID NO:58～SEQ ID NO:114.Similarly, peptide or the polypeptide that comprises the function equivalent of aforementioned polypeptides is contained among the present invention.Aforementioned polypeptides all is contained in the term " according to polypeptide of the present invention ".

Term " peptide " and " oligopeptides " are considered to synonym (being familiar with as common), and required as situation, and each term all uses with indication interchangeably by two amino acid whose chains of peptide base key link coupled at least.Word " polypeptide " is herein as containing the chain that surpasses 7 amino-acid residues.Oligopeptides herein and peptide molecule formula or sequence all from left to right and in the direction from the N-terminal to the C-terminal are write.Amino acid one-letter code used herein is known in this field, and is found in people's (molecular clonings: laboratory manual (MolecularCloning:A Laboratory Manual), 2 such as Sambrook ^Nd, ed., Cold Spring HarborLaboratory, Cold Spring Harbor Laboratory Press, ColdSpring Harbor, NY, 1989) in.

" isolating " polypeptide or protein mean polypeptide or the protein that takes out from its natural surroundings.For example, it is isolating that the polypeptide of the recombinant production of expressing in host cell and protein are considered to purpose according to the present invention, as natural or recombinant polypeptide by many suitable basic purifying of technology, this technology is as at Smith and Johnson, disclosed single stage purification process among the Gene 67:31-40 (1988).

Can reclaim and purifying from the reconstitution cell culture with well-known method according to proteolytic enzyme of the present invention, this method comprises ammonium sulfate or ethanol sedimentation, acid extraction, negatively charged ion or cation-exchange chromatography, phosphorylated cotton chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite (hydroxylapatite) chromatography and lectin chromatography.For analysis purposes most preferably high performance liquid chromatography (" HPLC ") be used for purifying.

Polypeptide of the present invention comprises the product of natural purifying, the product of chemosynthesis program and the product of being produced by recombinant technology from protokaryon or eucaryon host, this host comprises as bacterium, yeast, higher plant, insect and mammalian cell.Depend on the host who adopts in the recombinant production process, polypeptide of the present invention can glycosylation or non-glycosylated.In addition, as the result of the process of host mediation, polypeptide of the present invention also can comprise the methionine residues of initial modification in some cases.

In addition, protein according to the present invention can be precursor protein matter such as proenzyme, hybridization protein, as the protein of presequence or the former acquisition of presequence, the perhaps non-mature form of any other type.

Protein fragments

The present invention has also described the biological active fragment according to polypeptide of the present invention.

The biological active fragment of polypeptide of the present invention comprises that the aminoacid sequence that comprises with protease protein matter has sufficient consistence or is derived from the polypeptide of latter's's aminoacid sequence of SEQ ID NO:115～SEQ ID NO:171 sequence (as be selected from) aminoacid sequence, it comprises the amino acid that lacks than full length protein, and shows the biologic activity of at least a corresponding full length protein.Usually, biological active fragment comprises and has at least a protease protein matter active structures territory or primitive.It is 10,25,50,100 or the polypeptide of amino acids more that proteinic biological active fragment of the present invention can be as length.In addition, other biological is learned one or more biologic activity that active part can prepare and assess its polypeptide natural form of the present invention that has by recombinant technology, and wherein proteinic other zones lack.

The present invention has also described the nucleic acid fragment of the biological active fragment of the above-mentioned protease protein matter of encoding.

Fused protein

Protein of the present invention or its function equivalent such as its biologic activity partly can be operatively attached to non-protease polypeptide (as the allogeneic amino acid sequence) and go up to form fused protein.As used herein, " chimeric protein " or " fused protein " of proteolytic enzyme comprise the protease polypeptide that is operably connected on the non-protease polypeptide." protease polypeptide " refers to have corresponding to the polypeptide according to the aminoacid sequence of peptide sequence of the present invention, and " non-protease polypeptide " refers to have the polypeptide corresponding to a proteinic aminoacid sequence, protein wherein substantially not with protein homology according to the present invention, as be different from protein protease protein matter or that be derived from identical or different biology.In a proteolytic enzyme fused protein, protease polypeptide can be corresponding to according to of the present invention proteinic all or part of.In a preferred embodiment, the proteolytic enzyme fused protein comprises at least one according to proteinic biological active fragment of the present invention.In another preferred embodiment, the proteolytic enzyme fused protein comprises at least two according to proteinic biological active fragment of the present invention.In fused protein, term " is operably connected " and means protease protein matter and non-protease protein matter merges mutually to meet the mode of reading frame.Non-protease polypeptide can be fused to the N-end or the C-end of protease polypeptide.

For example, in one embodiment, fused protein GST-proteolytic enzyme fused protein, wherein the proteolytic enzyme sequence is fused to the C-end of GST sequence.This fused protein can promote the purifying of recombinant protein enzyme.In another embodiment, fused protein is a kind of protease protein matter that contains the allos signal sequence at its N-end.In some host cell (as Mammals and yeast host cell), the expression of proteolytic enzyme and/or secretion can increase by using the allos signal sequence.

In another example, the gp67 secretion sequence of baculovirus envelope protein can be used as allos signal sequence (the current rules of molecular biology (Current Protocols inMolecular Biology), people such as Ausubel, eds., John Wiley ﹠amp; Sons, 1992).Other examples of eucaryon allos signal sequence comprise the secretion sequence (Stratagene of mellitin and placental alkaline phosphatase; La Jolla, California).In the another one example, useful protokaryon allos signal sequence comprises phoA secretion signal (people such as Sambrook is on seeing) and a-protein secretion signal (Pharmacia Biotech; Piscataway, New Jersey).

The secretion that signal sequence can be used to promote protein of the present invention or polypeptide with separate.The signal sequence general feature is the core of a hydrophobic amino acid, this core usually in secretion process through one or repeatedly cut incident and from mature protein, excise.The sort signal peptide contains the processing site, and this site makes signal sequence can excise from mature protein through Secretory Pathway the time.Signal sequence instructs proteinic secretion, and as secreting from the eucaryon host that this expression vector transformed, and signal sequence is cut subsequently or simultaneously.This protein can be easy to art-recognized method purifying from the substratum of extracellular then.Perhaps, this signal sequence can be connected on the target protein with the sequence that promotes purifying, as has the sequence of GST structural domain.Thereby, for example the sequence of coded polypeptide can be fused on the flag sequence, as the sequence of encoded peptide, this sequence promotes the purifying of fusion polypeptide.In some embodiment preferred of this respect of the present invention, flag sequence is six Histidine peptides, as the mark that in the pQE carrier, provides (Qiagen, Inc.), wherein many can buying by commodity.For example, as people such as Gentz, described in the Proc.Natl.Acad.Sci.USA 86:821-824 (1989), six Histidines provide the purifying that makes things convenient for to fused protein.The HA mark is another peptide that is used for purifying, and this peptide is corresponding to the epitope that is derived from influenza hemagglutinin protein matter, and this is by as people such as Wilson, and Cell 37:767 (1984) is described.

Preferably, the chimeric or fused protein of proteolytic enzyme of the present invention is by the recombinant DNA technology production of standard.For example, link together to meet the mode of reading frame according to will the encode dna fragmentation of different peptide sequences of routine techniques, for example by the end that adopts blunt end or staggered end connect, adopt restriction enzyme digestion provide suitable end, suitably mend flat sticky end, adopt alkaline phosphatase treatment to avoid the connection of unwanted connection and enzymatic.In another embodiment, fused protein can synthesize by routine techniques, and this technology comprises automatic dna synthesizer.Perhaps, pcr amplification to gene fragment can be implemented with anchor primer, this primer can cause the complementary overhang between two successive gene fragments, it can be annealed subsequently and increase again to generate chimeric gene sequence (referring to as the current rules of molecular biology (Current Protocols in Molecular Biology), people such as Ausubel, John Wiley ﹠amp; Sons:1992).In addition, many expression vectors that merge part of having encoded can be buied (as gst polypeptide) by commodity.The protease-encoding nucleic acid clone can be gone in such expression vector, thereby this fusion part is connected on the protease protein matter to meet the mode of reading frame.

Function equivalent

Term " function equivalent " and " functional variant " are used interchangeably herein.Function equivalent according to DNA of the present invention is the separated DNA fragment of coded polypeptide, and this polypeptide is showed as the special function of defined aspergillus niger proteolytic enzyme herein.According to the function equivalent of polypeptide of the present invention is to show as the polypeptide of at least one function of defined aspergillus niger proteolytic enzyme herein.

Functional protein or polypeptide Equivalent can only contain and a plurality of amino acid whose conservative substituting of the sequence that is selected from SEQ ID NO:115～SEQ IDNO:171, and perhaps non-primary amino acid substitutes, inserts or disappearance.Therefore, non-primary amino acid is can change in the sequence that is selected from SEQ IDNO:115～SEQ ID NO:171 and significantly do not change the residue of biological function.For example, conservative amino-acid residue is estimated should not change especially in protease protein matter of the present invention.In addition, conservative unlikely the suiting of amino acid changes in protease protein matter according to the present invention and other proteolytic enzyme.

Term " conservative substituting " means wherein amino-acid residue substituting with the radical amino acid replacement with similar side chain.These families are as known in the art, and comprise having basic side chain (as Methionin, arginine and Histidine), acid side-chain is (as aspartic acid, L-glutamic acid), uncharged polar side chain is (as glycine, l-asparagine, glutamine, Serine, Threonine, tyrosine, halfcystine), non-polar sidechain is (as L-Ala, Xie Ansuan, leucine, Isoleucine, proline(Pro), phenylalanine, methionine(Met), tryptophane), β-branch side chain is (as Threonine, Xie Ansuan, Isoleucine) and aromatic side chains (as tyrosine, phenylalanine, tryptophane, Histidine) amino acid.

The functional nucleic acid Equivalent generally contains silent mutation or does not change the sudden change of the biological function of coded polypeptide.Therefore, the invention provides the nucleic acid molecule of proteins encoded zymoprotein, this protein contains the change for the nonessential amino-acid residue of concrete biologic activity.This protease protein matter is variant in aminoacid sequence with the sequence that is selected from SEQ ID NO:115～SEQ ID NO:171, but still has kept at least a biologic activity.In one embodiment, isolated nucleic acid molecule comprises the nucleotide sequence of coded protein, wherein this protein comprise with the aminoacid sequence that in the sequence that is selected from SEQ ID NO:115～SEQ ID NO:171, shows at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% homology or the basic homologous aminoacid sequence of homologous more.

For example, about the guide of the amino acid replacement that how to prepare the phenotype silence at Bowie, people such as J.U., Science 247:1306-1310 provides in (1990), wherein the author shows two kinds of main methods and is used to study the tolerance of aminoacid sequence to changing.First method depends on evolutionary process, wherein suddenlys change to be accepted by natural selection or discarded.Second method utilizes genetically engineered to change to introduce amino acid at the specific position of cloned genes, and selects or screening is kept functional sequence with evaluation.As author's statement, these researchs disclose protein and have surprising tolerance for amino acid replacement.The author further is presented at which kind of variation of proteinic some position may be allowed.For example, most of hidden amino-acid residues require non-polar sidechains, and seldom the feature of surface side chains is guarded usually.Other this phenotype silences alternative is described in people such as Bowie, sees in the reference of going up and quoting.

Introduce one or more nucleotide substitutions, increase or disappearance in the coding nucleotide sequence of the isolated nucleic acid molecule of coding and the protease protein matter of the protein homology that is selected from SEQ ID NO:115～SEQ ID NO:171 can be by being selected from sequence from SEQ ID NO:1～SEQID NO:57 to basis or the sequence that is selected from SEQ ID NO:58～SEQ ID NO:114 and form, thereby one or more amino acid replacement, disappearance or insertion promptly are incorporated in the encoded protein matter.This sudden change can be introduced by standard techniques, as site-directed mutagenesis and PCR-mediated mutagenesis.

Term " function equivalent " also comprise aspergillus niger protease protein matter directly to homologue (orthologues).The straight of aspergillus niger protease protein matter is the protein that can separate and have similar or identical biologic activity from other strains or species to homologue.Thisly directly can be easy to be accredited as the basic homologous aminoacid sequence of sequence that comprises and be selected from SEQ ID NO:115～SEQ ID NO:171 to homologue.

As defined herein, term " basic homology " refers to that first amino acid or nucleotide sequence contain amino acid or Nucleotide enough or minimum purpose identical or of equal value with second amino acid or nucleotide sequence (as having similar side chain), thereby first has the common structural domain with second amino acid or nucleotide sequence.For example, contain have about 60%, be preferably 65%, more preferably be 70%, more preferably be 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% 99% or the amino acid or the nucleotide sequence in more conforming common structure territory be defined as enough identical herein.

Equally, encode other proteolytic enzyme family member nucleic acid within the scope of the present invention, this nucleic acid thereby have with the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or be selected from the different nucleotide sequence of sequence of SEQ ID NO:58～SEQ ID NO:114.In addition, coding from the nucleic acid of the protease protein matter of other species within the scope of the present invention, this nucleic acid thereby have with the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or be selected from the different nucleotide sequence of sequence of SEQ IDNO:58～SEQ ID NO:114.

Can be corresponding to the nucleic acid molecule of the variant (as natural allele variant) of albumen enzyme dna of the present invention and homologue based on it with the homology of disclosed proteolytic enzyme nucleic acid herein and separate, preferably under height stringency hybridization conditions, be used in cDNA disclosed herein or its suitable fragment as hybridization probe according to the hybridization technique of standard.

Except that the naturally occurring allele variant of proteolytic enzyme sequence, the technician will recognize in the nucleotide sequence of the sequence that can be by sudden change variation be incorporated into the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or be selected from SEQ ID NO:58～SEQ ID NO:114, cause the variation in the aminoacid sequence of protease protein matter whereby and significantly not change the function of protease protein matter.

In another aspect of the present invention, provide improved protease protein matter.Improved protease protein matter is the protein that wherein at least a biologic activity is improved.This protein can obtain by introduce sudden change randomly on the protease-encoding sequence of all or part, as by saturation mutagenesis, and the mutant of the ground expression of results gained of can recombinating and screen its biologic activity.For example, this area provides the standard test of the enzymic activity that is used to measure proteolytic enzyme, thereby can be easy to select improved protein.

In a preferred embodiment, protease protein matter has according to the aminoacid sequence that is selected from the sequence of SEQ IDNO:115～SEQ ID NO:171.In another embodiment, protease polypeptide with according to the basic homology of aminoacid sequence of the sequence that is selected from SEQ ID NO:115～SEQ ID NO:171 and keep according at least one biologic activity that is selected from the polypeptide of sequence of SEQ ID NO:115～SEQ IDNO:171, but because natural variation or above-mentioned mutagenesis and variant in aminoacid sequence.

In a further preferred embodiment, protease protein matter has by isolating nucleic acid fragment amino acid sequence coded, and this isolating nucleic acid fragment can be preferably hybridized under height stringency hybridization conditions with according to the nucleic acid that is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from the sequence of SEQID NO:58～SEQ ID NO:114.

Therefore, protease protein matter is to comprise with the aminoacid sequence that shows in the sequence that is selected from SEQ ID NO:115～SEQ IDNO:171 to have at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or the protein of the aminoacid sequence of bigger homology, and has kept according at least a functionally active that is selected from the polypeptide of sequence of SEQ ID NO:115～SEQ IDNO:171.

Also can identify according to proteinic function equivalent of the present invention as the protease activity of the combinatorial library of the mutant by the screening proteinic mutant of the present invention such as brachymemma.In one embodiment, various variant library forms by the combinatorial mutagenesis on nucleic acid level.Various variation library can be expressed as independent polypeptide or one group of bigger fused protein (as being used for phage display) thereby one of potential protein sequence is overlapped the degeneracy group by producing as synthetic oligonucleotide mixture enzymatic is connected in the gene order.There are many methods to can be used for from the oligonucleotide sequence of degeneracy, producing the potential variant library of polypeptide of the present invention.The method of synthetic degenerate oligonucleotide is known in the art (referring to as Narang (1983) Tetrahedron39:3; People such as Itakura, (1984) Annu.Rev.Biochem.53:323; People such as Itakura, (1984) Science 198:1056; People such as Ike, (1983) Nucleic Acid Res.11:477).

In addition, the fragment library of the encoding sequence of polypeptide of the present invention can be generated and be used to screen follow-up variant selects diversified polypeptide colony.For example, the segmental library of encoding sequence can form by following method, each molecule only take place under the condition of an about otch (nicking) with nuclease processing target encoding sequence double-stranded PCR fragment, make this double-stranded DNA sex change, make the DNA renaturation can comprise from difference and incise the right double-stranded DNA of justice/antisense is arranged, partly and with the fragment of gained as a result connecting into the expression vector of product by handling with the S1 nuclease from the duplex removal strand of reconstruct to form.By this method, the expression library of the interior segments of the terminal and multiple size of the N-of the target protein that can obtain encoding.

Be known in the art several technology and be used for, and have the gene product of selected character from the cDNA library screening from combinatorial library screening-gene product by the point mutation of truncated mutant preparation.The most widely used technology that is suitable for the big gene library of screening of high throughput analysis generally comprises gene library is cloned in the rf expression vector, transforms suitable cell and express this combination gene under certain condition with the vector library of gained as a result, and active detection has promoted coding is detected the separation of the carrier of gene product to purpose in this condition.A kind of technology that strengthens function mutation body frequency in the library returns whole mutagenesis (recursive ensemblemutagenesis) and (REM) can be used from the proteinic variant of evaluation the present invention (Arkin and Yourvan (1992) Proc.Natl.Acad.Sci.USA89:7811-7815 with this screening assay one; People such as Delgrave, (1993) Protein Engineering 6 (3): 327-331).

Except that the proteinase gene sequence that in the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57, shows, for those skilled in the art, it is evident that in given colony to have the dna sequence dna polymorphism, this polymorphism can cause the variation of aminoacid sequence in the protease protein matter.This genetic polymorphism can be present in from the cell of different groups or because in the cell in the colony of natural allelic variation.Allelic variation also can comprise function equivalent.

Fragment according to polypeptide of the present invention also can comprise the not polynucleotide of encoding function polypeptide.This polynucleotide can be brought into play the function of the primer of probe or PCR reaction.Also available this polynucleotide (gene knockout mutant) when wanting the functionally active of destruction proteolytic enzyme in concrete organism.

No matter according to their encoding functions of nucleic acid of the present invention or non-functional polypeptide, all can be used as hybridization probe or polymerase chain reaction (PCR) primer.The purposes of nucleic acid molecule of the present invention of polypeptide with protease activity of not encoding comprises that in addition (1) is from for example from gene or its allelic mutant of separating the proteins encoded zymoprotein the cDNA library of the organism except that aspergillus niger; (2) to the in situ hybridization (as FISH) of Metaphase Chromosome expansion so that the accurate location of proteinase gene in karyomit(e) to be provided, as be described in people such as Verma, human chromosome: basic fundamental handbook (Human Chromosomes:a Manual of BasicTechniques), Pergamon Press, the method among the New York (1988); (3) whether the rna blot analysis probe that can be used as diagnostic tool with the expression of surveying proteolytic enzyme mRNA in particular organization and/or cell and (4) and primer can exist with the nucleic acid of proteolytic enzyme probe hybridization in given biology (as tissue) sample to analyze.

The present invention comprises the method for the function equivalent that obtains proteinase gene or cDNA equally.This method need obtain the probe of mark, and this probe comprises that coding is according to the isolating nucleic acid or its variant that are selected from the SEQ IDNO:115～sequence of SEQ ID NO:171 or all or part of sequence of its variant; Probe with mark under the condition that nucleic acid fragment in probe and library can be hybridized screens the nucleic acid fragment library, form nucleic acid duplex whereby, and prepare the gene order of total length to obtain the gene relevant with proteinase gene from the nucleic acid fragment of the duplex of any mark.

In one embodiment, proteolytic enzyme nucleic acid of the present invention with in the sequence that is selected from SEQ IDNO:1～SEQ ID NO:57, be selected from SEQ ID NO:58～sequence of SEQ ID NO:114 or the nucleotide sequence of its complement sequence and have at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or bigger homology.

In another preferred embodiment, protease polypeptide of the present invention with have at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or bigger homology at the aminoacid sequence that is selected from SEQID NO:115～SEQ ID NO:171 sequence.

Host cell

In another embodiment, the invention describes the cell that contains nucleic acid of the present invention, as transformed host cells or recombinant host cell." cell transformed " or " cell of reorganization " is to rely on recombinant DNA technology to introduce the cell according to nucleic acid of the present invention to wherein (or in its ancestors).This comprises protokaryon and eukaryotic cell, as bacterium, fungi, yeast etc., especially preferably from the filamentous fungus cell of aspergillus niger particularly.

Can select to regulate the expression of insertion sequence or modify host cell with the processed gene product in specific required mode.This modification to protein (as glycosylation) and processing (as cutting) can promote the suitableeest proteinic function performance.

Multiple host cell has translates the distinctive and specific mechanism of post-treatment and modification to protein and gene product.Can select the technician is familiar with in molecular biology and/or the microbiology field suitable clone or host system to guarantee that the exogenous protein of expressing is carried out required and correct modification and processing.For this purpose, can use the eukaryotic host cell that gene product is carried out correct primary transcript processing, glycosylation and phosphorylation.This host cell is well-known in this area.

Host cell is also including, but not limited to mammal cell line such as CHO, VERO, BHK, HeLa, COS, MDCK, 293,3T3, WI38 and choroid plexus cell system.

If desired, can produce by the clone of stable transfection according to polypeptide of the present invention.Many carriers that are suitable for the stable transfection of mammalian cell are public obtainable, and the method that makes up this clone also is known technology, as described in the people such as Ausubel (on seeing).

Antibody

The present invention has further described specificity in conjunction with the antibody according to protease protein matter of the present invention, as mono-clonal or polyclonal antibody.

As used herein, term " antibody " (Ab) or " monoclonal antibody " (Mab) refer to comprise can the binding proteins specific zymoprotein complete molecule and antibody fragment (as Fab and F (ab ') ₂Fragment).Fab and F (ab ') ₂Fragment lacks the Fc fragment of complete antibody, can remove quickly in circulation, and can have the non-specific tissue bond lacked than complete antibody people such as (, J.Nucl.Med.24:316-325 (1983)) Wahl.Thereby, preferred these fragments.

Antibody of the present invention can prepare by any of several different methods.For example, expressing protein zymoprotein or the segmental cell of its antigenicity can be applied to animal to induce the production of the serum that contains polyclonal antibody.In a preferable methods, protease protein matter prepared product is prepared with purifying so that it does not contain natural pollutent substantially.This preparation is incorporated in the animal then and has the more active polyclonal antiserum of high specific with production.

In most preferred method, antibody of the present invention is monoclonal antibody (or its protease protein matter binding fragment).This monoclonal antibody can prepare (people such as Kohler, Nature 256:495 (1975) with hybridoma technology; People such as Kohler, Eur.J.Immunol.6:511 (1976); People such as Hammerling, In: monoclonal antibody and T-quadroma (Monoclonal Antibodies and T-Cell Hybridomas), Elsevier, N.Y., pp.563-681 (1981)).Usually, this program comprises with protease protein matter antigen or with protease protein matter express cell animal (being preferably mouse) is carried out immunization.The splenocyte of this mouse extracted and merge with suitable myeloma cell line.Can adopt any suitable myeloma cell line according to the present invention; Yet, preferably adopt parent myeloma cell line (SP ₂O), available from American Type Culture Collection (American Type Culture Collection), Rockville, Maryland.After the fusion, the hybridoma of gained as a result optionally is maintained on the HAT substratum, clones by restrictive dilution then, such as by people such as Wands (Gastro-enterology 80:225-232 (1981)) description.To measure clone by the hybridoma that this selection obtains then to identify that secretion can the antigenic antibody of conjugated protein zymoprotein.Usually, this polypeptide can with carrier protein such as KLH coupling, as described in people such as Ausubel, on seeing, mix with adjuvant, and be expelled in the host mammal.

Particularly, multiple host animal can carry out immunization by the injection to target polypeptides.The example of suitable host animal comprises rabbit, mouse, cavy and rat.Depend on host species, multiple adjuvant can be used for increasing immune response, and this adjuvant is including, but not limited to Fu Shi (fully with incomplete), adjuvant mineral glue such as aluminium hydroxide, surfactant such as lysolecithin, polyol, polyanion, peptide, emulsifier, keyhole limpet hemocyanin, dinitrophenol(DNP), BCG (bacille Calmette-Guerin vaccine) and spillikin bacillus (Corybebacterium parvum).Polyclonal antibody is the heterogeneous population of antibody molecule that is derived from the serum of immunization animal.

This antibody can be any immunoglobulin (Ig) kind and the subclass thereof that comprises IgG, IgM, IgE, IgA, IgD.The hybridoma of producing mAb of the present invention can be external or cultivate in vivo.

In case produced, promptly in immunoassay such as western blotting or immunoprecipitation analysis, check polyclone or monoclonal antibody to the specific recognition of protease polypeptide or its function equivalent with standard technique, describe as people such as Ausubel, on seeing.The antibody of binding proteins specific zymoprotein or its function equivalent can be used among the present invention.For example, this antibody can be used in the immunoassay to survey the proteolytic enzyme (as in the aspergillus extract) in pathogenic or the non-pathogenic aspergillus bacterial classification.

Preferably, antibody of the present invention is with the fragment production of protease polypeptide, and the standard that this fragment charged residue occurs with for example high frequency may be shown as has antigenicity.For example, this fragment can generate by the standard round pcr, is cloned into then in the pGEX expression vector people such as (, on seeing) Ausubel.Fused protein can be at expression in escherichia coli then, and carries out purifying with the glutathione agarose affinity matrix, describes as people such as Ausubel, on seeing.If want, can generate several (as two or three) to each protein and merge, and each fusion injectable is gone in two rabbits at least.Antiserum(antisera) can cause by series of injections, generally comprises at least three booster shots.Usually, detect the ability of antiserum(antisera) immunoprecipitation recombinant protein enzyme polypeptide or its function equivalent, and incoherent protein can serve as the specific contrast of immune response.

Perhaps, can adopt described single-chain antibody production technology (United States Patent (USP) 4,946,778 and 4,704,692) to produce the single-chain antibody of protease inhibitor polypeptide or its function equivalent.Generate and the test kit of screening phage display library can be available from as Pharmacia.

In addition, it is visible as U.S. Patent No. 5,223,409 specifically to be suitable for generating and screen the method and the reagent of antibody display libraries; The open text No.WO 92/18619 of PCT; The open text No.WO 91/17271 of PCT; The open text No.WO 20791 of PCT; The open text No.WO 92/20791 of PCT; The open text No.WO 92/15679 of PCT; The open text No.WO 93/01288 of PCT; The open text No.WO 92/01047 of PCT; The open text No.WO 92/09690 of PCT; The open text No.WO 90/02809 of PCT; People such as Fuchs (1991) Bio/Technology 9:1370-1372; People such as Hay (1992) Hum.Antibod.Hybridomas 3:81-85; People such as Huse (1989) Science 246:1275-1281; People such as Griffiths (1993) EMBO J.12:725-734.

The polyclone of binding proteins specific enzyme polypeptide or its function equivalent and monoclonal antibody can be used for as surveying proteinase gene or its as expression at the function equivalent of other bacterial classifications of Aspergillus.

For example, protease polypeptide can be easy to survey in the conventional determining to Aspergillus cell or extract.The example of suitable detection comprises western blotting, ELISA, radioimmunoassay etc. without limitation.

" specifically in conjunction with " meaning refers to antibody recognition and in conjunction with special antigen, as protease polypeptide, but other irrelevant molecules in basic nonrecognition and the debond sample.

Antibody can be by carrying out purifying as the affinity chromatography method, and wherein polypeptide antigen is fixed on the resin.

Can be with resisting the antibody (as monoclonal antibody) of polypeptide of the present invention to be used for, as affinity chromatography or immunoprecipitation with the standard technique isolated polypeptide.In addition, this antibody can be used for surveying protein (as at cell pyrolysis liquid or cell conditioned medium in night) to estimate the degree of enriching and the pattern of expression of polypeptides.Also can be with antibody as the part of Clinical Laboratory program and diagnostic ground is used for monitoring the protein level of cell or tissue, to determine as given therapeutics or the efficient in the aspergillosis diagnosis.

Can promote to survey by making antibody and detectable material coupling.Detectable examples of substances comprises plurality of enzymes, prothetic group, fluorescent material, luminescent material, bioluminescent material and radio active material.The example of suitable enzyme comprises horseradish peroxidase, alkaline phosphatase, beta-galactosidase enzymes or acetylcholinesterase; The example of suitable fluorescent material comprises Umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichloro three azine amine (dichlorotriazinylamine) fluoresceins, dansyl chloride or phycoerythrin; The example of luminescent material comprises luminol,3-aminophthalic acid cyclic hydrazide; The example of bioluminescent material comprises luciferase, luciferin and aequorin; The example of suitable radio active material comprises ¹²⁵I, ¹³¹I, ³⁵S or ³H.

The preferred epitope that is comprised by antigenic peptide is positioned at proteinic surf zone, as hydrophilic region.The proteinic hydrophobicity profile of the present invention can be used for identifying hydrophilic region.The proteinic antigenic peptide of the present invention comprises the individual successive amino-acid residue of at least 7 (being preferably 10,15,20 or 30) in the aminoacid sequence of the sequence that is selected from SEQ ID NO:115～SEQ IDNO:171, and comprise proteinic epitope, thereby antibody and this protein of anti-this peptide form specific immunocomplex.

The preferred epitope that is comprised by antigenic peptide is positioned at the zone of proteinic surface protein enzyme, as hydrophilic region, hydrophobic region, α zone, β zone, spiral (coil) zone, corner (turn) zone and movable (flexible) zone.

Immunoassay

To carrying out according to the qualitative of polypeptide of the present invention or quantitatively definite available any method well known in the art in the biological sample.Specific polypeptide level provides special advantage in the biological sample for measuring based on antigenic technology.

Wherein, specific recognition is provided by primary antibody (polyclone or monoclonal), but secondary detection system can be utilized fluorescence, enzyme or other secondary antibodies of puting together.As a result, obtained immunocomplex.

Therefore, the invention provides the method whether certain organism of diagnosis has infected aspergillosis, the method includes the steps of:

● the separating bio product that imitate from this doubtful organism that infects aspergillosis,

● this biological sample and antibody according to the present invention are reacted,

● determine whether to form immunocomplex.

Also available urea and neutral detergent extract tissue is used for western blotting or spot/narrow line mensuration with release protein.This technology also can be applicable to body fluid.

Other methods of expressing based on the detection proteinase gene of antibody comprise immunoassay, as enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA).For example, can will be used as the probe of immunosorbent and enzyme labelling to the special monoclonal antibody of proteolytic enzyme protease protein matter is surveyed with quantitative.The amount that is present in the amount useable linear regression Calculation machine algorithm reference standard preparation of the protease protein matter in the sample calculates.In another ELISA measures, two different monoclonal antibody specifics can be used for surveying protease protein matter at biological fluid.In this was measured, one of antibody was used as immunosorbent and another probe as enzyme labelling.

Above-mentioned technology can be used as " step " or " two steps " mensuration substantially and carries out." one step " measures and comprises protease protein matter is contacted with fixed antibody, and without washing, mixture is contacted with the antibody of mark." two steps " comprises washing before being determined at and making mixture and the antibody of mark contacts.Other ordinary method also can suitably adopt.Usually a kind of composition that will measure system is fixed on the support, makes other compositions of system to contact with this composition whereby, and is easy to remove from sample.

Suitable enzyme labelling comprises as those from the oxydase group, its by with the generation of substrate reactions catalyzing hydrogen peroxide.The concentration of the hydrogen peroxide that the activity of oxydase mark can form by antibody/substrate reactions of measuring by enzyme labelling is measured.

Outside dezymotizing, other suitable marks comprise radio isotope, as iodine ( ¹²⁵I, ¹²¹I), carbon ( ¹⁴C), sulphur ( ³⁵S), tritium ( ³H), indium ( ¹¹²In) and technetium ( ^99mTc), and fluorescent mark, as fluorescein and rhodamine, and vitamin H.

For example, the check compound combines with the specificity of protease polypeptide and can survey the surface in a hole of the polystyrene microtiter plates in this matrix such as 96-hole by reversibly or irreversibly protease polypeptide being fixed on the matrix external.Immobilized polypeptide or other micromolecular methods are well-known in the art.For example, can be by adding the polypeptide (usually) in solution and cover microtiter plate with protease polypeptide with the concentration of 0.05～1mg/ml, in 1-100 μ l volume to each hole, and in room temperature to 37 ℃ with dull and stereotyped incubation 0.1～36 hour.The polypeptide that is not attached on the flat board can be by removing excessive solution from flat board vibration, then water or damping fluid washing dull and stereotyped (once or repeatedly).Usually, polypeptide is contained in water or the damping fluid.Dull and stereotyped with the damping fluid washing that lacks the bonded polypeptide then.In order to block the free protein binding site on the flat board, use the protein that has nothing to do with the bonded polypeptide to seal flat board.For example, the concentration among the 300 μ l Tris-HCl is that the bovine serum albumin (BSA) of 2mg/ml is suitable.Suitable matrix comprises that those contain the matrix of definite cross-linking chemistry material (as plastics substrate, as available from Corning Costar Corp. (Cambridge, polystyrene MA), vinylbenzene or polypropylene-base).If desired, can be with the pearl particle as matrix, as beaded agarose or beaded agarose gel.

Check compound and can survey by multiple method well known in the art according to combining of polypeptide of the present invention.For example, specific antibody can be used in the immunoassay.If desired, antibody can be carried out mark (as fluorescent substance or use radio isotope) and direct detection (referring to as West and McMahon, J.Cell Biol.74:264,1977).Perhaps, secondary antibodies can be used for surveying (as Fc traget antibody partly) in conjunction with anti--AN97 antibody.In another kind of detection method, protease polypeptide is carried out mark and survey this mark (as by using labelled protein enzyme polypeptides such as radio isotope, fluorophore, chromophoric group).In the another one method, but protease polypeptide is as producing with the fused protein of the protein of optical detection such as egfp (this protein can be surveyed under UV-light).In another method, protease polypeptide can covalently be connected to the enzyme with detectable enzymic activity or merge this enzyme such as horseradish peroxidase, alkaline phosphatase, alpha-galactosidase or glucose oxidase with it.The gene of all these enzymes of encoding is all cloned, and is that those skilled in the art can be easy to obtain and use.If desired, fused protein can comprise antigen, and this antigen can be surveyed and measure with polyclone or monoclonal antibody with ordinary method.Suitable antigen comprises enzyme (as horseradish peroxidase, alkaline phosphatase and alpha-galactosidase) and non-enzyme polypeptide (as serum protein such as BSA and sphaeroprotein and milk protein matter such as casein).

Epitope, antigen and immunogen

In yet another aspect, the invention provides peptide or polypeptide, this peptide or polypeptide comprise the part that polypeptide of the present invention has epitope.The epitope of this polypeptide portion is the immunogenicity or the antigenicity epitope of polypeptide of the present invention." immunogenicity epitope " is defined as the proteinic part that can cause antibody response when whole protein is immunogen.It is believed that these immunogenicity epitopes are limited on a few locations of molecule.On the other hand, a zone definitions on the combinative protein molecule of antibody is " an antigenicity epitope ".The number of immunogenicity epitope is usually less than the number of antigenicity epitope in the protein.Referring to as Geysen, people such as H.M., Proc.Natl.Acad.Sci.USA 81:3998-4002 (1984).

As for the selection of peptide with antigenicity epitope or polypeptide (promptly containing the combinative protein molecule of antibody zone), well-known in the art be the short relatively synthetic peptide of simulated albumin matter sequence part can cause usually with by the antiserum(antisera) of the proteins react of partial simulation.Referring to as Sutcliffe, people such as J.G., Science 219:660-666 (1984).The polypeptide that can cause proteins react serum is described as proteinic primary sequence usually, can carry out characterization by one group of simple chemical principle, and the immundominance zone (being the immunogenicity epitope) that both had been not restricted to whole protein also is not restricted to amino or C-terminal.Extremely hydrophobic have 6 or still less the peptide of residue is invalid when inducing and simulateding the antibody of protein bound usually with those; Long soluble peptide, especially those peptides that contain proline residue are effective usually.People such as Sutcliffe are on seeing.For example, according to 18 in 20 peptides of these guides design antibody of having induced with HAI protein or intact virus reaction, this peptide contains 75% 8-39 the residue that has covered influenza virus (influenza virus) hemagglutinin HAI polypeptide chain sequence; Induced precipitated phase to answer proteinic antibody from 12/12 peptide of MuLV polysaccharase with from 18/18 of rabies glycoproteins.

Therefore the peptide of the present invention and the polypeptide of the present invention that have the antigenicity epitope can be used for causing the antibody of specificity in conjunction with polypeptide of the present invention, comprise monoclonal antibody.Thereby, by merging the antibody of secretion and activated protein qualitative response usually of high per-cent part in the hybridoma that obtains with having splenocyte that peptide that antigenicity has an epitope carries out the donor of immunization.See people such as going up Sutcliffe, described at 663 pages.Have the antibody that peptide or polypeptide by the antigenicity epitope cause and can be used for surveying the protein that simulated, and can be used for following the trail of the destiny in a plurality of zones of protein precursor at the antibody of different peptides, this protein precursor has experienced the translation post-treatment.The antibody of peptide and anti-peptide can be used for multiplely qualitatively or quantitatively determining that institute's mimic protein carries out, competition assay for example, even this be because shown small peptide (9 amino acid according to appointment) also can be in immune precipitation determination combination and substitute long peptide.Referring to as Wilson, people such as I.A., Cell 37:767-778 is 777 pages (1984).The antibody of anti-peptide of the present invention also can be used for purifying institute mimic protein, for example adsorption chromatography by carrying out with method well-known in the art.

According to the design of above-mentioned guide have antigenicity epitope peptide of the present invention and polypeptide preferably contains at least 7, more preferably at least 9 and 15～about 30 amino acid whose sequences most preferably from about, this sequence is contained in the amino acid sequence of polypeptide of the present invention.Yet, comprise polypeptide of the present invention the major part aminoacid sequence, contain have an appointment 30～about 50 amino acid or any length and also be considered to have epitope peptide of the present invention and polypeptide until the polypeptide that reaches the whole aminoacid sequences that comprise polypeptide of the present invention, and also can be used for inducing antibody with institute's mimic proteins react.Preferably, select to have the aminoacid sequence of peptide of epitope to be provided at the basic solvability (promptly this sequence comprises hydrophilic relatively residue and preferably avoids highly hydrophobic sequence) in the water solvent; And the sequence that contains proline residue is preferred especially.

Peptide of the present invention and polypeptide with epitope can be produced by the ordinary method of any preparation peptide or polypeptide, and this method comprises the recombination method that utilizes nucleic acid molecule of the present invention.For example, short aminoacid sequence with epitope and long polypeptide fusion should can be served as carrier by long polypeptide, and be used to produce the antibody of anti-peptide in the immunization process in recombinant production and purge process.

Peptide with epitope also currently known methods of available chemosynthesis synthesizes.For example, Houghten has described the simple method of synthetic many peptides, as the peptide of 248 of 10-20mg 13 different residues, this peptide has been represented the segmental monamino acid variant of HAI polypeptide, can be less than in 4 week preparation and characterize (by the ELISA-type in conjunction with research).Houghten，R.A.，Proc.Natl.Acad.Sci.USA?82：5131-5135(1985)。Should " simultaneously a plurality of polypeptide synthetic (Simultaneous MultiplePeptide Synthesis (SMPS) " method be further described in people's such as Houghten (1986) the U.S. Patent No. 4,631,211.In this program, but the independent resin of the multiple peptide of solid phase synthesis be contained in the independent bag with solvent perviousness, thereby make the suitableeest use of realization of the many identical repeating step that comprises in the solid phase method.

Manual program can be carried out 500-1000 or more synthetic simultaneously.People such as Houghten are on seeing, at 5134 pages.

Having epitope peptide of the present invention and polypeptide can be used for inducing antibody according to method well-known in the art.Referring to as people such as Sutcliffe, on seeing; People such as Wilson are on seeing; Chow, people such as M., Proc.Natl.Acad.Sci.USA 82:910-914; And Bittle, people such as F.J., J.Gen.Virol.66:2347-2354 (1985).

Usually, available free peptide carries out immunization to animal; Yet tiring of anti-peptide antibody can be strengthened by making peptide and macromolecular carrier coupling, as keyhole limpet hemocyanin (KLH) or Toxoid,tetanus.For example, the peptide that contains halfcystine can use linker as suitable divinyl imide benzene-N-hydroxy-succinamide ester (MBS) and carrier coupling, and other peptide can be used more generally linking agent such as glutaraldehyde and carrier coupling.

Animal such as rabbit, rat and mouse carry out immunization with free or carrier link coupled peptide, as contain the emulsion of have an appointment 100 μ g peptides or carrier protein and freund's adjuvant by intraperitoneal and/or intradermal injection.Need carry out the reinforcement several times in for example about 2 week at interval and inject to provide the useful of anti-peptide antibody to tire, this antibody can be surveyed by measuring with the free peptide that is adsorbed onto solid surface as ELISA.Tiring of anti-peptide antibody from the animal serum of immunization can increase by the selection of antagonism peptide antibody, as by peptide is adsorbed onto on the solid support, and according to the selected antibody of method wash-out well-known in the art.

Of the present invention have the peptide that immunogenicity has an epitope and identify that according to method well known in the art this peptide is that proteinic those can cause the part of antibody response when complete protein is immunogen.For example, people such as Geysen, 1984, the program of synthesizing hundreds of peptides on solid support is fast simultaneously disclosed on seeing, this peptide has the enough purity in the enzyme-linked immunosorbent assay reaction.Synthetic peptide and antibody are not surveyed its interaction from the support removal then.In this mode, the peptide with desired protein immunogenicity epitope can be identified by those skilled in the art routinely.For example, the important epitope of immunology is located with 7 amino acid whose tolerance range by people such as Geysen in the envelope protein of foot and mouth disease virus (foot-and-mouth virus), this is that by synthetic all 208 six possible peptides overlapping group realizes that this overlapping group has covered whole 213 aminoacid sequences of the present invention.

Further, the U.S. Patent No. 5 of Geysen (1990), 194,392 have described and have surveyed or the general method of definite monomer (amino acid or other compounds) sequence, and this monomer is the homeomorphic thing with its paratope of spy (antigen binding site) complementary epitope (i.e. " mimotope ") of target antibody.More generally, the U.S. Patent No. 4,433,092 of Geysen (1989) has been described and has been surveyed or the method for definite sequence monomer, and this monomer is the topological diagram Equivalent with the ligand-binding site point complementary part of specific objective acceptor.Similarly, Houghten, R.A. wait people's (1996) U.S. Patent No. 5 about the alkylating oligo peptide of mistake, 480,971 disclose group and the library that linear C1-C7-alkyl is crossed alkylating oligopeptides and this peptide, and utilize this oligopeptides group and library to determine that preferred and target recipient molecule bonded crosses the method for the sequence of alkylating oligopeptides.Thereby the present invention has the conventional preparation of also available these methods of non-peptide analogs of the peptide of epitope.

The removal of protease activity or minimizing

The present invention also relates to produce the method for the mutant cell of parental cell, this method comprises destroys or nucleotide sequence or its control sequence of disappearance proteins encoded enzyme the proteolytic enzyme that the production that causes like this is few than parental cell.

Having modification and the inactivation that the structure of strain of the protease activity of minimizing can be routinely expresses necessary nucleotide sequence by the pair cell endoprotease activity realizes.Modify or the nucleotide sequence of inactivation to can be as coding be the crucial proteolytic enzyme or the nucleotide sequence of its part for the display protein enzymic activity, perhaps nucleotide sequence for having regulatory function, it is that proteolytic enzyme is expressed necessary from the encoding sequence of nucleotide sequence.The example of a kind of like this adjusting or control sequence can be promoter sequence or its funtion part, promptly is enough to influence the part that proteolytic enzyme is expressed.Other control sequences that may modify are including, but not limited to leader sequence, polyadenylation sequence, propeptide sequence, signal sequence and termination site.

The modification of nucleotide sequence or inactivation can be finished by the cell that pair cell carries out mutagenesis and selects proteolytic enzyme throughput wherein to reduce or eliminate.Specific or mutagenesis at random can by as use the suitable oligonucleotide of suitable physics or chemical mutagen, use or dna sequence dna carried out the mutagenesis that PCR generates finish.In addition, mutagenesis can be carried out with any combination of these mutagenic compound.Be suitable for the physics of this purpose or the example of chemical mutagen and comprise ultraviolet ray (UV) irradiation, azanol, N-methyl-N '-nitro-N-nitrosoguanidine (MNNG), o-methyl hydroxylamine, nitrous acid, ethylmethane sulfonate (EMS), sodium bisulfite, formic acid and nucleotide analog.When using these medicament medicaments, the mutagenesis generally cell by under suitable condition will mutagenesis when selected mutagenic compound exist is carried out incubation and is carried out, and select to show reduce or do not have a cell that protease activity is expressed.

The modification of proteolytic enzyme production of the present invention or inactivation can be by introducing, substitute or remove one or more Nucleotide and carry out in the nucleotide sequence of transcribing or translate necessary regulatory element at proteins encoded enzyme or its.For example, Nucleotide can be inserted or removes, thereby cause the introducing of terminator codon, the removal of initiator codon or the change of open reading-frame (ORF).This modification or inactivation can be realized by the mutagenesis that site-directed mutagenesis or PCR generate according to method well known in the art.

Can carry out in vivo although modify in principle, promptly direct on the cell of expressing the nucleotide sequence that will modify, the face that is preferably as follows is modified external with demonstrating.

The example of being produced by the selection inactivation or the minimizing of host cell that makes things convenient for approach interrupts based on gene substitution or gene.For example, in the gene interrupt method, will be corresponding to native gene or the segmental nucleotide sequence of target gene at vitro mutagenesis to produce the nucleotide sequence of defective, then this nucleotide sequence is transformed into host cell to produce the gene of defective.By homologous recombination, the defective type nucleotide sequence has substituted native gene or gene fragment.Defective gene or gene fragment be also coded markings preferably, and this mark can be used for selecting transformant, and the gene of proteins encoded enzyme is modified or destroyed in this transformant.

Perhaps, the modification of the nucleotide sequence of code book invention proteolytic enzyme or inactivation can be used with protease-encoding sequence complementary nucleotide sequence by the antisense technology of having established and carry out.More specifically, the proteolytic enzyme production of cell can reduce or eliminate by the nucleotide sequence of introducing with the nucleic acid array complementation of proteins encoded enzyme, and this nucleotide sequence can be transcribed in cell and can be hybridized with the proteolytic enzyme mRNA that produces in the cell.The complementary antisense base sequences can with the condition of proteolytic enzyme mRNA hybridization under, the amount of the proteolytic enzyme of translation has has then reduced or eliminated.

Preferably the cell that will modify according to method of the present invention derives from microorganism, as is suitable for the fungi strain that the desired protein product is produced, and this protein pair cell is a homologous or allogenic.

The invention further relates to the mutant cell of parental cell, this cell comprises the destruction or the disappearance of the nucleotide sequence of proteins encoded enzyme or its control sequence, the proteolytic enzyme that the mutant cell production that causes like this is few than parental cell.

The proteolytic enzyme defective mutant cell of Xing Chenging is used in particular for expressing the host cell of homology and/or heterologous polypeptide like this.Therefore, the invention further relates to the method for production homology or heterologous polypeptide, this method comprises (a) and cultivate mutant cell under the condition that helps polypeptide production; (b) reclaim polypeptide.Term " heterologous polypeptide " is defined as herein for host cell and is the polypeptide of non-natural, has modified therein and changed the natural protein of native sequences or its and express by recombinant DNA technology the operation of host cell has been changed the natural protein that quantity changes under existing situation.

The method that the present invention produces the product do not contain proteolytic enzyme substantially has special importance, particularly Fungal Protein such as enzyme to the production of eucaryon polypeptide.The protease-deficient cell also can be used for being expressed in and has heterologous protein importance or that have pharmacy importance in the foodstuffs industry.

The purposes of proteolytic enzyme in commercial run

The present invention also relates to proteolytic enzyme according to the present invention in the industry of many selections and the purposes in the pharmacy procedure.Although obtained long term experience in these processes, proteolytic enzyme according to the present invention is compared with the enzyme of present use has many significant advantages.Depend on specific application, these advantages can comprise as lower production cost, higher substrate specificity, lower antigenicity, lower adverse side effect, when in suitable microorganism, producing higher output, more suitable pH and temperature range, better end product taste and food grade and suitable aspect etc. the aspect.

In the large-scale industrial application of food or fodder production, proteolytic ferment is generally used for improving following aspect, as the minimizing of the factor of protein solubility, extract output, viscosity or taste, quality, nutritive value, antigenicity or unfavorable nutrition, color or functional and processing aspect such as the raw-material filterability of protein.In these are used, the protein starting material can be the animal or plant source, and example comprises plant protein such as soy-protein, wheat gluten, rapeseed protein, pea protein, alfalfa matter, sunflower protein, broad bean shape soybean protein matter, cotton or til seed protein, corn protein, barley protein, sorghum gluten matter, potato protein, rice protein, coffee protein and be derived from the protein of animal such as milk protein matter (as casein, whey protein), egg white, fish protein, meat protein comprises gelatin, collagen protein, hematoglobin protein (as oxyphorase), hair, the feather and the flesh of fish.

According to an importance of proteolytic enzyme of the present invention is that they have covered and are ideally suited whole just when scope in the pH of multiple application and temperature.The danger that benefit from for example many large scale process 50 degrees centigrade or higher high relatively processing temperature infect with controlling microbial.Several proteolytic enzyme according to the present invention meet this needs, but they do not show thermostability extremely simultaneously, thereby they can stand the trial that extra heat treatment makes enzyme deactivation.The characteristic in back makes the proteolytic activity of the production approach noresidue that obtains end product.Similarly, many feeds and foods prods have slightly acidic pH value, thereby for their processing, acid or be preferred near the proteolytic enzyme of pH neutral.Also meet these needs according to proteolytic enzyme of the present invention.

The specificity of endo-protease defines according to the preferred cutting of the key between the amino group of the residue of the carboxyl of the amino-acid residue of position P1 and position P1 ' respectively usually.This preferred property can be mainly is condition with P1 (as residue positively charged in the trypsinase substrate), P1 ' (as by the hydrophobic residue in the thermophilic sporeformer proteolytic enzyme cutting) or P1 and P2 (as by the specificity cutting of adrenal medulla endo-protease between two positively charged residues).In some cases, farther residue can determine to cut preferred property, as P2 for the suis PEPA A.More known residues are to being cut with negatively influencing; It is well-known that to be proline(Pro) have resistance at the key of position P1 ' for the effect of many proteolytic enzyme.Most of endo-proteases preferably cut at hydrophobic environment or near electronegative residue.For example, industrial obtainable endo-protease such as Quimotrase (from ox pancreas, obtaining) or subtilisin, neutral metal proteolytic enzyme or thermophilic sporeformer proteolytic enzyme (all from the bacillus bacterial classification, obtaining) tend to preference hydrophobic amino acid as-Phe ,-Leu and-Tyr " afterwards " cutting.Other industrial obtainable endo-proteases for preferably-Arg and-Lys after the trypsin of cutting from ox pancreas, obtain) and the preferred papoid (complex mixture of plurality of enzymes comprises the proteolytic enzyme that obtains from papaya) that after-Arg, cuts.On the contrary, the peptide bond that is formed by little residue such as Ala, Gly, Ser, Thre and Ile and Pro is weak substrate (Keil, people such as B; Protein Seq Data Anal (1993) 5:401-407).This state reaches for pharmacy, food and drink, agricultural even chemical industry all has deep meaning.Proteolytic enzyme according to the present invention has been showed the preferred property of uncommon cutting.

Exopeptidase is only acting near the polypeptide chain end.Those effects at free N-end have discharged single amino acids residue (so-called aminopeptidase) or dipeptides or tripeptides (so-called dipeptidyl peptidase and three peptidyl peptases).Those effects at free C-end have discharged single residue (so-called carboxypeptidase) or dipeptides (so-called peptidyl dipeptidase).Carboxypeptidase is divided into 3 groups on the basis of its catalyst mechanism, i.e. serine-type carboxypeptidase, metallocarboxypeptidase and halfcystine type carboxypeptidase.Other exopeptidase is specific (a so-called pepx) or can cut peptide bond (so-called ω peptase) except that those α-carboxyls or alpha-amino group group for dipeptides.Pyroglutamyl peptidase (pyroglutamyl-peptidase) and acyl amino acyl group peptase (acylaminoacyl-peptidase) (, being respectively gene 18 and 45) that the example of this new ω peptase is as identifies in the present invention referring to table 1.

The general example of industrial application comprises the processing to the material of plant or animal-origin, and this application-dependent is in the use of pure endo-protease, and proteolytic enzyme expection according to the present invention therein can be brought into play high performance.These procedure of processings can be at the modification of large quantities of features of the protein component of starting material or (part) purifying.For example, but these procedure of processing specific aims make solvability, filterability, separability, proteins extraction output and the digestibility maximization of product, and toxicity, unfavorable taste and viscosity are minimized.In addition, this processing also can change (or partially purified) proteinic physicochemical characteristic of starting material or purifying.These advantages not only are being suitable for as the processing assistance application endo-protease of the present invention in industry the time, and also are suitable for when being applied in the animal-feed as the organized enzyme composition.Can be applied in the bread industry as bread additive specifically according to endo-protease of the present invention, as postponing the viscosity of the aging of bread or minimizing dough/pasta.Perhaps endo-protease can be used in beer and the wine industry to prevent or unwanted protein wadding is minimized.Perhaps, it can be used in the brewing industry so that the protein extract output optimization of the cereal of preparation wort.In addition, it also can be used as the milk peptizer with fabulous feature and is advantageously used in the diary industry, perhaps makes the quality of multiple milk composition form (texturishing), foaming or sedimentation (setting) feature optimization.Another application in diary industry is that new proteolytic enzyme is in cheese (Enzyme Modified Cheeses) (EMC) the purposes of preparation through enzyme modification.

In addition, can be with multiple proteins substrate handling to obtain the hydrolysate of medical science or non-medical applications with common and other proteolytic ferments combinations according to endo-protease according to the present invention.Endo-protease according to the present invention herein is effective surprisingly in the complete hydrolysis that obtains protein substrate, even the part of protease resistant is complete hydrolysis also, endo-protease also makes the allergenicity of final hydrolysate minimize or effectively suppress the formation of unfavorable taste surprisingly.

More specifically, endo-protease according to the present invention is characterised in that its preferred property in unusual peptide bond scinderin matter, especially as p1 amino acid residue A la, Gly, Ser and Thr, or residue Ile and Pro (Keil, people such as B when P1 or P1 ' position; ProteinSeq Data Anal (1993) 5:401-407).As a result, those have the component of the protein parent material of resistance to dissolve and hydrolysis with endo-protease according to the present invention to hydrolysis with the endo-protease of prior art the time.The non-limitative example of this protease resistant component comprises collagen protein, gelatin and the specific milk composition in the material of so-called extensin in the vegetable material and animal-origin.

Contain trypsin inhibitor in multiple feed such as the soybean.These protein suppress tryptic activity in as the GI-road of pig and poultry.This trypsin inhibition activity causes suboptimal protein digestion in these animals, thereby the refuse that causes increasing produces and poor economical effectiveness.This problem can be partly by solving the high bake soybean.Two kinds of dissimilar trypsin inhibitors in soybean, have been identified, i.e. Bowman-Birk type trypsin inhibitor and Kunitz type trypsin inhibitor.

The present invention provides now for the selectable approach by baking degraded trypsin inhibitor activity, be it provide can near the L-Cysteine HCL Anhydrous (EC 3.4.22, table 1) of the peptide bond place cutting between the leucine 176-aspartic acid 177 of the C-terminal of Kunitz type trypsin inhibitor (such as by Wilson (1988) at CRC CriticalReviews in Biotechnology 8 (3): among the 197-216 summary).This causes the inactivation of this trypsin inhibitor in the soybean.Find surprisingly to satisfy these standards better than the similar enzyme that is derived from other biological by fungi aspergillus niger excretory L-Cysteine HCL Anhydrous.

Proteolytic enzyme also is widely used in the cheese preparation field.In the production of cheese, essential is cheese milk to be solidified so that can separate cheese material such as casein from whey.Several milk Thrombin coagulases, be also referred to as setting accelerator and obtained describing, and comprise (ox) rennin, ox stomach en-, porcine pepsin and microbial enzyme such as Rhizomucor miehei proteolytic enzyme, Rhizomucor pusillus proteolytic enzyme and Cryptonectria parasitica proteolytic enzyme.Rennin can obtain from the ox stomach, but also can pass through as Kluyveromyces lactis (Kluyveromyces lactis) through microorganisms producing.All these enzymes are characterised in that the specificity that has the key of the peptide bond between residue in κ-casein 105 (phenylalanine) and the residue 106 (methionine(Met)) or close this peptide bond.This means that κ-casein is cut open at side-κ-casein and the contact that is called big peptide (glycomacropeptide) big peptide half point (GMP) of sugar that has negative charge by adopt these enzymes in the cheese preparation.When this took place, big peptide was diffused in the whey, and its deliquescent stabilization to casein micelles has been lost, and in case enough κ-caseins obtain hydrolysis, casein micelles promptly begins to solidify.Details for further milk enzymatic coagulation sees that (as D.G.Dalgleish, AdvancedDairy Chemistry volume 1 is seized Elsevier, London, 1992 by the P.F.Fox volume.

Current available setting accelerator makes it possible to obtain the cheese of suitable high yield, yet, will be appreciated that cheese owing to the large volume of producing, the output that the magnitude with 10 increases can constitute basic economical advantage.Subsequently, for setting accelerator huge demand is arranged in this area with (even faint) improved output.

Setting accelerator is characterised in that the substrate specificity that it is high, yet this specificity depends on pH and temperature.In general cheese production process, pH will only become low pH value in the 4.5-5.5 scope from initial pH 6.3, and final value depends on condition used in the cheese production process.Some setting accelerator change more responsive than other for pH.For example Rhizomucorpusillus proteolytic enzyme changes responsive more than rennin for pH.Except that pH, other parameter such as temperature and water-content also can influence the proteolytic enzyme specificity.Well-known most of setting accelerator shows the substrate specificity that changes with the pH that changes, thereby causes producing the proteolytic activity that change latter stage at cheese.Same well-known setting accelerator is variant on the proteoclastic degree of casein; Also show difference in the model peptide that they produce in the proteolysis process.These are the correlative factors in the cheese ripening process, and can influence character such as taste, local flavor and the quality of cheese.In some cases, setting accelerator has caused the formation of detrimental action such as bitter peptides or unfavorable taste.In addition, the variation in the proteolysis specificity can cause the minimizing of output.Well-known a kind of composition stomach en-is an example of proteolytic enzyme in many ox milk-coagulating enzyme preparations, and this proteolytic enzyme is compared with pure rennin and produced lower output and taste effect.In producing new improved cheese quality and taste, still need setting accelerator.This new setting accelerator causes aging relevant taste and the quality characteristics of fast development and cheese, in having provided basic economic interests.

Well-known total free aminoacids is extremely important in taste and local flavor generation.Especially amino acid leucine, phenylalanine, methionine(Met) and Xie Ansuan play an important role in general cheese taste and flavor compound.This total free aminoacids is converted to the actual generation local flavor and compound such as methanediol, dimethyl disulfide, methylpropanoic acid and the methylpropanal of taste by the microbial fermentation that adds in the cheese manufacturing processed.Exopeptidase plays an important role in the generation of total free aminoacids.Yet they are only worked as and have suitable specific endo-protease combination just effectively.Outward-and the appropriate combination of interior-peptase can be used in the cheese preparation, have new and cheese product improved taste characteristics thereby produce.

Enzyme according to the present invention can be used for the protein material in hydrolysis of animal source, as the mixture of full-fat milk, skimming milk, casein, whey protein or casein and whey protein.The mixture of this casein and whey protein can be as similarly ratio is suitable in the human milk.In addition, enzyme mixture according to the present invention can be used for the protein material in hydrolyzing plant source, as barley or other cereals that is used to make beer, soymilk, its enriched material or isolate, corn protein, its enriched material or isolate and rice protein wheat gluten, germination or that do not germinate.

In the field of large-scale industry process, some application only depend on endo-protease, and in other application, the combination of endo-protease and circumscribed proteolytic enzyme is crucial.Depend on general example that simple endo-protease is used and that can bring into play fabulous performance according to proteolytic enzyme of the present invention therein and comprise application as soybean or pea or grain protein processing, this application minimizes viscosity or makes foaminess or other physicochemical characteristic optimizations, bread additive in the bread industry minimizes the viscosity of dough/pasta, the auxiliary extraction output optimization that prevents the protein wadding or make cereal of processing in beer and the wine industry, fodder additives in the biological industry strengthens the microorganism active in intestinal absorption or the adjusting intestines, and the processing in the diary industry is assisted and made solidifying of multiple milk composition, foaming or settling characteristics optimization.In addition, the protein that is derived from milk or soybean or collagen protein is handled to produce so-called protein hydrolyzate with proteolytic enzyme.Although the main outlet of these protein hydrolyzates is baby preparation and the food that is used for the hospitalier, the product that is intended to supply the personnel such as the sportsmen of non-needs of medical treatment or carry out the people of slimming has formed a section that increases fast.In all these are used, protein hydrolyzate provides attracting advantage, as the allergenicity that reduces, the picked-up of promoted gi tract, amino acid needed less chemical depletion as glutamine and halfcystine, at last, during Long-term Storage, there is not protein precipitation in the acidic drinks.If hydrolysate with two and the mixture of tripeptides provide, be all these advantages capable of being combined so.Yet all commercial hydrolysates of buying are all produced by making up several endo-proteases at present.Back one method means proteinic non-homogeneous and incomplete degraded.Required two in order to obtain-and three peptide mixts, comprise multiple two-and the hydrolytic process of the combination of three peptidyl peptases are ideal.Unfortunately, having only minority is known from these enzymes of food grade and industrial acceptable microorganism, and it is obtainable to say nothing of industry.According to the present invention, several height useful two-be can obtain economically with three peptidyl peptases with relative pure state.Preferred those to the substrate that will cut show low optionally two-or three peptidyl peptases, only show that promptly minimum amino-acid residue cuts preferred property.Two of the naturally occurring peptide bond of the preferred high per-cent of those hydrolysis-or the combination of three peptidyl peptases.Although naturally occurring peptide bond is had high activity, to total hydrolysis of total free aminoacids by two-and the characteristic of three peptidyl peptases prevent.Same preferred those optimal activities are at pH 4～8 and show two of enough temperature stabilities-or three peptidyl peptases.Enough temperature stabilities mean and enzyme-to-substrate one are being arised from 50 degrees centigrade of heating after 1 hour, and remaining at least initial hydrolysis is active 40%, preferably at least 60%, more preferably 70～100%.

Although two-or tripeptides or two-and the method for effective production of three peptide mixts be key with availability according to enzyme of the present invention, be generally endo-protease with first enzyme of protein substrate incubation.The endo-protease that preferably has the wide spectrum endopeptidase activity is suitable for this state, as be used for subtilisin (available from the Delvolase of DSM), neutral metal proteolytic enzyme (available from the Neutrase of NOVO) or thermophilic sporeformer proteolytic enzyme (available from the Thermoase of Daiwa Kasei) near neutrallty condition, with stomach en-that is used for acidic conditions or aspergillosis stomach en-(aspergillopepsin) (as the SumizymeAP available from Shin Nikon, Japan).The purpose of first digestion is the heat sink feature of falling of improving solvability, reducing viscosity and minimizing water/protein mixture.In addition, with this pre-treatment of endonuclease enough two for forming-and the initiation site of three peptidyl peptases and quicken two whereby-or the tripeptides forming process be crucial.Selectively, the proteolytic enzyme that makes hydrolysate debitterize flavor with two-or three peptidyl peptases be included in this stage of process or thereafter.

The main purpose of follow-up hydrolysis is the allergenicity of product is minimized or to promote the gi tract picked-up.In the production of this hydrolysate, use two peptidyls-and three peptidyls-peptase have special importance, this is because it provides the effective way of producing hydrolysate.

The place one's entire reliance upon combination of one or more endo-proteases and one or more circumscribed proteolytic enzyme of other application in food and fodder industry.The combination of this endo-protease and circumscribed proteolytic enzyme generally is used for industry to improve as the taste of end product and the aspect of color.It is former because the generation of taste and color mainly depends on the existence of total free aminoacids.Total free aminoacids not only can obtain by circumscribed proteolytic enzyme such as carboxypeptidase and aminopeptidase, and can obtain by peptidyl dipeptidase.If with endo-protease or even two peptidyls-or three peptidyls-peptase combination, carboxypeptidase, aminopeptidase and peptidyl-pepx can generate a large amount of total free aminoacidss in the short period of time so.Yet, in all these processes, should avoid amino acid or even uncontrolled release of non-protein component so that adverse side effect minimize.

Although total free aminoacids can cause many sense of taste equally, these sense of taste are very basic (bitter, sweet, sour and " delicious (umami) "), and feel that the necessary amino acid concentration of these tastes is very high.Although these high threshold values are arranged, total free aminoacids can form main sensation by many local flavor mechanism and enhancement mechanism in low-down concentration range.One of these mechanism comprise total free aminoacids and draw combination in the reaction (Maillard reaction) with sugar in so-called U.S.A.Compare with total free aminoacids, utilize these U.S.As to draw product, can be than the total free aminoacids of record low several magnitude threshold value and develop and inundatory complicated local flavor and aroma system.U.S.A draws product usually in the culinary art of preparation food or feed product, form in the temperature that raises in curing and toasting.In handling, these have produced color and large quantities of fragrance simultaneously.In these reactions, amino group and reducing compound reaction are as the first step and finally cause whole group reaction approach.In food or feed, the aminocompound that comprises mainly is a total free aminoacids, and this amino acid discharges from the protein starting material with the multiple protein enzyme, and required reducing compound mainly refers to reducing sugar.This means in raw-material processing, should avoid the improper release of total free aminoacids and sugar so that unfavorable taste minimizes, and this unfavorable taste can be at subsequently heating steps as generating in jet drying and sterilization.The idea in back has been emphasized once more according to the higher degree of enzyme of the present invention and the benefit of low use cost.

Except that reaction is drawn by U.S.A, also can in envrionment temperature, carry out important chemical transformation to amino acid.The conversion of back one type is that enzyme is dependent, and quite general in the food of fermentation, as beer, cultured milk, cheese ripening and meat and grape wine ripening process.In these fermenting processs, total free aminoacids discharges from used starting material by the proteolytic enzyme that adds or by the hydrolase of proteolysis from used starting material or microorganism introduction.In the ripening stage, the metabolic activity of microorganism changes total free aminoacids in the derivative of the sensory quality with increase.For example, L-leucine, L-Isoleucine and L-Xie Ansuan cause valuable fusel such as amylalcohol and isopropylcarbinol (isobutanol) in beer fermentation.Similar cheese volatile matter such as thiomethyl alcohol and dimethyl disulfide can be traced back to the generation of methionine(Met) in the cheese and methylpropanoic acid and methylpropanal can trace back to Xie Ansuan.Last free amine group glutamatic acid can form strong taste enhancement because the degradation production of itself and RNA is the synergy of so-called 5 '-ribonucleotide.If with 5 '-ribonucleotide as the combination of the proper concn of 5 '-IMP and 5 '-GMP, the detection threshold of the delicious taste that is generated by L-glutamic acid is known to reduce by two orders of magnitude.

In order in all these processes, to obtain clear and definite and accurate taste effect, protein substrate should with interior-and the combination of circumscribed proteolytic enzyme come hydrolysis, wherein and circumscribed proteolytic enzyme at least a, in preferred-and circumscribed proteolytic enzyme both be pure, and preferred selective or preferably discharge preferred amino acids for the amino acid of particular group.Therefore, preferred proteolytic enzyme is characterised in that the highly selective for the aminoacid sequence that can be cut, and this idea makes the enzyme in the Aspergillus that is known as " maturing enzyme " have special importance.

Except that food and fodder industry, proteolytic enzyme also is widely used in chemistry, pharmacy, diagnosis and personal care (personal care) industry.

In personal care (personal care) industry, proteolytic enzyme is used to form peptide, and this peptide joins in the multiple product to improve the aspect as skin feel, gloss or protection.The new tendency that a kind of direct topical application proteolytic enzyme is arranged in addition.Use closely similar ground with the enzyme in the leather industry, the main purpose of a kind of application in back is cleaning, unhairing and softening skin.

In chemistry and pharmaceutical industry, proteolytic enzyme has developed into the valuable instrument of producing expensive batching or intermediate.In these industry, proteolytic enzyme is not only because its hydrolysis ability but also because it is from the ability of the synthetic peptide of natural or alpha-non-natural amino acid and be employed.A kind of selection in back is clearly by being confirmed based on the possibility of amino acid whose structure piece with endo-protease such as the synthetic aspartame of thermophilic sporeformer proteolytic enzyme from it.

Different with the situation of food and fodder industry, may need uncommon reaction conditions although will realize required chemical conversion, the solid of proteolytic enzyme-also think important advantage with regioselectivity.The general example of proteolytic enzyme in this industrial application comprises the use in producing multiple medicine intermediate such as Regular Insulin, microbiotic, feritin and ACE-inhibitor of endo-protease, aminopeptidase and carboxypeptidase.The summary of this application is provided in IndustrialBiotransformations, A.Liese, K.Seelbach, C.Wandrey, Wiley-VCH; ISBN 3-527-30094-5.

Consider required specificity, solid-and regioselectivity, have no side effect and, provide basic advantage according to the improved performance of proteolytic enzyme of the present invention to the tolerance of unusual reaction conditions such as high solvent strength.

From the angle of pharmacy, the reference of the effect of proteolytic enzyme by a great deal of be illustrated in Martindale " The Extra Pharmacopoeia " (PharmaceuticalPress, London, UK).In addition, the vital role of very special proteolytic enzyme is illustrated by the following fact in regulating various biological procedureses, and promptly many hormones are activation after its precursor molecule is by a kind of so very special proteolytic enzyme processing only.Inhibitor activity for this specific protease of some kind has been contained in the exploitation of various novel drugs.Therefore, new the identifying of proteolytic enzyme with the present available sequence provided herein of effective inhibitors.

Whole disclosures of the document that each is quoted herein all are hereby incorporated by.

Table 1

Embodiment

Embodiment 1

Measure proteolytic activity and specificity

The proteolytic enzyme specificity can be studied with multiple peptide substrates.The synthetic substrate is widely used in screening, survey proteolytic ferment to determine enzyme concn, research specificity and to probe into interaction with inhibitor in fermentation, in separation.Peptide P-N-methyl-p-nitroaniline is preferred for measuring protease activity, and this is because can follow this activity continuously and therefore make it possible to carry out kinetic measurement.The absorption that the cutting of peptide P-N-methyl-p-nitroaniline can be measured at 410nm when discharging the 4-N-methyl-p-nitroaniline increases and follows the tracks of.The p-Nitroaniline substrate is generally used for Serine and L-Cysteine HCL Anhydrous.In addition, used peptide thioesters and 7-amino-p-methylcoumarin peptide derivant.The peptide thioesters is highstrung substrate for Serine and metalloprotease, and it has showed high relatively turnover ratio, and this is because thioester bond is easy to cutting than amido linkage.The available sulfhydryl reagent of the cutting of thioesters is as 4,4-dithio pyrimidine (324nm) or 5, and 5-two sulphur two 2-nitrobenzoic acids (405nm) are followed.With respect to amido linkage, the turnover ratio of identical increase has been observed in the cutting of ester bond usually.The most well-known substrate of measuring the esterase activity of proteolytic enzyme is the p-nitrophenol derivative.The release of p-nitrophenol derivative can be dependent on used pH and monitors at different wavelength, and as having used the wavelength of 340nm in the neutral pH left and right sides time, and when surpassing pH 9, monitoring is carried out about 405nm.In addition, the hydrolysis of ester also can be by following with the volumetry of pH-stat equipment.Esterase activity is being carried out under the situation of quantitative measurment, can use pH susceptibility dyestuff.

Perhaps, can be with peptide attached on the fluorescence leavings group.When at suitable wavelength monitor, proteolysis is followed the increase of fluorescence.Normally used is peptidyl 2-phenyl amide and peptidyl 4-methyl-7-tonka-bean base acid amides.For example the release of 7-amino-4-methylcoumarin is measured with the excitation wavelength of 350nm and the radioactive wave progress row of 460nm.7-amino-4-methylcoumarin use advantage as leavings group for (380nm absorptions) of producing look and fluorescence (400nm excites, and 505nm launches) is arranged.When easily splitting the key both sides amino acid must all be arranged, the group of introducing quench fluorescence is useful.The general feature of this substrate is that peptide sequence separates the fluorescence donor groups with acceptor groups, and this receptor group serves as the Quenching of fluorescence agent.Cutting to the peptide bond between quencher group and the fluorophore will cause the essence of fluorescence to increase.Reported that several D-As are right, comprise that anthranilic acid (Abz) is as donor and 2,4-dinitrophenyl (Dnp) is as acceptor, 5-[(2 ' amino-ethyl)-amino]-naphthene sulfonic acid (EDANS) is as donor and 4-[[4 '-(dimethylamino) phenyl] azo]-phenylformic acid (DABCYL) is as acceptor.D-A is right very easily in the Abz/EDDnp representative, and this is that fluorescence has increased with 7～100 the factor, and the absorption spectrum of EDDnp does not change with pH because after complete hydrolysis.In addition, this peptide sequence can contain and reaches 10 residues and do not lose quenching effect.Along with the size increase of connection peptides, the position of the key that easily splits cannot not become specially.Therefore, except that whether definite hydrolysis takes place, also be essential to the additional analysis of product.This can be by analyzing with HPLC and separating the peptide of being produced and determine that segmental aminoacid sequence carries out.In addition, the peptide of digestion is formed and can directly be analyzed by the HPLC/ analytical technique of mass spectrum of combination.

Have the peptide of definite sequence except using, the synthetic peptide library also can be used for studying the specificity of proteolytic enzyme.Peptide is with at random or semirandom mode solid phase synthesis.Reported the preparation of a protease substrate family as people such as Meldal (PNAS USA 91,3314,1994), this preparation starts from H-Lys (Abz)-resin, with peptide resin is extended to 6 amino acid, and makes Tyr (NO2) and this peptide coupling at last.Each resin bead all has unique sequence, and after with protease treatment, the fluorescing owing to discharging the peptide that contains Tyr (NO2) of susceptible.Sequential analysis to the peptide of susceptible will provide proteolytic enzyme specific information.

Protease activity is usually with unit representation.Usually SI units (IU) are defined as that per minute shifts the required enzyme amount of 1 micromole's substrate under the condition of determining.For proteolytic enzyme, IU can relate to the hydrolysis of 1 micromole's peptide bond in the per minute.Yet under the situation of proteolytic enzyme unit, departing from international definition is rule rather than exception.In the pattern peptide of a key of specificity cutting, the calculating of IU is very simple, for can be in a plurality of positions on multiple degree the proteolytic enzyme of scinderin matter substrate, use many unit definition that depart from.Except that used unit definition, any hydrolysising experiment all needs to describe enough conditions that unit is measured of carrying out.This condition comprises as concentration of substrate, enzyme-substrate ratio, pH and temperature.The general mensuration of determining the proteolytic enzyme given activity comprises protein substrate, as oxyphorase, Regular Insulin or the casein of sex change.Under the fixed condition, digest polypeptide in the interval with proteolytic enzyme at a fixed time.Indigested and big polypeptide is precipitated with TCA, and the TCA soluble product is by measuring 220 or the absorption of 280nm or by with Folin reagent (folin reagent), triketohydrindene hydrate, fluoro-2,4-dinitrobenzene/dansyl chloride, titration soluble peptide or TNBS method or fluorescence are usually determined.After hydrolysis the marked product, also can use the peptide substrate that carries out mark with specific dyestuff or fluorophore such as fluorescein.In addition, the lab analysis instrument of available standards adopts the standard amino acid analytical procedure.For understanding the size distribution of the peptide that generates by proteolytic enzyme, can carry out the gel chromatography experiment.In addition, the better resolving power of the model peptide that is generated by proteolytic enzyme can be by adopting inverse technique HPLC to obtain.The degree of protein substrate hydrolysis is typically expressed as degree of hydrolysis or DH.Under the stable situation of hydrolytic process pH, DH can derive from the alkali consumption (enzymically hydrolyse of food protein (Enzymatic Hydrolysis of FoodProtein) in the hydrolysis, J.Adler-Nissen, 1986, Elsevier Applied SciencePublishers LTD.).DH relates to the multiple useful functional property of hydrolysate, as solvability, emulsifying capacity, foaming and froth stability, vibration expansion, organoleptic quality.In addition, taste is an important aspect of hydrolysate food grade.Bitter taste is a subject matter of protein hydrolyzate.The termination of hydrolysis reaction can wait and carry out by change pH, heat inactivation, denaturing agent such as SDS, acetonitrile.

Standard program according to this area is expressed the polypeptide shown in the table 1, and has carried out the purifying to small part.They are to analyze and find to have activity listed in the table 1 according at least a aforesaid method.

Embodiment 2

Directly determining of the Kcat/Km ratio of protease substrate

The synthetic substrate can be used for monitoring enzymic activity, be used for determining enzyme concn, be used for determining to suppress constant or be used to study substrate specificity in purge process.The Kcat/Km determination of ratio has provided the measurement to substrate specificity.It makes it possible to contrast the hydrolysis rate of enzyme of the same race to the different enzymes of the specificity of different substrates or cutting same substrate.This ratio has second order rate constant unit, so be expressed as 1/ (concentration. the time).The substrate that will have the Kcat/Km ratio of 10.5-10.6M-1.sec-1 scope is considered to extraordinary substrate, promptly has good avidity and fast turnover ratio.Yet some have the Kcat/Km value is very specific at the substrate of 10.4M-1.sec-1 scope.

The Kcat/Km ratio can be calculated after determining independent parameter.In this case, Km and Vm can obtain from multiple linear graph (as Hanes or Cornish-Bowden method) neutralization or by non-linear regression method.Known Vm=kcat.Et (wherein Et is final organized enzyme concentration), kcat=Vm/Et so.When product or substrate inhibition generation, perhaps when substrate precipitates with high concentration, promptly can not carry out the Kcat/Km determination of ratio by preceding method.Yet may be able to obtain the exact value of the Kcat/Km ratio of effect first-order condition under, this condition is for when concentration of substrate during well below the Km of estimation.Under these conditions, Michaelis-Menten equation: v=(Vm.S)/(Km+S) become:

V=(Vm.S)/Km, because S＜＜Km

Or v=(Vm/Km) .S=kobs.S=-dS/dt

Its quadrature is divided into lnS=-kobs.t+lnSo, and wherein So is an initial substrate concentrations, and the concentration of substrate of S when being preset time.This speed and concentration of substrate are proportional.In other words, to observe with kobs be the single order process of single order rate constant to substrate hydrolysis.Because Vm=kcat.Et, so kobs=Vm/Km=(kcat.Et)/Km.

The successive of substrate hydrolysis record is made it possible to determine kobs with graphic representation at lnS in to the figure of time.When if organized enzyme concentration is known, the Kcat/Km ratio can be inferred from kobs simply:

Kcat/Km＝kobs/Et

Measuring method: utilize initial substrate concentrations well below the Km that estimates and low enzyme concn so that can write down the hydrolysis of substrate.You will obtain the first order curve that product generates: after the substrate complete hydrolysis, the absorption of product (or flat fluorescent) will make it possible to accurately determine So, and this is because Pt=So.Kobs maybe can select match software from lnS for use to the slope of the figure of time, and (Enzfitter SigmaPlot...) determines.

NB: do not forget the concentration of substrate (S=So-P) that calculates any preset time from production concentration, this is because can not provide correct kobs (dP/dt=kobs.S is integration in an identical manner) with P to temporal mapping.

Perhaps, people can measure successive t1/2 (transformation period) from producing mirage (apparition) curve, and this is because in the single order process:

So t1/2=ln2/kobs=0.693/kobs is kobs=0.693/t1/2

Utilize this method to make it possible to check that you have correct single order decay (equal value of successive t1/2).

Embodiment 3

Proteinase gene in the inactivation aspergillus

The approach that makes the most convenient of proteinase gene inactivation in the Aspergillus genome is gene substitution technique (being also referred to as " a step gene disruption ").The basis of this technology by RothsteinRJ at Meth.Enzymol.101, p202 describes in 1983.This technology is substantially based on the homologous recombination of dna fragmentation that transforms and fungal cell's genomic dna.By double exchange, the gene of inactivation is the dna fragmentation displacement that (partly) transformed transformant.The dna fragmentation of this conversion preferably contains the selected marker to aspergillus niger.Substantially use general Protocols in Molecular Biology to carry out to the operation of DNA and the generation of inactivation construct.At first, isolation of genomic DNA from the aspergillus niger strain, this DNA is used for the inactivation to proteinase gene subsequently.The genomic dna of aspergillus niger can separate with the method for any description, and as by by people such as Graaff (1988) Curr.Genet.13,315-321 is described and well known to a person skilled in the art method.As template, this template is used for polymerase chain reaction (PCR with this genomic dna; People such as Sambrook, (1989) molecular cloning: laboratory manual (Molecular Cloning:A Laboratory Manual), 2 ^NdEdition, Cold Spring Harbor Laboratory Press, New York) to the amplification in proteinase gene flank zone.This flank zone refers to want the non-coding region of the proteinase gene upstream and downstream of inactivation herein.Each flank zone length preferably surpasses 1.0kb.

The DNA oligonucleotide of two strands is used to cause pcr amplification to each flank zone.For 5 '-flank zone, the upstream dna sequence dna homology of a primer and this proteinase gene encoding sequence starting point.This homology zone is preferably placed at the translation initiation site upstream and surpasses the 1.0kb place.Second primer be positioned at complementation and the reverse DNA sequence homology of proteinase gene encoding sequence in abutting connection with the upstream.

For 3 '-flank zone, primer be positioned at the dna sequence dna homology of this proteinase gene encoding sequence in abutting connection with the downstream.Second primer be preferably placed at complementation and the reverse DNA sequence homology that proteinase gene encoding sequence downstream surpasses the 1.0kb place.

Be included in all primers and with aspergillus niger genome homologous dna sequence dna length should minimum be 15 Nucleotide, preferred length is 18 Nucleotide.Most convenient ground, all primers should contain the recognition site of suitable restriction enzyme with aspergillus niger genome homologous sequence upstream.These extra recognition sites have promoted clone's process.

Primer and aspergillus niger genomic dna well known to a person skilled in the art that being used for PCR under the condition reacts.The annealing temperature of primer can be from calculating with the part of aspergillus niger genome homologous dna sequence dna.The fragment that will contain 5 '-flank zone and 3 '-flank zone is cloned in the carrier that available general Protocols in Molecular Biology breeds in intestinal bacteria.The gene clone that will can be used as selected marker in the aspergillus niger then is between two flank zones.Most convenient ground under the control of marker gene expression promoter in realizing aspergillus niger, is preferably endogenous Aspergillus niger promoter.The direction of insertion of the marker gene preferably direction with initial proteinase gene is identical.Final inactivation fragment contains 5 '-flank zone, preferred marker gene and the 3 '-flank zone under aspergillus niger endogenesis promoter control, and all are all in this direction and orientation.The dna clone of final construct is gone in the carrier that can breed in intestinal bacteria.

The inactivation construct is digested to remove the escherichia coli vector sequence with suitable restriction enzyme, and the inactivation fragment is separated (people such as Sambrook with standard technique, (1989) molecular cloning: laboratory manual (Molecular Cloning:A LaboratoryManual), 2 ^NdEdition, Cold Spring Harbor Laboratory Press, New York).At last aspergillus niger is transformed with the fragment of inactivation with the method that is described in the document, as use, the method that 293-299 describes by people (1991) Curr.Genet.20 such as Kusters-van Someren.Cell transformed is selected by transformation mixture is coated on the agar plate, and this flat board has selectivity for the growth of the aspergillus niger strain of presentation markup gene.After the Aspergillus strain that transforms being carried out purifying by replica plating, with southern blotting technique the representative number of bacterial strain is analyzed (people such as Sambrook with standard method, (1989) molecular cloning: laboratory manual (Molecular Cloning:ALaboratory Manual), 2 ^NdEdition, Cold Spring HarborLaboratory Press, New York).Therefore, the mycelial genomic dna of conversion bacterial strain separates with suitable restriction enzyme and digests.Restriction fragment separates with sepharose, and trace is also surveyed with the marker gene fragment of mark on nitrocellulose filter.Hybridization and washing are under the stringency condition.The bacterial strain of restriction fragment that contains the mark of correct length is thought correct.Utilize this method can select to have the Aspergillus niger strain of inactivating protein enzyme gene.

Embodiment 4

With ion exchange chromatography protein isolate enzyme

As the proteolytic enzyme by nucleotide sequence coded a small amount of that provides herein is to contain the expression plasmid of associated dna sequence, transform aspergillus niger strain and this aspergillus niger strain is grown on the suitable substratum with this plasmid and obtain by structure.After the liquid nutrient medium of having collected the cell that does not contain pollution, can be purified to the desirable proteins enzyme.

For with pure substantially isolated in form by the nucleotide sequence coded proteolytic enzyme that is provided, can defer to several strategies.All these strategies all are described in the relevant scientific literature fully (referring to as protein purification handbook (Protein PurificationHandbook), 18-1131-29 Edition AA is as being published by Amersham PharmaciaBiotech, Uppsala, Sweden).Program applicable to purifying protein enzyme from the mixture of complexity provides hereinafter.Existing selectively suitably for required enzyme feature, mensuration is necessary.For proteolytic enzyme, generally produce the synthetic peptide substrates of look as use as described in the embodiment 1.This peptide substrates is optionally for endo-protease, carboxypeptidase, aminopeptidase or ω peptase.Selectivity for specific three peptidyl peptases has been described in embodiment 11.By in relevant synthetic peptide, selecting correct amino-acid residue, can select to have required specific proteolytic enzyme.

Should determine at first whether proteolytic enzyme is secreted in the substratum, depends on the expression system of described production proteolytic enzyme, this proteolytic enzyme can be secreted maybe and can remain in the cell.If proteolytic enzyme is secreted in the fermention medium, the fragment that produces cell or these cells must be removed by centrifugal or filtration, and the limpid or clarifying substratum of gained is the starting point that is further purified.At those proteolytic enzyme is not in the situation of secretor type, must ruptured cell so that can carry out the purifying of proteolytic enzyme.In this case, the most handy rubbing machine of the cellular material of collection grind, levigate with pearl, handle filtration or centrifugal then with ultrasonic degradation or with French crushing apparatus (French press) or Manton-Gaulin homogenizer.At this proteolytic enzyme is under the hydrophobic or membrane-bound situation, must filter or centrifugal before add nonionic detergent so that the proteolytic enzyme dissolving.

After clarification steps, the purification step that can use three phases is to obtain the agnoprotein enzyme of basic pure state.In this three steps all or part of, it is essential adding stain remover.

At first or acquisition phase, with the target protein enzyme separate, partial purification and concentrating.In intermediate purification phase subsequently, the great majority of large quantities of impurity are removed, and in last refining (polishing) stage, the residual impurity of the trace of a large amount of related substanceses is removed and enzyme is dissolved in the suitable damping fluid.Depend on the characteristic and the physical properties of the proteolytic enzyme that obtains, the mode of revising slightly of the enough different proteins bond materials of those skilled in the art's energy makes this three phases optimization and use these stages under the condition of some change.Yet selectivity assay determination in all cases is indispensable, and this is because it will make it possible to the proteolytic activity of purifying is further carried out the successive monitoring.The assay determination that is applicable to this purpose comprises the use of producing the look peptide substrates as previously mentioned.

Catch in the step at first of purifying, the strong ion exchange resin of anionic type is preferred for clarifying the substratum that contains enzyme with desalination.For guaranteeing combining of desirable proteins hydrolytic activity and resin, hanging down substratum and the resin of having checked 3 or 4 different pH values under the conductivity condition.In these checks, resin is used the damping fluid balance usually, and this damping fluid has pH value and the conductivity identical with containing the enzyme substratum.Then this substratum is applied in the post under the pH condition, this condition has shown the abundant combination of permission resin to proteolytic enzyme, does not promptly have required enzymic activity and can trace back in the substratum that flows through.Enzymic activity is with successive salt gradient wash-out from ion exchange resin subsequently, and this gradient originates in the resin level pad and ends to the damping fluid that has wherein added 1 mol NaCl.To contain required active elutriated fraction according to this mensuration and collect and be ready for use on extra purification step.This extra purification step depends on the purity of required enzyme in the fraction of collection: if almost pure state, extra gel-filtration step will prove that it is for enough; If not almost pure state, using hydrophobic interaction resin chromatography after the gel-filtration step so.

Hydrophobic interaction resin chromatography is that the salts contg by the component that at first will collect from ion exchange resin is increased to 4 mol NaCl and is undertaken by removing any precipitation that forms.If the limpid component of gained does not contain required activity as a result, should activity obviously be present in the precipitation so, and can reclaim with pure substantially state.If the limpid component of gained still shows required activity in mensuration as a result, so equally this liquid is applied in the phenyl dextran resin (Pharmacia), this resin is with having identical pH and conductive high-salt buffer balance.If required enzymic activity is attached on the phenyl dextran resin, then should activity carry out following salt-free washing after the wash-out with the salt gradient that reduces continuously, carry out wash-out with chaotropic agent in case of necessity.As the front, will in mensuration, show the step that the component of active gradient is collected and finally carried out gel-filtration.If required enzymic activity debond phenyl dextran resin, many pollutents will be in conjunction with on them, thereby be present in required enzymic activity in the void volume of post before being applied to gel-filtration column, only need extra ultrafiltration step to obtain the more activity of conc forms.Gel-filtration column has not only been removed the pollutent of trace, and enzyme has been brought into when using subsequently in the necessary damping fluid.

Although this method is applicable to that usually according to the separation of proteolytic enzyme of the present invention and purifying, isolation technique is described in embodiment 4 more specifically.In this embodiment, the separation of Aspergillus proteinase is described as using the fixed bacitracin, promptly a kind of known and interactional peptide antibiotic of broad variety proteolytic enzyme selectivity.

Embodiment 5

With affinity chromatography protein isolate enzyme

The another kind of method affinity chromatography of purify small quantities proteolytic enzyme.In order to obtain the proteolytic enzyme of purified form, 100 milliliters cultures are grown in shaking in the bottle of good ventilation.Centrifugal with except that after any insoluble substance, supernatant is applied in 40 milliliters of usefulness, 0.05 mol sodium acetate Ph, 5.0 equilibrated bacitracin-dextran post.Be incorporated on the post proteolytic enzyme with replenished 1MNaCl and 10% (v/v) Virahol the acetate buffer wash-out (J.Appl.Biochem., 1983pp420-428).Active ingredient is collected, distilled water is dialysed and is applied in 20 milliliters of bacitracins-dextran post, and this post still carries out balance with acetate buffer.As preamble, wash-out carries out with the acetate buffer that has replenished NaCl and Virahol.With active ingredient promptly show required active component collect, to 5 mmoles/liter acetate buffer pH5.0 dialyse and concentrate with Amicon PM-10 membrane ultrafiltration then.In order to obtain the proteolytic enzyme of basic pure state, spissated liquid is carried out chromatography on Superdex 75 posts, this post is with 0.05 mol sodium acetate pH 5.0 balances and replenished the NaCl of 0.5 mol.

With the enzyme of purifying the further experiment susceptible of proof molecular weight that carries out on the PAGE whether with the basis of sequence data on predicted consistent.Last confirmation can obtain by the-terminal amino acid analysis of carrying out part.

Embodiment 6

Character from the new L-Cysteine HCL Anhydrous of aspergillus niger

In the present embodiment, as previously described, aspergillus gene nr28 is cloned and in aspergillus niger, cross and express.The enzyme that obtains is carried out purifying and be used for destroying the trypsin inhibition activity of soybean under multiple condition according to the program of describing among the embodiment 4.Papoid and bromeline have been used as the reference material.Bromeline is available from Sigma, and papoid is available from DSM Food Specialities Business Unit Beverage Ingredients, PO Box 1,2600 MA Delft, Holland.

Trypsinase suppresses according to Kakade, M.L., and Rackis, J.J., McGhee, J.E. and Puski, G. (1974): the method for J.Cereal Chemistry 51:376-382 is measured.Substrate N-benzoyl-L-arginine-p-Nitroaniline is degraded to N-benzoyl-L-arginine and p-Nitroaniline as the measurement to tryptic activity.Trypsinase be obtain from British Drug Houses Ltd and be derived from ox pancreas, in every gram product, contain above 0.54Anson unit.

The Kunitz inhibitor of soybean also obtains from Sigma.

Measuring before trypsinase suppresses, trypsin inhibitor with the concentration of 2mg/ml and above-mentioned L-Cysteine HCL Anhydrous in pH 3 preincubation in the 50mM sodium acetate buffer.The ratio of enzyme with zymoprotein and trypsin inhibitor 1: 100 (w/w) added.Albumin is as the negative contrast of enzyme.Remaining tryptic activity is measured after 3 hours at 37 ℃ of incubations.The results are shown in the table 2.

Many kinds of L-Cysteine HCL Anhydrouss of table 2. are to the effect of the Kunitz trypsin inhibitor enzymatic inactivation of soybean.

1	2	3	4	5
					The enzyme of check	Remaining TI activity (%)	Remaining TI activity (%) after the pepsin	Remaining TI activity (%) after 75 ℃ of thermal treatments	Remaining TI activity (%) after 90 ℃ of thermal treatments
Papoid	25	55	78	95
					Bromeline	30	62	86	99
					Aspergillus niger	26	26	28	35
Albumin (contrast)	100	100	100	100

TI: trypsin inhibitor activity

In L-Cysteine HCL Anhydrous and trypsin inhibitor preincubation, when existing, stomach en-repeats experiment.The final concentration of stomach en-with 1.3mg/ml added.The results are shown in row 3.

The experiment of having carried out another series is to check thermostability.Before in the preincubation that L-Cysteine HCL Anhydrous is joined with trypsin inhibitor, with these enzymes incubation 5 minutes in 75 and 90 ℃.The results are shown in row 4 and 5.

These results confirm that significantly these new L-Cysteine HCL Anhydrouss from aspergillus niger more have activity than present available L-Cysteine HCL Anhydrous in the trypsin inhibitor inactivation in making animal-feed.

Embodiment 7

Exopeptidase promotes the maturation of cheese and the taste of cheese

Make the aminopeptidase of encoding according to aforesaid method overexpression in aspergillus niger by gene nr 20 and 54 (referring to table 1).The purifying of these enzymes carries out according to the program of describing among the embodiment 4.The activity of the enzyme sample of purifying determines that in the phosphoric acid water damping fluid (50mM) of pH7.2 the p-nitrophenyl sulfonamide derivatives (3mM) that contains many hydrophobic amino acid residues in this damping fluid is as substrate.Substrate by the conversion of aminopeptidase by monitoring because substrate conversion and have the variation of optical density(OD) to determine at 400nm, wherein use do not contain enzyme solution as a reference.Depend on used substrate, active (A) is calculated as the variation of per minute OD, and is expressed as Phe-AP, Leu-AP or Val-AP unit.With normal cheese milk Delvo-tec ^TMThe starter culture of DX 31 scopes (DSM Food Specialities Delft, Holland) is inoculated the cheese with acquisition Gouda type, and realizes with the setting accelerator (every liter of cheese milk 50IMCU) of mean dose.In addition, every kind of circumscribed proteolytic enzyme of 25Phe-unit is joined in two kinds of experiment cheese, and contrast does not contain any circumscribed proteolytic enzyme.The cheese preparation parameter that uses is consistent with the program that half hard cheese of two kinds of cheese is used.Between cheese of testing and contrast cheese, reach such degree record in the difference aspect local flavor and the fragrance generation, promptly the cheese of experiment contains its organoleptic character of great majority after 3 week, and contrast cheese has obtained similar evaluation after 6 week.The level of total free aminoacids is shown as the twice height in the cheese of testing after 3 week; It is suitable that the level of 6 week after ripening becomes again.(Milford MA USA) carries out amino acid analysis according to the Picotag method of Waters.

These data declaration products can shift to an earlier date the keeping quality of selling in 3 months and not reducing cheese.The degree of the organ sensation feature of experiment cheese and contrast difference also is to contrast the cheese part omitted and tends to the former cheese taste of bitter taste and be overcome in having the experiment cheese of aminopeptidase.Equally, the quality of also finding cheese is more lubricated on some degree.

Embodiment 8

New specificity by gene 55 encoded protein enzymes

As explained before, some protein is formed as specific amino acid or specific tertiary structure and can tolerate enzymically hydrolyse.In this case, the new specific proteolytic enzyme of available displaying improves the amount of dissolved peptide from protease-resistant protein matter significantly.

Beta-casein is to have very limited tertiary structure but protein with very high-caliber proline residue.Many proteolytic enzyme are had any problem in cutting contains the sequence of proline(Pro), thereby beta-casein produces the hydrolysate that is rich in big protease resistant peptide relatively with common protease hydrolysis.The resistance peptide of back can make hydrolysate have many unfavorable character.For example, these big peptides have relative strong effect for allergenicity and bitter taste as everyone knows.In addition, these peptides have stoped and further have been degraded to total free aminoacids, thereby in some process, the appearance of the protease resistant peptide that these are big equals production loss.The utilizability of the proteolytic enzyme of protease resistant part that therefore, can scinderin matter and use and to become important techniques and economic benefits.

Beta-casein is represented in the cow's milk a kind of of main Caseinum componemt.This protein has carried out sufficient characterization on its aminoacid sequence, and can buy with almost pure form.Similarly, beta-casein provides and be used for the fabulous check substrate studying the enzyme cleavage site and concern between the length of the multiple peptide that enzymic hydrolysis forms.

Although present embodiment proof endo-protease subtilisin has the cutting feature of wide spectrum, very special enzyme has main influence as the adding by the prolyl endo-protease of gene 55 (referring to table 1) coding for the segmental size of the beta-casein that forms.

Having minimum value from cow's milk (freeze dried, salt-free substantially powder) is that the beta-casein of 90% beta-casein obtains from Sigma.From Bacillus licheniformis (B.licheniformis) (

Every gram 560000DU) subtilisin obtains from DSM Food Specialities (Seclin, France).By the proline specific endo-protease of gene 55 coding overexpression and be used in the program of describing among the embodiment 4 and carry out purifying in aspergillus niger.

With the beta-casein powder with the concentration of 10% (w/w) with Powder is dissolved among the phosphoric acid buffer pH7.0 of 0.1 mol together with the concentration of 0.1% (w/w).In 45 ℃ of shaking baths after the incubation 24 hours, reaction by 90 ℃ with solution heating 15 minutes and stop.In half solution (1ml contains 10 milligrams of beta-caseins), add 100 microlitre proline specific proteases, and make and be reflected at 45 ℃ and proceed other 24 hours.In 90 ℃ again after the thermal shock, with Delvolase ^TMAnd Delvolase ^TMThe sample of the beta-casein material of both processing of+proline specific endo-protease is analyzed to study accurate peptide size distribution in two kinds of samples with LC/MS equipment.

LC/MS analyzes

To use and P4000 pump (Thermoquest ^TM, Breda, Holland) and link coupled use ion trap mass spectrograph (Thermoquest ^TM, Breda, Holland) HPLC be used for characterized to the enzymatic protein hydrolysis product that produces by enzyme mixture of the present invention.The peptide of these formation PEPMAP C18 300A (MIC-15-03-C18-PM, LC Packing, Amsterdam, Holland) post and Milli Q water (Millipore, Bedford, MA, the U.S.; Solution A) 0.1% uniting of formic acid gradient in separates, and carries out wash-out with 0.1% formic acid (solution B) in the acetonitrile.This gradient originated in 100% solution A and be reduced to 70% solution B in 45 minute, kept other 5 minutes in back one ratio then.Used volume injected is 50 microlitres, and flow velocity is per minute 50 microlitres, and the temperature maintenance of post is at 30 ℃.The protein concn of the sample of injection is about 50 mcg/ml.

The details of independent peptide obtain with " scanning dependency (scan dependent) " MS/MS algorithm, and this algorithm is the distinctive algorithm of ion trap mass spectrograph.Carry out varifocal (zoom) scanning analysis completely after the scanning analysis to determine the strong ionic electriferous states of great majority in complete quality of scanning scope.MS/MS analyzes the peptide sequence information that a kind of ion in back obtains part subsequently, and this information can be used for using the SEQUEST from Xcalibur Bioworks to use (Thermoquest ^TM, Breda, Holland) and carry out database search.Used database is extracted by the OWL.fasta database, and this database can obtain at NCBI (NCBI (National Centre for Biotechnology Informatics)), and it contains the target protein that carries out used application.

By with these technology as method for screening, only have the peptide of quality in about 400～2000 dalton's scopes and be considered to be fit to further analyze with the MS order-checking.

Angiotonin (M=1295.6) is used for adjusting the suitableeest sensitivity of MS pattern and the suitableeest fragmentation in the MS/MS pattern, having carried out the constant of 60 μ g/ml inculcates, the result causes the triple charged kinds of double sum main in the MS pattern, and about 35% the suitableeest collision energy in the MS/MS pattern.

In independent sample with Delvolase digestion, the LC/MS/MS Analysis and Identification cover 40 peptides of a plurality of parts of beta-casein molecule.These peptides account for 79% of total beta-casein sequence together.Peptide can be traced back to the length of the peptide in 2～23 amino-acid residue scopes in the hold-times different on the C18 post.Altogether the peptide of Fa Xianing＜15% less than 6 amino acid.Use Delvolase ^TMA large amount of identifiable peptides have also been generated with the sample of proline specific protease digestion from beta-casein.These peptides have covered total beta-casein protein sequence＞50% together.In this sample, the remarkable homogeneous of the distribution of peptide size, this be because this peptide only in the length range of 2 and 6 residues.This result be presented at contain in the hydrolysate with proline specific protease preparation big by two-, three-to the component of 6AA peptide, this has shown and has been characterised in that the uncommon specific endo-protease obvious advantageous effect of incubation altogether.It is equally clear the endo-protease that is coded in the C-terminal cutting peptide chain of proline residue according to the endo-protease of gene 55 in these experiments.

Embodiment 9

Selectivity discharges specific amino acids and forms to promote local flavor.

Total free aminoacids such as leucine and phenylalanine not only are contained in U.S.A and draw in the reaction, and in numerous food fermentation as the precursor of required fragrance.For promote in food fermentation or in the heating of food, toast or the stage of curing promotes the formation of this fragrance, advantageously add protein hydrolyzate in these products, this hydrolysate contains these specific amino acid of high-caliber relatively free form.In the present embodiment, we have described the production that selectivity is rich in the yeast extract of leucine and phenylalanine.This enrichment is by obtaining to the favourable circumscribed proteolytic enzyme of the release of similar amino acid group is made up the endo-protease that selected amino-acid residue group has a preferred property of cutting.The preferred property of endo-protease should be complementary with the preferred property of used circumscribed proteolytic enzyme.For example our definite aminopeptidase characteristics by gene 20 and 54 (referring to table 1) coding is to have the clear and definite preferred property to the release of leucine and phenylalanine residue, the preferred property of the cutting coupling of this preferred property and thermophilic sporeformer proteolytic enzyme.Carboxypeptidase by gene 23 and 24 codings has preferred property, this preferred property and the preferred property of the tryptic cutting coupling that discharges arginine and leucine residue.Carboxypeptidase by gene 5 codings is characterised in that to have quite unusual preferred property to discharging glycine, and this preferred property can make up with some endo-protease that is present in the papoid.Carboxypeptidase by gene 51 codings can be removed glutaminic acid residue, this and the glutamate specific proteolytic enzyme coupling of being encoded by gene 43.

C180 is known can be at the N-terminal side cutting peptide bonds of big hydrophobic amino acid such as Leu and Phe from the thermophilic sporeformer proteolytic enzyme of endo-protease of Daiwa Kasei KK (Osaka, Japan) (commercial can buy as Thermoase).For the amino acid that will therefore expose discharges from the peptide of new formation, we use the aminopeptidase by gene nr20 and 54 (referring to table 1) coding.These genes are according to aforesaid method overexpression in aspergillus niger, and the purifying of these enzymes carries out according to the program of describing in embodiment 4.

Do not follow for these combinations with enzyme discharge leucines as much as possible and phenylalanine to discharge unfavorable amino acid, obviously should select condition used in the enzymically hydrolyse modestly.In addition, must make endogenous (with may be non-specific) proteolytic enzyme inactivation of yeast self.After many check incubations, designed a flow process, this flow process makes these two kinds of new proteolytic enzyme so that selectivity and validity discharge leucine and phenylalanine from yeast protein surprisingly.

In order to make yeast entogenous proteolytic enzyme inactivation, yeast suspension was placed 5 minutes at 95 degrees centigrade.Then suspension is cooled fast to the temperature that needs, and pH is adjusted to 7.0 with 4N NaOH.With the equal incubation under below the condition simultaneously of yeast, thermophilic sporeformer proteolytic enzyme and a kind of aminopeptidase.After thermal shock, with the pH regulator to 7.0 of 2000 ml yeast suspension, thereafter 680 milligrams Thermoase is added, and after stirring, add the aminopeptidase of purifying.Mixture is centrifugal then in 50 degrees centigrade of incubations 3 hours in stirring.In order to stop all enzymic activitys, with the pH regulator to 4 of supernatant, and in 95 degrees centigrade the other 45 minutes thermal treatment of carrying out.After another time is centrifugal, from supernatant, obtained to be used for the sample of amino acid analysis.Sedimentary or undissolved material is by removing in centrifugal 15 minutes with 3500rpm in Hereaus Megafuge2.0R whizzer.With the supernatant taking-up and with-20 ℃ of freezing placements.

With the supernatant sample after thawing immediately the Picotag method (Milford MA, the U.S.) according to Waters carry out the analysis of aminoacids content.

In amino acid analysis, the value of Trp and Cys is omitted, and the value of Asp and Asn is added up to a value.According to the data that obtained, in the hydrolysate of gained as a result, the ratio between L-Ala and the leucine (21.3: 11.7) is 1: 0.5.The L-Ala of commodity yeast generally is shown as 1: 0.3 to leucic ratio.

In second experiment, prepared the yeast extract that is rich in free glutamic acid.In order to realize this purpose, endo-protease (coded) with the preferred property of cutting and the carboxypeptidase (coded) that can remove these L-glutamic acid that therefore expose have been used by the gene nr 51 in the table 1 by the gene nr 43 in the table 1 at the C-of glutaminic acid residue end.By the endo-protease of gene nr 43 coding and by the carboxypeptidase (referring to table 1) of gene 51 codings according to aforesaid method overexpression in aspergillus niger.The purifying of these enzymes carries out according to the program of describing in embodiment 4.

The basic role of free glutamic acid in many fragrance forming processes fully proved, and thinks MSG, and promptly sodium glutamate is single most important flavour enhancing composition.

In the present embodiment,, will add by the enzyme product of the purifying of gene 43 codings then the pH regulator to 8.0 of the yeast suspension of 200ml heat-shocked, and with mixture 50 degrees centigrade of incubations 4 hours.Then pH is reduced to 5.0, and suspension is centrifugal.In 100 milliliters of supernatant, add gene product by the purifying of gene 51.Carried out 30 minutes in 50 degrees centigrade in the successive pH regulator with the incubation of this carboxypeptidase.After the enzyme incubation being stopped, once more with material centrifugal (on seeing) and obtain sample and be used for amino acid analysis with 95 degrees centigrade thermal treatments in 5 minutes.

According to (on seeing), in the hydrolysate of gained as a result, the ratio between L-Ala and the L-glutamic acid (30.0: 48.7) is 1: 1.6 according to the amino acid no that obtains.Commodity yeast L-Ala generally is shown as 1: 1 to the ratio of L-glutamic acid.

Embodiment 10

The local flavor that is rich in specific amino acid whose yeast is estimated

In order to prove that the protein hydrolyzate that is rich in according to specific amino acids of the present invention can generate specific fragrance, many experiments have been carried out with the yeast of describing among the embodiment in front.For this reason, the major part with these hydrolysates prepares and freeze-drying.With the performance of the powder of gained and the performance (GistexLS is available from DSM Food Specialities, Delft, Holland) of the commodity yeast extract in the standardized mixture compare under several reaction conditionss as a result.This standardized mixture is made up of a kind of hydrolysate, alkali mixture and water.

The alkali mixture contains well-mixed 22 gram Maxarome PlusPowder (the special type yeast extracts with high-content natural nucleotide in mortar, can obtain from DSMFood Specialities equally), 29.2 gram glucose, 9 gram REFEL-F fat (hydrogenant soya-bean oil, acquisition is from Barentz, Hoofddorp, Dutch) and 0.2 gram lactoyl calcium stearate (calcium stearoyl lactylate) (emulsifying agent, acquisition is from Abitec, Northampton, Britain).

All standardized mixtures all contain 5 gram yeast extract powder (promptly being rich in the material of leucine or L-glutamic acid or the yeast extract of commodity), 3 gram alkali mixtures and 3 gram water.Behind thorough mixing, these three kinds of slurries are carried out different heating means, promptly in the reaction bottle, carry out 65 minutes (liquid reactions) in 90-95 degree centigrade, perhaps in vacuum drying oven, carry out drying (vacuum bakeout reaction) at 20 millibars, perhaps in the reaction bottle at opening after the moisture loss, heat 10 minutes (baking reaction) in 120 degrees centigrade in 120 degrees centigrade.

After thermal treatment, all three kinds of products all have supposition at Vandyke brown to the color of black scope almost.Under the situation of vacuum bakeout reaction, only used light upper strata.The taste of heating product is estimated to grind to form thin powder and these powder are dissolved in the concentration of 2% (w/w) by the cake with blackening to carry out in the water that contains 0.6% (w/w) NaCl.Observation to one group of taste experimental subjects is set forth in the table 3.

Table 3

	Reference	Leucine	L-glutamic acid
				Liquid	Meat soup toasts slightly	Cold tea, the slight fragrance of a flower, zymic	Fleshiness soup more, meat, zymic
Vacuum bakeout	Burnt fried potato	Astringent taste, beans, zymic	Burnt meat soup, zymic
				Baking	Adustion, meat soup, deliciousness	Less baking, the fragrance of a flower, deliciousness	Baking, more fleshiness soup is more delicious

Embodiment 11

The whey protein hydrolysate of the non-allergenicity that forms with three peptidyl peptases

Can carry out overexpression as described by the dipeptidyl peptidase of gene 19 and 55 codings and by gene 4,9,10,12,26,35,46 and the 50 three peptidyl peptases of encoding, and carry out purifying according to the method that in embodiment 4, provides.Behind purifying, the pH of the enzyme that each is independent is just when determining by any available and technician's known method with temperature stability.In addition, the specificity of each independent enzyme can be used among the embodiment 1 method that outlines and determines.Illustrate in the selectivity experiment below by the displaying of three peptidyl peptases.

Make enzyme by gene 12 coding overexpression and be used in the program of describing among the embodiment 4 and carry out purifying in the aspergillus niger host cell.Being Ala-Ala-Phe-pNA and Ala-Phe-pNA (all from Bachem, Switzerland) with the enzyme that obtains like this and different synthetic product look substrate carries out incubation pH5 and 50 degrees centigrade.Cause the remarkable increase that absorbs at 410nm with the incubation of Ala-Ala-Phe-pNA substrate, with the incubation of Ala-Phe-pNA then not.This observation proves that significantly three peptidyl peptases excise tripeptides, and does not show amino-peptidase activity, and this amino-peptidase activity can cause the unfavorable increase of total free aminoacids.

In addition, by the favourable enzyme stability feature of enzyme demonstration of gene 12 codings, as shown in experiment below.With 4 enzyme samples respectively at 0,40,50 and 60 degree centigrade pH5 incubation 1 hour.Make each enzyme sample and above-mentioned Ala-Ala-Phe-pNA substrate in citrate buffer solution, carry out incubation then, and residual activity can be determined in each independent sample by measuring the increase that absorbs at 410nm in pH 5.0 degree centigrade sample shows 100% activity, and 40 degrees centigrade sample shows 96% residual activity, and 50 degrees centigrade sample shows 92% residual activity, and 60 degrees centigrade sample shows 88% residual activity.

Have in the general method of the hydrolysate of tripeptides at high proportion a production, can with whey protein (WPC 75) with 10 gram protein/liter concentration to dissolve/be suspended in pH be in 8.5 the water medium.For the first time the endo-protease subtilisin of enzyme temperature the region between the heart and the diaphragm and wide spectrum (

Every gram 560000DU is from DSM) carry out.Be that every gram protein enzyme enriched material is that this enzyme of 0.5% after 2 hours, is heat-treated mixture so that used endo-protease inactivation whey predigestion in 60 degrees centigrade with concentration.Add then with temperature regulation to 50 degree centigrade, and with three peptidyl peptases, then whole mixture is carried out incubation up to the level that reaches the tripeptides of wanting.The further procedure of processing of the hydrolysate of Huo Deing depends on specific application like this, but can be in conjunction with microfiltration and centrifugal and follow-up evaporation and spraying drying.

Sequence table

<110>DSM?NV

＜120〉the new gene of encoding novel proteolytic enzymes

<130>20095WO

<160>171

<170>PatentIn?version3.1

<210>1

<211>2520

<212>DNA

＜213〉aspergillus niger

<400>1

cgcaggcgtc?cgttgcgccg?cgaaaacctg?ccgagtgggc?cgtttaggct?ttgggtctcc 60

ccacgatgta?agcataatca?ttctgtgcct?gagtgtgaat?tctcctgttg?gaggctgcat 120

cttaattctt?aactgcatga?aaagcacttg?ggtgctattt?tctttttcct?ttctttcttt 180

tccgtgttca?tttccattcc?cttgctcttc?ttctttgtgt?cgacatttac?aaatcacatt 240

tttcttatac?tttcttttct?tcacctcgtt?tcttcctatt?cactctctgt?gttcagcatt 300

cgttatcagc?actttatctt?ttgctcgtct?cttttatctt?cacttgtttg?tgcctttcca 360

ctagcaatct?atcgtttgat?ctttctagag?cattgtcttg?attgtgtcat?tctgtcattg 420

actccggcta?tgaaatatta?ttctcaatct?gcctaaaacc?aaattctact?ctatcactac 480

acatttgtat?cacctgatct?ggctgagata?ggagagtccg?gcatctcatc?gtctgcatca 540

gacaattgcg?ataaattcat?tgcttgcacc?tgttattgat?tcttccaagt?tatgcatctc 600

ccacagcgtc?tcgttacagc?agcgtgtctt?tgcgccagtg?ccacggcttt?catcccatac 660

accatcaaac?tcgatacgtc?ggacgacatc?tcagcccgtg?attcattagc?tcgtcgtttc 720

ctgccagtac?caaaaccaag?cgatgctcta?gcagacgatt?ccacctcatc?tgccagcgat 780

gagtccctgt?cactgaacat?caaaaggatt?cccgttcgtc?gtgacaatga?tttcaagatt 840

gtggtagcgg?aaactccctc?ttggtctaac?accgccgctc?tcgatcaaga?tggtagcgac 900

atttcataca?tctctgtcgt?caacattggg?tctgatgaga?aatctatgta?catgttgctc 960

gacacaggcg?gctctgatac?ctgggttttc?ggttccaact?gcacgtccac?accctgcacg 1020

atgcacaata?ccttcggttc?ggacgattct?tcgacccttg?aaatgacatc?ggaagagtgg 1080

agtgtgggct?atggaactgg?gtctgtcagc?ggcttgctag?gaaaagacaa?gctcacgatt 1140

gcaaatgtca?ctgtacgcat?gactttcgga?cttgcttcca?acgcatcgga?taacttcgag 1200

tcgtacccaa?tggacggcat?tctcggtctc?ggtcgaacca?acgatagttc?ctacgacaac 1260

ccaacattca?tggatgccgt?tgcagaaagt?aacgttttca?agtcgaatat?cgttggcttc 1320

gccctttcac?gtagccccgc?caaggatggc?acggtcagct?ttggcactac?tgacaaggac 1380

aagtacaccg?gcgatatcac?ctacaccgat?accgtcggat?cggacagcta?ttggcgcatt 1440

cccgtggacg?atgtctatgt?tggcggcact?tcatgcgatt?tctccaacaa?atcagccatc 1500

atcgataccg?gaacttctta?tgctatgctg?ccttcaagcg?actcgaagac?gctgcacagt 1560

ctcattcccg?gcgccaaatc?ttcggggagc?taccacatta?ttccgtgcaa?cacaactact 1620

aagctacaag?tggcattctc?tggtgtgaat?tacaccatct?cgccgaagga?ctacgtggga 1680

gcaacttcag?gttctggatg?cgtttcgaac?attatcagct?acgacttatt?tggtgatgac 1740

atctggctcc?tgggtgacac?gtttctcaaa?aatgtgtatg?ctgtgtttga?ctacgatgag 1800

ttacgggtcg?gatttgcaga?gcgttcctcg?aacaccacct?ctgcgtcgaa?ctctacgagc 1860

tctggaacaa?gcagcacctc?gggatccact?acaacgggca?gctcaacgac?tacgacgagc 1920

tctgctagct?ctagtagttc?atctgatgct?gaatcaggaa?gtagcatgac?cattcccgct 1980

cctcagtatt?tcttctctgc?tctggcgatt?gcttccttca?tgctttggct?ctagttaacc 2040

gcatcttact?cgacgcctga?acctcgggaa?acatatgcat?tatttacaca?tgctgctgat 2100

ttgtatttgc?atatattctt?cgagcctgga?cggcgtgcgg?gtcatattac?cttacattcg 2160

aagtccttct?ctaatcaatc?aacatttatt?cttactccac?cagttctggc?tcgcaattaa 2220

ccctgtctaa?gaaaaagttg?gtatagaaca?tggcatccac?tacctggaac?attcaaagaa 2280

ccttgtccgg?gatcagtgtg?tatgacttcg?ggtacgattc?tgacatgaca?gttagcgtcc 2340

atcctgagga?ttcatcctga?tctccttacc?tagtatggac?ttatcaaagt?ccttgacgct 2400

attgtaccct?cccacagcca?tcaggatatc?gaaatcggcc?aacagcgact?tcatcacatg 2460

gcgaacaccc?tcctcaccca?tgatactgag?gccccagatc?cagagccggc?cgacgaaaac 2520

<210>2

<211>5001

<212>DNA

＜213〉aspergillus niger

<400>2

taggaaaatc?agaggcgaca?atttgctccg?atactggata?agtaccatcg?gtcacgaaat 60

tcagcaccga?ctggcttatg?tcctgctccg?ataccttagc?ggacattatg?ttagtagttc 120

taagcaagag?cccttggttc?ttggtaatcg?gcggatcaaa?aacgtaaaga?agacaaacag 180

aagcgccaca?ctaggctctg?cctccctctt?acagaagatc?tgccaggtat?cgatccacaa 240

agatatacca?aggactccaa?tgcagcagat?cgcaaccgaa?agggaaggcg?agctgtcaac 300

accgccatgt?gacttcaccg?ctcaccgcct?tagctgcgat?gggcaagacc?acgaacgatc 360

tcgctcttcc?ccagggccag?actgaggccg?attatatgat?ttttttcttt?cttttccgtg 420

tgtctcgtct?gctgctatat?tcttattttt?ctgttcggta?aagatacctc?ctaagaatag 480

acacgggttg?tttttttgtg?aattatctgt?tggtgtggtg?ctctctatcg?aaccggaaac 540

ctgaactcca?ctctgaaccg?ttacagttgg?agggtaacct?tcgttcagcg?gccaactgtg 600

tgacctcaaa?attcctgaaa?catcataatc?ggcgtcaagc?agagcccatc?gtgttgtctc 660

agttactatt?gagaagcctg?atacgggcaa?acgcttctcg?atccatgtga?gtcatgcttg 720

taccagccct?gaactccatt?gaataaaaaa?aaagcaagaa?aagagattgc?ccctccgtcg 780

ttttctgtat?taaacatcca?atcctcaacc?ggcagcctta?cattggctgc?cacagatttg 840

tgcctccata?cagcatgctt?cgtggtcttc?gtgatgtcgt?attattacaa?tttgcaatcc 900

ccttgttctt?gctattggta?tttcaaacat?ggaatgggtg?cttttcccgt?ttcaattccc 960

cgcaccctga?acctcaagca?ttgctagctt?ctctaaaatg?aataaaactg?ttatacttgc 1020

ttcctaaaag?tttgcgcttc?tggcctatgg?attactaaca?gcattttaga?ttatcgctac 1080

ggggtgtgat?cacaggattt?ggttctaaat?cacatttcca?gagaccattg?agcaaaatgt 1140

catctactca?aaagagccat?ttcaagctac?tccagaagtt?caaaccggag?tactcgccta 1200

gcgagtttgc?tcagtatgag?tcggagagaa?caggcatgag?ggtagtggtc?attgaccaaa 1260

aaggacccaa?agtcacaggt?tattttgttc?tagccacaga?gattctcgat?gattcaggtg 1320

ctcctcacac?gttggagcac?ttgtgcttta?tgggctcgcg?gaactataga?tataagggct 1380

tccttgacaa?gctagcaaca?cgtgtttatt?cgagcaccaa?tgcctggacg?gccacagacc 1440

acacggccta?caccttggac?acagcaggct?gggaagggtt?cgctcaaatc?ttgcccgtgt 1500

acctagagca?tgttatagct?ccaacactga?cagatgaagg?gtgctatacc?gaagtgcatc 1560

atattgatgg?cgctggagac?gacgctggag?tcgtctactc?ggagatgcag?ggtgtgcaga 1620

ataactctgc?agagttaatc?gatctaaccg?ctcgtcgatt?gacttacccg?catggtgtag 1680

gttttcgcta?cgagacaggc?ggtatgatgg?agcagctccg?cgtcctcacc?gcggaccgta 1740

tccgagcgtt?ccatcgtgag?atgtaccagc?ccaagaactt?atgcctaatc?atcacaggcg 1800

aagtagatca?ccagaacatg?ctggagacct?tggacaagtt?cgaagatact?attctagatg 1860

tcattcccag?tcctgattca?cctttcaaga?ggccgtgggt?agattccaag?caggcgccgc 1920

cattggagaa?gtccattgtc?cagactgtgg?aatttccgga?agaagatgaa?tctttcgggg 1980

agatagaaat?tagattcctc?ggtccggact?gtaccgaccc?tgttcaaagt?gagtgttccg 2040

ctgtcctcat?ttcgaagata?tacttactct?gttatagccg?gggctgtcaa?tgttgcattg 2100

ctgtatctgg?ccggttcatc?tgcttctcta?ttggataaca?tcctggttga?gaaggagcag 2160

ctcgccagtg?ctgtctatta?tgctaccgaa?gatcatccca?gcattgagat?ccgcttcaca 2220

ttaaccagtg?tggagacaga?gaaactcgcg?aaggtagagc?aacggttttt?cgaagtgctc 2280

aaggacgcta?tggagaaaga?tttagacatg?aggtatatca?aggagtgcat?tgaccggcaa 2340

agacggacct?ggaagttctc?taccgaaagc?tccgcctctt?cctttgcgga?gtacgtgatc 2400

tcggattttc?ttttcggaaa?gagagacgga?tcgactatgc?ttgatgttgc?gaccttgcaa 2460

gagtacgacg?tgctggagaa?gtggagtgaa?gaacagtggc?gcagttttat?caaaacatgg 2520

atttctgatg?ccaaccatgt?cactatcctt?ggtgttccgt?ccgttaagat?gtctgacaca 2580

ttaaagaagg?aggaggaagc?tagagtcgca?gagcaaaaga?agcgcttggg?tgatgagggg 2640

ctgaagaagt?tggccgacaa?gctggaaaaa?gctaaagctg?aaaatgacaa?ggagatcccc 2700

aaggagatgc?tggagaggtt?ccaaatccct?ggaatagagt?ctatccattt?cgtggacact 2760

actacagcca?ggtctggtgc?agccctcgat?gccgggcgcc?catcccacaa?ggcgcaaaaa 2820

ctggtggatg?ctgatggctc?tgatctgccc?ttgttcatcc?atttcgagca?tatccccagt 2880

agcttcgtgc?agctctccct?cctcatctcg?gcacaggccg?tacctgtgca?gcttcgtcca 2940

ctgctgtctg?tgtatactga?ggcattcttc?aacctgcctg?tcaaccggaa?cggggaaacc 3000

atcaactttg?agcaggtggt?tgtcgagttg?gaaagggata?ctgttggcta?ctccatggaa 3060

ggagctagaa?gcctaggaaa?ctcggagatg?ttgcggatct?cattccaggt?ggagcttgag 3120

aagtatcaca?cggcgatcgc?atggatccag?gaactttcct?ggaactcgat?tttcgatgtc 3180

gagcgactcc?gagcgattac?cagtcgactg?ctctccgatg?tgcccgattc?caagcgtagt 3240

ggcgacgaca?tgctcgcggc?tgttcatgtg?atggtccact?atgcagcaga?gtctattgtt 3300

cgggctcgga?gcaccttggt?gaaggcgcgt?tatttgaaac?ggatcaagaa?gcaattagca 3360

gaagagccga?agtctgtcgt?tgcgcggatg?gaagaaatca?gagatgcgct?tttccgtttc 3420

gagaacatgc?gagtcttagt?tatcgctgac?ctggagaaac?ttcaaaaccc?tgtgtcagca 3480

tggaaaccat?ttgctgagcg?tttgggtgca?ggtgcccctc?tacagcctat?cacgactaga 3540

agaccgttgc?tcagtgaggc?aggccagaag?ttgggcggta?agtcgtatgt?ggttcctatg 3600

ccgacgattg?attcatcgtt?cgcatatgct?accgcacgtg?gtttggattc?ttatgatgat 3660

ccaagacttc?ctgccttaat?ggttgcaatt?gcatacatga?acgcggttga?gggtcccctc 3720

tgggttgcag?ttcgaggcaa?gggtttggca?tatggcacga?actttgccta?taacattgat 3780

accggattcg?tcaacttcga?cgtttaccgc?tcccccaacg?cccataaagc?cttcgactcc 3840

agcaagcaga?ttgttgagga?tcacctctct?ggtgcgatgc?ccttcgatcc?cttgatgctg 3900

gagggttcca?ttagcagcat?tgtggtaagc?tttgcgaatg?aacagtcgac?aattggtagc 3960

gcagcctcag?gcagtttcat?ccgacaggtg?attcggcgcc?tgcctagcga?ctacaaggag 4020

cgggtgctca?agcaggtgcg?ggctactagc?gttgatgacg?tgaaaggcgc?tctgaaggac 4080

atcattctgc?ctttgtttaa?cccgtccacg?gccaatatcg?tggttacctg?cgctacagtg 4140

cttgaggagg?tttgtcattc?catgaagaaa?ttatgatctt?cttgtgtatc?atttactaac 4200

tgtcggttta?gactatcaag?gaaggtctcc?aggcatcggg?attcacgcct?gcggtgcagc 4260

cactcaaaga?attcgaagat?gactatgggc?tgaaggtcgg?cgatgacgag?gacgaggagt 4320

ccgacgatga?cgacgatgag?tatgaaaccg?gatctgaaga?tgaagatgac?agtgatgaag 4380

acatggagga?tgacgaagat?gatgagtgat?gcaatctata?cgacaacctc?tagacatgac 4440

aagatttatc?tgagccagtt?cctggataac?acctaggtcg?aaagaccagc?tacccctggg 4500

ggcccagata?tgccgacccg?tgtgatctgt?attgttagag?atgtctccaa?ctagcagacg 4560

cgcaatgatt?tttgatgtta?atgatatatc?gtacaacata?agtggttaac?caccacaaat 4620

ctgcacctaa?atcttagttg?tgattatcgg?tacctaccaa?acccgagtaa?acgttgccag 4680

aagtttatga?aaaagctctc?gttcttctta?tttcattgaa?tgttgtaata?aaaagtgcta 4740

gcagatgctt?tcgctatcgg?gcccactact?atgattaacc?tgaagcttag?tctcttaggt 4800

gacataacct?gttctacaca?ggctgccatt?atatttgcaa?cacacagctt?ctatctctga 4860

cctttgtgag?aataatacaa?ttactagaaa?ccccaggaga?tataaataca?ctctactcct 4920

accgaactaa?cacccagatc?gacaaattat?aaaccaaacc?acgtgacaat?atgatatcta 4980

tatatgaata?tgtataaggt?a 5001

<210>3

<211>3850

<212>DNA

＜213〉aspergillus niger

<400>3

gcgcggggtt?cctgcatgtc?tttcatggtt?gggaggtata?tgtacatgta?atgccgttgg 60

gttctggata?cccacaataa?agtgtttcta?ctattacttt?tgcattgtta?gactttgaag 120

gctgctctgt?aagggtccat?tccgttggag?tcaaacaacc?gtctgtccgc?gggcatatgt 180

ctcagtctga?ggtgagaact?gcaatataca?ctccaagctt?tctcaatctg?atgcacagat 240

tgcaaacatc?tcccttgctg?tgtggtaaag?tctccctttc?tcggtgaacc?ttcttcgggg 300

tcgcaactga?ttccattgac?cctcaccatc?tgtcgaattg?ttcccgaaag?ctcgcgtcca 360

aataggcttt?acggacgcca?cagctgctcg?aggccatctc?caagggggaa?tgaacaatgg 420

aatggtaagc?cgctaagaaa?ggggaactgt?cggttctggt?cgattggcag?ggtcagggca 480

gtgcttgggc?tgttcctctg?gccctccctt?gcttcaagct?ggccccgatt?tggcagctcg 540

tgaagctcac?cagacattca?gccagccaga?tgactggcac?ctttccgctg?ggcataaatt 600

ggccctggca?gccatgtcat?gccaatcttg?gctccaggac?cagtcattcc?ttttcttgtt 660

cctgtcaaac?agatcaagtc?ctcgaggatg?ggagctcttc?agtggctgtc?catcacggct 720

gctgcggcct?ccgcagtgtc?agccttgacc?ccggagtaag?tatctccaat?catttggaac 780

tggcccatat?tgtgcatagc?taaccagctt?acctgcatag?gcagatgatc?ggtgccccac 840

ggagaaccga?agttatacca?aacccctccg?gtgtatgccc?attgccaggt?ccagccttac 900

aaagaagcgt?cgtctgctga?cacgagaagg?acaccggtct?attctcgacc?tcccaatggt 960

cgtttgacac?tcattctgag?agcacctggt?ggagcttgat?cgacctccaa?tcgggcaaga 1020

ccaccactct?caccgatgat?agcgatatcg?aggagatcat?ctggcttggc?tctgacaatt 1080

ctacgctcct?ctacatcaac?agcaccaacg?cgcaggttcc?cggtggcgtg?gagctgtgga 1140

ttgcggactc?ttctgacttt?gcaaatgcgt?tggttcaggc?ctttaacgat?gcctctgcag 1200

actagtgcta?atcctacttg?gtgcagttac?aaggcagcct?ctctctccgc?cggttttctc 1260

ggcatcaaat?caaccgtgac?agattccggc?gacgtgcatt?tcatccttcg?tggaaagtcc 1320

tatcccaacg?gaacggcata?caatgatcag?ctcgccgaga?cctatcccag?tacagcccgc 1380

atctacgaca?gcatctttgt?gcggcactgg?gacacttacc?tgaccaccgc?ctcccacgct 1440

gtattctccg?gtaccctgca?aagctcgacc?agcgacgacg?gcaatgttca?atatacctct 1500

tcagggggat?tgaccaacct?ggttaaccca?gtcaagggtg?ccgaaagccc?attccctcct 1560

tttggaggca?acgacgacta?tgacctctcg?cctgacggca?aatgggttac?cttcaagagc 1620

aaagcgccag?agctgcctct?tgctaacaac?acggctgcct?atgtctatct?cgtcccacac 1680

gacggctctg?cgactgcctt?tgccgtcaac?ggccctgata?gtcctgcaac?cccggaggga 1740

gttgaaggag?aatccaacaa?tcccgtgttc?tcccctgata?gcgacaaaat?agcgtacttc 1800

caaatggcta?ctaatacata?cgagtcggac?cgcaacgtgc?tatacgtata?ctccatcgcc 1860

gatgacacca?tcactcccct?tgcaaaggac?tgggaccgat?cgcctagctc?cgtgacatgg 1920

gtcgatggag?acaacctcgt?cgtggcaagc?caagatctag?gacgaaccag?acttttcgcc 1980

atcccaggcg?atgcagggga?cgacttcaag?cccacgaact?tcaccgacgg?cggctccgtg 2040

tcggctcaat?acgtcctatc?caactctacc?ctcctcgtca?cgtccagcgc?cttctggaca 2100

agctggagcg?tctacaccgc?cagccctgac?gagggcgtga?tcaacacact?ggcctcagcc 2160

aacgagatcg?accccgagct?tagcggcctt?agttcctccg?actttgaaga?gttctacttt 2220

gacggcaact?ggactaccgt?aagtctatcc?ctccttgccc?ccaccaccac?atcacaaaca 2280

tactaaactc?accgcagctc?caaggatgga?tcacctaccc?ccaagacttc?gactcatcca 2340

agaaataccc?cctcgccttc?ctcatccacg?gcggccccga?agacgcctgg?gcggatgaat 2400

ggaacctgaa?atggcactcc?aaggtcttcg?ccgaccaggg?atacgtcgtc?gtccagccaa 2460

accccacagg?aagcaccggg?ttcggccagc?agctcacaga?cgctatccaa?cttaactgga 2520

gtacgccatt?ccctatcccc?aaactcccct?cttaaacata?cagctaacaa?atgaaataac 2580

agccggcgcc?gcctacgacg?acctaaccaa?agcctggcaa?tacgtgcacg?atacctacga 2640

cttcatcgac?acggacaacg?gcgtcgccgc?gggtcccagc?ttcggcgcgt?tcatgatcac 2700

ctggatccag?ggcgatgact?ttggacgcaa?gttcaaggcg?ctggttagcc?atgatggtcc 2760

gttcattggc?gatgcgtggg?tcgagacgga?tgagttatgg?tttgttgagc?atgaggtgag 2820

tggaccaagc?caaacccccc?ttttctcccc?ttacaccatt?acccctatac?aaatatgatg 2880

attctgaccg?tgtatagttc?aacggcacct?tctggcaagc?gcgcgacgca?ttccacaaca 2940

cggatccatc?cggccccagc?cgcgtcctcg?catacagcac?cccccagctc?gtcatccaca 3000

gtgacaagga?ttatcgcata?cctgtggcaa?atgggattgg?actgtttaat?acgctgcagg 3060

agaggggcgt?gcccagtcgg?tttttgaatt?tcccggatga?ggatcattgg?tatgttcata 3120

cccttttctt?cccccttttt?tctcccatga?ttatgggtgt?tgtggatgct?gatgtagcta 3180

tgtgtgtgtt?tagggtcacc?gggcaagaaa?acagcctcgt?ctggtatcag?caggtgctgg 3240

gatggattaa?tcggtattct?ggggtgggag?ggtcgaatcc?tgatgcgatt?gctttggagg 3300

atacggtgaa?tccggtggtg?gatttgaatc?cttgatcatt?ccctgttgct?cgttactact 3360

agtcagttat?gatcatgttc?tggctgggtc?ccaggataga?tggacggtta?ttactggtcg 3420

ttatattctg?tttgcagtgg?ttactagttg?gtagatcagt?tacgagatga?tgtatgagac 3480

gacagaaaga?cttatgctta?tgttgggagc?tgcttcattt?gtatatcaat?ttattcttgt 3540

agtgattgat?aactaactac?tgtctgactg?tctgtcaacc?gctatactaa?cattatacca 3600

accaaccaac?caatcaaatc?aatttctggt?tctttcttct?cttctgtttc?ttcacctcgc 3660

aactagacca?attcatcaag?gacagggcag?gacaggatct?ggtatctgaa?cggaacaacc 3720

gaaggaaaaa?caaatcatac?acaagctttt?gtacaatcaa?accataagaa?tcagtacagc 3780

tttctctttc?agcctttcca?aatgagagga?aggacagaga?aaatacaaag?atgataccct 3840

agaaacgaaa 3850

<210>4

<211>3139

<212>DNA

＜213〉aspergillus niger

<400>4

attgagtagg?ctgcgctctt?tgattctttg?cgaggtacta?aacccaaaca?ggcaattgag 60

atgttcggga?gagaatatgc?ggggatgcta?tgcctgttgc?agggatgatt?atctccacac 120

tagcgcgtta?aaggtttagt?agcctgagat?ggattgccga?tctccgaccg?ggccctgcct 180

cagccccagt?acttgtggaa?cgcgtcgaag?atttacaaca?ccatgccaga?cctaactagg 240

taataaagtt?gcgatgatgg?ggactgccgc?aaggctgtca?atttgggggc?cgaactcagc 300

ctcaacctgg?aaaaattaac?tccgattgtt?ttgatttgat?catgggagat?tacagtctac 360

gtgaaatgag?cttataaaga?acgttggagt?accggctctc?aacctgtctt?gtacctgcaa 420

gctttcctca?attcccttct?atctttctta?aaatcctctc?attagacatc?atgacgaggc 480

agacttctct?cgttcccagg?ctactaacgc?tagcctcact?agctgcactt?tcacaagcag 540

agctaggcaa?gatccaatgg?aaaggatctt?gcaacttgac?cacttatccg?gcattgatct 600

gtggaacact?agacgtgcca?tacgactaca?cggagtcaaa?ttccagcaag?acactgactc 660

tcgacatcgc?caagtggcca?gcgaccaaga?aaccagtctc?ggagcccatc?atatttaact 720

ttggaggacc?tggtgtcaat?tcgttcgagg?gccttgggct?ttatggagag?gaatttcagg 780

cgtaggttgc?agttctgctt?tgataccgca?tcttatgctg?actgacattg?ttctctagta 840

ttcttggagg?tcacaatgat?ttgatagctt?ttaacaaccg?gtttgtcgct?cttctttctt 900

gagctagaaa?catctactaa?cgttgtacct?ttatatagag?gcgttggaaa?caccatcccg 960

ttctcctgct?acagcgatga?cgccacccgt?gaactcgtcg?cccttcaagc?tcctaacgac 1020

ggcagagcgt?ccagcacggc?tttgggagaa?atctgggccc?agaacgcaaa?catcgcacag 1080

gcatgctatg?ctacgaacaa?tcaaactggt?agtcttattg?gaactagctt?tgctgcaagg 1140

gacatcatgc?aggtcgctga?tgcgctcagt?ggaaaggata?gtttggtcaa?ctactggggt 1200

aaatatcacg?gctgaaccga?gtttatactt?ttgctgacaa?tctacacttt?aggattctca 1260

tacggcacta?caatcggtgc?tgttctcgca?gccatgttcc?cggatcgaat?ggggaatgtc 1320

gcgcttgacg?gagtggacaa?ccccagagag?gctctttatg?gatagtgagt?ggcccttgaa 1380

gtttgcccga?tctggtatga?ttcagacagc?taattccttc?cgaaaagcaa?cgcacaagcg 1440

gttgtggacg?tcgacaaagt?tttcgaagga?ttctgcacgg?gctgcatggc?cgcaccggac 1500

ctctgcccta?tcgccaagga?gtacaccagc?gccgccaact?tggaagccgc?aatttacctg 1560

atgctggaaa?acctcaagta?caacccgatt?gccattcccg?aaaccggtgg?aatcgtaact 1620

tggagcgacg?tcaagtcgac?catttttgag?gccatgtacc?tgccaagctc?ttggcccttg 1680

acctctgagc?ttctttacta?cgtgcaaacc?cgcaacacaa?cgatccttgg?caactctgaa 1740

gtatacgaca?ccatcaaatc?ctacggtcaa?tcggcttctt?tgacttcggc?ttccgatgag 1800

gtcggcacgg?ccattacatg?ctccgacaag?catcgatctg?ccaccattaa?agaggtcctc 1860

ccgtacgtca?aagccagaca?ggctctgacc?aagatcggaa?gtgatggctc?ggacggcgac 1920

atgagatgcg?cgcagtggaa?tccgaagatg?ttcgccaagg?agcgctactc?cggtgacttt 1980

gaagtcaaga?cagccaaccc?ggtgttgatt?ctgagcaaca?cttacgatcc?agcgactcct 2040

cttcccgcag?cgaagaacct?gacagagacc?tttgagggaa?gtgtcttgct?cgagcagaac 2100

ggatacggtg?tatgtttata?cttccccttc?tcatcatatc?aaaagtgagc?aagcagctaa 2160

cctactgatg?atagcatact?accctgtcta?tgccatctct?ttgcactgcc?aaggccgtcc 2220

gggcttactt?caccaatggc?acattgcccg?ctgacggaac?gatctgccag?gtggacgtgc 2280

ctctgttcac?gaacttgacc?tacaaggatg?tgtggccgaa?gagtttccaa?cggagcgttg 2340

agtcgaggga?tgatgcgact?atcctcaagg?ctttgatgtc?ggtccgtgat?aagatgtcgc 2400

gacgcaggat?gtggtaagcg?catgctatag?atggggatgt?tgaaaaccca?aagcgatgac 2460

tggataatgt?tcttcatata?atttattctg?tgccgattcc?ggcatacgct?cgtcatgtaa 2520

tagagtttag?ttaatgttgc?aagggattgt?atagtattta?tttgtacacc?aacagcgctt 2580

catggagacc?ggaacttccc?tgagtacaga?atttcccatc?taggctttta?tttaactttg 2640

acaatactca?gtagtttgga?tgtcgatagc?attgaggcaa?gtaccggttg?ttgttttggt 2700

agtggagtga?atcaggactg?tcccacaaca?atccgctgat?ctgaatgttt?gatgtttcga 2760

tctgaggtat?ctgagctgcg?agcctccaaa?tcggcaaccc?catacttcag?ggatattcca 2820

gaacattact?agccttggag?gctttgaggt?ttagctgcag?ggcggtcaaa?ctaattgatt 2880

tctctcaatt?gtcctaccgc?tattgagtga?tctttagtgg?atgagtggaa?cggagaattc 2940

ccggtcggat?aatgcctgca?ttagtattgg?ccaacagagt?attccatcaa?cgactcccgc 3000

gctgacattt?aatccatgca?caccctgaat?tacctcgagc?ttattctcgg?tcctacattt 3060

gatcctctca?tactttccct?tatgtccttg?cccacttgct?ttacagattg?aatccggatc 3120

cttcagcact?cggcagaca 3139

<210>5

<211>2940

<212>DNA

＜213〉aspergillus niger

<400>5

atagcagaac?agaacatgta?tcttgtcaac?gaattgattg?atttcagcac?ggaaatgttc 60

aaggcatgga?acaattgctt?ctgacccgga?accgcggatt?gcaaggatgg?atatgtggat 120

cagatggtgt?gaagaagatc?tgtctgttag?catacttcat?aagttccaag?gaggagtaaa 180

tgtggagtca?taagagtaat?atcgaatatc?atagaatggg?gttgtcttgc?taacggtgaa 240

tcggactccg?tcggctctgc?cagcgatcgg?caggtcacgc?gtctgcgtaa?cttggtactt 300

atcttcccag?tcacctaagc?actgttactc?cactcttctc?tctttgacga?taagaagata 360

gcagagactc?ttgataaaaa?gggttgggag?catgctttga?atttcttcac?tccgagagct 420

ttctgtcccc?atctggtctc?ttgttgtccc?cgatgtacta?ctctctctgg?gttgctgcct 480

tggtggccgc?gctgcccgtc?tcccgggccc?agtttgtggc?tccgcccacg?gatctcattc 540

ccaccaaggg?atatctcgac?atccccgtcc?gctacaaaca?ggtccccacc?ggcatttgtg 600

agactgatcc?cagtgtcaag?agcttctccg?gttacgtcga?tgtcgctgag?catgagcaca 660

tcttcttctg?gttcttcgag?gcgcgcaacc?aagatcccac?cgaggctccc?ttgaccgtct 720

ggatcaatgg?aggcatgtct?gaccccggtc?attatttcct?tccaattgct?aaccgttcgt 780

aggtcctggt?tcctcctcca?tgatcggctt?gttccaagag?cacggcccat?gcggcattga 840

cgccaatggc?tccgtctaca?acaaccccta?ctcctggaac?aacgccagca?acatgctcta 900

catcgaccag?cccgtgcaga?ccggcttctc?ctacagcatt?ccggttcccg?gctatgtgga 960

ttcttccaca?gacaatgtta?ttgctctgcc?ctcccccgcc?tgccccgact?atgcagcgga 1020

tatgttctgt?ggcacttact?cctaccccaa?cgtgagcctt?acggctaatt?ccaccgacaa 1080

cgccgccccc?aacttctacc?gcgccctaca?gggttttatg?ggcgcatttc?ctcagtactc 1140

gcgcgaaacc?ttccacttca?ccacggagag?ttatggcggc?cactacgggc?ccgtcttcaa 1200

cgagtacatc?gaggagcaga?acgcccatct?ccagccggga?gccaagaaga?tccaactggg 1260

cagtgtgatg?atcggcaatg?gctggtatga?cccgattatt?caataccagg?cctactacaa 1320

ctttacggta?cactacctgg?tttctgatcc?ttatccttac?agcctggaca?ctaatctatc 1380

tacaggtata?tccgggcaac?acatacgact?acctgccatt?caacaagtcc?atcagctcgc 1440

tgatgtacaa?caacctctat?ggccccggaa?actgcctcga?ccagctctac?gactgcgccg 1500

cccgaggcat?cgacgagatc?tgcagcactg?ccgacgattt?ttgcgccaac?gaggtcgaaa 1560

acgtctacga?catttactcc?ggtcgggatg?agtatgactt?tcgtgaactc?actccggacc 1620

cgttccctta?cgagttctac?gttgactacc?tgaacaaagc?gtccgtgcag?gccgccatcg 1680

gcgcatacat?caattacacg?gagagcaaca?acgctgttgg?actcgccttt?tcgtccaccg 1740

gtgacgacgg?gcgactcatg?aacaccatcc?aggatgtggg?caagctgctc?aaacagggtg 1800

tcacggtggt?catgtacgcc?ggggatgccg?actataactg?caactggctg?ggtggggaag 1860

ccgtgtcgtt?gcaggtcaag?gccgccaact?tcagtagtgc?gggttacacc?aacattgtca 1920

cctcggatgg?agtgacacac?ggccaggtgc?gccaggcggg?gcaatttgcc?tttgtgcgag 1980

tgtatgagag?tggacatgag?gttcccttct?atcaaccctt?gcttgcgctg?gagatgtttg 2040

agcgcgtcat?tggcggcaag?gatgtggcga?cgggaaagat?tcccatctcg?tcgagtttac 2100

agacggtggg?cacgcccaag?agttactacc?gggagggcaa?cagcacgatt?cagtgggagg 2160

tgttggattc?tctggcgacg?tacaacacaa?ccacgaatgc?tccgaacccg?gtgagccgga 2220

ggctgaagcg?gatgggacca?gctttgcggt?ttcagatgta?gatctgaagt?acctgcgatt 2280

gtgcattgaa?gtacattgtt?cagtgcaatt?accgatcatg?gctagctgtg?ccgcccaaag 2340

ggggaagcta?caatctaaac?gccatatctt?tcgatgcaga?caccttacga?gccatgaaat 2400

gccattttat?gatcaggcta?tctactgccg?gtgattttat?gtagtgttgg?aaattttctg 2460

ttcaatcgca?acccaaagga?accaactggc?tgtaaaagac?acaacataac?tcgccagagc 2520

caaactgtgc?gaagatatga?gaagcaaagt?ttaaacgctc?ccaagccatg?gtttccctgt 2580

caagcctcat?tcactctatt?ggcttcagct?gaaagaagag?gataaacatt?caggggtaga 2640

accagacaaa?atctggtctg?gcggggatct?ggtggggacc?cgaatccaag?ttgcgttagt 2700

ttgaggcatg?gatcgacacg?cgacgaacat?gcagtggagg?gccgctatcg?ctgttttgac 2760

tcgacatcgg?accaaaacca?aggaaattcg?tattcatcga?tcgatagttc?ttctcatctg 2820

gcaaggcatg?gatccgtctc?cccacttcag?ccggtacctc?ccggcacaac?aaaaggagca 2880

tggcagcctg?atttcctgcg?ttccaactgt?tcgtcggttg?atctccctga?tcaagtccag 2940

<210>6

<211>4550

<212>DNA

＜213〉aspergillus niger

<400>6

tgaaccatca?ggtgggacca?cagagcccac?cgtccagctc?aaggcagctc?tctattcgaa 60

gaaagaggta?ctggatatat?cgggtacttc?tagctccagg?tcgtgaagcg?ttgcgagata 120

cacaaatact?gttggcgatc?gaatgggacg?aacatgtgaa?tgttagcacc?agaagtggat 180

ggtagctacg?aacgatggga?ggcggaggtc?cgagtgtacg?atagagctgt?ggagatagat 240

tcgtatataa?tagagatata?tattcattgc?attgcattgc?tgtggtagaa?aacttgtttt 300

ggatcgcatg?aattgctggc?ctgaatgatc?aggtcagaag?acgccgcagc?tccgccggac 360

aagagccaat?cagagggcgc?caatcggggt?gtccgcccgg?ttcccttttc?agtgtctgtc 420

actgcagctc?gcctgacttc?agttcctttt?ctccttcctc?acggtcctcg?tcattcgccc 480

ctctctgttg?cagtctctct?tgttgggggg?tcttgtgaat?gtcttgcgtc?tggctccaca 540

tccacaaaag?gagcctactg?tctgtcgcta?cgaacaattc?tgttgcgagg?gccgctgcct 600

ctacctccgc?cgcgccgccg?ccgccgtcat?cgccgccgcc?tggttctaat?acttattcgc 660

ctctttatcg?ccccatcacc?aatcccatcg?gatttacttt?gtcgcctgcg?aggtcactag 720

tttctcgcaa?tcctaaattt?cctgcctatc?ggcgctctag?tcgacacttt?tctttgtgcc 780

cggccgctgc?aacgcccggt?gtcaccacga?gcatctgccc?tggtcaggcg?cccgtccgct 840

ctctcagctc?gctcattata?cactctacga?gaccccgtgc?tatacgtatc?cgtaccgacc 900

agatggattt?gaatggagac?gcaggcgcca?agcgcaagcg?cagctccatc?accacacccg 960

ccgaacggcc?cgtaaagcac?cttcgccccg?aatcgagcgc?attgacaccc?ggggattcga 1020

cgcctgccaa?tgggactgta?tacgatgtgg?aggatgatga?agatgcgagt?cgtctgctgc 1080

ctgtagggcc?tgctcaggcc?gactcaccgg?aatggcaagc?taccatagag?gaggttgtga 1140

aaagcgtagt?gtctatccac?ttctgtcaga?cctgctcctt?cgacacggag?ctgtccatga 1200

gtagtcaggc?tactgggttt?gtggtagatg?cagagaatgg?gtacatattg?acaaaccgac 1260

acgtggtttg?cccgggacct?ttctggggat?actgcatctt?tgataaccat?gaggaagtat 1320

gttgtacatt?ccacatgtgg?atcgccttat?gacagtgatg?ctgatttgaa?ttggttacta 1380

gtgcgacgtt?cgtcctgtgt?atcgggaccc?tgttcacgac?tttggaattt?tgaaattcga 1440

cccgaaggct?attcgatata?tgaaattgag?ggaactgaaa?ctgcagccgg?atgcagctaa 1500

agtgggatca?gaaattcgcg?ttgtgggtaa?tgatgcagga?gaaaaactga?gtattctgtc 1560

tggtgtcatt?agtcggctgg?atagaaacgc?gcccgaatac?ggcgatggct?acagtgactt 1620

caatacgaat?tacatccagg?ccgccgcagc?agctagcggt?ggaagttccg?gcagtcctgt 1680

agttaacatt?gatggccatg?cgattgctct?gcaggccggt?ggtcgtgcag?acggtgcagc 1740

gacggattac?ttcctccctc?tggaccgacc?gctacgcgca?ctggaatgca?tccgtcgcgg 1800

agagcctgtc?acgcgtggaa?cgattcagac?gcagtggatc?ttgaagccgt?tcgacgagtg 1860

tcgtcggttg?ggcttgacgc?ctgagtggga?ggcgaccgtg?cgtaaagcag?cgcccacgga 1920

aaccagcatg?ctggtggccg?agatcatcct?gcctgaaggc?ccggcggacg?gaaagctcga 1980

ggaaggagac?gtgctcctgc?aggtcaacgg?ggtgcttctc?acccaattca?tccggttgga 2040

tgacatcctg?gattcgagtg?ttgggcagac?agtgcgtctg?cttgtccaaa?gaggcggtca 2100

gaatgtggag?attgagtgcc?aggttggcga?cctgcatgcc?atcacgcccg?accggttcgt 2160

gacggtggct?ggaggcacgt?tccataacct?gtcttaccag?cagtcgcggc?tgtatgccat 2220

cgctactcgc?ggtgtctacg?tctgcgaggc?tgccggctcc?ttcaaactgg?aaaacacact 2280

gtcaggatgg?atcatcgact?cggtggacaa?gcggcccact?cgcaatctgg?atgagttcgt 2340

ggaggtgatg?cgaacgattc?ccgatcgttc?gcgcgtggtc?atctcgtatc?ggcatattcg 2400

cgatctccac?acccgaggca?ccagcatcgt?ctatatcgat?cgacactggc?accccaagat 2460

gcgactggct?gtgcgcaacg?acgacaccgg?tctgtgggac?ttttcggacc?tcgcggaccc 2520

tatcccagct?cttcctccgg?ttccgaggaa?agccgatttc?attcaactcg?atggtgttag 2580

ccagcctgct?gcggccgaca?ttgtgcgcag?cttcgtacga?gtatcctgta?cgatgcccct 2640

gaagctggac?ggctaccccc?aggccaagaa?gactgggttc?ggattggtcg?tcgatgcaga 2700

gaagggtttg?gtggttgtgt?cgcgagcgat?cgtgccgtac?gacctctgcg?acatcaacgt 2760

cacggtggcc?gactccatca?tcgtgaacgc?taaagtagtt?ttcctgcatc?cgctccaaaa 2820

ctacagcatc?atccagtacg?acccaagcct?ggtgcaggcg?ccggttcaga?gtgccaaact 2880

cgccaccgac?tacatcaagc?agggacagga?cacgatcttt?gtgggattca?accagaactt 2940

ccggattgtc?gtggccaaga?ccgccgtaac?cgacatcacc?actgtttcta?ttccagccaa 3000

cgcgtccgca?ccgcgctacc?gcgcgatcaa?cctggacgcc?atcactgtgg?acaccggact 3060

cagcgggcag?tgttctaacg?gtgtcctgat?tggcgaggac?ggagtggtgc?aggcattgtg 3120

gttgaactat?cttggagaac?gcacatctaa?ttcgcataag?gatgtggaat?accatctagg 3180

atttgcgact?ccatctcttc?ttcctgtcct?gtcgaaggtg?cagcagggag?agatgccgga 3240

attgcggatt?ctgaacatgg?agagctacgt?ggtccagatg?agtcaagctc?gtatcatggg 3300

cgtgtcggag?gaatggatcg?agaaggtgac?gcaagctaac?ccatcgcggc?atcagctctt 3360

catggtgcgc?aaggtcgatt?gcccaccgcc?tgggttcaac?tcagcggccg?acacgttcga 3420

ggagggtgat?atcatcctga?ccttggacgg?acagctgatc?acccgcgtct?cggagttgga 3480

tatcatgtac?gagaaggata?cgctggaagc?cctgattgtt?cgaaatggac?aagaaatgcg 3540

gatccaggtg?ccgactgttc?caacagagga?cctagagact?gaccgtgcgg?tcgtgttctg 3600

tggtgctgtg?ttgcagaaac?cacaccatgc?ggtccgtcag?cagatttcta?agctacacag 3660

cgaagtctac?gtcagcgcaa?gagtatgctc?ctcacccctt?aaccactgcc?atcaagtaac 3720

taaccaccct?atcccttcaa?cagagtcgcg?gatccccctc?ctaccaatac?ggcttggccc 3780

caaccaattt?catcaccgcc?gtaaacggcg?ttccaacccc?gaacctggac?cgcttctccg 3840

aagaagtgag?caaaatcccc?gacaacacat?atttccgcct?acgggcggtg?acattcgaca 3900

atgtgccgtg?ggtagtgacc?gtgaagaaga?acgatcatta?cgtatgccac?aacccctccc 3960

tctttacctc?caccaaattc?agagaatgtg?gagactaaca?caataatctc?cagttcccca 4020

tgtccgagta?tatcaaagac?cagtcccagc?cttccggttg?gcggaccgtg?tctcacgaca 4080

aggataaata?taaagacggc?attgcaccgg?atgctgcgaa?cttgaacccg?gatgctatgg 4140

acgaagggtt?tgatggagtc?agtgatattg?agccggattt?ggagtgattg?aaggcggact 4200

gtatagtatt?ctgtgatgag?catggctgtg?ttagatgtgt?atagcagaca?tggcttggaa 4260

tagtagaaaa?agtgaaaaat?gggaaaatga?tagagattaa?atcggttcgc?atatacgaag 4320

ttgtagatgg?atgtattgtt?tcggatgttt?acgtagtacg?tagtggctac?tctatcgagg 4380

tgtgatgata?gcattagtct?ttgatttgtt?ttcgtcccat?ctgcacatgt?actccgtata 4440

tgagaagtat?gttgctcgac?aacccaagtc?tgaaagtatg?aatgaacgaa?tcgacaacac 4500

acatgaagat?aattttatcc?aagcaaaaga?agccccattt?catcttattt 4550

<210>7

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>7

gaatgcgctg?aaaaaaatgc?tgttgatagc?ctaaggtagt?cacccgacgg?gcttgtgatt 60

ggaggaaacc?gcaatgacgc?tggtcccgtg?ccatgagctg?actcagctgg?ctgactcaac 120

gcgtgacttt?ctgcaaaggg?aaggcaccgg?catgtgattg?gtcgccttcc?gttcctgcta 180

tcgaggcgtc?atcgcctaca?gcccatcgag?tacacctcct?cttctctcct?ctctccttcc 240

ttccttcctc?tccatcctct?tctgggtttc?cattgatcac?ccagtagctg?gtattttctg 300

agctacttgc?tttttctatt?ttgatacttt?tgtgtccgta?agttcacttt?atgccctctg 360

acccccccct?tgataacccg?tgctgataag?ctccctgccc?aggtaccgta?ccttccagac 420

cgcaaggtac?ccatcctctg?cctactcatc?ccatcaccat?ctcaattcat?accggccccg 480

tagggtttca?gcaacaatga?gagtccttcc?agctgctatg?ctggttggag?cggccacggc 540

ggccgttcct?cccttccagc?aggtccttgg?aggtaacggt?gccaagcacg?gtgccgacca 600

tgcggccgag?gtccctgcgg?atcacagtgc?cgacgggttc?tccaagccgc?tgcacgcatt 660

ccaggaggaa?ctgaagtctc?tctctgacga?ggctcgtaag?ctttgggatg?aggtggccag 720

cttcttcccg?gagagcatgg?atcagaaccc?tctcttttcc?ctccccaaga?agcacaaccg 780

ccgtcccgac?tcgcactggg?accacatcgt?ccgcggctcc?gacgttcaga?gcgtctgggt 840

cactggtgag?aacggtgaga?aggagcgcga?ggtcgatggc?aagctggaag?cctatgatct 900

cagggtcaag?aagaccgatc?ctggctctct?tggcatcgac?cccggcgtga?agcagtacac 960

cggttatctc?gatgacaacg?agaatgataa?gcatttgttc?tactgtaagc?acaccttggt 1020

tcaagatcac?gctttttata?tgctctggat?atctaacgca?acttagggtt?cttcgagtct 1080

cgcaatgacc?ccgagaatga?tcccgttgtt?ctgtggctga?acggtggccc?tgggtgctct 1140

tccctcaccg?gtctcttcat?ggagcttggc?cctagcagca?tcaacaagaa?gatccagccg 1200

gtctacaatg?actacgcttg?gaactccaac?gcgtccgtga?tcttccttga?ccagcctgtc 1260

aatgtcggtt?actcctacag?taactctgct?gtcagcgaca?cggtcgctgc?tggcaaggac 1320

gtctatgcct?tgcttaccct?cttcttcaaa?caattccccg?agtatgctaa?gcaggacttc 1380

cacattgccg?gtgaatctta?tgctggtcac?tatatccccg?tcttcgcttc?ggagatcctg 1440

tctcacaaga?agcgcaacat?caacctgcag?tccgttctca?ttggcaacgg?tctcaccgac 1500

ggatacaccc?agtacgagta?ctaccgtccc?atggcctgcg?gtgacggcgg?ttacccagct 1560

gtcttggacg?agagctcctg?ccagtccatg?gacaacgctc?ttcctcgctg?ccagtctatg 1620

attgagtctt?gctacagttc?cgagagcgct?tgggtttgtg?tcccggcctc?catctactgt 1680

aacaacgccc?tccttgcccc?ttaccagcgc?actgggcaga?acgtctatga?tgtccgtggt 1740

aagtgcgagg?atagctctaa?cctttgctac?tcggctatgg?gctacgtcag?cgactacctg 1800

aacaagcccg?aagtcatcga?ggctgttggc?gctgaggtca?acggctacga?ctcgtgcaac 1860

tttgacatca?accgcaactt?cctcttccac?ggtgactgga?tgaagcccta?ccaccgcctc 1920

gttccgggac?tcctggagca?gatccctgtc?ttgatctatg?ccggtgatgc?tgatttcatt 1980

tgcaactggc?tgggcaacaa?ggcctggact?gaagccctgg?agtggcccgg?acaggctgaa 2040

tatgcctccg?ctgagctgga?ggatctggtc?attgtcgaca?atgagcacac?gggcaagaag 2100

attggccagg?ttaagtccca?tggcaacttc?accttcatgc?gtctctatgg?tggtggccac 2160

atggtcccga?tggaccagcc?cgagtcgagt?ctcgagttct?tcaaccgctg?gttgggaggt 2220

gaatggttct?aaagacgtgc?taccaccgca?tatagacttt?ctggtcattt?cggtgacact 2280

gcagatatgt?ttcttaacga?tagtttgagc?atgcttgtca?atgcccacta?gtcccgatcc 2340

ttatatgttg?catggtatct?atgagttttg?tcactatagt?gcattataca?tttgtacttc 2400

gtatgagaat?gaatcgatcg?catttacacg?catataaata?gtacccaaac?cgtctggaca 2460

tgaataaggc?ccggccagta?gtttacatac?agtgtagaaa?actaggcgta?cagacgtctc 2520

agtacgtaat?caatggttaa?aaaaaccact?cccatagaag?ccaagccata?agagcctact 2580

catgtagttc?gccactgaac?gcacccgtat?atcgtaaacc?agcagaaaga?gaaaaggaaa 2640

attgaggaaa?ggacgatttg 2660

<210>8

<211>1680

<212>DNA

＜213〉aspergillus niger

<400>8

aaaacgtgcg?cactgcaccc?actcgttccg?gctggggtct?agaaatctgg?acggtcccag 60

gcagatcggt?gcctgggcaa?aaccttgata?aaaatagctt?gttcgatctt?gagttagaca 120

gccaattgta?tactcactag?agacacttga?tgattcagtc?tgtgacgtac?gtgcacctcc 180

acactccgtc?gatggattat?gtgtccccgt?gggcacgcgg?agatcgggga?catcagtcga 240

gaaacttgcc?taatctagtg?acaagcagaa?aagtcaaagt?catccgcatg?cgacttgcag 300

atcatctaga?gggatataat?aaagtcgtgc?gtttatgacc?ttgcaggaaa?cgacccgcct 360

cctcctgcct?ctttattatc?gataccctct?gccactagcg?tcgtacatca?cacttcacaa 420

tccattctcc?tctcattcat?catgaagttc?acaaattatc?tcttgacgac?tgcaacgctc 480

gcaagcagtg?tcctagcggc?tcctgctccc?cgcaccggtt?tggaggacag?actccgtgcc 540

cggtcattgc?agcgtcaatc?acatcctctg?gcacctattc?cacttgacac?atccaccaaa 600

gagaattcca?gactcctcga?agccgacgag?aataccaccc?atgttacata?cagcagtaac 660

tgggcgggcg?cagtgcgcga?gcaaccacct?ccgcaaggca?cgtattctgc?cgtgtcggca 720

acctttcgtg?taccagaacc?cacggcgcaa?ggggggagcg?gaacgcaggc?tgggtcggcc 780

tgggtcggga?tagatggcga?cacatacagc?aacgccattc?tacagacagg?agtcgacttc 840

tacgtggaaa?acgggcagac?gtacaacgat?gcctggtatg?agtggtaccc?agactatgca 900

tatgacttcg?acctagatgt?aagcacaggg?gacacgatcg?tcgccaaggt?ggaagccatc 960

tcgccaagtc?aaggtgtagc?cactattgag?aacatatcga?cggggaagaa?ggccacgcag 1020

acgatcagag?ccccagctgc?gacagctacc?cttgccggcc?agaatgccga?ctggatcgtg 1080

gaggatttcc?agtctggcga?ctcaatggtc?gatctggctg?gctttggcga?gatcagcttc 1140

tggggcgtgc?aagcacaagg?aggagggtct?acatggggtg?tagatgatgc?gactattgtc 1200

gaactgaagc?agggcaacga?agtgttgaca?gacgtggagg?tgcaaagtga?ttcggccttt 1260

acggtgaaat?atacgagctg?atgtgatggt?atatggtcgc?atattctcat?ccttcgacta 1320

atctttctcc?ttttggacgg?tgacataatt?tttctggttg?attttgaaaa?tgccttgatg 1380

tatttcggta?gtagttttaa?ggttgatact?tccagcatgc?gggctcggct?attgggagga 1440

accaagaagg?ctatatggcc?ctcgggtatt?cgccgcattc?taaggtcgca?tgccttctcg 1500

gctttcctta?tatatatcta?ctttccattt?ccccaacctc?attcttgatg?cgagaatcta 1560

cccacacata?cagttaatca?tgtcccgtat?ataccaacta?agccgagcat?ttcagcgcag 1620

ccaagttggc?gcgctccggc?cgcgctctct?actacccgct?cggtcatccc?gctgcggctt 1680

<210>9

<211>2590

<212>DNA

＜213〉aspergillus niger

<400>9

ttacatgctc?tgtgtgttgg?taaacacgct?gtcacattct?tcatgtcctc?tggattgcac 60

gataacttta?ccgcgtctct?ccaaccggat?tgatgaaact?atcaacaggg?ccccgcatta 120

ttggcataac?cgacattttg?aaaaagtatc?gcgggcgaga?agagagcctt?tttcgacaac 180

aaacgtcggt?ctctttggtt?tgcatcgaga?gacatagata?cagttgaccg?cggcgcgaca 240

ctttttagga?gcgcagagga?cggtttctgc?ggcgatttga?tccgcaggcg?agcaaaggtc 300

taagacacct?ctgtttggaa?ctgcgcaggt?accagaactc?gaaactaatg?cgacattcga 360

gcgacgatga?tatatgtcaa?ctatatcctg?ggacttctgt?ccctcttaca?caccgctgta 420

gccacagctc?ctgattatgt?cgtggtagac?caactgaaca?gcatccccga?cggatggaca 480

aaaggcgcag?ctcccccgcc?atttactccg?atgaagttct?ggttgtcgat?gcatcacgag 540

tacaaggcgg?acttcgagca?gaaagtcatc?gatatctcga?cacccggtca?ccgggattat 600

ggacggcata?tgaaacgcaa?cgatgtcatg?gcctttatgc?gcccatccga?tcaggtctca 660

aagatcatct?tctcttggct?tgagtcggag?catgttccac?caaatgccat?cgaagatcgc 720

ggggattggg?tcgccttcac?agtcccgttg?gcccaagcac?aatcaatgat?gaagaccgat 780

ttttacaact?tccaccacct?ggaaacaaac?acaacccaaa?ttaggaccct?caagtactcc 840

gttcccgagc?aagtcgatgc?tcatctgcaa?atgatccagc?caacgactcg?cttcggccga 900

cctaagacac?aaaccagcct?accgagcctc?atgccagtgt?cggttaacat?tgatgaaata 960

agcgaagact?gcttgacagg?cgtgacgccc?atttgccttc?gccagctcta?tggtttacct 1020

agcaccaagg?caagccccga?ctcgagaaac?gtcctcggaa?tttccggcta?tctggaccag 1080

tacgcgcgct?acagtgacct?cgacgagttt?ctagccgtat?actctccaaa?cagcgtagac 1140

gccgacttct?ccgtagtatc?gatcaacgga?ggccaaaacc?cacaaaactc?acaagaggga 1200

agcacagagg?ccagtctcga?catccaatac?gccctctcca?tggcatttga?cgctaacgcg 1260

actttctaca?ctaccgccgg?acgtgcgcca?tccgtctcag?actcgggtac?ggtgagcacc 1320

gacggctcga?ccaacgagcc?gtatctcgaa?cagctccagt?atctggtggg?tcttccggac 1380

gaggatcttc?ctgcagtgct?tagcacgtct?tacggcgagg?atgagcaaag?tctgccggag 1440

gaatacacag?aagccacgtg?caatttattt?gcccaattag?gtgcacgcgg?ggtctcggtg 1500

atcttcagca?gcggagactc?gggcgtcgga?ggatcgtgtg?tatctaacga?cggaagccag 1560

aggacccgct?ttcagcctat?cttcccggcg?tcgtgcccgt?ttgttacatc?cgtgggtggg 1620

actgagggcg?tcgggccgga?aaaggctgtg?gacttttcga?gtggagggtt?ctccgagcgc 1680

tttgctcgcc?cgtcgtacca?gaatgcgagt?gtggaagcat?accttgcccg?cttaggagat 1740

aaatgggatg?gattgtataa?tccagacgga?cggggtattc?ctgatgtgtc?ggcccaggct 1800

agcaactatg?taatcaggga?ccatgggcaa?tggctacaaa?ctgcgggaac?aaggttagtg 1860

gccctaccaa?gtcaacaatt?gaaaatcaaa?cgagctgata?atattacagt?gctgccgccc 1920

ctgtctttgc?agcagtcatc?tctcgactga?acgctgcacg?tctcgagcag?ggtaaaccta 1980

cactagggtt?tctgaatcct?tggctgtact?cactcgacca?gcaaggattt?acggatattg 2040

tagacggcgg?atcagtgggt?tgtgacgggt?caaatggagg?agctcttgtc?ccgtatgcca 2100

gttggaatgc?caccaaggga?tgggatccgg?ttactgggct?ggggacacct?ctgtatcaga 2160

ctctggagca?gttggcgcag?tctgcttagt?actgcggcgg?gatggcctat?tttgtgtcgt 2220

tgatgttttt?gtcccctaaa?tgtcaacgcc?attaattctt?ctcaagtgcg?attagatttc 2280

gtaaaacaga?agctggacaa?tatgcagaaa?ttagagtaca?aagctggaga?cagaaggtcg 2340

atctccagaa?tttgctaatg?gttgtggtac?tcacgtcagg?gtgctcgagc?acaccagcta 2400

actaccaact?ttacccgtta?attctaccgc?agtagtaatc?tagaaaatac?tagataagct 2460

gatcaagctt?gaaacaaata?agcttaccga?ccgagaccca?tacagcctcg?cagtacaaca 2520

acttactacg?ggtgagacat?cctcacccgt?ccgtaagatc?gaaaggatta?ctatacacgg 2580

agtaagcact 2590

<210>10

<211>3080

<212>DNA

＜213〉aspergillus niger

<400>10

ggtacagtag?tgggttttag?tttggatgta?tctggagaca?tcgatgcttg?ttcgccggcg 60

ctaatttggg?gccatcgctt?agggaagaaa?aagaaaggaa?ggatttcatc?tgccgcggca 120

tttttggtca?ggcattggca?atactccaag?cagcttgcca?aagtgagaaa?tgtgcttatg 180

aataatattg?agtaatagtt?aatcaattgt?cttacaggat?aatatgggaa?tagcattggc 240

attgcgtggt?tggcaggcag?catgttgcaa?attgcaacaa?cgcgatcccg?gcgatcgccg 300

tcggactccg?ggtgggaatc?agggttggag?acccattgct?tatcaacaac?agccgacgcc 360

agtcgttctg?gggtgcattt?gcagaatctt?tggacgaatc?agccttttgt?cagtatcggc 420

cggtccgaat?tgacccagct?tgctagccgt?tggagacatg?caatgatgcc?attgtctcgt 480

ccgataagca?agtgcggcca?atccctcgtt?ccttctggct?gtctcagaaa?agttataatg 540

tccgtctcgc?ctgcctagcg?aacaattcga?actctgtgtg?tgttgtccct?ctcaaggtct 600

ccagcatgcg?ttcttccggt?ctctacacag?cactcctgtg?ctccctggcc?gcctcgacca 660

acgcgattgt?ccatgaaaag?ctcgccgcgg?tcccctccgg?ctggcatcat?gtcgaagatg 720

ctggctccga?ccaccagata?agcttgtcga?tcgcgctggc?acgcaagaac?ctcgatcagc 780

ttgaatccaa?gctgaaagac?ttgtcaacac?ctggcgaatc?gcaatacggc?cagtggctgg 840

accaggagga?tgtcgacacg?ctgttcccgg?tggccagcga?caaggctgtg?attaactggc 900

tgcgcagcgc?caacatcacc?catatttccc?gccagggcag?cttggtgaac?tttgcgacca 960

cggtcgataa?ggtgaacaag?cttctcaacg?ccacctttgc?ctactaccaa?agcggctctt 1020

cccagagatt?gcgcacaaca?gagtactcca?tcccggatga?tctggtcgac?tcaatcgacc 1080

tcatctcccc?aacgaccttc?ttcggcaagg?aaaagaccac?tgctggtctg?aaccagcggg 1140

cgcaaaagat?tgacacccat?gtggccaaac?gctccaacag?ctcgtcctgt?gccgatgtca 1200

tcacgctgtc?ctgcctgaag?gagatgtaca?attttggcaa?ctacactccc?agcgcctcgt 1260

cgggcagcaa?gctgggcttc?ggcagcttcc?tgaacgaatc?cgcctcgtat?tctgaccttg 1320

ccaagttcga?gaagctgttt?aacctgccct?cccagagctt?ttccgtggag?ttggtcaacg 1380

gcggtgtcaa?tgatcagaat?caatcgacgg?cttccttgac?cgaggcggac?ctcgatgtgg 1440

aattgctcgt?cggagttgct?catcccctcc?cggtgactga?gttcatcact?tctggcgaac 1500

cgtgagtatt?gaattcctag?acagagcttg?aatcgaagct?aattgtgtag?tcctttcatt 1560

cccgaccccg?atgagccgag?tgccgccgac?aacgagaacg?agccttacct?ccagtactat 1620

gagtaccttc?tctccaagcc?caactcggct?ctgccccaag?tgatttccaa?ctcctatggt 1680

gacgacgaac?aggtacgacc?ccacattccc?ccttccctgt?atgtagatac?taaccggacc 1740

agaccgttcc?agagtactac?gccaagcgag?tctgcaacct?gatcggactt?gttggcctgc 1800

gcggcatcag?tgtcctcgag?tcgtccggtg?acgaaggtac?acttacttcc?cctgtcctat 1860

tccttgcatc?gaaaataccc?taacagcagc?acaggtatcg?gatctggctg?ccgaaccacc 1920

gacggcacca?accgaaccca?attcaacccc?atcttcccgg?ccacctgtcc?ctacgtgact 1980

gccgtgggag?gaacaatgtc?ctatgccccc?gaaatcgcct?gggaagccag?ttccggcgga 2040

ttcagcaact?acttcgagcg?ggcgtggttc?cagaaggaag?ctgtgcagaa?ctacctggcg 2100

caccacatca?ccaacgagac?caagcagtac?tactcgcaat?tcgccaactt?tagcggtcgc 2160

ggatttcctg?acgttgctgc?ccatagcttt?gagccttcgt?gagtccattc?ccagcatcat 2220

gataatacga?aatagggtct?aatgacatct?gcagatatga?ggttatcttc?tacggcgccc 2280

gctacggctc?cggcggtacc?tcagccgcgt?gtcccctttt?ctctgcgcta?gtgggcatgc 2340

tgaacgatgc?tcgtctgcgg?gcgggcaagt?ccacgctggg?tttcttgaac?cccctgctct 2400

atagcaaggg?gtacagagcg?ttgactgatg?tgacgggggg?ccagtcgatc?ggatgcaatg 2460

gcattgatcc?gcagaatgat?gagactgttg?ccggcgcggg?cattatcccg?tgggcgcact 2520

ggaatgccac?ggtcggatgg?gatccggtga?ctggattggg?acttcctgac?tttgagaagt 2580

tgaggcagtt?ggtgctgtcg?ttgtagatgt?atactatata?tatggtatga?gattatgtat 2640

gtgatatgtg?atattatgtg?agagagaatg?gtttagactg?tgcgtcatat?acatggacag 2700

ttcattttct?cattaaacga?gcaccttcat?acggtaagga?cctcagaggt?tcctcccatt 2760

gttatgaccg?cttcccttct?ttctagagat?acatgcttcc?ccaccccgcc?tcaacgcgac 2820

cgtctacgga?caccagtcag?aagaccccaa?caacccacta?ttagtggcta?gtaaggacga 2880

catgcataat?tactccgtga?aacccccgaa?attaagcccg?acaggggcat?agagaaaccg 2940

atccaatcac?ttaacttacc?ccgcttctag?cacggcatag?ggctgagcga?tgcgatggtg 3000

caatggccac?gccgttgccg?attagccgta?gggccagttg?tgaatttccg?ggatcaccct 3060

acaacacatg?cttcatgcgt 3080

<210>11

<211>1890

<212>DNA

＜213〉aspergillus niger

<400>11

gaggatgata?gtctgatggc?cattccgctt?gaggcaaagt?gtgccccaat?gtcacgcggt 60

cagctagtct?tctgcaggtc?tgcagttgga?caggggaact?tgagtgctat?tatcgccaag 120

gtgaatgttc?tctaccgctt?tgataccaga?gtgatcgtcc?cttttgccgt?cactcttggt 180

gttgcgaagc?agatgtcgtt?cgtttgcata?tgggagccga?agatccacgc?agggtgcttg 240

aaaagacata?ctgcgatcct?cccgtttcca?agaatgcttg?tacacttgaa?gtttttgtca 300

agatcccccc?gcctggccag?tcccgagcta?agcgaaatga?gctttccctc?aatgctgcag 360

acaggagaca?ccatcgcttt?ggcgccttgg?caccatcagc?caccgtcggt?cctatcatca 420

tcgccctggt?caatagggaa?cgcgtcaatt?gcgtatctgg?gtagtgttat?ctgctcgata 480

ttgcaccggc?agagcaggaa?tcgggattgg?cggatgtaga?gaaaattcag?cttcaaggag 540

gggggaaggg?agacgctgaa?atggtataaa?accacgtcca?attccctacc?agatagccct 600

catccagcag?catcaaaagc?atcttccact?cagactccaa?gcagctccca?gtccctcttc 660

aattcattac?cttccaaaca?tcatcccatc?aagatgaaga?ctactgctct?cttgaccgcc 720

ggcctgctgg?ccaccactgc?tatggccgct?cctctgacgg?ccaagcgcca?ggctgctcgg 780

gccaagcgct?ccacgaaccg?ccagagcaac?cctcccttca?agcctggcac?caacgaggtc 840

ctcgccctta?acggcaccaa?gaatgtggag?tacagctcca?actgggccgg?tgccgtcctc 900

attggcactg?gttacactgc?cgtgaccgcc?gagttcgtcg?tgcccacccc?ctccgtgccc 960

tccggtggct?cgagccgcga?ggagtactgt?gcctccgcct?gggtgggcat?tgacggtgac 1020

acctgtgaca?ctgctatcct?ccagaccggt?gtggactttt?gtgtccaggg?cagcgaggtg 1080

agcttcgatg?cctggtacga?gtggtacccc?gactacgcct?acgacttcag?cggcatctcc 1140

atctcggccg?gtgataccat?caaggtcacc?gtcgatgcca?gcagcgacac?caccggtact 1200

gccacgattg?agaacgtgag?cactggtacc?acggtcaccc?acagcttcac?gggcggtgtt 1260

gatggtgatc?tgtgtgagta?caacgctgag?tggatcgtcg?aggacttcga?ggaggatgac 1320

tccctcgttc?cctttgccga?ctttggcacc?gtgactttca?ccagctgctc?cgctaccaag 1380

gatggttcct?ctgttggccc?tgaggatgct?accatcatcg?acatcgagca?gaatgaggtg 1440

ctgacctccg?tttccgtctc?cagtagcgag?gtcgttgtca?agtacgtcta?agacgttgga 1500

tgtatggggt?tcgggattgt?tgatgccccg?atgggtgtgc?tttcgacggc?aatggtgaga 1560

tgagtgatgg?aaatgagagg?ttggtctttt?ggcccggggc?tagttttctc?ttggtactcc 1620

tgaaaagcca?atagttcaaa?tgtctttatc?ttgttagttt?gatgttatag?tttgccttga 1680

gttgatttaa?ttgaaccaac?gttggatcat?cgtatccctt?agcgcaaata?aaatattacg 1740

tacgcctttt?aaaatattat?gtatgtctgt?taaggttctg?actgttcaga?tttatgtcaa 1800

acaacagcaa?atcattggta?atccaggact?cggggatatg?agcttcagac?attcacccca 1860

ttactgcatg?actatgaccg?tgtctttagc 1890

<210>12

<211>3080

<212>DNA

＜213〉aspergillus niger

<400>12

ttgttgtgat?tgctaagaga?catcacattg?ctcatatgag?tttgattgcg?actgttaata 60

ttaatgtccg?actgggagga?aatgagtggc?gttatccagg?cagctgcatg?caatactaca 120

ttcccctcac?gagggaaatc?tggccatctc?ggaaatcaat?ggtgtcatcc?gagttgattc 180

ccctcaataa?tggtcctgat?aagccactat?tgctgcagaa?cgcctatctg?tgccgttagt 240

gcatgcatag?tgtccccagt?tctttttctg?ccgattaatc?tattgccaga?cggactccgt 300

cgcgcttccg?aatgccggga?accgcggcta?agctttgctg?tagctttgct?gcaggaacat 360

ctgcatcttt?caaaatatct?tcagaggagc?cgatcgctga?gtttctcaag?agattagatg 420

aatgttcgta?cttggccgca?atataagctt?catggcggct?tcaaccttcc?atgagaaata 480

agaataaggc?tgccggggat?ggcctcaccg?tgtccctgca?tgcaagggat?gcataatgca 540

ccgtgacctg?ttccaccacg?ccggagcccg?cccgccctcc?gacggcccaa?acccactgca 600

gaatggcttg?attaagttcc?ccttctataa?ctaaccggtt?gttcctcgtc?gttgtctgct 660

ctgctctcca?cccagggtcg?tttttgcttt?tgcctcctta?accatggtcg?ccttttcccg 720

catctcggca?ggcttcgccc?ttgccgcccc?tgccctggcc?agcgtcgtcc?tggagaccgt 780

caagtctgtt?cccagcgact?ggaagctcgt?ggaggctgct?gataccagct?ccacaatttc 840

tttgtccgtt?gctctggcgc?gtcagaacct?ggaccagttg?gaggagaagc?tcctggccgt 900

gtccacccct?ggcaaggaca?cctacggcca?gttcttggat?ctggacgaca?tcaatgagca 960

gtttcctctc?gcagatgacg?ctgctgttgt?ggcttggctg?aagaaggcag?gcgtcaccca 1020

gatccataag?gagggtggtc?tgctgaactt?tgcgaccact?gtgggcacag?ccaaccagct 1080

tctcaacacc?accttctcgg?tgtacaagag?cggatctacc?cagaagctgc?gcacaacgca 1140

atactctgtt?ccggatgagc?tgaccgggtc?cattgatctc?atctcgccga?ctgttttctt 1200

tggaaagtcc?aacgctgcgc?gctcggcggc?cgtgcgtgct?tcgcagacta?ccaaggagac 1260

cagcagaaag?aagagcagta?atgtgtgcga?gtacatcact?ccggattgcc?tcaaagagca 1320

gtatagcatt?gactatacgc?ccgaggcatc?gtcgggaagt?cgtgttgggt?ttggcagttt 1380

cttgaacgag?tcggccttgt?actcggattt?ggatctgttc?acccagtact?ttgacattcc 1440

ccagcagagt?ttcactgttg?agactatcaa?cgggggaatc?aacaaccagg?agaatgatcc 1500

ggatggtgaa?gccgatctcg?atgtccagaa?catcgtgggc?atctcgcatc?ccttgccggt 1560

gacggagtac?attaccggag?gatctccgtg?agtgttccca?aagatgcaat?tgaattaaag 1620

ctaatggttc?agtccattca?ttcccgacgt?cgagactact?accgacgaga?acgagcctta 1680

cctgcagtac?tacgagtatc?tgctggccaa?gaccaacgac?gagctgccac?tggttatcag 1740

caactcgtac?ggcgatgacg?aagatgtaag?catccctgcc?tcccacacaa?atcgcctgct 1800

gacagaatag?accgttccca?ttgcctacgc?cacccgcgta?tgcaacctca?tcggcctgat 1860

gggcacacgt?ggtatctcca?tcctcgagtc?ttccggcgac?tctggtacgt?tgtaccccat 1920

atatattgca?tcaagtcccg?actgacaaat?acaggtgtgg?gcggcgcatg?catgtccaac 1980

gacggcaccg?acaagaccga?attcaccccc?atgttcccag?gaacatgccc?gtacatcacc 2040

gcggtcggcg?gcacccaaga?cgtgcccgaa?gtcgcctggg?tggacagctc?cggcggcttc 2100

agcaactact?tctcgcagcc?gtcgtaccag?tcggatcagg?tggagaccta?cctggacaag 2160

tacatctctg?cctcgacgaa?gaagtactac?gagcagtaca?ccaacttcag?cggtcgcgcg 2220

ttccctgacg?tgtctgcgtt?tgcaggttct?ccttagtatg?tatccatccc?agatgattgt 2280

atggacatct?gctaatgtcc?gacagctacg?aaacttatat?tgatggtcag?ctcggccttg 2340

tggcgggtac?ttctggcgct?agccctgtgt?ttgcggggat?cgtcgcgctg?ctgaacgatg 2400

cccgtctgcg?ggccaacaag?acatccttgg?gcttcctgaa?cccttggctg?tactcgagcg 2460

gctacaagag?cctgaatgac?attaccagtg?gcgaggcagt?gggctgccaa?ggcgatgtgg 2520

agggcgctgg?agtcattcct?tgggcgagct?ggaatgccac?gacgggatgg?gatccggcga 2580

cagggctggg?aacgcctaat?tttgccaagc?tgaaggaggc?ggttcttgcg?ttgtaagcag 2640

gaggatactg?agtggacgtg?cggagtgaag?gatatgtgca?aggcgttaac?ttataatagc 2700

ctgtgtgtgg?actatagaat?catctacagc?ccacaaccaa?ttagcttctg?catagtcacc 2760

acccttaaac?taaggataat?caattgttta?ccgctgtcag?tctcaaatcc?gtcatgtcat 2820

ggaccctgtt?cgatcaactt?gaaacaagct?taatctacca?gcggcggtga?tgaaaccgcc 2880

agcggacact?tgtttcagtt?acccgcaagc?ctgtatccag?tatgcaagcc?ctacttaaac 2940

ccttcaccac?cctgattatt?ctactctctc?tctctctcca?accacaccat?tcttttcttc 3000

attgtgccgg?tcacatccat?tctttacttt?cattctcctc?ccttctcttctcttctctac 3060

ttccccaagt?cactcattat 3080

<210>13

<211>3598

<212>DNA

＜213〉aspergillus niger

<400>13

accggagcag?aggaacaagc?ggagaagctg?aaacgtatga?atgctgagta?cgagcaaaag 60

ttccctggct?tgcggtttgt?gtatgttctc?tcagtatacc?caacgacttg?tgttggtctg 120

acatatctcc?ctggcatagt?acttttgtaa?acggtcgcaa?ccgggatgtc?atcatggagg 180

agatgcgtga?gaggattgac?cggggcaatg?ccgatcgtga?ggtggaggag?attatacagg 240

tgagatggct?gtctacccaa?acacggagca?gtgctgacag?ttcgctcctt?taaaggcaat 300

gtgcgatatt?gcaaaggatc?gggcgcggaa?actggagcag?tccgctaaga?tatagttcac 360

atactgcaag?catatattta?aacaatgaat?acccttcgat?ttcagcaggc?gctgcgacat 420

ctccttgctg?aatcgctgct?ttacaagcca?tatttcggtc?catgcgattc?atctatggag 480

cactctgcct?ctgtccggaa?acaatggctg?gtaatctgca?tggaacttgg?tgattccgtt 540

agtataaagt?gaccaactag?tgccggcgaa?ttatgggaag?cctgccttgt?cttacgacgg 600

gacgatcttc?cgtcgaacgc?gtgctaccag?cattttcata?gtatgacggg?tccgtatttg 660

atttactatc?gatggtaaaa?cgcctcctct?atggggtccc?ctagcactcg?gcaggccagc 720

ttgtgagaca?acccgggagg?tcagccatta?ttaggccaat?gagagctggc?aggttggaga 780

ggttgtactt?cgacaatgtt?ggacagccat?gcaacgtcgg?acaatagctt?tgatagggaa 840

tatcctaagg?gcgaaccagg?agggataggg?gtaggtatgg?ctgtatcgat?tagtagtccc 900

tctttcaccc?agaacaaact?ctagtatata?tagtagtaaa?tgttccgcgg?atggccaatg 960

accccaaatc?atctttcatc?ccatccacgc?atgagcaaac?atgcatggtc?tgcgcctagt 1020

atgcagcata?gggacattgc?ctttggttat?cctggcatat?ccggcggctt?cattgcatac 1080

aacttcagca?gccgtggact?tggactccct?tcgtctgacc?tctaactccg?aatacgtcaa 1140

ttctgtccat?gtagacacga?atcgatcagt?cgcagtgtcc?gctgaagaac?attataccga 1200

tacagcagct?cgactggttc?agaacattgt?tcctggagcg?agctttcgtc?tcatcgatga 1260

ccactttgtc?ggcgacaatg?gagttgcaca?tgtatacttc?cgccaaacgc?tccatggtat 1320

tgacattgac?aatgcggatt?tcaatgttaa?tgtctgctcc?ccaattactc?tgttcggaag 1380

gaagcttact?gttacagatt?ggaaaagatg?gactggtctt?gtctttcgga?cattcgttct 1440

tcacaggcgc?gttgccgagc?agccatctgg?acaataccaa?cgttttgagt?ccggaggctg 1500

cacttagagg?agcaagggac?gctatacagc?ttccactgac?tattgacaat?gtttctactg 1560

aagctgcaga?ggggcggaac?gagtacatat?tcagagaggc?agtgggagcg?gtatctgacc 1620

ccaaagctaa?gctagtctac?cttgtcaagc?cagaagggac?tctggcgctc?acctggagga 1680

tagaaacaga?catgtatgag?cactggctac?tgacatacat?tgatgcagag?actaccactg 1740

tccacggcgt?ggttgactat?gtcgcagacg?cgacatatca?agtttagtga?gtggctctcc 1800

ccatcgaata?acgaagctaa?gacaacagtc?cctggggcac?aaacgatcca?gcagaaggac 1860

atcgcaccat?tgtcaccgac?ccctgggacc?tatccgcatc?cgcatacacc?tggataagcg 1920

atggacggga?caactacacc?acaaccagag?gcaacaatgc?catcgcacac?tggaatccga 1980

ccggcggtgg?ctcctatctc?tacaacctac?gtccatccga?ccccaacttg?aatttccaat 2040

ggccatactc?cccaaacatg?tccccacccc?gatcatacat?caacgcctcc?atcgtccaac 2100

tcttctacac?agcaaacgcc?taccacgacc?tcctctatac?actcggcttc?accgaatccg 2160

ctggcaactt?ccaatggaat?aacagcgccc?acggcggccg?agacaaagac?tacgtgatcc 2220

tcaacgcaca?agacggctcc?gggttcagca?acgcaaactt?tgcaacccca?cccgatggta 2280

tccccggccg?tatgcgcatg?tacatctgga?tcgagtctac?tccgtcgcgt?gatggaagtt 2340

ttgacgcggg?cattgtaatt?cacgaataca?ctcacggtgg?taagcatctc?cccagaagat 2400

ggaagtccta?atctaacaaa?ccagtatcca?atcgtctcac?cggcggctcc?cacaacgccg 2460

gatgcctcag?cgccctcgaa?tcgggtggca?tgggcgaagg?ctggggcgac?tttatggcga 2520

cggccatccg?aatcaagccc?aacgatacac?gcacaacgtc?ttacactatg?ggtgcatggg 2580

cagataatga?taaatgtggt?gtccgggact?atccttattc?tacctccttt?actgagaacc 2640

ctttgaacta?tacgagcgtg?aataccatga?acggcgtgca?cgccatcgga?actgtctggg 2700

caaccatgct?atacgaggtc?ttgtggaacc?tcatcgacaa?gtacgggaag?aatgatgggt 2760

cgaggccggt?gtttagaaac?ggggtgccta?cagatggaaa?gtacttgatg?atgaagttgg 2820

tggtggatgg?gatggcactg?taagtgatgc?acagaatagc?ttctttcacc?tttgtaatgt 2880

ccgtggtatc?ctgactaatg?gactgaaggc?aaccatgtaa?tccgaacttc?gtgcaagcca 2940

gggacgcgat?ccttgacgca?gacattgtgt?tgactggcgg?gaagaatcgc?tgtgagatct 3000

ggagggggtt?tgcgaagaga?ggattggggc?aaggagcggc?tcatagtagt?ttaaattgga 3060

tgcggagggg?gagtacactt?cttcctacgg?gatgttagtc?ttgcacaggt?cgttcttggt 3120

gagagatgag?tggagatgcc?tggtttatta?gaataggact?aattatctag?cactaataca 3180

atatcgacag?atctaaacag?taagtataaa?tctataagtt?atcacacgca?taaaccccat 3240

caccccctag?tctacttcga?aacaaacacc?acaccaccaa?catcctcatc?ctcaccaaac 3300

acccctcccc?cgcataagaa?cctaaacaac?agccctcaac?tccatactct?gcgcccccct 3360

cgccacacac?aacaacaacc?cacccacgag?cagactcacc?cccgcgtata?tcgacagcgc 3420

cagaaaccca?tacttctgca?ccagcgcccc?ggaaataggt?atcccagcca?tagcactaca 3480

ctcccagata?agatatgatt?agcaaaatcc?acccttactc?cccaagaaga?tcaattcaaa 3540

ataaatctaa?tgtgcatacc?tcaaagccgt?agcccccata?acaaccccca?ccgccaac 3598

<210>14

<211>2847

<212>DNA

＜213〉aspergillus niger

<400>14

cttttggctt?gtgatcttga?ttgctagaga?tgtatatcct?cacggatacc?gccggagtgc 60

gccatttctg?gttaccttct?ctttcccttt?ttgtctcgat?cgtgaggcgg?aacgcaggat 120

gaagacacgg?cttctccatc?gcggcccacc?aaccaacaat?gtccttggac?gcccaactct 180

ccatctactg?gtcattggtc?caatgcagag?actccgtcga?gctcaaatgg?gccggccaac 240

cccgagtcgt?caggggcagc?ggcagcgacg?agctaaatta?gaccactgat?aagacgcgat 300

agtccaaagt?ctgaccgtca?cattgtgcca?ggcagataag?ttgaatcgtg?tgactggatg 360

ttggctaacg?tatggcgtct?ccggaggccc?gacggaccct?gcgcgatcgg?cggtggagcg 420

caatctaagg?acatccgcgc?ctaagatatc?tacccttcag?cagttcagcc?tagccctgca 480

gacttgtcgg?accagtgcta?tcgtgatcgg?cccccacggt?cgaatgagct?cttgtctctt 540

tccgtcagac?cctgccagtt?aatctgctat?ctactccgcg?gtaacatcgt?gcctgtctcc 600

actaaggcag?ggtccagggc?tgtatgtctt?actttgcacc?gagtcggccg?ccggttggct 660

ctgtcttggc?aattgcgaat?atcctcacgg?gcgacggacg?acacggattt?ggacggacat 720

gcggagatct?tcgtcggttt?attcctggaa?gggacatcat?ctccttccat?catgacggct 780

gccatagcgg?ggactctgag?acatttttgc?tctgaagagc?atggtcgact?tggatgatgg 840

aggagttgat?cgaggtcaat?gaggagaggc?ttgcaagtat?aagaagagac?tgctcgacca 900

gcagaatgga?tcttcttgtt?catcaaccaa?gagtccaagg?cttctttgtc?tggttctatc 960

tcttctccga?actctcttgc?ttgacattct?cgtggtcaaa?atggtcgtct?tcagcaaaac 1020

cgctgccctc?gttctgggtc?tgtcctccgc?cgtctctgcg?gcgccggctc?ctactcgcaa 1080

gggcttcacc?atcaaccaga?ttgcccggcc?tgccaacaag?acccgcacca?tcaacctgcc 1140

aggcatgtac?gcccgttccc?tggccaagtt?tggcggtacg?gtgccccaga?gcgtgaagga 1200

ggctgccagc?aagggtagtg?ccgtgaccac?gccccagaac?aatgacgagg?agtacctgac 1260

tcccgtcact?gtcggaaagt?ccaccctcca?tctggacttt?gacaccggat?ctgcagatct 1320

gtaagcttcc?ctgctcgggt?gttcgggcaa?atcgtgacta?acctggacta?gctgggtctt 1380

ctcggacgag?ctcccttcct?cggagcagac?cggtcacgat?ctgtacacgc?ctagctccag 1440

cgcgaccaag?ctgagcggct?acacttggga?catctcctac?ggtgacggca?gctcggccag 1500

cggagacgtg?taccgggata?ctgtcactgt?cggcggtgtc?accaccaaca?agcaggctgt 1560

tgaagcagcc?agcaagatca?gctccgagtt?cgttcagaac?acggccaatg?acggcctttt 1620

gggactggcc?tttagctcca?tcaacactgg?tgagtcaatc?ctacatcagc?cgggttgacc 1680

tacctgctga?ccgatacaca?gtccagccca?aggcgcagac?caccttcttc?gacaccgtca 1740

agtcccagct?ggactctccc?cttttcgccg?tgcagctgaa?gcacgacgcc?cccggtgttt 1800

acgactttgg?ctacatcgat?gactccaagt?acaccggttc?tatcacctac?acggatgccg 1860

atagctccca?gggttactgg?ggcttcagca?ccgacggcta?cagtatcggt?gacggcagct 1920

ccagctccag?cggcttcagc?gccattgctg?gtaagaaccg?ccttcattta?acacacaact 1980

tgtccacctc?tttactaact?agtgtataga?caccggtacc?accctcatcc?tcctcgatga 2040

cgaaatcgtc?tccgcctact?acgagcaggt?ttctggcgct?caggagagcg?aggaagccgg 2100

tggctacgtt?ttctcttgct?cgaccaaccc?ccctgacttc?actgtcgtga?ttggcgacta 2160

caaggccgtt?gttccgggca?agtacatcaa?ctacgctccc?atctcgactg?gcagctccac 2220

ctgctttggc?ggtatccaga?gcaacagcgg?tctgggactg?tccatcctgg?gtgatgtttt 2280

cttgaagagc?cagtacgtgg?tcttcaactc?tgagggccct?aagctgggat?tcgccgctca 2340

ggcttagatt?atccactgaa?gtggagtcta?tgatctgctg?attgatccct?cgacgatgaa 2400

ctacatgtgg?aaatgcatag?cagacgaggg?tgatggtgat?gatgttgatt?tgatgatgac 2460

ccgtacatac?ttgatgaagc?tcggtacata?tgcaaatgtg?actgtatcta?tgtgatgaat 2520

atatgtatcc?atctcatggc?ttttggctat?gagtgcagga?taaacacctg?aaccagtagt 2580

agtactttcc?cacctatatc?tactgcggtg?cctcgtccgg?cccaacatca?ccccagaggt 2640

ggccgcagag?gagtcttata?agatagctac?tatcagttac?aacacctctc?tgacagatgt 2700

gaaggagtac?aataaatcac?cgaaacacaa?attcaactaa?agtcggtaag?taataataat 2760

ttaagaccca?atccacgcaa?tgttaaacta?tctctggtgt?tgaaagatct?ctcccctggc 2820

aacacctagt?tgtgggagaa?ctgtgtt 2847

<210>15

<211>2899

<212>DNA

＜213〉aspergillus niger

<400>15

gcccaggtga?agaccggaca?ggaagcggat?aggtacggga?cattttctaa?tctacccgcg 60

atcgggacat?ggctaaccaa?gcatatagac?tcgaattcta?ccggtaaatc?aagtatggga 120

cgtgcatcag?gctggatatc?ggattacgca?aggcgaacag?ggggaccgtt?agctgtatta 180

tcaacatcta?ggctatttca?tattaggaca?acgactgacg?cattgggtat?tccgctgggg 240

tagtcttatc?ggttggggcc?aagtaccttg?tagaactgta?acccacgtta?ataccgccac 300

ttggctgggg?cggtgattta?gcatatgtaa?gctccagttg?gacggctacc?cgagcttccc 360

atgatctaca?ggagtacgtg?tctggctgtc?tgctgcctac?ttggtagaca?ggtcagcgat 420

aggtagatag?gacctgtccg?cagctgttgg?ctagtttggt?aaggcggttg?cgctagtttg 480

aagtaggcag?gcaccgggaa?cctaaggcgg?tcttacatca?tcacccgcgc?tcggattcgc 540

gtgatccgac?catcacgata?aggcctcagg?tagcaaggag?accttccaga?cagctctgaa 600

tgagactcaa?aggtagatat?aatgatggaa?agataggata?gctagatcag?gcttattgta 660

cctgatcgtt?aagagcctag?agaagatgta?cctggaagac?ctggcagcta?caatcacctg 720

gagcgataac?ccgtgacgat?ccccttgcca?aatgacgcag?ccgggctggc?caaccattgg 780

ctgcgacctg?gcaggtcgct?ccgcaaccag?cgccgcccgg?ctccaagtca?cccgcatcac 840

tcttccctac?ccccagacct?cctcttttcc?cttgctatcc?tccatctctt?cttcatcgtt 900

ctttgtctct?atcatcattt?tctattcata?cgtgcatcat?tcagtcgttt?ggcccagtcc 960

atcatatccc?gctgggtagc?cgtttccgcc?gtcgcccatc?atgaagtcag?cctccttgct 1020

cacagcatcc?gtgctgttgg?gctgtgcctc?cgccgaggtt?cacaagctca?agcttaacaa 1080

ggtgcctctg?gaagagcagc?ttgtgagtgt?ggtctttcac?tgctttgttt?tttttagcta 1140

gttagcttca?aagaagctcc?agaaccattc?aaagctgatt?tcgtggtcta?tagtacacgc 1200

ataacatcga?cgcccatgtc?cgcgctctgg?gccagaagta?catgggtatc?cgcccgtcca 1260

tccacaaaga?gctggtcgag?gagaacccta?tcaatgacat?gagccgtcat?gatgttctgg 1320

tggacaactt?cctgaacgca?cagtgtatgg?agataccgtc?ttcttatggc?tgcaactgct 1380

gacccttcct?gccatagact?tctctgagat?cgagctgggt?actccccccc?agaagttcaa 1440

ggttgtcctg?gacactggca?gctcgaacct?ttgggttcct?tcgagcgaat?gcagctctat 1500

cgcctgctac?ctccacaaca?agtatgattc?gtctgcctcc?agtacgtatc?acaagaatgg 1560

cagtgaattc?gccatcaagt?acggctctgg?cagccttagc?ggattcattt?ctcaggacac 1620

cctgaagatt?ggcgacctga?aggtcaaggg?acaggacttc?gctgaggcga?ccaatgagcc 1680

tggccttgcc?tttgccttcg?gccggttcga?tggcattctc?ggcttgggtt?atgacaccat 1740

ctccgtgaac?aagattgttc?ctcccttcta?caacatgctt?gaccagggac?tcctcgacga 1800

gccggtcttt?gccttctacc?ttggagatac?caacaaggag?ggtgacgagt?ccgtggcgac 1860

cttcggtggt?gtcgacaagg?accactacac?cggcgagctg?atcaagattc?ccctccgtcg 1920

caaggcttac?tgggaggttg?agcttgacgc?cattgctctt?ggcgatgatg?ttgctgagat 1980

ggagaacacc?ggtgtcattc?tggacactgg?tacctccctg?attgctctgc?ctgctgacct 2040

ggctgagatg?atgtaagtcg?aattcttcgg?attcctgggt?tgaaaagaaa?tgctgctaac 2100

aaccttctag?caatgctcag?atcggtgcta?agaagggctg?gaccggccag?tacaccgttg 2160

actgcgacaa?gcgctcgtcc?ctgcccgatg?ttactttcac?ccttgccggc?cacaacttca 2220

ccatctcctc?gtatgactac?accttggagg?tgcagggctc?ttgcgtcagt?gccttcatgg 2280

gcatggactt?ccctgagccg?gttggtccct?tggccatttt?gggcgatgcg?ttcctgcgca 2340

agtggtacag?cgtgtatgac?ctgggcaaca?gcgctgttgg?tctggccaag?gccaagtaaa 2400

ttagttctgc?gggttgatgt?ggtatctatg?atgcagctgk?tgctgtcatt?attgcttctt 2460

gtagcttgat?ctatgatttt?tgcagacgaa?cacacgtgat?gttgtgaatg?gttttcctca 2520

tgtttgcagc?ggttgccgga?tagattctag?ggatcttcaa?tggaaagccg?gtgatattat 2580

ttgaccttta?tttgggcact?gagaatcttg?actgtatgaa?atatgatagt?aacaccttaa 2640

acatgaatgc?aaatggcgta?aaccgtgtga?tgcagtcaca?ataaccagca?accgcggtac 2700

cagccgaagt?ctgggccggc?gagtctcgcc?ccgcacaggc?caggcgccaa?acccaagcag 2760

cgctcttggc?agccaagcct?tcttcatcac?gctctcacca?cctcctccat?ccaggctttc 2820

tctctgctcg?gtctttgttt?catcatcctc?ccacgcctgc?ccttctattc?aaccgctctt 2880

cgatcttcat?atccgatcg 2899

<210>16

<211>2738

<212>DNA

＜213〉aspergillus niger

<400>16

gaggcatgag?gcatttctga?ggcccgctac?tccgcattct?gcagcatatc?gtctctgcgt 60

aggggaggtc?gaaaccagct?gtaggactcg?gcttcggtgt?atctgtaccg?actgactaga 120

aatcgctcaa?tcgtgtagta?tagctgtctc?tttgttcctc?acaacatgtc?tacgatatgc 180

tattaaaaaa?agcagaagat?ggagtcagag?ccacccggtt?agggccgggc?cgcccgggag 240

gagaacaaaa?tacgggacag?aatctcagtg?atgggggaga?agagagagtg?gcgacctgac 300

aattcacaca?cgacacgaat?aatagccgaa?actaacaaga?taaatcacat?cacatcatga 360

agaagacctg?cgtaatgatg?ataagcaatc?ccaccaataa?tacaatgcca?ttgatagtgg 420

ctgacctgaa?gcaattcggg?gaggagacgc?caagctcgac?gatcaccgga?gcttgaaaga 480

ccaacgagac?aagatgacag?gcccgtcgca?ccacgccact?aactgcccta?acagaaatcg 540

gcctgaatag?tgcgacgagt?gtcccggttc?tgggcctcca?cgataagata?agtcatgggc 600

ttatcgcgtc?atcggcgccg?atctcgcgat?cagctgaaac?caatcattca?atcaatttgc 660

atcacccgac?tgggggcgag?atttcagggc?cagctgaaac?ggtcggctgc?cgagattgtc 720

agtggatgat?gaatgttatg?ctggaagaga?gggggagaat?gacgtctcaa?ttctgggtca 780

cttactagtt?gactagccac?ctagtattta?gctgctagct?agggattcgg?tttaaaagcc 840

tggtggtttc?tctcttcttc?tcgtcatttt?ctcttcatct?catacccatt?cttcaaaact 900

cctccacttt?gatcaattat?cctccatcat?ggctaccaaa?atcaagctca?tccccaatct 960

caactacaag?cgctcaggca?ccaagtccta?cgtgcacttg?atgcgcaagt?accgcttcca 1020

tcccaccaag?cctggtccct?acactctcag?cagctccatc?caacagaccg?gtcgtccgta 1080

cactgaaaag?cccatcgggg?gtcgggccca?tatccggcag?ctggtgcgga?agaagagcac 1140

caccagcgat?gaggttggcg?aggttccggc?cgaagatgtg?cagaacgact?ccatgtatct 1200

ggcgaccgtg?gggatcggaa?ccccggcgca?gaacctgaag?ttggactttg?acactggttc 1260

agctgatctt?tgggtacacc?cccattatga?aagacctaat?atggaaacga?gcgtcactga 1320

cagatgtagg?tctggtccaa?caaactcccc?tcaacccttc?tatccgagaa?caagacccat 1380

gcgatcttcg?actcgtccaa?atcgagcacc?ttcaagacct?tggaaggtga?atcctggcaa 1440

atctcctacg?gagatggatc?ctccgcatca?gggagtgtgg?gcaccgacga?cgtcaacatt 1500

ggcggcgtag?tcgtcaagaa?ccaagccgtt?gagctggcag?agaagatgtc?cagcacattc 1560

gcccaaggcg?aaggggacgg?attgctcggt?ctagcattca?gcaacatcaa?cacggtacag 1620

ccaaagtccg?tgaaaacgcc?cgtcgagaac?atgatcctgc?aggatgacat?tcccaagtcg 1680

gctgagctgt?tcacggccaa?gctggatacc?tggcgggaca?ctgatgacga?gtcgttttac 1740

acctttggct?tcattgacca?ggatctggtg?aagacggcag?gtgaagaggt?ctactacacc 1800

cctgtcgata?acagtcaagg?cttctggcta?ttcaactcga?cctccgcgac?ggtaaatgga 1860

aagaccatta?accggtcggg?taacaccgcc?attgctgata?ccggtacgac?gctggccttg 1920

gtggacgatg?acacgtgtga?ggccatttat?agtgcaattg?acggcgccta?ttatgatcag 1980

gaagtacagg?gctggatcta?tccgaccgat?acggcgcagg?ataagctacc?cactgtgtcg 2040

tttgccgtgg?gtgaaaagca?gttcgtggtg?cagaaggagg?acctggcgtt?ttcggaggcg 2100

aagacgggct?atgtctatgg?aggaatccaa?agtcgtggtg?atatgaccat?ggacatcttg 2160

ggagacacat?ttttgaagag?tatttatgct?gtaagtgcat?tgctgttggc?gttaaggggt 2220

gatatcgaag?ctcactaact?ggattgcaga?tctttgatgt?cgggaacctg?cgctttggag 2280

ccgtccagcg?cgaggagttg?cgccagagcc?tgaagtcgga?gtagacgagt?ggctgaatct 2340

atcatggttt?gatgatgtta?tgtatttgca?tgtcccgttt?ttttggttaa?atcctagtgg 2400

cttttgagcc?ccgaagtatg?tacgcagtaa?atagcagtgc?aatatagtac?tccgtacctt 2460

gttatcagat?gctctggttt?gaagcacaaa?ggaagccatc?agtctcccaa?tcagagcttc 2520

cagcccgccc?cgtccttcca?tttccacaac?ctcagcgcca?tgaaacatgc?catccctgct 2580

cctaccataa?ccgccgcaaa?aagctgcccc?ttccagaaaa?cagcatgttc?cagatccaac 2640

aacgccccgg?caataggatt?tccaatgaga?atgccaagcc?ccgcgaacac?gaaagacatg 2700

cccatccacg?tccccacaac?agccatgtct?ggacacaa 2738

<210>17

<211>2349

<212>DNA

＜213〉aspergillus niger

<400>17

cgcgcactaa?ccctccacgt?attccaatat?accaaatctg?cccaaagcgc?cagccagctt 60

cctcaagcct?tgcggtcaga?taaggccctg?tacctagcta?gttgccgctg?ctcccggcgc 120

tgggccaagc?cgtcggacgt?ccgtcccccc?tctttccccc?tcctctcccc?tctccactgg 180

tggaacgatg?tctggctgtt?gccatcgttc?tcagaagcaa?cgccccctgg?atcgggtggc 240

tgtcgtacta?ttgcatgttc?gtccgcgcta?ctaggaaagt?ttttttccca?cccggagtat 300

ccgtgtttag?tccgcgggct?ggctgaccgg?ctagctggcc?gtgccagttg?ggtaaggttc 360

caagggagga?ccttactagg?tagaaacggg?atccaacaat?gaggggaaaa?gggcggatat 420

ggcttgccgg?gggttcattg?cggcctggac?gaagaaaggg?agatgatcac?taatgcaaca 480

caatcttggc?ttgcaaggaa?ttgcgctcca?accagaatgt?ctctgcgtag?ggatgccaat 540

tcgtgcgggc?catgctggat?ggatagtacg?ctgctccact?ctcgctcgac?cttttgcagt 600

ccacaatcgt?ttccccgtat?cgttgggcgg?gggcgttttt?ctgcagctat?ggttgctgct 660

gccccgacgg?tgaacctttc?tgcatccccg?gttttagtcg?attttagttg?gcgggcctgg 720

agattaaact?ccgtcggacg?aagaggagca?gtggtgtcat?cgtcggcgga?ttgcatgcta 780

tcggaagagc?atggaagagg?gaaaacatca?acttcatttg?caaaacgctc?gagcataaat 840

agaggcctgg?attccgccgt?tctggtgtct?tttcttcttc?atccagcatc?gcaagtctct 900

caagcatcgc?ctggttcgtt?cttctcactc?ttccaccacc?agccttgtca?ataagttagc 960

tcttcatctt?ttcgaagaaa?ccaattctcc?aaacgtcaaa?atgaagttct?ctaccatcct 1020

taccggctcc?ctcttcgcca?ctgccgctct?ggctgctcct?ctcactgaga?agcgccgtgc 1080

tcgcaaggag?gcccgcgccg?ctggcaagcg?ccacagcaac?cctccctaca?tccctggttc 1140

cgacaaggag?atcctcaagc?tgaacggcac?ctccaacgag?gattacagct?ccaactgggc 1200

tggtgccgtc?ctgatcggcg?acggctacac?caaggtcact?ggcgagttca?ctgtccccag 1260

tgtctctgct?ggatctagca?gctccagtgg?ctacggcggt?ggctacggct?actacaagaa 1320

caagagacaa?tccgaggagt?actgcgcctc?cgcttgggtt?ggtatcgacg?gtgacacctg 1380

cgagaccgct?attctccaga?ctggtgtcga?cttctgctac?gaggatggcc?agacttccta 1440

cgatgcctgg?tacgagtggt?accccgacta?cgcctacgac?ttcaacgaca?tcaccatctc 1500

cgagggtgac?accatcaagg?tcactgtcga?ggccaccagc?aagagcagcg?gtagcgccac 1560

cgttgagaac?ctgaccactg?gccagtccgt?cacccacacc?ttcagcggca?acgtcgaggg 1620

tgacctttgc?gagaccaacg?ccgagtggat?cgtcgaggac?ttcgagtctg?gtgactctct 1680

tgtggctttc?gctgacttcg?gctccgttac?cttcaccaat?gctgaggcta?ccagcgacgg 1740

ttccactgtc?ggcccctctg?acgctaccgt?tatggacatt?gagcaggatg?gcaccgtcct 1800

caccgagacc?tccgtctctg?gcgacagcgt?cactgtcacc?tacgtttaaa?tgcatctcta 1860

tgcatgagat?atcggtcgct?tcaatgtctt?cgagacgaag?acaaaccctg?gggatgaatg 1920

aaaaaatgag?tgatgagcta?tccggattga?tctgatcttg?ttgagttgtt?aattccgttt 1980

ctgttgatgt?ttttgaatga?ttatacctac?ttttaagtag?aagaaatgga?tgagcgcgtg 2040

catgctgaaa?atgactgtcc?ctgcttatat?tgtagaagat?cttccagaaa?gctgtgctgc 2100

cgatctgaag?atctgaagat?cactagtgag?atctcgcagc?tcggctgtgt?aagtgcattt 2160

gctctgtcga?tcataacttt?gtaaaagctt?gtatgcatag?cagacatctg?tcgattattt 2220

agatgcttcg?atttgatcat?ctttactaga?atccccattc?gagtagagct?tcagagcgtc 2280

gggtggaaat?atcgggtcgt?ggatggtatc?ggagaagtct?cacaacatga?acgaaagatc 2340

cgcggtata 2349

<210>18

<211>1495

<212>DNA

＜213〉aspergillus niger

<400>18

tgattgtgca?gacttcctca?tcatcgaagg?agacaacaat?gaaaccccag?ggagacacgg 60

aagcaggaaa?acagactgcg?gccggcgaga?aagacacgat?gtgacgaagg?cttatctcgt 120

gacgccaaga?caatgcgggg?aaagctgccc?ttccaagacc?caactggcct?gcctctttct 180

cattacaacc?tcatcgcctt?gattgacttt?ctggaccttg?gtggcttgga?ggccggtcgc 240

atcgagcttg?ctactcgcta?cttgctttgt?ttcgatgact?ggctttcgtt?gatcgcaaca 300

ttctttcttg?gtatttttgt?agggatagct?agttgcttat?catgggagat?tacggccccg 360

gagtgtcgtc?actcacggca?cagctacctg?gaaatccgcc?tgtctctgaa?acagatcagg 420

atgagatctc?agtacttgta?acgggctttg?gggtaagctt?ccagtgtcca?taccattctc 480

ccaatatcgc?aactcacatg?ttctacagcc?attcaagtct?aatctagtga?acgcctcata 540

tctgatagcc?tcgtccctac?caccctcttt?cacattctca?cctgcatctt?cagacggctc 600

tgatgctgtt?ccccgtcgag?tttcgataaa?tgtccatcct?tcacccatac?ccgttgcata 660

ttcatcggtg?cggacgaccc?tccccgtcat?tctcgatgac?tatgccaaga?cgcacggagg 720

ccgacgccca?gacatcgtca?tacacattgg?catagcagca?atgaggaact?actattccgt 780

ggagacgcag?gctcaccgtg?atgggtatct?gatgtccgac?atcaaaggca?gatccgggta 840

cgaggatggc?gagaagctgt?ggagggagct?cgacttgcca?ctggtgctta?gggctggccc 900

ttcagaggga?cacgcctcgg?agaagaaaca?tctcagcccc?cgtccaccgg?acgaagattt 960

cctagcagca?tggaagacat?tttgccctcc?agaaaccgat?gcgcggatct?ccactgatgc 1020

cggacgttat?ctctgcgagt?tcatcctgta?caccagcttg?gcactggcat?accaggcggg 1080

tgaggatcgc?aatgtcacct?tcttccatgt?tcccgcgtca?tgcttggatg?aggatataga 1140

gacgggcaag?gaggttgccg?tcgcgctaat?caaggctctt?gtgactagct?ggagtgagca 1200

gcagcacagc?gttccctagt?tctgaatgac?tttttcaatc?ttctcggagt?tgtgacattg 1260

catgtcccag?cagttttggt?ttatcggtgg?tcctatctga?gtactatatt?tttttggcaa 1320

tattttgcat?tagtgaatac?atatgggcgc?ctgatgggtt?atgatcgcat?cacacctggc 1380

gccgcataga?ttttgggcat?aaggagtgtt?ggtgtaagat?caccatcaat?ttattactgg 1440

tgttggattt?gtgtagggat?agaaccaata?tagattaaat?tctcacgcca?tacat 1495

<210>19

<211>2501

<212>DNA

＜213〉aspergillus niger

<400>19

gcgtcttcgg?caagcggaag?ggaccccgca?aaagctacgc?attgccattg?ttcaggaacc 60

cactaaataa?acacacggac?atgtggtttt?ggctgcccgt?gcgccccgaa?gccgcagccc 120

gaacggaggc?attaaccctg?atgcgtccgt?tgattgttat?ggtcttgtcc?aacataacgg 180

ggaatttctg?accgcacacg?atgccaggca?acccatttta?tcacggctga?gcatgttgat 240

aggtgttgaa?ttcattttcc?gtaggcagac?gtctcctaga?gcaatttctg?ctgtatggca 300

gacattcgtg?accgaaaccg?tctcactcac?ggtctaccat?ggtagttatg?cttcgtacat 360

aagacaagaa?aggaatgcct?tcaatcatgg?caagaaggga?atttggtaat?ttgggtgcta 420

ctgccgtctt?cctttgttgt?gaaccttctc?cattacacac?agtgtccctt?gaaggccccg 480

tatgccttca?tactccgcca?caatagccgc?catcggggct?ttcctgcttt?cgcagcccgt 540

catgggctca?aggcagggaa?aggccccctt?tggctggggt?actcagtcac?ttgctcactt 600

tggtatcaac?ccagaccttg?ggttgcacaa?ccagcagaac?ctcaactccc?tcatttcaca 660

ttcagcgatg?gccactgcgt?tggagacgga?atatgccacc?gtatgaaaag?caccagccct 720

ggctcttagt?cctgatcact?gattgtagac?tcagatccct?attgaccata?acaacgcatc 780

ggctggcact?tatcaaaatc?ggttctgggt?cagcgatgaa?ttctatcagc?ctggcaaccc 840

gatatttgtg?tacgataccg?gggagtcgga?tggcggatcg?atagcccagt?cctacctaac 900

ctccactctc?tccttcttca?gagaattcct?gatcgaattc?aacgccatgg?gaatcgcctg 960

ggagcacaga?tactatggaa?actcgacccc?ggctcccgta?tcctatgaaa?ctccacccga 1020

ggcatggcaa?tacctcacca?ccaagcaggc?gctcgcggac?cttccgtact?ttgctagtaa 1080

ctttagccgc?gagaagtatc?ctgacatgga?cctgacgccg?cagggcacgc?cgtggatcat 1140

ggtgggcggc?tcgtacgcag?ggattcgtgc?tgcattaact?cgcaaggagt?acccagagac 1200

gatattcgca?gccttttcct?catcgtctcc?ggtggaagca?caggtcaata?tgagcgcgta 1260

ttacgaccaa?gtctatcgtg?gcatggttgc?cagcggatgg?accaactgct?cggcagatat 1320

ccacgctgct?ctggaatata?ttgacgatca?actttcggat?gaagatacag?ctacctcggt 1380

caaacaactt?ttcttcggat?ctggcgccga?gaccaactcc?aacggtgatt?tcactgcagc 1440

gctaactgcc?atctacggct?acttccaaag?ttatggtatg?gcgggaggta?ttggaggtct 1500

aggcgcattc?tgcgagtatc?tcgaaattga?tcccaagacg?aacgggacta?caggaccgga 1560

tggccttgcc?cctacgtatg?gcggccagta?tgtcgccgaa?cgatgggccg?catggccaac 1620

ctttctcgag?ctggtcaatc?tgaatatggg?gaccaactgc?gggcctcagg?acgcgtctca 1680

gccaattgac?tgtgactttt?ccaagccata?cggcgatccc?tcggccatca?cttggacttg 1740

gcaatactgc?agcgaatggg?ggttcttcca?ggcgaacaac?gatgggccgc?actcgctggc 1800

ctcgcgatat?cagtcggtgg?aataccagca?agaagtatgt?aaccggcagt?tccccgatgc 1860

agtggacaag?ggactgctgc?ctccgtcgcc?gcgggcggat?gatgtcaacc?aagagtttgg 1920

gggatggacg?atccgcccgt?ccaatgttta?cttcagcgga?ggagaattcg?atccgtggcg 1980

atcattgtcc?attctgtcga?cagaagattt?cgcacctcaa?ggggtggagt?ttacgagcgc 2040

gatcccagcc?tgtggggtgc?agaccaatga?ggacaccgtc?tttggatacg?tcatgcagaa 2100

ctcggaacat?tgctttgact?ttcaagcgac?gccgaccgtg?gggaagttat?cacgcggcat 2160

cttcacatcc?gccttgttgc?aatggctcga?atgttttgga?cagaactcaa?gccaatccag 2220

gtgatctggg?gggccagggg?gtgtgtgggt?gtgtaggtgt?gggtgtgttt?ggttgtgctg 2280

gagcctggag?ccaggataaa?gaagatagga?caaggatgac?tgagtggatc?ctgggatgct 2340

cctacttact?tagaaaggta?cagggcgcgt?cacggcgaag?gtagactcgt?accccagatt 2400

agatgatcaa?cgagaagcct?cggaggtttt?ggatcaacgt?tgggttttag?cgtcacgtcc 2460

aaagtcctgc?atgcaggtgc?cgcttttccc?ttttgggaga?t 2501

<210>20

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>20

agagtccgcc?ttgacaatgt?ctaagaaagc?atgctgagcc?ccctccccca?gtgtttatag 60

gtgagtagga?atggagtata?gtgcttgagt?gctttcacgc?tggtaaacgg?acccgctggg 120

cggctagact?gccttgtttt?cggtctagga?accattcaag?attcaacggc?tacaagtcaa 180

gtcagaccat?ctatcaatag?tgcgagatag?cagagagtcg?gaccggagtc?tcacaggcat 240

ccttggcggt?cgaggagtga?tgagttgtgc?gaccagcgcg?tgttccttat?ttaagccgcg 300

cgcgcacctc?tgacgcaacg?gtctcaaagg?ggggtctcac?ctcttgcatg?ttcggctgca 360

ttcaattcga?cctctccgtg?tctgtctagc?atcaccccaa?atcctatcgg?aatagtcctc 420

cggttaagtc?aacattgcaa?atcagtcctt?tcatttttgc?ccagctcagc?gctcggcctt 480

ctgtgaccct?tggagcctga?tcaattgccg?aaaggtgttc?gggcgggtca?gccttggaca 540

gctcatttgc?agtgcagagg?ttcatcagca?tcagctgtac?tacttgactt?cctatcatca 600

ttattgatta?ttatattata?tcctatcttc?tccgccaatc?ttccatcgcc?ggtagttttt 660

cgtagacaac?atgaagctct?caatagctct?tgcactcggc?gcaacggctt?cgacgggggt 720

gttggctgct?gttgtaccgc?agcaagaacc?gctgataacc?ccccaagatc?ccccaactca 780

tcatcatcag?gagaagttct?tgatcgagtt?ggctccttat?cagacgagat?gggttaccga 840

ggaagaaaag?tgggacttaa?aactggtata?gcaacattcc?ccattttatt?actgtcacaa 900

tagcacatct?cactgatcgc?tctgcgaatg?ttccattagg?atggcgtgaa?cttcatcgat 960

attactgaag?aacgaaacac?tgggttctac?ccaacgttgc?atgctggtag?ctatgttcac 1020

tatccgccga?cgatgaagca?tgcggagaag?gtggttcccc?ttctgcgggg?tctctccaag 1080

gacaacatgg?agcaaaacct?caacaaattt?acctcatttc?acactcgcta?ctataggtcg 1140

tccactggta?ttgagtccgc?aaagtggcta?tacagtaggg?tttcggatgt?cattgagcag 1200

tcgggtgcag?cagagtacgg?cgccactgtg?gagcagttcg?ctcactcatg?gggccaattc 1260

agtatcattg?ctcggatccc?aggccagact?aacaaaactg?ttgtcctggg?cgcacatcag 1320

gacagcatca?atcttttcct?cccctccatc?ctagctgcac?ctggtgccga?tgatgacgga 1380

agtggaaccg?tgactatact?cgaagctttg?cgtggtctgc?tgcagtcaga?cgccattgtc 1440

cggggcaacg?cttccaacac?aatcgaattc?cactggtact?cggcagagga?aggtggtatg 1500

cttggttcgc?aagccatatt?ctctcaatat?aagagagata?agcgagacat?caaggcgatg 1560

cttcaacagg?atatgactgg?ttatacccag?ggagctctgg?acgccggtcg?tcaagaagcc 1620

attgggatta?tggttgacta?cgttgatgag?ggactgacac?aattcctcaa?agatgtcact 1680

actgaggtaa?ggtcactccc?gctttccttc?tttgtgagac?atataactaa?cgattgcggt 1740

caaagtattg?tggtattggc?tacatcgaaa?ccagatgtgg?ctacgcctgt?tcggaccaca 1800

cgtccgcaag?caaatatggc?tatcccgcag?ctatggcgac?ggaatccgaa?atggaaaaca 1860

gcaacaagag?gatccacacg?actgatgaca?gcatccggta?tctaagcttc?gatcatatgc 1920

tggagcatgc?gaggttgaca?cttggcttcg?cttacgagct?ggcctttgct?caattctagt 1980

gtccttcatg?attttacgtt?gtaaccgggt?ctagcagata?attctggcta?actagtgagg 2040

cttatatgtg?ttcaggtttc?ctatgtcggt?ttattgtggc?attagacaag?attacagagt 2100

aaccaatact?ccattttgta?atggaataga?tgtctgaggc?ccaagtgttg?gcaaggaacc 2160

ttgcctatat?accacagatt?aatactattg?gctttggtga?ttcaagaata?ggcctatggc 2220

tggactttat?atatgttgac?attctatttt?gtaccggcaa?cagactctaa?cactagcacc 2280

taacaaataa?aacacgtgtg?actcttatgg?gtggcatgat?gatagtctgc?agaatggccc 2340

gataagataa?acgaaggaga?tcctactata?cccgattgag?tcacactagc?ctgaaacaga 2400

aagacggtca?ctccgccggg?gcgatcactt?ccactggctt?caatcttcga?gtcattcctg 2460

cggtctgttt?tctccttgtt?accactccct?ttgcccttca?gatcagatcc?tgaacggaga 2520

aagctcaccc?ttcatactac?ataatattct?acataacttg?cttggactga?attggcgata 2580

ttccgtcggt?cgctcctatc?tatcgcctac?gtcaccagcg?gtgcccctcc?aaggaagacc 2640

ccaccatcag?accttggatc 2660

<210>21

<211>2047

<212>DNA

＜213〉aspergillus niger

<400>21

ccgagctata?caaatgcgca?tacaaaaatg?gacagagtta?catttcaacg?ataaacatga 60

atgataagct?tccagcaatg?gcggccaagc?gatatgcaaa?ccgattttag?tacgcattcg 120

gtttatctgt?tatctctctc?ggagtaaact?gtatgctctt?cgccgaaacc?acaaaagtaa 180

gcggggaaac?gtatactacc?aatcaaacac?cccgagatgg?tgactccgaa?cgcagcagac 240

ctatttagat?atatttaggc?atgacaagta?tcaatggatg?acagaaacac?cgagacctgg 300

tacaatctaa?gttgaggaga?gatttgaatc?agaaagcagc?tcgcacattt?catcatgaga 360

actactacgt?cttttgctag?gcttgcattg?gcagtggcct?cagttggtat?tgtctttgct 420

agtccaacaa?aaaataacga?tgggaaactg?gtatatggct?caccagaatc?cgtcggcatg 480

atatccgccc?ctttgcacca?aatggtccaa?aatgttagcg?catatacaca?tgctgccaac 540

tatagcaagt?tctcgtacga?caaagtccat?cccatcgagc?cagggtctgt?taccctggtg 600

gctctcgacg?gtgtcatcgt?cagcgaattt?gccttgggca?agagaaatct?ctacgccgat 660

gtcaacggca?ccaatttacc?tcgatacctg?caggaagaca?ccaccctgga?tacagtctac 720

gatatggcaa?gcctcacgaa?gctgttcacc?acggtagctg?ctttacggga?acttgacgct 780

ggtcgaattg?cgcttaatgt?aactgttgca?acttatatac?cggactttgc?gacgaatggg 840

aaggagaata?ttactatctt?ggagctgttc?acgcatacaa?gcggtttcgc?ttctgatcca 900

tcgccaccac?ttttctctgc?ttattatacg?acgtatgatg?aacgcattaa?agcaattttg 960

acgcaaaaaa?ttatcaatac?ccccggcagc?acatacctct?acttagatct?caactttatg 1020

tcgctgggcc?tcgttatcga?gaccgtaacg?ggacgtgccc?tggatgatct?tatttatgac 1080

ttcaccagac?cgcttgaaat?gacatctacc?ttcttcaacc?gcgggaatat?cgaaggctct 1140

acaccccagt?cacccaacta?cgaccgcaca?gccgtacaag?aatttcagat?cgcagccctc 1200

ggaccctcag?aaccacagcg?tccacaacca?gtgcgcggca?cagttcacga?cgagaacgca 1260

tggtccctag?acggcgtatc?aggtcatgca?ggtctattct?ccactgtgcg?cgatacagcg 1320

acattctgcc?agatgatcct?caacaacggc?acatatgcag?gccaacggat?cctttctcga 1380

acagcggtag?acatgatttt?cacaaacttc?aatgccaggt?ttccggggga?tgctcgtagt 1440

ttagggtttg?agttggatca?gtattctact?gcgggaccga?tggcgagttt?gcaaactgcg 1500

agtcacactg?gatttactgg?gactacgttg?gtgatggata?ggacgtataa?cgccttttgg 1560

ttgcatttta?gtaaccgggt?gcatccgtct?agggcatggt?ctagcaatac?tattgtgaga 1620

gaggctattg?ggtattgggt?tgggaagagc?ttggggttgg?atgttgcgtt?tgctctgttg 1680

taatggttga?gcggaggacg?taccagatgg?gatcctggat?tcattccttc?gatacacttg 1740

tatgtacacg?aggaatgtat?tcgaatgcaa?atacctcata?tattacagaa?tccgcagtat 1800

gaatccggat?aattttgtta?aggcaaatcc?agaaatttct?aaaggttcac?taccataaac 1860

acaaatattc?accattcaat?atggcatgtt?atcatccatt?ctcataaaag?accctcgatc 1920

cggaagaccg?atcctgtcag?ggaattagtc?aaccctctcc?aataaatgca?gcatgaaaga 1980

aatctaccat?gaatcaaccc?cgagccctaa?ataatccgaa?aagatccagt?ctggtgacag 2040

tgcaggg 2047

<210>22

<211>2730

<212>DNA

＜213〉aspergillus niger

<400>22

tggccacgcc?atcctcttat?gctctctcta?tccactctct?ccctcagctt?ctctcaacca 60

cttccttacc?catctttcac?cctctcttcc?cctttccccc?tttcaaatct?cttgaccttc 120

aagtctggcg?cgcgtctgga?cagcggctct?tgtgctagag?gtcttaactc?ctaccccgcc 180

ttcctctggc?cttgaccttc?tctgttcctg?aaccaggttc?cctcccggtc?ctctccgccg 240

acgttatccc?cgacattgtg?ctgctcatta?cctccgcccc?caaggtaacg?ggtaagcgaa 300

aagtgcaagg?cccagttggc?taaatttcga?tgactccttg?agcgcgactc?caagtccgct 360

tgggaccgtt?tcctccgctc?cgtcttcggc?aatatctact?actactacta?ttactattac 420

taccccggtc?agccgccgcc?gccgtattac?aagtacccat?cgcctactga?tggtctcctt 480

atcattccgc?ggggagacca?cttccattcc?ctccatggcg?tcctggttgc?tctcgacgct 540

cctttttctg?agcccgtcct?tggtgtcagc?caaatcggcc?gcagactatt?atgttcactc 600

cttgcccggt?gcccccgagg?ggcccttgct?gaagatgcat?gccgggtaag?cttcgctgtc 660

ccggaacggt?ccgcttagca?cctatatact?gacttattgc?ctccacgcgc?agccatattg 720

aggtggatcc?acagaacaat?ggaaatcttt?tcttctggca?ctaccagaat?cgccatattg 780

ccaaccgcca?gcggactgtg?atctggttga?acggtggtcc?cggatgtagt?tccatggacg 840

gcgcgttgat?ggaggtcggt?ccgtatcgcc?tgaaggacaa?tgaaaccttg?acctataatg 900

agggttcctg?ggacgaattc?gccaatttgt?tgttcgtcga?tcagccagtc?ggaaccgggt 960

tcagttatgt?caacacggac?agctatcttc?atgagctcga?tgagatgtcg?gctcagttca 1020

ttgtctttct?ggaagagtgg?ttcagattat?ttccggagta?tgaacgcgat?gatgtatgct 1080

gcaatcacct?atgctgccct?agtcctgcac?cttcacggat?tctgttctaa?catctgcgac 1140

agatctacat?tgccggcgag?tcttacgccg?gtcagcatat?tccatacatc?gccaaagcca 1200

tccaggaacg?gaacaagaac?gttcaaggga?agaccatcgc?ttcgtggaat?ctaaaaggcc 1260

tattgattgg?caatggttgg?atttctccta?atgaacagta?catgtcctac?ttgccctacg 1320

catatgaaga?aggccttatc?aaggaaggca?gccggaccgc?gaaggaactc?gaagttttac 1380

agtcagtctg?taagtccagg?ctggaaactg?gcaagaacaa?ggtccacctc?aacgactgcg 1440

agaaggtcat?gaatgctctg?ttggataaga?cggtcgaaga?caacaaatgt?ctcaacatgt 1500

atgacatccg?ccttcgtgac?accaccgatg?catgcggtat?gaactggccc?accgacctgg 1560

aggacgtgaa?gccctatctg?cagcgggaag?atgtggttaa?agcgcttaac?atcaatccgg 1620

agaagaagtc?tggctgggtg?gagtgttcag?gtgcagtgag?cagcgctttc?aatccgcaaa 1680

agtccccgcc?ctcggttcaa?ctacttcccg?gcttgctgga?atcgggactt?caaatcctcc 1740

ttttcagcgg?agacaaggac?ctgatttgca?accatgttgg?aacggaacag?ctcatcaata 1800

acatgaagtg?gaacggaggc?acgggtttcg?agacctcacc?tggcgtctgg?gctcctcgac 1860

acgactggag?tttcgaaggc?gagccggcgg?gtatctatca?atatgccaga?aacctgactt 1920

acgtgctcat?ctacaacgca?agccatatgg?ttccctacga?ccttcctcgt?cagagccggg 1980

acatgctaga?tcgcttcatg?aatgtcgata?tcgcgagcat?cggaggcagc?cccgccgact 2040

cgcgcattga?cggcgagaag?ctgccccaga?cgtcggtggg?cggccatccc?aacagcaccg 2100

cggcggagga?gcaggagaag?gagaggatca?aggagacgga?atggaaagcc?tacgccaagt 2160

caggcgaagc?cgttctcctc?gtcgtcatta?tcggtgtatt?agtttggggc?ttcttcatct 2220

ggcgcagccg?ccggcgtcac?cagggatacc?ggggcgtctg?gcataaggac?atgagcggaa 2280

gctctgttct?cgagcggttc?cacaacaagc?gcacgggagg?cgcagacgtc?gaagcggggg 2340

atttcgacga?ggcggagctc?gatgaccttc?attctccaga?cctcgaaaga?gaacactacg 2400

ccgtgggcga?ggacagcgac?gaggatgata?tttcacgaca?gcattctcaa?caggcctccc 2460

gagccggggg?cagtcataat?ctatcctagt?tcatctttgg?ttgggtaaac?ttgtgatggt 2520

gtaggtgtat?ggcttgcttg?gggtctttgc?cttttgtttt?tgttttcttg?gccacgaaag 2580

gacgtgcctc?cttatatgtg?catttatcat?tttatctagc?tggctgcctg?gcatcattta 2640

acatttagac?atgaacaaag?tttatgaccg?cttcctgata?catagccagg?cggaaattcc 2700

tcccgtctat?aagccaacag?tctcatccac 2730

<210>23

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>23

actttattgc?caagcgaatg?acttcgactt?tcttatttct?gcaacgcgtt?tggccagtta 60

ataatttgct?agactcaact?gattgctcgg?tgccatctta?ccagggatcg?gcaagccgaa 120

aagtacgcta?ttgcacccaa?tctgtaaaga?agacttctcc?aaactccaaa?gtagaacaga 180

cccatcttta?gctttaataa?ctccaatcat?gacgattgga?actatcggaa?aatataatct 240

aagttcaatg?gatctggtcc?gtcgctagaa?tatcggagta?aatgagccca?acccctggcc 300

atcaggttcg?acggaggaat?tcttctggtt?gggtacaaga?ttgacacgat?ccccttggcc 360

gctgaacgtc?actcttccgc?tgtcaaagct?tgttggctga?cttcgatccc?ggatgatgtt 420

tctgatttca?cctgcagtga?cagttgcggc?tgcacttctg?ctgatcaacg?gcgcaggagc 480

aactcaatct?gaacgaagtc?gggctgccgc?tcatttttcc?aaacgtcatc?cgacgtaccg 540

tgctgcgacc?agagcccagt?cgagcaacac?ttccgactac?cgattcttca?ataataggac 600

caagcgtatg?tattacaccg?gccatgaact?ctattcgtcg?ctgacatgtg?gtcatccctg 660

gatagcccac?ttggtggaaa?gcttacccga?tgtgcacttc?gatgttgggg?agatgtactc 720

ggggtcgatc?cctatcgatg?acagcaacaa?tggatctcga?tccctgtttt?atatcttcca 780

acctaagata?ggcgaacctt?cagacgacct?taccatttac?ctcaatggag?ggccaggctg 840

ttcctccgaa?cagggattct?ttcaggaaaa?tggcaggttc?acatggcagc?ctggtaccta 900

tgcacccgtc?atcaacgaat?attcttgggt?caatttgacg?aacatgctat?ggtacgcttc 960

ttcctgagga?ttaaattgga?ggtatgcact?ctgtacattt?tatgagacta?atgtgaataa 1020

attcagggtt?gaccaaccag?tcggaaccgg?attttccgtt?ggaaatgtta?cagccaccaa 1080

cgaagaagag?attgccgccg?attttctcga?cttctttgaa?aagtttgaag?atctatacgg 1140

gataaagaac?tttcgcattt?tcatgaccgg?tgagagctac?gccggtcgct?atgttcccta 1200

tatctcgtcg?gcaatgctag?acaagaacga?caccacgcgt?ttcaatctga?gcggtacgag 1260

ccctgtctac?tcataactac?tacccctata?agcttagttg?actgaaactg?actttttaac 1320

gtccctctag?gagcccttct?ttatgacgcc?tgcatcggcc?aatgggacta?catccaggcc 1380

gaactccctg?cctacccctt?cgtcaagcag?cacgcttcac?tattcaactt?caatcagtcc 1440

tacatgaacg?agcttgaaac?cacctacgaa?gaatgcggct?acaaggccta?cttcgatgag 1500

tactttgcct?ttccaccaag?cggcatccaa?cccccaaaat?acatgaacta?ctccgagtgc 1560

gacatctata?acatgatcta?ctacgaagcc?tataacccga?acccatgctt?caatccctac 1620

cgcgtcattg?atgagtgtcc?acttctctgg?gacgtcctgg?gctggccgac?agacttggca 1680

tacgagcctg?cgcccaccac?atacttcaac?cgtatcgatg?tcaagaaggc?cctgcacgcc 1740

cccatggatg?tggaatggga?gctctgcagc?tacgacctcg?tcttcgctgg?aggcgacgct 1800

gacccgggtc?cggagcagca?aggggatgac?tcacccaacc?ccaccgaggg?tgtcctcccg 1860

cgtgttattg?aggcgaccaa?ccgcgtgctc?attgccaacg?gtgactggga?ctacctgatt 1920

atcaccaacg?gcaccctcct?cgccatccag?aatatgacct?ggaacggcca?gctgggcttc 1980

cagtccgcac?ctgccacacc?gatcgatatt?cagatgcccg?atctccagtg?ggttgagatt 2040

tttgaggccc?aggagggata?tggagggctg?gatggccctc?agggggttat?gggtgtacaa 2100

cattatgagc?gcggtttgat?gtgggcggag?acatatcagt?cggggcataa?gcaggctcag 2160

gatcagggcc?gtgtctcgta?tcgccatctg?cagtggctgt?tggggcaagt?tgagattctt 2220

tagtctcccg?ccttagatat?aattgatatc?acactgtaat?gttccctcca?aaaagcttca 2280

tttattgata?gtttcagtga?attttgttac?ttccagtaac?aactctatta?tggcattccc 2340

tggtttttaa?tgctgcctcc?ttttaccgta?gctacggcta?tgactacata?taacggactg 2400

atttttctat?ctcaacttaa?ctttgtattg?ccccctggat?cctgactaca?gaaaataaat 2460

aattcaaatt?gatatttatt?ggccttttat?tggcagcata?atatctttct?catataagca 2520

cgcgagaaac?ttgttcctca?tcatactata?tttatcccca?cagctgatgc?cgtgattcgg 2580

taatgaagat?ggagattatg?cggattgcgc?acttcataat?caaacatcgt?ataagtgtgt 2640

cagtaatata?tatctataat 2660

<210>24

<211>2800

<212>DNA

＜213〉aspergillus niger

<400>24

aatgccgaag?cttgacctga?tacgacttca?aggtatcgtc?accgacaatc?gttatcatca 60

cgctacaggc?ccgcagtttc?cgcttgaatt?cccgcattag?gaaatgagca?tcgcattcct 120

cttcccacga?ggtctctttc?cgagggcagc?cgctgcaaca?tcattgggat?catgcttggt 180

tctcctctcc?catagctgtc?cgcgagcttc?tcattggtac?ctcttcgcta?cctcgttgca 240

tcctattcgc?gcatggcccc?gccagagatg?tttctgcaag?gtcccatcac?cttgccgcgt 300

tgctattccc?cgccctcgag?ttcccgacaa?gttactttgt?gtcagtggct?gagaagcctg 360

gttctgagag?tgtactcaga?caatcatatg?gttccctcca?tgtgctacgt?cgtcctagcg 420

tcgctgcact?acatcatcgt?taggcagcat?ggaactggca?cccgcacata?aagcccccga 480

cacccccatc?gataggctcg?gtgttcgtgc?acgcctgtcc?actggcccct?cccccaaagg 540

cccttcatca?gtatgctgtt?tcgcagtctg?ttgtcgacgg?ctgtcctagc?cgtctcgctg 600

tgcacggata?atgcttcagc?tgctaaacat?ggtcgatttg?gccaaaaagc?tcgcgacgcc 660

atgaacatcg?cgaagcgttc?cgctaacgcc?gtgaaacact?cgttgaagat?ccctgtcgag 720

gactatcagt?tcttgaacaa?caagactaag?cgtatgtatc?tcagttcgat?attgaacgat 780

ggctgatttg?cttccgtcgg?acagcttacc?gcgtggaaag?cctgcctgat?gttcacttcg 840

atctgggcga?gatgtattcc?ggcttggtcc?ctattgagaa?gggcaacgtg?tcacggtccc 900

ttttctttgt?cttccagccc?actattggcg?agcctgtgga?tgagatcacc?atctggctga 960

atggtggccc?tggttgcagt?tcccttgagg?cctttctcca?ggagaatggt?agattcgtgt 1020

ggcagcctgg?aacctaccag?cctgttgaga?acccatactc?gtgggtgaat?ctcaccaatg 1080

ttctgtggta?agtgtgatat?tactggatcg?ctagttgagt?ttacatgggc?ggtatcgacc 1140

taacctattt?tttgtagggt?tgaccaacct?gtgggaacgg?gattctctct?gggtgtccca 1200

accgctacgt?ccgaggagga?gattgctgaa?gactttgtga?agttcttcaa?gaactggcag 1260

cagatctttg?ggatcaaaaa?cttcaagatc?tatgttactg?gagaaagtta?tgcgggccgt 1320

tatgttcctt?acatatccgc?tgctttccta?gatcagaatg?atacagaaca?cttcaaccta 1380

aaaggtgagt?tatacttcac?caaagtaatc?tttaactagg?gcttgtactg?attgtactat 1440

ctaggtgcac?tggcatatga?tccctgtatt?ggtcagtttg?actacgtgca?ggaggaagca 1500

cctgttgttc?cctttgtcca?gaagaacaat?gccctcttca?atttcaatgc?aagctttttg 1560

gcggaactag?agagcatcca?tgagcaatgt?ggatacaagg?atttcatcga?ccagtatcta 1620

gtcttcccag?catccggtgt?ccagccgcca?aaggctatga?actggagcga?tcccacctgt 1680

gatgtttatg?acatcgttaa?taacgccgtc?ctggatccca?acccgtgctt?caacccctac 1740

gaaatcaacg?agatgtgccc?cattctctgg?gacgttcttg?gattccccac?cgaagtcgac 1800

tatctccctg?cgggcgccag?catctacttt?gaccgcgctg?atgttaagcg?tgccatgcac 1860

gctcctaaca?tcacctggtc?cgagtgctcg?gtggagagcg?tctttgtcgg?gggcgacggc 1920

ggtcccgagc?aggagggcga?ctactcggcc?aaccccatcg?agcatgtctt?gccccaggtc 1980

atcgaaggca?ccaaccgagt?tctgatcggt?aacggtgatt?atgacatggt?catccttacc 2040

aacggcaccc?ttctctcgat?ccagaacatg?acatggaatg?gaaagcttgg?attcgacacg 2100

gcccccagca?cccccatcaa?catcgacatc?cctgacctga?tgtacaatga?agtgttcatt 2160

gagaacggct?atgacccaca?aggtggtcag?ggtgtcatgg?gcatccagca?ctatgagcgt 2220

ggtcttatgt?gggctgagac?cttccagagc?ggacacatgc?agccccaatt?ccaacccaga 2280

gtgtcatacc?gtcaccttga?gtggctgctt?ggccggcggg?ataccctgta?aggcgggtag 2340

gctaccacgg?gggacgatgt?cacgatgata?gtcataagtt?atgatctgta?gatacgttgt 2400

atgcgaatgt?acatgaattg?cttttactgg?cagtctctaa?agcaaaattc?atagtagagt 2460

actggcctac?ttaccctcac?ttcccctatc?ttttcaacct?gaagaccgga?agaattgtaa 2520

ctaacaagca?taacgtagct?gatttgaagc?agagcataac?acactctacc?cctcggcact 2580

tctacttatg?acgctatttg?actgctaact?cgggtttaat?cctgaagctg?cagtccaatc 2640

gtacattaaa?ctcaatgtgc?cttgcccagg?aaacgatatt?tgacttatat?gatctgaaaa 2700

tgaacaattg?tccccgagag?agagagagag?agcgagcggt?aaatacttag?caagtcagtc 2760

acgcagtatc?ctccactaat?gccgtaacac?aggaaatgga 2800

<210>25

<211>2165

<212>DNA

＜213〉aspergillus niger

<400>25

gaatcaggat?ggaggcccga?cggccatcaa?ccgccgaaca?tccggctgct?cgaccgaatt 60

cggagacatg?gtcggagcta?ggcgggaata?gccgtccaaa?gtcacgatag?gtcgcttgta 120

ctgtgtagaa?gtcagaccct?ttggatccag?cgttcgttgt?gaatggctcc?acgcagcctt 180

tgctttgacc?aatttggggc?tgagtcactt?ggtcgatgat?gactggctat?accagtgcaa 240

agtgtgagca?ccatgcttac?actattgcct?tagcctggcc?gttgaagctg?acatgagagg 300

aacaggcaga?agagaaatgc?tgccttgcct?cttgggggcc?aatttaacct?ccccagactg 360

cggcacatcc?gtcttggact?tgtgcttctt?tgcaacttac?gttataacga?gtctgtgaac 420

aggaaaacag?acgcccggtc?agtcaagatg?aaaggtgcgg?cgctaattcc?tcttgcggcg 480

ggcattcctt?ttgcccatgg?cctgtctctc?cataaacgcg?acgggcctgc?cgtcgttcgt 540

atgcccattg?agcgcaggag?cgcccagtcc?ttgcagaaac?gagattctac?ggtcggtgtg 600

actttgcaga?actgggtatg?ttaagctata?cggccgtgaa?gtatagatca?tgctgacaat 660

cgctaggatg?cgacctatta?cgcagtcaac?ctgacgttag?gaacacctgc?gcaaaaggta 720

tcattagctt?tggacactgg?cagcagcgac?ctctgggtga?acaccggcaa?ctcaacttac 780

tgctcaatcg?acaatctatg?caccccttat?ggcttgtaca?atgccagcga?atcgtctact 840

gtaaagaccg?tgggcacaca?cctcaacgat?acatatgcgg?acggcacaaa?cctttacggt 900

ccttatgtga?ccgataagct?cacgatcggc?aacacaacaa?tcgataatat?gcagtttggg 960

atcgccgagt?caacgactag?taaacgtggg?tgaaccgttt?gtccatgaat?gatcgtcgct 1020

gactaggtct?actataggcg?ggatcgccgg?cgtcggttac?aagatttcga?cctaccaagc 1080

cgagcatgac?gacaaagtct?acgccaacct?ccctcaggcc?ctcgtcgaca?gcggtgccat 1140

taagtctgct?gcgtacagca?tatggctaga?tagtttggag?gcgtcgactg?gctccctcct 1200

tttcggaggt?gtcaatacag?ccaagtacaa?gggcgatctg?cagactcttc?cgatcattcc 1260

tgtgtatggc?aaatactact?ccctcgccat?cgcccttacg?gagctcagcg?ttgcgaccga 1320

ctccaactcc?agtagcttca?ccgacagtct?ccccctctct?gtgtcactcg?atactggcac 1380

caccatgacg?gcactgccca?gcgacctggt?caacaaggtc?tacgatgcgc?tcaacgcaac 1440

ctacgacaag?acatacgaca?tggcctacat?cgactgcgac?actagagagg?cggattacaa 1500

tgtaacatac?agtttctccg?gggcaacgat?caccgtgagc?atgagtgagc?tgattatccc 1560

cgcaacggag?ccggggtggc?ccgacaacac?gtgtgtcttg?ggcctcgtgc?ctagccagcc 1620

gggcgtgaac?ctgctcggtg?atacattcct?gcgcagtgcg?tacgtcgtgt?atgatctcga 1680

gaacaacgaa?atctctctcg?ccaataccaa?tttcaatcca?ggcgacgatg?atatcctcga 1740

aatcggaacg?ggaacgtctg?ctgtgccagg?agccacaccg?gttccctctg?ctgtctcttc 1800

tgcaactgga?aatggactga?tctcgtctgg?caccgcagtg?cccacgctgt?cgggtgtcac 1860

aataactgct?acagccacag?caaccggctc?aaccggcact?ggctctagcg?gtggttcgtc 1920

ggctgaagcc?acgagtactt?cctcggaggg?cgctgcggcg?caagctacga?gcaacccgat 1980

gaacctgctc?ccaggacttg?cgggtatcgg?cctacttctc?gctctgtaac?gcgattgtac 2040

ctactcaaat?agatatcacg?acgagactct?aatgtaataa?tgtggtatac?aataacccca 2100

atatctacat?ttccttaacc?gtaaactgca?tactctacac?caaatccacc?accaaaatac 2160

atacc 2165

<210>26

<211>2800

<212>DNA

＜213〉aspergillus niger

<400>26

gcccagccaa?tcataagaaa?cccggtcctg?gcaaggtcttggcggggatg?gtgcaagcca 60

gccaaacaac?cggtttgttg?gacgccctgc?ggtacactga?aatcttgggc?tgttcgacaa 120

aaacaaagca?ggtaacacaa?agatataata?cggcggaatg?ataggatatc?cctgtcgatc 180

agccggacag?gatggcgcta?gtgtcacctc?cagcttcggc?taccctcgca?gcggacccaa 240

tcagcgtccc?tcccagcccc?cgatacagta?atggtatgca?catcgcagtc?ttaatcgcct 300

gcagcggcag?taatgatagt?cctgccggtg?aataataccc?cataacaaac?aaataaataa 360

tactacttat?ctctcctcgt?ccctttcact?ttccctttgc?cgtcttcaat?cccctcatct 420

tggtctcttc?ggcagccttt?caccatgctg?tcgtctctcc?ttagccaggg?agcagccgta 480

tccctcgcgg?tgttgtcgct?gctcccttcg?cctgtagccg?cggagatctt?cgaaaagcta 540

tccggcgtcc?ccaatggtga?gttatagacc?ccaattcttc?attttgagcc?acatactgac 600

gtgattcctt?cgaatactac?caggctggag?atacgccaac?aatcctcaag?gcaacgaggt 660

cattcgcttg?caaatcgccc?ttcagcagca?tgatgtcgct?ggtttcgaac?aagccgtgat 720

ggatatgtcc?acccccggac?acgccgacta?tggaaagcat?ttccgcaccc?acgatgagat 780

gaagcgcatg?ttgctcccca?gcgagactgc?cgtcgactca?gtccgcgact?ggctggaatc 840

cgccggtgtc?cacaatatcc?aggtcgacgc?cgactgggtc?aagttccata?ccaccgtaaa 900

caaggccaat?gccctgctgg?atgccgactt?caagtggtat?gtcagcgacg?ccaagcatat 960

tcgtcgtctg?cgcaccctgc?aatactccat?ccccgacgcc?ctggtctcgc?acatcaacat 1020

gatccagccc?accacccgct?ttggccagat?ccagcccaac?cgtgccacca?tgcgcagcaa 1080

gcccaagcac?gccgatgaga?cattcctcac?cgcagccacc?ctggcccaga?acacctccca 1140

ctgcgactcc?atcatcacac?cgcactgtct?gaagcagctg?tacaacatcg?gtgactacca 1200

ggccgatccc?aagtccggca?gcaagatcgg?ctttgccagc?taccttgagg?aatacgcccg 1260

gtatgccgat?ctcgagaggt?tcgagcagca?cctggctccc?aatgccatcg?gccagaactt 1320

cagcgtcgtc?caattcaacg?gcggcctcaa?cgatcagctt?tcatcgagtg?acagcggcga 1380

agccaacctc?gacctgcagt?acatcctggg?cgtcagcgct?cccgtcccca?tcaccgagta 1440

cagcaccggc?ggacgcggcg?aactagtccc?cgacctgagc?tcccccgacc?ccaacgacaa 1500

cagcaacgag?ccctaccttg?acttccttca?gggaatcctc?aagcttaaca?actccgacct 1560

cccacaagtc?atctctacct?cctacggtga?agacgaacag?gtatgcacct?cacctgaccc 1620

attccatttt?acatccctca?cctctctcaa?ccaaactaac?aacaccaaca?gactatcccc 1680

gtcccctacg?cccgcaccgt?ctgcaacctc?tacgcccaac?tcggcagccg?cggcgtctct 1740

gtaatcttct?ccagcggcga?ctccggcgtc?ggcgccgcct?gcctcaccaa?cgacggcacc 1800

aaccgcacgc?acttccctcc?tcaattcccc?gcctcctgcc?cctgggtaac?ctccgtcggc 1860

gcaacctcca?agacctcccc?cgagcaagcc?gtctccttct?cctccggcgg?cttctccgac 1920

ctctggcccc?gcccctccta?ccaacacgcc?gccgtgcaaa?cctacctcac?caagcacctg 1980

ggcaacaagt?tctcggggct?tttcaacgcc?tccggccgcg?ccttccccga?cgtctccgcg 2040

cagggcgtca?actacgctgt?ttacgacaag?ggcatgcttg?gccagttcga?cgggacgagt 2100

tgctccgcgc?cgacgttcag?tggcgtcatc?gcgttgttga?acgatgcgag?actgagggcc 2160

gggttgcctg?tgatggggtt?cttgaatccg?ttcctgtatg?gtgtcggaag?tgagaagggt 2220

gcgttgaatg?atattgtgaa?cggcgggagt?gtgggttgtg?atgggaggaa?tcggttcggg 2280

ggcacgccta?atggtagtcc?tgttgtgccg?tttgctagtt?ggaatgccac?gaccgggtgg 2340

gatcctgtgt?cggggttggg?aacgccggat?tttgcgaagt?tgaaaggggt?ggcgttgggt 2400

gaggagggtg?gtaattaagt?gtgagatggg?gggaaaggga?ttttcttttc?gatgtgaata 2460

ttaggtgaat?tgtgtggata?attttcatac?ataattaagt?ctgcattggc?agtgataacc 2520

tggaagaaat?gtctaatgag?tgtgatttgt?ttacttatgt?atattgagta?atggaatgta 2580

gatgacttgt?ctttgtactg?tataacgaaa?tgattatttg?agtggagggt?attaaagaac 2640

tataaaatat?atacaaaggt?taacccatgc?agtcgtaacc?cataatgcaa?agctctactc 2700

tatctgtatc?ggtagcagat?aagtgtatgc?aatctatctt?tgttgatgat?gcaatcaagc 2760

gggcacacca?ccagtgccaa?acagctccat?cttgatgcgg 2800

<210>27

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>27

ggagacagaa?tacatggaat?tgatgctcaa?caacgacaag?gagctcgcgt?tccgtaatgc 60

caagttcatc?gcttagattg?ggaggatcgg?ccaggtctgt?atataggccg?cgatagaaca 120

cgaaataaat?gatttatgac?tatttgcggg?catgagccct?cacgtagtgt?gctatagacc 180

gtagacttgg?taaacattgc?tatcgatccc?ccccttcgta?tattagtcat?aattcttcct 240

gatgtcaact?ctcaaaacac?agcattccac?agcgttcata?tgatgtcaat?gtctcaatcc 300

ctaacgtttt?gctgaaacag?gtacctcttg?gttatttttc?atatagtagg?aaggtcgccc 360

ttgcatgtgg?tatgagacca?ttggcggcat?ctagcttgaa?tggtactcaa?tatgaccagt 420

agtctccact?tcagcagtgt?gtttgtgata?gccaagacat?cggcagtagt?tcctgcagag 480

cttctacacc?tcttcatctt?cacttcacca?atccactctc?tcactgctca?cttctagttt 540

cgcaacacta?gagaatcatg?tggctctttc?tcgtgtgcag?tatcctgctg?ccacttggag 600

tagtcaacgc?acagtctcaa?tacttcaaca?acaaaaccaa?aggtatacca?catcgcgtca 660

gtttgaatcc?cactaacagc?gctagaattc?gtcgtcaatg?gctctgctat?tccttttgtc 720

gatttcgaca?ttggcgagtc?ctatgcgggc?tacctaccca?acacgccttc?tggaatctcg 780

agtctatact?tctggttctt?tccatcttct?gatcctgatg?cgtctgatga?ggtatgctta 840

tcgccgtctc?atatgcttcg?cacggctaat?aaacagatca?ccgtctggct?gaatggcggc 900

ccaggatgca?gctctctggc?aggcatcatg?ctcgagaacg?gcccctttct?atggcaacct 960

ggtacctacc?gacccgtgcg?caacccttat?gcctggaaca?acctcacaaa?tatggtgtac 1020

attgatcagc?ctgctggaac?gggattctcg?cttggcccgt?ctacggtggt?ctcagaattt 1080

gatgtagcca?gacagtttat?ggacttctgg?aggcggttca?tgaaaacatt?cgatctgcag 1140

aatcgaaaga?tatatctcac?tggcgagagc?tatgcgggcc?agtacatccc?atacatcgcg 1200

tcgcagatgc?ttgaccagga?tgatgatgag?tatttccggg?ttgccggcat?ccagatcaat 1260

gatccctaca?tcaatgagct?gccagttttg?caagatggta?tgcctctgac?acaggttatt 1320

gcttactcct?ctgacattga?tgacttccag?tgcctgcagt?tgcgaccgtc?aatcagcacc 1380

gctccctctt?tccctttaat?gacaccttca?tgagtcaaat?caccaagctt?tccgacgatt 1440

gtggctacac?ttcgtttctt?gacgatgccc?ttacctttcc?accccgttct?caattcccat 1500

cagtgcccta?taatgctagc?tgcaacatct?gggatatcat?aaacaacgct?tctctagctc 1560

tcaacccatg?cttcaaccgc?taccatatcc?ccgacgcctg?ccccaccccc?tggaacccag 1620

tcggcggccc?catcgttgga?cttggtccga?ccaactactt?caaccgcagt?gacgtccaga 1680

aagccatcaa?cgcgtaccca?acggactatt?tcgtctgcaa?ggatggaatc?ttcccgacgg 1740

ccaacggact?ggacacatcc?cctccaagct?ccctgggacc?gctgccgcgc?gtcatcgaac 1800

agaccaacaa?taccatcatt?gcgcacggcc?tgatggattt?cgagctgctg?gcgcagggaa 1860

ccctgatcag?tatccagaat?atgacctgga?atgggaagca?ggggttcgag?cgggagccgg 1920

tggagccgtt?gttcgtgccg?tatggtggat?catcgggagg?aggcgtgctg?ggaacggcac 1980

atacagagcg?tggattgaca?ttttcgacag?tatttagttc?aggacatggt?aggtccatat 2040

ctcagtatgc?gatgcagcgg?gttgctaaca?ggaatagaaa?tcccggaata?tgcaccgggg 2100

gcggcatatc?gccagctgga?gtttttgctg?gggagggttg?cgaatctgtc?ggtgggttga 2160

ccggagacag?caaagagaat?gaagaaaaga?aaaaaaaaaa?aaaaaaacaa?tatgaataat 2220

tgcagacaat?tattgagcag?gtgcagataa?cagagcagaa?tggttgagct?gtcagatcgg 2280

ttgacttttc?ggcaatgaga?tttggggaag?ctagctctga?gattggtcct?ccgccaaagc 2340

tatcgcaatc?atgatccaat?caacactgca?tactaccttg?atcccctcgc?tgtagacccg 2400

tattgccgga?caaacaattg?actagatatg?aactcttgat?tataaaccaa?tcaagacccc 2460

cagtagcgca?tattatcacc?cccatacgtt?catgatcggc?agcctaatcc?gaggtcggat 2520

cgggaattcc?ttcaaactct?gaaaccgcgc?tgggtccacc?ccgcatcttc?acagtaaaaa 2580

tctaagattc?ccctcaatcc?ctgctccatc?tactttcctc?cctctcatac?cacttttgct 2640

ttttgtatag?taaatatcca 2660

<210>28

<211>1540

<212>DNA

＜213〉aspergillus niger

<400>28

taaacagaac?taagcaaaag?tccagatctt?gtgtcccctt?cgactaccaa?tcggaacaat 60

aggaattaac?tattagcctg?caactgtctg?caccatcatg?caacccctct?cgttgtgatt 120

gctcctgttt?tacattcccc?gagacatgct?catctagttg?caagccatct?ctaacccacc 180

tgcatgaagg?gtgcagaaac?acgaacagtg?gcgtggtagc?acacactctc?tgcagtactc 240

gatgccaata?tttccaccag?ctgaactaca?atctccgcgt?cagaattaat?atataaccat 300

caccaatacc?ccttacatcc?caactcagca?actacagcaa?tacttacacc?tatcctccac 360

aaccccacaa?ctccacccca?tcatgtccaa?actctccgct?gctatctcca?aggtacttcc 420

ccatatccac?aacacccccc?acaccatccc?tcaaatctaa?caacaataac?aataacctct 480

caccacccca?gctctccctc?tccaccatag?ccaccactct?gctcctcctt?acccccccaa 540

ccaccgccta?cttctacaaa?tatcccgccc?tcttcgtcta?caaagacacc?aactgcaccg 600

atatctcctt?ctcacttgtc?tacccctccc?tgggtaactg?caacggcgga?tactacgact 660

acgcgggctc?attccagatg?ttcaatatcg?atgctgcgta?tacctgtaat?ggcagtgact 720

cgacactgat?gtttgagatg?tataatagct?ccggctcgga?ttgtggagat?gagagtgatt 780

tgttgtttag?acagccggtg?acggaggagt?gtactgttgc?ggatgtggag?agtccggggc 840

cgttggagat?gccggtttgg?tttgagttgg?ggtgtatgta?attgattcgt?tgagtcggta 900

aggagggatg?gacggggggt?tatataggta?gcttggtggg?taagcactat?tggggaattg 960

tggtgggatg?gctggtacta?tgttgttcgg?tgtggggatt?cttgagggtg?ggttagagac 1020

taaagtgagt?tgtctgaggc?agtagtggat?gacttctgct?tctgtgatgc?tggtatgtga 1080

taaagttaat?tacatatgca?aatgtgcaat?catagactga?taggtggaaa?cttgtctact 1140

cgttgagata?tatagttata?ctggaaatgt?tattcatcaa?ggctgaatac?aagtgtaacc 1200

gtgcacagat?tatctttgat?actgtctatg?ggctgtacga?gcgtctctga?ctcctacaat 1260

gagttagcgg?ctgcacatta?ctatgaggac?ctgtgaattc?gacaccaaaa?ataaccagcc 1320

ctgcagccga?ttacatctgc?ctttccatct?attttctgga?gtcaagcagg?tattccaaca 1380

tactcttact?tgcttaatac?accaactttc?atatatctac?ccagaattac?tcaatcgtga 1440

tgtaggcact?ggcaatcata?gtaactatat?ttcgtttcat?tttatttgtt?tgtacaattt 1500

tttttttccg?gtagtttaat?cattccaatc?aaagaattgt 1540

<210>29

<211>2800

<212>DNA

＜213〉aspergillus niger

<400>29

acactttctt?cgatgctatg?tcccagccat?ataaggttca?attctccgac?tcaagtgaca 60

gaatcagcta?tacacggttc?actgcttctt?atatagtaca?tgaggattca?ataaagccaa 120

gttcagcaga?gtcttggttc?cagagcttga?atactcggta?gcattccagg?gattgtgggg 180

agacaatcca?ggagcagcag?ccatcatttg?gtgcctgagg?cacacagcac?tgcctcgtct 240

ccactcccaa?tacttaccaa?gtcacccacc?attccttccc?tctttcggcc?tttctctttc 300

tctctctcac?ttctgctctc?gtgcaccact?tttcttccca?ctcacgccat?ccttctccat 360

ccatttcctg?ccaaactttt?gtctgtcatt?ttaggttttt?tgaaggagat?gactcggtga 420

ctactcttca?atcccggcgt?actcaacatc?cataatgcgt?cacctcttat?cactgctggt 480

gcttctgatc?gcatcggccg?ccctggtctc?cgccgtcccc?gccggctcca?ttatcactcc 540

acaaccaccc?gtcgagcccg?ttcaccttct?ctcttcccag?ccctctgatc?cccgaaggcc 600

atggatccgc?ctccgtgact?ggatcatcga?gtccatatgg?ggcatcgaaa?aacccgcatc 660

tcgtcgattc?ccactcaacg?attccccgcg?caatcgctct?cctccctccc?ggattctggc 720

gcgctacggt?agtgacgtcg?tacttcgttt?cagcctgcgc?aatcacgatg?aggccgaggc 780

attggcccag?gctgcagaca?ttctattcct?ggacgtatgg?gcgtctactc?cagcattcgt 840

agatatccga?ctggccgagg?aagtcgtaag?tggttcatcc?ttccgtccat?atctgcccgg 900

ttgactgatg?cctgatccac?tgtgaccctc?gcaacagatt?ccctcattat?tgggcttgct 960

accaaattcc?ctccagaccg?catatactcc?cctaatagac?aacctggcag?agagaatcta 1020

tacgacctat?ccatctaaaa?agccgatagg?acttgaagga?caatctggat?ttgcgtcctc 1080

gagtcgacct?gcgccaaagt?tcggtgacct?ttttttccac?gagtatcagc?ctttgtccgt 1140

cattatcccc?tggatgcggc?tgctggcttc?catgtttcca?tcccatgtgc?gcatgattag 1200

cgttggagta?tcttacgagg?gtcgcgaaat?tcccgccctc?cgactgagcg?caggcagctc 1260

caccgcggcg?tcaggccctc?gtaaaacaat?catcgttacg?ggtggtagcc?atgcccgcga 1320

atggattggc?acctcaaccg?tgaaccatgt?aatgtacacg?ctcattacca?agtatggcaa 1380

atccaaggcc?gttacccgcc?ttctacagga?cttcgactgg?atcatgatcc?ccacgatcaa 1440

tcccgacggc?tatgtttata?cctgggagac?ggaccgacta?tggcgcaaga?atcgacagcg 1500

gaccagccta?cgcttctgtc?ccggaatcga?tcttgaccgc?gcctggggct?tcgaatggga 1560

cggcggtcgg?acccgcgcta?acccttgttc?agaaaactat?gctggagacg?agcccttcga 1620

gggaatggaa?gcacaacaat?tagcacagtg?ggcgctcaac?gagacacaaa?acaacaatgc 1680

cgacatcgtg?agcttccttg?accttcactc?ttactctcaa?acaattctct?accccttctc 1740

ctactcctgc?tcctcgatcc?ctccaacgct?cgagagcctg?gaagagctag?gccttggcct 1800

agccaaggcc?attcggtacg?cgactcacga?aatctacgat?gtcacttctg?cctgcgaagg 1860

catcgtcacg?gccagtgcgg?cagataacaa?ccccgggcgg?ttcttcccca?ttggtggcaa 1920

ctccggtggc?agtgcgttgg?actggtttta?ccaccaagtg?cacgcgactt?attcatacca 1980

gatcaagctt?cgtgatcgcg?gaagctacgg?gttcctcctt?ccgtctgaac?acatcatccc 2040

caccggcaag?gagatctaca?atgttgttct?gaaattggga?tccttcctca?tcggaggcga 2100

ctcatttgac?gtcgattggg?aatcagaact?cttcgatctg?tcaaaggacg?aatccgatct 2160

ggatagccgc?tattcaaaat?ccaatgaccg?ctccccggcg?tatctacaca?acgccaacgg 2220

ccccctgccc?aacattgacg?aagacgaaga?taaggaatgg?gtaatggtgg?aggaagaaga 2280

ctacacagac?gatgacgacg?acgatgatga?tgatgatgaa?gaagaggaag?aggaagagga 2340

agatacatat?tgggccaccg?aacacacata?cgaatttcgg?cgacgacgct?gatgatggac 2400

aaactaatca?accctattta?tatgacaccc?tcgcccatat?actctccttc?tgatgacgat 2460

caataatgac?taatgacggg?tttgcgggag?tgattgacgt?gtttatcatg?ctctgcttct 2520

cgattctttt?tattatttta?gatactgttc?ccttcaggtg?cctatagcct?atagagctta 2580

ggggtattct?ctacataatt?aaatacagag?taattcaatg?aatcgtccta?tcaaataatg 2640

gacccgagta?actaactggt?gtttcattcc?ttccgtactg?tacttaatct?gtaaggaatt 2700

gaaagctaag?cctcataagc?tacttcaccc?cactctttac?ccaggtgcag?tgtatcatga 2760

tcatcgtcat?acattcattc?gttaggtatc?tttccgatgc 2800

<210>30

<211>2380

<212>DNA

＜213〉aspergillus niger

<400>30

cccgcctgta?caaggctcat?tgagcgacct?ttatttctat?gaaggcttct?tgcagtgtag 60

agccgctgtt?tagaactcgg?aaataggcgt?gcatagtatg?gacccaatca?acagagttaa 120

tctatttact?ctaacgccta?gcaagcaatc?agtgcccaga?ggaagctaac?ggatggctgg 180

ccaagctgcg?ccagaaacga?aatgagtccg?taataccatc?cctgcatgct?tatctgtatt 240

ctgtgcatgc?atgatgcttt?cctcatgggg?cattacccag?tagtccgaag?acgcaatgtg 300

accatctgac?tgagttttaa?atatactgtc?caagtgcctt?ctgacccggt?ccccgcttga 360

tggcaatcaa?caaaaggtga?atgtgactga?aaggcgcggt?ccagacaaca?ggacttagac 420

tttgttgtga?gactataaaa?ggatctaact?attgcactac?tgaaatcaag?tattctagtc 480

taccattgac?atttctcccc?tttcggtggc?tactcgctca?acatggcttt?cctcaaacgc 540

attctcccgc?tgctggccct?catcttacct?gcagttttca?gtgccacaga?acaggtccct 600

catccgacca?tccagaccat?cccggggaag?tacattgtta?ctttcaagtc?cggcattgac 660

aatgcgaaga?ttgagtctca?tgccgcatgg?gtaacggagc?tccacaggcg?cagcttagaa 720

ggccgcagta?caaccgaaga?tgaccttccc?gccgggatcg?aaagaacgta?cagaattgcc 780

aattttgctg?ggtacgcggg?gtctttcgat?gagaaaacta?tcgaggagat?ccgcaaacat 840

gaccatgttt?gtgtccacgt?atcctagacc?gtatggtttc?gactaattgc?tgtacaggta 900

gcttatgtgg?aacaagatca?ggtctggtat?ctcgatacgc?tagttaccga?aaggcgagct 960

ccttggggac?tggggagcat?ttctcaccgt?ggtgggtcta?gcaccgacta?catctatgat 1020

gacagcgctg?gggagggtac?atacgcttat?gtagtggaca?ccggcatctt?ggctacgcat 1080

aatgagtttg?gtggtcgtgc?tagcctggca?tataatgctg?cagggggtga?gcacgttgat 1140

gatgttggac?atggtacaca?tgtagcaggg?accatcgggg?gcaaaacata?cggggtttcg 1200

aaaaacgctc?acctactgtc?cgtgaaggtg?tttgtaggtg?aatccagctc?gacatcggtc 1260

attctggatg?gcttcaattg?ggccgccaat?gatattgtga?gcaagaaccg?gaccagtaag 1320

gcggcgataa?atatgagtct?tggtatgtgc?gccctctctg?gggatctaat?cccgctaacc 1380

gtgatgcagg?tggaggctac?tcctatgcgt?ttaacaatgc?agttgagaat?gcttttgacg 1440

agggtgtgct?ctcttgtgtt?gccgctggaa?atgagaatgt?aagctctgct?gaactgtcca 1500

ccattgagct?aaatttagac?taatgttttg?cagagagatg?cagcacggac?tagcccggct 1560

tctgcacccg?acgccattac?tgttgccgct?atcaacagaa?gcaatgcccg?tgcgtcattc 1620

tcaaactacg?gctctgtggt?tgacattttt?gccccgggag?agcaagtact?ttctgcatgg 1680

accggctcga?actcggccac?caacacgatc?tccggcacgt?ccatggctac?accccatgtg 1740

acaggtttga?tcctctattt?gatgggcttg?cgggaccttg?ctaccccagc?ggctgcaacg 1800

accgagctca?agaggttggc?tacgcggaat?gctgtcacca?atgtggcggg?tagccccaat 1860

cttctggcct?acaatggaaa?cagcggcgtg?tcaaaagggg?gtagcgatga?tggagatgag 1920

gactaggtgc?gtaacatgag?tgaatatggc?ttagaatagt?ggggatcgga?gagtagacta 1980

gtttatatgc?gaaataaagt?gtgtatcagc?accctggcct?gttcatgtaa?gtcggcattt 2040

tcacttttgc?cgacaccgca?aatatgctgt?gcttgaggct?gttgcctccc?cagccagcct 2100

tcccgagact?gaaactcaca?catccattgg?atgtataaag?ttctgcacat?gcgaaatgcc 2160

gctgccgttt?acctcccgac?gtggtaccgg?accgaaggca?gacacagatc?atggaccgct 2220

ataccgcaca?gacaacttgt?gctccttact?gaaagtacca?ttccacaggt?cattgcagca 2280

tgatgagtga?tgatgtactt?ctccccatca?agaaccactg?gcggtggttg?gaatgaatct 2340

agatcaaaga?gatcaaccgc?ttccccggac?agatcaggcc 2380

<210>31

<211>2441

<212>DNA

＜213〉aspergillus niger

<400>31

aaacgacgtt?ttaggtcaat?actgaagtcg?ttgaaaacgc?ttgattcttc?gctatctagg 60

cgcgctcgga?ggagcagttg?aagttacgga?gttcgggtca?cgtgacctcg?actactagta 120

atctactgag?attacgttcc?aacataattt?catcaggaag?aatgcaaagg?ccccagagaa 180

ggagaatttc?acgatcggtg?aaaacgacca?agcagcatca?catttgaatg?aaactacatt 240

gctccgtgtt?tattccgttt?ctctctcttc?tctctatcta?ttgcttctct?ttggctagac 300

ttcaccaact?aattaatgct?tacacgatga?tagctgcatc?cattgaatca?aactgacccc 360

gcaaagctga?ctgaaccaac?ccctccggtg?ctgttgctac?tgccagtttg?aattcaatat 420

cctataaccc?accctgctca?atgatcaccc?ttttgtcggc?cctgttcggc?agcgtagtat 480

atgccgctac?gcagaccgtg?ttagggccag?agggggctga?tccctttacg?gtgtttcgca 540

gcccacactc?accggcattt?tcaattcgca?tccaggagca?gaatgactcg?atctgtgatg 600

ctcgttcacc?ccaattcact?ggttggctcg?acattggccc?gaagcatctt?ttcttttggt 660

attttgaaag?ccagaatgac?cccttccatg?atcccctaac?gctatggatg?actgggggcc 720

caggagactc?gagtatgatt?ggacttttcg?aagaagttgg?cccttgccgg?attaatgagt 780

ttgggaatgg?aacagatcac?aacccctggg?cctggaccaa?gaattcatca?cttctttttg 840

ttgaccagcc?agtcgatgtc?gggttttcct?atatcgatga?gggctatgag?ctgcctcatg 900

actcacgtga?agccgcggtg?gacatgcatc?ggttcttgcg?attattcata?tccgagattt 960

ttcctcacaa?acagttcctt?cccgttcacc?tttccggtga?atcttacgca?gtaagataga 1020

agccgccccc?agaaaaagct?tgctcagtgt?tctaacaatt?accttgtgat?acactgtgta 1080

gggccggtac?attccttatc?tggcgaccca?aatcttggaa?caaaatgaac?tgtataaaga 1140

tagccccagg?ataccgctga?aatcgtgctt?ggtgggtaac?ggattcatgt?cacccaagga 1200

tgcaacgttc?gggtattggg?aaacactgtg?tactactaac?tcaggagtcc?catctcctat 1260

cttcaatgaa?actaggtgcg?atattatggc?ggcgaatatg?ccgcactgta?tggatctata 1320

tgacatatgc?attcaacact?cagaccccgc?gatatgtcat?gcggcccagt?ccgtctgtta 1380

cgatagtgtt?gtagggtagt?atgataacga?ggccggcgct?gatggccgta?acagatttga 1440

tagtgagttg?ttatcatcag?aggctcatgg?ccaaattatt?gctaaggatg?acgacagtca 1500

ctgcaccttg?tgagatcgac?gaaatgtgct?atatcgaagc?ggctctaatt?gagagatatt 1560

tgaattcgcc?atctgtttgg?gaggccctgt?cgccaccgca?acaggttacc?gaatacaaat 1620

tcgtcgctac?ttctgttatt?gatgcatttg?ctcaatcagc?ggacggcatg?gtgtcgagct 1680

cgaagcagat?cgctttctta?ctcgcaaata?atgttgactt?cttagcgtat?caaggcaacc 1740

ttgatctcgc?ctgtaatacg?gctggcaacc?tacgttgggc?gaactcgctt?tcttggaaag 1800

gccagacaga?atttaccgca?aagcccttac?ttccgtgggt?ctcgatcaac?tctgggagcc 1860

aggaacctgt?ggggagtgcg?aaggaaattc?aggtttcggt?cggtgaaggg?acggacgaaa 1920

cgtcacgctt?tgcctttgtg?actgtggaca?acgctggaca?cctggtaagt?caaagtaaaa 1980

ttaggagaga?ggggtttaat?gatccagttg?gctaacgagt?agtgttggta?ctatggtagt 2040

tgccccaaga?tcggccggat?gtagcgcttg?acatgatgat?tcgctggatt?actggggcat 2100

cctttgttta?acaaggaaca?gttgcgggac?tcaaagattt?caaactgacc?tactccttac 2160

cttttatgaa?atgggcccgc?tgcctaggtc?aaacaagggt?gctcgggcaa?acaataccag 2220

ttttggacta?ggggtccgtc?tgctttcatc?agcccttcgt?agcttggact?tttgtccatg 2280

tccctgctgc?ctcggtccat?cacacgcccg?ctccccccat?ttcctttcct?tcccttctct 2340

tttcgttcta?ttatgcttga?gtgcccgatc?ttccctgcat?gctatgccta?tttcagtgta 2400

cgcagtttgg?gtttttgtcc?gaggaagaca?atcatccatg?c 2441

<210>32

<211>3500

<212>DNA

＜213〉aspergillus niger

<400>32

gtttgttgtg?gttgttgttt?ggggtcccat?ttgcaatccc?tgaaccggac?cctgtcaatc 60

cctcccaagt?ccctcgttat?cttccgcctt?ttccaccccc?tccacacttt?atttttccat 120

ccatcatctt?ccagtgcctc?aatccaaatc?actgtcgttg?aatatagatt?cgcatctgtt 180

ttccgtacgt?gtatttgttt?actttcggga?gggagattct?cgccgttcat?tccagatttc 240

cgcccgtccg?ccaaggcggc?tgcgcctcca?gccaagccaa?aacctccttg?gatcgccagg 300

tggaaagaat?ccctttcact?cccccgtcga?gtgagtctgt?ctcactgctc?cctccggtcg 360

ttgcgcgcgc?tctctctttc?ctctccacac?ctccagtata?cttcctctgc?gggtgtgtga 420

gagactgcga?cgatccggag?aagaataaaa?ccctccagtg?cagcttaatc?tccggagtga 480

tccgtcatac?atgaccggct?cccgagccaa?gatctcgact?gggtccatcc?agtgaaaggg 540

aactcaatcg?tttgcacttt?tcgtcgctgc?ctgatgctgt?ggaccgaagg?gctctgttga 600

accctgactc?tccggatctc?tcggtgttgt?tttgatgcca?gcacgctcgt?tccacactca 660

cctttaacct?ctcccatcgg?ctgtcttcac?tcgctgacag?caatgcggtt?tctcacttat 720

tccctgccct?tcattgcaag?tgctatctcg?ctctttgggg?tcaatgtaca?aggttagctc 780

ggcgacatcc?ggcgcataga?tttgttctaa?ctggatcaaa?tacagctcga?tcacaagctc 840

caagtgccat?ccgtcatgtg?tcgacgcttg?accagcccac?catcaagaca?ccctcacagc 900

gggtcgatca?ccttgaccac?tttgacatca?ccttcaatat?tcatgacaag?caccagcgga 960

taaagctgga?gctggagccc?aaccatgaca?tcctggcgga?agacgcatcc?gtacagtatc 1020

tcgacgcgga?cgggaacgtg?cgacggcacg?agcccattgc?tccacatgag?cataaggtct 1080

tcaaggggag?gagtctactc?gggcgaggaa?aaggcatgtg?ggatccggtc?ggatgggcgc 1140

ggatctactt?gaagcaggat?ggctcagagc?cactatttga?gggagtcttc?agtatcgacg 1200

gcgacaacca?tcacgttcag?ctgaaatcgg?catacatgga?gaagaaacgc?cccgtggatg 1260

tcgaccttcc?cgactcagcg?actgactata?tgatcttcta?ccgggattcg?gatatggtgc 1320

gtctacatac?ggaactcaag?cggtcgtcgc?tcggatcgac?ctcgtgtcaa?gccgatcagc 1380

tcggcttcaa?cactaacccc?aaccaccctg?tgctacaacc?gtatggccag?gcagagaccg 1440

atacgtgggg?agcaatttca?ttgaactcct?tgtttggact?caacaagcgc?caatccgata 1500

tcggaagtgt?gtctggcaat?gcgggcggag?tcaatctggc?gtcgaccatt?ggtgatactt 1560

cgggctgtcc?gagtacgaag?caagtagctt?tgattggtgt?tgcaacggac?tgcgccttta 1620

ccggctcatt?caacaacgag?actgccgcca?aggaatgggt?catcagtact?gtcaacagcg 1680

cgtccaatgt?ctacgaaaag?tccttcaaca?ttacgattgg?gctgcggaat?ctgactatca 1740

ccgacagctc?atgccccgac?aacccgcccg?cggccacggc?atggaacatg?ccctgctcca 1800

gcggcaatct?cacctcccga?ctggatctgt?tttccaagtg?gcgcggtgag?caatcggatg 1860

acaatgctta?ttggaccctg?atgagcgatt?gcgcgacggg?caacgaggtc?ggactgtcat 1920

ggcttggcca?actctgcaat?agcgatgctt?cttcggatgg?ctcgagcacg?gtcagtggaa 1980

ctaacgtcgt?cgttcggtct?tccggctcgg?attggcagat?ctttgctcat?gaatctggcc 2040

acacctttgg?cgctgtccac?gactgtgact?cccagacctg?cgcggaggat?ctcgaagcct 2100

cgtcccagtg?ctgtccgttg?acctcgagca?cctgcaacgc?caacgggaaa?tacatcatga 2160

atcctacaac?tggaacagac?atcactgcgt?tctcgcaatg?cactatcgga?aatatatgcg 2220

cagccctggg?ccgcaacagc?gttaagtcca?gttgtctctc?cgccaaccgc?gacgtcacca 2280

cctacactgg?cagccagtgc?ggcaacggaa?ttgtcgagtc?cggcgaagac?tgcgattgtg 2340

gcggggaaga?tggttgcggc?gacaacaact?gctgcgacgc?gaagacatgc?aagttcaagt 2400

cgggagctgt?gtgtgatgac?tccaacgaca?gctgctgttc?aagctgccaa?ttctcctcag 2460

ctgggacggt?atgtcgtgcc?agtcgcggcg?actgcgacgt?ggcagagacc?tgcagcggca 2520

actccagtac?ttgtcctacc?gactcgttca?agaaggacgg?cacgagctgc?ggcagcagtg 2580

gctcgggact?tgcctgcgct?agtggccaat?gcaccagccg?cgactaccag?tgccgcagtg 2640

tgatgggcag?tctcctccac?agcaacgaca?cctacgcctg?ttcctccttc?agttcctcct 2700

gcgaactggt?ctgcacctcc?ccgaagatcg?gcacgtgcta?cagcgtcaac?caaaacttcc 2760

tcgacggcac?tccctgcggt?agtggcggct?actgcagcaa?cggcgactgc?aagggccaaa 2820

acgtcgaatc?ctggatcaag?aaccacaaag?gtatcgtcat?tggtgtcgcc?tgcgccgtag 2880

gcgccctgat?ccttttggcc?ctgatgacct?gcatcgtaaa?ccgctgtcgc?cgggctcgcg 2940

cgccaaaacc?cgtcccgcgt?ccagtgcctt?acgggccgtg?gcccggcgct?aggcctcccc 3000

cgccgccgcc?catgaaccag?tggccggcgc?gaggctatca?aggcttaggg?aatgagccgc 3060

cgcccccgta?tccaggtgta?cctggtcagc?cagtaccgca?acatatgcct?ccccaggggc 3120

ggtacgcttg?attgacgaga?cttcttctgt?gcttctttcc?atgcgatata?gtatgcatta 3180

cgattcttgc?agcactagca?ttacaaatgt?gacgattact?tcattgatcc?tttattgacc 3240

ttacattctt?gtcttggagt?ggaggcgtgt?tctagagttt?tggtttgatg?ctgaattgtt 3300

cttttctata?cgggggtctg?ggcgtttcgt?gaattgcagt?acttacaatc?caggggtgca 3360

atccccaggg?ggttggttcg?cgtcttcttt?ccctttcttt?gcccatatta?gacggggccg 3420

tgggatgtat?ggtcggggtc?tgatgtatgt?attcctgccg?tgtataatga?acttttccgt 3480

cataatatat?tgcttcttgt 3500

<210>33

<211>2520

<212>DNA

＜213〉aspergillus niger

<400>33

ttgatgcata?atgctttcct?tcagaggtaa?tacttcgagg?acggctatga?agcgtatgcc 60

acatacgatt?ggtatttgac?gatatttgtc?atgctataca?tgaaatttac?atattgtata 120

tagtgtcatg?ggtttgaata?aagacatatc?gatgtatctt?aatagcaggt?caattgatga 180

cgctcttgtt?gctccgacga?aaagcgactg?catcgggccg?atgcgggtcc?agggtcgtcc 240

ttatcaacgc?cccgtcagca?ctaacagttg?acatgtttct?ccaggtcggc?tagaaccgcc 300

ttttcgcgag?aaacgcgtgc?tatcgcgagt?gcagggcaat?tgttgcctag?cagtagctaa 360

agcctgagtt?ccgtgatctt?cacttctact?tcttcttctt?ccctgctccc?aacccattac 420

tctgcacccc?acgcccaaat?gcgtttccta?agcagtgcag?ccctattcgg?cctggcgtat 480

gcctccaccc?aggcggtcct?ccagccagag?gaaccatccg?acttccgtac?attccacagc 540

ccatattccc?cgcaccactc?gatccgcatc?cgccagcaga?atgaatcaat?ctgcgctgcc 600

cattccgccc?aatacaccgg?ctggctcgac?atcggccgta?aacatctctt?cttctggtac 660

tttgagagcc?agaatgaccc?tgccaatgat?cccctcactc?tctggatgac?aggagggcca 720

ggggggtcca?gcatgatcgg?tctgtttgaa?gaagtcgggc?catgtctgat?caatgagtac 780

ggcaatggca?cttactacaa?tccgtggggc?tggtcccgga?actcctccct?actatttgtc 840

gatcagccag?tcgatgtggg?attttcgtac?gtcgatgaag?gagaggacct?gccgggcgat 900

tcgcatcaag?ctgcaattga?catgcatcgg?ttcttgcagt?tgtttgtctc?ggaggttttc 960

ccgcaattgc?agactcttcc?cgttcatctt?tctggtgaat?cgtatgctgt?atgtatcttc 1020

tctaggtcac?ctgagctaag?actaacttcc?acagggtcac?tatgtccctt?acctcggcag 1080

tcagatcgtc?caacagaaca?agctctatcc?cactgagccc?caggtccttc?tgcactcatg 1140

tctcgtaggc?aacggctact?attctcctcg?cgacactacc?tacggctact?gggaaaccct 1200

ctgcaccact?aaccctggag?tccccgagcc?cgtcttcaac?cgaaccagat?gcgacatcat 1260

ggcggccaat?atgccgcgat?gcatggaagt?atccgacgta?tgtgttcgga?accccgatcc 1320

agctatctgc?catgctgcgt?cggaggtatg?ctacgagggc?gtgatcggat?ggtatgatga 1380

cgagtctggt?gaaggtggtc?ggaataggtt?tgatagtgag?ctttgcccca?tccagttgtc 1440

ccctgtcagc?aaatctctgc?aatttactga?tatgaaacag?taaccgctcc?ctgcgccctt 1500

gacggcatat?gctacatcga?ggccgctcgc?atcgagcagt?acctgaacac?acccgcagtt 1560

tgggctgctc?tatcaccacc?caaagaaatc?aaagaataca?aggttacttc?cgacaatgtg 1620

tcgcgcgcat?tcgatctcac?ttcagacacg?atgacgccag?cgtctgagca?agtcgcgttc 1680

ctgcttgcga?atcaggtaca?tttcctggcg?tatcagggca?atctcgatct?ggcgtgtaat 1740

acggcgggta?atctgcgctg?ggcgcattct?ctgccatgga?gaggtcaggt?cgagttcgcg 1800

tcgaaggcgc?tgcggccatg?gagttgggta?gatgtggtat?ctggaaaagg?tggagtggct 1860

ggaacgacga?aggaggtgag?agtgaaggtt?agtgagagta?cggataagga?gtcgaggttt 1920

gcgctagtta?cggttgatgg?ggcgggacat?tttgtgagta?tccctttcgc?cttgtgggat 1980

ccaagtatgc?ggattactaa?tggtctatta?caacttttac?agcttcctca?agatagacct 2040

gatatcgcgt?tggatatgat?ggtgcgctgg?atatccgggg?catcgtttac?tgagtgaagc 2100

atgaatgtcg?cagacgatgg?gataccggca?tatttcacag?gtttggtgta?atatatgtct 2160

tgtagctgtc?ttcagcagta?tttacaatat?gctgctgcct?tctctgattt?gggtataatt 2220

atagtttaac?acaatcaact?gcatcggcag?agatggatgg?gcttgatttc?gcacccgcag 2280

cagcgatgaa?tttccgtcta?accgagcgaa?cggtagcgga?cactaacact?gattgtattt 2340

acaaccctgt?ttctttcctc?ttgatttgag?acagaagtat?gtaagctagg?cgtagtagaa 2400

tacatttgtc?atctacttct?actgagttgc?ataacgtaag?cgactattgg?ccgctacctt 2460

gccccagtta?gttagttagt?agtagtgggt?gccgaagtca?gcacacattt?ttcagccctc 2520

<210>34

<211>1721

<212>DNA

＜213〉aspergillus niger

<400>34

attgatacga?ggcgtttgta?ggtggcgttc?ctgaagagga?tctgataccc?actggttttc 60

ccagtgggcc?tatgttttcg?tgatagtttc?ggcatttcta?tccatctagg?aaaattttga 120

tggcacctgc?tagtcgatgt?gcccttcctg?atggagcgat?ctcaaagtgt?caaatttctc 180

ggcgaaacgc?gtctacccct?tctttgggat?aattccctgg?tatgcagcat?tcctgacacg 240

agggattgtt?tgggattccc?cagattccac?gtagcggttg?gcaatgatgc?ttatgatttt 300

ataattctca?tcttgtcctt?tgtcctcggt?gcagtagaga?atcttcactg?cccgatacca 360

atatgacatt?gttactcaac?ttccacgcgc?tctttacagt?cattcttgtt?gccaatcttt 420

caaccagatg?cagcgcactg?ctctctggac?gtgacttttg?ctccacgcca?gcgcccggtg 480

agtcactccg?agcggagcat?aggaggctgt?atgatgtaca?ggcccaacgt?gacagcaccg 540

ccgaggagag?ccgggaggtg?gtgccttgga?ttgaaatcga?gacatggttt?catattgtaa 600

gcagcaatga?agcagcaaac?acagtatcag?acgacatgat?caccagccag?gtccgtcacc 660

attcaacttt?tgacccatgc?ctgcgcaatg?tcgtgttttg?gactaacaag?tcctagcttt 720

cctatcttca?gaaggcatat?gaaagtgcga?ctatcaccta?tcggttggag?ggcataactc 780

gtcacataaa?tgactcgtgg?gcgcgaaatg?atgatgaact?ggggatgaag?aatgccctac 840

gaaggggcaa?ttatggcaca?ttaaatgtct?atttccaaac?agatctccag?gcgtcatccg 900

acgagaattc?tcgggactat?ccaaatgacg?gtaaccgacg?aacagatgtg?tcagatcaat 960

catcatcaac?tgtcctaggc?ttctgtacgt?tgcctgaccc?gagtgtgaat?tccagcagcc 1020

ctcgttccag?ctacatcaag?gatggttgta?acgtgttagc?ggatatcatg?ccgggtggta 1080

gtttagcgca?gtacaacaaa?ggcggcacag?cggttcatga?ggttggccat?tggaatgggc 1140

tgctgcatac?gttcgaaggt?gaatcgtgct?cccctgataa?tgaaggagat?tacattgatg 1200

acaccccgga?gcaatctgag?cctacgagcg?gatgtcccgc?cgagaaagat?tcatgccccg 1260

atcttcctgg?ccttgatgct?attcataatt?ttatggacta?ttcatctgat?gactgttatg 1320

agagttttac?tccagatcaa?gcggagagaa?tgaggagtat?gtggtccgct?atgcgggaag 1380

ggaagtgacg?gacggactcc?aagggtatat?aatttgcaca?tataccagct?atagatagac 1440

aactatggta?gcacgaatat?tgatgtcaac?atcttggtct?ttcaattccg?tgggaattga 1500

ctatcacaat?tcatgaacct?tttgtagaat?ttgtgtattg?gagagcgatt?gatgcaaact 1560

agacactcgt?tcgaaattca?cagctagctg?tcatgtggaa?tgcgaaatca?gatcatcgct 1620

tgcgcttttt?tccacgatct?tcatcgtcca?ggccactttc?cctgtccttt?ttctttgcct 1680

ttgattcgag?ttcgtcccag?tcttcaccac?tctcttcgtc?g 1721

<210>35

<211>3550

<212>DNA

＜213〉aspergillus niger

<400>35

cctatcctct?gctccgcccg?gtggagagga?tctattgacg?ctatggttct?tgagcaatgg 60

cgacaagacc?caccagtgga?gataagagga?ccgaaactat?ggcttgttca?aggtctgctt 120

cgataaaccc?ctcatcacga?taaaccaagt?tgggcttact?aacaaggcca?atccttcaac 180

aatctcatcg?cacaacgacc?caaaatccag?caaggcacga?tccaacatac?cctgtaatga 240

caggaaacaa?attatcactc?ctctgggtgc?tgatgcagac?caactcagta?ttccgcgtgg 300

attgtccgac?tccgaagaca?agctttggtg?tctggtgctg?atcgtccgtc?gtggagccac 360

cactagtgga?ttgaattacc?cacacaccca?ctacttatac?tctttacgct?gtctatcatc 420

ttcgtgctga?tcagggggtt?attttcttgc?gctatttggt?ttcccactat?gcttctgttt 480

tgcagggctg?gtcataacaa?tttggtagtt?tgggttgggt?ggcttgttat?ggtcgatgtc 540

ggggaggggt?tcaccgttga?acaatatgct?tgtcgttagg?taggttgaac?tgatgcttgt 600

gttttgtttc?caggcgccct?ggacagcctt?tggctatcag?caatagctca?atagctggtg 660

ttttgctcgg?acgcgggtct?tggtcgggtt?gacccagtgt?gatctacgct?agacgctttc 720

ctgattggcg?atggatgtca?cgcctcttat?cagggtttgg?tgatcatatt?tagcctacat 780

aggcaggttc?ccttccgctg?ggtatggaag?tgcaaacgcc?agcgtatgga?gagtgaagtg 840

atgctagtca?atgggatcag?cagcgttctt?gtcaactagc?atatattccg?acgtttccca 900

ccgttcctga?tgatggcttg?catgccacgc?ttgctctgtc?gctgcgatca?acatgcatgt 960

ctcacttttc?ctactcagtg?ttacggcagc?gtttgccagc?ccaacacccc?ataactatgt 1020

tgttcatgag?cggcgcgatg?cattgcccag?tgtctgggta?gaagaaagcc?ggctggacaa 1080

aggtgcccta?ctgcctatgc?ggatagggct?tactcagtcc?aacctggatc?gtggccatga 1140

tttattgatg?gaggtgtatg?tgttccgact?cctcaccaga?accaagctga?ttgtccaggt 1200

ctcatccaca?atcgtctcgc?tacggaaagc?atctctccag?cgaggaggtg?cacgacctat 1260

ttgccccgtc?gaatgaggcc?gtcgagaccg?tccgaacctg?gattgaatcc?gccggaattg 1320

ctccaagccg?catctcgcaa?tcatacaaca?agcagtggct?acagttcgat?gcccatgcaa 1380

gcgaggttga?gcagcttctg?cagacggaat?actacatcta?cacccatgcc?gacacgggaa 1440

gttcccatgt?gacatgccac?gagtgagtcc?attttctaca?tgccatgaca?ccgctaacca 1500

gttaggtacc?atgtgcccga?aaccatccaa?tcgcacatcg?actacataac?accaggagta 1560

aagatgctgg?aagtgcgcgg?cacgccctcc?aaaaagagag?atgcagagaa?gcgctctctt 1620

ggcagtctgc?ccccaatctt?agcaccacta?ccaatcaata?tcacgaagat?tttcgacgac 1680

ccgctagcac?actgcgatct?ggcggtaacc?ccagactgca?ttcgaggtac?gttcatccat 1740

cccaattcaa?gatttcacgc?taaccgtcta?gccatgtaca?acatcaccaa?aggaacaaca 1800

gccacaaagg?gcaacgagct?cggcatcttc?gaggacctag?gagacatcta?cagccaagat 1860

gacctcaacc?ttttcttcgc?caactttgcc?aggttcgatt?actctccacc?ctcccttaga 1920

cacaactaac?accaccaagc?gacatcccac?agggaaccca?tccaaccctc?gactccatcg 1980

acggcgccac?cgccccaaca?gacgtcacca?acgccggccc?cgaatccgac?ctggacttcc 2040

aaatcgccta?cccaatcatc?tggccccaga?acaccatcct?ctaccaaacc?gacgacccca 2100

actacgaaga?caactacaac?ttcaaaggac?tcctcaacaa?cttcctctac?gccatcgacg 2160

gctcctattg?caacgaaacc?tcctctctag?accctcaata?cccagatccc?tccccaggcg 2220

gctactcctc?ccccaagcaa?tgcggcgtct?acacccccac?aaacgtaatc?tccatctcct 2280

acggcagccc?cgaagccgac?ctccccatcg?cctaccaacg?ccgccaatgc?cacgagttca 2340

tgaaactcgg?ccttcagggc?atcagcgtgg?tcgtcgcatc?gggcgactcc?ggcgtcgcct 2400

ccagcacggg?cacctgcttt?ggcgatgcag?acaacgtctt?cgtcccagat?ttcccagcca 2460

catgtcccta?tctcaccgca?gtaggaggca?catacctccc?cctaggcgca?gacgcagcca 2520

aggaccagga?aatagcagtc?acccgcttcc?cctccggcgg?cggcttcagc?aatatctacg 2580

cccgaccatc?ctaccagaac?cactccgtgg?agacctattt?ctccactacc?agcgacgacc 2640

tcacctaccc?ttactactcc?ggagtaaact?acacagactt?ctccaacaca?gatggggtat 2700

acaaccgcat?cggacgagga?taccccgatg?tttcagctat?cgcagacaat?atcatcatct 2760

acaaccaggg?cgaagcgaca?ctggtgggtg?gtacgtctgc?cgcggcgccg?gcgttcgcgg 2820

ccatgttgac?gcgcattaac?gaggagaggc?tggcgaaggg?gaagtccacg?gtggggtttg 2880

tgaacccggt?gctgtatgaa?catcctgagg?cgtttaggga?tgtgactgtt?gggtcgaatc 2940

ccgggtgtgg?gactgatggg?ttcccggttg?ctggggggtg?ggatccggtg?acggggttgg 3000

ggacgccgcg?gtttgaggat?ttgatggata?tatttgtggg?tgatgattga?tggctgagac 3060

aagatgtggg?aaggtgtaat?gaagatgatc?ctatttaatc?cggtaatcta?ccacacattc 3120

atattactag?tttatagcct?agtcagatat?ataacattgt?actactacta?tagaggcgtg 3180

tgatgcaaat?gctaaaatat?tgatatacta?ttaagcaact?acattatgat?ctaaacttta 3240

cagttcatag?tacagaaagg?ctttgatcag?tcctagttca?ataaaaggta?tagactactc 3300

actacatatt?aaacaccaca?actcgggatg?cttggggtat?agtgatctga?gagtcgcggg 3360

aatgcccaaa?tgctctgttc?gatcatatct?tggttgatgt?catgttggac?gcattatatc 3420

aaaagtcatc?tagcgctttt?ctgaaagttc?tattatgaga?attttgcttg?tttatagcta 3480

gcgggtggat?gattaatttt?gtatgagtga?ttctatagac?gttgatcatt?tccatgattt 3540

agaaactgag 3550

<210>36

<211>2280

<212>DNA

＜213〉aspergillus niger

<400>36

ctgcctctca?gcgaaaaccc?caaaaccaaa?catttctaga?tcagacaatc?aattgcggac 60

tgagttccct?gaatggtgga?agatgccata?ctgttagcgg?ggactatttt?tagcatgtca 120

tgacttggtc?ggcgtccgta?catagaaggt?tagcggatgt?cttgatgatc?acacaaaaaa 180

gggataggat?gaaactggat?gtctcactgg?ctatctcatt?tgcccaacag?catcagtcgt 240

tccggcccgc?tagttccacc?acatgtcact?tgtctcggat?tattctcgaa?ggtctggtat 300

ttcaatgttc?cacctggtcg?ctctacataa?atagaccttt?acgccgtttt?gttttcttct 360

tcgttctacg?tcgtttattt?atccaaaatg?gcttccaaga?ccctcctact?cattccggca 420

ctggccacag?ccgctctggg?aagtgtattg?gacctagata?tcaaggttca?aaatggctat 480

gtatgtcaat?gagaagctca?gctccctgag?aaatatctac?taactcggcg?aagaggacta 540

tcgaggtaga?ccttggaacc?ccaggtggcc?cgtttgattt?gatgtacgac?accggatcat 600

caacactctg?ggtgcttgat?agcaattgta?cagatgattg?tccaaatgtt?agcgggttag 660

ttgattcatc?ccaccattcc?ttaagcctac?actaatcacg?agcgccaggt?actcccgaca 720

cggctacaac?ctcacctcta?ctggtgtcaa?cttaggtgtc?aacgacagca?ttgcttacag 780

cggaggcact?gtcagcggct?tcactgccac?ggatattctc?acggttcccg?acaccaacgt 840

ctcatatcgc?cagagctttg?ccgtcattac?cgacagtacc?tgggcggcct?tagcagccga 900

tgggttcatc?ggcctggcat?cgtctaccat?cgcattcaag?aatactacga?cagccgtcga 960

acagatgatg?caggatggac?ttttggatga?acctcgattc?gccatatatg?caggttcagg 1020

ggaatcgacc?gtgaccaacc?ctaatccgga?gaataatggc?gtgttcacct?ttggtggcag 1080

ccatgaggaa?acctatgcgg?acggggaact?gcaatggatg?aagatgctct?ccccctttga 1140

aatatacaaa?acaaatctcc?ttggaattca?gggacacaac?aactccgatg?gccaggccct 1200

gtcaagcgac?gtcctgaact?ggtacggcca?ggttattttc?gataccggta?ggcattttct 1260

accacactgt?tttctttagc?ttgctagact?aatctattca?acgtcgcagg?tgcttcatcg 1320

ataagcattc?ccaacgacca?gattgaggcg?atgtatgccc?taacgccttt?ctcatacgct 1380

gacatctcat?ctggataccg?acctctgtgc?tccgatttca?atgatacatg?gtcgatctct 1440

tttacaatgg?gcttctatgg?cgagggtgtc?accttcaatt?tgaccggtga?tcagctggcc 1500

gtgcctggct?atcaggacga?cgaccactgc?ttccctccct?tcaatccatg?ggacagctac 1560

aacacgatta?ttggtcagca?ttggttgagc?aatttctatg?ctgtattcga?cttcggatca 1620

ttcgacccgg?agacatacga?tatacgtgtt?gggctggctc?ctttgaagaa?ggaatacctg 1680

ccgagcgctt?gacctaagaa?cttctattcg?tgtctttgag?catttagttc?aaacctcttg 1740

ctcggacgtc?aatgtttaag?catgcgtaaa?actatctact?agaagttaat?gttcatcgag 1800

agttcatcgt?ttaaataagc?agcttatctt?cattctttac?agccactata?cgcgacatga 1860

caattgtttt?atgggtcggt?gcgataaagt?ggccatctaa?aggcaatagc?aattaaaata 1920

agccaggaga?tggaacatga?aagaatctta?ctctttgtca?tggcctcatg?caactttggt 1980

gcgggctgtc?ggtgaccctg?tggggcacat?ctccggggag?tggaggccgt?taagcttcgg 2040

acaacattat?ttcttcagct?caaccatgaa?aaggttcggg?gacctgcatg?catcccccaa 2100

gcccgcaagt?ccgcgtcatc?aacaaatatt?gcgaagaaat?ggtacgcttt?agagccatgc 2160

gggctatatt?ttcggttcta?tagattgtta?gatataatta?ttgcttgtcg?aacccagatg 2220

cagcattgat?ctgttataat?cctcatattc?cgcgagtcac?ttgattccgt?gatcaagatt 2280

<210>37

<211>2287

<212>DNA

＜213〉aspergillus niger

<400>37

tagttgaagg?aggcttttgg?ctttcttcct?ctttatcttt?cttctgttgt?cagtgattct 60

gatcgcagaa?gatgtgcaca?agatgaagac?cgccccgagt?catctatgga?tactgaagag 120

atggaagaga?tctgacaacc?aaggagtttg?tattatccag?ttccagtttc?atgttttgcc 180

tccgtctgat?agttccacca?aagatactgc?cattttgtat?ccgtagttag?acggagacaa 240

attgacaacg?acagccttgg?gggccaagtt?cagacatact?tccagccgca?cgcatggatg 300

tataccttgt?tgctacgtga?gcgtacgcca?gcttatcgac?ctattttagg?acctgctccc 360

acgtggagat?tctgcagcaa?acgcaggaaa?caaggaccag?aaattcgtca?tcatcgtttc 420

tccttagcaa?tcggctagga?tgtttccctg?ctctcgtatt?tggtctctgc?tcgttgcagc 480

cgccaccgct?agtgctgtac?ccaccagtct?ggccaccacg?cacctgcaat?cggttgactt 540

gcttctgact?cgcagttctt?acgggtttct?tactgacata?gcccttggaa?ctccgggtca 600

gagcctgccg?tatctggttg?actggacctg?gaccggccac?tatgtggtga?ccaccttgtg 660

ctacaacgat?cccaccgcca?cctacgattg?tctcaacgtc?gatcagaaaa?ttttcaacca 720

gactttgtca?tccactttta?tcaaccaaac?tgaccagtat?ggctatcttt?actgggatcc 780

caaccacttc?tactttacgg?agcccgcagc?agccgatgtg?gcgacggaca?tgctgcgcat 840

cggtcccacc?gcggtgaaca?ccaccatcca?agcagccaat?ttcgtcttca?acgagactat 900

tagcgcattc?cctttctcgg?gagtatatgg?actctcacct?gtttttcagg?gtgacaatcg 960

tgagttgttt?ccccaccgtc?ataaaactaa?ttcatgttaa?cacgaatcat?aggatccgtg 1020

caagcgtcct?tctaccaagg?atggaggagc?ggcgcctggc?actctccaat?tgtctctttt 1080

atctactgcc?acgacaatgc?caccaaagcg?gtatgcagtg?gttacgacgg?ccttcagaca 1140

ctaggcggat?acaacacctc?tcacgtccag?ggagatatca?cctggtacga?catcattgtc 1200

acggaggcga?tcaacacgct?ggactttgtc?tatgcgccag?ccgtgattaa?ttattgggcg 1260

ttgaacctca?cgcgcttctc?tatcggagac?gaagagcaag?agctcaacaa?gaccactact 1320

ctggatggaa?agcaagccgc?cgttgccgcg?ttcgaccacg?cttcgtatgg?tcgcggtgcc 1380

ccagtgtctg?tgtacggtta?ccagcgtcta?gtcgagctgg?tcggggcaaa?agccgtcacg 1440

ctttccgatc?ctccaaataa?cggtgagcag?ggattctatc?agttcgattg?ccggaactcg 1500

agtttactgc?caccgctgcg?gtatgagttt?gccgggtcag?agcgggcgtg?ggagattgtg 1560

cccgagaact?atgtggaggt?gctggcgaac?ggaaccaata?agtgcacctt?taatgtacgc 1620

accctgggag?atggagcgat?ggtaatggga?aattttggcg?agacatttgc?cattgataag 1680

tatgtcatgt?ttgactttga?gaagttgcag?gtggggattg?cagacttcgc?gtggtaatgg 1740

tataaggatg?tgtgtttgaa?catgtgcatt?atctcgagtt?tgactgaata?gacggagaat 1800

ccaagaccgt?agatcatgat?gctatctgct?tgattatttt?caatgtctct?agtatgggga 1860

aagtaataca?tgtgataata?tctccaaagt?agtaattaaa?agagaaaact?aatcaccact 1920

agtaattaat?actgcaaata?gcgctgttcc?acattgagac?aaccatataa?taataagtta 1980

gtggatatac?ggaatattag?aggtcatctt?aataccatac?tagtggatac?aaccatccgt 2040

acatacggaa?taggaaggag?gagcgagatg?gtcatcattt?gacactgagc?atactgtgtt 2100

ggcccagtca?agctgttgta?tgggggagct?cctagtgtgc?ccaaggctcc?agcatcttca 2160

ccttccgcaa?cgactttact?tagtttacat?taggtgagcc?tttctgttac?atagttgatt 2220

gaaagcctct?ccccaattgt?cttagcatga?tagaggttac?tcggagaacg?tactaatcag 2280

gaggcca 2287

<210>38

<211>1950

<212>DNA

＜213〉aspergillus niger

<400>38

gatctttcta?gagcattgtc?ttgattgtgt?cattctgtca?ttgactccgg?ctatgaaata 60

ttattctcaa?tctgcctaaa?accaaattct?actctatcac?tacacatttg?tatcacctga 120

tctggctgag?ataggagagt?ccggcatctc?atcgtctgca?tcagacaatt?gcgataaatt 180

cattgcttgc?acctgttatt?gattcttcca?agttatgcat?ctcccacagc?gtctcgttac 240

agcagcgtgt?ctttgcgcca?gtgccacggc?tttcatccca?tacaccatca?aactcgatac 300

gtcggacgac?atctcagccc?gtgattcatt?agctcgtcgt?ttcctgccag?taccaaaacc 360

aagcgatgct?ctagcagacg?attccacctc?atctgccagc?gatgagtccc?tgtcactgaa 420

catcaaaagg?attcccgttc?gtcgtgacaa?tgatttcaag?attgtggtag?cggaaactcc 480

ctcttggtct?aacaccgccg?ctctcgatca?agatggtagc?gacatttcat?acatctctgt 540

cgtcaacatt?gggtctgatg?agaaatctat?gtacatgttg?ctcgacacag?gcggctctga 600

tacctgggtt?ttcggttcca?actgcacgtc?cacaccctgc?acgatgcaca?ataccttcgg 660

ttcggacgat?tcttcgaccc?ttgaaatgac?atcggaagag?tggagtgtgg?gctatggaac 720

tgggtctgtc?agcggcttgc?taggaaaaga?caagctcacg?attgcaaatg?tcactgtacg 780

catgactttc?ggacttgctt?ccaacgcatc?ggataacttc?gagtcgtacc?caatggacgg 840

cattctcggt?ctcggtcgaa?ccaacgatag?ttcctacgac?aacccaacat?tcatggatgc 900

cgttgcagaa?agtaacgttt?tcaagtcgaa?tatcgttggc?ttcgcccttt?cacgtagccc 960

cgccaaggat?ggcacggtca?gctttggcac?tactgacaag?gacaagtaca?ccggcgatat 1020

cacctacacc?gataccgtcg?gatcggacag?ctattggcgc?attcccgtgg?acgatgtcta 1080

tgttggcggc?acttcatgcg?atttctccaa?caaatcagcc?atcatcgata?ccggaacttc 1140

ttatgctatg?ctgccttcaa?gcgactcgaa?gacgctgcac?agtctcattc?ccggcgccaa 1200

atcttcgggg?agctaccaca?ttattccgtg?caacacaact?actaagctac?aagtggcatt 1260

ctctggtgtg?aattacacca?tctcgccgaa?ggactacgtg?ggagcaactt?caggttctgg 1320

atgcgtttcg?aacattatca?gctacgactt?atttggtgat?gacatctggc?tcctgggtga 1380

cacgtttctc?aaaaatgtgt?atgctgtgtt?tgactacgat?gagttacggg?tcggatttgc 1440

agagcgttcc?tcgaacacca?cctctgcgtc?gaactctacg?agctctggaa?caagcagcac 1500

ctcgggatcc?actacaacgg?gcagctcaac?gactacgacg?agctctgcta?gctctagtag 1560

ttcatctgat?gctgaatcag?gaagtagcat?gaccattccc?gctcctcagt?atttcttctc 1620

tgctctggcg?attgcttcct?tcatgctttg?gctctagtta?accgcatctt?actcgacgcc 1680

tgaacctcgg?gaaacatatg?cattatttac?acatgctgct?gatttgtatt?tgcatatatt 1740

cttcgagcct?ggacggcgtg?cgggtcatat?taccttacat?tcgaagtcct?tctctaatca 1800

atcaacattt?attcttactc?caccagttct?ggctcgcaat?taaccctgtc?taagaaaaag 1860

ttggtataga?acatggcatc?cactacctgg?aacattcaaa?gaaccttgtc?cgggatcagt 1920

gtgtatgact?tcgggtacga?ttctgacatg 1950

<210>39

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>39

accttctggg?gtggattatc?tgaagagatg?aactgccccg?cccaagtgca?agaagatgat 60

gcctgacagt?tgatgcctga?gggatgcttg?tatcaggtga?tcccggctat?ggtggtctaa 120

cccagttggg?ctgaattgac?cacatttagt?tagtgtcagc?cttaaacaga?tgaggctttt 180

cgcatgattc?atttccaagg?aacccaagga?aatggtcaaa?cagcaaagga?ataatgaccc 240

aacagttata?cctagctatg?cagcataaaa?gagaaaaaaa?acttgaactg?atacgagcca 300

tcatgtaacc?tccgtctctg?gctcaattct?ccatccaatc?acaattcttc?caagcaaact 360

tgggattcgc?cgggttctcg?gcaaccctaa?cctctaccag?taaaggttga?tccttccccc 420

acccgatttc?gcatggcttg?gacccctgaa?atatagttcc?tctacgtgcc?tgaatatgga 480

gcacgttatt?cgtctttcag?ggtcttgggg?ccttggcctt?ggggctgcca?agcgggcggc 540

ggagtgtgcc?ccgtggtgcc?ctgttttgag?cttggatgcg?attactatca?tcacttaata 600

agagcaagac?cctgcgtcga?tattgacggt?caatacactg?acctcagaga?accgtcgatg 660

tgcctgattt?cgctccatca?aaatgacgtc?ttctaccttg?cgccttgccg?tcgcgttggc 720

tttgtcaact?tgcagcagtg?ccctatcgag?ccagcgagat?gattcacttg?tggttccatt 780

tccttttggc?aatcttgagg?atgtccatat?tgccaagcgg?gatagctcca?agacagtaga 840

agctcctcta?gtgatatatg?tcagtcagct?ctcccactct?ccatctatgt?catcaactcg 900

acaaccctaa?caatcaacag?ggcgacagct?actggatgaa?cgcctcaatt?ggaacccctg 960

cgcagtcact?aagtttccta?ctagatctta?cgcgctcaag?ggtcgagccc?gcatacaccc 1020

tcgatgagaa?ttacgaatgt?tctgacgatg?aactctgctc?cgaattcggc?ttctacaaac 1080

ccaccgattc?atccacttat?cagcatctca?cctacacaca?gagacacgat?gcaggtgtcg 1140

actactccta?ccttgatacc?ataactcttg?gagatcacgc?aaccgacaat?gtcccactgg 1200

acatgtatct?tttgtcctac?atttcctgtc?agttccccct?ctctccctta?ccaccctatt 1260

tatccatcat?ctgaccattg?tctaaccacc?acatagacag?ctccctcggt?ctctcctccg 1320

tcaacaccag?cttcccctac?atcctggtcg?atcgcggcct?caccacctcc?ccatccttca 1380

gcctaatcgg?cgacaacgga?aacaccacca?cccccagcat?catctttgga?ggcatcaaca 1440

cctccaaatt?caacgggccc?ctgcaagcct?tctccttcgc?agaccacagc?atcaccaaca 1500

atccattcgt?caccgtcgaa?gctgactccc?tccaactaac?caccaacacc?aacgataatt 1560

ccacctaccc?tattccctcc?tccaccccca?tgatgctcag?aaccgaagaa?ctaatcacct 1620

acctccccaa?ctcgaccgtc?caatccctct?acaccgacct?taacataacc?atggacggcg 1680

tgatctccac?ttcaagattc?tacggggtcc?ttccctgcgc?ccgccaggaa?accgaatctc 1740

acacaatctc?tctagccatc?ggcaacatga?ccttctctgt?gtcctgggat?gagctcttcg 1800

tcccgtggac?gcgtgacgga?ctatgcaagt?tcggcattca?ggcccaggat?tcagattaca 1860

aaactcgtgc?ggagctgggt?gttccctttc?tgagacggat?gtatgtcgct?gtggattata 1920

ataatcagtt?tgtgggcgtt?gcgacgctga?aggatgatga?tgatcagaat?ggaggtgaag 1980

atgagattgt?ggagattggc?actgggacgg?cgttgcctag?tgctgtcggg?gattggccgg 2040

ctagtgttac?ggcgtatacg?cctgctgctt?ctacagggac?ggcggctgcg?acgttgacat 2100

tcacgacggc?gacgtctagc?gggggaggtg?tggtgccgac?gggtctatca?gagttgggta 2160

gggcgttttt?ggtgccgggg?gtgctgggga?tggctgtttt?gcaggctgtt?taggttagag 2220

attgtggtac?gattattctg?taggttaaag?atgtatattg?tctgttattg?tacttgatag 2280

cttgatatgt?ataatgaaag?tagtatagtt?tgaatttttg?ttatacacca?cgtgaaccca 2340

ctggaccgtt?gtctactgct?cgcagtgagt?gatatagtgt?agacccgtat?tactagtcat 2400

gtaggaccct?accttgatac?cataacactc?ggagaccacg?caaccgacaa?cgtcccactg 2460

gacatgtacc?ttttgtccta?catttcctgt?cagccccttt?ttgaatgtac?ctacatccct 2520

ctaaactcta?gtactatcac?gaacagcaaa?tgacattcaa?tctattctat?gcatattacc 2580

cttctaacaa?tacccagatt?ctatcaaatc?acgtgaacat?acagccctcc?caagtcacct 2640

gacaaagcgg?acaacaatgc 2660

<210>40

<211>2501

<212>DNA

＜213〉aspergillus niger

<400>40

tgattcccaa?atcgcaccag?atgaagttga?atcagtggta?cagtttagcg?ggtggctgtc 60

atgacagaca?gaccctcaaa?ttgaagcaat?ggcagataga?tatggtctcc?agtccaccga 120

ttgaatgcag?ttggagaagt?caatcccccc?ttggaaagcc?cccaacgttg?cgctttctga 180

ccacctgaaa?tctgacaatc?agcaatcctt?cagctcttct?cagcgtctct?tggggccgat 240

ccccatcgga?tgaacacgcc?tcatccctca?ttaggcatgg?ctgagctgca?tgtcagcgga 300

gtcccaacac?ccaaaccaat?cctttagcgc?gcaccgggcc?tgcgaagcca?tcaccatcgc 360

ccgccttgca?gcaatgtgct?ccccggtcgc?attcctttcg?tttcataatc?tcacctccgg 420

ccagcccata?tccttcatct?cgcagctttt?ccctactcct?tcttttcttt?tgctttcttc 480

ccccatcgca?ggctgtttga?atgcctgctg?aagtatccat?ggtatgatgc?gaccgatact 540

tctcccccta?ctgggggtat?ttctgcagac?ctcctcggca?tccaatccct?atgtaatgag 600

ctggtcttcc?caagcctacg?gtccagatgg?cccgtggcag?gccgtatcca?tcgacgtggg 660

cagcaaccag?cagacggtcg?atctttaccc?cggagccaac?tatgctagca?cgatcctgat 720

gagcactctc?tgcacgaaca?aaaccctgtc?atccacctgc?tacgctgccg?aagcaggcac 780

gttcaaccaa?aacacctcca?ccactgccta?caccaccgcc?agctcgtggg?aaacaactta 840

ctgggccgtc?gagggtggaa?gccaagaggc?tgtgctcggc?gatgaggtca?ccttagggtc 900

gtttgtcgtc?cccaatgtga?gcttcgaagc?catctaccag?acctaccaga?cctatcccaa 960

tggcatcgcc?tatcctgtct?cggtcggcag?tctggccctg?gggggtccgt?acttgtcgga 1020

taccgtctcc?aattcgacgg?tcctgaacat?gatcgcagga?tggctttact?cgtccaacga 1080

cattccgtcc?tactcgtacg?gcatgcatat?cgggtcggta?gaccccaaaa?tcccaggctc 1140

cctgatcttg?gggggctacg?ataagagccg?agtgatcgga?gacgtgagtg?cgcagggagt 1200

agtgtcttcg?agtggtcttt?tggaacttga?attaaaggat?attgggctgg?gtgttgcggc 1260

gggttcctct?cccttcagct?tcaacaacga?aagtggcttg?tttctccaaa?gcagtggttc 1320

ggttcaggcc?aagaccgtcc?agattgatcc?aaccaagccc?tacatgtacc?ttccccaggc 1380

gacatgcgat?gccatcacct?ccaccatgcc?gatctccttc?aattccagct?tggggctata 1440

cttctgggac?accacgagcg?atgattatct?gaatatcacg?tcttccgccg?catacctctc 1500

ctttgtgttc?aacatgaatg?gggtcaacaa?caagaacatt?accatcaaga?ttcccttttc 1560

ccagctcaat?cttacgctgc?aagaaccgct?ggtcgatcaa?aacgtcacct?acttcccgtg 1620

cttcctcact?acctccaccc?cggtgctcgg?tcgagccttt?ctccagtccg?cattcgttgg 1680

ggtgaactgg?ttcaacggga?acaactcggg?cacatggttt?ctggcacagg?cccccggccc 1740

gggttacgcc?agtgaagaca?tcacccggat?cgcagtgagt?gacacgtcgc?tttctgcctc 1800

taacggtacc?tgggaagaga?cctgggctac?gtactggggc?atcaaaacat?ccgacaactc 1860

gagcagctcc?aagagtggcc?tgtcttccgg?tgccaaaatt?ggaattggcg?tcggggtggg 1920

tgtcggtgga?gcagtgttga?tcgcagcagg?tatagccatt?gcattctgtc?ttcgccgtcg 1980

ccgcggggcg?agtcaagagg?cggctggaga?gcaacggagg?tcgatgttta?ggggctttgc 2040

ggagctaccg?ggaggtgctc?acagtgaacc?ggcgaaggag?ttggatacga?agatgcataa 2100

gccgccgcag?gaaatgatgg?cttcgcagga?ggtagagcga?tacgagctgg?ggtgatggct 2160

aattctaaat?tttaatatgc?gaagggatcc?ctacaggata?ctacaggata?ccatatacgt 2220

gcgaatataa?tagctagttg?ttccaatatt?atgtatcaag?cttatactgt?ggattaacca 2280

acgacttgtc?tccgatacat?agggtagtac?gtagtcatgg?atccggggtg?tattgtcatt 2340

cagcatgacg?cattgctcaa?gccaaaagta?agctgggcca?gtgtatcacc?catgaaagca 2400

accctctctc?acctctgtac?tgatgacttg?gtggaaattt?ggatcctcgg?aaattgtgat 2460

taacacgtgg?atctagaccc?gaccaggcgg?gagcttggca?t 2501

<210>41

<211>3570

<212>DNA

＜213〉aspergillus niger

<400>41

gaaaaggatt?gtccccatgc?ataataggcg?ttccaccacc?tgcatatcca?tacatgcagc 60

ccgagttatg?cgatgtgatc?gggcctggaa?taacatgatc?tccgatctaa?cgggggccca 120

aaaggggcag?ccaagatctg?ctaggcccaa?ccaggctagc?ggcgggccat?ctgaggcggt 180

gatccatttt?gggcgaggaa?aaggcaaaaa?gtggatcggc?gaaaggcaag?ttggagtgtc 240

gggagtttgg?tctcccgccc?cagggccgcg?tattagtcta?gctctctttc?tattcactcg 300

ggccaactcg?tcaactctct?ccctcgcttt?cattcagatt?tcattatttc?tcttacggtc 360

ttgccattct?tgccactttc?ggtccttatt?attgcttttg?atccccagtt?cccttttccc 420

aactgggtgg?cgctcctaca?gaccgacaca?tacctggcgg?actttccttg?cttctggctg 480

tacaccggga?cccgcctcat?acccagtacg?tgttggtcca?tggcaagcct?cctacttggc 540

ctgattacat?cgtcctgaga?gagagagttc?accaaaactc?tcccccaaac?gatgcgtctt 600

acaggtggtg?tcgctgcggc?tctgggcctc?tgcgctgctg?cctccgcttc?tctccatccc 660

catcgttcct?acgagaccca?tgattacttc?gctctacacc?ttgatgaatc?cacctcgccg 720

gccgacgtcg?cccaacgact?aggtgctcgc?cacgaaggcc?ccgtcggaga?attaccctca 780

catcatacct?tctcgatacc?ccgtgaaaac?agtgacgatg?tccatgcgct?gctggatcaa 840

ttgcgcgatc?gtcggaggtt?acgccgccgc?tccggagatg?acgccgctgt?ccttccctcc 900

ttggtcgggc?gagacgaagg?tctaggtggc?attctttggt?ccgagaagct?ggctccccag 960

agaaagctcc?ataaaagagt?gccgccgaca?ggatatgctg?ccagatcgcc?cgtcaacact 1020

cagaatgacc?cccaagcgct?tgcggcgcag?aaacgcattg?cctcggaatt?gggcatcgcg 1080

gaccccatct?tcggcgaaca?atggcatttg?tataatactg?ttcagttggg?ccatgatctt 1140

aacgtgacgg?gtatctggct?ggagggcgtt?acagggcagg?gtgtcacgac?ggctattgtc 1200

gatgacggtt?tggacatgta?cagcaacgat?cttaggccga?actattttgc?ggcgggttct 1260

tatgactata?acgacaaagt?accagagccg?aggccgcgct?tgagcgatga?ccgccacggt 1320

actagatgcg?cgggtgaaat?cggtgcggcg?aagaacgacg?tgtgcggggt?tggtgttgcg 1380

tatgatagtc?gcatcgctgg?tattcggatt?ctctccgcac?ccattgatga?cactgatgag 1440

gctgcggcta?ttaactacgc?ctatcaggag?aacgatatct?actcgtgttc?ctggggtccc 1500

tatgacgatg?gcgccacaat?ggaagccccg?ggcaccctga?tcaagcgggc?catggtcaat 1560

ggtatccaaa?atggtcgtgg?tggaaaaggc?tcggtttttg?tgtttgcggc?tggtaacggt 1620

gccattcatg?acgataactg?taactttgac?ggttacacca?acagtatcta?cagcatcacg 1680

gtgggtgcca?ttgatcggga?gggtaaccat?cctccgtatt?cggaatcctg?ctcggcgcaa 1740

ctggtggttg?cctacagcag?cggcgccagt?gatgcaattc?ataccacgga?cgtcggcaca 1800

gacaagtgct?cgactaccca?tggtggaact?tcggcggccg?gcccgctcgc?tgcgggaacc 1860

gtggcgctgg?ccctcagtgt?gcggccggaa?ctcacctggc?gtgacgttca?gtatttgatg 1920

attgaggcgg?cagtgcctgt?tcatgaagat?gatggaagct?ggcaggacac?taagaacggg 1980

aagaagttca?gccatgactg?gggatatggt?aaggtcgaca?catatacgct?ggtgaaacgg 2040

gcagagacct?gggatctggt?gaagcctcaa?gcctggctcc?attccccctg?gcagcgggtt 2100

gagcatgaga?tcccacaggg?cgagcagggc?ttggctagtt?cgtacgaggt?gacggaggat 2160

atgttgaagg?gagccaacct?ggaacggctg?gagcatgtca?cggtcaccat?gaatgttaac 2220

cacacccgcc?gaggcgatct?cagcgtggag?ttacggagcc?ctgacggtcg?ggtcagtcac 2280

ctcagtacgc?cccggcggcc?agataatcaa?gaggtgggct?atgttgactg?gaccttcatg 2340

agcgttgctc?actggtaagt?aaactttttc?tcggttgtcg?gttcttctgc?taatacatat 2400

ctaggggcga?gtccgggatt?ggcaaatgga?ctgtgattgt?caaggacacc?aatgtcaacg 2460

agcatactgg?gcaattcatc?gattggcgac?tcaacttgtg?gggcgaggcg?attgacggag 2520

ccgagcagcc?tctccacccc?atgcctactg?aacacgatga?cgaccacagc?tatgaggaag 2580

gaaacgtggc?taccacgagc?atcagcgccg?ttcccacgaa?aaccgagctg?cctgacaagc 2640

ccactggtgg?cgttgatcgc?ccggtgaacg?ttaagcctac?aacatccgcg?atgccgaccg 2700

gtagtcttac?agagcccatc?gatgatgaag?aactccagaa?gacccctagt?acagaggcaa 2760

gctcaacacc?aagtccttct?ccgaccaccg?cgtcagatag?tatcctgcct?tccttcttcc 2820

ccacgttcgg?tgcgtcgaag?cggacgcaag?tttggatcta?cgctgcgatc?ggctccatca 2880

ttgtgttctg?cattggcctg?ggcgtctact?tccatgtgca?gcgccgcaaa?cgtattcgcg 2940

acgacagccg?ggatgactac?gatttcgaga?tgatcgagga?cgaggatgag?ctacaggcaa 3000

tgaacggacg?gtcgaaccgt?tcacgtcgcc?ggggtggcga?gctgtacaat?gcttttgcgg 3060

gcgagagcga?tgaggaaccg?ttattcagtg?atgaggatga?tgaaccgtat?cgggatcggg 3120

ggatcagcgg?cgaacaagaa?cgggagggcg?cagatggaga?gcattctcgg?agatgaagtg 3180

cagtagatga?gggttgattt?tatttcggac?agtgtttctt?acttgttgga?tgacctgcgt 3240

tgaacaatat?tcctgctgtg?tatgctgcat?agagaaagcg?tgtatatacc?atgtatgtgt 3300

gcatcatctt?tgatcgggtt?attattcttc?atctgccatg?gtttgtgatc?tccggaatag 3360

taccaaagga?acactaaatt?aagggtcttg?gcgatgacgc?ttcccgtcgc?tgcttttgac 3420

ttcctccgca?tctcgtctct?cctgctgttg?accgcccgcc?aaccaacctc?catctcctca 3480

ctcctcccac?cttaatcttg?ctgtcctgct?tctagagccc?cccagtttaa?tttaaaaacc 3540

ggcttttcct?agctccacgt?attgtacctc 3570

<210>42

<211>1236

<212>DNA

＜213〉aspergillus niger

<400>42

aggtcccgga?cagatgacaa?ggctctaaga?ctgtatgtct?cattcagggt?ctagccgtgc 60

ccgctcttat?tggcccggac?gcttcacgcc?gcgattactg?cgcccccgca?tgtctctctc 120

tccgctcgct?atcgatgcgg?tgtttgcccg?ccattgatgg?gaggaatgtc?tcacttcggg 180

cttgtgcttc?gcagatccat?acccctcaat?gacctagtat?agcaaacagg?cgtgatgtca 240

atgattgtgt?ttctattcag?agcatcaggc?ctcccatgtc?tatacctcag?ttatggagcc 300

ttggccgcct?tgggtaggca?aggcctcccg?ggtattctca?ttgccatttc?accgctactg 360

cagcttcaag?tgaagagaaa?gaggggaaag?aaagaatata?agtagcttga?caatctcctg 420

cacgtcctcc?tcttcagctc?acatcaaaca?tctattcttc?actcctcaag?agtaaaccac 480

tactaccaaa?cacaatctca?tctatagctc?acgagtacca?gcatcactca?tctaccacaa 540

tgaagacctt?ctctaccgtc?acctctctcc?tcgctctctt?ctcctcggct?ctggccgcac 600

ccgttgacag?cgctgaagcc?gccggcacca?ccgtctctgt?ctcatacgac?actgcctacg 660

atgtctctgg?agcttccttg?accaccgtct?cctgctcgga?cggtgccaac?ggcctgatca 720

ataagggcta?ctccaacttc?ggctcccttc?cgggcttccc?caagattgga?ggcgccccta 780

ccattgcagg?ctggaactct?cccaactgcg?gcaagtgcta?cgccctgacg?tacaacggcc 840

agacagtcaa?cattctggcc?attgattccg?cacctggtgg?cttcaacatc?gctctggagg 900

ccatgaacac?cctcaccaac?aaccaggccc?agcagctggg?tcgtatcgaa?gctacctata 960

ctgaggtgga?tgtcagtctt?tgcgcataaa?cgccaaacat?tcagcaaatg?cttgggggat 1020

gaattcggcc?atggcaggga?atggttatag?ttggatatga?tttcgggatt?tacgattgat 1080

acgcttattt?gggatacttt?cctttatctt?acctattgtt?aatagcataa?tacggagcat 1140

cacatacgta?catacacata?catcaatata?gtgatatatg?aacttgaata?cagagctttt 1200

tgaaaatgaa?atgagagatt?ctaaggtcgt?atctac 1236

<210>43

<211>1750

<212>DNA

＜213〉aspergillus niger

<400>43

gtgaaagata?tcggatagcc?gtagaccctg?aaagattatt?tgagcctaag?ggctcttgca 60

gtgacgctag?caacagcgaa?cccactggga?tccaaaacac?tagatgaagc?tattgtaagc 120

cggaaccctt?ccggcgcccc?acccagctcc?tgcaggggcg?aaaacatgac?tctctaactt 180

aggagaaatc?acagccaagg?cccaagttca?ctgagagacc?gaaatggcgt?gaatttaaat 240

gttgcctttc?gcgcacttta?ctattccctt?gttcctatca?gcagtcactg?cttggcctcc 300

tttctagtga?gcagttccat?tcactttagg?ccccacggct?tcgttacaag?agcagtatgg 360

ctcaaatatt?ctggctttca?ctcttcctgc?ttgtctcttg?ggtcagagcc?gagtccaacc 420

gcaccgaggt?ggacctgatt?ttcccaagaa?atgatacctt?tgcgccaatg?cctttgatgc 480

cggttgtatt?cgccgttcaa?gccccttccg?tcgcccataa?agttaataca?tacatcgagt 540

acggctatta?cccagtaggc?cgtccaaatg?aaacagttat?tggccagacc?gaccatgtgt 600

ccgactcaac?aaacgaaacc?acttatttca?gtgtctctgg?tatcggcaga?acgttcaata 660

ccactggcag?ctgggagctg?ttttggaggc?tgagatggac?caattgttca?atctcagaag 720

actcgagata?ctacaaccaa?tcctacccct?ggatatcctc?cccatacatc?gacggtagcc 780

tcaacatcga?caaggtctat?gagggctttc?actacacagc?atacaatgtc?attgtcgaca 840

gggttacctt?cagcactcgc?gaagatgcta?gccaacccaa?cctcacgacc?ctcaccaata 900

gcgagaactg?cgataaagtc?tcgtctcttg?ctctattgtc?gattgtggac?tccctaagga 960

ttccacccca?gttaccccaa?gaagatattg?ataccgtgtc?aatgtgccca?caactcgccg 1020

atgccaggct?aaattcaact?tcaacttcaa?gcccctgcag?cgttagcatt?agtcccgagg 1080

ttgagtctaa?tatcctggcc?aagatcgcag?acaatgaatg?caataacgca?cttcaccccg 1140

ctgtgagttg?caccactgaa?gaaaccaagg?aaggcagcgc?gagcagccat?gaccacggcc 1200

atgctgtatg?gcttgtcatt?acgctagctt?ttgccttcct?tttctaaagg?atccaatgct 1260

agatgtgctc?gactcaggct?ttgagacagg?tagacaggaa?aacaggcttt?attgtcacgt 1320

cgccagtagg?ggaccatcaa?ccacacatat?gtccctacga?gaccatagct?atcaggaaca 1380

atgtacgggc?tggaactcca?ctcactctta?ggttgtaaat?cccatttctc?aatacatagt 1440

ttgtgagcaa?accacgcttt?tcttgaatca?ttgcatcctc?tttagcttca?attccttacc 1500

cacaaacacg?agaggagcgg?acccgccatt?ccacatagtg?tcttggcagg?gtcgcctctc 1560

caactacaat?gcccaacttc?agaggcccag?actatctctg?ccaagtgcca?aggctggact 1620

caaattacgg?aactctcgga?tgcttgtgcc?atggcttatt?gcttgaaggg?tttggaggtt 1680

tcggtggtta?gcgcccggat?aggcagtaga?acacctccaa?atgaggaatg?cggccgaagg 1740

taggtggggc 1750

<210>44

<211>2030

<212>DNA

＜213〉aspergillus niger

<400>44

tggatgccga?gcaaattaat?taactgcgct?tacatggctg?tcctaacaaa?tcccttggca 60

ctcttagcag?tggtgcctgg?atgggacgaa?gccctgggca?tagcttgttc?atcggtaggc 120

cgaaactctc?ttttacgtac?cgtacggcca?atatcagtgt?gaacccgcaa?tttagatact 180

attaacttac?aagtaccctc?acgttatgta?gccgaatgtt?taaaagcgat?ggaccggttt 240

aaagtctccg?ttgtgcgatg?gctgttatta?acttggatta?acttgcaatt?tgcatcaagt 300

aagacgtgct?tctttttgga?tctttttaca?cacattactt?acggcggatg?ctctcgacac 360

tccggatttc?accagggtgg?tgttagtcca?ccatcggact?ccgatgcatc?ttaacatggg 420

tggtcgagat?gtcgccattc?tcagcaggca?ctttgctgtg?acatcctcac?aaagtgttaa 480

tggcgttgtc?tctgggatgg?tatgaggaag?aatgatgcca?tccgtgaaga?gtgtcgactt 540

gatagatact?caacaaaaag?tcatcctata?agtattgggt?tggatctgct?gttcttatca 600

tttcgtctcc?atcccactgc?aatcttcttc?aagacttcaa?agtccatagt?tccaacacac 660

agtcacctct?tcacccagct?tcactaccaa?ccaattcttc?aagaagaagt?tcactgctgc 720

aattgctact?gccattttcg?caagcgttgc?cgtcgcagct?ccccagcgtg?gcctcgaggc 780

ccgcctcaag?gcccgcggca?gcagcaaggg?atcccgaccc?ctccaggcag?ttgctagacc 840

tgcatcaacc?aagaaccaga?ccaacgttga?gtacagctcc?aactggtccg?gtgccgtgct 900

ggtggagcct?ccctctgctg?cagcgaccta?cactgcggtg?accggcacct?tcactgtccc 960

tgagcccacc?ggcaactctg?gaggcagtca?ggctgcatct?gcctgggttg?gtatcgacgg 1020

tgatacctat?ggaaacgcca?ttcttcagac?cggtgttgac?ttcaccgtga?ccgacggaga 1080

ggcctcgttc?gatgcctggt?atgagtggta?cccggattac?gcctacgact?tcagcggcat 1140

cgacatctcg?gcaggcgatg?agattgttgc?cattgtggag?tcctacacct?cgactaccgg 1200

tattgccatt?attgagaaca?agagcaccgg?ccagaaggtg?tccaaggagc?tgtcgtccag 1260

ctccagcctc?ggtggacaga?acgctgagtg?gattgtggaa?ggtaatatga?aacatacatt 1320

cctcattcaa?cattaccaaa?cgctaacgcg?atcttagact?tcgaggaaaa?tggttcgctc 1380

gtcaacctgg?tggactttgg?caccgtcacc?ttcactggtg?ctgttgccaa?ggcggcgggt 1440

ggtgagagtg?ttggacttac?cgatgcgacc?atcatcgaga?ttgaggagaa?tggccaggtt 1500

gtcactgacg?ttaccatcga?cagcgactct?gaggtgacca?tcacctacga?gtaaatttgc 1560

acacgaggcc?tgtcactgtg?actgacggtg?ttgagtgtct?caatacaaag?cgggttgatt 1620

ggtatgcagg?gatgtgatga?tgtgatcagg?tcagcgcttg?atttcaatcg?acgaggcagt 1680

ggagaacgaa?gatgtagata?gttttgattt?ctagtactac?tttgcggggc?ccctgttgaa 1740

tttgagtcat?tcttttgatt?gatcctccgg?aatcatatat?caagtaatgg?tgcagcgtag 1800

ggagtactcc?ataaggcaga?tgaagtacag?gtcttaaaac?ggtatgatcc?agtaagacaa 1860

agcggatctt?gtattccaaa?caatcacgtc?ggtaacatgg?actactactt?tcatctataa 1920

tgtttgtatg?ccagttgtgc?tgcagattat?taatttgtat?aggcattgta?caggtgcgct 1980

acattgttca?gcaagtaaac?gtgattttga?agtccccaag?aacacaaagc 2030

<210>45

<211>3080

<212>DNA

＜213〉aspergillus niger

<400>45

cttcggctgc?gtcatgattc?atgcgtcaaa?tcagtggact?cttagtaggt?ggatgtacag 60

cacttatttg?atacttcgag?tgtccgccaa?catccggccg?tctatattct?ataccaatcc 120

acattatgcc?caacgaaact?gtatgctgtg?atttaagcag?aagtgagcaa?agcactgtgt 180

cagctgatct?aatatactat?atgctccgat?taaacccaag?cagtctgcta?ccagggagac 240

taccaggcag?aaagtatctg?gctgcatttg?tcccagtctg?cacaggactc?gctatgttat 300

ggagcagaag?tattgtgcat?ggattccatc?tgtcccacta?gcatgactat?ctcaatatcc 360

accgggtaca?tgatttagcc?ggtatcactc?ccccgtccct?gtagcaaagg?cccgcgcaag 420

aggtgtttcc?agaaagggct?ccacgcttag?caccgttcag?caaattctgc?atcagccgcg 480

gggaaactgc?ggtttgttgc?caggtcgagg?tgcttaaata?ccaccgtctt?ctcgctcgtc 540

agtgctctgc?tcaggcgtcg?ctcagtcaag?ttcagttcaa?gactcaggat?atccgggcag 600

tcctccgcaa?ctatgcgctg?ctccctcatc?tcccttctag?gcctggcggc?catcccggcc 660

cttggaggct?gtcccttcgc?acacactgcg?aacatgggca?ttgataacat?ggtgaaagca 720

cacgctcaca?tgtcccgacc?gttgattgcc?tccaagagca?gcccctcaac?tgttcctacc 780

tcctctagca?ccccttctgt?cgggcagaaa?ggcgtgttca?tgatgaaccg?cattgctcct 840

ggcacatccg?agctctacat?tgccaacaca?gatggcagta?atgaacgccc?actcctctcc 900

aaccccgtct?acgagtacca?tgcctccttc?tccccggatg?tagaatggat?caccttcacc 960

agcgagcgca?atggtgacgg?taactctgac?atctaccgcg?tacggaccaa?cggctccgat 1020

ctccaggaat?tggttgccac?gcctgcagtg?gaagactccg?ttgttatctc?tcccaacggc 1080

cgcctggcag?cctacgtctc?caccgccaac?aacatgaagg?caaacatctg?gatccttgat 1140

cttcagaccg?gcgcgcagtg?gaacctcaca?aatacaccca?ccactgccgc?caactcctcc 1200

ctcatggaga?gctatctccg?tcctgcctgg?tctcctgatg?gcgaatggat?cgccttctct 1260

tcggaccgca?acacccaatg?ggacggacac?ggcgtaccga?ccttcctcgg?ccgcacgggc 1320

tgggagacga?cgcaagaact?ctctctctac?gccatccgtc?ccaatggctc?tgacttccgt 1380

cagatcatct?ccaagccata?ctactctctt?ggatctccga?aatggtcagc?agacggtaaa 1440

cgcatcgtct?actacgaaat?gacccgggaa?gacacctaca?acgcccatcg?tccagaaacc 1500

attaccacag?ccaactcgac?gatcatgtcc?gtagacttcg?agacaggcac?cgatgtgcgc 1560

gtggaagtcg?ccggctccgg?tgtcaagcaa?ttccctcagt?acctggacaa?gaacggcacc 1620

atcgcctaca?ccctcaaagg?cggcaccagc?gagggcttct?acacgaccgc?gggactctac 1680

gtcaacacga?cctcggcgac?cctcaggtcc?ccggcgtggt?ctcccgacgg?caagcaagta 1740

gtctacgaaa?agagcacctg?gagcatccgc?tcggggtaca?agcagctcta?cagctgggac 1800

agtgactggg?actaccgctt?cacggacgtc?ttccctcagg?tctcgcacca?ggagcgcgtc 1860

gccatcacac?agaagcagct?gggcaattcg?tccatcgtga?cgttgaacac?aaccggaggc 1920

gacttgcaac?tcgtctacga?ccccagcacg?gcggactttg?tcagcgatga?cgaaaccaca 1980

ggactgagcg?cttaccagcc?cagctggtca?ccctgcggcg?agtggctcgt?cttcggcgtc 2040

ggattctggt?tcgagacgag?agaagcctca?ggcggatgga?tcgtgcgggc?caccgccaac 2100

gggagctact?cggaggttct?cgtgaacagc?agctactcca?tcaccgagga?tggagccctg 2160

aacagcgggt?tcccgagttt?ctcgccggat?ggcaagaaag?tggtgtatcg?ggtttgggga 2220

gccgacactg?caacctacgg?caacgccagc?gagatcgggc?tgcgggtgct?ggacctcgag 2280

acgcgaaaga?caaccgtcct?aaccacagaa?tgggacaatc?tgccccagtt?ctctcccgat 2340

ggagagctca?tcctattcac?acgcaaaacc?agcacgtaca?attacgatgt?gtgcacgatc 2400

cggccggatg?ggacagatct?ccgcgtgttg?acgagcagcg?gtgctaatga?tgcgcatgcg 2460

gtctggtcgc?aggatggacg?gattatgtgg?tctaccggca?tgtatgggtt?ccggtttgag 2520

tgtgcgctgt?atggtgatac?gttccagccg?tatgggcagg?ttatgattat?ggatgcggat 2580

gggggaaata?agaagttgat?gaccaactcg?atgtgggaag?attcgatgcc?gttgttcttg 2640

ccgagggagg?tactttagtt?ggtggcggga?ggatgctctg?atttttagag?gatatgtgac 2700

gtttgatgta?tgataagaat?tacacaagta?gttttgatca?taacgcgttg?aagaggatga 2760

aaaaaaaccc?ccgttgttcg?acttgatctt?ggcgatccgg?tgtagggttg?ttggacgagc 2820

aggatgtact?gcttaattca?agcaagtttg?ctactccgat?tcagtatgga?ggggcaatta 2880

atgatgacga?tattctgcac?tttctactct?acctactact?tatctgtata?tgaatgagaa 2940

aaaaatagta?tgcaacgaat?atagatcgta?ttgacagaac?ctgtcagaga?gaatgacctc 3000

gatagtcgac?tccatagcat?ttgatctttt?actaacagcg?aaagattccg?gactaactat 3060

ccatatatag?tactcctaca 3080

<210>46

<211>3290

<212>DNA

＜213〉aspergillus niger

<400>46

ttccaacttc?ccatgaagtg?atgtagtagt?ctgctaataa?tacctcaaga?tagaatatag 60

tatttactac?ttatcgtcag?ctttgtcgaa?aattcaaagg?tttgtggaag?gccacctcat 120

ccgcacctgt?tgcctacagg?gatagcatcc?tgcgaggtgc?ttatccgctt?atcagcaaag 180

gtggcagggg?gggtaaggct?atcccgggtt?aggaaggccc?gcggcacgat?gcgatccttc 240

cagactagga?agctacctaa?tagggacagt?aagggaccaa?tctcaggact?aaaccgtgca 300

gcctactgaa?tctcaccact?cggacctgat?atttgccttc?ggcttgcagg?ggtatatcat 360

ctcaatatcc?tctagtggac?ggaaactagc?aactagtact?ttgagctgac?tcggccacta 420

taaatatgcg?gtcttatgct?cctcaacgac?aggcaactgc?tctgactacc?aaaatctgag 480

taggactagc?accgttgcga?gctcctcttc?acgataccga?atcttttata?agcaagtggt 540

ccaatatcca?ggtctaacgt?atccatacaa?tgcctccgga?tgcaaaatcg?cctggctacc 600

agcctggtat?ggcagtatta?ccatctaggc?cacatcctgc?caagggaaaa?gccattcgat 660

tcctcctttc?ccttgcattg?gtcgcgtttg?ctattgttca?attatgtggt?aatttccaca 720

aaaataggag?cgttgaacaa?cagcttcaga?gtcaaacact?tgatgatgag?tcctttaaat 780

gggaagatgt?gtgtaaatct?atcacgttgg?gatgtaacgt?tctaacgcac?tctattaggt 840

tactcctacc?aagcaactcg?tataccatcc?atgctttggt?gatcacgaat?gcgctcgctt 900

gtcgcttcca?atgaattgga?accgaactga?tggtgaaggg?tcaaaaattg?ccttggcggt 960

tatcaaactt?cctgccaagg?tacctgtcac?agatgcgcga?tatggtggtg?ccattcttct 1020

gaatccaggt?atgtagagcc?atattgctac?tcttcagcgg?ctgtgctgag?tttcaaatag 1080

gtggtcctgg?tggatccgga?gtgagcatgg?tctttagata?cgggaaagct?atccagacca 1140

tcgtcgactc?cccagaatca?ccaagtgcag?attcagcgag?cggaaagtat?ttcgatgttg 1200

ttagctttga?tccaagaggg?gtcaacaaca?caacacctaa?tttttcctgc?ttccctgacc 1260

ccgcgacgag?gaaagcgtgg?ttactgcagt?cagaggcaga?gggtctactt?gggagttctg 1320

aaggagtctt?cgatactcga?tgggcaaggt?acgaagcttt?tggtatgagc?tgcaatcaac 1380

aaggagtcac?agcgtcaaag?gatggagaat?ggataggaaa?attcatgaat?acggcccccg 1440

tggtggcgga?tatggttgaa?ctcgtagagc?gccatggaga?gtggcgtgaa?cgggaaacag 1500

agcggctact?ttcgacagct?ccgaacactt?tcccagttgg?aacaaacgtt?gacgccgaga 1560

ggataaggct?gcacaaccgt?tggaaaaaag?gggaggagaa?gctgctatac?tggggctttt 1620

cctatgggac?aatcctgggt?tccacgtttg?cggctatgca?gcctcatcgc?ataaaccgtg 1680

ctgtcataga?cggagtctgc?aacgctgatg?attattacgc?cggcaactgg?cttaccaatt 1740

tacaagattc?ggatgcagca?ttcaataaat?ttttcgagta?ctgctacaca?gctggcccat 1800

cagcgtgtcc?gtttgcgctc?ggcggagatc?ccgaagatct?caagtctcgt?tatgagcaga 1860

ttttgaccaa?tcttacatcg?agccctattg?ctgtgtctcc?ttctggaaat?aggggcccag 1920

agataataac?ctatagtgat?gtgaagtcat?tggtcgtgca?agctctctat?gtgcctttga 1980

aattattcga?tttggtggct?aggctattag?ctgagctcga?gcaaggtaac?ggctcttcat 2040

tcgctgactt?gaagtatgaa?gccaaacaat?ggccagtacc?gcctccatgc?gattcctcgt 2100

ccacacaata?caaagtacct?ggcgagagtg?atcaggaggc?cgggaggaat?atcctatgta 2160

cagatggtcc?aggcctcgac?ggaactgcca?aggaggattt?ccggagctac?tggaatatgc 2220

tccggggaca?aagtaaggcg?gttggagatt?tctgggccga?ggttcgcatg?tcgtgtgtca 2280

aactggagac?gcgacctgag?tggcgctatg?atggtaggtc?cgaccaacac?catattatac 2340

acacaatata?agctgacagg?tatgcgtatc?caagggccct?tcgcaggcaa?tacatcgcac 2400

ccattgctgt?ttatcgggaa?tacttatgat?ccagtaacgc?cgctacggaa?gtaagctttg 2460

ttcacctgat?cgtagcagac?tttgaagtac?agactgacgc?ataggcagtg?ctcatacgat 2520

ggcgcgtgga?tttcctgagt?caatcgttct?agagcagaac?tctgtcggag?tgagtacctt 2580

atctgttccc?caacccatat?gcaagatgac?taactgttca?atgtcgcagc?attgcacact 2640

gagtggccca?tccttgtgta?cagcgaaagc?gatacgccag?tatttccaga?ccggagagtt 2700

acctgacccc?ggaactgttt?gccaggtaga?ggagcttccc?tttcgtcttg?ccggatatga 2760

gagaagtcag?gtcatgtcgc?caggtgacac?agaattgatg?tccgccttgc?attcgctgag 2820

cgagttccgc?catctgctag?gcgcgtgaaa?aaggttaaat?aagtgtcgcc?agcgagcgct 2880

gcagttatta?atcctttaca?tcataggagc?tggctagaat?gttaacgatg?cagtttgccc 2940

ctgtatctcg?accaagagca?tgaataacac?attatatcag?caaatgttat?tgagcaaatc 3000

aattattcac?ctgcactcaa?ttcaatttaa?gatctgttgc?ctagactacc?agaattatta 3060

aggcgacctt?atcgaagcat?ttgcagacca?acccctgtcc?atcgtcaggg?gcaagacaga 3120

catttcttct?agaacccgga?tgatgtataa?tcgcggtggg?gaagatcatc?ctaaaccatt 3180

gccccagact?cgcctacctt?aggtaatcca?tacaaatgcc?ggggaaatca?ctgaggtgtt 3240

gttcatcacg?gcgttgaatc?ggcttctgcg?gtattaccac?cataactgta 3290

<210>47

<211>3080

<212>DNA

＜213〉aspergillus niger

<400>47

gaaaatcccg?accgttaggc?tacctaatct?gctgtcttta?cccccgttag?cccacttacc 60

cagctttagt?ctctcgaagg?aaaagaaaac?aaagtaatcg?ggagcctata?atattcggcc 120

agaagtgggg?caatcacttc?aaccgttcat?ctgttattga?gttatcaagt?ctgtcaagtt 180

cgtcggagct?ggataaggta?gtcgccatcc?ccatttccgc?tgtgcaatgc?cgcagcgccg 240

aagtgtctgc?agctagctct?attttgttca?gctacgtatc?tgtctcttaa?ccaaatggat 300

gtcgccattc?gcccactaac?aacgccattc?cccacggccg?acggactccg?gcgcaagggc 360

ctgataagcg?ttagctccga?ctccgagtta?gtcatgcggc?ttattacgcg?cccttctccc 420

ctcacgatgt?tgatgctttt?tctggtatct?ctggcataac?tggcaaagat?caggctgtgg 480

tcataagagt?atcattgcct?tcagcttctt?cgccatgttg?agtagtctgc?tgcttggggg 540

tcttctgggt?ctagcgaccg?ctcaatttcc?tcccgagccg?gaaggcatca?ctgtgctcaa 600

gtccaagttg?catgagaatg?tgactatttc?tttcaaagag?gtgtgtgcag?agtatctaga 660

aatagctttt?atgctcgatg?ccgtgctgat?tgtcagcctg?gaatttgcga?aactacgccg 720

ggtgtccgat?cttattcggg?ctatgtacac?cttccccccg?gtttcctttc?cgacgggaca 780

ggagaagtgc?aggattatcc?tatcaacacg?taagccaatc?tcgaaacatt?ggaggatgag 840

caattactga?gcctcaacca?gcttcttttg?gtttttcgaa?gcccgcaaag?atcccagcaa 900

tgcgcctctg?gccatctggc?tcaatggcgg?tccgggtggc?tcgtcgctca?tggggctcct 960

tgaagaatta?ggtccttgtt?ccattgcatc?agactccaag?accacagtcc?tcaatccttg 1020

gagttggaac?aatgaagtca?atcttctatt?ccttgaccag?ccaactcaag?tcggcttctc 1080

atacgatgtc?ccaacaaatg?gcactttggt?tcggactgcg?gacggcgaag?aagagatagt 1140

ttccggtgat?ttctccattg?atgttcccca?gtccaacttc?acccatcatg?ttggtacctt 1200

tgcaagccag?aagcttgcac?agacagctaa?tgggactgca?ttcgcggctc?acgctctatg 1260

gcatttcgcg?caaacctggt?ttttcgagtt?cccacactac?aagccaaacg?atgatcgtgt 1320

cagtctctgg?gctgaaagtt?acggaggcca?ttatggtcca?ggcatctttc?ggttcttcca 1380

acagcagaat?gacaaaatcg?cagaggggac?tgcagaagac?ggtgcacagt?atttgcatct 1440

cgacacgctt?ggcattgtga?acggcttgat?ggatatggtg?atccaagaag?aggcttacat 1500

tacttggcca?tacaataacg?taaggctcgc?cccttcttca?ttcaactcgc?gtaatgccta 1560

attcagttca?gacctacggc?ctcgaaatct?tcgataaacc?cctctacgaa?gaactgatgt 1620

ataactggac?gcatccagga?ggctttcgcg?atcaggccct?cgcctgcgaa?gcggctttga 1680

aagaacgcga?ttccggcttg?cctcactcag?ggaagaatat?ctctgaaatt?tgcggaggcc 1740

ttgcactaga?atggggagat?ggccccatca?cctactacca?caccttcaat?cgcgggtggt 1800

acgacatcgc?ccatcctaag?aacgacccat?tccctgccaa?gcacatgctc?ggatatttga 1860

cgcaggagtc?cgtccttgcc?gctcttgggg?taccagtcaa?tttcacatcg?tcttcgagtg 1920

ccgtggctac?acagttcata?aaaacctttg?atatcgtcca?cggcggcttc?ctggatgcaa 1980

ttggctacct?cctcgacagt?ggtgtaaaag?tacacatgat?gtacggagat?cgtgattacg 2040

cctgcaattg?ggtcgggggc?gaaaaagcca?gccttgcagt?tccgtattcc?cgtatcaccg 2100

aatttgccga?cacgggatac?tccccactcc?ttacgcccga?cgggatcagc?ggcatgaccc 2160

gccagctggg?caactacagc?ttcactcgcg?tcttccaagc?cgggcatgag?gtcccctcct 2220

accagcctgt?cgcggcgtat?gagatcttca?tgcgggcgac?attcaacaaa?gatatcccta 2280

ctggcctctt?ggctgttgat?gacgaattcc?agtcggttgg?acctaaggat?acgtggcata 2340

tcaagaatat?ccctcctatt?atgccaaagc?cgcagtgcta?tgttctaagt?cccggcacgt 2400

gtaccccgga?ggtttgggag?accgttttga?acggatccgc?gacggtaaag?gattggtatg 2460

tcgtggatga?tagcgcgggt?gttgaggacc?acgaggggtt?cagcattctt?ggaggggatg 2520

agttgtagta?gcttagaagt?ctaccggaaa?gccttgctag?ctcttaaaat?acgacacaaa 2580

tagttgagcg?acatagtcta?ctctctcact?tacgaacacg?tgatgaatct?ctccagcaat 2640

aaagtggacc?aatcaattgg?aggaaaatat?atccacgatg?gtcggacggg?ccaacgaaaa 2700

gggccaacta?gcgcccctct?gaggatgatg?gtctagtctg?caggatatga?gattgttcac 2760

atcttatggg?tcttcaactg?cacaaattag?gcggaggtcg?cctcaccgaa?agagacgtaa 2820

gctgcttttt?ggtggattca?ttattagccg?ttatgccatt?tactaaaaca?ccgcagttcc 2880

agatcgcgta?gatggcctca?aagtgttcgc?cagatttgat?tgagggctac?ttcgcccaag 2940

cctatggcct?gtagatgcca?atttcttcaa?tacaaagata?ggcgaaacat?gttgaaaatc 3000

catcatattc?aattaaattt?caaaacatag?cgagacatta?agtattacat?atatttcaat 3060

gaatagagac?ttcttgcaca 3080

<210>48

<211>2520

<212>DNA

＜213〉aspergillus niger

<400>48

tctcgcctgc?ctcgaccttc?ctgctatggc?aattctttcc?ccaagctgag?cggttccatc 60

cgcttcccga?ccgccaaact?tatcaacccg?agcgatacgg?agaggggaaa?ggctatcacg 120

gcaatgattg?cgcaactctg?cgataagata?accccatgtt?ttgacgtagc?ctcggccctg 180

tcttctcctc?attattatcc?tccgattccc?ccagggttcc?tgtcttctgc?agaaagtgct 240

ctcggaatcg?aagatgacca?ggtttcaatt?gcttcccctt?gtcgcagggc?tgcttgcccc 300

ttcaattgca?gcccttagca?tcccttcccc?gcagcagatc?ctcgattctc?tcactttcgg 360

agagcacacc?gacggctttt?gtccgctggc?acccaaggtt?gaggttcctg?acgatggttt 420

ctttccagct?ctcaagttcg?tagaagatgc?ctcgttcaag?tcgcgccaag?tcaatcgtct 480

ctccagggcg?gttcaagttc?cgaccgcaat?cgacgactac?atgaaggatc?cctacgacga 540

aaagttcgcc?ccattcctcg?acttccagaa?gctcctgcag?accctctttc?ccctcacgta 600

cgtccccagt?ccccgtcttc?atggctccat?ctaacgcaat?ccagccactc?ctacgcccgc 660

gtagatcaca?tcaaccgatt?tggtctcgtc?ttcaccctca?atggcacaga?tgactcgctc 720

aagcccctgc?tattcaccgc?gcaccaggac?gtcgtgccca?tcaacgaccc?tgccgactgg 780

acctatcccc?ccttcgatgg?ccactacgac?ggcgaatggc?tctggggccg?cggtgccagc 840

gactgcaaga?acgtcctgat?cggtctcatg?tccgttgttg?aagacctact?ctcccaaaag 900

tgggagccaa?cccgcacagt?cgtcctggcc?ttcggattcg?acgaagaatc?ccacggcttc 960

ctcggcgccg?gatccatcgc?caaattcctt?gagaagaaat?acggaccgga?cagcttcgaa 1020

tttatcctcg?acgaaggcgg?catgggcctc?gaagttctag?acgacaacaa?caacggcgtc 1080

gtctacgctc?tccccggcgt?tggcgaaaag?ggcagcatcg?acgttgtgct?cactctggcc 1140

gtaccaggcg?gccacagctc?cgtgccccct?ccacacacgg?gaatcggcat?catcgccgag 1200

atcatctatg?agctagaacg?ccaggacctc?ttcgtccccg?tcctagacac?tcaccacccg 1260

acccgcaaga?tgctcgaatg?ccaagtccgc?cactccccct?cgcaagtcga?accgtggctc 1320

gcctccgccc?tccaatcaag?cgactacatc?tccctagcag?agaaactggc?ctcctcgcgc 1380

ggcgacaagt?tccgcttcat?cctccaaacc?tcccaagcag?cggacatcat?caacggcggc 1440

gtcaaatcca?acgctctccc?cgagaaaatc?aacgccctcg?tcaactaccg?catcgctctg 1500

caccaaaccc?cagacgatat?caagaaccgc?gctgtggaga?tcatctctcc?catcgtcaag 1560

aaatataacc?tctccctcac?ggccttcccg?gaaagcgaca?ccgttgaccc?ctccctcaac 1620

aaccacctca?cccttactac?cctcagcggc?gccctcagtc?ccgccccggt?cagcccaacg 1680

gacatcgaca?ccgacgccgt?ctgggcccgt?ttctcgggcg?tcactcgctc?ggtcttcgaa 1740

tctgtcccta?gtctcgaggg?cagaaaggtc?gtcgtgagcg?gcgacatcat?gaccgggaat 1800

acggatacga?gattctactg?ggctttgtcg?aggaatattt?acaggtggag?tccgtcgagg 1860

gcgggtaaag?cgctgaatat?tcatactgtt?gatgagagga?tcgatattga?tattcatctt 1920

gaggcgatga?tgctgtatta?cggtatgcat?ctctcctttt?atggatactc?ctatcagact 1980

aacttgtatc?tctagatctt?attcgctctt?tcgatggacg?gaccgattca?tctgtcattt 2040

ctgctgcgtc?ggcagctgct?gatgatgaac?ttgctcacga?cgtgctgtga?gtctgtagga 2100

actctcctac?tctttagggt?ggctcaggat?gaatgatggt?tatgaatcat?gtggtctact 2160

gcgccaaaag?ttcatgtctt?tcctggagtt?caacgagaac?cagatctata?tttccacgaa 2220

tagctccagt?atatagccaa?atgcaaccac?gtatttgtct?atcgatctga?cagtctaatt 2280

ctttacctta?tactaggggt?aagaaagggt?aagaaaataa?aataatattt?gcagcggcct 2340

atgaagataa?ttatgtactc?gacgttgccc?aatgcactgt?agcccctctc?cctgtaacag 2400

gagtagccat?aaaagcaaaa?cgaaagtggt?aagaagacgg?gggcaatatc?cctgctttag 2460

caatatacaa?caatagtaac?aatcgagagc?tcaatatgaa?tgtataacga?gtgacctgca 2520

<210>49

<211>2730

<212>DNA

＜213〉aspergillus niger

<400>49

tttagttgtg?atctcaatcg?caaatggaca?ggagatgata?ttcatgtcat?aatcagagtg 60

ccatgtcagc?gaccatgtct?tgccaagagt?catcatttcc?ggtaaaaccg?acggaggtaa 120

aaccccggtc?attcactagt?tcgagggagg?gaatagtctc?gcaggtctct?tcttctttct 180

tcctcggggt?ggcggatgga?tcacttcttc?atttgatggt?tgcctgccaa?cgacccactt 240

gactaggttt?cccccttgat?gggattcgtt?ggttttacca?gacagagcct?cttcacagcc 300

cagcgcgtgt?tccgcaaaag?caacagccca?agggtctgaa?ggctgacatt?ggagcgggtc 360

tgacgcagga?tggcttgctg?ttaaaaaaac?tcaggaacag?gccaagacga?agcgaccgcg 420

caaggctgag?cagttctccg?atttccccgt?gcccctcctg?cgcctcccat?gggcgacttt 480

tgatcccaga?tccatcgttt?ctgcaccgac?catataagaa?gctccgcacc?cccaaactcc 540

cctggcaagt?tcctgccccc?aaattacgtg?agacagggaa?ccatcacagc?aacaccatga 600

agagcaccac?tcttctttcc?ttggcctggg?ctgcccagtc?cgcctattcc?ctctctatcc 660

acgagcgcga?tgaacccgct?actcttcagt?tcaactttga?acgtcgtcag?atcgccgacc 720

ggtcccgtcg?gaagcgatcg?acggcctcgg?ccgacctcgt?taacctggta?tgttcccatc 780

ccgagtctca?aatcagggga?attatgacgg?tcgctaatgc?aggtttctag?gctacgaatc 840

ttggctacac?gatgaacctc?acactcggca?ctcccggcca?ggaagtcagt?gtgacgttgg 900

acaccggcag?cagcgatctc?tgggtcaatg?gggccaactc?gtccgtctgc?ccctgtaccg 960

attacggctc?ttacaactca?agcgcttctt?ccacctacac?cttcgtgaac?gatgagtttt 1020

atatccagta?tgtcgacggc?agtgaagcca?caggcgacta?tgtcaacgat?actctaaagt 1080

tctccaatgt?gactttgacg?aactttcaat?ttgccgtcgc?atatgacggc?gactccgagg 1140

gtaagtcttc?gctattccct?cactttcatt?ttacactttg?ctaacggttt?taccatgcag 1200

agggggtcct?cggtatcgga?tacgccagca?atgaagccag?ccaggccacc?gtcggtggtg 1260

gtgaatacac?caacttcccc?gaagccctcg?tcgatcaagg?cgcgatcaac?tggccggcct 1320

acagtctatg?gctcgatgac?ctcgacgaag?gaaaaggcac?cattctgttc?ggcggagtca 1380

acaccgccaa?gtactacggc?agcctgcaga?ccctgcctat?cgtctccatc?gaagacatgt 1440

acgtcgagtt?cgcggtcaac?ctgacggccg?tgcaccttga?gaagaacggc?aactccgtct 1500

cggtcaacaa?cagcgccacg?caattcccca?tccccgccgt?gctggacagc?ggcacggccc 1560

tgacctacat?cccgacctcc?gccgcagcca?gcatctacga?ggccgtcggt?gcccaatacc 1620

tgagcgagta?cgggtacgga?gtgatcgagt?gcgacgtcaa?ggacgaagac?ttcaccttcc 1680

tgttcgactt?tggatccttc?aacatgagcg?ttgacatcag?cgagatgatc?ctcgaggcca 1740

gttccgacat?gaccgacatg?aacgtttgta?cgtttggcct?cgcagtgatc?gaaaatgagg 1800

ccctgctggg?cgataccttc?ctgcgcagcg?catacgtcgt?ctacgatctc?ggaaacaacg 1860

agatctccct?ggccaaggcc?aacttcaacc?ccggcgagga?ccacgtcctg?gagatcggca 1920

ccggatcgga?tgccgtgccc?aaggcgacgg?gggcgacggc?gaccggcgcg?gcagccacat 1980

ccacggcctc?gagcgacaag?tcggacaagg?agagttcggc?tacagtgccg?cgcagccaga 2040

ttgtctcgct?ggtggcggga?gtcttggtcg?gtgttttctt?ggttctgtaa?atatagagat 2100

ttcacgttgc?atgttgatga?tacataccat?agatttgctt?ctaattgccc?cttacatctc 2160

gaaatgtttt?ctgtttttag?cttgatatcc?catcgcttca?ggtttcagat?atggaaatga 2220

aatgaaaatc?ggaatgcaag?atctgatcga?atagttgttt?cactgccgcg?tggctggtcc 2280

gatcggcacg?aagccagtaa?tcgacaacca?atcatacatt?gtgatttcct?gtcaacctcc 2340

ttgcgactac?tactaatttt?atcatatcat?gccgttgtga?cctatcctac?tcggtgtccc 2400

cacaccgatg?atagccaccc?gcgaccggat?aatcggtaac?ctgccaagag?tcgaacggct 2460

ttcctgtgga?aagggtggct?gcaacctgtt?tgtttcagtt?tttgtgttgc?agaattgact 2520

atccttctga?cctgtgaatg?atactgggat?ggtgacgtag?tgaaaatgtt?ggtggagcga 2580

ttaaggtttg?tgcctgtcag?cctcgtcaag?tccatcatca?tgtcttttcc?ggactatcac 2640

ttcctctact?ctgaagtacc?gggactcccc?gccagtattc?gtttcgccaa?tcagagcatt 2700

cgatgtgaac?cggattgaag?agtgcccggt 2730

<210>50

<211>3231

<212>DNA

＜213〉aspergillus niger

<400>50

ccgacgcgac?ggcattagtt?cctcgatagc?gtgggaagac?catggagaga?ccggccgata 60

agctgcagag?gagctttctg?tgggctgacc?agtgcctatc?agcaaatgga?cacgcggtga 120

cacccccgca?atcgcaagac?cgcctcgaca?ccgtccatcc?aggcaccatc?gcaacggcaa 180

accagcctct?cctccaatca?aaccatcgat?gtgatattgc?tagcggcggt?gagaacagca 240

ggtcgaatgt?cgcaattaat?tctcgcatcg?tccctcactg?caaggggccc?actaccagag 300

ctctgcggcg?ttgttattta?aatccgttgt?tgggaccttt?cggtcatcag?ccactgtgtc 360

tggtcactat?cgcgctggtt?caacctcaac?atgttggtcc?gtcagcttgc?cctggctctg 420

gccattgcgg?ccttgtccga?tgccattccg?acatccatca?agcatgtcct?gcacgagaaa 480

cgtcacaagc?ccgcatccga?ctgggtgaag?ggtgcgcgcg?ttgagagcga?tgcggtcctg 540

cctatgcgca?ttggccttgc?ccagaacaac?ttggacaagg?gctatgactt?cctgatggaa 600

gtgtgagtca?aaatctacct?tttttatgct?atatgcctag?ttttaggatc?gcaatggatg 660

acctcgaaac?atgctgacat?gagattggcg?cagatcggac?cccaagtctt?ccaaatacgg 720

ccagtactgg?tcggcagacg?aggtgcacga?catcttttcg?ccatccgagg?aggctgttga 780

ggcagtgaga?gaatggcttg?tcgcctctgg?tatccatccg?tcgcgggtgg?tgcactccga 840

caacaagggc?tggctcgcgt?tcgacgccta?cgcccatgaa?gccgagaggc?tgttcatgac 900

ggaattccac?gagcacgaga?gcgaccgaag?tgctaagatc?agggttggat?gcgaccagta 960

agaagattct?tctatcacct?tccatgagta?gctattaatc?ggaatctaga?taccacgtcc 1020

ccgaacacat?ccagaagcac?atcgactaca?ttacccctgg?agtgaagctc?acccaggtcg 1080

tgaagaggac?caacaaagtc?aagcgtgctt?cccaactagc?tcactcttcc?aaggccaagt 1140

ctgctgccca?aggtccgcag?ccactcccca?acaaggccaa?gttcctgcct?gaagacctcc 1200

gcggctgcgg?ttacaacatc?accccctcgt?gtatcaaggc?cttgtatcag?atcccagacg 1260

ctaagacggc?gaccccgaac?aacagcctgg?gtctgtacga?gcagggtgac?tactttgcca 1320

agtccgacct?cgacctcttc?tataaggagt?atgcgccgtg?ggttccccag?ggtacctatc 1380

ccatcccagc?cctgattgat?ggcgccaatt?actcggttcc?ttcctacagc?tccctgaaca 1440

cgggtgaatc?cgacattgac?attgacatgg?cgtgagtcat?ttctgcacct?tgtcatcaga 1500

cccctactga?cgttttgaag?ctactccctg?ctctaccctc?agcaggtgac?cctctaccag 1560

gttgacgacc?agctctacga?accagtcgag?gtcgacacaa?ccaatctgtt?caacaccttc 1620

ctcgacgctc?tcgatggcgt?gagtacagac?ctcgttctca?gtcttaccca?gctaacaccc 1680

ctagtcctac?tgcacctaca?gcgcctacgg?cgagaccggc?gatgacccgt?cgatcgaccc 1740

cgtatacccc?gacacccgcc?ccggcggcta?caaaggtacc?tacctacacc?acctcttccc 1800

catacaatcc?aacctaacac?accaacagga?aagctccagt?gcggcgtcta?taagcccact 1860

aacgtaatca?gcgcctccta?cggccaatcc?gaagccgacc?tccccgtcag?ctacaccaag 1920

cgccaatgca?atgagttcat?gaagctcggt?ctacagggac?actccatcct?cttcgcgtct 1980

ggcgactacg?gcgtcgcgtc?tttcgccggc?gacggtgacg?agaacggctg?tctcggccca 2040

gagggcaaga?tcttcaaccc?ccagtacccc?tccaactgcc?cctacgtcac?ctccgttgga 2100

ggtaccatgc?tgtacggcta?ccagaccgtc?aacgacagcg?agagcgtcat?gcacgttaac 2160

cttggcggaa?ccgcaagtaa?cttcagcact?tctggtggct?tctcgaatta?cttcccccaa 2220

ccggcatatc?agtttgctgc?tgtggagcaa?tacttccagt?ctgcgaacct?gtcgtatccg 2280

tattactcgg?agtttgaggt?cgatgttaac?acgaccaagg?gtctctacaa?taggcttggt 2340

cgtgcttatc?cggatgtctc?ggcgaatgga?gcgcatttcc?gcgcttatat?ggatggatac 2400

gattatcatt?ggtatggatc?gagtttggcg?tcgcctttgt?tcgcgtcggt?tcttactttg 2460

gtgagctttg?tcacccccca?ttactaatta?ttgacacatg?gctgaccgac?ttagctcaac 2520

gaggaacgct?tcgctatcgg?caagggcccc?gtgggattcg?tgaatcccgt?gctttatgct 2580

tatccgcaag?tgctgaacga?tatcactaat?ggtactaatg?ctgggtgtgg?aacttatggg 2640

tttagtgcta?ttgaggggta?agtgctcagt?acttggttct?gtcaggaggg?gtgtgctaat 2700

tgatgactat?agatgggatc?ccgctagtgg?tttgggtacg?cctaactacc?cattgatgaa 2760

ggagctgttc?ctctctttgc?cttaggattg?aacggtgctg?tgtcagaggg?tgataggtgg 2820

tcaagctgtg?tatatatgtc?tgatggggaa?atatttacga?tcataggata?atgtgtcgac 2880

gagcatgaat?ggccaattat?ctcgcctgtc?accgtgaata?aggtcaaatg?tagatcggtt 2940

taatagttca?actacagaga?attcttggat?attgtcaaat?gttgactatt?cgctgtctct 3000

ttatcgtcta?atgtataata?tcatcaaata?acctaaccca?agggatatca?aaaacataag 3060

aaaataataa?accggtacta?tgtgtcgaaa?aaaggaatgt?ttgtgaattt?tttaaaaccg 3120

ttcatacctc?ccgtccatgt?ccaccaaatg?cacagcgctg?gtcgaatccc?tctccgaccg 3180

tgtcgtatag?tcaacggaga?tattcactga?cttggaaatc?ttgctgttgg?t 3231

<210>51

<211>2660

<212>DNA

＜213〉aspergillus niger

<400>51

tcctgagcaa?gcagctaccg?gtaatctgag?acctaatcct?ggtaagtgga?tcgagttcat 60

ttacctcatc?atctaacctc?gcttcatgat?tcgcaggcgc?tttccgcgac?tagtcgatgc 120

cagtatccat?ttgcctgcag?atccgatgct?tccaccgacc?accgttactt?ctctccagac 180

ccccatccgg?ctttccgacg?gcctcctctg?catcccctca?caaagcaagc?ggagacctgc 240

ctgaataggg?agagtttttt?caatgagccc?acacgcagtt?acctcgcttc?cgcatgagat 300

gggtccattc?ttgacaatgc?tgcctaatgc?gcagaagggc?cgtcatgcgc?ctggagaact 360

acataaatag?ggaccacgca?tgccccgacg?atgtatgaat?tgttcaaact?tctccgccca 420

agtgagttct?cttttcattc?cattccgaag?ggagaatcac?cagtatgcgg?gttaccacgg 480

caattgcttc?attactactg?gtcggctcgg?ccaccagtct?ccaaaatcct?catcgtcggg 540

ctgttccgcc?ccctctctcg?catcgcagcg?tagcgtctcg?ctccgtgccc?gttgagcgcc 600

gaaccaccga?ctttgagtat?ttgactaaca?agactgcaag?tgcgtgattc?cgttttttaa 660

ctaccgcatt?tatcgttcta?agatcaattg?caggattcct?ggtcaatggc?acaagcatcc 720

ccgaagtcga?tttcgacgtc?ggcgagtcct?acgccggcct?tctccccaat?acgcccactg 780

gcaattctag?cctattcttc?tggttcttcc?cctcgcaaaa?tccagaggcc?agcgatgagg 840

ttagtggtcg?ctctgttttt?tccggtcatg?cgtcagccag?ctaacaatta?acaaagatca 900

ccatctggct?caacggcggc?cccggatgta?gctccctaga?cggcctgctt?caagagaacg 960

gcccattcct?ctggcagcct?ggcacttaca?agcccgttcc?taatccatac?tcatggacca 1020

acctcaccaa?tgtggtttac?atcgaccaac?ccgccggcac?aggcttctcc?ccgggcccct 1080

cgaccgtaaa?taacgaggaa?gacgtggctg?cccagttcaa?cagctggttc?aagcacttcg 1140

tcgacacctt?cgacctgcac?ggccgcaagg?tctacatcac?cggtgaaagc?tacgcgggca 1200

tgtacgtccc?ctacattgcc?gatgccatgc?tgaacgagga?ggatacaacc?tacttcaact 1260

tgaagggtat?ccagatcaac?gacccgtcca?tcaacagcga?ctcggtcatg?atgtactgta 1320

tgtttccctt?catatacctc?cacctccacc?accaccacca?ctaacaacat?cacccaccag 1380

cccccgccgt?ccgccatctg?aaccactaca?acaacatctt?ccagctaaac?tccactttcc 1440

tctcctacat?caacgccaaa?gccgacaagt?gcggctacaa?cgccttcctc?gacaaagcca 1500

tcacctaccc?accccccagt?cccttcccca?ccgcccctga?aatcaccgaa?gactgccaag 1560

tctgggacga?agtcgtcatg?gccgcctacg?acatcaaccc?ctgcttcaat?tactaccacc 1620

tgatcgactt?ctgcccctac?ctctgggacg?tgctcggctt?cccctccctc?gcctccggcc 1680

caaacaacta?cttcaaccgc?tccgacgtcc?agaagatcct?gcacgtccct?ccaacggact 1740

actccgtgtg?ctcggagacc?gtcatcttcg?cgaacggcga?cggcagcgac?cccagctcct 1800

ggggtcccct?acccagcgtc?atcgaacgca?ctaacaacac?tatcatcggc?cacggctggc 1860

tcgattacct?cctcttcttg?aacggctcgc?tcgccacaat?ccagaacatg?acctggaacg 1920

gtaagcaagg?gttccagcgt?cctcccgtgg?aaccgctctt?cgtcccttac?cattatggtc 1980

tggctgagct?gtactggggc?gatgagcctg?acccgtataa?ccttgatgct?ggcgctggat 2040

acctgggtac?agcgcatacc?gagcgcgggt?tgactttcag?ctcggtgtat?ttgtctggtc 2100

atggtaagtt?tattatatcc?ccttggaagc?ggtatgatga?acgttagaga?gtgctgactg 2160

ttgcttcctc?ctcgtgatag?aaatcccgca?gtatgttcct?ggtgcggctt?accgccagtt 2220

ggagttcctg?ctcggtagga?ttagtagtct?ttcggcgaag?gggaactata?cctcttgatt 2280

tcagcggatg?acgacaaaaa?gatgatgagg?aagatgattg?gatgctttca?gatgggaatg 2340

cgtgcatagg?agatgagatg?agatgatgta?tcattgaggt?gtgatgactt?gtacatatgt 2400

agatcggtag?taaaagggat?actataggat?atggtatttg?atgtatcatt?tatgtacacc 2460

acgtggattc?aattgagggg?agcttcaatt?cctggttatt?acttatcata?tttccaacat 2520

gtccgcgtat?aaccggtaac?aactaacggc?ttcatgtttg?tcaagtgact?gtcttggtac 2580

aatactactg?tttctatatt?ctactgttgg?taacttaatc?tggacatatt?ctatatccac 2640

gtacagattc?tcgctagatt 2660

<210>52

<211>3150

<212>DNA

＜213〉aspergillus niger

<400>52

ccaatcaacc?acggatgatt?gggttcaggt?cctggaggca?cggagtcgga?ccaagacact 60

aaggtacagc?actagcgaga?caatgtgtta?tcgctattat?tggcaaaatg?gccgcgggat 120

ctcttatgca?gggttcggct?ccatcctccc?ccctcttcct?atccagtcaa?tccgcctcgg 180

ttattgaagg?agatcctgag?ctgtttaact?gacgcctcac?cgatcaggcc?ggaaatggtg 240

gcgggataca?acatcgtttc?cacacaatag?tgcttgtctc?ctgcgatctg?catggcatgc 300

taatctccgc?cagcatgtat?cttctatcca?ctggatatga?attttcctcc?cctcacacca 360

tgtgggcctg?ggggttttcc?ctcaaacttt?gtcgctcatg?taacgatgta?tataaagccc 420

tgaggatggc?atcccccacc?catcggtctt?ttgctgaccg?ttctccttga?agaaattctc 480

gagtggcttg?tggtgcatgt?atagatttaa?tcttcgaggg?ttattaacta?ggtatagctg 540

tgactaagtc?tgtccttgca?ttgaacaaca?caccatgcgt?ggctctcggt?tggtgctctt 600

gttgcccctg?gctgcactta?gttgtgctat?gcccgagaat?gaatggtcat?ctacgataag 660

aaggcagtta?ccaaaagcgt?ccactggcgt?caaatcgata?aaaaccccaa?acaatgtcac 720

tatcaggtat?aaagaaccag?gaaccgaagg?aatttgtgag?acaacacctg?gggtcaaatc 780

atactccgga?tatgtcgatc?tttcgccaga?gtcgcatact?ttcttttggt?ttttcgagtc 840

acgccgtgac?cccgaaaatg?atccagtgac?tctgtggctg?aatggtggcc?ctggaagcga 900

ttccttgatt?gggctttttg?aaggttggcc?aaatatcctg?acggaaaaga?taaaattcag 960

cttgcatgtt?ctgacgcctt?cacaacagag?ttgggtccgt?gtcacatcac?accagagtac 1020

gaatcaatca?tcaatcagta?ctcctggaac?gaggtcacca?atcttctttt?cttgtctcag 1080

cccctcggtg?tgggtatgga?atattgctgc?cttcatacat?cctgagtaca?ttgcttacgg 1140

tcttatctgc?gaagggttct?cttacagtga?aaccgaggcc?gggtccttga?atccatttac 1200

tggagccgtc?gagaacgcct?cctttgctgg?agttcagggt?cgatacccag?ttattgatgc 1260

cactatcatc?ggtaagttgt?ccggtttgac?tctcacctag?cattctcctc?aatgtcctac 1320

tttacagaca?cgaccgatat?cgctgcacgc?gcaacctggg?aggtgcttca?gggcttcctc 1380

agtggcctgt?cgcagctaga?ttccgaagtc?aagtccaagg?agttcaacct?gtggacagag 1440

agttacggag?ggtgagtgca?actttcatac?cagaccgacg?taagctgact?tgatcaagac 1500

actatggacc?agcggtaggt?tgtcttttct?ggttgcacac?atattgatct?aatgaccgaa 1560

gttcttcaat?catttctacg?agcaaaattc?gaagatcgct?agcggggaag?tcaatggcgt 1620

ccaactgaat?tttaactccc?tcgggattat?caacggcatc?attgatgccg?cgattcaggt 1680

acttagaaat?gcagctcgcg?cagaggctgc?ggcctagaag?gacatcgcta?aagtaattaa 1740

taggcagact?actacgcaga?ctttgccgtt?aataatacat?atggaatcaa?agctgtaagt 1800

ttaaatacac?gtacatcgtg?gatttaagat?caaccgtgct?catgcttgct?aggtcaatga 1860

cacagtgtac?aactatatga?agttcgccaa?cacgatgcca?aatggatgcc?aggatcaggt 1920

tgcttcgtgt?aaattgacca?ataggacctc?gctttctgat?tatgctatat?gtacagaagc 1980

agccaatatg?tgcagggaca?atgtcggtga?gtggttctac?tgtttctctg?caggggtgca 2040

atgatgaagg?actttgctaa?gctgtcatgt?acagaagggc?cttactacca?gtttggcggc 2100

cgtggcgtgt?atgatattcg?gcacccctac?aatgtaagtg?gcaaggataa?ggattgtact 2160

ttccgaacag?ggacactgct?catatgtcaa?cgtaggaccc?gaccccgccg?tcctactttg 2220

ttgactacct?caagaaagac?tcagtcatgg?atgctatcgg?cgtggacatt?aactacaccg 2280

agtccagcgg?cgaagtatat?tatgcattcc?agcagaccgg?cgactttgta?tggccgaatt 2340

tcattgagga?cctcgaagag?atcctccaac?tccccgtacg?cgtgtcgttg?atctacggcg 2400

atgccgacta?tatctgtaac?tggttcggcg?gtcaggccat?ctcactcgca?gttaactacc 2460

cccatgcagc?tcagttccgt?gcagcgggat?acacacccat?gacagtagat?ggggtcgaat 2520

acggtgagac?tcgcgagtat?ggcaactttt?cgttcacccg?cgtatatcag?gctgggcacg 2580

aggttccata?ctatcaaccg?atcgcagcgt?tgcagctgtt?caaccgtact?ttatttggat 2640

gggatattgc?agcgggtaca?actcagattt?ggcccgaata?tagcaccaac?gggacatcgc 2700

aggctacaca?cacggagtcg?ttcgtgccac?tgtccacggc?gtcgagtacc?accgtcaatt 2760

aggattgggg?gaaatttttc?cctctttggt?atggctaatg?ctgtgttatc?cattccgata 2820

cttgtccata?acctaaaaga?gtggcacgga?agctttccta?gacatgcttg?ctctagtcaa 2880

cttttatcct?accactgtgt?ggtcctacca?taatgtcatc?ctaaacttat?caggtgtctt 2940

acatcatttt?gcagtgacca?taaaagtcat?gtcatattaa?gtccattacg?gtagttcgta 3000

acttgctgat?ggcttacgta?attgtgcctc?agcaggatgt?cgtgatacgc?tccaaaaccc 3060

aatctgatca?tggatatcca?gcggaataac?acaaaagaaa?aaataaacat?aaccaaccac 3120

caaaatgaca?gaggctaatg?cctggacaac 3150

<210>53

<211>3221

<212>DNA

＜213〉aspergillus niger

<400>53

ccatcagctc?ttgttgttat?gttttatgtc?atagtgtaat?gctagcctta?gcgtgcgtgg 60

tgtttgaatt?tcagattggg?catggatacc?tgggcgtctc?gggatggata?ctgggacgtg 120

gtaagatgct?tggaagcgtt?gattttgagt?ttgacaatat?atccctatgt?ttctcttctc 180

cacacccttt?taccgtaatg?cgacagcatt?gtgtagagtg?cattgcctta?attagaacct 240

acgaatcctt?gatttgtata?tgacacccga?tcaccatcga?gccacctaca?tcatggcgcg 300

ctctcagatc?ctccgaacca?aaacagtaat?gtcgatcgtc?gcgcctgaac?caaggctgat 360

tttccctgct?gaagcgtgtc?ataaagtgaa?acatactttg?tagataaact?cggcaattaa 420

ctgccactta?ccccatacat?cgctaccgga?gagtggagcg?agtgcacggc?catcaagtga 480

aactgataac?cggcgatccg?ccggcttatc?ggcgatggcg?ttatcagagg?gaccttagta 540

ggttgcacag?ttcactagta?tttattacta?agtacttcct?tcgccttgct?tattgcccat 600

gattcgtcct?tctctttttc?tttggttaat?tgaactactc?tccatgagag?ttctgtacag 660

gttgagatag?accaaccacc?accaccatgc?cttttccctt?ttcgtccgct?cttctcggct 720

atatcttaac?tacgagcact?actctcacct?ccctagtcgc?aggacagtat?taccctccga 780

cgcctgagga?tctcaccgtt?attcattcgg?agatattccc?tggtgcgagg?atctcctata 840

agcaagtgag?aaatacacca?cccttctcct?caatcccaat?ctaacacccc?atactaatac 900

tatgtgacca?gcccctcggc?atctgcacca?ccaccccctc?cacccccagc?tactccggct 960

acatccacct?ccccccacac?acccttacca?atctctccat?tccaggaatc?agcatctcgc 1020

aaccataccc?tatcaatacc?tttttctggt?actttccttc?ccgccatcac?cacaacaatg 1080

atacatcccc?actcaccatc?tggatgaacg?gcgggcccgg?cggatcctcc?atgattgggc 1140

tatttcaaga?gaacgggcca?tgtactgtga?atacggactc?gaattccacg?gcctataatc 1200

cctggtcgtg?gaatgagtac?gtcgatatgt?tgtatattga?gcagccggtg?cagacgggat 1260

ttagttatga?tgtgttgagg?aatgggacgt?tagatttggt?tagtggggag?atagatgtta 1320

gtattagtga?tggtgagagg?gatggagtag?gacagaatga?gacgtttttg?gtggggacgt 1380

tgccgagtca?ggatgtgcat?gggacggtga?atgggacggt?taatggggga?agggcgcttt 1440

gggttgcgtt?gcaggtttgg?ttgggtgaat?tctctgaata?tgtttcttct?gttgacggga 1500

atggtggtgg?tgatgacagg?gtgagtatat?ggacggagtc?atatggggga?cggtatggac 1560

cggcatacac?ggcgctcttt?caggagatga?atgagaggat?tgagagtggg?gaggtaagca 1620

ccgggaagaa?gatccatttg?gatacgctgg?gcattatcaa?tgggtgtgtg?gatttactcg 1680

tgcaggtccc?ttcgttccct?gagcaggcgt?ataacaatac?gtatgggatc?gagggaatca 1740

atcgcacgct?ctacgaccgg?gctatggata?gttggagcaa?gcctggcggg?tgcagggata 1800

tgatcatcga?gtgtcgcgat?gctggcgagc?tcggagatcc?cctcatgtat?ggcgacaatg 1860

agacggtaaa?tagcatctgc?gaggaggcgt?cggactactg?ttcgcgggag?atcaagagcc 1920

tgtatacgaa?tacctccggg?cgaggatact?acgacatagc?gcatttcacg?ccggatgcag 1980

ctctcgtgcc?ttacttcgtc?gggttcttga?atcgcccatg?ggtgcaaaag?gcacttgggg 2040

tcccggtgaa?ctataccatg?tcgtcagagg?cagtggggaa?cagtttcgcc?tcgacgggcg 2100

attatccgcg?aaatgatccc?cgcggaatga?tcggggatat?tggatacttg?cttgactccg 2160

gtgtcaaggt?ggctatggta?tatggggacc?gggactatgc?ttgtccgtgg?cgcggcgggg 2220

aagatgtcag?cctgctggtg?gagtacgagg?atgcggagaa?gttccgtgct?gctgggtatg 2280

ccgaagtgca?gacgaagtca?tcctacgttg?ggggtctagt?aaggcagtat?gggaacttct 2340

cgttcacgcg?tgtctttcag?gcgggccatg?aggtgccatt?ttatcagccc?gaaacggcgt 2400

atgagatttt?taatcgcgct?cagtttaatt?gggatattgc?gacgggaggc?atttctctgg 2460

agcagaatca?gagctatggg?acggagggac?cgtcgtcaac?gtggcatatc?aaaaacgaag 2520

tgccggagag?ccctgagccg?acgtgctatt?tgttggcgat?ggattcgact?tgtacggatg 2580

agcagaggga?acgggtgctg?agtggggatg?cggtggtgag?ggattgggtt?gttgttgatg 2640

atattgaggc?tgaaagctcg?ttcagcggtg?ttggtgatca?gctggcacag?gtccctttgg 2700

gacattgacg?ttggttgcca?tatgttgaca?gtgttggtgg?atgaaagtga?tatagatgga 2760

taaagttagt?gcataagtgc?atagctgact?aagaatgagc?atcattcata?tatggataag 2820

tctggctagc?tcgatccggc?aagtggccgg?ctgactgcct?aaagtggaag?actggaagcc 2880

ttcagatttc?catcagtcca?tctcaatatc?tccatctggc?ttgtcaaact?cctatatcca 2940

ccggccggac?ccttctatta?ttaacagcgc?cgctatcacc?cggacaatcc?gtatggcatc 3000

acgggttgac?ccctccattg?cggccatcga?accttctcga?accttctatt?ggtcgaccgc 3060

tgcggtcaac?gtcacacgtc?accgcaggct?cacctgaaag?ccgccggcgt?tgcactatac 3120

atcagccctc?gtcctcacac?ctggctttct?ccgctaatcc?tccaagtaca?cccttcactg 3180

tgccttgatg?tcttgaatca?acatctataa?aaactgcatt?a 3221

<210>54

<211>2590

<212>DNA

＜213〉aspergillus niger

<400>54

gagtgagtaa?attgatctaa?cggcgtcgcg?ctgacaaaca?tcatagctat?caatcagaaa 60

aagcgaagca?tatttcacat?acgatcggat?agcctaccaa?ggaactgagg?atatacgtac 120

agctttcgct?tggctgacac?gtaaagtcat?ttggtgattt?tggagcgact?ttggatctcc 180

ttccatgtaa?gttataacct?accgcaattc?caaagtatag?ctagtagctg?tcagtcactc 240

aagaacttgc?agcaaggcga?tggcaagaca?aaacaatcga?tggtacggag?tagatagaag 300

acactaaagc?actttcacat?gacactgttg?acatagtgct?tatgctcttt?acactcaaca 360

tgagtcactt?tgataaggtg?atttcgccag?atggtggcat?tattgactcc?tgattggatc 420

caccgataag?gaaccacttt?ttggctggca?atgtactatg?ttattgacag?gcatcgaact 480

tccccaaatc?tcaggctggg?ccgccagcaa?atggcgcggt?tccggactcc?ggcgggggct 540

gacagggcca?accgttggct?gcttgtccct?tgccgccgga?gagcttattt?aactcttgtc 600

tctcctcttc?ccccacaaag?ctcactcagc?actcaaggcc?tcaccgcgcc?atagtcccgc 660

tcttgatcat?ccttagcaaa?acggctgtaa?atcatgagaa?catctactct?tttgctcctc 720

tggagcactg?caggagcagc?tttggcttct?ccgtacccgc?ttcccgactc?gcaagtagtc 780

ttcgccgcgg?atcacgaggt?cccgaataca?cagggcaaac?acgtcgtgga?cgaggccata 840

ctctcggcgc?tgaacgctca?ttctgaccca?gtcgctgcaa?tggtgtctct?acgtcccgaa 900

actgcagctt?ttctagctga?acctcgtctc?ttgcacattc?ggggcgaaga?gaaggcggaa 960

tggatgaccg?aaggcgacaa?gctgcgcctc?cgccaacgcg?gaaagaagtt?catggacatt 1020

accgagcatc?aggacttcta?cgcagagcag?gcgatggctt?cgtttgctgg?ggatcctagt 1080

taatctccct?tttgtcgagg?taattaatac?tgtgttaacg?ccctttagat?cttcccaagc 1140

tgtcccataa?aggtctcgtc?aagccgctgt?tctctcaaat?cgagacggaa?cgaatgcacg 1200

atatcctgca?gcacatgacc?tcctactaca?atcgatacta?cggtgattat?cacggcgaga 1260

tgagctccga?atggctgcac?gactacattg?ctgcggtatc?tccaccccct?acagccacaa 1320

tttttgaaca?tgaacttact?caaccaagat?catctccaaa?tcgcctttcc?gcacccacat 1380

ctctctcgaa?tacttcaccc?atcctttccg?ccaatcttca?attattgcac?gcttcgagcc 1440

taaagttcgc?agcttctccc?aacctttgac?catcattggt?gcgcaccaag?attcggccaa 1500

ttatcttttt?cccctgctgc?ccgcccctgg?cgctgacgat?gactgttccg?gcactgtcag 1560

tatcctcgag?gccttccgcg?ttctggcgga?gaatggctac?acgcccaagg?acgggcctgt 1620

tgaattccat?tggtatgcgg?ctgaagaggc?cgggctactg?ggcagccaag?ccatcgcgcg 1680

gtacaagaag?gagcagggcg?ctaaaattga?tgccatgatg?gagtttgtag?gttcccatca 1740

tccaacggac?ttggaatcac?ttttgacttt?cggttattaa?catctgcata?ggatatgacg 1800

gcttttattg?cccgtaacgc?caccgagacc?atcgggtttg?ttgcaaccca?agccgatgca 1860

gcgctcacaa?actgggccct?caacctcagt?cgagaataca?tctccattcc?ggcggaagtc 1920

tatgaacttg?gcccgtaaga?ccccagaata?tccccagagg?catgtcaagt?gctgaatgat 1980

ttcttagcaa?cgctggatcc?gactacatgt?catacactaa?gctcaactac?cccgctgcct 2040

ttgcatccga?aggcaacccg?ctcgctgggg?gctctttccc?gggtgaaatg?gacccctacg 2100

tacacggcat?caaggatagg?atggacgttg?acgatgaaac?gggcgtcttc?tctatcgaag 2160

taagttgacc?gcactcaacc?tgtccatctt?cttgctaacg?atttgccagc?acatggctcg 2220

gttctccgag?ttggctatcg?catttgttgt?cgagcaggct?gggtgggata?atacatggcg 2280

gtagagtata?tacagggatg?tttccagctt?catagcatat?agagctggta?tatagtcatt 2340

gtgcttacaa?tatataccat?tctattgagt?tcttgacaga?gttattatgt?cagaatggta 2400

atcagtctaa?atcaatgcgc?ccttaaatca?agaagtacac?tagtgcccat?gaccaaaaca 2460

atttccactt?acacatacca?cctatctgcc?tcactacgaa?acccttacca?tacaatgttt 2520

acgttgtgct?cctttcaata?ctccatttcc?atagttcatg?ctatcgtggg?ataaacatgc 2580

acatatcctc 2590

<210>55

<211>3290

<212>DNA

＜213〉aspergillus niger

<400>55

gagaggcaga?aggagtcatt?tatcacttgt?attccaatgt?attttccatt?tatagatact 60

gcattcaaat?gcaccgttta?gcatagcatc?ccacattcta?tttcattcca?atctcatgcc 120

attgccatcc?ccggtattaa?tttacttctc?cgccttatct?tgcaatcttg?caatctcttt 180

ctcctcgtta?tcacgcgttc?ctgcaggcgc?acctccgatg?gcactgcagc?cggagtcccc 240

gcggcgccgg?cactactaaa?gactaaagtg?tctagtctag?cctccaatgt?gctcacctcc 300

atcagcatct?catccattta?tcttctgacg?atgtcatctg?caggctccac?cccctccggc 360

cgccccgacg?ctctccgacg?gtgcacaaca?atcaattctg?cagtcacgct?caagattcgt 420

ccctgccgga?ctcctcatgc?cgtgcctggt?ttaatctatg?caatggagta?aggtagtatc 480

gcctagcagg?agcggagttc?ctgctgcgct?cacgccatgg?tgccggcgca?gacataaatc 540

gctcgtttcc?tccggcgctg?gccgttctct?cgagccagtt?tgtctgttgt?ggttgtagga 600

tcctctgttc?ccctcgacag?ctcacaatgc?gttccttctc?cgttgtcgct?gccgcgtcac 660

tggcgctctc?ttgggcgtct?ctggcccagg?ctgctcgccc?ccgtcttgtg?cccaagccta 720

tctctcggcc?agcttcgagt?aagtcggctg?cgactacggg?tgaggcttat?tttgagcagc 780

tgctggacca?tcacaacccg?gagaagggaa?cgttttccca?gcggtactgg?tggagtactg 840

aatactgggg?tggacctggg?tcaccggtgc?gtctctgaca?tttggtctta?tgaccggcca 900

tattgaaact?tagccggtgg?caaggtccgc?aatcatgagg?aacattgctg?attaaactag 960

gtggtcctct?ttaaccctgg?agaggtctct?gccgatggct?atgaggggta?tctcaccaac 1020

gatactctca?ctggtgtcta?tgcgcaggag?atccagggtg?ccgtcattct?cattgaacgt 1080

gagtgtcact?gctaccatgg?aaaaaagaca?ttcgctgatc?gaccccaatc?tagaccgcta 1140

ctggggcgac?tcttcgcctt?atgaggtgct?caatgccgaa?acacttcagt?atctcacact 1200

ggatcagtcc?attctggaca?tgacctactt?cgccgagacg?gtaaagctgc?agttcgataa 1260

tagcagccgc?agcaatgcgc?agaatgctgt?atgttacctt?caccgctcta?tgtttctgat 1320

aggtactgac?aacgtagccc?tgggtcatgg?tcggtggctc?atacagcggt?gccttgacgg 1380

cttggaccga?gtctatcgcg?cctggaacgt?tctgggctta?ccatgccacc?agtgcgcctg 1440

tggaggctat?ctatgacttt?gtaggtgtag?cctgctcttg?ttatctatac?ttgcagctaa 1500

ccaagccagt?ggcaatactt?ctaccccatt?cagcaaggta?tggcacagaa?ctgcagcaag 1560

gatgtgtctc?tggtagccga?gtatgtcgac?aaaattggga?agaatggaac?tgccaaggaa 1620

cagcaggagc?tcaaagaatt?gtttggtctg?ggagctgttg?agcattacga?tgactttgcc 1680

gcgtgagtac?ttcaaagtct?atagacgagc?ttttctgaca?ggaacagtgt?cctgcccaac 1740

ggaccgtacc?tctggcaaga?caacgacttt?gtcacaggat?actcttcctt?cttccagttc 1800

tgtgatgctg?tcgaggtgag?ttaccaccag?attcctcttg?attgaagcaa?tatactaacg 1860

gacacagggt?gtcgaagccg?gcgcggcagt?gacccccggc?cccgagggcg?tcggacttga 1920

aaaggccctg?gccaactacg?caaactggtt?caattcaacc?atactcccta?actgtatttc 1980

accatctctt?gtctcgttcc?tctcccttat?cctcccagac?taacctagtg?acagactgcg 2040

caagctacgg?ctactggacc?gacgaatgga?gcgtcgcctg?tttcgacagc?tataatgcct 2100

cgagccccat?cttcaccgac?acctccgtgg?gtaaccctgt?cgaccgccaa?tgggaatggt 2160

tcctctgcaa?cgagcctttc?ttctggtggc?aggagtgcgt?accccttacc?tcattcatga 2220

taacacacga?acaattccac?taacaaagat?ccagcggtgc?ccccgaggga?acctccacta 2280

ttgtgccccg?gctcgtcagc?gcctcctact?ggcaacgcca?atgcccgctc?tacttccccg 2340

aagttaacgg?ctacacgtac?ggcagcgcga?agggtaaaaa?ctccgctacg?gtgaacagct 2400

ggacgggtgg?atgggatatg?acccgcaaca?cgacgcggtt?gatctggacg?aacgggtagg 2460

tctcccccta?atttccgttg?aatgtgatgt?gaagataaac?tcaatgctaa?taaattgaga 2520

aggcaatatg?acccctggcg?cgactccggt?gtgtcgagca?ctttccggcc?cggtggtccg 2580

ctggttagca?cggcgaacga?acccgtgcag?attattccgg?gcgggttcca?ttgctcggac 2640

ttgtatatgg?aggattacta?tgcgaatgag?ggtgtgagga?aggtggttga?taatgaggtg 2700

aagcagatta?aggagtgggt?ggaggagtat?tatgcttgat?gaagatactg?gtggacatat 2760

ggagtgtaca?taagatgaat?ggtcataaaa?tgatgatggt?agatacggct?atggctgttg 2820

attagatggt?cctttcgcat?ttcctaatta?ctgagcacgt?gctccatggt?atgggaagtg 2880

gagacgttgc?tatatatatt?gactgtcggg?ctattgttca?cggcgtagaa?gctagacgct 2940

ttgtctatgt?ggccttcact?aaagaccgtg?actctgccca?gtcttccccc?cttcgaggac 3000

ctggtattag?ccaaacccac?ccacaaacct?aacaaagatc?atcgtgacat?tgaagtcact 3060

ctaggtactg?ctggcgctga?ttacagtggc?tcaattcgaa?catttcaaca?gcacataagg 3120

gaagggtcgc?ttcacttgct?accttgatac?gaaagcagcc?acgcccaaca?cttatagggg 3180

tgacaaccat?cggcatgctg?ggttatctac?tatatctcct?gattctgtgg?atcctggaga 3240

tcgatctggt?acactaatct?actacaatgc?atgtgaagta?gggataggca 3290

<210>56

<211>2044

<212>DNA

＜213〉aspergillus niger

<400>56

ggctttgttg?ggctgagcgc?tacttctttc?tctctcttgg?tctgttcgtt?gctccgccag 60

ttggttcact?cagcctcgta?acatcagtat?accaggctaa?gtcaggactt?tggcccccat 120

actgcttccc?ctttttttat?aaaactcaat?ccttctggaa?aggattctat?ttctcaattc 180

tcagactact?taatacgttc?tttgttttca?aattgttttg?tttctgaaac?ttgccgggcc 240

ctatcccctc?ttttttatag?tccgcctgtc?gacatcatat?ccagagtgag?ccaccatgca 300

gctcctccag?tccctcattg?ttgccgtttg?cttcagctac?ggcgtcctct?ccttacccca 360

tggcccgtca?aaccagcaca?aagcacgttc?cttcaaggtt?gaacgggtcc?gtcgtggaac 420

cggtgctctg?catgggcccg?ctgctctccg?caaagcatac?cggaagtacg?gaatagctcc 480

cagcagtttc?aacatcgatc?tggcagactt?taaacccatt?acgacaaccc?atgctgctgc 540

tgggagcgag?attgcagagc?ctgatcagac?tggcgctgtc?agtgctactt?ccgtcgagaa 600

cgatgccgag?ttcgtttcgc?ctgttcttat?tggcggccag?aagatcgtca?tgacatttga 660

cactggttct?tctgacttgt?aagtcttgga?tgcagctgtt?tactctttgg?tacagtgatt 720

aacgtcgatc?tacagttggg?tgttcgatac?gaatctcaat?gaaaccttga?cgggacacac 780

ggagtacaac?ccttcgaact?cctcgacctt?caagaagatg?gacggataca?ccttcgatgt 840

ctcgtatggt?gacgactcgt?acgcctctgg?ccccgtcgga?acggataccg?tcaacattgg 900

cggcgccatt?gtcaaggagc?aagccttcgg?tgtccccgac?caggtatccc?agtcgttcat 960

cgaggacacg?aactccaacg?gcctggtcgg?gttgggcttt?tcctccatca?acaccatcaa 1020

accggaggcg?caagacacgt?tcttcgccaa?tgtcgcacca?agtctggacg?agcccgtcat 1080

gaccgcctcg?ctcaaggctg?acggagtggg?cgagtacgag?ttcggcacga?tcgacaaaga 1140

caagtaccag?ggcaacattg?ccaacatcag?cgtggactca?tcgaacggat?actggcagtt 1200

ctccactccc?aagtactccg?tggcagacgg?agagctgaag?gacattggaa?gcttgaacac 1260

ctcgatcgcg?gacaccggta?cctcccttat?gctgctggat?gaagacgtgg?ttactgccta 1320

ctatgcgcaa?gttcccaact?cggtctacgt?gagcagtgcc?ggtggttaca?tctacccctg 1380

caacaccact?cttcccagct?tctcgcttgt?cctcggcgag?tcgagcctgg?ccacgatccc 1440

cggtaacctg?atcaatttct?ccaaggttgg?caccaacacc?accaccggac?aggcctgtaa 1500

gttgctcccc?ttcttttgca?tgattgaaca?tgattgactg?attgtgctgg?ttagtgtgct 1560

ttggcggcat?tcaatccaac?ggaaacacct?cgctgcagat?tctgggcgat?attttcctga 1620

aggccttttt?cgttgtcttc?gacatgcgcg?gcccctcgct?tggtgttgcc?tctcccaaga 1680

actagtttcc?ttttcctgta?cttttccccc?gcgtgtaata?atatcgtctg?attttttgga 1740

ctgtctccta?cgtgggcaag?atggatggat?agtttgctca?cgtgcattgc?tttaccttgg 1800

gtctgtgagt?caaggcagga?gtgcgtggct?gtatctacaa?ttcaagttac?agtgccgacc 1860

gttattgcct?tccacatcga?aaaacataga?cactctttct?aaccctaatc?catgatacaa 1920

gtatatactt?cgagtccata?ttatggtggt?gtatcaaggc?gccatgttta?tatctaatga 1980

aaccaacgta?ggtctcatct?tcatacgttg?tttaaaaggt?gccgaagaat?atacgaagat 2040

agat 2044

<210>57

<211>3916

<212>DNA

＜213〉aspergillus niger

<400>57

ttcgcagata?ttcgagtcaa?taccttgtat?attaacggaa?aagggtttgg?gaagcgttgc 60

cttcaaggat?gaaattcaaa?acgtagagta?tcttgacctc?aatgacctca?tgatcccctt 120

gaccgtacag?ctagtgatct?cacggtggta?attgctcgat?tcgattccaa?actacagcaa 180

gaagcatgcc?cgacaggaaa?gtcaagttac?aagagatcta?gaacggggct?gctacggctc 240

tgaccaacct?ttgtctgttc?tagatgaggc?tgcagtgacg?taactccggc?ccaagaaaat 300

gggtggtaac?cgaccgttgg?aaatcccatg?gaactggcgg?ggttcaaatt?cccgataact 360

gtaccgaatc?cttgtatccg?gatataggcg?accgatagta?gtagctatag?tcagacgtag 420

cacagcatta?ctgtgtcccc?ctggtcataa?gagtatttgt?tacaataaga?agggaccgtc 480

tagttgtgta?tcacaacaac?tgacacttgc?ggaacaggta?tcagatcctt?gcgctggcat 540

cgactccagc?aatggcttgc?tctgtttcgg?acgaatatca?tcgggctaga?ctgtgacctg 600

aaggatcgat?ccaggagttt?gacggcttgg?gtttctaatc?cgatctggaa?gattattgtt 660

tagtcaagac?cgggaacagc?aagaaccgga?agattatctg?tttaactact ttgccaaaac 720

agttattcaa?tgtcatgctg?agtgcgatgg?ggacccaagc?tcacgagtga?tgctgatcaa 780

agggtccgtt?cggtttggga?aaggaagggc?gccctgtgct?tgccgttgga?atccacggaa 840

cgattccgat?tgactgcacg?ggatttgcaa?actgactccc?agtgggacga?agaaagaaac 900

ccatcttact?ttgcggaggc?aggatggcac?tttgtgtcgg?aagggtggtg?actcctggtg 960

ttgccgagaa?gatggaatgg?tggagaattg?ccttgaaaat?gaggctgtag?gggcactaac 1020

ccagtcgggc?cgagcgtgac?gcctttcccg?atgaggcagg?tcaggtgggt?gtcacgggtc 1080

tatcttatcg?cttatcatct?gaccggttcc?tggcagaagc?tccccttccc?agctcctcgt 1140

atttattcct?ccccaggttg?ttgtttgctc?ttctccctgt?ctcgccttct?ctccagcctc 1200

cattcaacgg?gctccatccc?cttctctccg?gatccctcta?tcccttattc?ttccttagat 1260

atcattattc?atatagtgcc?ttgtttcgct?caccatgcgc?attgactccg?cggcgctaca 1320

tctggtccca?gtcctcctgg?gccaggtcgg?tgctttacaa?ttacccttgg?tccaagactc 1380

caattcacag?tggcagaaac?caaatgcagg?tgataaaccc?ctaattagct?ctccgttgct 1440

tcaagagcag?gtcaaggcgg?agaatctgtt?ggacagggcc?cggcagcttt?acaagattgc 1500

ggagctggga?gaagacgagt?ataaccaccc?cactcgcgtc?attggcagta?aaggtacgat 1560

atattttttc?atcatgtccc?tggaatacga?taattagctg?acaacctttg?ctccccaggt 1620

caccttggca?cgctcgacta?catatactcc?acccttaccg?acctcggtga?ttattatact 1680

gtcgtcaatc?agtccttccc?tgccgtgagc?ggtaatgtct?tcgagtctcg?ccttgtcctt 1740

ggtcacgatg?ttcccaagtc?agctacacca?atgggtctca?ctcccccaac?gaggaataag 1800

gagccggtat?atggctccct?ggttgctgta?tccaacctcg?ggtgtgaggc?ctcggactac 1860

tcgtccaact?tgaaaggcgc?cgttgcattt?atcagtcggg?gaagctgtcc?gttcgggacc 1920

aagtctcaat?tagctggtaa?agcgggagct?gttgctgccg?tcatctacaa?caacgagcgg 1980

ggtgacctaa?gcggaactct?aggaaaccca?acccccgatc?atgttgctac?ctttggtatc 2040

tcagacgagg?atgctgcccc?agtcctggag?aagttgaata?aaggcgagaa?ggtggacgct 2100

atcgcctacg?ttgatgcgat?agtagagacc?atccacacca?ccaatatcat?cgcgcagacc 2160

acggatggtg?acccgaacaa?ttgtgtaatg?ctgggtggcc?acagtgacag?cgtggccgag 2220

ggcccgggta?tcaatgacga?cgggtccggt?actctgaccc?ttttggagct?tgccacattg 2280

ctcacccagt?tccgtgtcaa?caactgcgtg?cgatttgctt?ggtgggccgc?cgaggaggaa 2340

ggccttctcg?gatctgacta?ttacgtgtcc?gttctcacac?cggaagagaa?ccgcaagatc 2400

cgcttgttca?tggactacga?catgctcggc?tcgccgaact?ttgcgtacca?agtttacaat 2460

gccactaatg?ctgtgaaccc?cgagggatct?gaggagcttc?gtgatctgta?caccgacttt 2520

tacgaagatc?atgggttcaa?ctacacgtac?attccgtttg?acggacgcag?cgactatgat 2580

gccttcattc?ggcatggtat?cccgggtggt?ggcattgcca?cgggagcaga?gggtatcaag 2640

actgtcgagg?aagcggacat?gtttggtggg?gttgctggcc?aatggtatga?cccgtgttac 2700

catcagatct?gcgatacggt?ggccaatgtg?aacttgactg?cgtgggagtg?gaacaccaag 2760

gtaggaccgc?atccagcatt?agttactgtt?atgccgatca?ttcttttgct?aactgggatg 2820

atttacctta?gctcgttgcc?cactccattg?cgacttacgc?caagtccttt?gacggattcc 2880

cggaacggtc?cgatgaaccc?atcagccctg?ctgcttttga?ggaaccgaag?taccatggcc 2940

acgcgttgca?attgtaagat?ccgtgtgccc?caaagcttgg?tagtggggca?aatatctgta 3000

gcagagacca?agtcctttct?cgaacctgag?tcccacttgg?tctttccatc?ttttgagttg 3060

aacaagtaga?ccgttactcg?tcatgtagac?gaagacctgg?cagaaccgtt?gtactatgta 3120

gacatttatc?ggggattgtc?tgttatgaat?tcatgtgctt?gtgggatatg?gttaggtatt 3180

tctcgcaaat?gaccacggct?tcctctatca?tttcccgcct?cgcaccctca?taccgatttc 3240

cgttaactta?atatcatctg?tagacttcgc?ggtaatacta?cagggaccca?gagcgtcctg 3300

tggggagccc?aaatccagaa?tggaacagct?gcatcggtgc?ttaatctatt?gtccatacga 3360

cgcagaggca?ctttcagtct?aagctaaaag?caagcattat?tcagagatac?agtgagtgtc 3420

aatatgccgt?tggttcatgc?gcgattcgtg?caaaaacagc?ccaccgccct?atataacaga 3480

gtatattgct?ctctgcctct?ccgggtagag?atcgacattt?ccgggaccgc?catgaaacgt 3540

tggtgggcct?cggagcgtcg?aggattgaca?ctagggccgc?gctagaatcc?cccagggtct 3600

gcaaaatacc?ctatggggtg?acctcgaaca?gggcgtgaaa?cggcaattcc?tccgaaagcg 3660

ttgcgaaagt?gtctacaaag?tggtgatcca?gaccatcacg?cggttccgga?acaattgccc 3720

ttcttatttg?gcactttacg?gcgtggagtg?gagtcatccc?cctttgttga?aacaacacgg 3780

gcatgtttcc?tttgccgtcc?ggttcgttac?ctataaagac?agggtaggtc?tgttcgggaa 3840

aaagacacaa?tcagcgccta?tcccgtccgt?cgaagtctat?tgccataccc?tatcacggat 3900

catcagac?tc?ataaca 3916

<210>58

<211>1443

<212>DNA

＜213〉aspergillus niger

<400>58

atgcatctcc?cacagcgtct?cgttacagca?gcgtgtcttt?gcgccagtgc?cacggctttc 60

atcccataca?ccatcaaact?cgatacgtcg?gacgacatct?cagcccgtga?ttcattagct 120

cgtcgtttcc?tgccagtacc?aaaaccaagc?gatgctctag?cagacgattc?cacctcatct 180

gccagcgatg?agtccctgtc?actgaacatc?aaaaggattc?ccgttcgtcg?tgacaatgat 240

ttcaagattg?tggtagcgga?aactccctct?tggtctaaca?ccgccgctct?cgatcaagat 300

ggtagcgaca?tttcatacat?ctctgtcgtc?aacattgggt?ctgatgagaa?atctatgtac 360

atgttgctcg?acacaggcgg?ctctgatacc?tgggttttcg?gttccaactg?cacgtccaca 420

ccctgcacga?tgcacaatac?cttcggttcg?gacgattctt?cgacccttga?aatgacatcg 480

gaagagtgga?gtgtgggcta?tggaactggg?tctgtcagcg?gcttgctagg?aaaagacaag 540

ctcacgattg?caaatgtcac?tgtacgcatg?actttcggac?ttgcttccaa?cgcatcggat 600

aacttcgagt?cgtacccaat?ggacggcatt?ctcggtctcg?gtcgaaccaa?cgatagttcc 660

tacgacaacc?caacattcat?ggatgccgtt?gcagaaagta?acgttttcaa?gtcgaatatc 720

gttggcttcg?ccctttcacg?tagccccgcc?aaggatggca?cggtcagctt?tggcactact 780

gacaaggaca?agtacaccgg?cgatatcacc?tacaccgata?ccgtcggatc?ggacagctat 840

tggcgcattc?ccgtggacga?tgtctatgtt?ggcggcactt?catgcgattt?ctccaacaaa 900

tcagccatca?tcgataccgg?aacttcttat?gctatgctgc?cttcaagcga?ctcgaagacg 960

ctgcacagtc?tcattcccgg?cgccaaatct?tcggggagct?accacattat?tccgtgcaac 1020

acaactacta?agctacaagt?ggcattctct?ggtgtgaatt?acaccatctc?gccgaaggac 1080

tacgtgggag?caacttcagg?ttctggatgc?gtttcgaaca?ttatcagcta?cgacttattt 1140

ggtgatgaca?tctggctcct?gggtgacacg?tttctcaaaa?atgtgtatgc?tgtgtttgac 1200

tacgatgagt?tacgggtcgg?atttgcagag?cgttcctcga?acaccacctc?tgcgtcgaac 1260

tctacgagct?ctggaacaag?cagcacctcg?ggatccacta?caacgggcag?ctcaacgact 1320

acgacgagct?ctgctagctc?tagtagttca?tctgatgctg?aatcaggaag?tagcatgacc 1380

attcccgctc?ctcagtattt?cttctctgct?ctggcgattg?cttccttcat?gctttggctc 1440

tag 1443

<210>59

<211>3300

<212>DNA

＜213〉aspergillus niger

<400>59

atgcttcgtg?gtcttcgtga?tgtcgtatta?ttacaatttg?caatcccctt?gttcttgcta 60

ttgcatttta?gattatcgct?acggggtgtg?atcacaggat?ttggttctaa?atcacatttc 120

cagagaccat?tgagcaaaat?gtcatctact?caaaagagcc?atttcaagct?actccagaag 180

ttcaaaccgg?agtactcgcc?tagcgagttt?gctcagtatg?agtcggagag?aacaggcatg 240

agggtagtgg?tcattgacca?aaaaggaccc?aaagtcacag?gttattttgt?tctagccaca 300

gagattctcg?atgattcagg?tgctcctcac?acgttggagc?acttgtgctt?tatgggctcg 360

cggaactata?gatataaggg?cttccttgac?aagctagcaa?cacgtgttta?ttcgagcacc 420

aatgcctgga?cggccacaga?ccacacggcc?tacaccttgg?acacagcagg?ctgggaaggg 480

ttcgctcaaa?tcttgcccgt?gtacctagag?catgttatag?ctccaacact?gacagatgaa 540

gggtgctata?ccgaagtgca?tcatattgat?ggcgctggag?acgacgctgg?agtcgtctac 600

tcggagatgc?agggtgtgca?gaataactct?gcagagttaa?tcgatctaac?cgctcgtcga 660

ttgacttacc?cgcatggtgt?aggttttcgc?tacgagacag?gcggtatgat?ggagcagctc 720

cgcgtcctca?ccgcggaccg?tatccgagcg?ttccatcgtg?agatgtacca?gcccaagaac 780

ttatgcctaa?tcatcacagg?cgaagtagat?caccagaaca?tgctggagac?cttggacaag 840

ttcgaagata?ctattctaga?tgtcattccc?agtcctgatt?cacctttcaa?gaggccgtgg 900

gtagattcca?agcaggcgcc?gccattggag?aagtccattg?tccagactgt?ggaatttccg 960

gaagaagatg?aatctttcgg?ggagatagaa?attagattcc?tcggtccgga?ctgtaccgac 1020

cctgttcaaa?ccggggctgt?caatgttgca?ttgctgtatc?tggccggttc?atctgcttct 1080

ctattggata?acatcctggt?tgagaaggag?cagctcgcca?gtgctgtcta?ttatgctacc 1140

gaagatcatc?ccagcattga?gatccgcttc?acattaacca?gtgtggagac?agagaaactc 1200

gcgaaggtag?agcaacggtt?tttcgaagtg?ctcaaggacg?ctatggagaa?agatttagac 1260

atgaggtata?tcaaggagtg?cattgaccgg?caaagacgga?cctggaagtt?ctctaccgaa 1320

agctccgcct?cttcctttgc?ggagtacgtg?atctcggatt?ttcttttcgg?aaagagagac 1380

ggatcgacta?tgcttgatgt?tgcgaccttg?caagagtacg?acgtgctgga?gaagtggagt 1440

gaagaacagt?ggcgcagttt?tatcaaaaca?tggatttctg?atgccaacca?tgtcactatc 1500

cttggtgttc?cgtccgttaa?gatgtctgac?acattaaaga?aggaggagga?agctagagtc 1560

gcagagcaaa?agaagcgctt?gggtgatgag?gggctgaaga?agttggccga?caagctggaa 1620

aaagctaaag?ctgaaaatga?caaggagatc?cccaaggaga?tgctggagag?gttccaaatc 1680

cctggaatag?agtctatcca?tttcgtggac?actactacag?ccaggtctgg?tgcagccctc 1740

gatgccgggc?gcccatccca?caaggcgcaa?aaactggtgg?atgctgatgg?ctctgatctg 1800

cccttgttca?tccatttcga?gcatatcccc?agtagcttcg?tgcagctctc?cctcctcatc 1860

tcggcacagg?ccgtacctgt?gcagcttcgt?ccactgctgt?ctgtgtatac?tgaggcattc 1920

ttcaacctgc?ctgtcaaccg?gaacggggaa?accatcaact?ttgagcaggt?ggttgtcgag 1980

ttggaaaggg?atactgttgg?ctactccatg?gaaggagcta?gaagcctagg?aaactcggag 2040

atgttgcgga?tctcattcca?ggtggagctt?gagaagtatc?acacggcgat?cgcatggatc 2100

caggaacttt?cctggaactc?gattttcgat?gtcgagcgac?tccgagcgat?taccagtcga 2160

ctgctctccg?atgtgcccga?ttccaagcgt?agtggcgacg?acatgctcgc?ggctgttcat 2220

gtgatggtcc?actatgcagc?agagtctatt?gttcgggctc?ggagcacctt?ggtgaaggcg 2280

cgttatttga?aacggatcaa?gaagcaatta?gcagaagagc?cgaagtctgt?cgttgcgcgg 2340

atggaagaaa?tcagagatgc?gcttttccgt?ttcgagaaca?tgcgagtctt?agttatcgct 2400

gacctggaga?aacttcaaaa?ccctgtgtca?gcatggaaac?catttgctga?gcgtttgggt 2460

gcaggtgccc?ctctacagcc?tatcacgact?agaagaccgt?tgctcagtga?ggcaggccag 2520

aagttgggcg?gtaagtcgta?tgtggttcct?atgccgacga?ttgattcatc?gttcgcatat 2580

gctaccgcac?gtggtttgga?ttcttatgat?gatccaagac?ttcctgcctt?aatggttgca 2640

attgcataca?tgaacgcggt?tgagggtccc?ctctgggttg?cagttcgagg?caagggtttg 2700

gcatatggca?cgaactttgc?ctataacatt?gataccggat?tcgtcaactt?cgacgtttac 2760

cgctccccca?acgcccataa?agccttcgac?tccagcaagc?agattgttga?ggatcacctc 2820

tctggtgcga?tgcccttcga?tcccttgatg?ctggagggtt?ccattagcag?cattgtggta 2880

agctttgcga?atgaacagtc?gacaattggt?agcgcagcct?caggcagttt?catccgacag 2940

gtgattcggc?gcctgcctag?cgactacaag?gagcgggtgc?tcaagcaggt?gcgggctact 3000

agcgttgatg?acgtgaaagg?cgctctgaag?gacatcattc?tgcctttgtt?taacccgtcc 3060

acggccaata?tcgtggttac?ctgcgctaca?gtgcttgagg?agactatcaa?ggaaggtctc 3120

caggcatcgg?gattcacgcc?tgcggtgcag?ccactcaaag?aattcgaaga?tgactatggg 3180

ctgaaggtcg?gcgatgacga?ggacgaggag?tccgacgatg?acgacgatga?gtatgaaacc 3240

ggatctgaag?atgaagatga?cagtgatgaa?gacatggagg?atgacgaaga?tgatgagtga 3300

<210>60

<211>2181

<212>DNA

＜213〉aspergillus niger

<400>60

atgggagctc?ttcagtggct?gtccatcacg?gctgctgcgg?cctccgcagt?gtcagccttg 60

accccggagc?agatgatcgg?tgccccacgg?agaaccgaag?ttataccaaa?cccctccggt 120

gacaccggtc?tattctcgac?ctcccaatgg?tcgtttgaca?ctcattctga?gagcacctgg 180

tggagcttga?tcgacctcca?atcgggcaag?accaccactc?tcaccgatga?tagcgatatc 240

gaggagatca?tctggcttgg?ctctgacaat?tctacgctcc?tctacatcaacagcaccaac 300

gcgcaggttc?ccggtggcgt?ggagctgtgg?attgcggact?cttctgactt?tgcaaatgct 360

tacaaggcag?cctctctctc?cgccggtttt?ctcggcatca?aatcaaccgt?gacagattcc 420

ggcgacgtgc?atttcatcct?tcgtggaaag?tcctatccca?acggaacggc?atacaatgat 480

cagctcgccg?agacctatcc?cagtacagcc?cgcatctacg?acagcatctt?tgtgcggcac 540

tgggacactt?acctgaccac?cgcctcccac?gctgtattct?ccggtaccct?gcaaagctcg 600

accagcgacg?acggcaatgt?tcaatatacc?tcttcagggg?gattgaccaa?cctggttaac 660

ccagtcaagg?gtgccgaaag?cccattccct?ccttttggag?gcaacgacga?ctatgacctc 720

tcgcctgacg?gcaaatgggt?taccttcaag?agcaaagcgc?cagagctgcc?tcttgctaac 780

aacacggctg?cctatgtcta?tctcgtccca?cacgacggct?ctgcgactgc?ctttgccgtc 840

aacggccctg?atagtcctgc?aaccccggag?ggagttgaag?gagaatccaa?caatcccgtg 900

ttctcccctg?atagcgacaa?aatagcgtac?ttccaaatgg?ctactaatac?atacgagtcg 960

gaccgcaacg?tgctatacgt?atactccatc?gccgatgaca?ccatcactcc?ccttgcaaag 1020

gactgggacc?gatcgcctag?ctccgtgaca?tgggtcgatg?gagacaacct?cgtcgtggca 1080

agccaagatc?taggacgaac?cagacttttc?gccatcccag?gcgatgcagg?ggacgacttc 1140

aagcccacga?acttcaccga?cggcggctcc?gtgtcggctc?aatacgtcct?atccaactct 1200

accctcctcg?tcacgtccag?cgccttctgg?acaagctgga?gcgtctacac?cgccagccct 1260

gacgagggcg?tgatcaacac?actggcctca?gccaacgaga?tcgaccccga?gcttagcggc 1320

cttagttcct?ccgactttga?agagttctac?tttgacggca?actggactac?cctccaagga 1380

tggatcacct?acccccaaga?cttcgactca?tccaagaaat?accccctcgc?cttcctcatc 1440

cacggcggcc?ccgaagacgc?ctgggcggat?gaatggaacc?tgaaatggca?ctccaaggtc 1500

ttcgccgacc?agggatacgt?cgtcgtccag?ccaaacccca?caggaagcac?cgggttcggc 1560

cagcagctca?cagacgctat?ccaacttaac?tggaccggcg?ccgcctacga?cgacctaacc 1620

aaagcctggc?aatacgtgca?cgatacctac?gacttcatcg?acacggacaa?cggcgtcgcc 1680

gcgggtccca?gcttcggcgc?gttcatgatc?acctggatcc?agggcgatga?ctttggacgc 1740

aagttcaagg?cgctggttag?ccatgatggt?ccgttcattg?gcgatgcgtg?ggtcgagacg 1800

gatgagttat?ggtttgttga?gcatgagttc?aacggcacct?tctggcaagc?gcgcgacgca 1860

ttccacaaca?cggatccatc?cggccccagc?cgcgtcctcg?catacagcac?cccccagctc 1920

gtcatccaca?gtgacaagga?ttatcgcata?cctgtggcaa?atgggattgg?actgtttaat 1980

acgctgcagg?agaggggcgt?gcccagtcgg?tttttgaatt?tcccggatga?ggatcattgg 2040

gtcaccgggc?aagaaaacag?cctcgtctgg?tatcagcagg?tgctgggatg?gattaatcgg 2100

tattctgggg?tgggagggtc?gaatcctgat?gcgattgctt?tggaggatac?ggtgaatccg 2160

gtggtggatt?tgaatccttg?a 2181

<210>61

<211>1695

<212>DNA

＜213〉aspergillus niger

<400>61

atgacgaggc?agacttctct?cgttcccagg?ctactaacgc?tagcctcact?agctgcactt 60

tcacaagcag?agctaggcaa?gatccaatgg?aaaggatctt?gcaacttgac?cacttatccg 120

gcattgatct?gtggaacact?agacgtgcca?tacgactaca?cggagtcaaa?ttccagcaag 180

acactgactc?tcgacatcgc?caagtggcca?gcgaccaaga?aaccagtctc?ggagcccatc 240

atatttaact?ttggaggacc?tggtgtcaat?tcgttcgagg?gccttgggct?ttatggagag 300

gaatttcagg?ctattcttgg?aggtcacaat?gatttgatag?cttttaacaa?ccgaggcgtt 360

ggaaacacca?tcccgttctc?ctgctacagc?gatgacgcca?cccgtgaact?cgtcgccctt 420

caagctccta?acgacggcag?agcgtccagc?acggctttgg?gagaaatctg?ggcccagaac 480

gcaaacatcg?cacaggcatg?ctatgctacg?aacaatcaaa?ctggtagtct?tattggaact 540

agctttgctg?caagggacat?catgcaggtc?gctgatgcgc?tcagtggaaa?ggatagtttg 600

gtcaactact?ggggattctc?atacggcact?acaatcggtg?ctgttctcgc?agccatgttc 660

ccggatcgaa?tggggaatgt?cgcgcttgac?ggagtggaca?accccagaga?ggctctttat 720

ggatacaacg?cacaagcggt?tgtggacgtc?gacaaagttt?tcgaaggatt?ctgcacgggc 780

tgcatggccg?caccggacct?ctgccctatc?gccaaggagt?acaccagcgc?cgccaacttg 840

gaagccgcaa?tttacctgat?gctggaaaac?ctcaagtaca?acccgattgc?cattcccgaa 900

accggtggaa?tcgtaacttg?gagcgacgtc?aagtcgacca?tttttgaggc?catgtacctg 960

ccaagctctt?ggcccttgac?ctctgagctt?ctttactacg?tgcaaacccg?caacacaacg 1020

atccttggca?actctgaagt?atacgacacc?atcaaatcct?acggtcaatc?ggcttctttg 1080

acttcggctt?ccgatgaggt?cggcacggcc?attacatgct?ccgacaagca?tcgatctgcc 1140

accattaaag?aggtcctccc?gtacgtcaaa?gccagacagg?ctctgaccaa?gatcggaagt 1200

gatggctcgg?acggcgacat?gagatgcgcg?cagtggaatc?cgaagatgtt?cgccaaggag 1260

cgctactccg?gtgactttga?agtcaagaca?gccaacccgg?tgttgattct?gagcaacact 1320

tacgatccag?cgactcctct?tcccgcagcg?aagaacctga?cagagacctt?tgagggaagt 1380

gtcttgctcg?agcagaacgg?atacggtcat?actaccctgt?ctatgccatc?tctttgcact 1440

gccaaggccg?tccgggctta?cttcaccaat?ggcacattgc?ccgctgacgg?aacgatctgc 1500

caggtggacg?tgcctctgtt?cacgaacttg?acctacaagg?atgtgtggcc?gaagagtttc 1560

caacggagcg?ttgagtcgag?ggatgatgcg?actatcctca?aggctttgat?gtcggtccgt 1620

gataagatgt?cgcgacgcag?gatgtgtatt?tatttgtaca?ccaacagcgc?ttcatggaga 1680

ccggaacttc?cctga 1695

<210>62

<211>1581

<212>DNA

＜213〉aspergillus niger

<400>62

atgtactact?ctctctgggt?tgctgccttg?gtggccgcgc?tgcccgtctc?ccgggcccag 60

tttgtggctc?cgcccacgga?tctcattccc?accaagggat?atctcgacat?ccccgtccgc 120

tacaaacagg?tccccaccgg?catttgtgag?actgatccca?gtgtcaagag?cttctccggt 180

tacgtcgatg?tcgctgagca?tgagcacatc?ttcttctggt?tcttcgaggc?gcgcaaccaa 240

gatcccaccg?aggctccctt?gaccgtctgg?atcaatggag?gcatgtctga?ccccggtcct 300

ggttcctcct?ccatgatcgg?cttgttccaa?gagcacggcc?catgcggcat?tgacgccaat 360

ggctccgtct?acaacaaccc?ctactcctgg?aacaacgcca?gcaacatgct?ctacatcgac 420

cagcccgtgc?agaccggctt?ctcctacagc?attccggttc?ccggctatgt?ggattcttcc 480

acagacaatg?gttttatggg?cgcatttcct?cagtactcgc?gcgaaacctt?ccacttcacc 540

acggagagtt?atggcggcca?ctacgggccc?gtcttcaacg?agtacatcga?ggagcagaac 600

gcccatctcc?agccgggagc?caagaagatc?caactgggca?gtgtgatgat?cggcaatggc 660

tggtatgacc?cgattattca?ataccaggcc?tactacaact?ttacggtata?tccgggcaac 720

acatacgact?acctgccatt?caacaagtcc?atcagctcgc?tgatgtacaa?caacctctat 780

ggccccggaa?actgcctcga?ccagctctac?gactgcgccg?cccgaggcat?cgacgagatc 840

tgcagcactg?ccgacgattt?ttgcgccaac?gaggtcgaaa?acgtctacga?catttactcc 900

ggtcgggatg?agtatgactt?tcgtgaactc?actccggacc?cgttccctta?cgagttctac 960

gttgactacc?tgaacaaagc?gtccgtgcag?gccgccatcg?gcgcatacat?caattacacg 1020

gagagcaaca?acgctgttgg?actcgccttt?tcgtccaccg?gtgacgacgg?gcgactcatg 1080

aacaccatcc?aggatgtggg?caagctgctc?aaacagggtg?tcacggtggt?catgtacgcc 1140

ggggatgccg?actataactg?caactggctg?ggtggggaag?ccgtgtcgtt?gcaggtcaag 1200

gccgccaact?tcagtagtgc?gggttacacc?aacattgtca?cctcggatgg?agtgacacac 1260

ggccaggtgc?gccaggcggg?gcaatttgcc?tttgtgcgag?tgtatgagag?tggacatgag 1320

gttcccttct?atcaaccctt?gcttgcgctg?gagatgtttg?agcgcgtcat?tggcggcaag 1380

gatgtggcga?cgggaaagat?tcccatctcg?tcgagtttac?agacggtggg?cacgcccaag 1440

agttactacc?gggagggcaa?cagcacgatt?cagtgggagg?tgttggattc?tctggcgacg 1500

tacaacacaa?ccacgaatgc?tccgaacccg?gtgagccgga?ggctgaagcg?gatgggacca 1560

gctttgcggt?ttcagatgta?g 1581

<210>63

<211>3471

<212>DNA

＜213〉aspergillus niger

<400>63

atgtcttgcg?tctggctcca?catccacaaa?aggagcctac?tgtctgtcgc?tacgaacaat 60

tctgttgcga?gggccgctgc?ctctacctcc?gccgcgccgc?cgccgccgtc?atcgccgccg 120

cctggttcta?atacttattc?gcctctttat?cgccccatca?ccaatcccat?cggatttact 180

ttgtcgcctg?cgaggtcact?agtttctcgc?aatcctaaat?ttcctgccta?tcggcgctct 240

agtcgacact?tttctttgtg?cccggccgct?gcaacgcccg?gtgtcaccac?gagcatctgc 300

cctggtcagg?cgcccgtccg?ctctctcagc?tcgctcatta?tacactctac?gagaccccgt 360

gctatacgta?tccgtaccga?ccagatggat?ttgaatggag?acgcaggcgc?caagcgcaag 420

cgcagctcca?tcaccacacc?cgccgaacgg?cccgtaaagc?accttcgccc?cgaatcgagc 480

gcattgacac?ccggggattc?gacgcctgcc?aatgggactg?tatacgatgt?ggaggatgat 540

gaagatgcga?gtcgtctgct?gcctgtaggg?cctgctcagg?ccgactcacc?ggaatggcaa 600

gctaccatag?aggaggttgt?gaaaagcgta?gtgtctatcc?acttctgtca?gacctgctcc 660

ttcgacacgg?agctgtccat?gagtagtcag?gctactgggt?ttgtggtaga?tgcagagaat 720

gggtacatat?tgacaaaccg?acacgtggtt?tgcccgggac?ctttctgggg?atactgcatc 780

tttgataacc?atgaggaatg?cgacgttcgt?cctgtgtatc?gggaccctgt?tcacgacttt 840

ggaattttga?aattcgaccc?gaaggctatt?cgatatatga?aattgaggga?actgaaactg 900

cagccggatg?cagctaaagt?gggatcagaa?attcgcgttg?tgggtaatga?tgcaggagaa 960

aaactgagta?ttctgtctgg?tgtcattagt?cggctggata?gaaacgcgcc?cgaatacggc 1020

gatggctaca?gtgacttcaa?tacgaattac?atccaggccg?ccgcagcagc?tagcggtgga 1080

agttccggca?gtcctgtagt?taacattgat?ggccatgcga?ttgctctgca?ggccggtggt 1140

cgtgcagacg?gtgcagcgac?ggattacttc?ctccctctgg?accgaccgct?acgcgcactg 1200

gaatgcatcc?gtcgcggaga?gcctgtcacg?cgtggaacga?ttcagacgca?gtggatcttg 1260

aagccgttcg?acgagtgtcg?tcggttgggc?ttgacgcctg?agtgggaggc?gaccgtgcgt 1320

aaagcagcgc?ccacggaaac?cagcatgctg?gtggccgaga?tcatcctgcc?tgaaggcccg 1380

gcggacggaa?agctcgagga?aggagacgtg?ctcctgcagg?tcaacggggt?gcttctcacc 1440

caattcatcc?ggttggatga?catcctggat?tcgagtgttg?ggcagacagt?gcgtctgctt 1500

gtccaaagag?gcggtcagaa?tgtggagatt?gagtgccagg?ttggcgacct?gcatgccatc 1560

acgcccgacc?ggttcgtgac?ggtggctgga?ggcacgttcc?ataacctgtc?ttaccagcag 1620

tcgcggctgt?atgccatcgc?tactcgcggt?gtctacgtct?gcgaggctgc?cggctccttc 1680

aaactggaaa?acacactgtc?aggatggatc?atcgactcgg?tggacaagcg?gcccactcgc 1740

aatctggatg?agttcgtgga?ggtgatgcga?acgattcccg?atcgttcgcg?cgtggtcatc 1800

tcgtatcggc?atattcgcga?tctccacacc?cgaggcacca?gcatcgtcta?tatcgatcga 1860

cactggcacc?ccaagatgcg?actggctgtg?cgcaacgacg?acaccggtct?gtgggacttt 1920

tcggacctcg?cggaccctat?cccagctctt?cctccggttc?cgaggaaagc?cgatttcatt 1980

caactcgatg?gtgttagcca?gcctgctgcg?gccgacattg?tgcgcagctt?cgtacgagta 2040

tcctgtacga?tgcccctgaa?gctggacggc?tacccccagg?ccaagaagac?tgggttcgga 2100

ttggtcgtcg?atgcagagaa?gggtttggtg?gttgtgtcgc?gagcgatcgt?gccgtacgac 2160

ctctgcgaca?tcaacgtcac?ggtggccgac?tccatcatcg?tgaacgctaa?agtagttttc 2220

ctgcatccgc?tccaaaacta?cagcatcatc?cagtacgacc?caagcctggt?gcaggcgccg 2280

gttcagagtg?ccaaactcgc?caccgactac?atcaagcagg?gacaggacac?gatctttgtg 2340

ggattcaacc?agaacttccg?gattgtcgtg?gccaagaccg?ccgtaaccga?catcaccact 2400

gtttctattc?cagccaacgc?gtccgcaccg?cgctaccgcg?cgatcaacct?ggacgccatc 2460

actgtggaca?ccggactcag?cgggcagtgt?tctaacggtg?tcctgattgg?cgaggacgga 2520

gtggtgcagg?cattgtggtt?gaactatctt?ggagaacgca?catctaattc?gcataaggat 2580

gtggaatacc?atctaggatt?tgcgactcca?tctcttcttc?ctgtcctgtc?gaaggtgcag 2640

cagggagaga?tgccggaatt?gcggattctg?aacatggaga?gctacgtggt?ccagatgagt 2700

caagctcgta?tcatgggcgt?gtcggaggaa?tggatcgaga?aggtgacgca?agctaaccca 2760

tcgcggcatc?agctcttcat?ggtgcgcaag?gtcgattgcc?caccgcctgg?gttcaactca 2820

gcggccgaca?cgttcgagga?gggtgatatc?atcctgacct?tggacggaca?gctgatcacc 2880

cgcgtctcgg?agttggatat?catgtacgag?aaggatacgc?tggaagccct?gattgttcga 2940

aatggacaag?aaatgcggat?ccaggtgccg?actgttccaa?cagaggacct?agagactgac 3000

cgtgcggtcg?tgttctgtgg?tgctgtgttg?cagaaaccac?accatgcggt?ccgtcagcag 3060

atttctaagc?tacacagcga?agtctacgtc?agcgcaagaa?gtcgcggatc?cccctcctac 3120

caatacggct?tggccccaac?caatttcatc?accgccgtaa?acggcgttcc?aaccccgaac 3180

ctggaccgct?tctccgaaga?agtgagcaaa?atccccgaca?acacatattt?ccgcctacgg 3240

gcggtgacat?tcgacaatgt?gccgtgggta?gtgaccgtga?agaagaacga?tcattacttc 3300

cccatgtccg?agtatatcaa?agaccagtcc?cagccttccg?gttggcggac?cgtgtctcac 3360

gacaaggata?aatataaaga?cggcattgca?ccggatgctg?cgaacttgaa?cccggatgct 3420

atggacgaag?ggtttgatgg?agtcagtgat?attgagccgg?atttggagtg?a 3471

<210>64

<211>1611

<212>DNA

＜213〉aspergillus niger

<400>64

atgagagtcc?ttccagctgc?tatgctggtt?ggagcggcca?cggcggccgt?tcctcccttc 60

cagcaggtcc?ttggaggtaa?cggtgccaag?cacggtgccg?accatgcggc?cgaggtccct 120

gcggatcaca?gtgccgacgg?gttctccaag?ccgctgcacg?cattccagga?ggaactgaag 180

tctctctctg?acgaggctcg?taagctttgg?gatgaggtgg?ccagcttctt?cccggagagc 240

atggatcaga?accctctctt?ttccctcccc?aagaagcaca?accgccgtcc?cgactcgcac 300

tgggaccaca?tcgtcgatgg?caagctggaa?gcctatgatc?tcagggtcaa?gaagaccgat 360

cctggctctc?ttggcatcga?ccccggcgtg?aagcagtaca?ccggttatct?cgatgacaac 420

gagaatgata?agcatttgtt?ctactggttc?ttcgagtctc?gcaatgaccc?cgagaatgat 480

cccgttgttc?tgtggctgaa?cggtggccct?gggtgctctt?ccctcaccgg?tctcttcatg 540

gagcttggcc?ctagcagcat?caacaagaag?atccagccgg?tctacaatga?ctacgcttgg 600

aactccaacg?cgtccgtgat?cttccttgac?cagcctgtca?atgtcggtta?ctcctacagt 660

aactctgctg?tcagcgacac?ggtcgctgct?ggcaaggacg?tctatgcctt?gcttaccctc 720

ttcttcaaac?aattccccga?gtatgctaag?caggacttcc?acattgccgg?tgaatcttat 780

gctggtcact?atatccccgt?cttcgcttcg?gagatcctgt?ctcacaagaa?gcgcaacatc 840

aacctgcagt?ccgttctcat?tggcaacggt?ctcaccgacg?gatacaccca?gtacgagtac 900

taccgtccca?tggcctgcgg?tgacggcggt?tacccagctg?tcttggacga?gagctcctgc 960

cagtccatgg?acaacgctct?tcctcgctgc?cagtctatga?ttgagtcttg?ctacagttcc 1020

gagagcgctt?gggtttgtgt?cccggcctcc?atctactgta?acaacgccct?ccttgcccct 1080

taccagcgca?ctgggcagaa?cgtctatgat?gtccgtggta?agtgcgagga?tagctctaac 1140

ctttgctact?cggctatggg?ctacgtcagc?gactacctga?acaagcccga?agtcatcgag 1200

gctgttggcg?ctgaggtcaa?cggctacgac?tcgtgcaact?ttgacatcaa?ccgcaacttc 1260

ctcttccacg?gtgactggat?gaagccctac?caccgcctcg?ttccgggact?cctggagcag 1320

atccctgtct?tgatctatgc?cggtgatgct?gatttcattt?gcaactggct?gggcaacaag 1380

gcctggactg?aagccctgga?gtggcccgga?caggctgaat?atgcctccgc?tgagctggag 1440

gatctggtca?ttgtcgacaa?tgagcacacg?ggcaagaaga?ttggccaggt?taagtcccat 1500

ggcaacttca?ccttcatgcg?tctctatggt?ggtggccaca?tggtcccgat?ggaccagccc 1560

gagtcgagtc?tcgagttctt?caaccgctgg?ttgggaggtg?aatggttcta?a 1611

<210>65

<211>840

<212>DNA

＜213〉aspergillus niger

<400>65

atgaagttca?caaattatct?cttgacgact?gcaacgctcg?caagcagtgt?cctagcggct 60

cctgctcccc?gcaccggttt?ggaggacaga?ctccgtgccc?ggtcattgca?gcgtcaatca 120

catcctctgg?cacctattcc?acttgacaca?tccaccaaag?agaattccag?actcctcgaa 180

gccgacgaga?ataccaccca?tgttacatac?agcagtaact?gggcgggcgc?agtgcgcgag 240

caaccacctc?cgcaaggcac?gtattctgcc?gtgtcggcaa?cctttcgtgt?accagaaccc 300

acggcgcaag?gggggagcgg?aacgcaggct?gggtcggcct?gggtcgggat?agatggcgac 360

acatacagca?acgccattct?acagacagga?gtcgacttct?acgtggaaaa?cgggcagacg 420

tacaacgatg?cctggtatga?gtggtaccca?gactatgcat?atgacttcga?cctagatgta 480

agcacagggg?acacgatcgt?cgccaaggtg?gaagccatct?cgccaagtca?aggtgtagcc 540

actattgaga?acatatcgac?ggggaagaag?gccacgcaga?cgatcagagc?cccagctgcg 600

acagctaccc?ttgccggcca?gaatgccgac?tggatcgtgg?aggatttcca?gtctggcgac 660

tcaatggtcg?atctggctgg?ctttggcgag?atcagcttct?ggggcgtgca?agcacaagga 720

ggagggtcta?catggggtgt?agatgatgcg?actattgtcg?aactgaagca?gggcaacgaa 780

gtgttgacag?acgtggaggt?gcaaagtgat?tcggccttta?cggtgaaata?tacgagctga 840

<210>66

<211>1722

<212>DNA

＜213〉aspergillus niger

<400>66

atgatatatg?tcaactatat?cctgggactt?ctgtccctct?tacacaccgc?tgtagccaca 60

gctcctgatt?atgtcgtggt?agaccaactg?aacagcatcc?ccgacggatg?gacaaaaggc 120

gcagctcccc?cgccatttac?tccgatgaag?ttctggttgt?cgatgcatca?cgagtacaag 180

gcggacttcg?agcagaaagt?catcgatatc?tcgacacccg?gtcaccggga?ttatggacgg 240

catatgaaac?gcaacgatgt?catggccttt?atgcgcccat?ccgatcaggt?ctcaaagatc 300

atcttctctt?ggcttgagtc?ggagcatgtt?ccaccaaatg?ccatcgaaga?tcgcggggat 360

tgggtcgcct?tcacagtccc?gttggcccaa?gcacaatcaa?tgatgaagac?cgatttttac 420

aacttccacc?acctggaaac?aaacacaacc?caaattagga?ccctcaagta?ctccgttccc 480

gagcaagtcg?atgctcatct?gcaaatgatc?cagccaacga?ctcgcttcgg?ccgacctaag 540

acacaaacca?gcctaccgag?cctcatgcca?gtgtcggtta?acattgatga?aataagcgaa 600

gactgcttga?caggcgtgac?gcccatttgc?cttcgccagc?tctatggttt?acctagcacc 660

aaggcaagcc?ccgactcgag?aaacgtcctc?ggaatttccg?gctatctgga?ccagtacgcg 720

cgctacagtg?acctcgacga?gtttctagcc?gtatactctc?caaacagcgt?agacgccgac 780

ttctccgtag?tatcgatcaa?cggaggccaa?aacccacaaa?actcacaaga?gggaagcaca 840

gaggccagtc?tcgacatcca?atacgccctc?tccatggcat?ttgacgctaa?cgcgactttc 900

tacactaccg?ccggacgtgc?gccatccccg?tatctcgaac?agctccagta?tctggtgggt 960

cttccggacg?aggatcttcc?tgcagtgctt?agcacgtctt?acggcgagga?tgagcaaagt 1020

ctgccggagg?aatacacaga?agccacgtgc?aatttatttg?cccaattagg?tgcacgcggg 1080

gtctcggtga?tcttcagcag?cggagactcg?ggcgtcggag?gatcgtgtgt?atctaacgac 1140

ggaagccaga?ggacccgctt?tcagcctatc?ttcccggcgt?cgtgcccgtt?tgttacatcc 1200

gtgggtggga?ctgagggcgt?cgggccggaa?aaggctgtgg?acttttcgag?tggagggttc 1260

tccgagcgct?ttgctcgccc?gtcgtaccag?aatgcgagtg?tggaagcata?ccttgcccgc 1320

ttaggagata?aatgggatgg?attgtataat?ccagacggac?ggggtattcc?tgatgtgtcg 1380

gcccaggcta?gcaactatgt?aatcagggac?catgggcaat?ggctacaaac?tgcgggaaca 1440

agtgctgccg?cccctgtctt?tgcagcagtc?atctctcgac?tgaacgctgc?acgtctcgag 1500

cagggtaaac?ctacactagg?gtttctgaat?ccttggctgt?actcactcga?ccagcaagga 1560

tttacggata?ttgtagacgg?cggatcagtg?ggttgtgacg?ggtcaaatgg?aggagctctt 1620

gtcccgtatg?ccagttggaa?tgccaccaag?ggatgggatc?cggttactgg?gctggggaca 1680

cctctgtatc?agactctgga?gcagttggcg?cagtctgctt?ag 1722

<210>67

<211>1758

<212>DNA

＜213〉aspergillus niger

<400>67

atgcgttctt?ccggtctcta?cacagcactc?ctgtgctccc?tggccgcctc?gaccaacgcg 60

attgtccatg?aaaagctcgc?cgcggtcccc?tccggctggc?atcatgtcga?agatgctggc 120

tccgaccacc?agataagctt?gtcgatcgcg?ctggcacgca?agaacctcga?tcagcttgaa 180

tccaagctga?aagacttgtc?aacacctggc?gaatcgcaat?acggccagtg?gctggaccag 240

gaggatgtcg?acacgctgtt?cccggtggcc?agcgacaagg?ctgtgattaa?ctggctgcgc 300

agcgccaaca?tcacccatat?ttcccgccag?ggcagcttgg?tgaactttgc?gaccacggtc 360

gataaggtga?acaagcttct?caacgccacc?tttgcctact?accaaagcgg?ctcttcccag 420

agattgcgca?caacagagta?ctccatcccg?gatgatctgg?tcgactcaat?cgacctcatc 480

tccccaacga?ccttcttcgg?caaggaaaag?accactgctg?gtctgaacca?gcgggcgcaa 540

aagattgaca?cccatgtggc?caaacgctcc?aacagctcgt?cctgtgccga?tgtcatcacg 600

ctgtcctgcc?tgaaggagat?gtacaatttt?ggcaactaca?ctcccagcgc?ctcgtcgggc 660

agcaagctgg?gcttcggcag?cttcctgaac?gaatccgcct?cgtattctga?ccttgccaag 720

ttcgagaagc?tgtttaacct?gccctcccag?agcttttccg?tggagttggt?caacggcggt 780

gtcaatgatc?agaatcaatc?gacggcttcc?ttgaccgagg?cggacctcga?tgtggaattg 840

ctcgtcggag?ttgctcatcc?cctcccggtg?actgagttca?tcacttctgg?cgaacctgcc 900

gccgacaacg?agaacgagcc?ttacctccag?tactatgagt?accttctctc?caagcccaac 960

tcggctctgc?cccaagtgat?ttccaactcc?tatggtgacg?acgaacagac?cgttccagag 1020

tactacgcca?agcgagtctg?caacctgatc?ggacttgttg?gcctgcgcgg?catcagtgtc 1080

ctcgagtcgt?ccggtgacga?aggtatcgga?tctggctgcc?gaaccaccga?cggcaccaac 1140

cgaacccaat?tcaaccccat?cttcccggcc?acctgtccct?acgtgactgc?cgtgggagga 1200

acaatgtcct?atgcccccga?aatcgcctgg?gaagccagtt?ccggcggatt?cagcaactac 1260

ttcgagcggg?cgtggttcca?gaaggaagct?gtgcagaact?acctggcgca?ccacatcacc 1320

aacgagacca?agcagtacta?ctcgcaattc?gccaacttta?gcggtcgcgg?atttcctgac 1380

gttgctgccc?atagctttga?gccttcatat?gaggttatct?tctacggcgc?ccgctacggc 1440

tccggcggta?cctcagccgc?gtgtcccctt?ttctctgcgc?tagtgggcat?gctgaacgat 1500

gctcgtctgc?gggcgggcaa?gtccacgctg?ggtttcttga?accccctgct?ctatagcaag 1560

gggtacagag?cgttgactga?tgtgacgggg?ggccagtcga?tcggatgcaa?tggcattgat 1620

ccgcagaatg?atgagactgt?tgccggcgcg?ggcattatcc?cgtgggcgca?ctggaatgcc 1680

acggtcggat?gggatccggt?gactggattg?ggacttcctg?actttgagaa?gttgaggcag 1740

ttggtgctgt?cgttgtag 1758

<210>68

<211>798

<212>DNA

＜213〉aspergillus niger

<400>68

atgaagacta?ctgctctctt?gaccgccggc?ctgctggcca?ccactgctat?ggccgctcct 60

ctgacggcca?agcgccaggc?tgctcgggcc?aagcgctcca?cgaaccgcca?gagcaaccct 120

cccttcaagc?ctggcaccaa?cgaggtcctc?gcccttaacg?gcaccaagaa?tgtggagtac 180

agctccaact?gggccggtgc?cgtcctcatt?ggcactggtt?acactgccgt?gaccgccgag 240

ttcgtcgtgc?ccaccccctc?cgtgccctcc?ggtggctcga?gccgcgagga?gtactgtgcc 300

tccgcctggg?tgggcattga?cggtgacacc?tgtgacactg?ctatcctcca?gaccggtgtg 360

gacttttgtg?tccagggcag?cgaggtgagc?ttcgatgcct?ggtacgagtg?gtaccccgac 420

tacgcctacg?acttcagcgg?catctccatc?tcggccggtg?ataccatcaa?ggtcaccgtc 480

gatgccagca?gcgacaccac?cggtactgcc?acgattgaga?acgtgagcac?tggtaccacg 540

gtcacccaca?gcttcacggg?cggtgttgat?ggtgatctgt?gtgagtacaa?cgctgagtgg 600

atcgtcgagg?acttcgagga?ggatgactcc?ctcgttccct?ttgccgactt?tggcaccgtg 660

actttcacca?gctgctccgc?taccaaggat?ggttcctctg?ttggccctga?ggatgctacc 720

atcatcgaca?tcgagcagaa?tgaggtgctg?acctccgttt?ccgtctccag?tagcgaggtc 780

gttgtcaagt?acgtctaa 798

<210>69

<211>1743

<212>DNA

＜213〉aspergillus niger

<400>69

atggtcgcct?tttcccgcat?ctcggcaggc?ttcgcccttg?ccgcccctgc?cctggccagc 60

gtcgtcctgg?agaccgtcaa?gtctgttccc?agcgactgga?agctcgtgga?ggctgctgat 120

accagctcca?caatttcttt?gtccgttgct?ctggcgcgtc?agaacctgga?ccagttggag 180

gagaagctcc?tggccgtgtc?cacccctggc?aaggacacct?acggccagtt?cttggatctg 240

gacgacatca?atgagcagtt?tcctctcgca?gatgacgctg?ctgttgtggc?ttggctgaag 300

aaggcaggcg?tcacccagat?ccataaggag?ggtggtctgc?tgaactttgc?gaccactgtg 360

ggcacagcca?accagcttct?caacaccacc?ttctcggtgt?acaagagcgg?atctacccag 420

aagctgcgca?caacgcaata?ctctgttccg?gatgagctga?ccgggtccat?tgatctcatc 480

tcgccgactg?ttttctttgg?aaagtccaac?gctgcgcgct?cggcggccgt?gcgtgcttcg 540

cagactacca?aggagaccag?cagaaagaag?agcagtaatg?tgtgcgagta?catcactccg 600

gattgcctca?aagagcagta?tagcattgac?tatacgcccg?aggcatcgtc?gggaagtcgt 660

gttgggtttg?gcagtttctt?gaacgagtcg?gccttgtact?cggatttgga?tctgttcacc 720

cagtactttg?acattcccca?gcagagtttc?actgttgaga?ctatcaacgg?gggaatcaac 780

aaccaggaga?atgatccgga?tggtgaagcc?gatctcgatg?tccagaacat?cgtgggcatc 840

tcgcatccct?tgccggtgac?ggagtacatt?accggaggat?ctcctccatt?cattcccgac 900

gtcgagacta?ctaccgacga?gaacgagcct?tacctgcagt?actacgagta?tctgctggcc 960

aagaccaacg?acgagctgcc?actggttatc?agcaactcgt?acggcgatga?cgaagatacc 1020

gttcccattg?cctacgccac?ccgcgtatgc?aacctcatcg?gcctgatggg?cacacgtggt 1080

atctccatcc?tcgagtcttc?cggcgactct?ggtgtgggcg?gcgcatgcat?gtccaacgac 1140

ggcaccgaca?agaccgaatt?cacccccatg?ttcccaggaa?catgcccgta?catcaccgcg 1200

gtcggcggca?cccaagacgt?gcccgaagtc?gcctgggtgg?acagctccgg?cggcttcagc 1260

aactacttct?cgcagccgtc?gtaccagtcg?gatcaggtgg?agacctacct?ggacaagtac 1320

atctctgcct?cgacgaagaa?gtactacgag?cagtacacca?acttcagcgg?tcgcgcgttc 1380

cctgacgtgt?ctgcgtttgc?aggttctcct?tactacgaaa?cttatattga?tggtcagctc 1440

ggccttgtgg?cgggtacttc?tggcgctagc?cctgtgtttg?cggggatcgt?cgcgctgctg 1500

aacgatgccc?gtctgcgggc?caacaagaca?tccttgggct?tcctgaaccc?ttggctgtac 1560

tcgagcggct?acaagagcct?gaatgacatt?accagtggcg?aggcagtggg?ctgccaaggc 1620

gatgtggagg?gcgctggagt?cattccttgg?gcgagctgga?atgccacgac?gggatgggat 1680

ccggcgacag?ggctgggaac?gcctaatttt?gccaagctga?aggaggcggt?tcttgcgttg 1740

taa 1743

<210>70

<211>1896

<212>DNA

＜213〉aspergillus niger

<400>70

atgcatggtc?tgcgcctagt?atgcagcata?gggacattgc?ctttggttat?cctggcatat 60

ccggcggctt?cattgcatac?aacttcagca?gccgtggact?tggactccct?tcgtctgacc 120

tctaactccg?aatacgtcaa?ttctgtccat?gtagacacga?atcgatcagt?cgcagtgtcc 180

gctgaagaac?attataccga?tacagcagct?cgactggttc?agaacattgt?tcctggagcg 240

agctttcgtc?tcatcgatga?ccactttgtc?ggcgacaatg?gagttgcaca?tgtatacttc 300

cgccaaacgc?tccatggtat?tgacattgac?aatgcggatt?tcaatgttaa?tattggaaaa 360

gatggactgg?tcttgtcttt?cggacattcg?ttcttcacag?gcgcgttgcc?gagcagccat 420

ctggacaata?ccaacgtttt?gagtccggag?gctgcactta?gaggagcaag?ggacgctata 480

cagcttccac?tgactattga?caatgtttct?actgaagctg?cagaggggcg?gaacgagtac 540

atattcagag?aggcagtggg?agcggtatct?gaccccaaag?ctaagctagt?ctaccttgtc 600

aagccagaag?ggactctggc?gctcacctgg?aggatagaaa?cagacatgta?tgagcactgg 660

ctactgacat?acattgatgc?agagactacc?actgtccacg?gcgtggttga?ctatgtcgca 720

gacgcgacat?atcaagttta?tccctggggc?acaaacgatc?cagcagaagg?acatcgcacc 780

attgtcaccg?acccctggga?cctatccgca?tccgcataca?cctggataag?cgatggacgg 840

gacaactaca?ccacaaccag?aggcaacaat?gccatcgcac?actggaatcc?gaccggcggt 900

ggctcctatc?tctacaacct?acgtccatcc?gaccccaact?tgaatttcca?atggccatac 960

tccccaaaca?tgtccccacc?ccgatcatac?atcaacgcct?ccatcgtcca?actcttctac 1020

acagcaaacg?cctaccacga?cctcctctat?acactcggct?tcaccgaatc?cgctggcaac 1080

ttccaatgga?ataacagcgc?ccacggcggc?cgagacaaag?actacgtgat?cctcaacgca 1140

caagacggct?ccgggttcag?caacgcaaac?tttgcaaccc?cacccgatgg?tatccccggc 1200

cgtatgcgca?tgtacatctg?gatcgagtct?actccgtcgc?gtgatggaag?ttttgacgcg 1260

ggcattgtaa?ttcacgaata?cactcacggt?gtatccaatc?gtctcaccgg?cggctcccac 1320

aacgccggat?gcctcagcgc?cctcgaatcg?ggtggcatgg?gcgaaggctg?gggcgacttt 1380

atggcgacgg?ccatccgaat?caagcccaac?gatacacgca?caacgtctta?cactatgggt 1440

gcatgggcag?ataatgataa?atgtggtgtc?cgggactatc?cttattctac?ctcctttact 1500

gagaaccctt?tgaactatac?gagcgtgaat?accatgaacg?gcgtgcacgc?catcggaact 1560

gtctgggcaa?ccatgctata?cgaggtcttg?tggaacctca?tcgacaagta?cgggaagaat 1620

gatgggtcga?ggccggtgtt?tagaaacggg?gtgcctacag?atggaaagta?cttgatgatg 1680

aagttggtgg?tggatgggat?ggcactgcaa?ccatgtaatc?cgaacttcgt?gcaagccagg 1740

gacgcgatcc?ttgacgcaga?cattgtgttg?actggcggga?agaatcgctg?tgagatctgg 1800

agggggtttg?cgaagagagg?attggggcaa?ggagcggctc?atagtagttt?aaattggatg 1860

cggaggggga?gtacacttct?tcctacggga?tgttag 1896

<210>71

<211>1185

<212>DNA

＜213〉aspergillus niger

<400>71

atggtcgtct?tcagcaaaac?cgctgccctc?gttctgggtc?tgtcctccgc?cgtctctgcg 60

gcgccggctc?ctactcgcaa?gggcttcacc?atcaaccaga?ttgcccggcc?tgccaacaag 120

acccgcacca?tcaacctgcc?aggcatgtac?gcccgttccc?tggccaagtt?tggcggtacg 180

gtgccccaga?gcgtgaagga?ggctgccagc?aagggtagtg?ccgtgaccac?gccccagaac 240

aatgacgagg?agtacctgac?tcccgtcact?gtcggaaagt?ccaccctcca?tctggacttt 300

gacaccggat?ctgcagatct?ctgggtcttc?tcggacgagc?tcccttcctc?ggagcagacc 360

ggtcacgatc?tgtacacgcc?tagctccagc?gcgaccaagc?tgagcggcta?cacttgggac 420

atctcctacg?gtgacggcag?ctcggccggc?ggagacgtgt?accgggatac?tgtcactgtc 480

ggcggtgtca?ccaccaacaa?gcaggctgtt?gaagcagcca?gcaagatcag?ctccgagttc 540

gttcagaaca?cggccaatga?cggccttttg?ggactggcct?ttagctccat?caacactgtc 600

cagcccaagg?cgcagaccac?cttcttcgac?accgtcaagt?cccagctgga?ctctcccctt 660

ttcgccgtgc?agctgaagca?cgacgccccc?ggtgtttacg?actttggcta?catcgatgac 720

tccaagtaca?ccggttctat?cacctacacg?gatgccgata?gctcccaggg?ttactggggc 780

ttcagcaccg?acggctacag?tatcggtgac?ggcagctcca?gctccagcgg?cttcagcgcc 840

attgctgaca?ccggtaccac?cctcatcctc?ctcgatgacg?aaatcgtctc?cgcctactac 900

gagcaggttt?ctggcgctca?ggagagcgag?gaagccggtg?gctacgtttt?ctcttgctcg 960

accaaccccc?ctgacttcac?tgtcgtgatt?ggcgactaca?aggccgttgt?tccgggcaag 1020

tacatcaact?acgctcccat?ctcgactggc?agctccacct?gctttggcgg?tatccagagc 1080

aacagcggtc?tgggactgtc?catcctgggt?gatgttttct?tgaagagcca?gtacgtggtc 1140

ttcaactctg?agggccctaa?gctgggattc?gccgctcagg?cttag 1185

<210>72

<211>1197

<212>DNA

＜213〉aspergillus niger

<400>72

atgaagtcag?cctccttgct?cacagcatcc?gtgctgttgg?gctgtgcctc?cgccgaggtt 60

cacaagctca?agcttaacaa?ggtgcctctg?gaagagcagc?tttacacgca?taacatcgac 120

gcccatgtcc?gcgctctggg?ccagaagtac?atgggtatcc?gcccgtccat?ccacaaagag 180

ctggtcgagg?agaaccctat?caatgacatg?agccgtcatg?atgttctggt?ggacaacttc 240

ctgaacgcac?agtacttctc?tgagatcgag?ctgggtactc?ccccccagaa?gttcaaggtt 300

gtcctggaca?ctggcagctc?gaacctttgg?gttccttcga?gcgaatgcag?ctctatcgcc 360

tgctacctcc?acaacaagta?tgattcgtct?gcctccagta?cgtatcacaa?gaatggcagt 420

gaattcgccg?tcaagtacgg?ctctggcagc?cttagcggat?tcatttctca?ggacaccctg 480

aagattggcg?acctgaaggt?caagggacag?gacttcgctg?aggcgaccaa?tgagcctggc 540

cttgcctttg?ccttcggccg?gttcgatggc?attctcggct?tgggttatga?caccatctcc 600

gtgaacaaga?ttgttcctcc?cttctacaac?atgcttgacc?agggactcct?cgacgagccg 660

gtctttgcct?tctaccttgg?agataccaac?aaggagggtg?acgagtccgt?ggcgaccttc 720

ggtggtgtcg?acaaggacca?ctacaccggc?gagctgatca?agattcccct?ccgtcgcaag 780

gcttactggg?aggttgagct?tgacgccatt?gctcttggcg?atgatgttgc?tgagatggag 840

aacaccggtg?tcattctgga?cactggtacc?tccctgattg?ctctgcctgc?tgacctggct 900

gagatgatca?atgctcagat?cggtgctaag?aagggctgga?ccggccagta?caccgttgac 960

tgcgacaagc?gctcgtccct?gcccgatgtt?actttcaccc?ttgccggcca?caacttcacc 1020

atctcctcgt?atgactacac?cttggaggtg?cagggctctt?gcgtcagtgc?cttcatgggc 1080

atggacttcc?ctgagccggt?tggtcccttg?gccattttgg?gcgatgcgtt?cctgcgcaag 1140

tggtacagcg?tgtatgacct?gggcaacagc?gctgttggtc?tggccaaggc?caagtaa 1197

<210>73

<211>1182

<212>DNA

＜213〉aspergillus niger

<400>73

atgcgcaagt?accgcttcca?tcccaccaag?cctggtccct?acactctcag?cagctccatc 60

caacagaccg?gtcgtccgta?cactgaaaag?cccatcgggg?gtcgggccca?tatccggcag 120

ctggtgcgga?agaagagcac?caccagcgat?gaggttggcg?aggttccggc?cgaagatgtg 180

cagaacgact?ccatgtatct?ggcgaccgtg?gggatcggaa?ccccggcgca?gaacctgaag 240

ttggactttg?acactggttc?agctgatctt?tgggtctggt?ccaacaaact?cccctcaacc 300

cttctatccg?agaacaagac?ccatgcgatc?ttcgactcgt?ccaaatcgag?caccttcaag 360

accttggaag?gtgaatcctg?gcaaatctcc?tacggagatg?gatcctccgc?atcagggagt 420

gtgggcaccg?acgacgtcaa?cattggcggc?gtagtcgtca?agaaccaagc?cgttgagctg 480

gcagagaaga?tgtccagcac?attcgcccaa?ggcgaagggg?acggattgct?cggtctagca 540

ttcagcaaca?tcaacacggt?acagccaaag?tccgtgaaaa?cgcccgtcga?gaacatgatc 600

ctgcaggatg?acattcccaa?gtcggctgag?ctgttcacgg?ccaagctgga?tacctggcgg 660

gacactgatg?acgagtcgtt?ttacaccttt?ggcttcattg?accaggatct?ggtgaagacg 720

gcaggtgaag?aggtctacta?cacccctgtc?gataacagtc?aaggcttctg?gctattcaac 780

tcgacctccg?cgacggtaaa?tggaaagacc?attaaccggt?cgggtaacac?cgccattgct 840

gataccggta?cgacgctggc?cttggtggac?gatgacacgt?gtgaggccat?ttatagtgca 900

attgacggcg?cctattatga?tcaggaagta?cagggctgga?tctatccgac?cgatacggcg 960

caggataagc?tacccactgt?gtcgtttgcc?gtgggtgaaa?agcagttcgt?ggtgcagaag 1020

gaggacctgg?cgttttcgga?ggcgaagacg?ggctatgtct?atggaggaat?ccaaagtcgt 1080

ggtgatatga?ccatggacat?cttgggagac?acatttttga?agagtattta?tgctgtaagt 1140

gcattgctgt?tggcgttaag?gggtgatatc?gaagctcact?aa 1182

<210>74

<211>849

<212>DNA

＜213〉aspergillus niger

<400>74

atgaagttct?ctaccatcct?taccggctcc?ctcttcgcca?ctgccgctct?ggctgctcct 60

ctcactgaga?agcgccgtgc?tcgcaaggag?gcccgcgccg?ctggcaagcg?ccacagcaac 120

cctccctaca?tccctggttc?cgacaaggag?atcctcaagc?tgaacggcac?ctccaacgag 180

gattacagct?ccaactgggc?tggtgccgtc?ctgatcggcg?acggctacac?caaggtcact 240

ggcgagttca?ctgtccccag?tgtctctgct?ggatctagca?gctccagtgg?ctacggcggt 300

ggctacggct?actacaagaa?caagagacaa?tccgaggagt?actgcgcctc?cgcttgggtt 360

ggtatcgacg?gtgacacctg?cgagaccgct?attctccaga?ctggtgtcga?cttctgctac 420

gaggatggcc?agacttccta?cgatgcctgg?tacgagtggt?accccgacta?cgcctacgac 480

ttcaacgaca?tcaccatctc?cgagggtgac?accatcaagg?tcactgtcga?ggccaccagc 540

aagagcagcg?gtagcgccac?cgttgagaac?ctgaccactg?gccagtccgt?cacccacacc 600

ttcagcggca?acgtcgaggg?tgacctttgc?gagaccaacg?ccgagtggat?cgtcgaggac 660

ttcgagtctg?gtgactctct?tgtggctttc?gctgacttcg?gctccgttac?cttcaccaat 720

gctgaggcta?ccagcgacgg?ttccactgtc?ggcccctctg?acgctaccgt?tatggacatt 780

gagcaggatg?gcaccgtcct?caccgagacc?tccgtctctg?gcgacagcgt?cactgtcacc 840

tacgtttaa 849

<210>75

<211>822

<212>DNA

＜213〉aspergillus niger

<400>75

atgggagatt?acggccccgg?agtgtcgtca?ctcacggcac?agctacctgg?aaatccgcct 60

gtctctgaaa?cagatcagga?tgagatctca?gtacttgtaa?cgggctttgg?gccattcaag 120

tctaatctag?tgaacgcctc?atatctgata?gcctcgtccc?taccaccctc?tttcacattc 180

tcacctgcat?cttcagacgg?ctctgatgct?gttccccgtc?gagtttcgat?aaatgtccat 240

ccttcaccca?tacccgttgc?atattcatcg?gtgcggacga?ccctccccgt?cattctcgat 300

gactatgcca?agacgcacgg?aggccgacgc?ccagacatcg?tcatacacat?tggcatagca 360

gcaatgagga?actactattc?cgtggagacg?caggctcacc?gtgatgggta?tctgatgtcc 420

gacatcaaag?gcagatccgg?gtacgaggat?ggcgagaagc?tgtggaggga?gctcgacttg 480

ccactggtgc?ttagggctgg?cccttcagag?ggacacgcct?cggagaagaa?acatctcagc 540

ccccgtccac?cggacgaaga?tttcctagca?gcatggaaga?cattttgccc?tccagaaacc 600

gatgcgcgga?tctccactga?tgccggacgt?tatctctgcg?agttcatcct?gtacaccagc 660

ttggcactgg?cataccaggc?gggtgaggat?cgcaatgtca?ccttcttcca?tgttcccgcg 720

tcatgcttgg?atgaggatat?agagacgggc?aaggaggttg?ccgtcgcgct?aatcaaggct 780

cttgtgacta?gctggagtga?gcagcagcac?agcgttccct?ag 822

<210>76

<211>1629

<212>DNA

＜213〉aspergillus niger

<400>76

atgggctcaa?ggcagggaaa?ggcccccttt?ggctggggta?ctcagtcact?tgctcacttt 60

ggtatcaacc?cagaccttgg?gttgcacaac?cagcagaacc?tcaactccct?catttcacat 120

tcagcgatgg?ccactgcgtt?ggagacggaa?tatgccacca?tccctattga?ccataacaac 180

gcatcggctg?gcacttatca?aaatcggttc?tgggtcagcg?atgaattcta?tcagcctggc 240

aacccgatat?ttgtgtacga?taccggggag?tcggatggcg?gatcgatagc?ccagtcctac 300

ctaacctcca?ctctctcctt?cttcagagaa?ttcctgatcg?aattcaacgc?catgggaatc 360

gcctgggagc?acagatacta?tggaaactcg?accccggctc?ccgtatccta?tgaaactcca 420

cccgaggcat?ggcaatacct?caccaccaag?caggcgctcg?cggaccttcc?gtactttgct 480

agtaacttta?gccgcgagaa?gtatcctgac?atggacctga?cgccgcaggg?cacgccgtgg 540

atcatggtgg?gcggctcgta?cgcagggatt?cgtgctgcat?taactcgcaa?ggagtaccca 600

gagacgatat?tcgcagcctt?ttcctcatcg?tctccggtgg?aagcacaggt?caatatgagc 660

gcgtattacg?accaagtcta?tcgtggcatg?gttgccagcg?gatggaccaa?ctgctcggca 720

gatatccacg?ctgctctgga?atatattgac?gatcaacttt?cggatgaaga?tacagctacc 780

tcggtcaaac?aacttttctt?cggatctggc?gccgagacca?actccaacgg?tgatttcact 840

gcagcgctaa?ctgccatcta?cggctacttc?caaagttatg?gtatggcggg?aggtattgga 900

ggtctaggcg?cattctgcga?gtatctcgaa?attgatccca?agacgaacgg?gactacagga 960

ccggatggcc?ttgcccctac?gtatggcggc?cagtatgtcg?ccgaacgatg?ggccgcatgg 1020

ccaacctttc?tcgagctggt?caatctgaat?atggggacca?actgcgggcc?tcaggacgcg 1080

tctcagccaa?ttgactgtga?cttttccaag?ccatacggcg?atccctcggc?catcacttgg 1140

acttggcaat?actgcagcga?atgggggttc?ttccaggcga?acaacgatgg?gccgcactcg 1200

ctggcctcgc?gatatcagtc?ggtggaatac?cagcaagaag?tatgtaaccg?gcagttcccc 1260

gatgcagtgg?acaagggact?gctgcctccg?tcgccgcggg?cggatgatgt?caaccaagag 1320

tttgggggat?ggacgatccg?cccgtccaat?gtttacttca?gcggaggaga?attcgatccg 1380

tggcgatcat?tgtccattct?gtcgacagaa?gatttcgcac?ctcaaggggt?ggagtttacg 1440

agcgcgatcc?cagcctgtgg?ggtgcagacc?aatgaggaca?ccgtctttgg?atacgtcatg 1500

cagaactcgg?aacattgctt?tgactttcaa?gcgacgccga?ccgtggggaa?gttatcacgc 1560

ggcatcttca?catccgcctt?gttgcaatgg?ctcgaatgtt?ttggacagaa?ctcaagccaa 1620

tccaggtga 1629

<210>77

<211>1176

<212>DNA

＜213〉aspergillus niger

<400>77

atgaagctct?caatagctct?tgcactcggc?gcaacggctt?cgacgggggt?gttggctgct 60

gttgtaccgc?agcaagaacc?gctgataacc?ccccaagatc?ccccaactca?tcatcatcag 120

gagaagttct?tgatcgagtt?ggctccttat?cagacgagat?gggttaccga?ggaagaaaag 180

tgggacttaa?aactggatgg?cgtgaacttc?atcgatatta?ctgaagaacg?aaacactggg 240

ttctacccaa?cgttgcatgc?tggtagctat?gttcactatc?cgccgacgat?gaagcatgcg 300

gagaaggtgg?ttccccttct?gcggggtctc?tccaaggaca?acatggagca?aaacctcaac 360

aaatttacct?catttcacac?tcgctactat?aggtcgtcca?ctggtattga?gtccgcaaag 420

tggctataca?gtagggtttc?ggatgtcatt?gagcagtcgg?gtgcagcaga?gtacggcgcc 480

actgtggagc?agttcgctca?ctcatggggc?caattcagta?tcattgctcg?gatcccaggc 540

cagactaaca?aaactgttgt?cctgggcgca?catcaggaca?gcatcaatct?tttcctcccc 600

tccatcctag?ctgcacctgg?tgccgatgat?gacggaagtg?gaaccgtgac?tatactcgaa 660

gctttgcgtg?gtctgctgca?gtcagacgcc?attgtccggg?gcaacgcttc?caacacaatc 720

gaattccact?ggtactcggc?agaggaaggt?ggtatgcttg?gttcgcaagc?catattctct 780

caatataaga?gagataagcg?agacatcaag?gcgatgcttc?aacaggatat?gactggttat 840

acccagggag?ctctggacgc?cggtcgtcaa?gaagccattg?ggattatggt?tgactacgtt 900

gatgagggac?tgacacaatt?cctcaaagat?gtcactactg?agtattgtgg?tattggctac 960

atcgaaacca?gatgtggcta?cgcctgttcg?gaccacacgt?ccgcaagcaa?atatggctat 1020

cccgcagcta?tggcgacgga?atccgaaatg?gaaaacagca?acaagaggat?ccacacgact 1080

gatgacagca?tccggtatct?aagcttcgat?catatgctgg?agcatgcgag?gttgacactt 1140

ggcttcgctt?acgagctggc?ctttgctcaa?ttctag 1176

<210>78

<211>1329

<212>DNA

＜213〉aspergillus niger

<400>78

atgagaacta?ctacgtcttt?tgctaggctt?gcattggcag?tggcctcagt?tggtattgtc 60

tttgctagtc?caacaaaaaa?taacgatggg?aaactggtat?atggctcacc?agaatccgtc 120

ggcatgatat?ccgccccttt?gcaccaaatg?gtccaaaatg?ttagcgcata?tacacatgct 180

gccaactata?gcaagttctc?gtacgacaaa?gtccatccca?tcgagccagg?gtctgttacc 240

ctggtggctc?tcgacggtgt?catcgtcagc?gaatttgcct?tgggcaagag?aaatctctac 300

gccgatgtca?acggcaccaa?tttacctcga?tacctgcagg?aagacaccac?cctggataca 360

gtctacgata?tggcaagcct?cacgaagctg?ttcaccacgg?tagctgcttt?acgggaactt 420

gacgctggtc?gaattgcgct?taatgtaact?gttgcaactt?atataccgga?ctttgcgacg 480

aatgggaagg?agaatattac?tatcttggag?ctgttcacgc?atacaagcgg?tttcgcttct 540

gatccatcgc?caccactttt?ctctgcttat?tatacgacgt?atgatgaacg?cattaaagca 600

attttgacgc?aaaaaattat?caataccccc?ggcagcacat?acctctactt?agatctcaac 660

tttatgtcgc?tgggcctcgt?tatcgagacc?gtaacgggac?gtgccctgga?tgatcttatt 720

tatgacttca?ccagaccgct?tgaaatgaca?tctaccttct?tcaaccgcgg?gaatatcgaa 780

ggctctacac?cccagtcacc?caactacgac?cgcacagccg?tacaagaatt?tcagatcgca 840

gccctcggac?cctcagaacc?acagcgtcca?caaccagtgc?gcggcacagt?tcacgacgag 900

aacgcatggt?ccctagacgg?cgtatcaggt?catgcaggtc?tattctccac?tgtgcgcgat 960

acagcgacat?tctgccagat?gatcctcaac?aacggcacat?atgcaggcca?acggatcctt 1020

tctcgaacag?cggtagacat?gattttcaca?aacttcaatg?ccaggtttcc?gggggatgct 1080

cgtagtttag?ggtttgagtt?ggatcagtat?tctactgcgg?gaccgatggc?gagtttgcaa 1140

actgcgagtc?acactggatt?tactgggact?acgttggtga?tggataggac?gtataacgcc 1200

ttttggttgc?attttagtaa?ccgggtgcat?ccgtctaggg?catggtctag?caatactatt 1260

gtgagagagg?ctattgggta?ttgggttggg?aagagcttgg?ggttggatgt?tgcgtttgct 1320

ctgttgtaa 1329

<210>79

<211>1839

<212>DNA

＜213〉aspergillus niger

<400>79

atggcgtcct?ggttgctctc?gacgctcctt?tttctgagcc?cgtccttggt?gtcagccaaa 60

tcggccgcag?actattatgt?tcactccttg?cccggtgccc?ccgaggggcc?cttgctgaag 120

atgcatgccg?gccatattga?ggtggatcca?cagaacaatg?gaaatctttt?cttctggcac 180

taccagaatc?gccatattgc?caaccgccag?cggactgtga?tctggttgaa?cggtggtccc 240

ggatgtagtt?ccatggacgg?cgcgttgatg?gaggtcggtc?cgtatcgcct?gaaggacaat 300

gaaaccttga?cctataatga?gggttcctgg?gacgaattcg?ccaatttgtt?gttcgtcgat 360

cagccagtcg?gaaccgggtt?cagttatgtc?aacacggaca?gctatcttca?tgagctcgat 420

gagatgtcgg?ctcagttcat?tgtctttctg?gaagagtggt?tcagattatt?tccggagtat 480

gaacgcgatg?atatctacat?tgccggcgag?tcttacgccg?gtcagcatat?tccatacatc 540

gccaaagcca?tccaggaacg?gaacaagaac?gttcaaggga?agaccatcgc?ttcgtggaat 600

ctaaaaggcc?tattgattgg?caatggttgg?atttctccta?atgaacagta?catgtcctac 660

ttgccctacg?catatgaaga?aggccttatc?aaggaaggca?gccggaccgc?gaaggaactc 720

gaagttttac?agtcagtctg?taagtccagg?ctggaaactg?gcaagaacaa?ggtccacctc 780

aacgactgcg?agaaggtcat?gaatgctctg?ttggataaga?cggtcgaaga?caacaaatgt 840

ctcaacatgt?atgacatccg?ccttcgtgac?accaccgatg?catgcggtat?gaactggccc 900

accgacctgg?aggacgtgaa?gccctatctg?cagcgggaag?atgtggttaa?agcgcttaac 960

atcaatccgg?agaagaagtc?tggctgggtg?gagtgttcag?gtgcagtgag?cagcgctttc 1020

aatccgcaaa?agtccccgcc?ctcggttcaa?ctacttcccg?gcttgctgga?atcgggactt 1080

caaatcctcc?ttttcagcgg?agacaaggac?ctgatttgca?accatgttgg?aacggaacag 1140

ctcatcaata?acatgaagtg?gaacggaggc?acgggtttcg?agacctcacc?tggcgtctgg 1200

gctcctcgac?acgactggag?tttcgaaggc?gagccggcgg?gtatctatca?atatgccaga 1260

aacctgactt?acgtgctcat?ctacaacgca?agccatatgg?ttccctacga?ccttcctcgt 1320

cagagccggg?acatgctaga?tcgcttcatg?aatgtcgata?tcgcgagcat?cggaggcagc 1380

cccgccgact?cgcgcattga?cggcgagaag?ctgccccaga?cgtcggtggg?cggccatccc 1440

aacagcaccg?cggcggagga?gcaggagaag?gagaggatca?aggagacgga?atggaaagcc 1500

tacgccaagt?caggcgaagc?cgttctcctc?gtcgtcatta?tcggtgtatt?agtttggggc 1560

ttcttcatct?ggcgcagccg?ccggcgtcac?cagggatacc?ggggcgtctg?gcataaggac 1620

atgagcggaa?gctctgttct?cgagcggttc?cacaacaagc?gcacgggagg?cgcagacgtc 1680

gaagcggggg?atttcgacga?ggcggagctc?gatgaccttc?attctccaga?cctcgaaaga 1740

gaacactacg?ccgtgggcga?ggacagcgac?gaggatgata?tttcacgaca?gcattctcaa 1800

caggcctccc?gagccggggg?cagtcataat?ctatcctag 1839

<210>80

<211>1596

<212>DNA

＜213〉aspergillus niger

<400>80

atgtttctga?tttcacctgc?agtgacagtt?gcggctgcac?ttctgctgat?caacggcgca 60

ggagcaactc?aatctgaacg?aagtcgggct?gccgctcatt?tttccaaacg?tcatccgacg 120

taccgtgctg?cgaccagagc?ccagtcgagc?aacacttccg?actaccgatt?cttcaataat 180

aggaccaagc?cccacttggt?ggaaagctta?cccgatgtgc?acttcgatgt?tggggagatg 240

tactcggggt?cgatccctat?cgatgacagc?aacaatggat?ctcgatccct?gttttatatc 300

ttccaaccta?agataggcga?accttcagac?gaccttacca?tttacctcaa?tggagggcca 360

ggctgttcct?ccgaacaggg?attctttcag?gaaaatggca?ggttcacatg?gcagcctggt 420

acctatgcac?ccgtcatcaa?cgaatattct?tgggtcaatt?tgacgaacat?gctatgggtt 480

gaccaaccag?tcggaaccgg?attttccgtt?ggaaatgtta?cagccaccaa?cgaagaagag 540

attgccgccg?attttctcga?cttctttgaa?aagtttgaag?atctatacgg?gataaagaac 600

tttcgcattt?tcatgaccgg?tgagagctac?gccggtcgct?atgttcccta?tatctcgtcg 660

gcaatgctag?acaagaacga?caccacgcgt?ttcaatctga?gcggagccct?tctttatgac 720

gcctgcatcg?gccaatggga?ctacatccag?gccgaactcc?ctgcctaccc?cttcgtcaag 780

cagcacgctt?cactattcaa?cttcaatcag?tcctacatga?acgagcttga?aaccacctac 840

gaagaatgcg?gctacaaggc?ctacttcgat?gagtactttg?cctttccacc?aagcggcatc 900

caacccccaa?aatacatgaa?ctactccgag?tgcgacatct?ataacatgat?ctactacgaa 960

gcctataacc?cgaacccatg?cttcaatccc?taccgcgtca?ttgatgagtg?tccacttctc 1020

tgggacgtcc?tgggctggcc?gacagacttg?gcatacgagc?ctgcgcccac?cacatacttc 1080

aaccgtatcg?atgtcaagaa?ggccctgcac?gcccccatgg?atgtggaatg?ggagctctgc 1140

agctacgacc?tcgtcttcgc?tggaggcgac?gctgacccgg?gtccggagca?gcaaggggat 1200

gactcaccca?accccaccga?gggtgtcctc?ccgcgtgtta?ttgaggcgac?caaccgcgtg 1260

ctcattgcca?acggtgactg?ggactacctg?attatcacca?acggcaccct?cctcgccatc 1320

cagaatatga?cctggaacgg?ccagctgggc?ttccagtccg?cacctgccac?accgatcgat 1380

attcagatgc?ccgatctcca?gtgggttgag?atttttgagg?cccaggaggg?atatggaggg 1440

ctggatggcc?ctcagggggt?tatgggtgta?caacattatg?agcgcggttt?gatgtgggcg 1500

gagacatatc?agtcggggca?taagcaggct?caggatcagg?gccgtgtctc?gtatcgccat 1560

ctgcagtggc?tgttggggca?agttgagatt?ctttag 1596

<210>81

<211>1596

<212>DNA

＜213〉aspergillus niger

<400>81

atgctgtttc?gcagtctgtt?gtcgacggct?gtcctagccg?tctcgctgtg?cacggataat 60

gcttcagctg?ctaaacatgg?tcgatttggc?caaaaagctc?gcgacgccat?gaacatcgcg 120

aagcgttccg?ctaacgccgt?gaaacactcg?ttgaagatcc?ctgtcgagga?ctatcagttc 180

ttgaacaaca?agactaagcc?ttaccgcgtg?gaaagcctgc?ctgatgttca?cttcgatctg 240

ggcgagatgt?attccggctt?ggtccctatt?gagaagggca?acgtgtcacg?gtcccttttc 300

tttgtcttcc?agcccactat?tggcgagcct?gtggatgaga?tcaccatctg?gctgaatggt 360

ggccctggtt?gcagttccct?tgaggccttt?ctccaggaga?atggtagatt?cgtgtggcag 420

cctggaacct?accagcctgt?tgagaaccca?tactcgtggg?tgaatctcac?caatgttctg 480

tgggttgacc?aacctgtggg?aacgggattc?tctctgggtg?tcccaaccgc?tacgtccgag 540

gaggagattg?ctgaagactt?tgtgaagttc?ttcaagaact?ggcagcagat?ctttgggatc 600

aaaaacttca?agatctatgt?tactggagaa?agttatgcgg?gccgttatgt?tccttacata 660

tccgctgctt?tcctagatca?gaatgataca?gaacacttca?acctaaaagg?tgcactggca 720

tatgatccct?gtattggtca?gtttgactac?gtgcaggagg?aagcacctgt?tgttcccttt 780

gtccagaaga?acaatgccct?cttcaatttc?aatgcaagct?ttttggcgga?actagagagc 840

atccatgagc?aatgtggata?caaggatttc?atcgaccagt?atctagtctt?cccagcatcc 900

ggtgtccagc?cgccaaaggc?tatgaactgg?agcgatccca?cctgtgatgt?ttatgacatc 960

gttaataacg?ccgtcctgga?tcccaacccg?tgcttcaacc?cctacgaaat?caacgagatg 1020

tgccccattc?tctgggacgt?tcttggattc?cccaccgaag?tcgactatct?ccctgcgggc 1080

gccagcatct?actttgaccg?cgctgatgtt?aagcgtgcca?tgcacgctcc?taacatcacc 1140

tggtccgagt?gctcggtgga?gagcgtcttt?gtcgggggcg?acggcggtcc?cgagcaggag 1200

ggcgactact?cggccaaccc?catcgagcat?gtcttgcccc?aggtcatcga?aggcaccaac 1260

cgagttctga?tcggtaacgg?tgattatgac?atggtcatcc?ttaccaacgg?cacccttctc 1320

tcgatccaga?acatgacatg?gaatggaaag?cttggattcg?acacggcccc?cagcaccccc 1380

atcaacatcg?acatccctga?cctgatgtac?aatgaagtgt?tcattgagaa?cggctatgac 1440

ccacaaggtg?gtcagggtgt?catgggcatc?cagcactatg?agcgtggtct?tatgtgggct 1500

gagaccttcc?agagcggaca?catgcagccc?caattccaac?ccagagtgtc?ataccgtcac 1560

cttgagtggc?tgcttggccg?gcgggatacc?ctgtaa 1596

<210>82

<211>1479

<212>DNA

＜213〉aspergillus niger

<400>82

atgaaaggtg?cggcgctaat?tcctcttgcg?gcgggcattc?cttttgccca?tggcctgtct 60

ctccataaac?gcgacgggcc?tgccgtcgtt?cgtatgccca?ttgagcgcag?gagcgcccag 120

tccttgcaga?aacgagattc?tacggtcggt?gtgactttgc?agaactggga?tgcgacctat 180

tacgcagtca?acctgacgtt?aggaacacct?gcgcaaaagg?tatcattagc?tttggacact 240

ggcagcagcg?acctctgggt?gaacaccggc?aactcaactt?actgctcaat?cgacaatcta 300

tgcacccctt?atggcttgta?caatgccagc?gaatcgtcta?ctgtaaagac?cgtgggcaca 360

cacctcaacg?atacatatgc?ggacggcaca?aacctttacg?gtccttatgt?gaccgataag 420

ctcacgatcg?gcaacacaac?aatcgataat?atgcagtttg?ggatcgccga?gtcaacgact 480

agtaaacgcg?ggatcgccgg?cgtcggttac?aagatttcga?cctaccaagc?cgagcatgac 540

gacaaagtct?acgccaacct?ccctcaggcc?ctcgtcgaca?gcggtgccat?taagtctgct 600

gcgtacagca?tatggctaga?tagtttggag?gcgtcgactg?gctccctcct?tttcggaggt 660

gtcaatacag?ccaagtacaa?gggcgatctg?cagactcttc?cgatcattcc?tgtgtatggc 720

aaatactact?ccctcgccat?cgcccttacg?gagctcagcg?ttgcgaccga?ctccaactcc 780

agtagcttca?ccgacagtct?ccccctctct?gtgtcactcg?atactggcac?caccatgacg 840

gcactgccca?gcgacctggt?caacaaggtc?tacgatgcgc?tcaacgcaac?ctacgacaag 900

acatacgaca?tggcctacat?cgactgcgac?actagagagg?cggattacaa?tgtaacatac 960

agtttctccg?gggcaacgat?caccgtgagc?atgagtgagc?tgattatccc?cgcaacggag 1020

ccggggtggc?ccgacaacac?gtgtgtcttg?ggcctcgtgc?ctagccagcc?gggcgtgaac 1080

ctgctcggtg?atacattcct?gcgcagtgcg?tacgtcgtgt?atgatctcga?gaacaacgaa 1140

atctctctcg?ccaataccaa?tttcaatcca?ggcgacgatg?atatcctcga?aatcggaacg 1200

ggaacgtctg?ctgtgccagg?agccacaccg?gttccctctg?ctgtctcttc?tgcaactgga 1260

aatggactga?tctcgtctgg?caccgcagtg?cccacgctgt?cgggtgtcac?aataactgct 1320

acagccacag?caaccggctc?aaccggcact?ggctctagcg?gtggttcgtc?ggctgaagcc 1380

acgagtactt?cctcggaggg?cgctgcggcg?caagctacga?gcaacccgat?gaacctgctc 1440

ccaggacttg?cgggtatcgg?cctacttctc?gctctgtaa 1479

<210>83

<211>1836

<212>DNA

＜213〉aspergillus niger

<400>83

atgctgtcgt?ctctccttag?ccagggagca?gccgtatccc?tcgcggtgtt?gtcgctgctc 60

ccttcgcctg?tagccgcgga?gatcttcgaa?aagctatccg?gcgtccccaa?tggctggaga 120

tacgccaaca?atcctcaagg?caacgaggtc?attcgcttgc?aaatcgccct?tcagcagcat 180

gatgtcgctg?gtttcgaaca?agccgtgatg?gatatgtcca?cccccggaca?cgccgactat 240

ggaaagcatt?tccgcaccca?cgatgagatg?aagcgcatgt?tgctccccag?cgagactgcc 300

gtcgactcag?tccgcgactg?gctggaatcc?gccggtgtcc?acaatatcca?ggtcgacgcc 360

gactgggtca?agttccatac?caccgtaaac?aaggccaatg?ccctgctgga?tgccgacttc 420

aagtggtatg?tcagcgacgc?caagcatatt?cgtcgtctgc?gcaccctgca?atactccatc 480

cccgacgccc?tggtctcgca?catcaacatg?atccagccca?ccacccgctt?tggccagatc 540

cagcccaacc?gtgccaccat gcgcagcaag?cccaagcacg?ccgatgagac?attcctcacc 600

gcagccaccc?tggcccagaa?cacctcccac?tgcgactcca?tcatcacacc?gcactgtctg 660

aagcagctgt?acaacatcgg?tgactaccag?gccgatccca?agtccggcag?caagatcggc 720

tttgccagct?accttgagga?atacgcccgg?tatgccgatc?tcgagaggtt?cgagcagcac 780

ctggctccca?atgccatcgg?ccagaacttc?agcgtcgtcc?aattcaacgg?cggcctcaac 840

gatcagcttt?catcgagtga?cagcggcgaa?gccaacctcg?acctgcagta?catcctgggc 900

gtcagcgctc?ccgtccccat?caccgagtac?agcaccggcg?gacgcggcga?actagtcccc 960

gacctgagct?cccccgaccc?caacgacaac?agcaacgagc?cctaccttga?cttccttcag 1020

ggaatcctca?agcttaacaa?ctccgacctc?ccacaagtca?tctctacctc?ctacggtgaa 1080

gacgaacaga?ctatccccgt?cccctacgcc?cgcaccgtct?gcaacctcta?cgcccaactc 1140

ggcagccgcg?gcgtctctgt?aatcttctcc?agcggcgact?ccggcgtcgg?cgccgcctgc 1200

ctcaccaacg?acggcaccaa?ccgcacgcac?ttccctcctc?aattccccgc?ctcctgcccc 1260

tgggtaacct?ccgtcggcgc?aacctccaag?acctcccccg?agcaagccgt?ctccttctcc 1320

tccggcggct?tctccgacct?ctggccccgc?ccctcctacc?aacacgccgc?cgtgcaaacc 1380

tacctcacca?agcacctggg?caacaagttc?tcggggcttt?tcaacgcctc?cggccgcgcc 1440

ttccccgacg?tctccgcgca?gggcgtcaac?tacgctgttt?acgacaaggg?catgcttggc 1500

cagttcgacg?ggacgagttg?ctccgcgccg?acgttcagtg?gcgtcatcgc?gttgttgaac 1560

gatgcgagac?tgagggccgg?gttgcctgtg?atggggttct?tgaatccgtt?cctgtatggt 1620

gtcggaagtg?agaagggtgc?gttgaatgat?attgtgaacg?gcgggagtgt?gggttgtgat 1680

gggaggaatc?ggttcggggg?cacgcctaat?ggtagtcctg?ttgtgccgtt?tgctagttgg 1740

aatgccacga?ccgggtggga?tcctgtgtcg?gggttgggaa?cgccggattt?tgcgaagttg 1800

aaaggggtgg?cgttgggtga?ggagggtggt?aattaa 1836

<210>84

<211>1437

<212>DNA

＜213〉aspergillus niger

<400>84

atgtggctct?ttctcgtgtg?cagtatcctg?ctgccacttg?gagtagtcaa?cgcacagtct 60

caatacttca?acaacaaaac?caaagaattc?gtcgtcaatg?gctctgctat?tccttttgtc 120

gatttcgaca?ttggcgagtc?ctatgcgggc?tacctaccca?acacgccttc?tggaatctcg 180

agtctatact?tctggttctt?tccatcttct?gatcctgatg?cgtctgatga?gatcaccgtc 240

tggctgaatg?gcggcccagg?atgcagctct?ctggcaggca?tcatgctcga?gaacggcccc 300

tttctatggc?aacctggtac?ctaccgaccc?gtgcgcaacc?cttatgcctg?gaacaacctc 360

acaaatatgg?tgtacattga?tcagcctgct?ggaacgggat?tctcgcttgg?cccgtctacg 420

gtggtctcag?aatttgatgt?agccagacag?tttatggact?tctggaggcg?gttcatgaaa 480

acattcgatc?tgcagaatcg?aaagatatat?ctcactggcg?agagctatgc?gggccagtac 540

atcccataca?tcgcgtcgca?gatgcttgac?caggatgatg?atgagtattt?ccgggttgcc 600

ggcatccaga?tcaatgatcc?ctacatcaat?gagctgccag?ttttgcaaga?tgttgcgacc 660

gtcaatcagc?accgctccct?ctttcccttt?aatgacacct?tcatgagtca?aatcaccaag 720

ctttccgacg?attgtggcta?cacttcgttt?cttgacgatg?cccttacctt?tccaccccgt 780

tctcaattcc?catcagtgcc?ctataatgct?agctgcaaca?tctgggatat?cataaacaac 840

gcttctctag?ctctcaaccc?atgcttcaac?cgctaccata?tccccgacgc?ctgccccacc 900

ccctggaacc?cagtcggcgg?ccccatcgtt?ggacttggtc?cgaccaacta?cttcaaccgc 960

agtgacgtcc?agaaagccat?caacgcgtac?ccaacggact?atttcgtctg?caaggatgga 1020

atcttcccga?cggccaacgg?actggacaca?tcccctccaa?gctccctggg?accgctgccg 1080

cgcgtcatcg?aacagaccaa?caataccatc?attgcgcacg?gcctgatgga?tttcgagctg 1140

ctggcgcagg?gaaccctgat?cagtatccag?aatatgacct?ggaatgggaa?gcaggggttc 1200

gagcgggagc?cggtggagcc?gttgttcgtg?ccgtatggtg?gatcatcggg?aggaggcgtg 1260

ctgggaacgg?cacatacaga?gcgtggattg?acattttcga?cagtatttag?ttcaggacat 1320

gaaatcccgg?aatatgcacc?gggggcggca?tatcgccagc?tggagttttt?gctggggagg 1380

gttgcgaatc?tgtcgacaat?tattgagcag?gtgcagataa?cagagcagaa?tggttga 1437

<210>85

<211>633

<212>DNA

＜213〉aspergillus niger

<400>85

atgtccaaac?tctccgctgc?tatctccaag?ctctccctct?ccaccatagc?caccactctg 60

ctcctcctta?cccccccaac?caccgcctac?ttctacaaat?atcccgccct?cttcgtctac 120

aaagacacca?actgcaccga?tatctccttc?tcacttgtct?acccctccct?gggtaactgc 180

aacggcggat?actacgacta?cgcgggctca?ttccagatgt?tcaatatcga?tgctgcgtat 240

acctgtaatg?gcagtgactc?gacactgatg?tttgagatgt?ataatagctc?cggctcggat 300

tgtggagatg?agagtgattt?gttgtttaga?cagccggtga?cggaggagtg?tactgttgcg 360

gatgtggaga?gtccggggcc?gttggagatg?ccggtttggt?ttgagttggg?gtcactattg 420

gggaattgtg?gtgggatggc?tggtactatg?ttgttcggtg?tggggattct?tgagggtggg 480

ttagagacta?aattatactg?gaaatgttat?tcatcaaggc?tgaatacaag?tgtaaccgtg 540

cacagattat?ctttgatact?gtctatgggc?tgtacgagcg?tctctgactc?ctacaatgag 600

ttagcggctg?cacattacta?tgaggacctg?tga 633

<210>86

<211>1827

<212>DNA

＜213〉aspergillus niger

<400>86

atgcgtcacc?tcttatcact?gctggtgctt?ctgatcgcat?cggccgccct?ggtctccgcc 60

gtccccgccg?gctccattat?cactccacaa?ccacccgtcg?agcccgttca?ccttctctct 120

tcccagccct?ctgatccccg?aaggccatgg?atccgcctcc?gtgactggat?catcgagtcc 180

atatggggca?tcgaaaaacc?cgcatctcgt?cgattcccac?tcaacgattc?cccgcgcaat 240

cgctctcctc?cctcccggat?tctggcgcgc?tacggtagtg?acgtcgtact?tcgtttcagc 300

ctgcgcaatc?acgatgaggc?cgaggcattg?gcccaggctg?cagacattct?attcctggac 360

gtatgggcgt?ctactccagc?attcgtagat?atccgactgg?ccgaggaagt?caccgcatat 420

actcccctaa?tagacaacct?ggcagagaga?atctatacga?cctatccatc?taaaaagccg 480

ataggacttg?aaggacaatc?tggatttgcg?tcctcgagtc?gacctgcgcc?aaagttcggt 540

gacctttttt?tccacgagta?tcagcctttg?tccgtcatta?tcccctggat?gcggctgctg 600

gcttccatgt?ttccatccca?tgtgcgcatg?attagcgttg?gagtatctta?cgagggtcgc 660

gaaattcccg?ccctccgact?gagcgcaggc?agctccaccg?cggcgtcagg?ccctcgtaaa 720

acaatcatcg?ttacgggtgg?tagccatgcc?cgcgaatgga?ttggcacctc?aaccgtgaac 780

catgtaatgt?acacgctcat?taccaagtat?ggcaaatcca?aggccgttac?ccgccttcta 840

caggacttcg?actggatcat?gatccccacg?atcaatcccg?acggctatgt?ttatacctgg 900

gagacggacc?gactatggcg?caagaatcga?cagcggacca?gcctacgctt?ctgtcccgga 960

atcgatcttg?accgcgcctg?gggcttcgaa?tgggacggcg?gtcggacccg?cgctaaccct 1020

tgttcagaaa?actatgctgg?agacgagccc?ttcgagggaa?tggaagcaca?acaattagca 1080

cagtgggcgc?tcaacgagac?acaaaacaac?aatgccgaca?tcgtgagctt?ccttgacctt 1140

cactcttact?ctcaaacaat?tctctacccc?ttctcctact?cctgctcctc?gatccctcca 1200

acgctcgaga?gcctggaaga?gctaggcctt?ggcctagcca?aggccattcg?gtacgcgact 1260

cacgaaatct?acgatgtcac?ttctgcctgc?gaaggcatcg?tcacggccag?tgcggcagat 1320

aacaaccccg?ggcggttctt?ccccattggt?ggcaactccg?gtggcagtgc?gttggactgg 1380

ttttaccacc?aagtgcacgc?gacttattca?taccagatca?agcttcgtga?tcgcggaagc 1440

tacgggttcc?tccttccgtc?tgaacacatc?atccccaccg?gcaaggagat?ctacaatgtt 1500

gttctgaaat?tgggatcctt?cctcatcgga?ggcgactcat?ttgacgtcga?ttgggaatca 1560

gaactcttcg?atctgtcaaa?ggacgaatcc?gatctggata?gccgctattc?aaaatccaat 1620

gaccgctccc?cggcgtatct?acacaacgcc?aacggccccc?tgcccaacat?tgacgaagac 1680

gaagataagg?aatgggtaat?ggtggaggaa?gaagactaca?cagacgatga?cgacgacgat 1740

gatgatgatg?atgaagaaga?ggaagaggaa?gaggaagata?catattgggc?caccgaacac 1800

acatacgaat?ttcggcgacg?acgctga 1827

<210>87

<211>1251

<212>DNA

＜213〉aspergillus niger

<400>87

atggctttcc?tcaaacgcat?tctcccgctg?ctggccctca?tcttacctgc?agttttcagt 60

gccacagaac?aggtccctca?tccgaccatc?cagaccatcc?cggggaagta?cattgttact 120

ttcaagtccg?gcattgacaa?tgcgaagatt?gagtctcatg?ccgcatgggt?aacggagctc 180

cacaggcgca?gcttagaagg?ccgcagtaca?accgaagatg?accttcccgc?cgggatcgaa 240

agaacgtaca?gaattgccaa?ttttgctggg?tacgcggggt?ctttcgatga?gaaaactatc 300

gaggagatcc?gcaaacatga?ccatgtagct?tatgtggaac?aagatcaggt?ctggtatctc 360

gatacgctag?ttaccgaaag?gcgagctcct?tggggactgg?ggagcatttc?tcaccgtggt 420

gggtctagca?ccgactacat?ctatgatgac?agcgctgggg?agggtacata?cgcttatgta 480

gtggacaccg?gcatcttggc?tacgcataat?gagtttggtg?gtcgtgctag?cctggcatat 540

aatgctgcag?ggggtgagca?cgttgatgat?gttggacatg?gtacacatgt?agcagggacc 600

atcgggggca?aaacatacgg?ggtttcgaaa?aacgctcacc?tactgtccgt?gaaggtgttt 660

gtaggtgaat?ccagctcgac?atcggtcatt?ctggatggct?tcaattgggc?cgccaatgat 720

attgtgagca?agaaccggac?cagtaaggcg?gcgataaata?tgagtcttgg?tggaggctac 780

tcctatgcgt?ttaacaatgc?agttgagaat?gcttttgacg?agggtgtgct?ctcttgtgtt 840

gccgctggaa?atgagaatag?agatgcagca?cggactagcc?cggcttctgc?acccgacgcc 900

attactgttg?ccgctatcaa?cagaagcaat?gcccgtgcgt?cattctcaaa?ctacggctct 960

gtggttgaca?tttttgcccc?gggagagcaa?gtactttctg?catggaccgg?ctcgaactcg 1020

gccaccaaca?cgatctccgg?cacgtccatg?gctacacccc?atgtgacagg?tttgatcctc 1080

tatttgatgg?gcttgcggga?ccttgctacc?ccagcggctg?caacgaccga?gctcaagagg 1140

ttggctacgc?ggaatgctgt?caccaatgtg?gcgggtagcc?ccaatcttct?ggcctacaat 1200

ggaaacagcg?gcgtgtcaaa?agggggtagc?gatgatggag?atgaggacta?g 1251

<210>88

<211>1368

<212>DNA

＜213〉aspergillus niger

<400>88

atgatcaccc?ttttgtcggc?cctgttcggc?agcgtagtat?atgccgctac?gcagaccgtg 60

ttagggccag?agggggctga?tccctttacg?gtgtttcgca?gcccacactc?accggcattt 120

tcaattcgca?tccaggagca?gaatgactcg?atctgtgatg?ctcgttcacc?ccaattcact 180

ggttggctcg?acattggccc?gaagcatctt?ttcttttggt?attttgaaag?ccagaatgac 240

cccttccatg?atcccctaac?gctatggatg?actgggggcc?caggagactc?gagtatgatt 300

ggacttttcg?aagaagttgg?cccttgccgg?attaatgagt?ttgggaatgg?aacagatcac 360

aacccctggg?cctggaccaa?gaattcatca?cttctttttg?ttgaccagcc?agtcgatgtc 420

gggttttcct?atatcgatga?gggctatgag?ctgcctcatg?actcacgtga?agccgcggtg 480

gacatgcatc?ggttcttgcg?attattcata?tccgagattt?ttcctcacaa?acagttcctt 540

cccgttcacc?tttccggtga?atcttacgca?ggccggtaca?ttccttatct?ggcgacccaa 600

atcttggaac?aaaatgaact?gtataaagat?agccccagga?taccgctgaa?atcgtgcttg 660

gtgggtaacg?gattcatgtc?acccaaggat?gcaacgttcg?ggtattggga?aacactgtgt 720

actactaact?caggagtccc?atctcctatc?ttcaatgaaa?ctaggtgcga?tattatggcg 780

gcgaatatgc?cgcactgtat?ggatctatat?gacatatgca?ttcaacactc?agaccccgcg 840

atatgtcatg?cggcccagtc?cgtctgttac?gatagtgttg?tagggctcat?ggccaaatta 900

ttgctaagga?tgacgacagt?cactgcacct?tgtgagatcg?acgaaatgtg?ctatatcgaa 960

gcggctctaa?ttgagagata?tttgaattcg?ccatctgttt?gggaggccct?gtcgccaccg 1020

caacaggtta?ccgaatacaa?attcgtcgct?acttctgtta?ttgatgcatt?tgctcaatca 1080

gcggacggca?tggtgtcgag?ctcgaagcag?atcgctttct?tactcgcaaa?taatgttgac 1140

ttcttagcgt?atcaaggcaa?ccttgatctc?gcctgtaata?cggctggcaa?cctacgttgg 1200

gcgaactcgc?tttcttggaa?aggccagaca?gaatttaccg?caaagccctt?acttccgtgg 1260

gaaattcagg?tttcggtcgg?tgaagggacg?gacgaaacgt?cacgctttgc?ctttgtgact 1320

gtggacaacg?ctggacacct?gttgcgggac?tcaaagattt?caaactga 1368

<210>89

<211>2376

<212>DNA

＜213〉aspergillus niger

<400>89

atgcggtttc?tcacttattc?cctgcccttc?attgcaagtg?ctatctcgct?ctttggggtc 60

aatgtacaag?ctcgatcaca?agctccaagt?gccatccgtc?atgtgtcgac?gcttgaccag 120

cccaccatca?agacaccctc?acagcgggtc?gatcaccttg?accactttga?catcaccttc 180

aatattcatg?acaagcacca?gcggataaag?ctggagctgg?agcccaacca?tgacatcctg 240

gcggaagacg?catccgtaca?gtatctcgac?gcggacggga?acgtgcgacg?gcacgagccc 300

attgctccac?atgagcataa?ggtcttcaag?gggaggagtc?tactcgggcg?aggaaaaggc 360

atgtgggatc?cggtcggatg?ggcgcggatc?tacttgaagc?aggatggctc?agagccacta 420

tttgagggag?tcttcagtat?cgacggcgac?aaccatcacg?ttcagctgaa?atcggcatac 480

atggagaaga?aacgccccgt?ggatgtcgac?cttcccgact?cagcgactga?ctatatgatc 540

ttctaccggg?attcggatat?ggtgcgtcta?catacggaac?tcaagcggtc?gtcgctcgga 600

tcgacctcgt?gtcaagccga?tcagctcggc?ttcaacacta?accccaacca?ccctgtgcta 660

caaccgtatg?gccaggcaga?gaccgatacg?tggggagcaa?tttcattgaa?ctccttgttt 720

ggactcaaca?agcgccaatc?cgatatcgga?agtgtgtctg?gcaatgcggg?cggagtcaat 780

ctggcgtcga?ccattggtga?tacttcgggc?tgtccgagta?cgaagcaagt?agctttgatt 840

ggtgttgcaa?cggactgcgc?ctttaccggc?tcattcaaca?acgagactgc?cgccaaggaa 900

tgggtcatca?gtactgtcaa?cagcgcgtcc?aatgtctacg?aaaagtcctt?caacattacg 960

attgggctgc?ggaatctgac?tatcaccgac?agctcatgcc?ccgacaaccc?gcccgcggcc 1020

acggcatgga?acatgccctg?ctccagcggc?aatctcacct?cccgactgga?tctgttttcc 1080

aagtggcgcg?gtgagcaatc?ggatgacaat?gcttattgga?ccctgatgag?cgattgcgcg 1140

acgggcaacg?aggtcggact?gtcatggctt?ggccaactct?gcaatagcga?tgcttcttcg 1200

gatggctcga?gcacggtcag?tggaactaac?gtcgtcgttc?ggtcttccgg?ctcggattgg 1260

cagatctttg?ctcatgaatc?tggccacacc?tttggcgctg?tccacgactg?tgactcccag 1320

acctgcgcgg?aggatctcga?agcctcgtcc?cagtgctgtc?cgttgacctc?gagcacctgc 1380

aacgccaacg?ggaaatacat?catgaatcct?acaactggaa?cagacatcac?tgcgttctcg 1440

caatgcacta?tcggaaatat?atgcgcagcc?ctgggccgca?acagcgttaa?gtccagttgt 1500

ctctccgcca?accgcgacgt?caccacctac?actggcagcc?agtgcggcaa?cggaattgtc 1560

gagtccggcg?aagactgcga?ttgtggcggg?gaagatggtt?gcggcgacaa?caactgctgc 1620

gacgcgaaga?catgcaagtt?caagtcggga?gctgtgtgtg?atgactccaa?cgacagctgc 1680

tgttcaagct?gccaattctc?ctcagctggg?acggtatgtc?gtgccagtcg?cggcgactgc 1740

gacgtggcag?agacctgcag?cggcaactcc?agtacttgtc?ctaccgactc?gttcaagaag 1800

gacggcacga?gctgcggcag?cagtggctcg?ggacttgcct?gcgctagtgg?ccaatgcacc 1860

agccgcgact?accagtgccg?cagtgtgatg?ggcagtctcc?tccacagcaa?cgacacctac 1920

gcctgttcct?ccttcagttc?ctcctgcgaa?ctggtctgca?cctccccgaa?gatcggcacg 1980

tgctacagcg?tcaaccaaaa?cttcctcgac?ggcactccct?gcggtagtgg?cggctactgc 2040

agcaacggcg?actgcaaggg?ccaaaacgtc?gaatcctgga?tcaagaacca?caaaggtatc 2100

gtcattggtg?tcgcctgcgc?cgtaggcgcc?ctgatccttt?tggccctgat?gacctgcatc 2160

gtaaaccgct?gtcgccgggc?tcgcgcgcca?aaacccgtcc?cgcgtccagt?gccttacggg 2220

ccgtggcccg?gcgctaggcc?tcccccgccg?ccgcccatga?accagtggcc?ggcgcgaggc 2280

tatcaaggct?tagggaatga?gccgccgccc?ccgtatccag?gtgtacctgg?tcagccagta 2340

ccgcaacata?tgcctcccca?ggggcggtac?gcttga 2376

<210>90

<211>1446

<212>DNA

＜213〉aspergillus niger

<400>90

atgcgtttcc?taagcagtgc?agccctattc?ggcctggcgt?atgcctccac?ccaggcggtc 60

ctccagccag?aggaaccatc?cgacttccgt?acattccaca?gcccatattc?cccgcaccac 120

tcgatccgca?tccgccagca?gaatgaatca?atctgcgctg?cccattccgc?ccaatacacc 180

ggctggctcg?acatcggccg?taaacatctc?ttcttctggt?actttgagag?ccagaatgac 240

cctgccaatg?atcccctcac?tctctggatg?acaggagggc?caggggggtc?cagcatgatc 300

ggtctgtttg?aagaagtcgg?gccatgtctg?atcaatgagt?acggcaatgg?cacttactac 360

aatccgtggg?gctggtcccg?gaactcctcc?ctactatttg?tcgatcagcc?agtcgatgtg 420

ggattttcgt?acgtcgatga?aggagaggac?ctgccgggcg?attcgcatca?agctgcaatt 480

gacatgcatc?ggttcttgca?gttgtttgtc?tcggaggttt?tcccgcaatt?gcagactctt 540

cccgttcatc?tttctggtga?atcgtatgct?ggtcactatg?tcccttacct?cggcagtcag 600

atcgtccaac?agaacaagct?ctatcccact?gagccccagg?tccttctgca?ctcatgtctc 660

gtaggcaacg?gctactattc?tcctcgcgac?actacctacg?gctactggga?aaccctctgc 720

accactaacc?ctggagtccc?cgagcccgtc?ttcaaccgaa?ccagatgcga?catcatggcg 780

gccaatatgc?cgcgatgcat?ggaagtatcc?gacgtatgtg?ttcggaaccc?cgatccagct 840

atctgccatg?ctgcgtcgga?ggtatgctac?gagggcgtga?tcggatggta?tgatgacgag 900

tctggtgaag?gtggtcggaa?taggtttgat?ataaccgctc?cctgcgccct?tgacggcata 960

tgctacatcg?aggccgctcg?catcgagcag?tacctgaaca?cacccgcagt?ttgggctgct 1020

ctatcaccac?ccaaagaaat?caaagaatac?aaggttactt?ccgacaatgt?gtcgcgcgca 1080

ttcgatctca?cttcagacac?gatgacgcca?gcgtctgagc?aagtcgcgtt?cctgcttgcg 1140

aatcaggtac?atttcctggc?gtatcagggc?aatctcgatc?tggcgtgtaa?tacggcgggt 1200

aatctgcgct?gggcgcattc?tctgccatgg?agaggtcagg?tcgagttcgc?gtcgaaggcg 1260

ctgcggccat?ggagttgggt?agatgtggta?tctggaaaag?gtggagtggc?tggaacgacg 1320

aaggaggagt?cgaggtttgc?gctagttacg?gttgatgggg?cgggacattt?tcttcctcaa 1380

gatagacctg?atatcgcgtt?ggatatgatg?gtgcgctgga?tatccggggc?atcgtttact 1440

gagtga 1446

<210>91

<211>960

<212>DNA

＜213〉aspergillus niger

<400>91

atgacattgt?tactcaactt?ccacgcgctc?tttacagtca?ttcttgttgc?caatctttca 60

accagatgca?gcgcactgct?ctctggacgt?gacttttgct?ccacgccagc?gcccggtgag 120

tcactccgag?cggagcatag?gaggctgtat?gatgtacagg?cccaacgtga?cagcaccgcc 180

gaggagagcc?gggaggtggt?gccttggatt?gaaatcgaga?catggtttca?tattgtaagc 240

agcaatgaag?cagcaaacac?agtatcagac?gacatgatca?ccagccagct?ttcctatctt 300

cagaaggcat?atgaaagtgc?gactatcacc?tatcggttgg?agggcataac?tcgtcacata 360

aatgactcgt?gggcgcgaaa?tgatgatgaa?ctggggatga?agaatgccct?acgaaggggc 420

aattatggca?cattaaatgt?ctatttccaa?acagatctcc?aggcgtcatc?cgacgagaat 480

tctcgggact?atccaaatga?cggtaaccga?cgaacagatg?tgtcagatca?atcatcatca 540

actgtcctag?gcttctgtac?gttgcctgac?ccgagtgtga?attccagcag?ccctcgttcc 600

agctacatca?aggatggttg?taacgtgtta?gcggatatca?tgccgggtgg?tagtttagcg 660

cagtacaaca?aaggcggcac?agcggttcat?gaggttggcc?attggaatgg?gctgctgcat 720

acgttcgaag?gtgaatcgtg?ctcccctgat?aatgaaggag?attacattga?tgacaccccg 780

gagcaatctg?agcctacgag?cggatgtccc?gccgagaaag?attcatgccc?cgatcttcct 840

ggccttgatg?ctattcataa?ttttatggac?tattcatctg?atgactgtta?tgagagtttt 900

actccagatc?aagcggagag?aatgaggagt?atgtggtccg?ctatgcggga?agggaagtga 960

<210>92

<211>1920

<212>DNA

＜213〉aspergillus niger

<400>92

atgcatgtct?cacttttcct?actcagtgtt?acggcagcgt?ttgccagccc?aacaccccat 60

aactatgttg?ttcatgagcg?gcgcgatgca?ttgcccagtg?tctgggtaga?agaaagccgg 120

ctggacaaag?gtgccctact?gcctatgcgg?atagggctta?ctcagtccaa?cctggatcgt 180

ggccatgatt?tattgatgga?ggtgtctcat?ccacaatcgt?ctcgctacgg?aaagcatctc 240

tccagcgagg?aggtgcacga?cctatttgcc?ccgtcgaatg?aggccgtcga?gaccgtccga 300

acctggattg?aatccgccgg?aattgctcca?agccgcatct?cgcaatcata?caacaagcag 360

tggctacagt?tcgatgccca?tgcaagcgag?gttgagcagc?ttctgcagac?ggaatactac 420

atctacaccc?atgccgacac?gggaagttcc?catgtgacat?gccacgagta?ccatgtgccc 480

gaaaccatcc?aatcgcacat?cgactacata?acaccaggag?taaagatgct?ggaagtgcgc 540

ggcacgccct?ccaaaaagag?agatgcagag?aagcgctctc?ttggcagtct?gcccccaatc 600

ttagcaccac?taccaatcaa?tatcacgaag?attttcgacg?acccgctagc?acactgcgat 660

ctggcggtaa?ccccagactg?cattcgagcc?atgtacaaca?tcaccaaagg?aacaacagcc 720

acaaagggca?acgagctcgg?catcttcgag?gacctaggag?acatctacag?ccaagatgac 780

ctcaaccttt?tcttcgccaa?ctttgccagc?gacatcccac?agggaaccca?tccaaccctc 840

gactccatcg?acggcgccac?cgccccaaca?gacgtcacca?acgccggccc?cgaatccgac 900

ctggacttcc?aaatcgccta?cccaatcatc?tggccccaga?acaccatcct?ctaccaaacc 960

gacgacccca?actacgaaga?caactacaac?ttcaaaggac?tcctcaacaa?cttcctctac 1020

gccatcgacg?gctcctattg?caacgaaacc?tcctctctag?accctcaata?cccagatccc 1080

tccccaggcg?gctactcctc?ccccaagcaa?tgcggcgtct?acacccccac?aaacgtaatc 1140

tccatctcct?acggcagccc?cgaagccgac?ctccccatcg?cctaccaacg?ccgccaatgc 1200

cacgagttca?tgaaactcgg?ccttcagggc?atcagcgtgg?tcgtcgcatc?gggcgactcc 1260

ggcgtcgcct?ccagcacggg?cacctgcttt?ggcgatgcag?acaacgtctt?cgtcccagat 1320

ttcccagcca?catgtcccta?tctcaccgca?gtaggaggca?catacctccc?cctaggcgca 1380

gacgcagcca?aggaccagga?aatagcagtc?acccgcttcc?cctccggcgg?cggcttcagc 1440

aatatctacg?cccgaccatc?ctaccagaac?cactccgtgg?agacctattt?ctccactacc 1500

agcgacgacc?tcacctaccc?ttactactcc?ggagtaaact?acacagactt?ctccaacaca 1560

gatggggtat?acaaccgcat?cggacgagga?taccccgatg?tttcagctat?cgcagacaat 1620

atcatcatct?acaaccaggg?cgaagcgaca?ctggtgggtg?gtacgtctgc?cgcggcgccg 1680

gcgttcgcgg?ccatgttgac?gcgcattaac?gaggagaggc?tggcgaaggg?gaagtccacg 1740

gtggggtttg?tgaacccggt?gctgtatgaa?catcctgagg?cgtttaggga?tgtgactgtt 1800

gggtcgaatc?ccgggtgtgg?gactgatggg?ttcccggttg?ctggggggtg?ggatccggtg 1860

acggggttgg?ggacgccgcg?gtttgaggat?ttgatggata?tatttgtggg?tgatgattga 1920

<210>93

<211>1116

<212>DNA

＜213〉aspergillus niger

<400>93

atggcttcca?agaccctcct?actcattccg?gcactggcca?cagccgctct?gggaagtgta 60

ttggacctag?atatcaaggt?agaccttgga?accccaggtg?gcccgtttga?tttgatgtac 120

gacaccggat?catcaacact?ctgggtgctt?gatagcaatt?gtacagatga?ttgtccaaat 180

gttagcgggt?actcccgaca?cggctacaac?ctcacctcta?ctggtgtcaa?cttaggtgtc 240

aacgacagca?ttgcttacag?cggaggcact?gtcagcggct?tcactgccac?ggatattctc 300

acggttcccg?acaccaacgt?ctcatatcgc?cagagctttg?ccgtcattac?cgacagtacc 360

tgggcggcct?tagcagccga?tgggttcatc?ggcctggcat?cgtctaccat?cgcattcaag 420

aatactacga?cagccgtcga?acagatgatg?caggatggac?ttttggatga?acctcgattc 480

gccatatatg?caggttcagg?ggaatcgacc?gtgaccaacc?ctaatccgga?gaataatggc 540

gtgttcacct?ttggtggcag?ccatgaggaa?acctatgcgg?acggggaact?gcaatggatg 600

aagatgctct?ccccctttga?aatatacaaa?acaaatctcc?ttggaattca?gggacacaac 660

aactccgatg?gccaggccct?gtcaagcgac?gtcctgaact?ggtacggcca?gactaatcta 720

ttcaacgtcg?caggtgcttc?atcgataagc?attcccaacg?accagattga?ggcgatgtat 780

gccctaacgc?ctttctcata?cgctgacatc?tcatctggat?accgacctct?gtgctccgat 840

ttcaatgata?catggtcgat?ctcttttaca?atgggcttct?atggcgaggg?tgtcaccttc 900

aatttgaccg?gtgatcagct?ggccgtgcct?ggctatcagg?acgacgacca?ctgcttccct 960

cccttcaatc?catgggacag?ctacaacacg?attattggtc?agcattggtt?gagcaatttc 1020

tatgctgtat?tcgacttcgg?atcattcgac?ccggagacat?acgatatacg?tgttgggctg 1080

gctcctttga?agaaggaata?cctgccgagc?gcttga 1116

<210>94

<211>1245

<212>DNA

＜213〉aspergillus niger

<400>94

atgtttccct?gctctcgtat?ttggtctctg?ctcgttgcag?ccgccaccgc?tagtgctgta 60

cccaccagtc?tggccaccac?gcacctgcaa?tcggttgact?tgcttctgac?tcgcagttct 120

tacgggtttc?ttactgacat?agcccttgga?actccgggtc?agagcctgcc?gtatctggtt 180

gactggacct?ggaccggcca?ctatgtggtg?accaccttgt?gctacaacga?tcccaccgcc 240

acctacgatt?gtctcaacgt?cgatcagaaa?attttcaacc?agactttgtc?atccactttt 300

atcaaccaaa?ctgaccagta?tggctatctt?tactgggatc?ccaaccactt?ctactttacg 360

gagcccgcag?cagccgatgt?ggcgacggac?atgctgcgca?tcggtcccac?cgcggtgaac 420

accaccatcc?aagcagccaa?tttcgtcttc?aacgagacta?ttagcgcatt?ccctttctcg 480

ggagtatatg?gactctcacc?tgtttttcag?ggtgacaatc?gatccgtgca?agcgtccttc 540

taccaaggat?ggaggagcgg?cgcctggcac?tctccaattg?tctcttttat?ctactgccac 600

gacaatgcca?ccaaagcggt?atgcagtggt?tacgacggcc?ttcagacact?aggcggatac 660

aacacctctc?acgtccaggg?agatatcacc?tggtacgaca?tcattgtcac?ggaggcgatc 720

aacacgctgg?actttgtcta?tgcgccagcc?gtgattaatt?attgggcgtt?gaacctcacg 780

cgcttctcta?tcggagacga?agagcaagag?ctcaacaaga?ccactactct?ggatggaaag 840

caagccgccg?ttgccgcgtt?cgaccacgct?tcgtatggtc?gcggtgcccc?agtgtctgtg 900

tacggttacc?agcgtctagt?cgagctggtc?ggggcaaaag?ccgtcacgct?ttccgatcct 960

ccaaataacg?gtgagcaggg?attctatcag?ttcgattgcc?ggaactcgag?tttactgcca 1020

ccgctgcggt?atgagtttgc?cgggtcagag?cgggcgtggg?agattgtgcc?cgagaactat 1080

gtggaggtgc?tggcgaacgg?aaccaataag?tgcaccttta?atgtacgcac?cctgggagat 1140

ggagcgatgg?taatgggaaa?ttttggcgag?acatttgcca?ttgataagta?tgtcatgttt 1200

gactttgaga?agttgcaggt?ggggattgca?gacttcgcgt?ggtaa 1245

<210>95

<211>1443

<212>DNA

＜213〉aspergillus niger

<400>95

atgcatctcc?cacagcgtct?cgttacagca?gcgtgtcttt?gcgccagtgc?cacggctttc 60

atcccataca?ccatcaaact?cgatacgtcg?gacgacatct?cagcccgtga?ttcattagct 120

cgtcgtttcc?tgccagtacc?aaaaccaagc?gatgctctag?cagacgattc?cacctcatct 180

gccagcgatg?agtccctgtc?actgaacatc?aaaaggattc?ccgttcgtcg?tgacaatgat 240

ttcaagattg?tggtagcgga?aactccctct?tggtctaaca?ccgccgctct?cgatcaagat 300

ggtagcgaca?tttcatacat?ctctgtcgtc?aacattgggt?ctgatgagaa?atctatgtac 360

atgttgctcg?acacaggcgg?ctctgatacc?tgggttttcg?gttccaactg?cacgtccaca 420

ccctgcacga?tgcacaatac?cttcggttcg?gacgattctt?cgacccttga?aatgacatcg 480

gaagagtgga?gtgtgggcta?tggaactggg?tctgtcagcg?gcttgctagg?aaaagacaag 540

ctcacgattg?caaatgtcac?tgtacgcatg?actttcggac?ttgcttccaa?cgcatcggat 600

aacttcgagt?cgtacccaat?ggacggcatt?ctcggtctcg?gtcgaaccaa?cgatagttcc 660

tacgacaacc?caacattcat?ggatgccgtt?gcagaaagta?acgttttcaa?gtcgaatatc 720

gttggcttcg?ccctttcacg?tagccccgcc?aaggatggca?cggtcagctt?tggcactact 780

gacaaggaca?agtacaccgg?cgatatcacc?tacaccgata?ccgtcggatc?ggacagctat 840

tggcgcattc?ccgtggacga?tgtctatgtt?ggcggcactt?catgcgattt?ctccaacaaa 900

tcagccatca?tcgataccgg?aacttcttat?gctatgctgc?cttcaagcga?ctcgaagacg 960

ctgcacagtc?tcattcccgg?cgccaaatct?tcggggagct?accacattat?tccgtgcaac 1020

acaactacta?agctacaagt?ggcattctct?ggtgtgaatt?acaccatctc?gccgaaggac 1080

tacgtgggag?caacttcagg?ttctggatgc?gtttcgaaca?ttatcagcta?cgacttattt 1140

ggtgatgaca?tctggctcct?gggtgacacg?tttctcaaaa?atgtgtatgc?tgtgtttgac 1200

tacgatgagt?tacgggtcgg?atttgcagag?cgttcctcga?acaccacctc?tgcgtcgaac 1260

tctacgagct?ctggaacaag?cagcacctcg?ggatccacta?caacgggcag?ctcaacgact 1320

acgacgagct?ctgctagctc?tagtagttca?tctgatgctg?aatcaggaag?tagcatgacc 1380

attcccgctc?ctcagtattt?cttctctgct?ctggcgattg?cttccttcat?gctttggctc 1440

tag 1443

<210>96

<211>1401

<212>DNA

＜213〉aspergillus niger

<400>96

atgacgtctt?ctaccttgcg?ccttgccgtc?gcgttggctt?tgtcaacttg?cagcagtgcc 60

ctatcgagcc?agcgagatga?ttcacttgtg?gttccatttc?cttttggcaa?tcttgaggat 120

gtccatattg?ccaagcggga?tagctccaag?acagtagaag?ctcctctagt?gatatatggc 180

gacagctact?ggatgaacgc?ctcaattgga?acccctgcgc?agtcactaag?tttcctacta 240

gatcttacgc?gctcaagggt?cgagcccgca?tacaccctcg?atgagaatta?cgaatgttct 300

gacgatgaac?tctgctccga?attcggcttc?tacaaaccca?ccgattcatc?cacttatcag 360

catctcacct?acacacagag?acacgatgca?ggtgtcgact?actcctacct?tgataccata 420

actcttggag?atcacgcaac?cgacaatgtc?ccactggaca?tgtatctttt?gtcctacatt 480

tcctacagct?ccctcggtct?ctcctccgtc?aacaccagct?tcccctacat?cctggtcgat 540

cgcggcctca?ccacctcccc?atccttcagc?ctaatcggcg?acaacggaaa?caccaccacc 600

cccagcatca?tctttggagg?catcaacacc?tccaaattca?acgggcccct?gcaagccttc 660

tccttcgcag?accacagcat?caccaacaat?ccattcgtca?ccgtcgaagc?tgactccctc 720

caactaacca?ccaacaccaa?cgataattcc?acctacccta?ttccctcctc?cacccccatg 780

atgctcagaa?ccgaagaact?aatcacctac?ctccccaact?cgaccgtcca?atccctctac 840

accgacctta?acataaccat?ggacggcgtg?atctccactt?caagattcta?cggggtcctt 900

ccctgcgccc?gccaggaaac?cgaatctcac?acaatctctc?tagccatcgg?caacatgacc 960

ttctctgtgt?cctgggatga?gctcttcgtc?ccgtggacgc?gtgacggact?atgcaagttc 1020

ggcattcagg?cccaggattc?agattacaaa?actcgtgcgg?agctgggtgt?tccctttctg 1080

agacggatgt?atgtcgctgt?ggattataat?aatcagtttg?tgggcgttgc?gacgctgaag 1140

gatgatgatg?atcagaatgg?aggtgaagat?gagattgtgg?agattggcac?tgggacggcg 1200

ttgcctagtg?ctgtcgggga?ttggccggct?agtgttacgg?cgtatacgcc?tgctgcttct 1260

acagggacgg?cggctgcgac?gttgacattc?acgacggcga?cgtctagcgg?gggaggtgtg 1320

gtgccgacgg?gtctatcaga?gttgggtagg?gcgtttttgg?tgccgggggt?gctggggatg 1380

gctgttttgc?aggctgttta?g 1401

<210>97

<211>1632

<212>DNA

＜213〉aspergillus niger

<400>97

atgatgcgac?cgatacttct?ccccctactg?ggggtatttc?tgcagacctc?ctcggcatcc 60

aatccctatg?taatgagctg?gtcttcccaa?gcctacggtc?cagatggccc?gtggcaggcc 120

gtatccatcg?acgtgggcag?caaccagcag?acggtcgatc?tttaccccgg?agccaactat 180

gctagcacga?tcctgatgag?cactctctgc?acgaacaaaa?ccctgtcatc?cacctgctac 240

gctgccgaag?caggcacgtt?caaccaaaac?acctccacca?ctgcctacac?caccgccagc 300

tcgtgggaaa?caacttactg?ggccgtcgag?ggtggaagcc?aagaggctgt?gctcggcgat 360

gaggtcacct?tagggtcgtt?tgtcgtcccc?aatgtgagct?tcgaagccat?ctaccagacc 420

taccagacct?atcccaatgg?catcgcctat?cctgtctcgg?tcggcagtct?ggccctgggg 480

ggtccgtact?tgtcggatac?cgtctccaat?tcgacggtcc?tgaacatgat?cgcaggatgg 540

ctttactcgt?ccaacgacat?tccgtcctac?tcgtacggca?tgcatatcgg?gtcggtagac 600

cccaaaatcc?caggctccct?gatcttgggg?ggctacgata?agagccgagt?gatcggagac 660

gtgagtgcgc?agggagtagt?gtcttcgagt?ggtcttttgg?aacttgaatt?aaaggatatt 720

gggctgggtg?ttgcggcggg?ttcctctccc?ttcagcttca?acaacgaaag?tggcttgttt 780

ctccaaagca?gtggttcggt?tcaggccaag?accgtccaga?ttgatccaac?caagccctac 840

atgtaccttc?cccaggcgac?atgcgatgcc?atcacctcca?ccatgccgat?ctccttcaat 900

tccagcttgg?ggctatactt?ctgggacacc?acgagcgatg?attatctgaa?tatcacgtct 960

tccgccgcat?acctctcctt?tgtgttcaac?atgaatgggg?tcaacaacaa?gaacattacc 1020

atcaagattc?ccttttccca?gctcaatctt?acgctgcaag?aaccgctggt?cgatcaaaac 1080

gtcacctact?tcccgtgctt?cctcactacc?tccaccccgg?tgctcggtcg?agcctttctc 1140

cagtccgcat?tcgttggggt?gaactggttc?aacgggaaca?actcgggcac?atggtttctg 1200

gcacaggccc?ccggcccggg?ttacgccagt?gaagacatca?cccggatcgc?agtgagtgac 1260

acgtcgcttt?ctgcctctaa?cggtacctgg?gaagagacct?gggctacgta?ctggggcatc 1320

aaaacatccg?acaactcgag?cagctccaag?agtggcctgt?cttccggtgc?caaaattgga 1380

attggcgtcg?gggtgggtgt?cggtggagca?gtgttgatcg?cagcaggtat?agccattgca 1440

ttctgtcttc?gccgtcgccg?cggggcgagt?caagaggcgg?ctggagagca?acggaggtcg 1500

atgtttaggg?gctttgcgga?gctaccggga?ggtgctcaca?gtgaaccggc?gaaggagttg 1560

gatacgaaga?tgcataagcc?gccgcaggaa?atgatggctt?cgcaggaggt?agagcgatac 1620

gagctggggt?ga 1632

<210>98

<211>2535

<212>DNA

＜213〉aspergillus niger

<400>98

atgcgtctta?caggtggtgt?cgctgcggct?ctgggcctct?gcgctgctgc?ctccgcttct 60

ctccatcccc?atcgttccta?cgagacccat?gattacttcg?ctctacacct?tgatgaatcc 120

acctcgccgg?ccgacgtcgc?ccaacgacta?ggtgctcgcc?acgaaggccc?cgtcggagaa 180

ttaccctcac?atcatacctt?ctcgataccc?cgtgaaaaca?gtgacgatgt?ccatgcgctg 240

ctggatcaat?tgcgcgatcg?tcggaggtta?cgccgccgct?ccggagatga?cgccgctgtc 300

cttccctcct?tggtcgggcg?agacgaaggt?ctaggtggca?ttctttggtc?cgagaagctg 360

gctccccaga?gaaagctcca?taaaagagtg?ccgccgacag?gatatgctgc?cagatcgccc 420

gtcaacactc?agaatgaccc?ccaagcgctt?gcggcgcaga?aacgcattgc?ctcggaattg 480

ggcatcgcgg?accccatctt?cggcgaacaa?tggcatttgt?ataatactgt?tcagttgggc 540

catgatctta?acgtgacggg?tatctggctg?gagggcgtta?cagggcaggg?tgtcacgacg 600

gctattgtcg?atgacggttt?ggacatgtac?agcaacgatc?ttaggccgaa?ctattttgcg 660

gcgggttctt?atgactataa?cgacaaagta?ccagagccga?ggccgcgctt?gagcgatgac 720

cgccacggta?ctagatgcgc?gggtgaaatc?ggtgcggcga?agaacgacgt?gtgcggggtt 780

ggtgttgcgt?atgatagtcg?catcgctggt?attcggattc?tctccgcacc?cattgatgac 840

actgatgagg?ctgcggctat?taactacgcc?tatcaggaga?acgatatcta?ctcgtgttcc 900

tggggtccct?atgacgatgg?cgccacaatg?gaagccccgg?gcaccctgat?caagcgggcc 960

atggtcaatg?gtatccaaaa?tggtcgtggt?ggaaaaggct?cggtttttgt?gtttgcggct 1020

ggtaacggtg?ccattcatga?cgataactgt?aactttgacg?gttacaccaa?cagtatctac 1080

agcatcacgg?tgggtgccat?tgatcgggag?ggtaaccatc?ctccgtattc?ggaatcctgc 1140

tcggcgcaac?tggtggttgc?ctacagcagc?ggcgccagtg?atgcaattca?taccacggac 1200

gtcggcacag?acaagtgctc?gactacccat?ggtggaactt?cggcggccgg?cccgctcgct 1260

gcgggaaccg?tggcgctggc?cctcagtgtg?cggccggaac?tcacctggcg?tgacgttcag 1320

tatttgatga?ttgaggcggc?agtgcctgtt?catgaagatg?atggaagctg?gcaggacact 1380

aagaacggga?agaagttcag?ccatgactgg?ggatatggta?aggtcgacac?atatacgctg 1440

gtgaaacggg?cagagacctg?ggatctggtg?aagcctcaag?cctggctcca?ttccccctgg 1500

cagcgggttg?agcatgagat?cccacagggc?gagcagggct?tggctagttc?gtacgaggtg 1560

acggaggata?tgttgaaggg?agccaacctg?gaacggctgg?agcatgtcac?ggtcaccatg 1620

aatgttaacc?acacccgccg?aggcgatctc?agcgtggagt?tacggagccc?tgacggtcgg 1680

gtcagtcacc?tcagtacgcc?ccggcggcca?gataatcaag?aggtgggcta?tgttgactgg 1740

accttcatga?gcgttgctca?ctggggcgag?tccgggattg?gcaaatggac?tgtgattgtc 1800

aaggacacca?atgtcaacga?gcatactggg?caattcatcg?attggcgact?caacttgtgg 1860

ggcgaggcga?ttgacggagc?cgagcagcct?ctccacccca?tgcctactga?acacgatgac 1920

gaccacagct?atgaggaagg?aaacgtggct?accacgagca?tcagcgccgt?tcccacgaaa 1980

accgagctgc?ctgacaagcc?cactggtggc?gttgatcgcc?cggtgaacgt?taagcctaca 2040

acatccgcga?tgccgaccgg?tagtcttaca?gagcccatcg?atgatgaaga?actccagaag 2100

acccctagta?cagaggcaag?ctcaacacca?agtccttctc?cgaccaccgc?gtcagatagt 2160

atcctgcctt?ccttcttccc?cacgttcggt?gcgtcgaagc?ggacgcaagt?ttggatctac 2220

gctgcgatcg?gctccatcat?tgtgttctgc?attggcctgg?gcgtctactt?ccatgtgcag 2280

cgccgcaaac?gtattcgcga?cgacagccgg?gatgactacg?atttcgagat?gatcgaggac 2340

gaggatgagc?tacaggcaat?gaacggacgg?tcgaaccgtt?cacgtcgccg?gggtggcgag 2400

ctgtacaatg?cttttgcggg?cgagagcgat?gaggaaccgt?tattcagtga?tgaggatgat 2460

gaaccgtatc?gggatcgggg?gatcagcggc?gaacaagaac?gggagggcgc?agatggagag 2520

cattctcgga?gatga 2535

<210>99

<211>450

<212>DNA

＜213〉aspergillus niger

<400>99

atgaagacct?tctctaccgt?cacctctctc?ctcgctctct?tctcctcggc?tctggccgca 60

cccgttgaca?gcgctgaagc?cgccggcacc?accgtctctg?tctcatacga?cactgcctac 120

gatgtctctg?gagcttcctt?gaccaccgtc?tcctgctcgg?acggtgccaa?cggcctgatc 180

aataagggct?actccaactt?cggctccctt?ccgggcttcc?ccaagattgg?aggcgcccct 240

accattgcag?gctggaactc?tcccaactgc?ggcaagtgct?acgccctgac?gtacaacggc 300

cagacagtca?acattctggc?cattgattcc?gcacctggtg?gcttcaacat?cgctctggag 360

gccatgaaca?ccctcaccaa?caaccaggcc?cagcagctgg?gtcgtatcga?agctacctat 420

actgaggtgg?atgtcagtct?ttgcgcataa 450

<210>100

<211>891

<212>DNA

＜213〉aspergillus niger

<400>100

atggctcaaa?tattctggct?ttcactcttc?ctgcttgtct?cttgggtcag?agccgagtcc 60

aaccgcaccg?aggtggacct?gattttccca?agaaatgata?cctttgcgcc?aatgcctttg 120

atgccggttg?tattcgccgt?tcaagcccct?tccgtcgccc?ataaagttaa?tacatacatc 180

gagtacggct?attacccagt?aggccgtcca?aatgaaacag?ttattggcca?gaccgaccat 240

gtgtccgact?caacaaacga?aaccacttat?ttcagtgtct?ctggtatcgg?cagaacgttc 300

aataccactg?gcagctggga?gctgttttgg?aggctgagat?ggaccaattg?ttcaatctca 360

gaagactcga?gatactacaa?ccaatcctac?ccctggatat?cctccccata?catcgacggt 420

agcctcaaca?tcgacaaggt?ctatgagggc?tttcactaca?cagcatacaa?tgtcattgtc 480

gacagggtta?ccttcagcac?tcgcgaagat?gctagccaac?ccaacctcac?gaccctcacc 540

aatagcgaga?actgcgataa?agtctcgtct?cttgctctat?tgtcgattgt?ggactcccta 600

aggattccac?cccagttacc?ccaagaagat?attgataccg?tgtcaatgtg?cccacaactc 660

gccgatgcca?ggctaaattc?aacttcaact?tcaagcccct?gcagcgttag?cattagtccc 720

gaggttgagt?ctaatatcct?ggccaagatc?gcagacaatg?aatgcaataa?cgcacttcac 780

cccgctgtga?gttgcaccac?tgaagaaacc?aaggaaggca?gcgcgagcag?ccatgaccac 840

ggccatgctg?tatggcttgt?cattacgcta?gcttttgcct?tccttttcta?a 891

<210>101

<211>933

<212>DNA

＜213〉aspergillus niger

<400>101

atgggtggtc?gagatgtcgc?cattctcagc?aggcactttg?ctgtgacatc?ctcacaaagt 60

gttaatggcg?ttgtctctgg?gatgttccaa?cacacagtca?cctcttcacc?cagcttcact 120

accaaccaat?tcttcaagaa?gaagttcact?gctgcaattg?ctactgccat?tttcgcaagc 180

gttgccgtcg?cagctcccca?gcgtggcctc?gaggcccgcc?tcaaggcccg?cggcagcagc 240

aagggatccc?gacccctcca?ggcagttgct?agacctgcat?caaccaagaa?ccagaccaac 300

gttgagtaca?gctccaactg?gtccggtgcc?gtgctggtgg?agcctccctc?tgctgcagcg 360

acctacactg?cggtgaccgg?caccttcact?gtccctgagc?ccaccggcaa?ctctggaggc 420

agtcaggctg?catctgcctg?ggttggtatc?gacggtgata?cctatggaaa?cgccattctt 480

cagaccggtg?ttgacttcac?cgtgaccgac?ggagaggcct?cgttcgatgc?ctggtatgag 540

tggtacccgg?attacgccta?cgacttcagc?ggcatcgaca?tctcggcagg?cgatgagatt 600

gttgccattg?tggagtccta?cacctcgact?accggtattg?ccattattga?gaacaagagc 660

accggccaga?aggtgtccaa?ggagctgtcg?tccagctcca?gcctcggtgg?acagaacgct 720

gagtggattg?tggaagactt?cgaggaaaat?ggttcgctcg?tcaacctggt?ggactttggc 780

accgtcacct?tcactggtgc?tgttgccaag?gcggcgggtg?gtgagagtgt?tggacttacc 840

gatgcgacca?tcatcgagat?tgaggagaat?ggccaggttg?tcactgacgt?taccatcgac 900

agcgactctg?aggtgaccat?cacctacgag?taa 933

<210>102

<211>2046

<212>DNA

＜213〉aspergillus niger

<400>102

atgcgctgct?ccctcatctc?ccttctaggc?ctggcggcca?tcccggccct?tggaggctgt 60

cccttcgcac?acactgcgaa?catgggcatt?gataacatgg?tgaaagcaca?cgctcacatg 120

tcccgaccgt?tgattgcctc?caagagcagc?ccctcaactg?ttcctacctc?ctctagcacc 180

ccttctgtcg?ggcagaaagg?cgtgttcatg?atgaaccgca?ttgctcctgg?cacatccgag 240

ctctacattg?ccaacacaga?tggcagtaat?gaacgcccac?tcctctccaa?ccccgtctac 300

gagtaccatg?cctccttctc?cccggatgta?gaatggatca?ccttcaccag?cgagcgcaat 360

ggtgacggta?actctgacat?ctaccgcgta?cggaccaacg?gctccgatct?ccaggaattg 420

gttgccacgc?ctgcagtgga?agactccgtt?gttatctctc?ccaacggccg?cctggcagcc 480

tacgtctcca?ccgccaacaa?catgaaggca?aacatctgga?tccttgatct?tcagaccggc 540

gcgcagtgga?acctcacaaa?tacacccacc?actgccgcca?actcctccct?catggagagc 600

tatctccgtc?ctgcctggtc?tcctgatggc?gaatggatcg?ccttctcttc?ggaccgcaac 660

acccaatggg?acggacacgg?cgtaccgacc?ttcctcggcc?gcacgggctg?ggagacgacg 720

caagaactct?ctctctacgc?catccgtccc?aatggctctg?acttccgtca?gatcatctcc 780

aagccatact?actctcttgg?atctccgaaa?tggtcagcag?acggtaaacg?catcgtctac 840

tacgaaatga?cccgggaaga?cacctacaac?gcccatcgtc?cagaaaccat?taccacagcc 900

aactcgacga?tcatgtccgt?agacttcgag?acaggcaccg?atgtgcgcgt?ggaagtcgcc 960

ggctccggtg?tcaagcaatt?ccctcagtac?ctggacaaga?acggcaccat?cgcctacacc 1020

ctcaaaggcg?gcaccagcga?gggcttctac?acgaccgcgg?gactctacgt?caacacgacc 1080

tcggcgaccc?tcaggtcccc?ggcgtggtct?cccgacggca?agcaagtagt?ctacgaaaag 1140

agcacctgga?gcatccgctc?ggggtacaag?cagctctaca?gctgggacag?tgactgggac 1200

taccgcttca?cggacgtctt?ccctcaggtc?tcgcaccagg?agcgcgtcgc?catcacacag 1260

aagcagctgg?gcaattcgtc?catcgtgacg?ttgaacacaa?ccggaggcga?cttgcaactc 1320

gtctacgacc?ccagcacggc?ggactttgtc?agcgatgacg?aaaccacagg?actgagcgct 1380

taccagccca?gctggtcacc?ctgcggcgag?tggctcgtct?tcggcgtcgg?attctggttc 1440

gagacgagag?aagcctcagg?cggatggatc?gtgcgggcca?ccgccaacgg?gagctactcg 1500

gaggttctcg?tgaacagcag?ctactccatc?accgaggatg?gagccctgaa?cagcgggttc 1560

ccgagtttct?cgccggatgg?caagaaagtg?gtgtatcggg?tttggggagc?cgacactgca 1620

acctacggca?acgccagcga?gatcgggctg?cgggtgctgg?acctcgagac?gcgaaagaca 1680

accgtcctaa?ccacagaatg?ggacaatctg?ccccagttct?ctcccgatgg?agagctcatc 1740

ctattcacac?gcaaaaccag?cacgtacaat?tacgatgtgt?gcacgatccg?gccggatggg 1800

acagatctcc?gcgtgttgac?gagcagcggt?gctaatgatg?cgcatgcggt?ctggtcgcag 1860

gatggacgga?ttatgtggtc?taccggcatg?tatgggttcc?ggtttgagtg?tgcgctgtat 1920

ggtgatacgt?tccagccgta?tgggcaggtt?atgattatgg?atgcggatgg?gggaaataag 1980

aagttgatga?ccaactcgat?gtgggaagat?tcgatgccgt?tgttcttgcc?gagggaggta 2040

ctttag 2046

<210>103

<211>1875

<212>DNA

＜213〉aspergillus niger

<400>103

atgcctccgg?atgcaaaatc?gcctggctac?cagcctggta?tggcagtatt?accatctagg 60

ccacatcctg?ccaagggaaa?agccattcga?ttcctccttt?cccttgcatt?ggtcgcgttt 120

gctattgttc?aattatgtgg?taatttccac?aaaaatagga?gcgttgaaca?acagcttcag 180

agtcaaacac?ttgatgatga?gtcctttaaa?tgggaagatg?ttactcctac?caagcaactc 240

gtataccatc?catgctttgg?tgatcacgaa?tgcgctcgct?tgtcgcttcc?aatgaattgg 300

aaccgaactg?atggtgaagg?gtcaaaaatt?gccttggcgg?ttatcaaact?tcctgccaag 360

gtacctgtca?cagatgcgcg?atatggtggt?gccattcttc?tgaatccagg?tggtcctggt 420

ggatccggag?tgagcatggt?ctttagatac?gggaaagcta?tccagaccat?cgtcgactcc 480

ccagaatcac?caagtgcaga?ttcagcgagc?ggaaagtatt?tcgatgttgt?tagctttgat 540

ccaagagggg?tcaacaacac?aacacctaat?ttttcctgct?tccctgaccc?cgcgacgagg 600

aaagcgtggt?tactgcagtc?agaggcagag?ggtctacttg?ggagttctga?aggagtcttc 660

gatactcgat?gggcaaggta?cgaagctttt?gagcggctac?tttcgacagc?tccgaacact 720

ttcccagttg?gaacaaacgt?tgacgccgag?aggataaggc?tgcacaaccg?ttggaaaaaa 780

ggggaggaga?agctgctata?ctggggcttt?tcctatggga?caatcctggg?ttccacgttt 840

gcggctatgc?agcctcatcg?cataaaccgt?gctgtcatag?acggagtctg?caacgctgat 900

gattattacg?ccggcaactg?gcttaccaat?ttacaagatt?cggatgcagc?attcaataaa 960

tttttcgagt?actgctacac?agctggccca?tcagcgtgtc?cgtttgcgct?cggcggagat 1020

cccgaagatc?tcaagtctcg?ttatgagcag?attttgacca?atcttacatc?gagccctatt 1080

gctgtgtctc?cttctggaaa?taggggccca?gagataataa?cctatagtga?tgtgaagtca 1140

ttggtcgtgc?aagctctcta?tgtgcctttg?aaattattcg?atttggtggc?taggctatta 1200

gctgagctcg?agcaaggtaa?cggctcttca?ttcgctgact?tgaagtatga?agccaaacaa 1260

tggccagtac?cgcctccatg?cgattcctcg?tccacacaat?acaaagtacc?tggcgagagt 1320

gatcaggagg?ccgggaggaa?tatcctatgt?acagatggtc?caggcctcga?cggaactgcc 1380

aaggaggatt?tccggagcta?ctggaatatg?ctccggggac?aaagtaaggc?ggttggagat 1440

ttctgggccg?aggttcgcat?gtcgtgtgtc?aaactggaga?cgcgacctga?gtggcgctat 1500

gatggtatgc?gtatccaagg?gcccttcgca?ggcaatacat?cgcacccatt?gctgtttatc 1560

gggaatactt?atgatccagt?aacgccgcta?cggaatgctc?atacgatggc?gcgtggattt 1620

cctgagtcaa?tcgttctaga?gcagaactct?gtcggacatt?gcacactgag?tggcccatcc 1680

ttgtgtacag?cgaaagcgat?acgccagtat?ttccagaccg?gagagttacc?tgaccccgga 1740

actgtttgcc?aggtagagga?gcttcccttt?cgtcttgccg?gatatgagag?aagtcaggtc 1800

atgtcgccag?gtgacacaga?attgatgtcc?gccttgcatt?cgctgagcga?gttccgccat 1860

ctgctaggcg?cgtga 1875

<210>104

<211>1665

<212>DNA

＜213〉aspergillus niger

<400>104

atgttgagta?gtctgctgct?tgggggtctt?ctgggtctag?cgaccgctca?atttcctccc 60

gagccggaag?gcatcactgt?gctcaagtcc?aagttgcatg?agaatgtgac?tatttctttc 120

aaagagcctg?gaatttgcga?aactacgccg?ggtgtccgat?cttattcggg?ctatgtacac 180

cttccccccg?cctcaaccag?cttcttttgg?tttttcgaag?cccgcaaaga?tcccagcaat 240

gcgcctctgg?ccatctggct?caatggcggt?ccgggtggct?cgtcgctcat?ggggctcctt 300

gaagaattag?gtccttgttc?cattgcatca?gactccaaga?ccacagtcct?caatccttgg 360

agttggaaca?atgaagtcaa?tcttctattc?cttgaccagc?caactcaagt?cggcttctca 420

tacgatgtcc?caacaaatgg?cactttgaca?gctaatggga?ctgcattcgc?ggctcacgct 480

ctatggcatt?tcgcgcaaac?ctggtttttc?gagttcccac?actacaagcc?aaacgatgat 540

cgtgtcagtc?tctgggctga?aagttacgga?ggccattatg?gtccaggcat?ctttcggttc 600

ttccaacagc?agaatgacaa?aatcgcagag?gggactgcag?aagacggtgc?acagtatttg 660

catctcgaca?cgcttggcat?tgtgaacggc?ttgatggata?tggtgatcca?agaagaggct 720

tacattactt?ggccatacaa?taacgtaagg?ctcgcccctt?cttcattcaa?ctcgcgaggc 780

tttcgcgatc?aggccctcgc?ctgcgaagcg?gctttgaaag?aacgcgattc?cggcttgcct 840

cactcaggga?agaatatctc?tgaaatttgc?ggaggccttg?cactagaatg?gggagatggc 900

cccatcacct?actaccacac?cttcaatcgc?gggtggtacg?acatcgccca?tcctaagaac 960

gacccattcc?ctgccaagca?catgctcgga?tatttgacgc?aggagtccgt?ccttgccgct 1020

cttggggtac?cagtcaattt?cacatcgtct?tcgagtgccg?tggctacaca?gttcataaaa 1080

acctttgata?tcgtccacgg?cggcttcctg?gatgcaattg?gctacctcct?cgacagtggt 1140

gtaaaagtac?acatgatgta?cggagatcgt?gattacgcct?gcaattgggt?cgggggcgaa 1200

aaagccagcc?ttgcagttcc?gtattcccgt?atcaccgaat?ttgccgacac?gggatactcc 1260

ccactcctta?cgcccgacgg?gatcagcggc?atgacccgcc?agctgggcaa?ctacagcttc 1320

actcgcgtct?tccaagccgg?gcatgaggtc?ccctcctacc?agcctgtcgc?ggcgtatgag 1380

atcttcatgc?gggcgacatt?caacaaagat?atccctactg?gcctcttggc?tgttgatgac 1440

gaattccagt?cggttggacc?taaggatacg?tggcatatca?agaatatccc?tcctattatg 1500

ccaaagccgc?agtgctatgt?tctaagtccc?ggcacgtgta?ccccggaggt?ttgggagacc 1560

gttttgaacg?gatccgcgac?ggtaaaggat?tggtatgtcg?tggatgatag?cgcgggtgtt 1620

gaggaccacg?aggggttcag?cattcttgga?ggggatgagt?tgtag 1665

<210>105

<211>1737

<212>DNA

＜213〉aspergillus niger

<400>105

atgaccaggt?ttcaattgct?tccccttgtc?gcagggctgc?ttgccccttc?aattgcagcc 60

cttagcatcc?cttccccgca?gcagatcctc?gattctctca?ctttcggaga?gcacaccgac 120

ggcttttgtc?cgctggcacc?caaggttgag?gttcctgacg?atggtttctt?tccagctctc 180

aagttcgtag?aagatgcctc?gttcaagtcg?cgccaagtca?atcgtctctc?cagggcggtt 240

caagttccga?ccgcaatcga?cgactacatg?aaggatccct?acgacgaaaa?gttcgcccca 300

ttcctcgact?tccagaagct?cctgcagacc?ctctttcccc?tcacccactc?ctacgcccgc 360

gtagatcaca?tcaaccgatt?tggtctcgtc?ttcaccctca?atggcacaga?tgactcgctc 420

aagcccctgc?tattcaccgc?gcaccaggac?gtcgtgccca?tcaacgaccc?tgccgactgg 480

acctatcccc?ccttcgatgg?ccactacgac?ggcgaatggc?tctggggccg?cggtgccagc 540

gactgcaaga?acgtcctgat?cggtctcatg?tccgttgttg?aagacctact?ctcccaaaag 600

tgggagccaa?cccgcacagt?cgtcctggcc?ttcggattcg?acgaagaatc?ccacggcttc 660

ctcggcgccg?gatccatcgc?caaattcctt?gagaagaaat?acggaccgga?cagcttcgaa 720

tttatcctcg?acgaaggcgg?catgggcctc?gaagttctag?acgacaacaa?caacggcgtc 780

gtctacgctc?tccccggcgt?tggcgaaaag?ggcagcatcg?acgttgtgct?cactctggcc 840

gtaccaggcg?gccacagctc?cgtgccccct?ccacacacgg?gaatcggcat?catcgccgag 900

atcatctatg?agctagaacg?ccaggacctc?ttcgtccccg?tcctagacac?tcaccacccg 960

acccgcaaga?tgctcgaatg?ccaagtccgc?cactccccct?cgcaagtcga?accgtggctc 1020

gcctccgccc?tccaatcaag?cgactacatc?tccctagcag?agaaactggc?ctcctcgcgc 1080

ggcgacaagt?tccgcttcat?cctccaaacc?tcccaagcag?cggacatcat?caacggcggc 1140

gtcaaatcca?acgctctccc?cgagaaaatc?aacgccctcg?tcaactaccg?catcgctctg 1200

caccaaaccc?cagacgatat?caagaaccgc?gctgtggaga?tcatctctcc?catcgtcaag 1260

aaatataacc?tctccctcac?ggccttcccg?gaaagcgaca?ccgttgaccc?ctccctcaac 1320

aaccacctca?cccttactac?cctcagcggc?gccctcagtc?ccgccccggt?cagcccaacg 1380

gacatcgaca?ccgacgccgt?ctgggcccgt?ttctcgggcg?tcactcgctc?ggtcttcgaa 1440

tctgtcccta?gtctcgaggg?cagaaaggtc?gtcgtgagcg?gcgacatcat?gaccgggaat 1500

acggatacga?gattctactg?ggctttgtcg?aggaatattt?acaggtggag?tccgtcgagg 1560

gcgggtaaag?cgctgaatat?tcatactgtt?gatgagagga?tcgatattga?tattcatctt 1620

gaggcgatga?tgctgtatta?cgatcttatt?cgctctttcg?atggacggac?cgattcatct 1680

gtcatttctg?ctgcgtcggc?agctgctgat?gatgaacttg?ctcacgacgt?gctgtga 1737

<210>106

<211>1371

<212>DNA

＜213〉aspergillus niger

<400>106

atgaagagca?ccactcttct?ttccttggcc?tgggctgccc?agtccgccta?ttccctctct 60

atccacgagc?gcgatgaacc?cgctactctt?cagttcaact?ttgaacgtcg?tcagatcgcc 120

gaccggtccc?gtcggaagcg?atcgacggcc?tcggccgacc?tcgttaacct?ggctacgaat 180

cttggctaca?cgatgaacct?cacactcggc?actcccggcc?aggaagtcag?tgtgacgttg 240

gacaccggca?gcagcgatct?ctgggtcaat?ggggccaact?cgtccgtctg?cccctgtacc 300

gattacggct?cttacaactc?aagcgcttct?tccacctaca?ccttcgtgaa?cgatgagttt 360

tatatccagt?atgtcgacgg?cagtgaagcc?acaggcgact?atgtcaacga?tactctaaag 420

ttctccaatg?tgactttgac?gaactttcaa?tttgccgtcg?catatgacgg?cgactccgag 480

gagggggtcc?tcggtatcgg?atacgccagc?aatgaagcca?gccaggccac?cgtcggtggt 540

ggtgaataca?ccaacttccc?cgaagccctc?gtcgatcaag?gcgcgatcaa?ctggccggcc 600

tacagtctat?ggctcgatga?cctcgacgaa?ggaaaaggca?ccattctgtt?cggcggagtc 660

aacaccgcca?agtactacgg?cagcctgcag?accctgccta?tcgtctccat?cgaagacatg 720

tacgtcgagt?tcgcggtcaa?cctgacggcc?gtgcaccttg?agaagaacgg?caactccgtc 780

tcggtcaaca?acagcgccac?gcaattcccc?atccccgccg?tgctggacag?cggcacggcc 840

ctgacctaca?tcccgacctc?cgccgcagcc?agcatctacg?aggccgtcgg?tgcccaatac 900

ctgagcgagt?acgggtacgg?agtgatcgag?tgcgacgtca?aggacgaaga?cttcaccttc 960

ctgttcgact?ttggatcctt?caacatgagc?gttgacatca?gcgagatgat?cctcgaggcc 1020

agttccgaca?tgaccgacat?gaacgtttgt?acgtttggcc?tcgcagtgat?cgaaaatgag 1080

gccctgctgg?gcgatacctt?cctgcgcagc?gcatacgtcg?tctacgatct?cggaaacaac 1140

gagatctccc?tggccaaggc?caacttcaac?cccggcgagg?accacgtcct?ggagatcggc 1200

accggatcgg?atgccgtgcc?caaggcgacg?ggggcgacgg?cgaccggcgc?ggcagccaca 1260

tccacggcct?cgagcgacaa?gtcggacaag?gagagttcgg?ctacagtgcc?gcgcagccag 1320

attgtctcgc?tggtggcggg?agtcttggtc?ggtgttttct?tggttctgta?a 1371

<210>107

<211>1995

<212>DNA

＜213〉aspergillus niger

<400>107

atgttggtcc?gtcagcttgc?cctggctctg?gccattgcgg?ccttgtccga?tgccattccg 60

acatccatca?agcatgtcct?gcacgagaaa?cgtcacaagc?ccgcatccga?ctgggtgaag 120

ggtgcgcgcg?ttgagagcga?tgcggtcctg?cctatgcgca?ttggccttgc?ccagaacaac 180

ttggacaagg?gctatgactt?cctgatggaa?gtatcggacc?ccaagtcttc?caaatacggc 240

cagtactggt?cggcagacga?ggtgcacgac?atcttttcgc?catccgagga?ggctgttgag 300

gcagtgagag?aatggcttgt?cgcctctggt?atccatccgt?cgcgggtggt?gcactccgac 360

aacaagggct?ggctcgcgtt?cgacgcctac?gcccatgaag?ccgagaggct?gttcatgacg 420

gaattccacg?agcacgagag?cgaccgaagt?gctaagatca?gggttggatg?cgaccaatac 480

cacgtccccg?aacacatcca?gaagcacatc?gactacatta?cccctggagt?gaagctcacc 540

caggtcgtga?agaggaccaa?caaagtcaag?cgtgcttccc?aactagctca?ctcttccaag 600

gccaagtctg?ctgcccaagg?tccgcagcca?ctccccaaca?aggccaagtt?cctgcctgaa 660

gacctccgcg?gctgcggtta?caacatcacc?ccctcgtgta?tcaaggcctt?gtatcagatc 720

ccagacgcta?agacggcgac?cccgaacaac?agcctgggtc?tgtacgagca?gggtgactac 780

tttgccaagt?ccgacctcga?cctcttctat?aaggagtatg?cgccgtgggt?tccccagggt 840

acctatccca?tcccagccct?gattgatggc?gccaattact?cggttccttc?ctacagctcc 900

ctgaacacgg?gtgaatccga?cattgacatt?gacatggcct?actccctgct?ctaccctcag 960

caggtgaccc?tctaccaggt?tgacgaccag?ctctacgaac?cagtcgaggt?cgacacaacc 1020

aatctgttca?acaccttcct?cgacgctctc?gatggctcct?actgcaccta?cagcgcctac 1080

ggcgagaccg?gcgatgaccc?gtcgatcgac?cccgtatacc?ccgacacccg?ccccggcggc 1140

tacaaaggaa?agctccagtg?cggcgtctat?aagcccacta?acgtaatcag?cgcctcctac 1200

ggccaatccg?aagccgacct?ccccgtcagc?tacaccaagc?gccaatgcaa?tgagttcatg 1260

aagctcggtc?tacagggaca?ctccatcctc?ttcgcgtctg?gcgactacgg?cgtcgcgtct 1320

ttcgccggcg?acggtgacga?gaacggctgt?ctcggcccag?agggcaagat?cttcaacccc 1380

cagtacccct?ccaactgccc?ctacgtcacc?tccgttggag?gtaccatgct?gtgcggctac 1440

cagaccgtca?acgacagcga?gagcgtcatg?cacgttaacc?ttggcggaac?cgcaagtaac 1500

ttcagcactt?ctggtggctt?ctcgaattac?ttcccccaac?cggcatatca?gtttgctgct 1560

gtggagcaat?acttccggtc?tgcgaacctg?tcgtatccgt?attactcgga?gtttgaggtc 1620

gatgttaaca?cgaccaaggg?tctctacaat?aggcttggtc?gtgcttatcc?ggatgtctcg 1680

gcgaatggag?cgcatttccg?cgcttatatg?gatggatacg?attatcattg?gtatggatcg 1740

agtttggcgt?cgcctttgtt?cgcgtcggtt?cttactttgc?tcaacgagga?acgcttcgct 1800

atcggcaagg?gccccgtggg?attcgtgaat?cccgtgcttt?atgcttatcc?gcaagtgctg 1860

aacgatatca?ctaatggtac?taatgctggg?tgtggaactt?atgggtttag?tgctattgag 1920

ggatgggatc?ccgctagtgg?tttgggtacg?cctaactacc?cattgatgaa?ggagctgttc 1980

ctctctttgc?cttag 1995

<210>108

<211>1563

<212>DNA

＜213〉aspergillus niger

<400>108

atgcgggtta?ccacggcaat?tgcttcatta?ctactggtcg?gctcggccac?cagtctccaa 60

aatcctcatc?gtcgggctgt?tccgccccct?ctctcgcatc?gcagcgtagc?gtctcgctcc 120

gtgcccgttg?agcgccgaac?caccgacttt?gagtatttga?ctaacaagac?tgcaagattc 180

ctggtcaatg?gcacaagcat?ccccgaagtc?gatttcgacg?tcggcgagtc?ctacgccggc 240

cttctcccca?atacgcccac?tggcaattct?agcctattct?tctggttctt?cccctcgcaa 300

aatccagagg?ccagcgatga?gatcaccatc?tggctcaacg?gcggccccgg?atgtagctcc 360

ctagacggcc?tgcttcaaga?gaacggccca?ttcctctggc?agcctggcac?ttacaagccc 420

gttcctaatc?catactcatg?gaccaacctc?accaatgtgg?tttacatcga?ccaacccgcc 480

ggcacaggct?tctccccggg?cccctcgacc?gtaaataacg?aggaagacgt?ggctgcccag 540

ttcaacagct?ggttcaagca?cttcgtcgac?accttcgacc?tgcacggccg?caaggtctac 600

atcaccggtg?aaagctacgc?gggcatgtac?gtcccctaca?ttgccgatgc?catgctgaac 660

gaggaggata?caacctactt?caacttgaag?ggtatccaga?tcaacgaccc?gtccatcaac 720

agcgactcgg?tcatgatgta?ctcccccgcc?gtccgccatc?tgaaccacta?caacaacatc 780

ttccagctaa?actccacttt?cctctcctac?atcaacgcca?aagccgacaa?gtgcggctac 840

aacgccttcc?tcgacaaagc?catcacctac?ccacccccca?gtcccttccc?caccgcccct 900

gaaatcaccg?aagactgcca?agtctgggac?gaagtcgtca?tggccgccta?cgacatcaac 960

ccctgcttca?attactacca?cctgatcgac?ttctgcccct?acctctggga?cgtgctcggc 1020

ttcccctccc?tcgcctccgg?cccaaacaac?tacttcaacc?gctccgacgt?ccagaagatc 1080

ctgcacgtcc?ctccaacgga?ctactccgtg?tgctcggaga?ccgtcatctt?cgcgaacggc 1140

gacggcagcg?accccagctc?ctggggtccc?ctacccagcg?tcatcgaacg?cactaacaac 1200

actatcatcg?gccacggctg?gctcgattac?ctcctcttct?tgaacggctc?gctcgccaca 1260

atccagaaca?tgacctggaa?cggtaagcaa?gggttccagc?gtcctcccgt?ggaaccgctc 1320

ttcgtccctt?accattatgg?tctggctgag?ctgtactggg?gcgatgagcc?tgacccgtat 1380

aaccttgatg?ctggcgctgg?atacctgggt?acagcgcata?ccgagcgcgg?gttgactttc 1440

agctcggtgt?atttgtctgg?tcatgaaatc?ccgcagtatg?ttcctggtgc?ggcttaccgc 1500

cagttggagt?tcctgctcgg?taggattagt?agtctttcgg?cgaaggggaa?ctatacctct 1560

tga 1563

<210>109

<211>1656

<212>DNA

＜213〉aspergillus niger

<400>109

atgcgtggct?ctcggttggt?gctcttgttg?cccctggctg?cacttagttg?tgctatgccc 60

gagaatgaat?ggtcatctac?gataagaagg?cagttaccaa?aagcgtccac?tggcgtcaaa 120

tcgataaaaa?ccccaaacaa?tgtcactatc?aggtataaag?aaccaggaac?cgaaggaatt 180

tgtgagacaa?cacctggggt?caaatcatac?tccggatatg?tcgatctttc?gccagagtcg 240

catactttct?tttggttttt?cgagtcacgc?cgtgaccccg?aaaatgatcc?agtgactctg 300

tggctgaatg?gtggccctgg?aagcgattcc?ttgattgggc?tttttgaaga?gttgggtccg 360

tgtcacatca?caccagagta?cgaatcaatc?atcaatcagt?actcctggaa?cgaggtcacc 420

aatcttcttt?tcttgtctca?gcccctcggt?gtggggttct?cttacagtga?aaccgaggcc 480

gggtccttga?atccatttac?tggagccgtc?gagaacgcct?cctttgctgg?agttcagggt 540

cgatacccag?ttattgatgc?cactatcatc?gacacgaccg?atatcgctgc?acgcgcaacc 600

tgggaggtgc?ttcagggctt?cctcagtggc?ctgtcgcagc?tagattccga?agtcaagtcc 660

aaggagttca?acctgtggac?agagagttac?ggaggacact?atggaccagc?gttcttcaat 720

catttctacg?agcaaaattc?gaagatcgct?agcggggaag?tcaatggcgt?ccaactgaat 780

tttaactccc?tcgggattat?caacggcatc?attgatgccg?cgattcaggc?agactactac 840

gcagactttg?ccgttaataa?tacatatgga?atcaaagctg?tcaatgacac?agtgtacaac 900

tatatgaagt?tcgccaacac?gatgccaaat?ggatgccagg?atcaggttgc?ttcgtgtaaa 960

ttgaccaata?ggacctcgct?ttctgattat?gctatatgta?cagaagcagc?caatatgtgc 1020

agggacaatg?tcgaagggcc?ttactaccag?tttggcggcc?gtggcgtgta?tgatattcgg 1080

cacccctaca?atgacccgac?cccgccgtcc?tactttgttg?actacctcaa?gaaagactca 1140

gtcatggatg?ctatcggcgt?ggacattaac?tacaccgagt?ccagcggcga?agtatattat 1200

gcattccagc?agaccggcga?ctttgtatgg?ccgaatttca?ttgaggacct?cgaagagatc 1260

ctccaactcc?ccgtacgcgt?gtcgttgatc?tacggcgatg?ccgactatat?ctgtaactgg 1320

ttcggcggtc?aggccatctc?actcgcagtt?aactaccccc?atgcagctca?gttccgtgca 1380

gcgggataca?cacccatgac?agtagatggg?gtcgaatacg?gtgagactcg?cgagtatggc 1440

aacttttcgt?tcacccgcgt?atatcaggct?gggcacgagg?ttccatacta?tcaaccgatc 1500

gcagcgttgc?agctgttcaa?ccgtacttta?tttggatggg?atattgcagc?gggtacaact 1560

cagatttggc?ccgaatatag?caccaacggg?acatcgcagg?ctacacacac?ggagtcgttc 1620

gtgccactgt?ccacggcgtc?gagtaccgtc?aattag 1656

<210>110

<211>1872

<212>DNA

＜213〉aspergillus niger

<400>110

atgccttttc?ccttttcgtc?cgctcttctc?ggctatatct?taactacgag?cactactctc 60

acctccctag?tcgcaggaca?gtattaccct?ccgacgcctg?aggatctcac?cgttattcat 120

tcggagatat?tccctggtgc?gaggatctcc?tataagcaac?ccctcggcat?ctgcaccacc 180

accccctcca?cccccagcta?ctccggctac?atccacctcc?ccccacacac?ccttaccaat 240

ctctccattc?caggaatcag?catctcgcaa?ccatacccta?tcaatacctt?tttctggtac 300

tttccttccc?gccatcacca?caacaatgat?acatccccac?tcaccatctg?gatgaacggc 360

gggcccggcg?gatcctccat?gattgggcta?tttcaagaga?acgggccatg?tactgtgaat 420

acggactcga?attccacggc?ctataatccc?tggtcgtgga?atgagtacgt?cgatatgttg 480

tatattgagc?agccggtgca?gacgggattt?agttatgatg?tgttgaggaa?tgggacgtta 540

gatttgaatg?agacgttttt?ggtggggacg?ttgccgagtc?aggatgtgca?tgggacggtg 600

aatgggacgg?ttaatggggg?aagggcgctt?tgggttgcgt?tgcaggtttg?gttgggtgaa 660

ttctctgaat?atgtttcttc?tgttgacggg?aatggtggtg?gtgatgacag?ggtgagtata 720

tggacggagt?catatggggg?acggtatgga?ccggcataca?cggcgctctt?tcaggagatg 780

aatgagagga?ttgagagtgg?ggaggtaagc?accgggaaga?agatccattt?ggatacgctg 840

ggcattatca?atgggtgtgt?ggatttactc?gtgcaggtcc?cttcgttccc?tgagcaggcg 900

tataacaata?cgtatgggat?cgagggaatc?aatcgcacgc?tctacgaccg?ggctatggat 960

agttggagca?agcctggcgg?gtgcagggat?atgatcatcg?agtgtcgcga?tgctggcgag 1020

ctcggagatc?ccctcatcat?ctgcgaggag?gcgtcggact?actgttcgcg?ggagatcaag 1080

agcctgtata?cggatacctc?cgggcgagga?tactacgaca?tagcgcattt?cacgccggat 1140

gcagctctcg?tgccttactt?cgtcgggttc?ttgaatcgcc?catgggtgca?aaaggcactt 1200

ggggtcccgg?tgaactatac?catgtcgtca?gaggcagtgg?ggaacagttt?cgcctcgacg 1260

ggcgattatc?cgcgaaatga?tccccgcgga?atgatcgggg?atattggata?cttgcttgac 1320

tccggtgtca?aggtggctat?ggtatatggg?gaccgggact?atgcttgtcc?gtggcgcggc 1380

ggggaagatg?tcagcctgct?ggtggagtac?gaggatgcgg?agaagttccg?tgctgctggg 1440

tatgccgaag?tgcagacgaa?gtcatcctac?gttgggggtc?tagtaaggca?gtatgggaac 1500

ttctcgttca?cgcgtgtctt?tcaggcgggc?catgaggtgc?cattttatca?gcccgaaacg 1560

gcgtatgaga?tttttaatcg?cgctcagttt?aattgggata?ttgcgacggg?aggcatttct 1620

ctggagcaga?atcagagcta?tgggacggag?ggaccgtcgt?caacgtggca?tatcaaaaac 1680

gaagtgccgg?agagccctga?gccgacgtgc?tatttgttgg?cgatggattc?gacttgtacg 1740

gatgagcaga?gggaacgggt?gctgagtggg?gatgcggtgg?tgagggattg?ggttgttgtt 1800

gatgatattg?aggctgaaag?ctcgttcagc?ggtgttggtg?atcagctggc?acaggtccct 1860

ttgggacatt?ga 1872

<210>111

<211>1320

<212>DNA

＜213〉aspergillus niger

<400>111

atgagaacat?ctactctttt?gctcctctgg?agcactgcag?gagcagcttt?ggcttctccg 60

tacccgcttc?ccgactcgca?agtagtcttc?gccgcggatc?acgaggtccc?gaatacacag 120

ggcaaacacg?tcgtggacga?ggccatactc?tcggcgctga?acgctcattc?tgacccagtc 180

gctgcaatgg?tgtctctacg?tcccgaaact?gcagcttttc?tagctgaacc?tcgtctcttg 240

cacattcggg?gcgaagagaa?ggcggaatgg?atgaccgaag?gcgacaagct?gcgcctccgc 300

caacgcggaa?agaagttcat?ggacattacc?gagcatcagg?acttctacgc?agagcaggcg 360

atggcttcgt?ttgctgggga?tcctaatctt?cccaagctgt?cccataaagg?tctcgtcaag 420

ccgctgttct?ctcaaatcga?gacggaacga?atgcacgata?tcctgcagca?catgacctcc 480

tactacaatc?gatactacgg?tgattatcac?ggcgagatga?gctccgaatg?gctgcacgac 540

tacattgctg?cgatcatctc?caaatcgcct?ttccgcaccc?acatctctct?cgaatacttc 600

acccatcctt?tccgccaatc?ttcaattatt?gcacgcttcg?agcctaaagt?tcgcagcttc 660

tcccaacctt?tgaccatcat?tggtgcgcac?caagattcgg?ccaattatct?ttttcccctg 720

ctgcccgccc?ctggcgctga?cgatgactgt?tccggcactg?tcagtatcct?cgaggccttc 780

cgcgttctgg?cggagaatgg?ctacacgccc?aaggacgggc?ctgttgaatt?ccattggtat 840

gcggctgaag?aggccgggct?actgggcagc?caagccatcg?cgcggtacaa?gaaggagcag 900

ggcgctaaaa?ttgatgccat?gatggagttt?gatatgacgg?cttttattgc?ccgtaacgcc 960

accgagacca?tcgggtttgt?tgcaacccaa?gccgatgcag?cgctcacaaa?ctgggccctc 1020

aacctcagtc?gagaatacat?ctccattccg?gcggaagtct?atgaacttgg?ccccaacgct 1080

ggatccgact?acatgtcata?cactaagctc?aactaccccg?ctgcctttgc?atccgaaggc 1140

aacccgctcg?ctgggggctc?tttcccgggt?gaaatggacc?cctacgtaca?cggcatcaag 1200

gataggatgg?acgttgacga?tgaaacgggc?gtcttctcta?tcgaacacat?ggctcggttc 1260

tccgagttgg?ctatcgcatt?tgttgtcgag?caggctgggt?gggataatac?atggcggtag 1320

<210>112

<211>1581

<212>DNA

＜213〉aspergillus niger

<400>112

atgcgttcct?tctccgttgt?cgctgccgcg?tcactggcgc?tctcttgggc?gtctctggcc 60

caggctgctc?gcccccgtct?tgtgcccaag?cctatctctc?ggccagcttc?gagtaagtcg 120

gctgcgacta?cgggtgaggc?ttattttgag?cagctgctgg?accatcacaa?cccggagaag 180

ggaacgtttt?cccagcggta?ctggtggagt?actgaatact?ggggtggacc?tgggtcaccg 240

gtggtcctct?ttaaccctgg?agaggtctct?gccgatggct?atgaggggta?tctcaccaac 300

gatactctca?ctggtgtcta?tgcgcaggag?atccagggtg?ccgtcattct?cattgaacac 360

cgctactggg?gcgactcttc?gccttatgag?gtgctcaatg?ccgaaacact?tcagtatctc 420

acactggatc?agtccattct?ggacatgacc?tacttcgccg?agacggtaaa?gctgcagttc 480

gataatagca?gccgcagcaa?tgcgcagaat?gctccctggg?tcatggtcgg?tggctcatac 540

agcggtgcct?tgacggcttg?gaccgagtct?atcgcgcctg?gaacgttctg?ggcttaccat 600

gccaccagtg?cgcctgtgga?ggctatctat?gacttttggc?aatacttcta?ccccattcag 660

caaggtatgg?cacagaactg?cagcaaggat?gtgtctctgg?tagccgagta?tgtcgacaaa 720

attgggaaga?atggaactgc?caaggaacag?caggagctca?aagaattgtt?tggtctggga 780

gctgttgagc?attacgatga?ctttgccgct?gtcctgccca?acggaccgta?cctctggcaa 840

gacaacgact?ttgtcacagg?atactcttcc?ttcttccagt?tctgtgatgc?tgtcgagggt 900

gtcgaagccg?gcgcggcagt?gacccccggc?cccgagggcg?tcggacttga?aaaggccctg 960

gccaactacg?caaactggtt?caattcaacc?atactcccta?actactgcgc?aagctacggc 1020

tactggaccg?acgaatggag?cgtcgcctgt?ttcgacagct?ataatgcctc?gagccccatc 1080

ttcaccgaca?cctccgtggg?taaccctgtc?gaccgccaat?gggaatggtt?cctctgcaac 1140

gagcctttct?tctggtggca?ggacggtgcc?cccgagggaa?cctccactat?tgtgccccgg 1200

ctcgtcagcg?cctcctactg?gcaacgccaa?tgcccgctct?acttccccga?agttaacggc 1260

tacacgtacg?gcagcgcgaa?gggtaaaaac?tccgctacgg?tgaacagctg?gacgggtgga 1320

tgggatatga?cccgcaacac?gacgcggttg?atctggacga?acgggcaata?tgacccctgg 1380

cgcgactccg?gtgtgtcgag?cactttccgg?cccggtggtc?cgctggttag?cacggcgaac 1440

gaacccgtgc?agattattcc?gggcgggttc?cattgctcgg?acttgtatat?ggaggattac 1500

tatgcgaatg?agggtgtgag?gaaggtggtt?gataatgagg?tgaagcagat?taaggagtgg 1560

gtggaggagt?attatgcttg?a 1581

<210>113

<211>1275

<212>DNA

＜213〉aspergillus niger

<400>113

atgcagctcc?tccagtccct?cattgttgcc?gtttgcttca?gctacggcgt?cctctcctta 60

ccccatggcc?cgtcaaacca?gcacaaagca?cgttccttca?aggttgaacg?ggtccgtcgt 120

ggaaccggtg?ctctgcatgg?gcccgctgct?ctccgcaaag?cataccggaa?gtacggaata 180

gctcccagca?gtttcaacat?cgatctggca?gactttaaac?ccattacgac?aacccatgct 240

gctgctggga?gcgagattgc?agagcctgat?cagactggcg?ctgtcagtgc?tacttccgtc 300

gagaacgatg?ccgagttcgt?ttcgcctgtt?cttattggcg?gccagaagat?cgtcatgaca 360

tttgacactg?gttcttctga?cttttgggtg?ttcgatacga?atctcaatga?aaccttgacg 420

ggacacacgg?agtacaaccc?ttcgaactcc?tcgaccttca?agaagatgga?cggatacacc 480

ttcgatgtct?cgtatggtga?cgactcgtac?gcctctggcc?ccgtcggaac?ggataccgtc 540

aacattggcg?gcgccattgt?caaggagcaa?gccttcggtg?tccccgacca?ggtatcccag 600

tcgttcatcg?aggacacgaa?ctccaacggc?ctggtcgggt?tgggcttttc?ctccatcaac 660

accatcaaac?cggaggcgca?agacacgttc?ttcgccaatg?tcgcaccaag?tctggacgag 720

cccgtcatga?ccgcctcgct?caaggctgac?ggagtgggcg?agtacgagtt?cggcacgatc 780

gacaaagaca?agtaccaggg?caacattgcc?aacatcagcg?tggactcatc?gaacggatac 840

tggcagttct?ccactcccaa?gtactccgtg?gcagacggag?agctgaagga?cattggaagc 900

ttgaacacct?cgatcgcgga?caccggtacc?tcccttatgc?tgctggatga?agacgtggtt 960

actgcctact?atgcgcaagt?tcccaactcg?gtctacgtga?gcagtgccgg?tggttacatc 1020

tacccctgca?acaccactct?tcccagcttc?tcgcttgtcc?tcggcgagtc?gagcctggcc 1080

acgatccccg?gtaacctgat?caatttctcc?aaggttggca?ccaacaccac?caccggacag 1140

gccttgtgct?ttggcggcat?tcaatccaac?ggaaacacct?cgctgcagat?tctgggcgat 1200

attttcctga?aggccttttt?cgttgtcttc?gacatgcgcg?gcccctcgct?tggtgttgcc 1260

tctcccaaga?actag 1275

<210>114

<211>1647

<212>DNA

＜213〉aspergillus niger

<400>114

atgcgcattg?actccgcggc?gctacatctg?gtcccagtcc?tcctgggcca?ggtcggtgct 60

ttacaattac?ccttggtcca?agactccaat?tcacagtggc?agaaaccaaa?tgcaggtgat 120

aaacccctaa?ttagctctcc?gttgcttcaa?gagcaggtca?aggcggagaa?tctgttggac 180

agggcccggc?agctttacaa?gattgcggag?ctgggagaag?acgagtataa?ccaccccact 240

cgcgtcattg?gcagtaaagg?tcaccttggc?acgctcgact?acatatactc?cacccttacc 300

gacctcggtg?attattatac?tgtcgtcaat?cagtccttcc?ctgccgtgag?cggtaatgtc 360

ttcgagtctc?gccttgtcct?tggtcacgat?gttcccaagt?cagctacacc?aatgggtctc 420

actcccccaa?cgaggaataa?ggagccggta?tatggctccc?tggttgctgt?atccaacctc 480

gggtgtgagg?cctcggacta?ctcgtccaac?ttgaaaggcg?ccgttgcatt?tatcagtcgg 540

ggaagctgtc?cgttcgggac?caagtctcaa?ttagctggta?aagcgggagc?tgttgctgcc 600

gtcatctaca?acaacgagcg?gggtgaccta?agcggaactc?taggaaaccc?aacccccgat 660

catgttgcta?cctttggtat?ctcagacgag?gatgctgccc?cagtcctgga?gaagttgaat 720

aaaggcgaga?aggtggacgc?tatcgcctac?gttgatgcga?tagtagagac?catccacacc 780

accaatatca?tcgcgcagac?cacggatggt?gacccgaaca?attgtgtaat?gctgggtggc 840

cacagtgaca?gcgtggccga?gggcccgggt?atcaatgacg?acgggtccgg?tactctgacc 900

cttttggagc?ttgccacatt?gctcacccag?ttccgtgtca?acaactgcgt?gcgatttgct 960

tggtgggccg?ccgaggagga?aggccttctc?ggatctgact?attacgtgtc?cgttctcaca 1020

ccggaagaga?accgcaagat?ccgcttgttc?atggactacg?acatgctcgg?ctcgccgaac 1080

tttgcgtacc?aagtttacaa?tgccactaat?gctgtgaacc?ccgagggatc?tgaggagctt 1140

cgtgatctgt?acaccgactt?ttacgaagat?catgggttca?actacacgta?cattccgttt 1200

gacggacgca?gcgactatga?tgccttcatt?cggcatggta?tcccgggtgg?tggcattgcc 1260

acgggagcag?agggtatcaa?gactgtcgag?gaagcggaca?tgtttggtgg?ggttgctggc 1320

caatggtatg?acccgtgtta?ccatcagatc?tgcgatacgg?tggccaatgt?gaacttgact 1380

gcgtgggagt?ggaacaccaa?gctcgttgcc?cactccattg?cgacttacgc?caagtccttt 1440

gacggattcc?cggaacggtc?cgatgaaccc?atcagccctg?ctgcttttga?ggaaccgaag 1500

taccatggcc?acgcgttgca?attacttcgc?ggtaatacta?cagggaccca?gagcgtcctg 1560

tggggagccc?aaatccagaa?tggaacagct?gcatcggtgc?ttaatctatt?gtccatacga 1620

cgcagaggca?ctttcagtct?aagctaa 1647

<210>115

<211>480

<212>PRT

＜213〉aspergillus niger

<400>115

Met?His?Leu?Pro?Gln?Arg?Leu?Val?Thr?Ala?Ala?Cys?Leu?Cys?Ala?Ser

1 5 10 15

Ala?Thr?Ala?Phe?Ile?Pro?Tyr?Thr?Ile?Lys?Leu?Asp?Thr?Ser?Asp?Asp

20 25 30

Ile?Ser?Ala?Arg?Asp?Ser?Leu?Ala?Arg?Arg?Phe?Leu?Pro?Val?Pro?Lys

35 40 45

Pro?Ser?Asp?Ala?Leu?Ala?Asp?Asp?Ser?Thr?Ser?Ser?Ala?Ser?Asp?Glu

50 55 60

Ser?Leu?Ser?Leu?Asn?Ile?Lys?Arg?Ile?Pro?Val?Arg?Arg?Asp?Asn?Asp

65 70 75 80

Phe?Lys?Ile?Val?Val?Ala?Glu?Thr?Pro?Ser?Trp?Ser?Asn?Thr?Ala?Ala

85 90 95

Leu?Asp?Gln?Asp?Gly?Ser?Asp?Ile?Ser?Tyr?Ile?Ser?Val?Val?Asn?Ile

100 105 110

Gly?Ser?Asp?Glu?Lys?Ser?Met?Tyr?Met?Leu?Leu?Asp?Thr?Gly?Gly?Ser

115 120 125

Asp?Thr?Trp?Val?Phe?Gly?Ser?Asn?Cys?Thr?Ser?Thr?Pro?Cys?Thr?Met

130 135 140

His?Asn?Thr?Phe?Gly?Ser?Asp?Asp?Ser?Ser?Thr?Leu?Glu?Met?Thr?Ser

145 150 155 160

Glu?Glu?Trp?Ser?Val?Gly?Tyr?Gly?Thr?Gly?Ser?Val?Ser?Gly?Leu?Leu

165 170 175

Gly?Lys?Asp?Lys?Leu?Thr?Ile?Ala?Asn?Val?Thr?Val?Arg?Met?Thr?Phe

180 185 190

Gly?Leu?Ala?Ser?Asn?Ala?Ser?Asp?Asn?Phe?Glu?Ser?Tyr?Pro?Met?Asp

195 200 205

Gly?Ile?Leu?Gly?Leu?Gly?Arg?Thr?Asn?Asp?Ser?Ser?Tyr?Asp?Asn?Pro

210 215 220

Thr?Phe?Met?Asp?Ala?Val?Ala?Glu?Ser?Asn?Val?Phe?Lys?Ser?Asn?Ile

225 230 235 240

Val?Gly?Phe?Ala?Leu?Ser?Arg?Ser?Pro?Ala?Lys?Asp?Gly?Thr?Val?Ser

245 250 255

Phe?Gly?Thr?Thr?Asp?Lys?Asp?Lys?Tyr?Thr?Gly?Asp?Ile?Thr?Tyr?Thr

260 265 270

Asp?Thr?Val?Gly?Ser?Asp?Ser?Tyr?Trp?Arg?Ile?Pro?Val?Asp?Asp?Val

275 280 285

Tyr?Val?Gly?Gly?Thr?Ser?Cys?Asp?Phe?Ser?Asn?Lys?Ser?Ala?Ile?Ile

290 295 300

Asp?Thr?Gly?Thr?Ser?Tyr?Ala?Met?Leu?Pro?Ser?Ser?Asp?Ser?Lys?Thr

305 310 315 320

Leu?His?Ser?Leu?Ile?Pro?Gly?Ala?Lys?Ser?Ser?Gly?Ser?Tyr?His?Ile

325 330 335

Ile?Pro?Cys?Asn?Thr?Thr?Thr?Lys?Leu?Gln?Val?Ala?Phe?Ser?Gly?Val

340 345 350

Asn?Tyr?Thr?Ile?Ser?Pro?Lys?Asp?Tyr?Val?Gly?Ala?Thr?Ser?Gly?Ser

355 360 365

Gly?Cys?Val?Ser?Asn?Ile?Ile?Ser?Tyr?Asp?Leu?Phe?Gly?Asp?Asp?Ile

370 375 380

Trp?Leu?Leu?Gly?Asp?Thr?Phe?Leu?Lys?Asn?Val?Tyr?Ala?Val?Phe?Asp

385 390 395 400

Tyr?Asp?Glu?Leu?Arg?Val?Gly?Phe?Ala?Glu?Arg?Ser?Ser?Asn?Thr?Thr

405 410 415

Ser?Ala?Ser?Asn?Ser?Thr?Ser?Ser?Gly?Thr?Ser?Ser?Thr?Ser?Gly?Ser

420 425 430

Thr?Thr?Thr?Gly?Ser?Ser?Thr?Thr?Thr?Thr?Ser?Ser?Ala?Ser?Ser?Ser

435 440 445

Ser?Ser?Ser?Asp?Ala?Glu?Ser?Gly?Ser?Ser?Met?Thr?Ile?Pro?Ala?Pro

450 455 460

Gln?Tyr?Phe?Phe?Ser?Ala?Leu?Ala?Ile?Ala?Ser?Phe?Met?Leu?Trp?Leu

465 470 475 480

<210>116

<211>1099

<212>PRT

＜213〉aspergillus niger

<400>116

Met?Leu?Arg?Gly?Leu?Arg?Asp?Val?Val?Leu?Leu?Gln?Phe?Ala?Ile?Pro

1 5 10 15

Leu?Phe?Leu?Leu?Leu?His?Phe?Arg?Leu?Ser?Leu?Arg?Gly?Val?Ile?Thr

20 25 30

Gly?Phe?Gly?Ser?Lys?Ser?His?Phe?Gln?Arg?Pro?Leu?Ser?Lys?Met?Ser

35 40 45

Ser?Thr?Gln?Lys?Ser?His?Phe?Lys?Leu?Leu?Gln?Lys?Phe?Lys?Pro?Glu

50 55 60

Tyr?Ser?Pro?Ser?Glu?Phe?Ala?Gln?Tyr?Glu?Ser?Glu?Arg?Thr?Gly?Met

65 70 75 80

Arg?Val?Val?Val?Ile?Asp?Gln?Lys?Gly?Pro?Lys?Val?Thr?Gly?Tyr?Phe

85 90 95

Val?Leu?Ala?Thr?Glu?Ile?Leu?Asp?Asp?Ser?Gly?Ala?Pro?His?Thr?Leu

100 105 110

Glu?His?Leu?Cys?Phe?Met?Gly?Ser?Arg?Asn?Tyr?Arg?Tyr?Lys?Gly?Phe

115 120 125

Leu?Asp?Lys?Leu?Ala?Thr?Arg?Val?Tyr?Ser?Ser?Thr?Asn?Ala?Trp?Thr

130 135 140

Ala?Thr?Asp?His?Thr?Ala?Tyr?Thr?Leu?Asp?Thr?Ala?Gly?Trp?Glu?Gly

145 150 155 160

Phe?Ala?Gln?Ile?Leu?Pro?Val?Tyr?Leu?Glu?His?Val?Ile?Ala?Pro?Thr

165 170 175

Leu?Thr?Asp?Glu?Gly?Cys?Tyr?Thr?Glu?Val?His?His?Ile?Asp?Gly?Ala

180 185 190

Gly?Asp?Asp?Ala?Gly?Val?Val?Tyr?Ser?Glu?Met?Gln?Gly?Val?Gln?Asn

195 200 205

Asn?Ser?Ala?Glu?Leu?Ile?Asp?Leu?Thr?Ala?Arg?Arg?Leu?Thr?Tyr?Pro

210 215 220

His?Gly?Val?Gly?Phe?Arg?Tyr?Glu?Thr?Gly?Gly?Met?Met?Glu?Gln?Leu

225 230 235 240

Arg?Val?Leu?Thr?Ala?Asp?Arg?Ile?Arg?Ala?Phe?His?Arg?Glu?Met?Tyr

245 250 255

Gln?Pro?Lys?Asn?Leu?Cys?Leu?Ile?Ile?Thr?Gly?Glu?Val?Asp?His?Gln

260 265 270

Asn?Met?Leu?Glu?Thr?Leu?Asp?Lys?Phe?Glu?Asp?Thr?Ile?Leu?Asp?Val

275 280 285

Ile?Pro?Ser?Pro?Asp?Ser?Pro?Phe?Lys?Arg?Pro?Trp?Val?Asp?Ser?Lys

290 295 300

Gln?Ala?Pro?Pro?Leu?Glu?Lys?Ser?Ile?Val?Gln?Thr?Val?Glu?Phe?Pro

305 310 315 320

Glu?Glu?Asp?Glu?Ser?Phe?Gly?Glu?Ile?Glu?Ile?Arg?Phe?Leu?Gly?Pro

325 330 335

Asp?Cys?Thr?Asp?Pro?Val?Gln?Thr?Gly?Ala?Val?Asn?Val?Ala?Leu?Leu

340 345 350

Tyr?Leu?Ala?Gly?Ser?Ser?Ala?Ser?Leu?Leu?Asp?Asn?Ile?Leu?Val?Glu

355 360 365

Lys?Glu?Gln?Leu?Ala?Ser?Ala?Val?Tyr?Tyr?Ala?Thr?Glu?Asp?His?Pro

370 375 380

Ser?Ile?Glu?Ile?Arg?Phe?Thr?Leu?Thr?Ser?Val?Glu?Thr?Glu?Lys?Leu

385 390 395 400

Ala?Lys?Val?Glu?Gln?Arg?Phe?Phe?Glu?Val?Leu?Lys?Asp?Ala?Met?Glu

405 410 415

Lys?Asp?Leu?Asp?Met?Arg?Tyr?Ile?Lys?Glu?Cys?Ile?Asp?Arg?Gln?Arg

420 425 430

Arg?Thr?Trp?Lys?Phe?Ser?Thr?Glu?Ser?Ser?Ala?Ser?Ser?Phe?Ala?Glu

435 440 445

Tyr?Val?Ile?Ser?Asp?Phe?Leu?Phe?Gly?Lys?Arg?Asp?Gly?Ser?Thr?Met

450 455 460

Leu?Asp?Val?Ala?Thr?Leu?Gln?Glu?Tyr?Asp?Val?Leu?Glu?Lys?Trp?Ser

465 470 475 480

Glu?Glu?Gln?Trp?Arg?Ser?Phe?Ile?Lys?Thr?Trp?Ile?Ser?Asp?Ala?Asn

485 490 495

His?Val?Thr?Ile?Leu?Gly?Val?Pro?Ser?Val?Lys?Met?Ser?Asp?Thr?Leu

500 505 510

Lys?Lys?Glu?Glu?Glu?Ala?Arg?Val?Ala?Glu?Gln?Lys?Lys?Arg?Leu?Gly

515 520 525

Asp?Glu?Gly?Leu?Lys?Lys?Leu?Ala?Asp?Lys?Leu?Glu?Lys?Ala?Lys?Ala

530 535 540

Glu?Asn?Asp?Lys?Glu?Ile?Pro?Lys?Glu?Met?Leu?Glu?Arg?Phe?Gln?Ile

545 550 555 560

Pro?Gly?Ile?Glu?Ser?Ile?His?Phe?Val?Asp?Thr?Thr?Thr?Ala?Arg?Ser

565 570 575

Gly?Ala?Ala?Leu?Asp?Ala?Gly?Arg?Pro?Ser?His?Lys?Ala?Gln?Lys?Leu

580 585 590

Val?Asp?Ala?Asp?Gly?Ser?Asp?Leu?Pro?Leu?Phe?Ile?His?Phe?Glu?His

595 600 605

Ile?Pro?Ser?Ser?Phe?Val?Gln?Leu?Ser?Leu?Leu?Ile?Ser?Ala?Gln?Ala

610 615 620

Val?Pro?Val?Gln?Leu?Arg?Pro?Leu?Leu?Ser?Val?Tyr?Thr?Glu?Ala?Phe

625 630 635 640

Phe?Asn?Leu?Pro?Val?Asn?Arg?Asn?Gly?Glu?Thr?Ile?Asn?Phe?Glu?Gln

645 650 655

Val?Val?Val?Glu?Leu?Glu?Arg?Asp?Thr?Val?Gly?Tyr?Ser?Met?Glu?Gly

660 665 670

Ala?Arg?Ser?Leu?Gly?Asn?Ser?Glu?Met?Leu?Arg?Ile?Ser?Phe?Gln?Val

675 680 685

Glu?Leu?Glu?Lys?Tyr?His?Thr?Ala?Ile?Ala?Trp?Ile?Gln?Glu?Leu?Ser

690 695 700

Trp?Asn?Ser?Ile?Phe?Asp?Val?Glu?Arg?Leu?Arg?Ala?Ile?Thr?Ser?Arg

705 710 715 720

Leu?Leu?Ser?Asp?Val?Pro?Asp?Ser?Lys?Arg?Ser?Gly?Asp?Asp?Met?Leu

725 730 735

Ala?Ala?Val?His?Val?Met?Val?His?Tyr?Ala?Ala?Glu?Ser?Ile?Val?Arg

740 745 750

Ala?Arg?Ser?Thr?Leu?Val?Lys?Ala?Arg?Tyr?Leu?Lys?Arg?Ile?Lys?Lys

755 760 765

Gln?Leu?Ala?Glu?Glu?Pro?Lys?Ser?Val?Val?Ala?Arg?Met?Glu?Glu?Ile

770 775 780

Arg?Asp?Ala?Leu?Phe?Arg?Phe?Glu?Asn?Met?Arg?Val?Leu?Val?Ile?Ala

785 790 795 800

Asp?Leu?Glu?Lys?L Gln?Asn?Pro?Val?Ser?Ala?Trp?Lys?Pro?Phe?Ala

805 810 815

Glu?Arg?Leu?Gly?Ala?Gly?Ala?Pro?Leu?Gln?Pro?Ile?Thr?Thr?Arg?Arg

820 825 830

Pro?Leu?Leu?Ser?Glu?Ala?Gly?Gln?Lys?Leu?Gly?Gly?Lys?Ser?Tyr?Val

835 840 845

Val?Pro?Met?Pro?Thr?Ile?Asp?Ser?Ser?Phe?Ala?Tyr?Ala?Thr?Ala?Arg

850 855 860

Gly?Leu?Asp?Ser?Tyr?Asp?Asp?Pro?Arg?Leu?Pro?Ala?Leu?Met?Val?Ala

865 870 875 880

Ile?Ala?Tyr?Met?Asn?Ala?Val?Glu?Gly?Pro?Leu?Trp?Val?Ala?Val?Arg

885 890 895

Gly?Lys?Gly?Leu?Ala?Tyr?Gly?Thr?Asn?Phe?Ala?Tyr?Asn?Ile?Asp?Thr

900 905 910

Gly?Phe?Val?Asn?Phe?Asp?Val?Tyr?Arg?Ser?Pro?Asn?Ala?His?Lys?Ala

915 920 925

Phe?Asp?Ser?Ser?Lys?Gln?Ile?Val?Glu?Asp?His?Leu?Ser?Gly?Ala?Met

930 935 940

Pro?Phe?Asp?Pro?Leu?Met?Leu?Glu?Gly?Ser?Ile?Ser?Ser?Ile?Val?Val

945 950 955 960

Ser?Phe?Ala?Asn?Glu?Gln?Ser?Thr?Ile?Gly?Ser?Ala?Ala?Ser?Gly?Ser

965 970 975

Phe?Ile?Arg?Gln?Val?Ile?Arg?Arg?Leu?Pro?Ser?Asp?Tyr?Lys?Glu?Arg

980 985 990

Val?Leu?Lys?Gln?Val?Arg?Ala?Thr?Ser?Val?Asp?Asp?Val?Lys?Gly?Ala

995 1000 1005

Leu?Lys Asp?Ile?Ile?Leu?Pro Leu?Phe?Asn?Pro?Ser Thr?Ala?Asn

1010 1015 1020

Ile?Val Val?Thr?Cys?Ala?Thr Val?Leu?Glu?Glu?Thr Ile?Lys?Glu

1025 1030 1035

Gly?Leu Gln?Ala?Ser?Gly?Phe Thr?Pro?Ala?Val?Gln Pro?Leu?Lys

1040 1045 1050

Glu?Phe Glu?Asp?Asp?Tyr?Gly Leu?Lys?Val?Gly?Asp Asp?Glu?Asp

1055 1060 1065

Glu?Glu Ser?Asp?Asp?Asp?Asp Asp?Glu?Tyr?Glu?Thr Gly?Ser?Glu

1070 1075 1080

Asp?Glu Asp?Asp?Ser?Asp?Glu Asp?Met?Glu?Asp?Asp Glu?Asp?Asp

1085 1090 1095

Glu

<210>117

<211>726

<212>PRT

＜213〉aspergillus niger

<400>117

Met?Gly?Ala?Leu?Gln?Trp?Leu?Ser?Ile?Thr?Ala?Ala?Ala?Ala?Ser?Ala

1 5 10 15

Val?Ser?Ala?Leu?Thr?Pro?Glu?Gln?Met?Ile?Gly?Ala?Pro?Arg?Arg?Thr

20 25 30

Glu?Val?Ile?Pro?Asn?Pro?Ser?Gly?Asp?Thr?Gly?Leu?Phe?Ser?Thr?Ser

35 40 45

Gln?Trp?Ser?Phe?Asp?Thr?His?Ser?Glu?Ser?Thr?Trp?Trp?Ser?Leu?Ile

50 55 60

Asp?Leu?Gln?Ser?Gly?Lys?Thr?Thr?Thr?Leu?Thr?Asp?Asp?Ser?Asp?Ile

65 70 75 80

Glu?Glu?Ile?Ile?Trp?Leu?Gly?Ser?Asp?Asn?Ser?Thr?Leu?Leu?Tyr?Ile

85 90 95

Asn?Ser?Thr?Asn?Ala?Gln?Val?Pro?Gly?Gly?Val?Glu?Leu?Trp?Ile?Ala

100 105 110

Asp?Ser?Ser?Asp?Phe?Ala?Asn?Ala?Tyr?Lys?Ala?Ala?Ser?Leu?Ser?Ala

115 120 125

Gly?Phe?Leu?Gly?Ile?Lys?Ser?Thr?Val?Thr?Asp?Ser?Gly?Asp?Val?His

130 135 140

Phe?Ile?Leu?Arg?Gly?Lys?Ser?Tyr?Pro?Asn?Gly?Thr?Ala?Tyr?Asn?Asp

145 150 155 160

Gln?Leu?Ala?Glu?Thr?Tyr?Pro?Ser?Thr?Ala?Arg?Ile?Tyr?Asp?Ser?Ile

165 170 175

Phe?Val?Arg?His?Trp?Asp?Thr?Tyr?Leu?Thr?Thr?Ala?Ser?His?Ala?Val

180 185 190

Phe?Ser?Gly?Thr?Leu?Gln?Ser?Ser?Thr?Ser?Asp?Asp?Gly?Asn?Val?Gln

195 200 205

Tyr?Thr?Ser?Ser?Gly?Gly?Leu?Thr?Asn?Leu?Val?Asn?Pro?Val?Lys?Gly

210 215 220

Ala?Glu?Ser?Pro?Phe?Pro?Pro?Phe?Gly?Gly?Asn?Asp?Asp?Tyr?Asp?Leu

225 230 235 240

Ser?Pro?Asp?Gly?Lys?Trp?Val?Thr?Phe?Lys?Ser?Lys?Ala?Pro?Glu?Leu

245 250 255

Pro?Leu?Ala?Asn?Asn?Thr?Ala?Ala?Tyr?Val?Tyr?Leu?Val?Pro?His?Asp

260 265 270

Gly?Ser?Ala?Thr?Ala?Phe?Ala?Val?Asn?Gly?Pro?Asp?Ser?Pro?Ala?Thr

275 280 285

Pro?Glu?Gly?Val?Glu?Gly?Glu?Ser?Asn?Asn?Pro?Val?Phe?Ser?Pro?Asp

290 295 300

Ser?Asp?Lys?Ile?Ala?Tyr?Phe?Gln?Met?Ala?Thr?Asn?Thr?Tyr?Glu?Ser

305 310 315 320

Asp?Arg?Asn?Val?Leu?Tyr?Val?Tyr?Ser?Ile?Ala?Asp?Asp?Thr?Ile?Thr

325 330 335

Pro?Leu?Ala?Lys?Asp?Trp?Asp?Arg?Ser?Pro?Ser?Ser?Val?Thr?Trp?Val

340 345 350

Asp?Gly?Asp?Asn?Leu?Val?Val?Ala?Ser?Gln?Asp?Leu?Gly?Arg?Thr?Arg

355 360 365

Leu?Phe?Ala?Ile?Pro?Gly?Asp?Ala?Gly?Asp?Asp?Phe?Lys?Pro?Thr?Asn

370 375 380

Phe?Thr?Asp?Gly?Gly?Ser?Val?Ser?Ala?Gln?Tyr?Val?Leu?Ser?Asn?Ser

385 390 395 400

Thr?Leu?Leu?Val?Thr?Ser?Ser?Ala?Phe?Trp?Thr?Ser?Trp?Ser?Val?Tyr

405 410 415

Thr?Ala?Ser?Pro?Asp?Glu?Gly?Val?Ile?Asn?Thr?Leu?Ala?Ser?Ala?Asn

420 425 430

Glu?Ile?Asp?Pro?Glu?Leu?Ser?Gly?Leu?Ser?Ser?Ser?Asp?Phe?Glu?Glu

435 440 445

Phe?Tyr?Phe?Asp?Gly?Asn?Trp?Thr?Thr?Leu?Gln?Gly?Trp?Ile?Thr?Tyr

450 455 460

Pro?Gln?Asp?Phe?Asp?Ser?Ser?Lys?Lys?Tyr?Pro?Leu?Ala?Phe?Leu?Ile

465 470 475 480

His?Gly?Gly?Pro?Glu?Asp?Ala?Trp?Ala?Asp?Glu?Trp?Asn?Leu?Lys?Trp

485 490 495

His?Ser?Lys?Val?Phe?Ala?Asp?Gln?Gly?Tyr?Val?Val?Val?Gln?Pro?Asn

500 505 510

Pro?Thr?Gly?Ser?Thr?Gly?Phe?Gly?Gln?Gln?Leu?Thr?Asp?Ala?Ile?Gln

515 520 525

Leu?Asn?Trp?Thr?Gly?Ala?Ala?Tyr?Asp?Asp?Leu?Thr?Lys?Ala?Trp?Gln

530 535 540

Tyr?Val?His?Asp?Thr?Tyr?Asp?Phe?Ile?Asp?Thr?Asp?Asn?Gly?Val?Ala

545 550 555 560

Ala?Gly?Pro?Ser?Phe?Gly?Ala?Phe?Met?Ile?Thr?Trp?Ile?Gln?Gly?Asp

565 570 575

Asp?Phe?Gly?Arg?Lys?Phe?Lys?Ala?Leu?Val?Ser?His?Asp?Gly?Pro?Phe

580 585 590

Ile?Gly?Asp?Ala?Trp?Val?Glu?Thr?Asp?Glu?Leu?Trp?Phe?Val?Glu?His

595 600 605

Glu?Phe?Asn?Gly?Thr?Phe?Trp?Gln?Ala?Arg?Asp?Ala?Phe?His?Asn?Thr

610 615 620

Asp?Pro?Ser?Gly?Pro?Ser?Arg?Val?Leu?Ala?Tyr?Ser?Thr?Pro?Gln?Leu

625 630 635 640

Val?Ile?His?Ser?Asp?Lys?Asp?Tyr?Arg?Ile?Pro?Val?Ala?Asn?Gly?Ile

645 650 655

Gly?Leu?Phe?Asn?Thr?Leu?Gln?Glu?Arg?Gly?Val?Pro?Ser?Arg?Phe?Leu

660 665 670

Asn?Phe?Pro?Asp?Glu?Asp?His?Trp?Val?Thr?Gly?Gln?Glu?Asn?Ser?Leu

675 680 685

Val?Trp?Tyr?Gln?Gln?Val?Leu?Gly?Trp?Ile?Asn?Arg?Tyr?Ser?Gly?Val

690 695 700

Gly?Gly?Ser?Asn?Pro?Asp?Ala?Ile?Ala?Leu?Glu?Asp?Thr?Val?Asn?Pro

705 710 715 720

Val?Val?Asp?Leu?Asn?Pro

725

<210>118

<211>564

<212>PRT

＜213〉aspergillus niger

<400>118

Met?Thr?Arg?Gln?Thr?Ser?Leu?Val?Pro?Arg?Leu?Leu?Thr?Leu?Ala?Ser

1 5 10 15

Leu?Ala?Ala?Leu?Ser?Gln?Ala?Glu?Leu?Gly?Lys?Ile?Gln?Trp?Lys?Gly

20 25 30

Ser?Cys?Asn?Leu?Thr?Thr?Tyr?Pro?Ala?Leu?Ile?Cys?Gly?Thr?Leu?Asp

35 40 45

Val?Pro?Tyr?Asp?Tyr?Thr?Glu?Ser?Asn?Ser?Ser?Lys?Thr?Leu?Thr?Leu

50 55 60

Asp?Ile?Ala?Lys?Trp?Pro?Ala?Thr?Lys?Lys?Pro?Val?Ser?Glu?Pro?Ile

65 70 75 80

Ile?Phe?Asn?Phe?Gly?Gly?Pro?Gly?Val?Asn?Ser?Phe?Glu?Gly?Leu?Gly

85 90 95

Leu?Tyr?Gly?Glu?Glu?Phe?Gln?Ala?Ile?Leu?Gly?Gly?His?Asn?Asp?Leu

100 105 110

Ile?Ala?Phe?Asn?Asn?Arg?Gly?Val?Gly?Asn?Thr?Ile?Pro?Phe?Ser?Cys

115 120 125

Tyr?Ser?Asp?Asp?Ala?Thr?Arg?Glu?Leu?Val?Ala?Leu?Gln?Ala?Pro?Asn

130 135 140

Asp?Gly?Arg?Ala?Ser?Ser?Thr?Ala?Leu?Gly?Glu?Ile?Trp?Ala?Gln?Asn

145 150 155 160

Ala?Asn?Ile?Ala?Gln?Ala?Cys?Tyr?Ala?Thr?Asn?Asn?Gln?Thr?Gly?Ser

165 170 175

Leu?Ile?Gly?Thr?Ser?Phe?Ala?Ala?Arg?Asp?Ile?Met?Gln?Val?Ala?Asp

180 185 190

Ala?Leu?Ser?Gly?Lys?Asp?Ser?Leu?Val?Asn?Tyr?Trp?Gly?Phe?Ser?Tyr

195 200 205

Gly?Thr?Thr?Ile?Gly?Ala?Val?Leu?Ala?Ala?Met?Phe?Pro?Asp?Arg?Met

210 215 220

Gly?Asn?Val?Ala?Leu?Asp?Gly?Val?Asp?Asn?Pro?Arg?Glu?Ala?Leu?Tyr

225 230 235 240

Gly?Tyr?Asn?Ala?Gln?Ala?Val?Val?Asp?Val?Asp?Lys?Val?Phe?Glu?Gly

245 250 255

Phe?Cys?Thr?Gly?Cys?Met?Ala?Ala?Pro?Asp?Leu?Cys?Pro?Ile?Ala?Lys

260 265 270

Glu?Tyr?Thr?Ser?Ala?Ala?Asn?Leu?Glu?Ala?Ala?Ile?Tyr?Leu?Met?Leu

275 280 285

Glu?Asn?Leu?Lys?Tyr?Asn?Pro?Ile?Ala?Ile?Pro?Glu?Thr?Gly?Gly?Ile

290 295 300

Val?Thr?Trp?Ser?Asp?Val?Lys?Ser?Thr?Ile?Phe?Glu?Ala?Met?Tyr?Leu

305 310 315 320

Pro?Ser?Ser?Trp?Pro?Leu?Thr?Ser?Glu?Leu?Leu?Tyr?Tyr?Val?Gln?Thr

325 330 335

Arg?Asn?Thr?Thr?Ile?Leu?Gly?Asn?Ser?Glu?Val?Tyr?Asp?Thr?Ile?Lys

340 345 350

Ser?Tyr?Gly?Gln?Ser?Ala?Ser?Leu?Thr?Ser?Ala?Ser?Asp?Glu?Val?Gly

355 360 365

Thr?Ala?Ile?Thr?Cys?Ser?Asp?Lys?His?Arg?Ser?Ala?Thr?Ile?Lys?Glu

370 375 380

Val?Leu?Pro?Tyr?Val?Lys?Ala?Arg?Gln?Ala?Leu?Thr?Lys?Ile?Gly?Ser

385 390 395 400

Asp?Gly?Ser?Asp?Gly?Asp?Met?Arg?Cys?Ala?Gln?Trp?Asn?Pro?Lys?Met

405 410 415

Phe?Ala?Lys?Glu?Arg?Tyr?Ser?Gly?Asp?Phe?Glu?Val?Lys?Thr?Ala?Asn

420 425 430

Pro?Val?Leu?Ile?Leu?Ser?Asn?Thr?Tyr?Asp?Pro?Ala?Thr?Pro?Leu?Pro

435 440 445

Ala?Ala?Lys?Asn?Leu?Thr?Glu?Thr?Phe?Glu?Gly?Ser?Val?Leu?Leu?Glu

450 455 460

Gln?Asn?Gly?Tyr?Gly?His?Thr?Thr?Leu?Ser?Met?Pro?Ser?Leu?Cys?Thr

465 470 475 480

Ala?Lys?Ala?Val?Arg?Ala?Tyr?Phe?Thr?Asn?Gly?Thr?Leu?Pro?Ala?Asp

485 490 495

Gly?Thr?Ile?Cys?Gln?Val?Asp?Val?Pro?Leu?Phe?Thr?Asn?Leu?Thr?Tyr

500 505 510

Lys?Asp?Val?Trp?Pro?Lys?Ser?Phe?Gln?Arg?Ser?Val?Glu?Ser?Arg?Asp

515 520 525

Asp?Ala?Thr?Ile?Leu?Lys?Ala?Leu?Met?Ser?Val?Arg?Asp?Lys?Met?Ser

530 535 540

Arg?Arg?Arg?Met?Cys?Ile?Tyr?Leu?Tyr?Thr?Asn?Ser?Ala?Ser?Trp?Arg

545 550 555 560

Pro?Glu?Leu?Pro

<210>119

<211>526

<212>PRT

＜213〉aspergillus niger

<400>119

Met?Tyr?Tyr?Ser?Leu?Trp?Val?Ala?Ala?Leu?Val?Ala?Ala?Leu?Pro?Val

1 5 10 15

Ser?Arg?Ala?Gln?Phe?Val?Ala?Pro?Pro?Thr?Asp?Leu?Ile?Pro?Thr?Lys

20 25 30

Gly?Tyr?Leu?Asp?Ile?Pro?Val?Arg?Tyr?Lys?Gln?Val?Pro?Thr?Gly?Ile

35 40 45

Cys?Glu?Thr?Asp?Pro?Ser?Val?Lys?Ser?Phe?Ser?Gly?Tyr?Val?Asp?Val

50 55 60

Ala?Glu?His?Glu?His?Ile?Phe?Phe?Trp?Phe?Phe?Glu?Ala?Arg?Asn?Gln

65 70 75 80

Asp?Pro?Thr?Glu?Ala?Pro?Leu?Thr?Val?Trp?Ile?Asn?Gly?Gly?Met?Ser

85 90 95

Asp?Pro?Gly?Pro?Gly?Ser?Ser?Ser?Met?Ile?Gly?Leu?Phe?Gln?Glu?His

100 105 110

Gly?Pro?Cys?Gly?Ile?Asp?Ala?Asn?Gly?Ser?Val?Tyr?Asn?Asn?Pro?Tyr

115 120 125

Ser?Trp?Asn?Asn?Ala?Ser?Asn?Met?Leu?Tyr?Ile?Asp?Gln?Pro?Val?Gln

130 135 140

Thr?Gly?Phe?Ser?Tyr?Ser?Ile?Pro?Val?Pro?Gly?Tyr?Val?Asp?Ser?Ser

145 150 155 160

Thr?Asp?Asn?Gly?Phe?Met?Gly?Ala?Phe?Pro?Gln?Tyr?Ser?Arg?Glu?Thr

165 170 175

Phe?His?Phe?Thr?Thr?Glu?Ser?Tyr?Gly?Gly?His?Tyr?Gly?Pro?Val?Phe

180 185 190

Asn?Glu?Tyr?Ile?Glu?Glu?Gln?Asn?Ala?His?Leu?Gln?Pro?Gly?Ala?Lys

195 200 205

Lys?Ile?Gln?Leu?Gly?Ser?Val?Met?Ile?Gly?Asn?Gly?Trp?Tyr?Asp?Pro

210 215 220

Ile?Ile?Gln?Tyr?Gln?Ala?Tyr?Tyr?Asn?Phe?Thr?Val?Tyr?Pro?Gly?Asn

225 230 235 240

Thr?Tyr?Asp?Tyr?Leu?Pro?Phe?Asn?Lys?Ser?Ile?Ser?Ser?Leu?Met?Tyr

245 250 255

Asn?Asn?Leu?Tyr?Gly?Pro?Gly?Asn?Cys?Leu?Asp?Gln?Leu?Tyr?Asp?Cys

260 265 270

Ala?Ala?Arg?Gly?Ile?Asp?Glu?Ile?Cys?Ser?Thr?Ala?Asp?Asp?Phe?Cys

275 280 285

Ala?Asn?Glu?Val?Glu?Asn?Val?Tyr?Asp?Ile?Tyr?Ser?Gly?Arg?Asp?Glu

290 295 300

Tyr?Asp?Phe?Arg?Glu?Leu?Thr?Pro?Asp?Pro?Phe?Pro?Tyr?Glu?Phe?Tyr

305 310 315 320

Val?Asp?Tyr?Leu?Asn?Lys?Ala?Ser?Val?Gln?Ala?Ala?Ile?Gly?Ala?Tyr

325 330 335

Ile?Asn?Tyr?Thr?Glu?Ser?Asn?Asn?Ala?Val?Gly?Leu?Ala?Phe?Ser?Ser

340 345 350

Thr?Gly?Asp?Asp?Gly?Arg?Leu?Met?Asn?Thr?Ile?Gln?Asp?Val?Gly?Lys

355 360 365

Leu?Leu?Lys?Gln?Gly?Val?Thr?Val?Val?Met?Tyr?Ala?Gly?Asp?Ala?Asp

370 375 380

Tyr?Asn?Cys?Asn?Trp?Leu?Gly?Gly?Glu?Ala?Val?Ser?Leu?Gln?Val?Lys

385 390 395 400

Ala?Ala?Asn?Phe?Ser?Ser?Ala?Gly?Tyr?Thr?Asn?Ile?Val?Thr?Ser?Asp

405 410 415

Gly?Val?Thr?His?Gly?Gln?Val?Arg?Gln?Ala?Gly?Gln?Phe?Ala?Phe?Val

420 425 430

Arg?Val?Tyr?Glu?Ser?Gly?His?Glu?Val?Pro?Phe?Tyr?Gln?Pro?Leu?Leu

435 440 445

Ala?Leu?Glu?Met?Phe?Glu?Arg?Val?Ile?Gly?Gly?Lys?Asp?Val?Ala?Thr

450 455 460

Gly?Lys?Ile?Pro?Ile?Ser?Ser?Ser?Leu?Gln?Thr?Val?Gly?Thr?Pro?Lys

465 470 475 480

Ser?Tyr?Tyr?Arg?Glu?Gly?Asn?Ser?Thr?Ile?Gln?Trp?Glu?Val?Leu?Asp

485 490 495

Ser?Leu?Ala?Thr?Tyr?Asn?Thr?Thr?Thr?Asn?Ala?Pro?Asn?Pro?Val?Ser

500 505 510

Arg?Arg?Leu?Lys?Arg?Met?Gly?Pro?Ala?Leu?Arg?Phe?Gln?Met

515 520 525

<210>120

<211>1156

<212>PRT

＜213〉aspergillus niger

<400>120

Met?Ser?Cys?Val?Trp?Leu?His?Ile?His?Lys?Arg?Ser?Leu?Leu?Ser?Val

1 5 10 15

Ala?Thr?Asn?Asn?Ser?Val?Ala?Arg?Ala?Ala?Ala?Ser?Thr?Ser?Ala?Ala

20 25 30

Pro?Pro?Pro?Pro?Ser?Ser?Pro?Pro?Pro?Gly?Ser?Asn?Thr?Tyr?Ser?Pro

35 40 45

Leu?Tyr?Arg?Pro?Ile?Thr?Asn?Pro?Ile?Gly?Phe?Thr?Leu?Ser?Pro?Ala

50 55 60

Arg?Ser?Leu?Val?Ser?Arg?Asn?Pro?Lys?Phe?Pro?Ala?Tyr?Arg?Arg?Ser

65 70 75 80

Ser?Arg?His?Phe?Ser?Leu?Cys?Pro?Ala?Ala?Ala?Thr?Pro?Gly?Val?Thr

85 90 95

Thr?Ser?Ile?Cys?Pro?Gly?Gln?Ala?Pro?Val?Arg?Ser?Leu?Ser?Ser?Leu

100 105 110

Ile?Ile?His?Ser?Thr?Arg?Pro?Arg?Ala?Ile?Arg?Ile?Arg?Thr?Asp?Gln

115 120 125

Met?Asp?Leu?Asn?Gly?Asp?Ala?Gly?Ala?Lys?Arg?Lys?Arg?Ser?Ser?Ile

130 135 140

Thr?Thr?Pro?Ala?Glu?Arg?Pro?Val?Lys?His?Leu?Arg?Pro?Glu?Ser?Ser

145 150 155 160

Ala?Leu?Thr?Pro?Gly?Asp?Ser?Thr?Pro?Ala?Asn?Gly?Thr?Val?Tyr?Asp

165 170 175

Val?Glu?Asp?Asp?Glu?Asp?Ala?Ser?Arg?Leu?Leu?Pro?Val?Gly?Pro?Ala

180 185 190

Gln?Ala?Asp?Ser?Pro?Glu?Trp?Gln?Ala?Thr?Ile?Glu?Glu?Val?Val?Lys

195 200 205

Ser?Val?Val?Ser?Ile?His?Phe?Cys?Gln?Thr?Cys?Ser?Phe?Asp?Thr?Glu

210 215 220

Leu?Ser?Met?Ser?Ser?Gln?Ala?Thr?Gly?Phe?Val?Val?Asp?Ala?Glu?Asn

225 230 235 240

Gly?Tyr?Ile?Leu?Thr?Asn?Arg?His?Val?Val?Cys?Pro?Gly?Pro?Phe?Trp

245 250 255

Gly?Tyr?Cys?Ile?Phe?Asp?Asn?His?Glu?Glu?Cys?Asp?Val?Arg?Pro?Val

260 265 270

Tyr?Arg?Asp?Pro?Val?His?Asp?Phe?Gly?Ile?Leu?Lys?Phe?Asp?Pro?Lys

275 280 285

Ala?Ile?Arg?Tyr?Met?Lys?Leu?Arg?Glu?Leu?Lys?Leu?Gln?Pro?Asp?Ala

290 295 300

Ala?Lys?Val?Gly?Ser?Glu?Ile?Arg?Val?Val?Gly?Asn?Asp?Ala?Gly?Glu

305 310 315 320

Lys?Leu?Ser?Ile?Leu?Ser?Gly?Val?Ile?Ser?Arg?Leu?Asp?Arg?Asn?Ala

325 330 335

Pro?Glu?Tyr?Gly?Asp?Gly?Tyr?Ser?Asp?Phe?Asn?Thr?Asn?Tyr?Ile?Gln

340 345 350

Ala?Ala?Ala?Ala?Ala?Ser?Gly?Gly?Ser?Ser?Gly?Ser?Pro?Val?Val?Asn

355 360 365

Ile?Asp?Gly?His?Ala?Ile?Ala?Leu?Gln?Ala?Gly?Gly?Arg?Ala?Asp?Gly

370 375 380

Ala?Ala?Thr?Asp?Tyr?Phe?Leu?Pro?Leu?Asp?Arg?Pro?Leu?Arg?Ala?Leu

385 390 395 400

Glu?Cys?Ile?Arg?Arg?Gly?Glu?Pro?Val?Thr?Arg?Gly?Thr?Ile?Gln?Thr

405 410 415

Gln?Trp?Ile?Leu?Lys?Pro?Phe?Asp?Glu?Cys?Arg?Arg?Leu?Gly?Leu?Thr

420 425 430

Pro?Glu?Trp?Glu?Ala?Thr?Val?Arg?Lys?Ala?Ala?Pro?Thr?Glu?Thr?Ser

435 440 445

Met?Leu?Val?Ala?Glu?Ile?Ile?Leu?Pro?Glu?Gly?Pro?Ala?Asp?Gly?Lys

450 455 460

Leu?Glu?Glu?Gly?Asp?Val?Leu?Leu?Gln?Val?Asn?Gly?Val?Leu?Leu?Thr

465 470 475 480

Gln?Phe?Ile?Arg?Leu?Asp?Asp?Ile?Leu?Asp?Ser?Ser?Val?Gly?Gln?Thr

485 490 495

Val?Arg?Leu?Leu?Val?Gln?Arg?Gly?Gly?Gln?Asn?Val?Glu?Ile?Glu?Cys

500 505 510

Gln?Val?Gly?Asp?Leu?His?Ala?Ile?Thr?Pro?Asp?Arg?Phe?Val?Thr?Val

515 520 525

Ala?Gly?Gly?Thr?Phe?His?Asn?Leu?Ser?Tyr?Gln?Gln?Ser?Arg?Leu?Tyr

530 535 540

Ala?Ile?Ala?Thr?Arg?Gly?Val?Tyr?Val?Cys?Glu?Ala?Ala?Gly?Ser?Phe

545 550 555 560

Lys?Leu?Glu?Asn?Thr?Leu?Ser?Gly?Trp?Ile?Ile?Asp?Ser?Val?Asp?Lys

565 570 575

Arg?Pro?Thr?Arg?Asn?Leu?Asp?Glu?Phe?Val?Glu?Val?Met?Arg?Thr?Ile

580 585 590

Pro?Asp?Arg?Ser?Arg?Val?Val?Ile?Ser?Tyr?Arg?His?Ile?Arg?Asp?Leu

595 600 605

His?Thr?Arg?Gly?Thr?Ser?Ile?Val?Tyr?Ile?Asp?Arg?His?Trp?His?Pro

610 615 620

Lys?Met?Arg?Leu?Ala?Val?Arg?Asn?Asp?Asp?Thr?Gly?Leu?Trp?Asp?Phe

625 630 635 640

Ser?Asp?Leu?Ala?Asp?Pro?Ile?Pro?Ala?Leu?Pro?Pro?Val?Pro?Arg?Lys

645 650 655

Ala?Asp?Phe?Ile?Gln?Leu?Asp?Gly?Val?Ser?Gln?Pro?Ala?Ala?Ala?Asp

660 665 670

Ile?Val?Arg?Ser?Phe?Val?Arg?Val?Ser?Cys?Thr?Met?Pro?Leu?Lys?Leu

675 680 685

Asp?Gly?Tyr?Pro?Gln?Ala?Lys?Lys?Thr?Gly?Phe?Gly?Leu?Val?Val?Asp

690 695 700

Ala?Glu?Lys?Gly?Leu?Val?Val?Val?Ser?Arg?Ala?Ile?Val?Pro?Tyr?Asp

705 710 715 720

Leu?Cys?Asp?Ile?Asn?Val?Thr?Val?Ala?Asp?Ser?Ile?Ile?Val?Asn?Ala

725 730 735

Lys?Val?Val?Phe?Leu?His?Pro?Leu?Gln?Asn?Tyr?Ser?Ile?Ile?Gln?Tyr

740 745 750

Asp?Pro?Ser?Leu?Val?Gln?Ala?Pro?Val?Gln?Ser?Ala?Lys?Leu?Ala?Thr

755 760 765

Asp?Tyr?Ile?Lys?Gln?Gly?Gln?Asp?Thr?Ile?Phe?Val?Gly?Phe?Asn?Gln

770 775 780

Asn?Phe?Arg?Ile?Val?Val?Ala?Lys?Thr?Ala?Val?Thr?Asp?Ile?Thr?Thr

785 790 795 800

Val?Ser?Ile?Pro?Ala?Asn?Ala?Ser?Ala?Pro?Arg?Tyr?Arg?Ala?Ile?Asn

805 810 815

Leu?Asp?Ala?Ile?Thr?Val?Asp?Thr?Gly?Leu?Ser?Gly?Gln?Cys?Ser?Asn

820 825 830

Gly?Val?Leu?Ile?Gly?Glu?Asp?Gly?Val?Val?Gln?Ala?Leu?Trp?Leu?Asn

835 840 845

Tyr?Leu?Gly?Glu?Arg?Thr?Ser?Asn?Ser?His?Lys?Asp?Val?Glu?Tyr?His

850 855 860

Leu?Gly?Phe?Ala?Thr?Pro?Ser?Leu?Leu?Pro?Val?Leu?Ser?Lys?Val?Gln

865 870 875 880

Gln?Gly?Glu?Met?Pro?Glu?Leu?Arg?Ile?Leu?Asn?Met?Glu?Ser?Tyr?Val

885 890 895

Val?Gln?Met?Ser?Gln?Ala?Arg?Ile?Met?Gly?Val?Ser?Glu?Glu?Trp?Ile

900 905 910

Glu?Lys?Val?Thr?Gln?Ala?Asn?Pro?Ser?Arg?His?Gln?Leu?Phe?Met?Val

915 920 925

Arg?Lys?Val?Asp?Cys?Pro?Pro?Pro?Gly?Phe?Asn?Ser?Ala?Ala?Asp?Thr

930 935 940

Phe?Glu?Glu?Gly?Asp?Ile?Ile?Leu?Thr?Leu?Asp?Gly?Gln?Leu?Ile?Thr

945 950 955 960

Arg?Val?Ser?Glu?Leu?Asp?Ile?Met?Tyr?Glu?Lys?Asp?Thr?Leu?Glu?Ala

965 970 975

Leu?Ile?Val?Arg?Asn?Gly?Gln?Glu?Met?Arg?Ile?Gln?Val?Pro?Thr?Val

980 985 990

Pro?Thr?Glu?Asp?Leu?Glu?Thr?Asp?Arg?Ala?Val?Val?Phe?Cys?Gly?Ala

995 1000 1005

Val?Leu?Gln?Lys?Pro?His?His?Ala?Val?Arg?Gln?Gln?Ile?Ser?Lys

1010 1015 1020

Leu?His?Ser?Glu?Val?Tyr?Val?Ser?Ala?Arg?Ser?Arg?Gly?Ser?Pro

1025 1030 1035

Ser?Tyr?Gln?Tyr?Gly?Leu?Ala?Pro?Thr?Asn?Phe?Ile?Thr?Ala?Val

1040 1045 1050

Asn?Gly?Val?Pro?Thr?Pro?Asn?Leu?Asp?Arg?Phe?Ser?Glu?Glu?Val

1055 1060 1065

Ser?Lys?Ile?Pro?Asp?Asn?Thr?Tyr?Phe?Arg?Leu?Arg?Ala?Val?Thr

1070 1075 1080

Phe?Asp?Asn?Val?Pro?Trp?Val?Val?Thr?Val?Lys?Lys?Asn?Asp?His

1085 1090 1095

Tyr?Phe?Pro?Met?Ser?Glu?Tyr?Ile?Lys?Asp?Gln?Ser?Gln?Pro?Ser

1100 1105 1110

Gly?Trp?Arg?Thr?Val?Ser?His?Asp?Lys?Asp?Lys?Tyr?Lys?Asp?Gly

1115 1120 1125

Ile?Ala?Pro?Asp?Ala?Ala?Asn?Leu?Asn?Pro?Asp?Ala?Met?Asp?Glu

1130 1135 1140

Gly?Phe?Asp?Gly?Val?Ser?Asp?Ile?Glu?Pro?Asp?Leu?Glu

1145 1150 1155

<210>121

<211>536

<212>PRT

＜213〉aspergillus niger

<400>121

Met?Arg?Val?Leu?Pro?Ala?Ala?Met?Leu?Val?Gly?Ala?Ala?Thr?Ala?Ala

1 5 10 15

Val?Pro?Pro?Phe?Gln?Gln?Val?Leu?Gly?Gly?Asn?Gly?Ala?Lys?His?Gly

20 25 30

Ala?Asp?His?Ala?Ala?Glu?Val?Pro?Ala?Asp?His?Ser?Ala?Asp?Gly?Phe

35 40 45

Ser?Lys?Pro?Leu?His?Ala?Phe?Gln?Glu?Glu?Leu?Lys?Ser?Leu?Ser?Asp

50 55 60

Glu?Ala?Arg?Lys?Leu?Trp?Asp?Glu?Val?Ala?Ser?Phe?Phe?Pro?Glu?Ser

65 70 75 80

Met?Asp?Gln?Asn?Pro?Leu?Phe?Ser?Leu?Pro?Lys?Lys?His?Asn?Arg?Arg

85 90 95

Pro?Asp?Ser?His?Trp?Asp?His?Ile?Val?Asp?Gly?Lys?Leu?Glu?Ala?Tyr

100 105 110

Asp?Leu?Arg?Val?Lys?Lys?Thr?Asp?Pro?Gly?Ser?Leu?Gly?Ile?Asp?Pro

115 120 125

Gly?Val?Lys?Gln?Tyr?Thr?Gly?Tyr?Leu?Asp?Asp?Asn?Glu?Asn?Asp?Lys

130 135 140

His?Leu?Phe?Tyr?Trp?Phe?Phe?Glu?Ser?Arg?Asn?Asp?Pro?Glu?Asn?Asp

145 150 155 160

Pro?Val?Val?Leu?Trp?Leu?Asn?Gly?Gly?Pro?Gly?Cys?Ser?Ser?Leu?Thr

165 170 175

Gly?Leu?Phe?Met?Glu?Leu?Gly?Pro?Ser?Ser?Ile?Asn?Lys?Lys?Ile?Gln

180 185 190

Pro?Val?Tyr?Asn?Asp?Tyr?Ala?Trp?Asn?Ser?Asn?Ala?Ser?Val?Ile?Phe

195 200 205

Leu?Asp?Gln?Pro?Val?Asn?Val?Gly?Tyr?Ser?Tyr?Ser?Asn?Ser?Ala?Val

210 215 220

Ser?Asp?Thr?Val?Ala?Ala?Gly?Lys?Asp?Val?Tyr?Ala?Leu?Leu?Thr?Leu

225 230 235 240

Phe?Phe?Lys?Gln?Phe?Pro?Glu?Tyr?Ala?Lys?Gln?Asp?Phe?His?Ile?Ala

245 250 255

Gly?Glu?Ser?Tyr?Ala?Gly?His?Tyr?Ile?Pro?Val?Phe?Ala?Ser?Glu?Ile

260 265 270

Leu?Ser?His?Lys?Lys?Arg?Asn?Ile?Asn?Leu?Gln?Ser?Val?Leu?Ile?Gly

275 280 285

Asn?Gly?Leu?Thr?Asp?Gly?Tyr?Thr?Gln?Tyr?Glu?Tyr?Tyr?Arg?Pro?Met

290 295 300

Ala?Cys?Gly?Asp?Gly?Gly?Tyr?Pro?Ala?Val?Leu?Asp?Glu?Ser?Ser?Cys

305 310 315 320

Gln?Ser?Met?Asp?Asn?Ala?Leu?Pro?Arg?Cys?Gln?Ser?Met?Ile?Glu?Ser

325 330 335

Cys?Tyr?Ser?Ser?Glu?Ser?Ala?Trp?Val?Cys?Val?Pro?Ala?Ser?Ile?Tyr

340 345 350

Cys?Asn?Asn?Ala?Leu?Leu?Ala?Pro?Tyr?Gln?Arg?Thr?Gly?Gln?Asn?Val

355 360 365

Tyr?Asp?Val?Arg?Gly?Lys?Cys?Glu?Asp?Ser?Ser?Asn?Leu?Cys?Tyr?Ser

370 375 380

Ala?Met?Gly?Tyr?Val?Ser?Asp?Tyr?Leu?Asn?Lys?Pro?Glu?Val?Ile?Glu

385 390 395 400

Ala?Val?Gly?Ala?Glu?Val?Asn?Gly?Tyr?Asp?Ser?Cys?Asn?Phe?Asp?Ile

405 410 415

Asn?Arg?Asn?Phe?Leu?Phe?His?Gly?Asp?Trp?Met?Lys?Pro?Tyr?His?Arg

420 425 430

Leu?Val?Pro?Gly?Leu?Leu?Glu?Gln?Ile?Pro?Val?Leu?Ile?Tyr?Ala?Gly

435 440 445

Asp?Ala?Asp?Phe?Ile?Cys?Asn?Trp?Leu?Gly?Asn?Lys?Ala?Trp?Thr?Glu

450 455 460

Ala?Leu?Glu?Trp?Pro?Gly?Gln?Ala?Glu?Tyr?Ala?Ser?Ala?Glu?Leu?Glu

465 470 475 480

Asp?Leu?Val?Ile?Val?Asp?Asn?Glu?His?Thr?Gly?Lys?Lys?Ile?Gly?Gln

485 490 495

Val?Lys?Ser?His?Gly?Asn?Phe?Thr?Phe?Met?Arg?Leu?Tyr?Gly?Gly?Gly

500 505 510

His?Met?Val?Pro?Met?Asp?Gln?Pro?Glu?Ser?Ser?Leu?Glu?Phe?Phe?Asn

515 520 525

Arg?Trp?Leu?Gly?Gly?Glu?Trp?Phe

530 535

<210>122

<211>279

<212>PRT

＜213〉aspergillus niger

<400>122

Met?Lys?Phe?Thr?Asn?Tyr?Leu?Leu?Thr?Thr?Ala?Thr?Leu?Ala?Ser?Ser

1 5 10 15

Val?Leu?Ala?Ala?Pro?Ala?Pro?Arg?Thr?Gly?Leu?Glu?Asp?Arg?Leu?Arg

20 25 30

Ala?Arg?Ser?Leu?Gln?Arg?Gln?Ser?His?Pro?Leu?Ala?Pro?Ile?Pro?Leu

35 40 45

Asp?Thr?Ser?Thr?Lys?Glu?Asn?Ser?Arg?Leu?Leu?Glu?Ala?Asp?Glu?Asn

50 55 60

Thr?Thr?His?Val?Thr?Tyr?Ser?Ser?Asn?Trp?Ala?Gly?Ala?Val?Arg?Glu

65 70 75 80

Gln?Pro?Pro?Pro?Gln?Gly?Thr?Tyr?Ser?Ala?Val?Ser?Ala?Thr?Phe?Arg

85 90 95

Val?Pro?Glu?Pro?Thr?Ala?Gln?Gly?Gly?Ser?Gly?Thr?Gln?Ala?Gly?Ser

100 105 110

Ala?Trp?Val?Gly?Ile?Asp?Gly?Asp?Thr?Tyr?Ser?Asn?Ala?Ile?Leu?Gln

115 120 125

Thr?Gly?Val?Asp?Phe?Tyr?Val?Glu?Asn?Gly?Gln?Thr?Tyr?Asn?Asp?Ala

130 135 140

Trp?Tyr?Glu?Trp?Tyr?Pro?Asp?Tyr?Ala?Tyr?Asp?Phe?Asp?Leu?Asp?Val

145 150 155 160

Ser?Thr?Gly?Asp?Thr?Ile?Val?Ala?Lys?Val?Glu?Ala?Ile?Ser?Pro?Ser

165 170 175

Gln?Gly?Val?Ala?Thr?Ile?Glu?Asn?Ile?Ser?Thr?Gly?Lys?Lys?Ala?Thr

180 185 190

Gln?Thr?Ile?Arg?Ala?Pro?Ala?Ala?Thr?Ala?Thr?Leu?Ala?Gly?Gln?Asn

195 200 205

Ala?Asp?Trp?Ile?Val?Glu?Asp?Phe?Gln?Ser?Gly?Asp?Ser?Met?Val?Asp

210 215 220

Leu?Ala?Gly?Phe?Gly?Glu?Ile?Ser?Phe?Trp?Gly?Val?Gln?Ala?Gln?Gly

225 230 235 240

Gly?Gly?Ser?Thr?Trp?Gly?Val?Asp?Asp?Ala?Thr?Ile?Val?Glu?Leu?Lys

245 250 255

Gln?Gly?Asn?Glu?Val?Leu?Thr?Asp?Val?Glu?Val?Gln?Ser?Asp?Ser?Ala

260 265 270

Phe?Thr?Val?Lys?Tyr?Thr?Ser

275

<210>123

<211>573

<212>PRT

＜213〉aspergillus niger

<400>123

Met?Ile?Tyr?Val?Asn?Tyr?Ile?Leu?Gly?Leu?Leu?Ser?Leu?Leu?His?Thr

1 5 10 15

Ala?Val?Ala?Thr?Ala?Pro?Asp?Tyr?Val?Val?Val?Asp?Gln?Leu?Asn?Ser

20 25 30

Ile?Pro?Asp?Gly?Trp?Thr?Lys?Gly?Ala?Ala?Pro?Pro?Pro?Phe?Thr?Pro

35 40 45

Met?Lys?Phe?Trp?Leu?Ser?Met?His?His?Glu?Tyr?Lys?Ala?Asp?Phe?Glu

50 55 60

Gln?Lys?Val?Ile?Asp?Ile?Ser?Thr?Pro?Gly?His?Arg?Asp?Tyr?Gly?Arg

65 70 75 80

His?Met?Lys?Arg?Asn?Asp?Val?Met?Ala?Phe?Met?Arg?Pro?Ser?Asp?Gln

85 90 95

Val?Ser?Lys?Ile?Ile?Phe?Ser?Trp?Leu?Glu?Ser?Glu?His?Val?Pro?Pro

100 105 110

Asn?Ala?Ile?Glu?Asp?Arg?Gly?Asp?Trp?Val?Ala?Phe?Thr?Val?Pro?Leu

115 120 125

Ala?Gln?Ala?Gln?Ser?Met?Met?Lys?Thr?Asp?Phe?Tyr?Asn?Phe?His?His

130 135 140

Leu?Glu?Thr?Asn?Thr?Thr?Gln?Ile?Arg?Thr?Leu?Lys?Tyr?Ser?Val?Pro

145 150 155 160

Glu?Gln?Val?Asp?Ala?His?Leu?Gln?Met?Ile?Gln?Pro?Thr?Thr?Arg?Phe

165 170 175

Gly?Arg?Pro?Lys?Thr?Gln?Thr?Ser?Leu?Pro?Ser?Leu?Met?Pro?Val?Ser

180 185 190

Val?Asn?Ile?Asp?Glu?Ile?Ser?Glu?Asp?Cys?Leu?Thr?Gly?Val?Thr?Pro

195 200 205

Ile?Cys?Leu?Arg?Gln?Leu?Tyr?Gly?Leu?Pro?Ser?Thr?Lys?Ala?Ser?Pro

210 215 220

Asp?Ser?Arg?Asn?Val?Leu?Gly?Ile?Ser?Gly?Tyr?Leu?Asp?Gln?Tyr?Ala

225 230 235 240

Arg?Tyr?Ser?Asp?Leu?Asp?Glu?Phe?Leu?Ala?Val?Tyr?Ser?Pro?Asn?Ser

245 250 255

Val?Asp?Ala?Asp?Phe?Ser?Val?Val?Ser?Ile?Asn?Gly?Gly?Gln?Asn?Pro

260 265 270

Gln?Asn?Ser?Gln?Glu?Gly?Ser?Thr?Glu?Ala?Ser?Leu?Asp?Ile?Gln?Tyr

275 280 285

Ala?Leu?Ser?Met?Ala?Phe?Asp?Ala?Asn?Ala?Thr?Phe?Tyr?Thr?Thr?Ala

290 295 300

Gly?Arg?Ala?Pro?Ser?Pro?Tyr?Leu?Glu?Gln?Leu?Gln?Tyr?Leu?Val?Gly

305 310 315 320

Leu?Pro?Asp?Glu?Asp?Leu?Pro?Ala?Val?Leu?Ser?Thr?Ser?Tyr?Gly?Glu

325 330 335

Asp?Glu?Gln?Ser?Leu?Pro?Glu?Glu?Tyr?Thr?Glu?Ala?Thr?Cys?Asn?Leu

340 345 350

Phe?Ala?Gln?Leu?Gly?Ala?Arg?Gly?Val?Ser?Val?Ile?Phe?Ser?Ser?Gly

355 360 365

Asp?Ser?Gly?Val?Gly?Gly?Ser?Cys?Val?Ser?Asn?Asp?Gly?Ser?Gln?Arg

370 375 380

Thr?Arg?Phe?Gln?Pro?Ile?Phe?Pro?Ala?Ser?Cys?Pro?Phe?Val?Thr?Ser

385 390 395 400

Val?Gly?Gly?Thr?Glu?Gly?Val?Gly?Pro?Glu?Lys?Ala?Val?Asp?Phe?Ser

405 410 415

Ser?Gly?Gly?Phe?Ser?Glu?Arg?Phe?Ala?Arg?Pro?Ser?Tyr?Gln?Asn?Ala

420 425 430

Ser?Val?Glu?Ala?Tyr?Leu?Ala?Arg?Leu?Gly?Asp?Lys?Trp?Asp?Gly?Leu

435 440 445

Tyr?Asn?Pro?Asp?Gly?Arg?Gly?Ile?Pro?Asp?Val?Ser?Ala?Gln?Ala?Ser

450 455 460

Asn?Tyr?Val?Ile?Arg?Asp?His?Gly?Gln?Trp?Leu?Gln?Thr?Ala?Gly?Thr

465 470 475 480

Ser?Ala?Ala?Ala?Pro?Val?Phe?Ala?Ala?Val?Ile?Ser?Arg?Leu?Asn?Ala

485 490 495

Ala?Arg?Leu?Glu?Gln?Gly?Lys?Pro?Thr?Leu?Gly?Phe?Leu?Asn?Pro?Trp

500 505 510

Leu?Tyr?Ser?Leu?Asp?Gln?Gln?Gly?Phe?Thr?Asp?Ile?Val?Asp?Gly?Gly

515 520 525

Ser?Val?Gly?Cys?Asp?Gly?Ser?Asn?Gly?Gly?Ala?Leu?Val?Pro?Tyr?Ala

530 535 540

Ser?Trp?Asn?Ala?Thr?Lys?Gly?Trp?Asp?Pro?Val?Thr?Gly?Leu?Gly?Thr

545 550 555 560

Pro?Leu?Tyr?Gln?Thr?Leu?Glu?Gln?Leu?Ala?Gln?Ser?Ala

565 570

<210>124

<211>585

<212>PRT

＜213〉aspergillus niger

<400>124

Met?Arg?Ser?Ser?Gly?Leu?Tyr?Thr?Ala?Leu?Leu?Cys?Ser?Leu?Ala?Ala

1 5 10 15

Ser?Thr?Asn?Ala?Ile?Val?His?Glu?Lys?Leu?Ala?Ala?Val?Pro?Ser?Gly

20 25 30

Trp?His?His?Val?Glu?Asp?Ala?Gly?Ser?Asp?His?Gln?Ile?Ser?Leu?Ser

35 40 45

Ile?Ala?Leu?Ala?Arg?Lys?Asn?Leu?Asp?Gln?Leu?Glu?Ser?Lys?Leu?Lys

50 55 60

Asp?Leu?Ser?Thr?Pro?Gly?Glu?Ser?Gln?Tyr?Gly?Gln?Trp?Leu?Asp?Gln

65 70 75 80

Glu?Asp?Val?Asp?Thr?Leu?Phe?Pro?Val?Ala?Ser?Asp?Lys?Ala?Val?Ile

85 90 95

Asn?Trp?Leu?Arg?Ser?Ala?Asn?Ile?Thr?His?Ile?Ser?Arg?Gln?Gly?Ser

100 105 110

Leu?Val?Asn?Phe?Ala?Thr?Thr?Val?Asp?Lys?Val?Asn?Lys?Leu?Leu?Asn

115 120 125

Ala?Thr?Phe?Ala?Tyr?Tyr?Gln?Ser?Gly?Ser?Ser?Gln?Arg?Leu?Arg?Thr

130 135 140

Thr?Glu?Tyr?Ser?Ile?Pro?Asp?Asp?Leu?Val?Asp?Ser?Ile?Asp?Leu?Ile

145 150 155 160

Ser?Pro?Thr?Thr?Phe?Phe?Gly?Lys?Glu?Lys?Thr?Thr?Ala?Gly?Leu?Asn

165 170 175

Gln?Arg?Ala?Gln?Lys?Ile?Asp?Thr?His?Val?Ala?Lys?Arg?Ser?Asn?Ser

180 185 190

Ser?Ser?Cys?Ala?Asp?Val?Ile?Thr?Leu?Ser?Cys?Leu?Lys?Glu?Met?Tyr

195 200 205

Asn?Phe?Gly?Asn?Tyr?Thr?Pro?Ser?Ala?Ser?Ser?Gly?Ser?Lys?Leu?Gly

210 215 220

Phe?Gly?Ser?Phe?Leu?Asn?Glu?Ser?Ala?Ser?Tyr?Ser?Asp?Leu?Ala?Lys

225 230 235 240

Phe?Glu?Lys?Leu?Phe?Asn?Leu?Pro?Ser?Gln?Ser?Phe?Ser?Val?Glu?Leu

245 250 255

Val?Asn?Gly?Gly?Val?Asn?Asp?Gln?Asn?Gln?Ser?Thr?Ala?Ser?Leu?Thr

260 265 270

Glu?Ala?Asp?Leu?Asp?Val?Glu?Leu?Leu?Val?Gly?Val?Ala?His?Pro?Leu

275 280 285

Pro?Val?Thr?Glu?Phe?Ile?Thr?Ser?Gly?Glu?Pro?Ala?Ala?Asp?Asn?Glu

290 295 300

Asn?Glu?Pro?Tyr?Leu?Gln?Tyr?Tyr?Glu?Tyr?Leu?Leu?Ser?Lys?Pro?Asn

305 310 315 320

Ser?Ala?Leu?Pro?Gln?Val?Ile?Ser?Asn?Ser?Tyr?Gly?Asp?Asp?Glu?Gln

325 330 335

Thr?Val?Pro?Glu?Tyr?Tyr?Ala?Lys?Arg?Val?Cys?Asn?Leu?Ile?Gly?Leu

340 345 350

Val?Gly?Leu?Arg?Gly?Ile?Ser?Val?Leu?Glu?Ser?Ser?Gly?Asp?Glu?Gly

355 360 365

Ile?Gly?Ser?Gly?Cys?Arg?Thr?Thr?Asp?Gly?Thr?Asn?Arg?Thr?Gln?Phe

370 375 380

Asn?Pro?Ile?Phe?Pro?Ala?Thr?Cys?Pro?Tyr?Val?Thr?Ala?Val?Gly?Gly

385 390 395 400

Thr?Met?Ser?Tyr?Ala?Pro?Glu?Ile?Ala?Trp?Glu?Ala?Ser?Ser?Gly?Gly

405 410 415

Phe?Ser?Asn?Tyr?Phe?Glu?Arg?Ala?Trp?Phe?Gln?Lys?Glu?Ala?Val?Gln

420 425 430

Asn?Tyr?Leu?Ala?His?His?Ile?Thr?Asn?Glu?Thr?Lys?Gln?Tyr?Tyr?Ser

435 440 445

Gln?Phe?Ala?Asn?Phe?Ser?Gly?Arg?Gly?Phe?Pro?Asp?Val?Ala?Ala?His

450 455 460

Ser?Phe?Glu?Pro?Ser?Tyr?Glu?ValIle?Phe?Tyr?Gly?Ala?Arg?Tyr?Gly

465 470 475 480

Ser?Gly?Gly?Thr?Ser?Ala?Ala?Cys?Pro?Leu?Phe?Ser?Ala?Leu?Val?Gly

485 490 495

Met?Leu?Asn?Asp?Ala?Arg?Leu?Arg?Ala?Gly?Lys?Ser?Thr?Leu?Gly?Phe

500 505 510

Leu?Asn?Pro?Leu?Leu?Tyr?Ser?Lys?Gly?Tyr?Arg?Ala?Leu?Thr?Asp?Val

515 520 525

Thr?Gly?Gly?Gln?Ser?Ile?Gly?Cys?Asn?Gly?Ile?Asp?Pro?Gln?Asn?Asp

530 535 540

Glu?Thr?Val?Ala?Gly?Ala?Gly?Ile?Ile?Pro?Trp?Ala?His?Trp?Asn?Ala

545 550 555 560

Thr?Val?Gly?Trp?Asp?Pro?Val?Thr?Gly?Leu?Gly?Leu?Pro?Asp?Phe?Glu

565 570 575

Lys?Leu?Arg?Gln?Leu?Val?Leu?Ser?Leu

580 585

<210>125

<211>265

<212>PRT

＜213〉aspergillus niger

<400>125

Met?Lys?Thr?Thr?Ala?Leu?Leu?Thr?Ala?Gly?Leu?Leu?Ala?Thr?Thr?Ala

1 5 10 15

Met?Ala?Ala?Pro?Leu?Thr?Ala?Lys?Arg?Gln?Ala?Ala?Arg?Ala?Lys?Arg

20 25 30

Ser?Thr?Asn?Arg?Gln?Ser?Asn?Pro?Pro?Phe?Lys?Pro?Gly?Thr?Asn?Glu

35 40 45

Val?Leu?Ala?Leu?Asn?Gly?Thr?Lys?Asn?Val?Glu?Tyr?Ser?Ser?Asn?Trp

50 55 60

Ala?Gly?Ala?Val?Leu?Ile?Gly?Thr?Gly?Tyr?Thr?Ala?Val?Thr?Ala?Glu

65 70 75 80

Phe?Val?Val?Pro?Thr?Pro?Ser?Val?Pro?Ser?Gly?Gly?Ser?Ser?Arg?Glu

85 90 95

Glu?Tyr?Cys?Ala?Ser?Ala?Trp?Val?Gly?Ile?Asp?Gly?Asp?Thr?Cys?Asp

100 105 110

Thr?Ala?Ile?Leu?Gln?Thr?Gly?Val?Asp?Phe?Cys?Val?Gln?Gly?Ser?Glu

115 120 125

Val?Ser?Phe?Asp?Ala?Trp?Tyr?Glu?Trp?Tyr?Pro?Asp?Tyr?Ala?Tyr?Asp

130 135 140

Phe?Ser?Gly?Ile?Ser?Ile?Ser?Ala?Gly?Asp?Thr?Ile?Lys?Val?Thr?Val

145 150 155 160

Asp?Ala?Ser?Ser?Asp?Thr?Thr?Gly?Thr?Ala?Thr?Ile?Glu?Asn?Val?Ser

165 170 175

Thr?Gly?Thr?Thr?Val?Thr?His?Ser?Phe?Thr?Gly?Gly?Val?Asp?Gly?Asp

180 185 190

Leu?Cys?Glu?Tyr?Asn?Ala?Glu?Trp?Ile?Val?Glu?Asp?Phe?Glu?Glu?Asp

195 200 205

Asp?Ser?Leu?Val?Pro?Phe?Ala?Asp?Phe?Gly?Thr?Val?Thr?Phe?Thr?Ser

210 215 220

Cys?Ser?Ala?Thr?Lys?Asp?Gly?Ser?Ser?Val?Gly?Pro?Glu?Asp?Ala?Thr

225 230 235 240

Ile?Ile?Asp?Ile?Glu?Gln?Asn?Glu?Val?Leu?Thr?Ser?Val?Ser?Val?Ser

245 250 255

Ser?Ser?Glu?Val?Val?Val?Lys?Tyr?Val

260 265

<210>126

<211>580

<212>PRT

＜213〉aspergillus niger

<400>126

Met?Val?Ala?Phe?Ser?Arg?Ile?Ser?Ala?Gly?Phe?Ala?Leu?Ala?Ala?Pro

1 5 10 15

Ala?Leu?Ala?Ser?Val?Val?Leu?Glu?Thr?Val?Lys?Ser?Val?Pro?Ser?Asp

20 25 30

Trp?Lys?Leu?Val?Glu?Ala?Ala?Asp?Thr?Ser?Ser?Thr?Ile?Ser?Leu?Ser

35 40 45

Val?Ala?Leu?Ala?Arg?Gln?Asn?Leu?Asp?Gln?Leu?Glu?Glu?Lys?Leu?Leu

50 55 60

Ala?Val?Ser?Thr?Pro?Gly?Lys?Asp?Thr?Tyr?Gly?Gln?Phe?Leu?Asp?Leu

65 70 75 80

Asp?Asp?Ile?Asn?Glu?Gln?Phe?Pro?Leu?Ala?Asp?Asp?Ala?Ala?Val?Val

85 90 95

Ala?Trp?Leu?Lys?Lys?Ala?Gly?Val?Thr?Gln?Ile?His?Lys?Glu?Gly?Gly

100 105 110

Leu?Leu?Asn?Phe?Ala?Thr?Thr?Val?Gly?Thr?Ala?Asn?Gln?Leu?Leu?Asn

115 120 125

Thr?Thr?Phe?Ser?Val?Tyr?Lys?Ser?Gly?Ser?Thr?Gln?Lys?Leu?Arg?Thr

130 135 140

Thr?Gln?Tyr?Ser?Val?Pro?Asp?Glu?Leu?Thr?Gly?Ser?Ile?Asp?Leu?Ile

145 150 155 160

Ser?Pro?Thr?Val?Phe?Phe?Gly?Lys?Ser?Asn?Ala?Ala?Arg?Ser?Ala?Ala

165 170 175

Val?Arg?Ala?Ser?Gln?Thr?Thr?Lys?Glu?Thr?Ser?Arg?Lys?Lys?Ser?Ser

180 185 190

Asn?Val?Cys?Glu?Tyr?Ile?Thr?Pro?Asp?Cys?Leu?Lys?Glu?Gln?Tyr?Ser

195 200 205

Ile?Asp?Tyr?Thr?Pro?Glu?Ala?Ser?Ser?Gly?Ser?Arg?Val?Gly?Phe?Gly

210 215 220

Ser?Phe?Leu?Asn?Glu?Ser?Ala?Leu?Tyr?Ser?Asp?Leu?Asp?Leu?Phe?Thr

225 230 235 240

Gln?Tyr?Phe?Asp?Ile?Pro?Gln?Gln?Ser?Phe?Thr?Val?Glu?Thr?Ile?Asn

245 250 255

Gly?Gly?Ile?Asn?Asn?Gln?Glu?Asn?Asp?Pro?Asp?Gly?Glu?Ala?Asp?Leu

260 265 270

Asp?Val?Gln?Asn?Ile?Val?Gly?Ile?Ser?His?Pro?Leu?Pro?Val?Thr?Glu

275 280 285

Tyr?Ile?Thr?Gly?Gly?Ser?Pro?Pro?Phe?Ile?Pro?Asp?Val?Glu?Thr?Thr

290 295 300

Thr?Asp?Glu?Asn?Glu?Pro?Tyr?Leu?Gln?Tyr?Tyr?Glu?Tyr?Leu?Leu?Ala

305 310 315 320

Lys?Thr?Asn?Asp?Glu?Leu?Pro?Leu?Val?Ile?Ser?Asn?Ser?Tyr?Gly?Asp

325 330 335

Asp?Glu?Asp?Thr?Val?Pro?Ile?Ala?Tyr?Ala?Thr?Arg?Val?Cys?Asn?Leu

340 345 350

Ile?Gly?Leu?Met?Gly?Thr?Arg?Gly?Ile?Ser?Ile?Leu?Glu?Ser?Ser?Gly

355 360 365

Asp?Ser?Gly?Val?Gly?Gly?Ala?Cys?Met?Ser?Asn?Asp?Gly?Thr?Asp?Lys

370 375 380

Thr?Glu?Phe?Thr?Pro?Met?Phe?Pro?Gly?Thr?Cys?Pro?Tyr?Ile?Thr?Ala

385 390 395 400

Val?Gly?Gly?Thr?Gln?Asp?Val?Pro?Glu?Val?Ala?Trp?Val?Asp?Ser?Ser

405 410 415

Gly?Gly?Phe?Ser?Asn?Tyr?Phe?Ser?Gln?Pro?Ser?Tyr?Gln?Ser?Asp?Gln

420 425 430

Val?Glu?Thr?Tyr?Leu?Asp?Lys?Tyr?Ile?Ser?Ala?Ser?Thr?Lys?Lys?Tyr

435 440 445

Tyr?Glu?Gln?Tyr?Thr?Asn?Phe?Ser?Gly?Arg?Ala?Phe?Pro?Asp?Val?Ser

450 455 460

Ala?Phe?Ala?Gly?Ser?Pro?Tyr?Tyr?Glu?Thr?Tyr?Ile?Asp?Gly?Gln?Leu

465 470 475 480

Gly?Leu?Val?Ala?Gly?Thr?Ser?Gly?Ala?Ser?Pro?Val?Phe?Ala?Gly?Ile

485 490 495

Val?Ala?Leu?Leu?Asn?Asp?Ala?Arg?Leu?Arg?Ala?Asn?Lys?Thr?Ser?Leu

500 505 510

Gly?Phe?Leu?Asn?Pro?Trp?Leu?Tyr?Ser?Ser?Gly?Tyr?Lys?Ser?Leu?Asn

515 520 525

Asp?Ile?Thr?Ser?Gly?Glu?Ala?Val?Gly?Cys?Gln?Gly?Asp?Val?Glu?Gly

530 535 540

Ala?Gly?Val?Ile?Pro?Trp?Ala?Ser?Trp?Asn?Ala?Thr?Thr?Gly?Trp?Asp

545 550 555 560

Pro?Ala?Thr?Gly?Leu?Gly?Thr?Pro?Asn?Phe?Ala?Lys?Leu?Lys?Glu?Ala

565 570 575

Val?Leu?Ala?Leu

580

<210>127

<211>631

<212>PRT

＜213〉aspergillus niger

<400>127

Met?His?Gly?Leu?Arg?Leu?Val?Cys?Ser?Ile?Gly?Thr?Leu?Pro?Leu?Val

1 5 10 15

Ile?Leu?Ala?Tyr?Pro?Ala?Ala?Ser?Leu?His?Thr?Thr?Ser?Ala?Ala?Val

20 25 30

Asp?Leu?Asp?Ser?Leu?Arg?Leu?Thr?Ser?Asn?Ser?Glu?Tyr?Val?Asn?Ser

35 40 45

Val?His?Val?Asp?Thr?Asn?Arg?Ser?Val?Ala?Val?Ser?Ala?Glu?Glu?His

50 55 60

Tyr?Thr?Asp?Thr?Ala?Ala?Arg?Leu?Val?Gln?Asn?Ile?Val?Pro?Gly?Ala

65 70 75 80

Ser?Phe?Arg?Leu?Ile?Asp?Asp?His?Phe?Val?Gly?Asp?Asn?Gly?Val?Ala

85 90 95

His?Val?Tyr?Phe?Arg?Gln?Thr?Leu?His?Gly?Ile?Asp?Ile?Asp?Asn?Ala

100 105 110

Asp?Phe?Asn?Val?Asn?Ile?Gly?Lys?Asp?Gly?Leu?Val?Leu?Ser?Phe?Gly

115 120 125

His?Ser?Phe?Phe?Thr?Gly?Ala?Leu?Pro?Ser?Ser?His?Leu?Asp?Asn?Thr

130 135 140

Asn?Val?Leu?Ser?Pro?Glu?Ala?Ala?Leu?Arg?Gly?Ala?Arg?Asp?Ala?Ile

145 150 155 160

Gln?Leu?Pro?Leu?Thr?Ile?Asp?Asn?Val?Ser?Thr?Glu?Ala?Ala?Glu?Gly

165 170 175

Arg?Asn?Glu?Tyr?Ile?Phe?Arg?Glu?Ala?Val?Gly?Ala?Val?Ser?Asp?Pro

180 185 190

Lys?Ala?Lys?Leu?Val?Tyr?Leu?Val?Lys?Pro?Glu?Gly?Thr?Leu?Ala?Leu

195 200 205

Thr?Trp?Arg?Ile?Glu?Thr?Asp?Met?Tyr?Glu?His?Trp?Leu?Leu?Thr?Tyr

210 215 220

Ile?Asp?Ala?Glu?Thr?Thr?Thr?Val?His?Gly?Val?Val?Asp?Tyr?Val?Ala

225 230 235 240

Asp?Ala?Thr?Tyr?Gln?Val?Tyr?Pro?Trp?Gly?Thr?Asn?Asp?Pro?Ala?Glu

245 250 255

Gly?His?Arg?Thr?Ile?Val?Thr?Asp?Pro?Trp?Asp?Leu?Ser?Ala?Ser?Ala

260 265 270

Tyr?Thr?Trp?Ile?Ser?Asp?Gly?Arg?Asp?Asn?Tyr?Thr?Thr?Thr?Arg?Gly

275 280 285

Asn?Asn?Ala?Ile?Ala?His?Trp?Asn?Pro?Thr?Gly?Gly?Gly?Ser?Tyr?Leu

290 295 300

Tyr?Asn?Leu?Arg?Pro?Ser?Asp?Pro?Asn?Leu?Asn?Phe?Gln?Trp?Pro?Tyr

305 310 315 320

Ser?Pro?Asn?Met?Ser?Pro?Pro?Arg?Ser?Tyr?Ile?Asn?Ala?Ser?Ile?Val

325 330 335

Gln?Leu?Phe?Tyr?Thr?Ala?Asn?Ala?Tyr?His?Asp?Leu?Leu?Tyr?Thr?Leu

340 345 350

Gly?Phe?Thr?Glu?Ser?Ala?Gly?Asn?Phe?Gln?Trp?Asn?Asn?Ser?Ala?His

355 360 365

Gly?Gly?Arg?Asp?Lys?Asp?Tyr?Val?Ile?Leu?Asn?Ala?Gln?Asp?Gly?Ser

370 375 380

Gly?Phe?Ser?Asn?Ala?Asn?Phe?Ala?Thr?Pro?Pro?Asp?Gly?Ile?Pro?Gly

385 390 395 400

Arg?Met?Arg?Met?Tyr?Ile?Trp?Ile?Glu?Ser?Thr?Pro?Ser?Arg?Asp?Gly

405 410 415

Ser?Phe?Asp?Ala?Gly?Ile?Val?Ile?His?Glu?Tyr?Thr?His?Gly?Val?Ser

420 425 430

Asn?Arg?Leu?Thr?Gly?Gly?Ser?His?Asn?Ala?Gly?Cys?Leu?Ser?Ala?Leu

435 440 445

Glu?Ser?Gly?Gly?Met?Gly?Glu?Gly?Trp?Gly?Asp?Phe?Met?Ala?Thr?Ala

450 455 460

Ile?Arg?Ile?Lys?Pro?Asn?Asp?Thr?Arg?Thr?Thr?Ser?Tyr?Thr?Met?Gly

465 470 475 480

Ala?Trp?Ala?Asp?Asn?Asp?Lys?Cys?Gly?Val?Arg?Asp?Tyr?Pro?Tyr?Ser

485 490 495

Thr?Ser?Phe?Thr?Glu?Asn?Pro?Leu?Asn?Tyr?Thr?Ser?Val?Asn?Thr?Met

500 505 510

Asn?Gly?Val?His?Ala?Ile?Gly?Thr?Val?Trp?Ala?Thr?Met?Leu?Tyr?Glu

515 520 525

Val?Leu?Trp?Asn?Leu?Ile?Asp?Lys?Tyr?Gly?Lys?Asn?Asp?Gly?Ser?Arg

530 535 540

Pro?Val?Phe?Arg?Asn?Gly?Val?Pro?Thr?Asp?Gly?Lys?Tyr?Leu?Met?Met

545 550 555 560

Lys?Leu?Val?Val?Asp?Gly?Met?Ala?Leu?Gln?Pro?Cys?Asn?Pro?Asn?Phe

565 570 575

Val?Gln?Ala?Arg?Asp?Ala?Ile?Leu?Asp?Ala?Asp?Ile?Val?Leu?Thr?Gly

580 585 590

Gly?Lys?Asn?Arg?Cys?Glu?Ile?Trp?Arg?Gly?Phe?Ala?Lys?Arg?Gly?Leu

595 600 605

Gly?Gln?Gly?Ala?Ala?His?Ser?Ser?Leu?Asn?Trp?Met?Arg?Arg?Gly?Ser

610 615 620

Thr?Leu?Leu?Pro?Thr?Gly?Cys

625 630

<210>128

<211>394

<212>PRT

＜213〉aspergillus niger

<400>128

Met?Val?Val?Phe?Ser?Lys?Thr?Ala?Ala?Leu?Val?Leu?Gly?Leu?Ser?Ser

1 5 10 15

Ala?Val?Ser?Ala?Ala?Pro?Ala?Pro?Thr?Arg?Lys?Gly?Phe?Thr?Ile?Asn

20 25 30

Gln?Ile?Ala?Arg?Pro?Ala?Asn?Lys?Thr?Arg?Thr?Ile?Asn?Leu?Pro?Gly

35 40 45

Met?Tyr?Ala?Arg?Ser?Leu?Ala?Lys?Phe?Gly?Gly?Thr?Val?Pro?Gln?Ser

50 55 60

Val?Lys?Glu?Ala?Ala?Ser?Lys?Gly?Ser?Ala?Val?Thr?Thr?Pro?Gln?Asn

65 70 75 80

Asn?Asp?Glu?Glu?Tyr?Leu?Thr?Pro?Val?Thr?Val?Gly?Lys?Ser?Thr?Leu

85 90 95

His?Leu?Asp?Phe?Asp?Thr?Gly?Ser?Ala?Asp?Leu?Trp?Val?Phe?Ser?Asp

100 105 110

Glu?Leu?Pro?Ser?Ser?Glu?Gln?Thr?Gly?His?Asp?Leu?Tyr?Thr?Pro?Ser

115 120 125

Ser?Ser?Ala?Thr?Lys?Leu?Ser?Gly?Tyr?Thr?Trp?Asp?Ile?Ser?Tyr?Gly

130 135 140

Asp?Gly?Ser?Ser?Ala?Ser?Gly?Asp?Val?Tyr?Arg?Asp?Thr?Val?Thr?Val

145 150 155 160

Gly?Gly?Val?Thr?Thr?Asn?Lys?Gln?Ala?Val?Glu?Ala?Ala?Ser?Lys?Ile

165 170 175

Ser?Ser?Glu?Phe?Val?Gln?Asn?Thr?Ala?Asn?Asp?Gly?Leu?Leu?Gly?Leu

180 185 190

Ala?Phe?Ser?Ser?Ile?Asn?Thr?Val?Gln?Pro?Lys?Ala?Gln?Thr?Thr?Phe

195 200 205

Phe?Asp?Thr?Val?Lys?Ser?Gln?Leu?Asp?Ser?Pro?Leu?Phe?Ala?Val?Gln

210 215 220

Leu?Lys?His?Asp?Ala?Pro?Gly?Val?Tyr?Asp?Phe?Gly?Tyr?Ile?Asp?Asp

225 230 235 240

Ser?Lys?Tyr?Thr?Gly?Ser?Ile?Thr?Tyr?Thr?Asp?Ala?Asp?Ser?Ser?Gln

245 250 255

Gly?Tyr?Trp?Gly?Phe?Ser?Thr?Asp?Gly?Tyr?Ser?Ile?Gly?Asp?Gly?Ser

260 265 270

Ser?Ser?Ser?Ser?Gly?Phe?Ser?Ala?Ile?Ala?Asp?Thr?Gly?Thr?Thr?Leu

275 280 285

Ile?Leu?Leu?Asp?Asp?Glu?Ile?Val?Ser?Ala?Tyr?Tyr?Glu?Gln?Val?Ser

290 295 300

Gly?Ala?Gln?Glu?Ser?Glu?Glu?Ala?Gly?Gly?Tyr?Val?Phe?Ser?Cys?Ser

305 310 315 320

Thr?Asn?Pro?Pro?Asp?Phe?Thr?Val?Val?Ile?Gly?Asp?Tyr?Lys?Ala?Val

325 330 335

Val?Pro?Gly?Lys?Tyr?Ile?Asn?Tyr?Ala?Pro?Ile?Ser?Thr?Gly?Ser?Ser

340 345 350

Thr?Cys?Phe?Gly?Gly?Ile?Gln?Ser?Asn?Ser?Gly?Leu?Gly?Leu?Ser?Ile

355 360 365

Leu?Gly?Asp?Val?Phe?Leu?Lys?Ser?Gln?Tyr?Val?Val?Phe?Asn?Ser?Glu

370 375 380

Gly?Pro?Lys?Leu?Gly?Phe?Ala?Ala?Gln?Ala

385 390

<210>129

<211>398

<212>PRT

＜213〉aspergillus niger

<400>129

Met?Lys?Ser?Ala?Ser?Leu?Leu?Thr?Ala?Ser?Val?Leu?Leu?Gly?Cys?Ala

1 5 10 15

Ser?Ala?Glu?Val?His?Lys?Leu?Lys?Leu?Asn?Lys?Val?Pro?Leu?Glu?Glu

20 25 30

Gln?Leu?Tyr?Thr?His?Asn?Ile?Asp?Ala?His?Val?Arg?Ala?Leu?Gly?Gln

35 40 45

Lys?Tyr?Met?Gly?Ile?Arg?Pro?Ser?Ile?His?Lys?Glu?Leu?Val?Glu?Glu

50 55 60

Asn?Pro?Ile?Asn?Asp?Met?Ser?Arg?His?Asp?Val?Leu?Val?Asp?Asn?Phe

65 70 75 80

Leu?Asn?Ala?Gln?Tyr?Phe?Ser?Glu?Ile?Glu?Leu?Gly?Thr?Pro?Pro?Gln

85 90 95

Lys?Phe?Lys?Val?Val?Leu?Asp?Thr?Gly?Ser?Ser?Asn?Leu?Trp?Val?Pro

100 105 110

Ser?Ser?Glu?Cys?Ser?Ser?Ile?Ala?Cys?Tyr?Leu?His?Asn?Lys?Tyr?Asp

115 120 125

Ser?Ser?Ala?Ser?Ser?Thr?Tyr?His?Lys?Asn?Gly?Ser?Glu?Phe?Ala?Ile

130 135 140

Lys?Tyr?Gly?Ser?Gly?Ser?Leu?Ser?Gly?Phe?Ile?Ser?Gln?Asp?Thr?Leu

145 150 155 160

Lys?Ile?Gly?Asp?Leu?Lys?Val?Lys?Gly?Gln?Asp?Phe?Ala?Glu?Ala?Thr

165 170 175

Asn?Glu?Pro?Gly?Leu?Ala?Phe?Ala?Phe?Gly?Arg?Phe?Asp?Gly?Ile?Leu

180 185 190

Gly?Leu?Gly?Tyr?Asp?Thr?Ile?Ser?Val?Asn?Lys?Ile?Val?Pro?Pro?Phe

195 200 205

Tyr?Asn?Met?Leu?Asp?Gln?Gly?Leu?Leu?Asp?Glu?Pro?Val?Phe?Ala?Phe

210 215 220

Tyr?Leu?Gly?Asp?Thr?Asn?Lys?Glu?Gly?Asp?Glu?Ser?Val?Ala?Thr?Phe

225 230 235 240

Gly?Gly?Val?Asp?Lys?Asp?His?Tyr?Thr?Gly?Glu?Leu?Ile?Lys?Ile?Pro

245 250 255

Leu?Arg?Arg?Lys?Ala?Tyr?Trp?Glu?Val?Glu?Leu?Asp?Ala?Ile?Ala?Leu

260 265 270

Gly?Asp?Asp?Val?Ala?Glu?Met?Glu?Asn?Thr?Gly?Val?Ile?Leu?Asp?Thr

275 280 285

Gly?Thr?Ser?Leu?Ile?Ala?Leu?Pro?Ala?Asp?Leu?Ala?Glu?Met?Ile?Asn

290 295 300

Ala?Gln?Ile?Gly?Ala?Lys?Lys?Gly?Trp?Thr?Gly?Gln?Tyr?Thr?Val?Asp

305 310 315 320

Cys?Asp?Lys?Arg?Ser?Ser?Leu?Pro?Asp?Val?Thr?Phe?Thr?Leu?Ala?Gly

325 330 335

His?Asn?Phe?Thr?Ile?Ser?Ser?Tyr?Asp?Tyr?Thr?Leu?Glu?Val?Gln?Gly

340 345 350

Ser?Cys?Val?Ser?Ala?Phe?Met?Gly?Met?Asp?Phe?Pro?Glu?Pro?Val?Gly

355 360 365

Pro?Leu?Ala?Ile?Leu?Gly?Asp?Ala?Phe?Leu?Arg?Lys?Trp?Tyr?Ser?Val

370 375 380

Tyr?Asp?Leu?Gly?Asn?Ser?Ala?Val?Gly?Leu?Ala?Lys?Ala?Lys

385 390 395

<210>130

<211>393

<212>PRT

＜213〉aspergillus niger

<400>130

Met?Arg?Lys?Tyr?Arg?Phe?His?Pro?Thr?Lys?Pro?Gly?Pro?Tyr?Thr?Leu

1 5 10 15

Ser?Ser?Ser?Ile?Gln?Gln?Thr?Gly?Arg?Pro?Tyr?Thr?Glu?Lys?Pro?Ile

20 25 30

Gly?Gly?Arg?Ala?His?Ile?Arg?Gln?Leu?Val?Arg?Lys?Lys?Ser?Thr?Thr

35 40 45

Ser?Asp?Glu?Val?Gly?Glu?Val?Pro?Ala?Glu?Asp?Val?Gln?Asn?Asp?Ser

50 55 60

Met?Tyr?Leu?Ala?Thr?Val?GlyIle?Gly?Thr?Pro?Ala?Gln?Asn?Leu?Lys

65 70 75 80

Leu?Asp?Phe?Asp?Thr?Gly?Ser?Ala?Asp?Leu?Trp?Val?Trp?Ser?Asn?Lys

85 90 95

Leu?Pro?Ser?Thr?Leu?Leu?Ser?Glu?Asn?Lys?Thr?His?Ala?Ile?Phe?Asp

100 105 110

Ser?Ser?Lys?Ser?Ser?Thr?Phe?Lys?Thr?Leu?Glu?Gly?Glu?Ser?Trp?Gln

115 120 125

Ile?Ser?Tyr?Gly?Asp?Gly?Ser?Ser?Ala?Ser?Gly?Ser?Val?Gly?Thr?Asp

130 135 140

Asp?Val?Asn?Ile?Gly?Gly?Val?Val?Val?Lys?Asn?Gln?Ala?Val?Glu?Leu

145 150 155 160

Ala?Glu?Lys?Met?Ser?Ser?Thr?Phe?Ala?Gln?Gly?Glu?Gly?Asp?Gly?Leu

165 170 175

Leu?Gly?Leu?Ala?Phe?Ser?Asn?Ile?Asn?Thr?Val?Gln?Pro?Lys?Ser?Val

180 185 190

Lys?Thr?Pro?Val?Glu?Asn?Met?Ile?Leu?Gln?Asp?Asp?Ile?Pro?Lys?Ser

195 200 205

Ala?Glu?Leu?Phe?Thr?Ala?Lys?Leu?Asp?Thr?Trp?Arg?Asp?Thr?Asp?Asp

210 215 220

Glu?Ser?Phe?Tyr?Thr?Phe?Gly?Phe?Ile?Asp?Gln?Asp?Leu?Val?Lys?Thr

225 230 235 240

Ala?Gly?Glu?Glu?Val?Tyr?Tyr?Thr?Pro?Val?Asp?Asn?Ser?Gln?Gly?Phe

245 250 255

Trp?Leu?Phe?Asn?Ser?Thr?Ser?Ala?Thr?Val?Asn?Gly?Lys?Thr?Ile?Asn

260 265 270

Arg?Ser?Gly?Asn?Thr?Ala?Ile?Ala?Asp?Thr?Gly?Thr?Thr?Leu?Ala?Leu

275 280 285

Val?Asp?Asp?Asp?Thr?Cys?Glu?Ala?Ile?Tyr?Ser?Ala?Ile?Asp?Gly?Ala

290 295 300

Tyr?Tyr?Asp?Gln?Glu?Val?Gln?Gly?Trp?Ile?Tyr?Pro?Thr?Asp?Thr?Ala

305 310 315 320

Gln?Asp?Lys?Leu?Pro?Thr?Val?Ser?Phe?Ala?Val?Gly?Glu?Lys?Gln?Phe

325 330 335

Val?Val?Gln?Lys?Glu?Asp?Leu?Ala?Phe?Ser?Glu?Ala?Lys?Thr?Gly?Tyr

340 345 350

Val?Tyr?Gly?Gly?Ile?Gln?Ser?Arg?Gly?Asp?Met?Thr?Met?Asp?Ile?Leu

355 360 365

Gly?Asp?Thr?Phe?Leu?Lys?Ser?Ile?Tyr?Ala?Val?Ser?Ala?Leu?Leu?Leu

370 375 380

Ala?Leu?Arg?Gly?Asp?Ile?Glu?Ala?His

385 390

<210>131

<211>282

<212>PRT

＜213〉aspergillus niger

<400>131

Met?Lys?Phe?Ser?Thr?Ile?Leu?Thr?Gly?Ser?Leu?Phe?Ala?Thr?Ala?Ala

1 5 10 15

Leu?Ala?Ala?Pro?Leu?Thr?Glu?Lys?Arg?Arg?Ala?Arg?Lys?Glu?Ala?Arg

20 25 30

Ala?Ala?Gly?Lys?Arg?His?Ser?Asn?Pro?Pro?Tyr?Ile?Pro?Gly?Ser?Asp

35 40 45

Lys?Glu?Ile?Leu?Lys?Leu?Asn?Gly?Thr?Ser?Asn?Glu?Asp?Tyr?Ser?Ser

50 55 60

Asn?Trp?Ala?Gly?Ala?Val?Leu?Ile?Gly?Asp?Gly?Tyr?Thr?Lys?Val?Thr

65 70 75 80

Gly?Glu?Phe?Thr?Val?Pro?Ser?Val?Ser?Ala?Gly?Ser?Ser?Ser?Ser?Ser

85 90 95

Gly?Tyr?Gly?Gly?Gly?Tyr?Gly?Tyr?Tyr?Lys?Asn?Lys?Arg?Gln?Ser?Glu

100 105 110

Glu?Tyr?Cys?Ala?Ser?Ala?Trp?Val?Gly?Ile?Asp?Gly?Asp?Thr?Cys?Glu

115 120 125

Thr?Ala?Ile?Leu?Gln?Thr?Gly?Val?Asp?Phe?Cys?Tyr?Glu?Asp?Gly?Gln

130 135 140

Thr?Ser?Tyr?Asp?Ala?Trp?Tyr?Glu?Trp?Tyr?Pro?Asp?Tyr?Ala?Tyr?Asp

145 150 155 160

Phe?Asn?Asp?Ile?Thr?Ile?Ser?Glu?Gly?Asp?Thr?Ile?Lys?Val?Thr?Val

165 170 175

Glu?Ala?Thr?Ser?Lys?Ser?Ser?Gly?Ser?Ala?Thr?Val?Glu?Asn?Leu?Thr

180 185 190

Thr?Gly?Gln?Ser?Val?Thr?His?Thr?Phe?Ser?Gly?Asn?Val?Glu?Gly?Asp

195 200 205

Leu?Cys?Glu?Thr?Asn?Ala?Glu?Trp?Ile?Val?Glu?Asp?Phe?Glu?Ser?Gly

210 215 220

Asp?Ser?Leu?Val?Ala?Phe?Ala?Asp?Phe?Gly?Ser?Val?Thr?Phe?Thr?Asn

225 230 235 240

Ala?Glu?Ala?Thr?Ser?Asp?Gly?Ser?Thr?Val?Gly?Pro?Ser?Asp?Ala?Thr

245 250 255

Val?Met?Asp?Ile?Glu?Gln?Asp?Gly?Thr?Val?Leu?Thr?Glu?Thr?Ser?Val

260 265 270

Ser?Gly?Asp?Ser?Val?Thr?Val?Thr?Tyr?Val

275 280

<210>132

<211>273

<212>PRT

＜213〉aspergillus niger

<400>132

Met?Gly?Asp?Tyr?Gly?Pro?Gly?Val?Ser?Ser?Leu?Thr?Ala?Gln?Leu?Pro

1 5 10 15

Gly?Asn?Pro?Pro?Val?Ser?Glu?Thr?Asp?Gln?Asp?Glu?Ile?Ser?Val?Leu

20 25 30

Val?Thr?Gly?Phe?Gly?Pro?Phe?Lys?Ser?Asn?Leu?Val?Asn?Ala?Ser?Tyr

35 40 45

Leu?Ile?Ala?Ser?Ser?Leu?Pro?Pro?Ser?Phe?Thr?Phe?Ser?Pro?Ala?Ser

50 55 60

Ser?Asp?Gly?Ser?Asp?Ala?Val?Pro?Arg?Arg?Val?Ser?Ile?Asn?Val?His

65 70 75 80

Pro?Ser?Pro?Ile?Pro?Val?Ala?Tyr?Ser?Ser?Val?Arg?Thr?Thr?Leu?Pro

85 90 95

Val?Ile?Leu?Asp?Asp?Tyr?Ala?Lys?Thr?His?Gly?Gly?Arg?Arg?Pro?Asp

100 105 110

Ile?Val?Ile?His?Ile?Gly?Ile?Ala?Ala?Met?Arg?Asn?Tyr?Tyr?Ser?Val

115 120 125

Glu?Thr?Gln?Ala?His?Arg?Asp?Gly?Tyr?Leu?Met?Ser?Asp?Ile?Lys?Gly

130 135 140

Arg?Ser?Gly?Tyr?Glu?Asp?Gly?Glu?Lys?Leu?Trp?Arg?Glu?Leu?Asp?Leu

145 150 155 160

Pro?Leu?Val?Leu?Arg?Ala?Gly?Pro?Ser?Glu?Gly?His?Ala?Ser?Glu?Lys

165 170 175

Lys?His?Leu?Ser?Pro?Arg?Pro?Pro?Asp?Glu?Asp?Phe?Leu?Ala?Ala?Trp

180 185 190

Lys?Thr?Phe?Cys?Pro?Pro?Glu?Thr?Asp?Ala?Arg?Ile?Ser?Thr?Asp?Ala

195 200 205

Gly?Arg?Tyr?Leu?Cys?Glu?Phe?Ile?Leu?Tyr?Thr?Ser?Leu?Ala?Leu?Ala

210 215 220

Tyr?Gln?Ala?Gly?Glu?Asp?Arg?Asn?Val?Thr?Phe?Phe?His?Val?Pro?Ala

225 230 235 240

Ser?Cys?Leu?Asp?Glu?Asp?Ile?Glu?Thr?Gly?Lys?Glu?Val?Ala?Val?Ala

245 250 255

Leu?Ile?Lys?Ala?Leu?Val?Thr?Ser?Trp?Ser?Glu?Gln?Gln?His?Ser?Val

260 265 270

Pro

<210>133

<211>542

<212>PRT

＜213〉aspergillus niger

<400>133

Met?Gly?Ser?Arg?Gln?Gly?Lys?Ala?Pro?Phe?Gly?Trp?Gly?Thr?Gln?Ser

1 5 10 15

Leu?Ala?His?Phe?Gly?Ile?Asn?Pro?Asp?Leu?Gly?Leu?His?Asn?Gln?Gln

20 25 30

Asn?Leu?Asn?Ser?Leu?Ile?Ser?His?Ser?Ala?Met?Ala?Thr?Ala?Leu?Glu

35 40 45

Thr?Glu?Tyr?Ala?Thr?Ile?Pro?Ile?Asp?His?Asn?Asn?Ala?Ser?Ala?Gly

50 55 60

Thr?Tyr?Gln?Asn?Arg?Phe?Trp?Val?Ser?Asp?Glu?Phe?Tyr?Gln?Pro?Gly

65 70 75 80

Asn?Pro?Ile?Phe?Val?Tyr?Asp?Thr?Gly?Glu?Ser?Asp?Gly?Gly?Ser?Ile

85 90 95

Ala?Gln?Ser?Tyr?Leu?Thr?Ser?Thr?Leu?Ser?Phe?Phe?Arg?Glu?Phe?Leu

100 105 110

Ile?Glu?Phe?Asn?Ala?Met?Gly?Ile?Ala?Trp?Glu?His?Arg?Tyr?Tyr?Gly

115 120 125

Asn?Ser?Thr?Pro?Ala?Pro?Val?Ser?Tyr?Glu?Thr?Pro?Pro?Glu?Ala?Trp

130 135 140

Gln?Tyr?Leu?Thr?Thr?Lys?Gln?Ala?Leu?Ala?Asp?Leu?Pro?Tyr?Phe?Ala

145 150 155 160

Ser?Asn?Phe?Ser?Arg?Glu?Lys?Tyr?Pro?Asp?Met?Asp?Leu?Thr?Pro?Gln

165 170 175

Gly?Thr?Pro?Trp?Ile?Met?Val?Gly?Gly?Ser?Tyr?Ala?Gly?Ile?Arg?Ala

180 185 190

Ala?Leu?Thr?Arg?Lys?Glu?Tyr?Pro?Glu?Thr?Ile?Phe?Ala?Ala?Phe?Ser

195 200 205

Ser?Ser?Ser?Pro?Val?Glu?Ala?Gln?Val?Asn?Met?Ser?Ala?Tyr?Tyr?Asp

210 215 220

Gln?Val?Tyr?Arg?Gly?Met?Val?Ala?Ser?Gly?Trp?Thr?Asn?Cys?Ser?Ala

225 230 235 240

Asp?Ile?His?Ala?Ala?Leu?Glu?Tyr?Ile?Asp?Asp?Gln?Leu?Ser?Asp?Glu

245 250 255

Asp?Thr?Ala?Thr?Ser?Val?Lys?Gln?Leu?Phe?Phe?Gly?Ser?Gly?Ala?Glu

260 265 270

Thr?Asn?Ser?Asn?Gly?Asp?Phe?Thr?Ala?Ala?Leu?Thr?Ala?Ile?Tyr?Gly

275 280 285

Tyr?Phe?Gln?Ser?Tyr?Gly?Met?Ala?Gly?Gly?Ile?Gly?Gly?Leu?Gly?Ala

290 295 300

Phe?Cys?Glu?Tyr?Leu?Glu?Ile?Asp?Pro?Lys?Thr?Asn?Gly?Thr?Thr?Gly

305 310 315 320

Pro?Asp?Gly?Leu?Ala?Pro?Thr?Tyr?Gly?Gly?Gln?Tyr?Val?Ala?Glu?Arg

325 330 335

Trp?Ala?Ala?Trp?Pro?Thr?Phe?Leu?Glu?Leu?Val?Asn?Leu?Asn?Met?Gly

340 345 350

Thr?Asn?Cys?Gly?Pro?Gln?Asp?Ala?Ser?Gln?Pro?Ile?Asp?Cys?Asp?Phe

355 360 365

Ser?Lys?Pro?Tyr?Gly?Asp?Pro?Ser?Ala?Ile?Thr?Trp?Thr?Trp?Gln?Tyr

370 375 380

Cys?Ser?Glu?Trp?Gly?Phe?Phe?Gln?Ala?Asn?Asn?Asp?Gly?Pro?His?Ser

385 390 395 400

Leu?Ala?Ser?Arg?Tyr?Gln?Ser?Val?Glu?Tyr?Gln?Gln?Glu?Val?Cys?Asn

405 410 415

Arg?Gln?Phe?Pro?Asp?Ala?Val?Asp?Lys?Gly?Leu?Leu?Pro?Pro?Ser?Pro

420 425 430

Arg?Ala?Asp?Asp?Val?Asn?Gln?Glu?Phe?Gly?Gly?Trp?Thr?Ile?Arg?Pro

435 440 445

Ser?Asn?Val?Tyr?Phe?Ser?Gly?Gly?Glu?Phe?Asp?Pro?Trp?Arg?Ser?Leu

450 455 460

Ser?Ile?Leu?Ser?Thr?Glu?Asp?Phe?Ala?Pro?Gln?Gly?Val?Glu?Phe?Thr

465 470 475 480

Ser?Ala?Ile?Pro?Ala?Cys?Gly?Val?Gln?Thr?Asn?Glu?Asp?Thr?Val?Phe

485 490 495

Gly?Tyr?Val?Met?Gln?Asn?Ser?Glu?His?Cys?Phe?Asp?Phe?Gln?Ala?Thr

500 505 510

Pro?Thr?Val?Gly?Lys?Leu?Ser?Arg?Gly?Ile?Phe?Thr?Ser?Ala?Leu?Leu

515 520 525

Gln?Trp?Leu?Glu?Cys?Phe?Gly?Gln?Asn?Ser?Ser?Gln?Ser?Arg

530 535 540

<210>134

<211>391

<212>PRT

＜213〉aspergillus niger

<400>134

Met?Lys?Leu?Ser?Ile?Ala?Leu?Ala?Leu?Gly?Ala?Thr?Ala?Ser?Thr?Gly

1 5 10 15

Val?Leu?Ala?Ala?Val?Val?Pro?Gln?Gln?Glu?Pro?Leu?Ile?Thr?Pro?Gln

20 25 30

Asp?Pro?Pro?Thr?His?His?His?Gln?Glu?Lys?Phe?Leu?Ile?Glu?Leu?Ala

35 40 45

Pro?Tyr?Gln?Thr?Arg?Trp?Val?Thr?Glu?Glu?Glu?Lys?Trp?Asp?Leu?Lys

50 55 60

Leu?Asp?Gly?Val?Asn?Phe?Ile?Asp?Ile?Thr?Glu?Glu?Arg?Asn?Thr?Gly

65 70 75 80

Phe?Tyr?Pro?Thr?Leu?His?Ala?Gly?Ser?Tyr?Val?His?Tyr?Pro?Pro?Thr

85 90 95

Met?Lys?His?Ala?Glu?Lys?Val?Val?Pro?Leu?Leu?Arg?Gly?Leu?Ser?Lys

100 105 110

Asp?Asn?Met?Glu?Gln?Asn?Leu?Asn?Lys?Phe?Thr?Ser?Phe?His?Thr?Arg

115 120 125

Tyr?Tyr?Arg?Ser?Ser?Thr?Gly?Ile?Glu?Ser?Ala?Lys?Trp?Leu?Tyr?Ser

130 135 140

Arg?Val?Ser?Asp?Val?Ile?Glu?Gln?Ser?Gly?Ala?Ala?Glu?Tyr?Gly?Ala

145 150 155 160

Thr?Val?Glu?Gln?Phe?Ala?His?Ser?Trp?Gly?Gln?Phe?Ser?Ile?Ile?Ala

165 170 175

Arg?Ile?Pro?Gly?Gln?Thr?Asn?Lys?Thr?Val?Val?Leu?Gly?Ala?His?Gln

180 185 190

Asp?Ser?Ile?Asn?Leu?Phe?Leu?Pro?Ser?Ile?Leu?Ala?Ala?Pro?Gly?Ala

195 200 205

Asp?Asp?Asp?Gly?Ser?Gly?Thr?Val?Thr?Ile?Leu?Glu?Ala?Leu?Arg?Gly

210 215 220

Leu?Leu?Gln?Ser?Asp?Ala?Ile?Val?Arg?Gly?Asn?Ala?Ser?Asn?Thr?Ile

225 230 235 240

Glu?Phe?His?Trp?Tyr?Ser?Ala?Glu?Glu?Gly?Gly?Met?Leu?Gly?Ser?Gln

245 250 255

Ala?Ile?Phe?Ser?Gln?Tyr?Lys?Arg?Asp?Lys?Arg?Asp?Ile?Lys?Ala?Met

260 265 270

Leu?Gln?Gln?Asp?Met?Thr?Gly?Tyr?Thr?Gln?Gly?Ala?Leu?Asp?Ala?Gly

275 280 285

Arg?Gln?Glu?Ala?Ile?Gly?Ile?Met?Val?Asp?Tyr?Val?Asp?Glu?Gly?Leu

290 295 300

Thr?Gln?Phe?Leu?Lys?Asp?Val?Thr?Thr?Glu?Tyr?Cys?Gly?Ile?Gly?Tyr

305 310 315 320

Ile?Glu?Thr?Arg?Cys?Gly?Tyr?Ala?Cys?Ser?Asp?His?Thr?Ser?Ala?Ser

325 330 335

Lys?Tyr?Gly?Tyr?Pro?Ala?Ala?Met?Ala?Thr?Glu?Ser?Glu?Met?Glu?Asn

340 345 350

Ser?Asn?Lys?Arg?Ile?His?Thr?Thr?Asp?Asp?Ser?Ile?Arg?Tyr?Leu?Ser

355 360 365

Phe?Asp?His?Met?Leu?Glu?His?Ala?Arg?Leu?Thr?Leu?Gly?Phe?Ala?Tyr

370 375 380

Glu?Leu?Ala?Phe?Ala?Gln?Phe

385 390

<210>135

<211>442

<212>PRT

＜213〉aspergillus niger

<400>135

Met?Arg?Thr?Thr?Thr?Ser?Phe?Ala?Arg?Leu?Ala?Leu?Ala?Val?Ala?Ser

1 5 10 15

Val?Gly?Ile?Val?Phe?Ala?Ser?Pro?Thr?Lys?Asn?Asn?Asp?Gly?Lys?Leu

20 25 30

Val?Tyr?Gly?Ser?Pro?Glu?Ser?Val?Gly?Met?Ile?Ser?Ala?Pro?Leu?His

35 40 45

Gln?Met?Val?Gln?Asn?Val?Ser?Ala?Tyr?Thr?His?Ala?Ala?Asn?Tyr?Ser

50 55 60

Lys?Phe?Ser?Tyr?Asp?Lys?Val?His?Pro?Ile?Glu?Pro?Gly?Ser?Val?Thr

65 70 75 80

Leu?Val?Ala?Leu?Asp?Gly?Val?Ile?Val?Ser?Glu?Phe?Ala?Leu?Gly?Lys

85 90 95

Arg?Asn?Leu?Tyr?Ala?Asp?Val?Asn?Gly?Thr?Asn?Leu?Pro?Arg?Tyr?Leu

100 105 110

Gln?Glu?Asp?Thr?Thr?Leu?Asp?Thr?Val?Tyr?Asp?Met?Ala?Ser?Leu?Thr

115 120 125

Lys?Leu?Phe?Thr?Thr?Val?Ala?Ala?Leu?Arg?Glu?Leu?Asp?Ala?Gly?Arg

130 135 140

Ile?Ala?Leu?Asn?Val?Thr?Val?Ala?Thr?Tyr?Ile?Pro?Asp?Phe?Ala?Thr

145 150 155 160

Asn?Gly?Lys?Glu?Asn?Ile?Thr?Ile?Leu?Glu?Leu?Phe?Thr?His?Thr?Ser

165 170 175

Gly?Phe?Ala?Ser?Asp?Pro?Ser?Pro?Pro?Leu?Phe?Ser?Ala?Tyr?Tyr?Thr

180 185 190

Thr?Tyr?Asp?Glu?Arg?Ile?Lys?Ala?Ile?Leu?Thr?Gln?Lys?Ile?Ile?Asn

195 200 205

Thr?Pro?Gly?Ser?Thr?Tyr?Leu?Tyr?Leu?Asp?Leu?Asn?Phe?Met?Ser?Leu

210 215 220

Gly?Leu?Val?Ile?Glu?Thr?Val?Thr?Gly?Arg?Ala?Leu?Asp?Asp?Leu?Ile

225 230 235 240

Tyr?Asp?Phe?Thr?Arg?Pro?Leu?Glu?Met?Thr?Ser?Thr?Phe?Phe?Asn?Arg

245 250 255

Gly?Asn?Ile?Glu?Gly?Ser?Thr?Pro?Gln?Ser?Pro?Asn?Tyr?Asp?Arg?Thr

260 265 270

Ala?Val?Gln?Glu?Phe?Gln?Ile?Ala?Ala?Leu?Gly?Pro?Ser?Glu?Pro?Gln

275 280 285

Arg?Pro?Gln?Pro?Val?Arg?Gly?Thr?Val?His?Asp?Glu?Asn?Ala?Trp?Ser

290 295 300

Leu?Asp?Gly?Val?Ser?Gly?His?Ala?Gly?Leu?Phe?Ser?Thr?Val?Arg?Asp

305 310 315 320

Thr?Ala?Thr?Phe?Cys?Gln?Met?Ile?Leu?Asn?Asn?Gly?Thr?Tyr?Ala?Gly

325 330 335

Gln?Arg?Ile?Leu?Ser?Arg?Thr?Ala?Val?Asp?Met?Ile?Phe?Thr?Asn?Phe

340 345 350

Asn?Ala?Arg?Phe?Pro?Gly?Asp?Ala?Arg?Ser?Leu?Gly?Phe?Glu?Leu?Asp

355 360 365

Gln?Tyr?Ser?Thr?Ala?Gly?Pro?Met?Ala?Ser?Leu?Gln?Thr?Ala?Ser?His

370 375 380

Thr?Gly?Phe?Thr?Gly?Thr?Thr?Leu?Val?Met?Asp?Arg?Thr?Tyr?Asn?Ala

385 390 395 400

Phe?Trp?Leu?His?Phe?Ser?Asn?Arg?Val?His?Pro?Ser?Arg?Ala?Trp?Ser

405 410 415

Ser?Asn?Thr?Ile?Val?Arg?Glu?Ala?Ile?Gly?Tyr?Trp?Val?Gly?Lys?Ser

420 425 430

Leu?Gly?Leu?Asp?Val?Ala?Phe?Ala?Leu?Leu

435 440

<210>136

<211>612

<212>PRT

＜213〉aspergillus niger

<400>136

Met?Ala?Ser?Trp?Leu?Leu?Ser?Thr?Leu?Leu?Phe?Leu?Ser?Pro?Ser?Leu

1 5 10 15

Val?Ser?Ala?Lys?Ser?Ala?Ala?Asp?Tyr?Tyr?Val?His?Ser?Leu?Pro?Gly

20 25 30

Ala?Pro?Glu?Gly?Pro?Leu?Leu?Lys?Met?His?Ala?Gly?His?Ile?Glu?Val

35 40 45

Asp?Pro?Gln?Asn?Asn?Gly?Asn?Leu?Phe?Phe?Trp?His?Tyr?Gln?Asn?Arg

50 55 60

His?Ile?Ala?Asn?Arg?Gln?Arg?Thr?Val?Ile?Trp?Leu?Asn?Gly?Gly?Pro

65 70 75 80

Gly?Cys?Ser?Ser?Met?Asp?Gly?Ala?Leu?Met?Glu?Val?Gly?Pro?Tyr?Arg

85 90 95

Leu?Lys?Asp?Asn?Glu?Thr?Leu?Thr?Tyr?Asn?Glu?Gly?Ser?Trp?Asp?Glu

100 105 110

Phe?Ala?Asn?Leu?Leu?Phe?Val?Asp?Gln?Pro?Val?Gly?Thr?Gly?Phe?Ser

115 120 125

Tyr?Val?Asn?Thr?Asp?Ser?Tyr?Leu?His?Glu?Leu?Asp?Glu?Met?Ser?Ala

130 135 140

Gln?Phe?Ile?Val?Phe?Leu?Glu?Glu?Trp?Phe?Arg?Leu?Phe?Pro?Glu?Tyr

145 150 155 160

Glu?Arg?Asp?Asp?Ile?Tyr?Ile?Ala?Gly?Glu?Ser?Tyr?Ala?Gly?Gln?His

165 170 175

Ile?Pro?Tyr?Ile?Ala?Lys?Ala?Ile?Gln?Glu?Arg?Asn?Lys?Asn?Val?Gln

180 185 190

Gly?Lys?Thr?Ile?Ala?Ser?Trp?Asn?Leu?Lys?Gly?Leu?Leu?Ile?Gly?Asn

195 200 205

Gly?Trp?Ile?Ser?Pro?Asn?Glu?Gln?Tyr?Met?Ser?Tyr?Leu?Pro?Tyr?Ala

210 215 220

Tyr?Glu?Glu?Gly?Leu?Ile?Lys?Glu?Gly?Ser?Arg?Thr?Ala?Lys?Glu?Leu

225 230 235 240

Glu?Val?Leu?Gln?Ser?Val?Cys?Lys?Ser?Arg?Leu?Glu?Thr?Gly?Lys?Asn

245 250 255

Lys?Val?His?Leu?Asn?Asp?Cys?Glu?Lys?Val?Met?Asn?Ala?Leu?Leu?Asp

260 265 270

Lys?Thr?Val?Glu?Asp?Asn?Lys?Cys?Leu?Asn?Met?Tyr?Asp?Ile?Arg?Leu

275 280 285

Arg?Asp?Thr?Thr?Asp?Ala?Cys?Gly?Met?Asn?Trp?Pro?Thr?Asp?Leu?Glu

290 295 300

Asp?Val?Lys?Pro?Tyr?Leu?Gln?Arg?Glu?Asp?Val?Val?Lys?Ala?Leu?Asn

305 310 315 320

Ile?Asn?Pro?Glu?Lys?Lys?Ser?Gly?Trp?Val?Glu?Cys?Ser?Gly?Ala?Val

325 330 335

Ser?Ser?Ala?Phe?Asn?Pro?Gln?Lys?Ser?Pro?Pro?Ser?Val?Gln?Leu?Leu

340 345 350

Pro?Gly?Leu?Leu?Glu?Ser?Gly?Leu?Gln?Ile?Leu?Leu?Phe?Ser?Gly?Asp

355 360 365

Lys?Asp?Leu?Ile?Cys?Asn?His?Val?Gly?Thr?Glu?Gln?Leu?Ile?Asn?Asn

370 375 380

Met?Lys?Trp?Asn?Gly?Gly?Thr?Gly?Phe?Glu?Thr?Ser?Pro?Gly?Val?Trp

385 390 395 400

Ala?ProArg?His?Asp?Trp?Ser?Phe?Glu?Gly?Glu?Pro?Ala?Gly?Ile?Tyr

405 410 415

Gln?Tyr?Ala?Arg?Asn?Leu?Thr?Tyr?Val?Leu?Ile?Tyr?Asn?Ala?Ser?His

420 425 430

Met?Val?Pro?Tyr?Asp?Leu?Pro?Arg?Gln?Ser?Arg?Asp?Met?Leu?Asp?Arg

435 440 445

Phe?Met?Asn?Val?Asp?Ile?Ala?Ser?Ile?Gly?Gly?Ser?Pro?Ala?Asp?Ser

450 455 460

Arg?Ile?Asp?Gly?Glu?Lys?Leu?Pro?Gln?Thr?Ser?Val?Gly?Gly?His?Pro

465 470 475 480

Asn?Ser?Thr?Ala?Ala?Glu?Glu?Gln?Glu?Lys?Glu?Arg?Ile?Lys?Glu?Thr

485 490 495

Glu?Trp?Lys?Ala?Tyr?Ala?Lys?Ser?Gly?Glu?Ala?Val?Leu?Leu?Val?Val

500 505 510

Ile?Ile?Gly?Val?Leu?Val?Trp?Gly?Phe?Phe?Ile?Trp?Arg?Ser?Arg?Arg

515 520 525

Arg?His?Gln?Gly?Tyr?Arg?Gly?Val?Trp?His?Lys?Asp?Met?Ser?Gly?Ser

530 535 540

Ser?Val?Leu?Glu?Arg?Phe?His?Asn?Lys?Arg?Thr?Gly?Gly?Ala?Asp?Val

545 550 555 560

Glu?Ala?Gly?Asp?Phe?Asp?Glu?Ala?Glu?Leu?Asp?Asp?Leu?His?Ser?Pro

565 570 575

Asp?Leu?Glu?Arg?Glu?His?Tyr?Ala?Val?Gly?Glu?Asp?Ser?Asp?Glu?Asp

580 585 590

Asp?Ile?Ser?Arg?Gln?His?Ser?Gln?Gln?Ala?Ser?Arg?Ala?Gly?Gly?Ser

595 600 605

His?Asn?Leu?Ser

610

<210>137

<211>531

<212>PRT

＜213〉aspergillus niger

<400>137

Met?Phe?Leu?Ile?Ser?Pro?Ala?Val?Thr?Val?Ala?Ala?Ala?Leu?Leu?Leu

1 5 10 15

Ile?Asn?Gly?Ala?Gly?Ala?Thr?Gln?Ser?Glu?Arg?Ser?Arg?Ala?Ala?Ala

20 25 30

His?Phe?Ser?Lys?Arg?His?Pro?Thr?Tyr?Arg?Ala?Ala?Thr?Arg?Ala?Gln

35 40 45

Ser?Ser?Asn?Thr?Ser?Asp?Tyr?Arg?Phe?Phe?Asn?Asn?Arg?Thr?Lys?Pro

50 55 60

His?Leu?Val?Glu?Ser?Leu?Pro?Asp?Val?His?Phe?Asp?Val?Gly?GLU?Met

65 70 75 80

Tyr?Ser?Gly?Ser?Ile?Pro?Ile?Asp?Asp?Ser?Asn?Asn?Gly?Ser?Arg?Ser

85 90 95

Leu?Phe?Tyr?Ile?Phe?Gln?Pro?Lys?Ile?Gly?Glu?Pro?Ser?Asp?Asp?Leu

100 105 110

Thr?Ile?Tyr?Leu?Asn?Gly?Gly?Pro?Gly?Cys?Ser?Ser?Glu?Gln?Gly?Phe

115 120 125

Phe?Gln?Glu?Asn?Gly?Arg?Phe?Thr?Trp?Gln?Pro?Gly?Thr?Tyr?Ala?Pro

130 135 140

Val?Ile?Asn?Glu?Tyr?Ser?Trp?Val?Asn?Leu?Thr?Asn?Met?Leu?Trp?Val

145 150 155 160

Asp?Gln?Pro?Val?Gly?Thr?Gly?Phe?Ser?Val?Gly?Asn?Val?Thr?Ala?Thr

165 170 175

Asn?Glu?Glu?Glu?Ile?Ala?Ala?Asp?Phe?Leu?Asp?Phe?Phe?Glu?Lys?Phe

180 185 190

Glu?Asp?Leu?Tyr?Gly?Ile?Lys?Asn?Phe?Arg?Ile?Phe?Met?Thr?Gly?Glu

195 200 205

Ser?Tyr?Ala?Gly?Arg?Tyr?Val?Pro?Tyr?Ile?Ser?Ser?Ala?Met?Leu?Asp

210 215 220

Lys?Asn?Asp?Thr?Thr?Arg?Phe?Asn?Leu?Ser?Gly?Ala?Leu?Leu?TYR?Asp

225 230 235 240

Ala?Cys?Ile?Gly?Gln?Trp?Asp?Tyr?Ile?Gln?Ala?Glu?Leu?Pro?Ala?Tyr

245 250 255

Pro?Phe?Val?Lys?Gln?His?Ala?Ser?Leu?Phe?Asn?Phe?Asn?Gln?Ser?Tyr

260 265 270

Met?Asn?Glu?Leu?Glu?Thr?Thr?Tyr?Glu?Glu?Cys?Gly?Tyr?Lys?Ala?Tyr

275 280 285

Phe?Asp?Glu?Tyr?Phe?Ala?Phe?Pro?Pro?Ser?Gly?Ile?Gln?Pro?Pro?Lys

290 295 300

Tyr?Met?Asn?Tyr?Ser?Glu?Cys?Asp?Ile?Tyr?Asn?Met?Ile?Tyr?Tyr?Glu

305 310 315 320

Ala?Tyr?Asn?Pro?Asn?Pro?Cys?Phe?Asn?Pro?Tyr?Arg?Val?Ile?Asp?Glu

325 330 335

Cys?Pro?Leu?Leu?Trp?Asp?Val?Leu?Gly?Trp?Pro?Thr?Asp?Leu?Ala?Tyr

340 345 350

Glu?Pro?Ala?Pro?Thr?Thr?Tyr?Phe?Asn?Arg?Ile?Asp?Val?Lys?Lys?Ala

355 360 365

Leu?His?Ala?Pro?Met?Asp?Val?Glu?Trp?Glu?Leu?Cys?Ser?Tyr?Asp?Leu

370 375 380

Val?Phe?Ala?Gly?Gly?Asp?Ala?Asp?Pro?Gly?Pro?Glu?Gln?Gln?Gly?Asp

385 390 395 400

Asp?Ser?Pro?Asn?Pro?Thr?Glu?Gly?Val?Leu?Pro?Arg?Val?Ile?Glu?Ala

405 410 415

Thr?Asn?Arg?Val?Leu?Ile?Ala?Asn?Gly?Asp?Trp?Asp?Tyr?Leu?Ile?Ile

420 425 430

Thr?Asn?Gly?Thr?Leu?Leu?Ala?Ile?Gln?Asn?Met?Thr?Trp?Asn?Gly?Gln

435 440 445

Leu?Gly?Phe?Gln?Ser?Ala?Pro?Ala?Thr?Pro?Ile?Asp?Ile?Gln?Met?Pro

450 455 460

Asp?Leu?Gln?Trp?Val?Glu?Ile?Phe?Glu?Ala?Gln?Glu?Gly?Tyr?Gly?Gly

465 470 475 480

Leu?Asp?Gly?Pro?Gln?Gly?Val?Met?Gly?Val?Gln?His?Tyr?Glu?Arg?Gly

485 490 495

Leu?Met?Trp?Ala?Glu?Thr?Tyr?Gln?Ser?Gly?His?Lys?Gln?Ala?Gln?Asp

500 505 510

Gln?Gly?Arg?Val?Ser?Tyr?Arg?His?Leu?Gln?Trp?Leu?Leu?Gly?Gln?Val

515 520 525

Glu?Ile?Leu

530

<210>138

<211>531

<212>PRT

＜213〉aspergillus niger

<400>138

Met?Leu?Phe?Arg?Ser?Leu?Leu?Ser?Thr?Ala?Val?Leu?Ala?Val?Ser?Leu

1 5 10 15

Cys?Thr?Asp?Asn?Ala?Ser?Ala?Ala?Lys?His?Gly?Arg?Phe?Gly?Gln?Lys

20 25 30

Ala?Arg?Asp?Ala?Met?Asn?Ile?Ala?Lys?Arg?Ser?Ala?Asn?Ala?Val?Lys

35 40 45

His?Ser?Leu?Lys?Ile?Pro?Val?Glu?Asp?Tyr?Gln?Phe?Leu?Asn?Asn?Lys

50 55 60

Thr?Lys?Pro?Tyr?Arg?Val?Glu?Ser?Leu?Pro?Asp?Val?His?Phe?Asp?Leu

65 70 75 80

Gly?Glu?Met?Tyr?Ser?Gly?Leu?Val?Pro?Ile?Glu?Lys?Gly?Asn?Val?Ser

85 90 95

Arg?Ser?Leu?Phe?Phe?Val?Phe?Gln?Pro?Thr?Ile?Gly?Glu?Pro?Val?Asp

100 105 110

Glu?Ile?Thr?Ile?Trp?Leu?Asn?Gly?Gly?Pro?Gly?Cys?Ser?Ser?Leu?Glu

115 120 125

Ala?Phe?Leu?Gln?Glu?Asn?Gly?Arg?Phe?Val?Trp?Gln?Pro?Gly?Thr?Tyr

130 135 140

Gln?Pro?Val?Glu?Asn?Pro?Tyr?Ser?Trp?Val?Asn?Leu?Thr?Asn?Val?Leu

145 150 155 160

Trp?Val?Asp?Gln?Pro?Val?Gly?Thr?Gly?Phe?Ser?Leu?Gly?Val?Pro?Thr

165 170 175

Ala?Thr?Ser?Glu?Glu?Glu?Ile?Ala?Glu?Asp?Phe?Val?Lys?Phe?Phe?Lys

180 185 190

Asn?Trp?Gln?Gln?Ile?Phe?Gly?Ile?Lys?Asn?Phe?Lys?Ile?Tyr?Val?Thr

195 200 205

Gly?Glu?Ser?Tyr?Ala?Gly?Arg?Tyr?Val?Pro?Tyr?Ile?Ser?Ala?Ala?Phe

210 215 220

Leu?Asp?Gln?Asn?Asp?Thr?Glu?His?Phe?Asn?Leu?Lys?Gly?Ala?Leu?Ala

225 230 235 240

Tyr?Asp?Pro?Cys?Ile?Gly?Gln?Phe?Asp?Tyr?Val?Gln?Glu?Glu?Ala?Pro

245 250 255

Val?Val?Pro?Phe?Val?Gln?Lys?Asn?Asn?Ala?Leu?Phe?Asn?Phe?Asn?Ala

260 265 270

Ser?Phe?Leu?Ala?Glu?Leu?Glu?Ser?Ile?His?Glu?Gln?Cys?Gly?Tyr?Lys

275 280 285

Asp?Phe?Ile?Asp?Gln?Tyr?Leu?Val?Phe?Pro?Ala?Ser?Gly?Val?Gln?Pro

290 295 300

Pro?Lys?Ala?Met?Asn?Trp?Ser?Asp?Pro?Thr?Cys?Asp?Val?Tyr?Asp?Ile

305 310 315 320

Val?Asn?Asn?Ala?Val?Leu?Asp?Pro?Asn?Pro?Cys?Phe?Asn?Pro?Tyr?Glu

325 330 335

Ile?Asn?Glu?Met?Cys?Pro?Ile?Leu?Trp?Asp?Val?Leu?Gly?Phe?Pro?Thr

340 345 350

Glu?Val?Asp?Tyr?Leu?Pro?Ala?Gly?Ala?Ser?Ile?Tyr?Phe?Asp?Arg?Ala

355 360 365

Asp?Val?Lys?Arg?Ala?Met?His?Ala?Pro?Asn?Ile?Thr?Trp?Ser?Glu?Cys

370 375 380

Ser?Val?Glu?Ser?Val?Phe?Val?Gly?Gly?Asp?Gly?Gly?Pro?Glu?Gln?Glu

385 390 395 400

Gly?Asp?Tyr?Ser?Ala?Asn?Pro?Ile?Glu?His?Val?Leu?Pro?Gln?Val?Ile

405 410 415

Glu?Gly?Thr?Asn?Arg?Val?Leu?Ile?Gly?Asn?Gly?Asp?Tyr?Asp?Met?Val

420 425 430

Ile?Leu?Thr?Asn?Gly?Thr?Leu?Leu?Ser?Ile?Gln?Asn?Met?Thr?Trp?Asn

435 440 445

Gly?Lys?Leu?Gly?Phe?Asp?Thr?Ala?Pro?Ser?Thr?Pro?Ile?Asn?Ile?Asp

450 455 460

Ile?Pro?Asp?Leu?Met?Tyr?Asn?Glu?Val?Phe?Ile?Glu?Asn?Gly?Tyr?Asp

465 470 475 480

Pro?Gln?Gly?Gly?Gln?Gly?Val?Met?Gly?Ile?Gln?His?Tyr?Glu?Arg?Gly

485 490 495

Leu?Met?Trp?Ala?Glu?Thr?Phe?Gln?Ser?Gly?His?Met?Gln?Pro?Gln?Phe

500 505 510

Gln?Pro?Arg?Val?Ser?Tyr?Arg?His?Leu?Glu?Trp?Leu?Leu?Gly?Arg?Arg

515 520 525

Asp?Thr?Leu

530

<210>139

<211>492

<212>PRT

＜213〉aspergillus niger

<400>139

Met?Lys?Gly?Ala?Ala?Leu?Ile?Pro?Leu?Ala?Ala?Gly?Ile?Pro?Phe?Ala

1 5 10 15

His?Gly?Leu?Ser?Leu?His?Lys?Arg?Asp?Gly?Pro?Ala?Val?Val?Arg?Met

20 25 30

Pro?Ile?Glu?Arg?Arg?Ser?Ala?Gln?Ser?Leu?Gln?Lys?Arg?Asp?Ser?Thr

35 40 45

Val?Gly?Val?Thr?Leu?Gln?Asn?Trp?Asp?Ala?Thr?Tyr?Tyr?Ala?Val?Asn

50 55 60

Leu?Thr?Leu?Gly?Thr?Pro?Ala?Gln?Lys?Val?Ser?Leu?Ala?Leu?Asp?Thr

65 70 75 80

Gly?Ser?Ser?Asp?Leu?Trp?Val?Asn?Thr?Gly?Asn?Ser?Thr?Tyr?Cys?Ser

85 90 95

Ile?Asp?Asn?Leu?Cys?Thr?Pro?Tyr?Gly?Leu?Tyr?Asn?Ala?Ser?Glu?Ser

100 105 110

Ser?Thr?Val?Lys?Thr?Val?Gly?Thr?His?Leu?Asn?Asp?Thr?Tyr?Ala?Asp

115 120 125

Gly?Thr?Asn?Leu?Tyr?Gly?Pro?Tyr?Val?Thr?Asp?Lys?Leu?Thr?Ile?Gly

130 135 140

Asn?Thr?Thr?Ile?Asp?Asn?Met?Gln?Phe?Gly?Ile?Ala?Glu?Ser?Thr?Thr

145 150 155 160

Ser?Lys?Arg?Gly?Ile?Ala?Gly?Val?Gly?Tyr?Lys?Ile?Ser?Thr?Tyr?Gln

165 170 175

Ala?Glu?His?Asp?Asp?Lys?Val?Tyr?Ala?Asn?Leu?Pro?Gln?Ala?Leu?Val

180 185 190

Asp?Ser?Gly?Ala?Ile?Lys?Ser?Ala?Ala?Tyr?Ser?Ile?Trp?Leu?Asp?Ser

195 200 205

Leu?Glu?Ala?Ser?Thr?Gly?Ser?Leu?Leu?Phe?Gly?Gly?Val?Asn?Thr?Ala

210 215 220

Lys?Tyr?Lys?Gly?Asp?Leu?Gln?Thr?Leu?Pro?Ile?Ile?Pro?Val?Tyr?Gly

225 230 235 240

Lys?Tyr?Tyr?Ser?Leu?Ala?Ile?Ala?Leu?Thr?Glu?Leu?Ser?Val?Ala?Thr

245 250 255

Asp?Ser?Asn?Ser?Ser?Ser?Phe?Thr?Asp?Ser?Leu?Pro?Leu?Ser?Val?Ser

260 265 270

Leu?Asp?Thr?Gly?Thr?Thr?Met?Thr?Ala?Leu?Pro?Ser?Asp?Leu?Val?Asn

275 280 285

Lys?Val?Tyr?Asp?Ala?Leu?Asn?Ala?Thr?Tyr?Asp?Lys?Thr?Tyr?Asp?Met

290 295 300

Ala?Tyr?Ile?Asp?Cys?Asp?Thr?Arg?Glu?Ala?Asp?Tyr?Asn?Val?Thr?Tyr

305 310 315 320

Ser?Phe?Ser?Gly?Ala?Thr?Ile?Thr?Val?Ser?Met?Ser?Glu?Leu?Ile?Ile

325 330 335

Pro?Ala?Thr?Glu?Pro?Gly?Trp?Pro?Asp?Asn?Thr?Cys?Val?Leu?Gly?Leu

340 345 350

Val?Pro?Ser?Gln?Pro?Gly?Val?Asn?Leu?Leu?Gly?Asp?Thr?Phe?Leu?Arg

355 360 365

Ser?Ala?Tyr?Val?Val?Tyr?Asp?Leu?Glu?Asn?Asn?Glu?Ile?Ser?Leu?Ala

370 375 380

Asn?Thr?Asn?Phe?Asn?Pro?Gly?Asp?Asp?Asp?Ile?Leu?Glu?Ile?Gly?Thr

385 390 395 400

Gly?Thr?Ser?Ala?Val?Pro?Gly?Ala?Thr?Pro?Val?Pro?Ser?Ala?Val?Ser

405 410 415

Ser?Ala?Thr?Gly?Asn?Gly?Leu?Ile?Ser?Ser?Gly?Thr?Ala?Val?Pro?Thr

420 425 430

Leu?Ser?Gly?Val?Thr?Ile?Thr?Ala?Thr?Ala?Thr?Ala?Thr?Gly?Ser?Thr

435 440 445

Gly?Thr?Gly?Ser?Ser?Gly?Gly?Ser?Ser?Ala?Glu?Ala?Thr?Ser?Thr?Ser

450 455 460

Ser?Glu?Gly?Ala?Ala?Ala?Gln?Ala?Thr?Ser?Asn?Pro?Met?Asn?Leu?Leu

465 470 475 480

Pro?Gly?Leu?Ala?Gly?Ile?Gly?Leu?Leu?Leu?Ala?Leu

485 490

<210>140

<211>611

<212>PRT

＜213〉aspergillus niger

<400>140

Met?Leu?Ser?Ser?Leu?Leu?Ser?Gln?Gly?Ala?Ala?Val?Ser?Leu?Ala?Val

1 5 10 15

Leu?Ser?Leu?Leu?Pro?Ser?Pro?Val?Ala?Ala?Glu?Ile?Phe?Glu?Lys?Leu

20 25 30

Ser?Gly?Val?Pro?Asn?Gly?Trp?Arg?Tyr?Ala?Asn?Asn?Pro?Gln?Gly?Asn

35 40 45

Glu?Val?Ile?Arg?Leu?Gln?Ile?Ala?Leu?Gln?Gln?His?Asp?Val?Ala?Gly

50 55 60

Phe?Glu?Gln?Ala?Val?Met?Asp?Met?Ser?Thr?Pro?Gly?His?Ala?Asp?Tyr

65 70 75 80

Gly?Lys?His?Phe?Arg?Thr?His?Asp?Glu?Met?Lys?Arg?Met?Leu?Leu?Pro

85 90 95

Ser?Glu?Thr?Ala?Val?Asp?Ser?Val?Arg?Asp?Trp?Leu?Glu?Ser?Ala?Gly

100 105 110

Val?His?Asn?Ile?Gln?Val?Asp?Ala?Asp?Trp?Val?Lys?Phe?His?Thr?Thr

115 120 125

Val?Asn?Lys?Ala?Asn?Ala?Leu?Leu?Asp?Ala?Asp?Phe?Lys?Trp?Tyr?Val

130 135 140

Ser?Asp?Ala?Lys?His?Ile?Arg?Arg?Leu?Arg?Thr?Leu?Gln?Tyr?Ser?Ile

145 150 155 160

Pro?Asp?Ala?Leu?Val?Ser?His?Ile?Asn?Met?Ile?Gln?Pro?Thr?Thr?Arg

165 170 175

Phe?Gly?Gln?Ile?Gln?Pro?Asn?Arg?Ala?Thr?Met?Arg?Ser?Lys?Pro?Lys

180 185 190

His?Ala?Asp?Glu?Thr?Phe?Leu?Thr?Ala?Ala?Thr?Leu?Ala?Gln?Asn?Thr

195 200 205

Ser?His?Cys?Asp?Ser?Ile?Ile?Thr?Pro?His?Cys?Leu?Lys?Gln?Leu?Tyr

210 215 220

Asn?Ile?Gly?Asp?Tyr?Gln?Ala?Asp?Pro?Lys?Ser?Gly?Ser?Lys?Ile?Gly

225 230 235 240

Phe?Ala?Ser?Tyr?Leu?Glu?Glu?Tyr?Ala?Arg?Tyr?Ala?Asp?Leu?Glu?Arg

245 250 255

Phe?Glu?Gln?His?Leu?Ala?Pro?Asn?Ala?Ile?Gly?Gln?Asn?Phe?Ser?Val

260 265 270

Val?Gln?Phe?Asn?Gly?Gly?Leu?Asn?Asp?Gln?Leu?Ser?Ser?Ser?Asp?Ser

275 280 285

Gly?Glu?Ala?Asn?Leu?Asp?Leu?Gln?Tyr?Ile?Leu?Gly?Val?Ser?Ala?Pro

290 295 300

Val?Pro?Ile?Thr?Glu?Tyr?Ser?Thr?Gly?Gly?Arg?Gly?Glu?Leu?Val?Pro

305 310 315 320

Asp?Leu?Ser?Ser?Pro?Asp?Pro?Asn?Asp?Asn?Ser?Asn?Glu?Pro?Tyr?Leu

325 330 335

Asp?Phe?Leu?Gln?Gly?Ile?Leu?Lys?Leu?Asn?Asn?Ser?Asp?Leu?Pro?Gln

340 345 350

Val?Ile?Ser?Thr?Ser?Tyr?Gly?Glu?Asp?Glu?Gln?Thr?Ile?Pro?Val?Pro

355 360 365

Tyr?Ala?Arg?Thr?Val?Cys?Asn?Leu?Tyr?Ala?Gln?Leu?Gly?Ser?Arg?Gly

370 375 380

Val?Ser?Val?Ile?Phe?Ser?Ser?Gly?Asp?Ser?Gly?Val?Gly?Ala?Ala?Cys

385 390 395 400

Leu?Thr?Asn?Asp?Gly?Thr?Asn?Arg?Thr?His?Phe?Pro?Pro?Gln?Phe?Pro

405 410 415

Ala?Ser?Cys?Pro?Trp?Val?Thr?Ser?Val?Gly?Ala?Thr?Ser?Lys?Thr?Ser

420 425 430

Pro?Glu?Gln?Ala?Val?Ser?Phe?Ser?Ser?Gly?Gly?Phe?Ser?Asp?Leu?Trp

435 440 445

Pro?Arg?Pro?Ser?Tyr?Gln?His?Ala?Ala?Val?Gln?Thr?Tyr?Leu?Thr?Lys

450 455 460

His?Leu?Gly?Asn?Lys?Phe?Ser?Gly?Leu?Phe?Asn?Ala?Ser?Gly?Arg?Ala

465 470 475 480

Phe?Pro?Asp?Val?Ser?Ala?Gln?Gly?Val?Asn?Tyr?Ala?Val?Tyr?Asp?Lys

485 490 495

Gly?Met?Leu?Gly?Gln?Phe?Asp?Gly?Thr?Ser?Cys?Ser?Ala?Pro?Thr?Phe

500 505 510

Ser?Gly?Val?Ile?Ala?Leu?Leu?Asn?Asp?Ala?Arg?Leu?Arg?Ala?Gly?Leu

515 520 525

Pro?Val?Met?Gly?Phe?Leu?Asn?Pro?Phe?Leu?Tyr?Gly?Val?Gly?Ser?Glu

530 535 540

Lys?Gly?Ala?Leu?Asn?Asp?Ile?Val?Asn?Gly?Gly?Ser?Val?Gly?Cys?Asp

545 550 555 560

Gly?Arg?Asn?Arg?Phe?Gly?Gly?Thr?Pro?Asn?Gly?Ser?Pro?Val?Val?Pro

565 570 575

Phe?Ala?Ser?Trp?Asn?Ala?Thr?Thr?Gly?Trp?Asp?Pro?Val?Ser?Gly?Leu

580 585 590

Gly?Thr?Pro?Asp?Phe?Ala?Lys?Leu?Lys?Gly?Val?Ala?Leu?Gly?Glu?Glu

595 600 605

Gly?Gly?Asn

610

<210>141

<211>478

<212>PRT

＜213〉aspergillus niger

<400>141

Met?Trp?Leu?Phe?Leu?Val?Cys?Ser?Ile?Leu?Leu?Pro?Leu?Gly?Val?Val

1 5 10 15

Asn?Ala?Gln?Ser?Gln?Tyr?Phe?Asn?Asn?Lys?Thr?Lys?Glu?Phe?Val?Val

20 25 30

Asn?Gly?Ser?Ala?Ile?Pro?Phe?Val?Asp?Phe?Asp?Ile?Gly?Glu?Ser?Tyr

35 40 45

Ala?Gly?Tyr?Leu?Pro?Asn?Thr?Pro?Ser?Gly?Ile?Ser?Ser?Leu?Tyr?Phe

50 55 60

Trp?Phe?Phe?Pro?Ser?Ser?Asp?Pro?Asp?Ala?Ser?Asp?Glu?Ile?Thr?Val

65 70 75 80

Trp?Leu?Asn?Gly?Gly?Pro?Gly?Cys?Ser?Ser?Leu?Ala?Gly?Ile?Met?Leu

85 90 95

Glu?Asn?Gly?Pro?Phe?Leu?Trp?Gln?Pro?Gly?Thr?Tyr?Arg?Pro?Val?Arg

100 105 110

Asn?Pro?Tyr?Ala?Trp?Asn?Asn?Leu?Thr?Asn?Met?Val?Tyr?Ile?Asp?Gln

115 120 125

Pro?Ala?Gly?Thr?Gly?Phe?Ser?Leu?Gly?Pro?Ser?Thr?Val?Val?Ser?Glu

130 135 140

Phe?Asp?Val?Ala?Arg?Gln?Phe?Met?Asp?Phe?Trp?Arg?Arg?Phe?Met?Lys

145 150 155 160

Thr?Phe?Asp?Leu?Gln?Asn?Arg?Lys?Ile?Tyr?Leu?Thr?Gly?Glu?Ser?Tyr

165 170 175

Ala?Gly?Gln?Tyr?Ile?Pro?Tyr?Ile?Ala?Ser?Gln?Met?Leu?Asp?Gln?Asp

180 185 190

Asp?Asp?Glu?Tyr?Phe?Arg?Val?Ala?Gly?Ile?Gln?Ile?Asn?Asp?Pro?Tyr

195 200 205

Ile?Asn?Glu?Leu?Pro?Val?Leu?Gln?Asp?Val?Ala?Thr?Val?Asn?Gln?His

210 215 220

Arg?Ser?Leu?Phe?Pro?Phe?Asn?Asp?Thr?Phe?Met?Ser?Gln?Ile?Thr?Lys

225 230 235 240

Leu?Ser?Asp?Asp?Cys?Gly?Tyr?Thr?Ser?Phe?Leu?Asp?Asp?Ala?Leu?Thr

245 250 255

Phe?Pro?Pro?Arg?Ser?Gln?Phe?Pro?Ser?Val?Pro?Tyr?Asn?Ala?Ser?Cys

260 265 270

Asn?Ile?Trp?Asp?Ile?Ile?Asn?Asn?Ala?Ser?Leu?Ala?Leu?Asn?Pro?Cys

275 280 285

Phe?Asn?Arg?Tyr?His?Ile?Pro?Asp?Ala?Cys?Pro?Thr?Pro?Trp?Asn?Pro

290 295 300

Val?Gly?Gly?Pro?Ile?Val?Gly?Leu?Gly?Pro?Thr?Asn?Tyr?Phe?Asn?Arg

305 310 315 320

Ser?Asp?Val?Gln?Lys?Ala?Ile?Asn?Ala?Tyr?Pro?Thr?Asp?Tyr?Phe?Val

325 330 335

Cys?Lys?Asp?Gly?Ile?Phe?Pro?Thr?Ala?Asn?Gly?Leu?Asp?Thr?Ser?Pro

340 345 350

Pro?Ser?Ser?Leu?Gly?Pro?Leu?Pro?Arg?Val?Ile?Glu?Gln?Thr?Asn?Asn

355 360 365

Thr?Ile?Ile?Ala?His?Gly?Leu?Met?Asp?Phe?Glu?Leu?Leu?Ala?Gln?Gly

370 375 380

Thr?Leu?Ile?Ser?Ile?Gln?Asn?Met?Thr?Trp?Asn?Gly?Lys?Gln?Gly?Phe

385 390 395 400

Glu?Arg?Glu?Pro?Val?Glu?Pro?Leu?Phe?Val?Pro?Tyr?Gly?Gly?Ser?Ser

405 410 415

Gly?Gly?Gly?Val?Leu?Gly?Thr?Ala?His?Thr?Glu?Arg?Gly?Leu?Thr?Phe

420 425 430

Ser?Thr?Val?Phe?Ser?Ser?Gly?His?Glu?Ile?Pro?Glu?Tyr?Ala?Pro?Gly

435 440 445

Ala?Ala?Tyr?Arg?Gln?Leu?Glu?Phe?Leu?Leu?Gly?Arg?Val?Ala?Asn?Leu

450 455 460

Ser?Thr?Ile?Ile?Glu?Gln?Val?Gln?Ile?Thr?Glu?Gln?Asn?Gly

465 470 475

<210>142

<211>210

<212>PRT

＜213〉aspergillus niger

<400>142

Met?Ser?Lys?Leu?Ser?Ala?Ala?Ile?Ser?Lys?Leu?Ser?Leu?Ser?Thr?Ile

1 5 10 15

Ala?Thr?Thr?Leu?Leu?Leu?Leu?Thr?Pro?Pro?Thr?Thr?Ala?Tyr?Phe?Tyr

20 25 30

Lys?Tyr?Pro?Ala?Leu?Phe?Val?Tyr?Lys?Asp?Thr?Asn?Cys?Thr?Asp?Ile

35 40 45

Ser?Phe?Ser?Leu?Val?Tyr?Pro?Ser?Leu?Gly?Asn?Cys?Asn?Gly?Gly?Tyr

50 55 60

Tyr?Asp?Tyr?Ala?Gly?Ser?Phe?Gln?Met?Phe?Asn?Ile?Asp?Ala?Ala?Tyr

65 70 75 80

Thr?Cys?Asn?Gly?Ser?Asp?Ser?Thr?Leu?Met?Phe?Glu?Met?Tyr?Asn?Ser

85 90 95

Ser?Gly?Ser?Asp?Cys?Gly?Asp?Glu?Ser?Asp?Leu?Leu?Phe?Arg?Gln?Pro

100 105 110

Val?Thr?Glu?Glu?Cys?Thr?Val?Ala?Asp?Val?Glu?Ser?Pro?Gly?Pro?Leu

115 120 125

Glu?Met?Pro?Val?Trp?Phe?Glu?Leu?Gly?Ser?Leu?Leu?Gly?Asn?Cys?Gly

130 135 140

Gly?Met?Ala?Gly?Thr?Met?Leu?Phe?Gly?Val?Gly?Ile?Leu?Glu?Gly?Gly

145 150 155 160

Leu?Glu?Thr?Lys?Leu?Tyr?Trp?Lys?Cys?Tyr?Ser?Ser?Arg?Leu?Asn?Thr

165 170 175

Ser?Val?Thr?Val?His?Arg?Leu?Ser?Leu?Ile?Leu?Ser?Met?Gly?Cys?Thr

180 185 190

Ser?Val?Ser?Asp?Ser?Tyr?Asn?Glu?Leu?Ala?Ala?Ala?His?Tyr?Tyr?Glu

195 200 205

Asp?Leu

210

<210>143

<211>608

<212>PRT

＜213〉aspergillus niger

<400>143

Met?Arg?His?Leu?Leu?Ser?Leu?Leu?Val?Leu?Leu?Ile?Ala?Ser?Ala?Ala

1 5 10 15

Leu?Val?Ser?Ala?Val?Pro?Ala?Gly?Ser?Ile?Ile?Thr?Pro?Gln?Pro?Pro

20 25 30

Val?Glu?Pro?Val?His?Leu?Leu?Ser?Ser?Gln?Pro?Ser?Asp?Pro?Arg?Arg

35 40 45

Pro?Trp?Ile?Arg?Leu?Arg?Asp?Trp?Ile?Ile?Glu?Ser?Ile?Trp?Gly?Ile

50 55 60

Glu?Lys?Pro?Ala?Ser?Arg?Arg?Phe?Pro?Leu?Asn?Asp?Ser?Pro?Arg?Asn

65 70 75 80

Arg?Ser?Pro?Pro?Ser?Arg?Ile?Leu?Ala?Arg?Tyr?Gly?Ser?Asp?Val?Val

85 90 95

Leu?Arg?Phe?Ser?Leu?Arg?Asn?His?Asp?Glu?Ala?Glu?Ala?Leu?Ala?Gln

100 105 110

Ala?Ala?Asp?Ile?Leu?Phe?Leu?Asp?Val?Trp?Ala?Ser?Thr?Pro?Ala?Phe

115 120 125

Val?Asp?Ile?Arg?Leu?Ala?Glu?Glu?Val?Thr?Ala?Tyr?Thr?Pro?Leu?Ile

130 135 140

Asp?Asn?Leu?Ala?Glu?Arg?Ile?Tyr?Thr?Thr?Tyr?Pro?Ser?Lys?Lys?Pro

145 150 155 160

Ile?Gly?Leu?Glu?Gly?Gln?Ser?Gly?Phe?Ala?Ser?Ser?Ser?Arg?Pro?Ala

165 170 175

Pro?Lys?Phe?Gly?Asp?Leu?Phe?Phe?His?Glu?Tyr?Gln?Pro?Leu?Ser?Val

180 185 190

Ile?Ile?Pro?Trp?Met?Arg?Leu?Leu?Ala?Ser?Met?Phe?Pro?Ser?His?Val

195 200 205

Arg?Met?Ile?Ser?Val?Gly?Val?Ser?Tyr?Glu?Gly?Arg?Glu?Ile?Pro?Ala

210 215 220

Leu?Arg?Leu?Ser?Ala?Gly?Ser?Ser?Thr?Ala?Ala?Ser?Gly?Pro?Arg?Lys

225 230 235 240

Thr?Ile?Ile?Val?Thr?Gly?Gly?Ser?His?Ala?Arg?Glu?Trp?Ile?Gly?Thr

245 250 255

Ser?Thr?Val?Asn?His?Val?Met?Tyr?Thr?Leu?Ile?Thr?Lys?Tyr?Gly?Lys

260 265 270

Ser?Lys?Ala?Val?Thr?Arg?Leu?Leu?Gln?Asp?Phe?Asp?Trp?Ile?Met?Ile

275 280 285

Pro?Thr?Ile?Asn?Pro?Asp?Gly?Tyr?Val?Tyr?Thr?Trp?Glu?Thr?Asp?Arg

290 295 300

Leu?Trp?Arg?Lys?Asn?Arg?Gln?Arg?Thr?Ser?Leu?Arg?Phe?Cys?Pro?Gly

305 310 315 320

Ile?Asp?Leu?Asp?Arg?Ala?Trp?Gly?Phe?Glu?Trp?Asp?Gly?Gly?Arg?Thr

325 330 335

Arg?Ala?Asn?Pro?Cys?Ser?Glu?Asn?Tyr?Ala?Gly?Asp?Glu?Pro?Phe?Glu

340 345 350

Gly?Met?Glu?Ala?Gln?Gln?Leu?Ala?Gln?Trp?Ala?Leu?Asn?Glu?Thr?Gln

355 360 365

Asn?Asn?Asn?Ala?Asp?Ile?Val?Ser?Phe?Leu?Asp?Leu?His?Ser?Tyr?Ser

370 375 380

Gln?Thr?Ile?Leu?Tyr?Pro?Phe?Ser?Tyr?Ser?Cys?Ser?Ser?Ile?Pro?Pro

385 390 395 400

Thr?Leu?Glu?Ser?Leu?Glu?Glu?Leu?Gly?Leu?Gly?Leu?Ala?Lys?Ala?Ile

405 410 415

Arg?Tyr?Ala?Thr?His?Glu?Ile?Tyr?Asp?Val?Thr?Ser?Ala?Cys?Glu?Gly

420 425 430

Ile?Val?Thr?Ala?Ser?Ala?Ala?Asp?Asn?Asn?Pro?Gly?Arg?Phe?Phe?Pro

435 440 445

Ile?Gly?Gly?Asn?Ser?Gly?Gly?Ser?Ala?Leu?Asp?Trp?Phe?Tyr?His?Gln

450 455 460

Val?His?Ala?Thr?Tyr?Ser?Tyr?Gln?Ile?Lys?Leu?Arg?Asp?Arg?Gly?Ser

465 470 475 480

Tyr?Gly?Phe?Leu?Leu?Pro?Ser?Glu?His?Ile?Ile?Pro?Thr?Gly?Lys?Glu

485 490 495

Ile?Tyr?Asn?Val?Val?Leu?Lys?Leu?Gly?Ser?Phe?Leu?Ile?Gly?Gly?Asp

500 505 510

Ser?Phe?Asp?Val?Asp?Trp?Glu?Ser?Glu?Leu?Phe?Asp?Leu?Ser?Lys?Asp

515 520 525

Glu?Ser?Asp?Leu?Asp?Ser?Arg?Tyr?Ser?Lys?Ser?Asn?Asp?Arg?Ser?Pro

530 535 540

Ala?Tyr?Leu?His?Asn?Ala?Asn?Gly?Pro?Leu?Pro?Asn?Ile?Asp?Glu?Asp

545 550 555 560

Glu?Asp?Lys?Glu?Trp?Val?Met?Val?Glu?Glu?Glu?Asp?Tyr?Thr?Asp?Asp

565 570 575

Asp?Asp?Asp?Asp?Asp?Asp?Asp?Asp?Glu?Glu?Glu?Glu?Glu?Glu?Glu?Glu

580 585 590

Asp?Thr?Tyr?Trp?Ala?Thr?Glu?His?Thr?Tyr?Glu?Phe?Arg?Arg?Arg?Arg

595 600 605

<210>144

<211>416

<212>PRT

＜213〉aspergillus niger

<400>144

Met?Ala?Phe?Leu?Lys?Arg?Ile?Leu?Pro?Leu?Leu?Ala?Leu?Ile?Leu?Pro

1 5 10 15

Ala?Val?Phe?Ser?Ala?Thr?Glu?Gln?Val?Pro?His?Pro?Thr?Ile?Gln?Thr

20 25 30

Ile?Pro?Gly?Lys?Tyr?Ile?Val?Thr?Phe?Lys?Ser?Gly?Ile?Asp?Asn?Ala

35 40 45

Lys?Ile?Glu?Ser?His?Ala?Ala?Trp?Val?Thr?Glu?Leu?His?Arg?Arg?Ser

50 55 60

Leu?Glu?Gly?Arg?Ser?Thr?Thr?Glu?Asp?Asp?Leu?Pro?Ala?Gly?Ile?Glu

65 70 75 80

Arg?Thr?Tyr?Arg?Ile?Ala?Asn?Phe?Ala?Gly?Tyr?Ala?Gly?Ser?Phe?Asp

85 90 95

Glu?Lys?Thr?Ile?Glu?Glu?Ile?Arg?Lys?His?Asp?His?Val?Ala?Tyr?Val

100 105 110

Glu?Gln?Asp?Gln?Val?Trp?Tyr?Leu?Asp?Thr?Leu?Val?Thr?Glu?Arg?Arg

115 120 125

Ala?Pro?Trp?Gly?Leu?Gly?Ser?Ile?Ser?His?Arg?Gly?Gly?Ser?Ser?Thr

130 135 140

Asp?Tyr?Ile?Tyr?Asp?Asp?Ser?Ala?Gly?Glu?Gly?Thr?Tyr?Ala?Tyr?Val

145 150 155 160

Val?Asp?Thr?Gly?Ile?Leu?Ala?Thr?His?Asn?Glu?Phe?Gly?Gly?Arg?Ala

165 170 175

Ser?Leu?Ala?Tyr?Asn?Ala?Ala?Gly?Gly?Glu?His?Val?Asp?Asp?Val?Gly

180 185 190

His?Gly?Thr?His?Val?Ala?Gly?Thr?Ile?Gly?Gly?Lys?Thr?Tyr?Gly?Val

195 200 205

Ser?Lys?Asn?Ala?His?Leu?Leu?Ser?Val?Lys?Val?Phe?Val?Gly?Glu?Ser

210 215 220

Ser?Ser?Thr?Ser?Val?Ile?Leu?Asp?Gly?Phe?Asn?Trp?Ala?Ala?Asn?Asp

225 230 235 240

Ile?Val?Ser?Lys?Asn?Arg?Thr?Ser?Lys?Ala?Ala?Ile?Asn?Met?Ser?Leu

245 250 255

Gly?Gly?Gly?Tyr?Ser?Tyr?Ala?Phe?Asn?Asn?Ala?Val?Glu?Asn?Ala?Phe

260 265 270

Asp?Glu?Gly?Val?Leu?Ser?Cys?Val?Ala?Ala?Gly?Asn?Glu?Asn?Arg?Asp

275 280 285

Ala?Ala?Arg?Thr?Ser?Pro?Ala?Ser?Ala?Pro?Asp?Ala?Ile?Thr?Val?Ala

290 295 300

Ala?Ile?Asn?Arg?Ser?Asn?Ala?Arg?Ala?Ser?Phe?Ser?Asn?Tyr?Gly?Ser

305 310 315 320

Val?Val?Asp?Ile?Phe?Ala?Pro?Gly?Glu?Gln?Val?Leu?Ser?Ala?Trp?Thr

325 330 335

Gly?Ser?Asn?Ser?Ala?Thr?Asn?Thr?Ile?Ser?Gly?Thr?Ser?Met?Ala?Thr

340 345 350

Pro?His?Val?Thr?Gly?Leu?Ile?Leu?Tyr?Leu?Met?Gly?Leu?Arg?Asp?Leu

355 360 365

Ala?Thr?Pro?Ala?Ala?Ala?Thr?Thr?Glu?Leu?Lys?Arg?Leu?Ala?Thr?Arg

370 375 380

Asn?Ala?Val?Thr?Asn?Val?Ala?Gly?Ser?Pro?Asn?Leu?Leu?Ala?Tyr?Asn

385 390 395 400

Gly?Asn?Ser?Gly?Val?Ser?Lys?Gly?Gly?Ser?Asp?Asp?Gly?Asp?Glu?Asp

405 410 415

<210>145

<211>455

<212>PRT

＜213〉aspergillus niger

<400>145

Met?Ile?Thr?Leu?Leu?Ser?Ala?Leu?Phe?Gly?Ser?Val?Val?Tyr?Ala?Ala

1 5 10 15

Thr?Gln?Thr?Val?Leu?Gly?Pro?Glu?Gly?Ala?Asp?Pro?Phe?Thr?Val?Phe

20 25 30

Arg?Ser?Pro?His?Ser?Pro?Ala?Phe?Ser?Ile?Arg?Ile?Gln?Glu?Gln?Asn

35 40 45

Asp?Ser?Ile?Cys?Asp?Ala?Arg?Ser?Pro?Gln?Phe?Thr?Gly?Trp?Leu?Asp

50 55 60

Ile?Gly?Pro?Lys?His?Leu?Phe?Phe?Trp?Tyr?Phe?Glu?Ser?Gln?Asn?Asp

65 70 75 80

Pro?Phe?His?Asp?Pro?Leu?Thr?Leu?Trp?Met?Thr?Gly?Gly?Pro?Gly?Asp

85 90 95

Ser?Ser?Met?Ile?Gly?Leu?Phe?Glu?Glu?Val?Gly?Pro?Cys?Arg?Ile?Asn

100 105 110

Glu?Phe?Gly?Asn?Gly?Thr?Asp?His?Asn?Pro?Trp?Ala?Trp?Thr?Lys?Asn

115 120 125

Ser?Ser?Leu?Leu?Phe?Val?Asp?Gln?Pro?Val?Asp?Val?Gly?Phe?Ser?Tyr

130 135 140

Ile?Asp?Glu?Gly?Tyr?Glu?Leu?Pro?His?Asp?Ser?Arg?Glu?Ala?Ala?Val

145 150 155 160

Asp?Met?His?Arg?Phe?Leu?Arg?Leu?Phe?Ile?Ser?Glu?Ile?Phe?Pro?His

165 170 175

Lys?Gln?Phe?Leu?Pro?Val?His?Leu?Ser?Gly?Glu?Ser?Tyr?Ala?Gly?Arg

180 185 190

Tyr?Ile?Pro?Tyr?Leu?Ala?Thr?Gln?Ile?Leu?Glu?Gln?Asn?Glu?Leu?Tyr

195 200 205

Lys?Asp?Ser?Pro?Arg?Ile?Pro?Leu?Lys?Ser?Cys?Leu?Val?Gly?Asn?Gly

210 215 220

Phe?Met?Ser?Pro?Lys?Asp?Ala?Thr?Phe?Gly?Tyr?Trp?Glu?Thr?Leu?Cys

225 230 235 240

Thr?Thr?Asn?Ser?Gly?Val?Pro?Ser?Pro?Ile?Phe?Asn?Glu?Thr?Arg?Cys

245 250 255

Asp?Ile?Met?Ala?Ala?Asn?Met?Pro?His?Cys?Met?Asp?Leu?Tyr?Asp?Ile

260 265 270

Cys?Ile?Gln?His?Ser?Asp?Pro?Ala?Ile?Cys?His?Ala?Ala?Gln?Ser?Val

275 280 285

Cys?Tyr?Asp?Ser?Val?Val?Gly?Leu?Met?Ala?Lys?Leu?Leu?Leu?Arg?Met

290 295 300

Thr?Thr?Val?Thr?Ala?Pro?Cys?Glu?Ile?Asp?Glu?Met?Cys?Tyr?Ile?Glu

305 310 315 320

Ala?Ala?Leu?Ile?Glu?Arg?Tyr?Leu?Asn?Ser?Pro?Ser?Val?Trp?Glu?Ala

325 330 335

Leu?Ser?Pro?Pro?Gln?Gln?Val?Thr?Glu?Tyr?Lys?Phe?Val?Ala?Thr?Ser

340 345 350

Val?Ile?Asp?Ala?Phe?Ala?Gln?Ser?Ala?Asp?Gly?Met?Val?Ser?Ser?Ser

355 360 365

Lys?Gln?Ile?Ala?Phe?Leu?Leu?Ala?Asn?Asn?Val?Asp?Phe?Leu?Ala?Tyr

370 375 380

Gln?Gly?Asn?Leu?Asp?Leu?Ala?Cys?Asn?Thr?Ala?Gly?Asn?Leu?Arg?Trp

385 390 395 400

Ala?Asn?Ser?Leu?Ser?Trp?Lys?Gly?Gln?Thr?Glu?Phe?Thr?Ala?Lys?Pro

405 410 415

Leu?Leu?Pro?Trp?Glu?Ile?Gln?Val?Ser?Val?Gly?Glu?Gly?Thr?Asp?Glu

420 425 430

Thr?Ser?Arg?Phe?Ala?Phe?Val?Thr?Val?Asp?Asn?Ala?Gly?His?Leu?Leu

435 440 445

Arg?Asp?Ser?Lys?Ile?Ser?Asn

450 455

<210>146

<211>791

<212>PRT

＜213〉aspergillus niger

<400>146

Met?Arg?Phe?Leu?Thr?Tyr?Ser?Leu?Pro?Phe?Ile?Ala?Ser?Ala?Ile?Ser

1 5 10 15

Leu?Phe?Gly?Val?Asn?Val?Gln?Ala?Arg?Ser?Gln?Ala?Pro?Ser?Ala?Ile

20 25 30

Arg?His?Val?Ser?Thr?Leu?Asp?Gln?Pro?Thr?Ile?Lys?Thr?Pro?Ser?Gln

35 40 45

Arg?Val?Asp?His?Leu?Asp?His?Phe?Asp?Ile?Thr?Phe?Asn?Ile?His?Asp

50 55 60

Lys?His?Gln?Arg?Ile?Lys?Leu?Glu?Leu?Glu?Pro?Asn?His?Asp?Ile?Leu

65 70 75 80

Ala?Glu?Asp?Ala?Ser?Val?Gln?Tyr?Leu?Asp?Ala?Asp?Gly?Asn?Val?Arg

85 90 95

Arg?His?Glu?Pro?Ile?Ala?Pro?His?Glu?His?Lys?Val?Phe?Lys?Gly?Arg

100 105 110

Ser?Leu?Leu?Gly?Arg?Gly?Lys?Gly?Met?Trp?Asp?Pro?Val?Gly?Trp?Ala

115 120 125

Arg?Ile?Tyr?Leu?Lys?Gln?Asp?Gly?Ser?Glu?Pro?Leu?Phe?Glu?Gly?Val

130 135 140

Phe?Ser?Ile?Asp?Gly?Asp?Asn?His?His?Val?Gln?Leu?Lys?Ser?Ala?Tyr

145 150 155 160

Met?Glu?Lys?Lys?Arg?Pro?Val?Asp?Val?Asp?Leu?Pro?Asp?Ser?Ala?Thr

165 170 175

Asp?Tyr?Met?Ile?Phe?Tyr?Arg?Asp?Ser?Asp?Met?Val?Arg?Leu?His?Thr

180 185 190

Glu?Leu?Lys?Arg?Ser?Ser?Leu?Gly?Ser?Thr?Ser?Cys?Gln?Ala?Asp?Gln

195 200 205

Leu?Gly?Phe?Asn?Thr?Asn?Pro?Asn?His?Pro?Val?Leu?Gln?Pro?Tyr?Gly

210 215 220

Gln?Ala?Glu?Thr?Asp?Thr?Trp?Gly?Ala?Ile?Ser?Leu?Asn?Ser?Leu?Phe

225 230 235 240

Gly?Leu?Asn?Lys?Arg?Gln?Ser?Asp?Ile?Gly?Ser?Val?Ser?Gly?Asn?Ala

245 250 255

Gly?Gly?Val?Asn?Leu?Ala?Ser?Thr?Ile?Gly?Asp?Thr?Ser?Gly?Cys?Pro

260 265 270

Ser?Thr?Lys?Gln?Val?Ala?Leu?Ile?Gly?Val?Ala?Thr?Asp?Cys?Ala?Phe

275 280 285

Thr?Gly?Ser?Phe?Asn?Asn?Glu?Thr?Ala?Ala?Lys?Glu?Trp?Val?Ile?Ser

290 295 300

Thr?Val?Asn?Ser?Ala?Ser?Asn?Val?Tyr?Glu?Lys?Ser?Phe?Asn?Ile?Thr

305 310 315 320

Ile?Gly?Leu?Arg?Asn?Leu?Thr?Ile?Thr?Asp?Ser?Ser?Cys?Pro?Asp?Asn

325 330 335

Pro?Pro?Ala?Ala?Thr?Ala?Trp?Asn?Met?Pro?Cys?Ser?Ser?Gly?Asn?Leu

340 345 350

Thr?Ser?Arg?Leu?Asp?Leu?Phe?Ser?Lys?Trp?Arg?Gly?Glu?Gln?Ser?Asp

355 360 365

Asp?Asn?Ala?Tyr?Trp?Thr?Leu?Met?Ser?Asp?Cys?Ala?Thr?Gly?Asn?Glu

370 375 380

Val?Gly?Leu?Ser?Trp?Leu?Gly?Gln?Leu?Cys?Asn?Ser?Asp?Ala?Ser?Ser

385 390 395 400

Asp?Gly?Ser?Ser?Thr?Val?Ser?Gly?Thr?Asn?Val?Val?Val?Arg?Ser?Ser

405 410 415

Gly?Ser?Asp?Trp?Gln?Ile?Phe?Ala?His?Glu?Ser?Gly?His?Thr?Phe?Gly

420 425 430

Ala?Val?His?Asp?Cys?Asp?Ser?Gln?Thr?Cys?Ala?Glu?Asp?Leu?Glu?Ala

435 440 445

Ser?Ser?Gln?Cys?Cys?Pro?Leu?Thr?Ser?Ser?Thr?Cys?Asn?Ala?Asn?Gly

450 455 460

Lys?Tyr?Ile?Met?Asn?Pro?Thr?Thr?Gly?Thr?Asp?Ile?Thr?Ala?Phe?Ser

465 470 475 480

Gln?Cys?Thr?Ile?Gly?Asn?Ile?Cys?Ala?Ala?Leu?Gly?Arg?Asn?Ser?Val

485 490 495

Lys?Ser?Ser?Cys?Leu?Ser?Ala?Asn?Arg?Asp?Val?Thr?Thr?Tyr?Thr?Gly

500 505 510

Ser?Gln?Cys?Gly?Asn?Gly?Ile?Val?Glu?Ser?Gly?Glu?Asp?Cys?Asp?Cys

515 520 525

Gly?Gly?Glu?Asp?Gly?Cys?Gly?Asp?Asn?Asn?Cys?Cys?Asp?Ala?Lys?Thr

530 535 540

Cys?Lys?Phe?Lys?Ser?Gly?Ala?Val?Cys?Asp?Asp?Ser?Asn?Asp?Ser?Cys

545 550 555 560

Cys?Ser?Ser?Cys?Gln?Phe?Ser?Ser?Ala?Gly?Thr?Val?Cys?Arg?Ala?Ser

565 570 575

Arg?Gly?Asp?Cys?Asp?Val?Ala?Glu?Thr?Cys?Ser?Gly?Asn?Ser?Ser?Thr

580 585 590

Cys?Pro?Thr?Asp?Ser?Phe?Lys?Lys?Asp?Gly?Thr?Ser?Cys?Gly?Ser?Ser

595 600 605

Gly?Ser?Gly?Leu?Ala?Cys?Ala?Ser?Gly?Gln?Cys?Thr?Ser?Arg?Asp?Tyr

610 615 620

Gln?Cys?Arg?Ser?Val?Met?Gly?Ser?Leu?Leu?His?Ser?Asn?Asp?Thr?Tyr

625 630 635 640

Ala?Cys?Ser?Ser?Phe?Ser?Ser?Ser?Cys?Glu?Leu?Val?Cys?Thr?Ser?Pro

645 650 655

Lys?Ile?Gly?Thr?Cys?Tyr?Ser?Val?Asn?Gln?Asn?Phe?Leu?Asp?Gly?Thr

660 665 670

Pro?Cys?Gly?Ser?Gly?Gly?Tyr?Cys?Ser?Asn?Gly?Asp?Cys?Lys?Gly?Gln

675 680 685

Asn?Val?Glu?Ser?Trp?Ile?Lys?Asn?His?Lys?Gly?Ile?Val?Ile?Gly?Val

690 695 700

Ala?Cys?Ala?Val?Gly?Ala?Leu?Ile?Leu?Leu?Ala?Leu?Met?Thr?Cys?Ile

705 710 715 720

Val?Asn?Arg?Cys?Arg?Arg?Ala?Arg?Ala?Pro?Lys?Pro?Val?Pro?Arg?Pro

725 730 735

Val?Pro?Tyr?Gly?Pro?Trp?Pro?Gly?Ala?Arg?Pro?Pro?Pro?Pro?Pro?Pro

740 745 750

Met?Asn?Gln?Trp?Pro?Ala?Arg?Gly?Tyr?Gln?Gly?Leu?Gly?Asn?Glu?Pro

755 760 765

Pro?Pro?Pro?Tyr?Pro?Gly?Val?Pro?Gly?Gln?Pro?Val?Pro?Gln?His?Met

770 775 780

Pro?Pro?Gln?Gly?Arg?Tyr?Ala

785 790

<210>147

<211>481

<212>PRT

＜213〉aspergillus niger

<400>147

Met?Arg?Phe?Leu?Ser?Ser?Ala?Ala?Leu?Phe?Gly?Leu?Ala?Tyr?Ala?Ser

1 5 10 15

Thr?Gln?Ala?Val?Leu?Gln?Pro?Glu?Glu?Pro?Ser?Asp?Phe?Arg?Thr?Phe

20 25 30

His?Ser?Pro?Tyr?Ser?Pro?His?His?Ser?Ile?Arg?Ile?Arg?Gln?Gln?Asn

35 40 45

Glu?Ser?Ile?Cys?Ala?Ala?His?Ser?Ala?Gln?Tyr?Thr?Gly?Trp?Leu?Asp

50 55 60

Ile?Gly?Arg?Lys?His?Leu?Phe?Phe?Trp?Tyr?Phe?Glu?Ser?Gln?Asn?Asp

65 70 75 80

Pro?Ala?Asn?Asp?Pro?Leu?Thr?Leu?Trp?Met?Thr?Gly?Gly?Pro?Gly?Gly

85 90 95

Ser?Ser?Met?Ile?Gly?Leu?Phe?Glu?Glu?Val?Gly?Pro?Cys?Leu?Ile?Asn

100 105 110

Glu?Tyr?Gly?Asn?Gly?Thr?Tyr?Tyr?Asn?Pro?Trp?Gly?Trp?Ser?Arg?Asn

115 120 125

Ser?Ser?Leu?Leu?Phe?Val?Asp?Gln?Pro?Val?Asp?Val?Gly?Phe?Ser?Tyr

130 135 140

Val?Asp?Glu?Gly?Glu?Asp?Leu?Pro?Gly?Asp?Ser?His?Gln?Ala?Ala?Ile

145 150 155 160

Asp?Met?His?Arg?Phe?Leu?Gln?Leu?Phe?Val?Ser?Glu?Val?Phe?Pro?Gln

165 170 175

Leu?Gln?Thr?Leu?Pro?Val?His?Leu?Ser?Gly?Glu?Ser?Tyr?Ala?Gly?His

180 185 190

Tyr?Val?Pro?Tyr?Leu?Gly?Ser?Gln?Ile?Val?Gln?Gln?Asn?Lys?Leu?Tyr

195 200 205

Pro?Thr?Glu?Pro?Gln?Val?Leu?Leu?His?Ser?Cys?Leu?Val?Gly?Asn?Gly

210 215 220

Tyr?Tyr?Ser?Pro?Arg?Asp?Thr?Thr?Tyr?Gly?Tyr?Trp?Glu?Thr?Leu?Cys

225 230 235 240

Thr?Thr?Asn?Pro?Gly?Val?Pro?Glu?Pro?Val?Phe?Asn?Arg?Thr?Arg?Cys

245 250 255

Asp?Ile?Met?Ala?Ala?Asn?Met?Pro?Arg?Cys?Met?Glu?Val?Ser?Asp?Val

260 265 270

Cys?Val?Arg?Asn?Pro?Asp?Pro?Ala?Ile?Cys?His?Ala?Ala?Ser?Glu?Val

275 280 285

Cys?Tyr?Glu?Gly?Val?Ile?Gly?Trp?Tyr?Asp?Asp?Glu?Ser?Gly?Glu?Gly

290 295 300

Gly?Arg?Asn?Arg?Phe?Asp?Ile?Thr?Ala?Pro?Cys?Ala?Leu?Asp?Gly?Ile

305 310 315 320

Cys?Tyr?Ile?Glu?Ala?Ala?Arg?Ile?Glu?Gln?Tyr?Leu?Asn?Thr?Pro?Ala

325 330 335

Val?Trp?Ala?Ala?Leu?Ser?Pro?Pro?Lys?Glu?Ile?Lys?Glu?Tyr?Lys?Val

340 345 350

Thr?Ser?Asp?Asn?Val?Ser?Arg?Ala?Phe?Asp?Leu?Thr?Ser?Asp?Thr?Met

355 360 365

Thr?Pro?Ala?Ser?Glu?Gln?Val?Ala?Phe?Leu?Leu?Ala?Asn?Gln?Val?His

370 375 380

Phe?Leu?Ala?Tyr?Gln?Gly?Asn?Leu?Asp?Leu?Ala?Cys?Asn?Thr?Ala?Gly

385 390 395 400

Asn?Leu?Arg?Trp?Ala?His?Ser?Leu?Pro?Trp?Arg?Gly?Gln?Val?Glu?Phe

405 410 415

Ala?Ser?Lys?Ala?Leu?Arg?Pro?Trp?Ser?Trp?Val?Asp?Val?Val?Ser?Gly

420 425 430

Lys?Gly?Gly?Val?Ala?Gly?Thr?Thr?Lys?Glu?Glu?Ser?Arg?Phe?Ala?Leu

435 440 445

Val?Thr?Val?Asp?Gly?Ala?Gly?His?Phe?Leu?Pro?Gln?Asp?Arg?Pro?Asp

450 455 460

Ile?Ala?Leu?Asp?Met?Met?Val?Arg?Trp?Ile?Ser?Gly?Ala?Ser?Phe?Thr

465 470 475 480

Glu

<210>148

<211>319

<212>PRT

＜213〉aspergillus niger

<400>148

Met?Thr?Leu?Leu?Leu?Asn?Phe?His?Ala?Leu?Phe?Thr?Val?Ile?Leu?Val

1 5 10 15

Ala?Asn?Leu?Ser?Thr?Arg?Cys?Ser?Ala?Leu?Leu?Ser?Gly?Arg?Asp?Phe

20 25 30

Cys?Ser?Thr?Pro?Ala?Pro?Gly?Glu?Ser?Leu?Arg?Ala?Glu?His?Arg?Arg

35 40 45

Leu?Tyr?Asp?Val?Gln?Ala?Gln?Arg?Asp?Ser?Thr?Ala?Glu?Glu?Ser?Arg

50 55 60

Glu?Val?Val?Pro?Trp?Ile?Glu?Ile?Glu?Thr?Trp?Phe?His?Ile?Val?Ser

65 70 75 80

Ser?Asn?Glu?Ala?Ala?Asn?Thr?Val?Ser?Asp?Asp?Met?Ile?Thr?Ser?Gln

85 90 95

Leu?Ser?Tyr?Leu?Gln?Lys?Ala?Tyr?Glu?Ser?Ala?Thr?Ile?Thr?Tyr?Arg

100 105 110

Leu?Glu?Gly?Ile?Thr?Arg?His?Ile?Asn?Asp?Ser?Trp?Ala?Arg?Asn?Asp

115 120 125

Asp?Glu?Leu?Gly?Met?Lys?Asn?Ala?Leu?Arg?Arg?Gly?Asn?Tyr?Gly?Thr

130 135 140

Leu?Asn?Val?Tyr?Phe?Gln?Thr?Asp?Leu?Gln?Ala?Ser?Ser?Asp?Glu?Asn

145 150 155 160

Ser?Arg?Asp?Tyr?Pro?Asn?Asp?Gly?Asn?Arg?Arg?Thr?Asp?Val?Ser?Asp

165 170 175

Gln?Ser?Ser?Ser?Thr?Val?Leu?Gly?Phe?Cys?Thr?Leu?Pro?Asp?Pro?Ser

180 185 190

Val?Asn?Ser?Ser?Ser?Pro?Arg?Ser?Ser?Tyr?Ile?Lys?Asp?Gly?Cys?Asn

195 200 205

Val?Leu?Ala?Asp?Ile?Met?Pro?Gly?Gly?Ser?Leu?Ala?Gln?Tyr?Asn?Lys

210 215 220

Gly?Gly?Thr?Ala?Val?His?Glu?Val?Gly?His?Trp?Asn?Gly?Leu?Leu?His

225 230 235 240

Thr?Phe?Glu?Gly?Glu?Ser?Cys?Ser?Pro?Asp?Asn?Glu?Gly?Asp?Tyr?Ile

245 250 255

Asp?Asp?Thr?Pro?Glu?Gln?Ser?Glu?Pro?Thr?Ser?Gly?Cys?Pro?Ala?Glu

260 265 270

Lys?Asp?Ser?Cys?Pro?Asp?Leu?Pro?Gly?Leu?Asp?Ala?Ile?His?Asn?Phe

275 280 285

Met?Asp?Tyr?Ser?Ser?Asp?Asp?Cys?Tyr?Glu?Ser?Phe?Thr?Pro?Asp?Gln

290 295 300

Ala?Glu?Arg?Met?Arg?Ser?Met?Trp?Ser?Ala?Met?Arg?Glu?Gly?Lys

305 310 315

<210>149

<211>639

<212>PRT

＜213〉aspergillus niger

<400>149

Met?His?Val?Ser?Leu?Phe?Leu?Leu?Ser?Val?Thr?Ala?Ala?Phe?Ala?Ser

1 5 10 15

Pro?Thr?Pro?His?Asn?Tyr?Val?Val?His?Glu?Arg?Arg?Asp?Ala?Leu?Pro

20 25 30

Ser?Val?Trp?Val?Glu?Glu?Ser?Arg?Leu?Asp?Lys?Gly?Ala?Leu?Leu?Pro

35 40 45

Met?Arg?Ile?Gly?Leu?Thr?Gln?Ser?Asn?Leu?Asp?Arg?Gly?His?Asp?Leu

50 55 60

Leu?Met?Glu?Val?Ser?His?Pro?Gln?Ser?Ser?Arg?Tyr?Gly?Lys?His?Leu

65 70 75 80

Ser?Ser?Glu?Glu?Val?His?Asp?Leu?Phe?Ala?Pro?Ser?Asn?Glu?Ala?Val

85 90 95

Glu?Thr?Val?Arg?Thr?Trp?Ile?Glu?Ser?Ala?Gly?Ile?Ala?Pro?Ser?Arg

100 105 110

Ile?Ser?Gln?Ser?Tyr?Asn?Lys?Gln?Trp?Leu?Gln?Phe?Asp?Ala?His?Ala

115 120 125

Ser?Glu?Val?Glu?Gln?Leu?Leu?Gln?Thr?Glu?Tyr?Tyr?Ile?Tyr?Thr?His

130 135 140

Ala?Asp?Thr?Gly?Ser?Ser?His?Val?Thr?Cys?His?Glu?Tyr?His?Val?Pro

145 150 155 160

Glu?Thr?Ile?Gln?Ser?His?Ile?Asp?Tyr?Ile?Thr?Pro?Gly?Val?Lys?Met

165 170 175

Leu?Glu?Val?Arg?Gly?Thr?Pro?Ser?Lys?Lys?Arg?Asp?Ala?Glu?Lys?Arg

180 185 190

Ser?Leu?Gly?Ser?Leu?Pro?Pro?Ile?Leu?Ala?Pro?Leu?Pro?Ile?Asn?Ile

195 200 205

Thr?Lys?Ile?Phe?Asp?Asp?Pro?Leu?Ala?His?Cys?Asp?Leu?Ala?Val?Thr

210 215 220

Pro?Asp?Cys?Ile?Arg?Ala?Met?Tyr?Asn?Ile?Thr?Lys?Gly?Thr?Thr?Ala

225 230 235 240

Thr?Lys?Gly?Asn?Glu?Leu?Gly?Ile?Phe?Glu?Asp?Leu?Gly?Asp?Ile?Tyr

245 250 255

Ser?Gln?Asp?Asp?Leu?Asn?Leu?Phe?Phe?Ala?Asn?Phe?Ala?Ser?Asp?Ile

260 265 270

Pro?Gln?Gly?Thr?His?Pro?Thr?Leu?Asp?Ser?Ile?Asp?Gly?Ala?Thr?Ala

275 280 285

Pro?Thr?Asp?Val?Thr?Asn?Ala?Gly?Pro?Glu?Ser?Asp?Leu?Asp?Phe?Gln

290 295 300

Ile?Ala?Tyr?Pro?Ile?Ile?Trp?Pro?Gln?Asn?Thr?Ile?Leu?Tyr?Gln?Thr

305 310 315 320

Asp?Asp?Pro?Asn?Tyr?Glu?Asp?Asn?Tyr?Asn?Phe?Lys?Gly?Leu?Leu?Asn

325 330 335

Asn?Phe?Leu?Tyr?Ala?Ile?Asp?Gly?Ser?Tyr?Cys?Asn?Glu?Thr?Ser?Ser

340 345 350

Leu?Asp?Pro?Gln?Tyr?Pro?Asp?Pro?Ser?Pro?Gly?Gly?Tyr?Ser?Ser?Pro

355 360 365

Lys?Gln?Cys?Gly?Val?Tyr?Thr?Pro?Thr?Asn?Val?Ile?Ser?Ile?Ser?Tyr

370 375 380

Gly?Ser?Pro?Glu?Ala?Asp?Leu?Pro?Ile?Ala?Tyr?Gln?Arg?Arg?Gln?Cys

385 390 395 400

His?Glu?Phe?Met?Lys?Leu?Gly?Leu?Gln?Gly?Ile?Ser?Val?Val?Val?Ala

405 410 415

Ser?Gly?Asp?Ser?GlyVal?Ala?Ser?Ser?Thr?Gly?Thr?Cys?Phe?Gly?Asp

420 425 430

Ala?Asp?Asn?Val?Phe?Val?Pro?Asp?Phe?Pro?Ala?Thr?Cys?Pro?Tyr?Leu

435 440 445

Thr?Ala?Val?Gly?Gly?Thr?Tyr?Leu?Pro?Leu?Gly?Ala?Asp?Ala?Ala?Lys

450 455 460

Asp?Gln?Glu?Ile?Ala?Val?Thr?Arg?Phe?Pro?Ser?Gly?Gly?Gly?Phe?Ser

465 470 475 480

Asn?Ile?Tyr?Ala?Arg?Pro?Ser?Tyr?Gln?Asn?His?Ser?Val?Glu?Thr?Tyr

485 490 495

Phe?Ser?Thr?Thr?Ser?Asp?Asp?Leu?Thr?Tyr?Pro?Tyr?Tyr?Ser?Gly?Val

500 505 510

Asn?Tyr?Thr?Asp?Phe?Ser?Asn?Thr?Asp?Gly?Val?Tyr?Asn?Arg?Ile?Gly

515 520 525

Arg?Gly?Tyr?Pro?Asp?Val?Ser?Ala?Ile?Ala?Asp?Asn?Ile?Ile?Ile?Tyr

530 535 540

Asn?Gln?Gly?Glu?Ala?Thr?Leu?Val?Gly?Gly?Thr?Ser?Ala?Ala?Ala?Pro

545 550 555 560

Ala?Phe?Ala?Ala?Met?Leu?Thr?Arg?Ile?Asn?Glu?Glu?Arg?Leu?Ala?Lys

565 570 575

Gly?Lys?Ser?Thr?Val?Gly?Phe?Val?Asn?Pro?Val?Leu?Tyr?Glu?His?Pro

580 585 590

Glu?Ala?Phe?Arg?Asp?Val?Thr?Val?Gly?Ser?Asn?Pro?Gly?Cys?Gly?Thr

595 600 605

Asp?Gly?Phe?Pro?Val?Ala?Gly?Gly?Trp?Asp?Pro?Val?Thr?Gly?Leu?Gly

610 615 620

Thr?Pro?Arg?Phe?Glu?Asp?Leu?Met?Asp?Ile?Phe?Val?Gly?Asp?Asp

625 630 635

<210>150

<211>371

<212>PRT

＜213〉aspergillus niger

<400>150

Met?Ala?Ser?Lys?Thr?Leu?Leu?Leu?Ile?Pro?Ala?Leu?Ala?Thr?Ala?Ala

1 5 10 15

Leu?Gly?Ser?Val?Leu?Asp?Leu?Asp?Ile?Lys?Val?Asp?Leu?Gly?Thr?Pro

20 25 30

Gly?Gly?Pro?Phe?Asp?Leu?Met?Tyr?Asp?Thr?Gly?Ser?Ser?Thr?Leu?Trp

35 40 45

Val?Leu?Asp?Ser?Asn?Cys?Thr?Asp?Asp?Cys?Pro?Asn?Val?Ser?Gly?Tyr

50 55 60

Ser?Arg?His?Gly?Tyr?Asn?Leu?Thr?Ser?Thr?Gly?Val?Asn?Leu?Gly?Val

65 70 75 80

Asn?Asp?Ser?Ile?Ala?Tyr?Ser?Gly?Gly?Thr?Val?Ser?Gly?Phe?Thr?Ala

85 90 95

Thr?Asp?Ile?Leu?Thr?Val?Pro?Asp?Thr?Asn?Val?Ser?Tyr?Arg?Gln?Ser

100 105 110

Phe?Ala?Val?Ile?Thr?Asp?Ser?Thr?Trp?Ala?Ala?Leu?Ala?Ala?Asp?Gly

115 120 125

Phe?Ile?Gly?Leu?Ala?Ser?Ser?Thr?Ile?Ala?Phe?Lys?Asn?Thr?Thr?Thr

130 135 140

Ala?Val?Glu?Gln?Met?Met?Gln?Asp?Gly?Leu?Leu?Asp?Glu?Pro?Arg?Phe

145 150 155 160

Ala?Ile?Tyr?Ala?Gly?Ser?Gly?Glu?Ser?Thr?Val?Thr?Asn?Pro?Asn?Pro

165 170 175

Glu?Asn?Asn?Gly?Val?Phe?Thr?Phe?Gly?Gly?Ser?His?Glu?Glu?Thr?Tyr

180 185 190

Ala?Asp?Gly?Glu?Leu?Gln?Trp?Met?Lys?Met?Leu?Ser?Pro?Phe?Glu?Ile

195 200 205

Tyr?Lys?Thr?Asn?Leu?Leu?Gly?Ile?Gln?Gly?His?Asn?Asn?Ser?Asp?Gly

210 215 220

Gln?Ala?Leu?Ser?Ser?Asp?Val?Leu?Asn?Trp?Tyr?Gly?Gln?Thr?Asn?Leu

225 230 235 240

Phe?Asn?Val?Ala?Gly?Ala?Ser?Ser?Ile?Ser?Ile?Pro?Asn?Asp?Gln?Ile

245 250 255

Glu?Ala?Met?Tyr?Ala?Leu?Thr?Pro?Phe?Ser?Tyr?Ala?Asp?Ile?Ser?Ser

260 265 270

Gly?Tyr?Arg?Pro?Leu?Cys?Ser?Asp?Phe?Asn?Asp?Thr?Trp?Ser?Ile?Ser

275 280 285

Phe?Thr?Met?Gly?Phe?Tyr?Gly?Glu?Gly?Val?Thr?Phe?Asn?Leu?Thr?Gly

290 295 300

Asp?Gln?Leu?Ala?Val?Pro?Gly?Tyr?Gln?Asp?Asp?Asp?His?Cys?Phe?Pro

305 310 315 320

Pro?Phe?Asn?Pro?Trp?Asp?Ser?Tyr?Asn?Thr?Ile?Ile?Gly?Gln?His?Trp

325 330 335

Leu?Ser?Asn?Phe?Tyr?Ala?Val?Phe?Asp?Phe?Gly?Ser?Phe?Asp?Pro?Glu

340 345 350

Thr?Tyr?Asp?Ile?Arg?Val?Gly?Leu?Ala?Pro?Leu?Lys?Lys?Glu?Tyr?Leu

355 360 365

Pro?Ser?Ala

370

<210>151

<211>414

<212>PRT

＜213〉aspergillus niger

<400>151

Met?Phe?Pro?Cys?Ser?Arg?Ile?Trp?Ser?Leu?Leu?Val?Ala?Ala?Ala?Thr

1 5 10 15

Ala?Ser?Ala?Val?Pro?Thr?Ser?Leu?Ala?Thr?Thr?His?Leu?Gln?Ser?Val

20 25 30

Asp?Leu?Leu?Leu?Thr?Arg?Ser?Ser?Tyr?Gly?Phe?Leu?Thr?Asp?Ile?Ala

35 40 45

Leu?Gly?Thr?Pro?Gly?Gln?Ser?Leu?Pro?Tyr?Leu?Val?Asp?Trp?Thr?Trp

50 55 60

Thr?Gly?His?Tyr?Val?Val?Thr?Thr?Leu?Cys?Tyr?Asn?Asp?Pro?Thr?Ala

65 70 75 80

Thr?Tyr?Asp?Cys?Leu?Asn?Val?Asp?Gln?Lys?Ile?Phe?Asn?Gln?Thr?Leu

85 90 95

Ser?Ser?Thr?Phe?Ile?Asn?Gln?Thr?Asp?Gln?Tyr?Gly?Tyr?Leu?Tyr?Trp

100 105 110

Asp?Pro?Asn?His?Phe?Tyr?Phe?Thr?Glu?Pro?Ala?Ala?Ala?Asp?Val?Ala

115 120 125

Thr?Asp?Met?Leu?Arg?Ile?Gly?Pro?Thr?Ala?Val?Asn?Thr?Thr?Ile?Gln

130 135 140

Ala?Ala?Asn?Phe?Val?Phe?Asn?Glu?Thr?Ile?Ser?Ala?Phe?Pro?Phe?Ser

145 150 155 160

Gly?Val?Tyr?Gly?Leu?Ser?Pro?Val?Phe?Gln?Gly?Asp?Asn?Arg?Ser?Val

165 170 175

Gln?Ala?Ser?Phe?Tyr?Gln?Gly?Trp?Arg?Ser?Gly?Ala?Trp?His?Ser?Pro

180 185 190

Ile?Val?Ser?Phe?Ile?Tyr?Cys?His?Asp?Asn?Ala?Thr?Lys?Ala?Val?Cys

195 200 205

Ser?Gly?Tyr?Asp?Gly?Leu?Gln?Thr?Leu?Gly?Gly?Tyr?Asn?Thr?Ser?His

210 215 220

Val?Gln?Gly?Asp?Ile?Thr?Trp?Tyr?Asp?Ile?Ile?Val?Thr?Glu?Ala?Ile

225 230 235 240

Asn?Thr?Leu?Asp?Phe?Val?Tyr?Ala?Pro?Ala?Val?Ile?Asn?Tyr?Trp?Ala

245 250 255

Leu?Asn?Leu?Thr?Arg?Phe?Ser?Ile?Gly?Asp?Glu?Glu?Gln?Glu?Leu?Asn

260 265 270

Lys?Thr?Thr?Thr?Leu?Asp?Gly?Lys?Gln?Ala?Ala?Val?Ala?Ala?Phe?Asp

275 280 285

His?Ala?Ser?Tyr?Gly?Arg?Gly?Ala?Pro?Val?Ser?Val?Tyr?Gly?Tyr?Gln

290 295 300

Arg?Leu?Val?Glu?Leu?Val?Gly?Ala?Lys?Ala?Val?Thr?Leu?Ser?Asp?Pro

305 310 315 320

Pro?Asn?Asn?Gly?Glu?Gln?Gly?Phe?Tyr?Gln?Phe?Asp?Cys?Arg?Asn?Ser

325 330 335

Ser?Leu?Leu?Pro?Pro?Leu?Arg?Tyr?Glu?Phe?Ala?Gly?Ser?Glu?Arg?Ala

340 345 350

Trp?Glu?Ile?Val?Pro?Glu?Asn?Tyr?Val?Glu?Val?Leu?Ala?Asn?Gly?Thr

355 360 365

Asn?Lys?Cys?Thr?Phe?Asn?Val?Arg?Thr?Leu?Gly?Asp?Gly?Ala?Met?Val

370 375 380

Met?Gly?Asn?Phe?Gly?Glu?Thr?Phe?Ala?Ile?Asp?Lys?Tyr?Val?Met?Phe

385 390 395 400

Asp?Phe?Glu?Lys?Leu?Gln?Val?Gly?Ile?Ala?Asp?Phe?Ala?Trp

405 410

<210>152

<211>480

<212>PRT

＜213〉aspergillus niger

<400>152

Met?His?Leu?Pro?Gln?Arg?Leu?Val?Thr?Ala?Ala?Cys?Leu?Cys?Ala?Ser

1 5 10 15

Ala?Thr?Ala?Phe?Ile?Pro?Tyr?Thr?Ile?Lys?Leu?Asp?Thr?Ser?Asp?Asp

20 25 30

Ile?Ser?Ala?Arg?Asp?Ser?Leu?Ala?Arg?Arg?Phe?Leu?Pro?Val?Pro?Lys

35 40 45

Pro?Ser?Asp?Ala?Leu?Ala?Asp?Asp?Ser?Thr?Ser?Ser?Ala?Ser?Asp?Glu

50 55 60

Ser?Leu?Ser?Leu?Asn?Ile?Lys?Arg?Ile?Pro?Val?Arg?Arg?Asp?Asn?Asp

65 70 75 80

Phe?Lys?Ile?Val?Val?Ala?Glu?Thr?Pro?Ser?Trp?Ser?Asn?Thr?Ala?Ala

85 90 95

Leu?Asp?Gln?Asp?Gly?Ser?Asp?Ile?Ser?Tyr?Ile?Ser?Val?Val?Asn?Ile

100 105 110

Gly?Ser?Asp?Glu?Lys?Ser?Met?Tyr?Met?Leu?Leu?Asp?Thr?Gly?Gly?Ser

115 120 125

Asp?Thr?Trp?Val?Phe?Gly?Ser?Asn?Cys?Thr?Ser?Thr?Pro?Cys?Thr?Met

130 135 140

His?Asn?Thr?Phe?Gly?Ser?Asp?Asp?Ser?Ser?Thr?Leu?Glu?Met?Thr?Ser

145 150 155 160

Glu?Glu?Trp?Ser?Val?Gly?Tyr?Gly?Thr?Gly?Ser?Val?Ser?Gly?Leu?Leu

165 170 175

Gly?Lys?Asp?Lys?Leu?Thr?Ile?Ala?Asn?Val?Thr?Val?Arg?Met?Thr?Phe

180 185 190

Gly?Leu?Ala?Ser?Asn?Ala?Ser?Asp?Asn?Phe?Glu?Ser?Tyr?Pro?Met?Asp

195 200 205

Gly?Ile?Leu?Gly?Leu?Gly?Arg?Thr?Asn?Asp?Ser?Ser?Tyr?Asp?Asn?Pro

210 215 220

Thr?Phe?Met?Asp?Ala?Val?Ala?Glu?Ser?Asn?Val?Phe?Lys?Ser?Asn?Ile

225 230 235 240

Val?Gly?Phe?Ala?Leu?Ser?Arg?Ser?Pro?Ala?Lys?Asp?Gly?Thr?Val?Ser

245 250 255

Phe?Gly?Thr?Thr?Asp?Lys?Asp?Lys?Tyr?Thr?Gly?Asp?Ile?Thr?Tyr?Thr

260 265 270

Asp?Thr?Val?Gly?Ser?Asp?Ser?Tyr?Trp?Arg?Ile?Pro?Val?Asp?Asp?Val

275 280 285

Tyr?Val?Gly?Gly?Thr?Ser?Cys?Asp?Phe?Ser?Asn?Lys?Ser?Ala?Ile?Ile

290 295 300

Asp?Thr?Gly?Thr?Ser?Tyr?Ala?Met?Leu?Pro?Ser?Ser?Asp?Ser?Lys?Thr

305 310 315 320

Leu?His?Ser?Leu?Ile?Pro?Gly?Ala?Lys?Ser?Ser?Gly?Ser?Tyr?His?Ile

325 330 335

Ile?Pro?Cys?Asn?Thr?Thr?Thr?Lys?Leu?Gln?Val?Ala?Phe?Ser?Gly?Val

340 345 350

Asn?Tyr?Thr?Ile?Ser?Pro?Lys?Asp?Tyr?Val?Gly?Ala?Thr?Ser?Gly?Ser

355 360 365

Gly?Cys?Val?Ser?Asn?Ile?Ile?Ser?Tyr?Asp?Leu?Phe?Gly?Asp?Asp?Ile

370 375 380

Trp?Leu?Leu?Gly?Asp?Thr?Phe?Leu?Lys?Asn?Val?Tyr?Ala?Val?Phe?Asp

385 390 395 400

Tyr?Asp?Glu?Leu?Arg?Val?Gly?Phe?Ala?Glu?Arg?Ser?Ser?Asn?Thr?Thr

405 410 415

Ser?Ala?Ser?Asn?Ser?Thr?Ser?Ser?Gly?Thr?Ser?Ser?Thr?Ser?Gly?Ser

420 425 430

Thr?Thr?Thr?Gly?Ser?Ser?Thr?Thr?Thr?Thr?Ser?Ser?Ala?Ser?Ser?Ser

435 440 445

Ser?Ser?Ser?Asp?Ala?Glu?Ser?Gly?Ser?Ser?Met?Thr?Ile?Pro?Ala?Pro

450 455 460

Gln?Tyr?Phe?Phe?Ser?Ala?Leu?Ala?Ile?Ala?Ser?Phe?Met?Leu?Trp?Leu

465 470 475 480

<210>153

<211>466

<212>PRT

＜213〉aspergillus niger

<400>153

Met?Thr?Ser?Ser?Thr?Leu?Arg?Leu?Ala?Val?Ala?Leu?Ala?Leu?Ser?Thr

1 5 10 15

Cys?Ser?Ser?Ala?Leu?Ser?Ser?Gln?Arg?Asp?Asp?Ser?Leu?Val?Val?Pro

20 25 30

Phe?Pro?Phe?Gly?Asn?Leu?Glu?Asp?Val?His?Ile?Ala?Lys?Arg?Asp?Ser

35 40 45

Ser?Lys?Thr?Val?Glu?Ala?Pro?Leu?Val?Ile?Tyr?Gly?Asp?Ser?Tyr?Trp

50 55 60

Met?Asn?Ala?Ser?Ile?Gly?Thr?Pro?Ala?Gln?Ser?Leu?Ser?Phe?Leu?Leu

65 70 75 80

Asp?Leu?Thr?Arg?Ser?Arg?Val?Glu?Pro?Ala?Tyr?Thr?Leu?Asp?Glu?Asn

85 90 95

Tyr?Glu?Cys?Ser?Asp?Asp?Glu?Leu?Cys?Ser?Glu?Phe?Gly?Phe?Tyr?Lys

100 105 110

Pro?Thr?Asp?Ser?Ser?Thr?Tyr?Gln?His?Leu?Thr?Tyr?Thr?Gln?Arg?His

115 120 125

Asp?Ala?Gly?Val?Asp?Tyr?Ser?Tyr?Leu?Asp?Thr?Ile?Thr?Leu?Gly?Asp

130 135 140

His?Ala?Thr?Asp?Asn?Val?Pro?Leu?Asp?Met?Tyr?Leu?Leu?Ser?Tyr?Ile

145 150 155 160

Ser?Tyr?Ser?Ser?Leu?Gly?Leu?Ser?Ser?Val?Asn?Thr?Ser?Phe?Pro?Tyr

165 170 175

Ile?Leu?Val?Asp?Arg?Gly?Leu?Thr?Thr?Ser?Pro?Ser?Phe?Ser?Leu?Ile

180 185 190

Gly?Asp?Asn?Gly?Asn?Thr?Thr?Thr?Pro?Ser?Ile?Ile?Phe?Gly?Gly?Ile

195 200 205

Asn?Thr?Ser?Lys?Phe?Asn?Gly?Pro?Leu?Gln?Ala?Phe?Ser?Phe?Ala?Asp

210 215 220

His?Ser?Ile?Thr?Asn?Asn?Pro?Phe?Val?Thr?Val?Glu?Ala?Asp?Ser?Leu

225 230 235 240

Gln?Leu?Thr?Thr?Asn?Thr?Asn?Asp?Asn?Ser?Thr?Tyr?Pro?Ile?Pro?Ser

245 250 255

Ser?Thr?Pro?Met?Met?Leu?Arg?Thr?Glu?Glu?Leu?Ile?Thr?Tyr?Leu?Pro

260 265 270

Asn?Ser?Thr?Val?Gln?Ser?Leu?Tyr?Thr?Asp?Leu?Asn?Ile?Thr?Met?Asp

275 280 285

Gly?Val?Ile?Ser?Thr?Ser?Arg?Phe?Tyr?Gly?Val?Leu?Pro?Cys?Ala?Arg

290 295 300

Gln?Glu?Thr?Glu?Ser?His?Thr?Ile?Ser?Leu?Ala?Ile?Gly?Asn?Met?Thr

305 310 315 320

Phe?Ser?Val?Ser?Trp?Asp?Glu?Leu?Phe?Val?Pro?Trp?Thr?Arg?Asp?Gly

325 330 335

Leu?Cys?Lys?Phe?Gly?Ile?Gln?Ala?Gln?Asp?Ser?Asp?Tyr?Lys?Thr?Arg

340 345 350

Ala?Glu?Leu?Gly?Val?Pro?Phe?Leu?Arg?Arg?Met?Tyr?Val?Ala?Val?Asp

355 360 365

Tyr?Asn?Asn?Gln?Phe?Val?Gly?Val?Ala?Thr?Leu?Lys?Asp?Asp?Asp?Asp

370 375 380

Gln?Asn?Gly?Gly?Glu?Asp?Glu?Ile?Val?Glu?Ile?Gly?Thr?Gly?Thr?Ala

385 390 395 400

Leu?Pro?Ser?Ala?Val?Gly?Asp?Trp?Pro?Ala?Ser?Val?Thr?Ala?Tyr?Thr

405 410 415

Pro?Ala?Ala?Ser?Thr?Gly?Thr?Ala?Ala?Ala?Thr?Leu?Thr?Phe?Thr?Thr

420 425 430

Ala?Thr?Ser?Ser?Gly?Gly?Gly?Val?Val?Pro?Thr?Gly?Leu?Ser?Glu?Leu

435 440 445

Gly?Arg?Ala?Phe?Leu?Val?Pro?Gly?Val?Leu?Gly?Met?Ala?Val?Leu?Gln

450 455 460

Ala?Val

465

<210>154

<211>543

<212>PRT

＜213〉aspergillus niger

<400>154

Met?Met?Arg?Pro?Ile?Leu?Leu?Pro?Leu?Leu?Gly?Val?Phe?Leu?Gln?Thr

1 5 10 15

Ser?Ser?Ala?Ser?Asn?Pro?Tyr?Val?Met?Ser?Trp?Ser?Ser?Gln?Ala?Tyr

20 25 30

Gly?Pro?Asp?Gly?Pro?Trp?Gln?Ala?Val?Ser?Ile?Asp?Val?Gly?Ser?Asn

35 40 45

Gln?Gln?Thr?Val?Asp?Leu?Tyr?Pro?Gly?Ala?Asn?Tyr?Ala?Ser?Thr?Ile

50 55 60

Leu?Met?Ser?Thr?Leu?Cys?Thr?Asn?Lys?Thr?Leu?Ser?Ser?Thr?Cys?Tyr

65 70 75 80

Ala?Ala?Glu?Ala?Gly?Thr?Phe?Asn?Gln?Asn?Thr?Ser?Thr?Thr?Ala?Tyr

85 90 95

Thr?Thr?Ala?Ser?Ser?Trp?Glu?Thr?Thr?Tyr?Trp?Ala?Val?Glu?Gly?Gly

100 105 110

Ser?Gln?Glu?Ala?Val?Leu?Gly?Asp?Glu?Val?Thr?Leu?Gly?Ser?Phe?Val

115 120 125

Val?Pro?Asn?Val?Ser?Phe?Glu?Ala?Ile?Tyr?Gln?Thr?Tyr?Gln?Thr?Tyr

130 135 140

Pro?Asn?Gly?Ile?Ala?Tyr?Pro?Val?Ser?Val?Gly?Ser?Leu?Ala?Leu?Gly

145 150 155 160

Gly?Pro?Tyr?Leu?Ser?Asp?Thr?Val?Ser?Asn?Ser?Thr?Val?Leu?Asn?Met

165 170 175

Ile?Ala?Gly?Trp?Leu?Tyr?Ser?Ser?Asn?Asp?Ile?Pro?Ser?Tyr?Ser?Tyr

180 185 190

Gly?Met?His?Ile?Gly?Ser?Val?Asp?Pro?Lys?Ile?Pro?Gly?Ser?Leu?Ile

195 200 205

Leu?Gly?Gly?Tyr?Asp?Lys?Ser?Arg?Val?Ile?Gly?Asp?Val?Ser?Ala?Gln

210 215 220

Gly?Val?Val?Ser?Ser?Ser?Gly?Leu?Leu?Glu?Leu?Glu?Leu?Lys?Asp?Ile

225 230 235 240

Gly?Leu?Gly?Val?Ala?Ala?Gly?Ser?Ser?Pro?Phe?Ser?Phe?Asn?Asn?Glu

245 250 255

Ser?Gly?Leu?Phe?Leu?Gln?Ser?Ser?Gly?Ser?Val?Gln?Ala?Lys?Thr?Val

260 265 270

Gln?Ile?Asp?Pro?Thr?Lys?Pro?Tyr?Met?Tyr?Leu?Pro?Gln?Ala?Thr?Cys

275 280 285

Asp?Ala?Ile?Thr?Ser?Thr?Met?Pro?Ile?Ser?Phe?Asn?Ser?Ser?Leu?Gly

290 295 300

Leu?Tyr?Phe?Trp?Asp?Thr?Thr?Ser?Asp?Asp?Tyr?Leu?Asn?Ile?Thr?Ser

305 310 315 320

Ser?Ala?Ala?Tyr?Leu?Ser?Phe?Val?Phe?Asn?Met?Asn?Gly?Val?Asn?Asn

325 330 335

Lys?Asn?Ile?Thr?Ile?Lys?Ile?Pro?Phe?Ser?Gln?Leu?Asn?Leu?Thr?Leu

340 345 350

Gln?Glu?Pro?Leu?Val?Asp?Gln?Asn?Val?Thr?Tyr?Phe?Pro?Cys?Phe?Leu

355 360 365

Thr?Thr?Ser?Thr?Pro?Val?Leu?Gly?Arg?Ala?Phe?Leu?Gln?Ser?Ala?Phe

370 375 380

Val?Gly?Val?Asn?Trp?Phe?Asn?Gly?Asn?Asn?Ser?Gly?Thr?Trp?Phe?Leu

385 390 395 400

Ala?Gln?Ala?Pro?Gly?Pro?Gly?Tyr?Ala?Ser?Glu?Asp?Ile?Thr?Arg?Ile

405 410 415

Ala?Val?Ser?Asp?Thr?Ser?Leu?Ser?Ala?Ser?Asn?Gly?Thr?Trp?Glu?Glu

420 425 430

Thr?Trp?Ala?Thr?Tyr?Trp?Gly?Ile?Lys?Thr?Ser?Asp?Asn?Ser?Ser?Ser

435 440 445

Ser?Lys?Ser?Gly?Leu?Ser?Ser?Gly?Ala?Lys?Ile?Gly?Ile?Gly?Val?Gly

450 455 460

Val?Gly?Val?Gly?Gly?Ala?Val?Leu?Ile?Ala?Ala?Gly?Ile?Ala?Ile?Ala

465 470 475 480

Phe?Cys?Leu?Arg?Arg?Arg?Arg?Gly?Ala?Ser?Gln?Glu?Ala?Ala?Gly?Glu

485 490 495

Gln?Arg?Arg?Ser?Met?Phe?Arg?Gly?Phe?Ala?Glu?Leu?Pro?Gly?Gly?Ala

500 505 510

His?Ser?Glu?Pro?Ala?Lys?Glu?Leu?Asp?Thr?Lys?Met?His?Lys?Pro?Pro

515 520 525

Gln?Glu?Met?Met?Ala?Ser?Gln?Glu?Val?Glu?Arg?Tyr?Glu?Leu?Gly

530 535 540

<210>155

<211>844

<212>PRT

＜213〉aspergillus niger

<400>155

Met?Arg?Leu?Thr?Gly?Gly?Val?Ala?Ala?Ala?Leu?Gly?Leu?Cys?Ala?Ala

1 5 10 15

Ala?Ser?Ala?Ser?Leu?His?Pro?His?Arg?Ser?Tyr?Glu?Thr?His?Asp?Tyr

20 25 30

Phe?Ala?Leu?His?Leu?Asp?Glu?Ser?Thr?Ser?Pro?Ala?Asp?Val?Ala?Gln

35 40 45

Arg?Leu?Gly?Ala?Arg?His?Glu?Gly?Pro?Val?Gly?Glu?Leu?Pro?Ser?His

50 55 60

His?Thr?Phe?Ser?Ile?Pro?Arg?Glu?Asn?Ser?Asp?Asp?Val?His?Ala?Leu

65 70 75 80

Leu?Asp?Gln?Leu?Arg?Asp?Arg?Arg?Arg?Leu?Arg?Arg?Arg?Ser?Gly?Asp

85 90 95

Asp?Ala?Ala?Val?Leu?Pro?Ser?Leu?Val?Gly?Arg?Asp?Glu?Gly?Leu?Gly

100 105 110

Gly?Ile?Leu?Trp?Ser?Glu?Lys?Leu?Ala?Pro?Gln?Arg?Lys?Leu?His?Lys

115 120 125

Arg?Val?Pro?Pro?Thr?Gly?Tyr?Ala?Ala?Arg?Ser?Pro?Val?Asn?Thr?Gln

130 135 140

Asn?Asp?Pro?Gln?Ala?Leu?Ala?Ala?Gln?Lys?Arg?Ile?Ala?Ser?Glu?Leu

145 150 155 160

Gly?Ile?Ala?Asp?Pro?Ile?Phe?Gly?Glu?Gln?Trp?His?Leu?Tyr?Asn?Thr

165 170 175

Val?Gln?Leu?Gly?His?Asp?Leu?Asn?Val?Thr?Gly?Ile?Trp?Leu?Glu?Gly

180 185 190

Val?Thr?Gly?Gln?Gly?Val?Thr?Thr?Ala?Ile?Val?Asp?Asp?Gly?Leu?Asp

195 200 205

Met?Tyr?Ser?Asn?Asp?Leu?Arg?Pro?Asn?Tyr?Phe?Ala?Ala?Gly?Ser?Tyr

210 215 220

Asp?Tyr?Asn?Asp?Lys?Val?Pro?Glu?Pro?Arg?Pro?Arg?Leu?Ser?Asp?Asp

225 230 235 240

Arg?His?Gly?Thr?Arg?Cys?Ala?Gly?Glu?Ile?Gly?Ala?Ala?Lys?Asn?Asp

245 250 255

Val?Cys?Gly?Val?Gly?Val?Ala?Tyr?Asp?Ser?Arg?Ile?Ala?Gly?Ile?Arg

260 265 270

Ile?Leu?Ser?Ala?Pro?Ile?Asp?Asp?Thr?Asp?Glu?Ala?Ala?Ala?Ile?Asn

275 280 285

Tyr?Ala?Tyr?Gln?Glu?Asn?Asp?Ile?Tyr?Ser?Cys?Ser?Trp?Gly?Pro?Tyr

290 295 300

Asp?Asp?Gly?Ala?Thr?Met?Glu?Ala?Pro?Gly?Thr?Leu?Ile?Lys?Arg?Ala

305 310 315 320

Met?Val?Asn?Gly?Ile?Gln?Asn?Gly?Arg?Gly?Gly?Lys?Gly?Ser?Val?Phe

325 330 335

Val?Phe?Ala?Ala?Gly?Asn?Gly?Ala?Ile?His?Asp?Asp?Asn?Cys?Asn?Phe

340 345 350

Asp?Gly?Tyr?Thr?Asn?Ser?Ile?Tyr?Ser?Ile?Thr?Val?Gly?Ala?Ile?Asp

355 360 365

Arg?Glu?Gly?Asn?His?Pro?Pro?Tyr?Ser?Glu?Ser?Cys?Ser?Ala?Gln?Leu

370 375 380

Val?Val?Ala?Tyr?Ser?Ser?Gly?Ala?Ser?Asp?Ala?Ile?His?Thr?Thr?Asp

385 390 395 400

Val?Gly?Thr?Asp?Lys?Cys?Ser?Thr?Thr?His?Gly?Gly?Thr?Ser?Ala?Ala

405 410 415

Gly?Pro?Leu?Ala?Ala?Gly?Thr?Val?Ala?Leu?Ala?Leu?Ser?Val?Arg?Pro

420 425 430

Glu?Leu?Thr?Trp?Arg?Asp?Val?Gln?Tyr?Leu?Met?Ile?Glu?Ala?Ala?Val

435 440 445

Pro?Val?His?Glu?Asp?Asp?Gly?Ser?Trp?Gln?Asp?Thr?Lys?Asn?Gly?Lys

450 455 460

Lys?Phe?Ser?His?Asp?Trp?Gly?Tyr?Gly?Lys?Val?Asp?Thr?Tyr?Thr?Leu

465 470 475 480

Val?Lys?Arg?Ala?Glu?Thr?Trp?Asp?Leu?Val?Lys?Pro?Gln?Ala?Trp?Leu

485 490 495

His?Ser?Pro?Trp?Gln?Arg?Val?Glu?His?Glu?Ile?Pro?Gln?Gly?Glu?Gln

500 505 510

Gly?Leu?Ala?Ser?Ser?Tyr?Glu?Val?Thr?Glu?Asp?Met?Leu?Lys?Gly?Ala

515 520 525

Asn?Leu?Glu?Arg?Leu?Glu?His?Val?Thr?Val?Thr?Met?Asn?Val?Asn?His

530 535 540

Thr?Arg?Arg?Gly?Asp?Leu?Ser?Val?Glu?Leu?Arg?Ser?Pro?Asp?Gly?Arg

545 550 555 560

Val?Ser?His?Leu?Ser?Thr?Pro?Arg?Arg?Pro?Asp?Asn?Gln?Glu?Val?Gly

565 570 575

Tyr?Val?Asp?Trp?Thr?Phe?Met?Ser?Val?Ala?His?Trp?Gly?Glu?Ser?Gly

580 585 590

Ile?Gly?Lys?Trp?Thr?Val?Ile?Val?Lys?Asp?Thr?Asn?Val?Asn?Glu?His

595 600 605

Thr?Gly?Gln?Phe?Ile?Asp?Trp?Arg?Leu?Asn?Leu?Trp?Gly?Glu?Ala?Ile

610 615 620

Asp?Gly?Ala?Glu?Gln?Pro?Leu?His?Pro?Met?Pro?Thr?Glu?His?Asp?Asp

625 630 635 640

Asp?His?Ser?Tyr?Glu?Glu?Gly?Asn?Val?Ala?Thr?Thr?Ser?Ile?Ser?Ala

645 650 655

Val?Pro?Thr?Lys?Thr?Glu?Leu?Pro?Asp?Lys?Pro?Thr?Gly?Gly?Val?Asp

660 665 670

Arg?Pro?Val?Asn?Val?Lys?Pro?Thr?Thr?Ser?Ala?Met?Pro?Thr?Gly?Ser

675 680 685

Leu?Thr?Glu?Pro?Ile?Asp?Asp?Glu?Glu?Leu?Gln?Lys?Thr?Pro?Ser?Thr

690 695 700

Glu?Ala?Ser?Ser?Thr?Pro?Ser?Pro?Ser?Pro?Thr?Thr?Ala?Ser?Asp?Ser

705 710 715 720

Ile?Leu?Pro?Ser?Phe?Phe?Pro?Thr?Phe?Gly?Ala?Ser?Lys?Arg?Thr?Gln

725 730 735

Val?Trp?Ile?Tyr?Ala?Ala?Ile?Gly?Ser?Ile?Ile?Val?Phe?Cys?Ile?Gly

740 745 750

Leu?Gly?Val?Tyr?Phe?His?Val?Gln?Arg?Arg?Lys?Arg?Ile?Arg?Asp?Asp

755 760 765

Ser?Arg?Asp?Asp?Tyr?Asp?Phe?Glu?Met?Ile?Glu?Asp?Glu?Asp?Glu?Leu

770 775 780

Gln?Ala?Met?Asn?Gly?Arg?Ser?Asn?Arg?Ser?Arg?Arg?Arg?Gly?Gly?Glu

785 790 795 800

Leu?Tyr?Asn?Ala?Phe?Ala?Gly?Glu?Ser?Asp?Glu?Glu?Pro?Leu?Phe?Ser

805 810 815

AspGlu?Asp?Asp?Glu?Pro?Tyr?Arg?Asp?Arg?Gly?Ile?Ser?Gly?Glu?Gln

820 825 830

Glu?Arg?Glu?Gly?Ala?Asp?Gly?Glu?His?Ser?Arg?Arg

835 840

<210>156

<211>149

<212>PRT

＜213〉aspergillus niger

<400>156

Met?Lys?Thr?Phe?Ser?Thr?Val?Thr?Ser?Leu?Leu?Ala?Leu?Phe?Ser?Ser

1 5 10 15

Ala?Leu?Ala?Ala?Pro?Val?Asp?Ser?Ala?Glu?Ala?Ala?Gly?Thr?Thr?Val

20 25 30

Ser?Val?Ser?Tyr?Asp?Thr?Ala?Tyr?Asp?Val?Ser?Gly?Ala?Ser?Leu?Thr

35 40 45

Thr?Val?Ser?Cys?Ser?Asp?Gly?Ala?Asn?Gly?Leu?Ile?Asn?Lys?Gly?Tyr

50 55 60

Ser?Asn?Phe?Gly?Ser?Leu?Pro?Gly?Phe?Pro?Lys?Ile?Gly?Gly?Ala?Pro

65 70 75 80

Thr?Ile?Ala?Gly?Trp?Asn?Ser?Pro?Asn?Cys?Gly?Lys?Cys?Tyr?Ala?Leu

85 90 95

Thr?Tyr?Asn?Gly?Gln?Thr?Val?Asn?Ile?Leu?Ala?Ile?Asp?Ser?Ala?Pro

100 105 110

Gly?Gly?Phe?Asn?Ile?Ala?Leu?Glu?Ala?Met?Asn?Thr?Leu?Thr?Asn?Asn

115 120 125

Gln?Ala?Gln?Gln?Leu?Gly?Arg?Ile?Glu?Ala?Thr?Tyr?Thr?Glu?Val?Asp

130 135 140

Val?Ser?Leu?Cys?Ala

145

<210>157

<211>296

<212>PRT

＜213〉aspergillus niger

<400>157

Met?Ala?Gln?Ile?Phe?Trp?Leu?Ser?Leu?Phe?Leu?Leu?Val?Ser?Trp?Val

1 5 10 15

Arg?Ala?Glu?Ser?Asn?Arg?Thr?Glu?Val?Asp?Leu?Ile?Phe?Pro?Arg?Asn

20 25 30

Asp?Thr?Phe?Ala?Pro?Met?Pro?Leu?Met?Pro?Val?Val?Phe?Ala?Val?Gln

35 40 45

Ala?Pro?Ser?Val?Ala?His?Lys?Val?Asn?Thr?Tyr?Ile?Glu?Tyr?Gly?Tyr

50 55 60

Tyr?Pro?Val?Gly?Arg?Pro?Asn?Glu?Thr?Val?Ile?Gly?Gln?Thr?Asp?His

65 70 75 80

Val?Ser?Asp?Ser?Thr?Asn?Glu?Thr?Thr?Tyr?Phe?Ser?Val?Ser?Gly?Ile

85 90 95

Gly?Arg?Thr?Phe?Asn?Thr?Thr?Gly?Ser?Trp?Glu?Leu?Phe?Trp?Arg?Leu

100 105 110

Arg?Trp?Thr?Asn?Cys?Ser?Ile?Ser?Glu?Asp?Ser?Arg?Tyr?Tyr?Asn?Gln

115 120 125

Ser?Tyr?Pro?Trp?Ile?Ser?Ser?Pro?Tyr?Ile?Asp?Gly?Ser?Leu?Asn?Ile

130 135 140

Asp?Lys?Val?Tyr?Glu?Gly?Phe?His?Tyr?Thr?Ala?Tyr?Asn?Val?Ile?Val

145 150 155 160

Asp?Arg?Val?Thr?Phe?Ser?Thr?Arg?Glu?Asp?Ala?Ser?Gln?Pro?Asn?Leu

165 170 175

Thr?Thr?Leu?Thr?Asn?Ser?Glu?Asn?Cys?Asp?Lys?Val?Ser?Ser?Leu?Ala

180 185 190

Leu?Leu?Ser?Ile?Val?Asp?Ser?Leu?Arg?Ile?Pro?Pro?Gln?Leu?Pro?Gln

195 200 205

Glu?Asp?Ile?Asp?Thr?Val?Ser?Met?Cys?Pro?Gln?Leu?Ala?Asp?Ala?Arg

210 215 220

Leu?Asn?Ser?Thr?Ser?Thr?Ser?Ser?Pro?Cys?Ser?Val?Ser?Ile?Ser?Pro

225 230 235 240

Glu?Val?Glu?Ser?Asn?Ile?Leu?Ala?Lys?Ile?Ala?Asp?Asn?Glu?Cys?Asn

245 250 255

Asn?Ala?Leu?His?Pro?Ala?Val?Ser?Cys?Thr?Thr?Glu?Glu?Thr?Lys?Glu

260 265 270

Gly?Ser?Ala?Ser?Ser?His?Asp?His?Gly?His?Ala?Val?Trp?Leu?Val?Ile

275 280 285

Thr?Leu?Ala?Phe?Ala?Phe?Leu?Phe

290 295

<210>158

<211>310

<212>PRT

＜213〉aspergillus niger

<400>158

Met?Gly?Gly?Arg?Asp?Val?Ala?Ile?Leu?Ser?Arg?His?Phe?Ala?Val?Thr

1 5 10 15

Ser?Ser?Gln?Ser?Val?Asn?Gly?Val?Val?Ser?Gly?Met?Phe?Gln?His?Thr

20 25 30

Val?Thr?Ser?Ser?Pro?Ser?Phe?Thr?Thr?Asn?Gln?Phe?Phe?Lys?Lys?Lys

35 40 45

Phe?Thr?Ala?Ala?Ile?Ala?Thr?Ala?Ile?Phe?Ala?Ser?Val?Ala?Val?Ala

50 55 60

Ala?Pro?Gln?Arg?Gly?Leu?Glu?Ala?Arg?Leu?Lys?Ala?Arg?Gly?Ser?Ser

65 70 75 80

Lys?Gly?Ser?Arg?Pro?Leu?Gln?Ala?Val?Ala?Arg?Pro?Ala?Ser?Thr?Lys

85 90 95

Asn?Gln?Thr?Asn?Val?Glu?Tyr?Ser?Ser?Asn?Trp?Ser?Gly?Ala?Val?Leu

100 105 110

Val?Glu?Pro?Pro?Ser?Ala?Ala?Ala?Thr?Tyr?Thr?Ala?Val?Thr?Gly?Thr

115 120 125

Phe?Thr?Val?Pro?Glu?Pro?Thr?Gly?Asn?Ser?Gly?Gly?Ser?Gln?Ala?Ala

130 135 140

Ser?Ala?Trp?Val?Gly?Ile?Asp?Gly?Asp?Thr?Tyr?Gly?Asn?Ala?Ile?Leu

145 150 155 160

Gln?Thr?Gly?Val?Asp?Phe?Thr?Val?Thr?Asp?Gly?Glu?Ala?Ser?Phe?Asp

165 170 175

Ala?Trp?Tyr?Glu?Trp?Tyr?Pro?Asp?Tyr?Ala?Tyr?Asp?Phe?Ser?Gly?Ile

180 185 190

Asp?Ile?Ser?Ala?Gly?Asp?Glu?Ile?Val?Ala?Ile?Val?Glu?Ser?Tyr?Thr

195 200 205

Ser?Thr?Thr?Gly?Ile?Ala?Ile?Ile?Glu?Asn?Lys?Ser?Thr?Gly?Gln?Lys

210 215 220

Val?Ser?Lys?Glu?Leu?Ser?Ser?Ser?Ser?Ser?Leu?Gly?Gly?Gln?Asn?Ala

225 230 235 240

Glu?Trp?Ile?Val?Glu?Asp?Phe?Glu?Glu?Asn?Gly?Ser?Leu?Val?Asn?Leu

245 250 255

Val?Asp?Phe?Gly?Thr?Val?Thr?Phe?Thr?Gly?Ala?Val?Ala?Lys?Ala?Ala

260 265 270

Gly?Gly?Glu?Ser?Val?Gly?Leu?Thr?Asp?Ala?Thr?Ile?Ile?Glu?Ile?Glu

275 280 285

Glu?Asn?Gly?Gln?Val?Val?Thr?Asp?Val?Thr?Ile?Asp?Ser?Asp?Ser?Glu

290 295 300

Val?Thr?Ile?Thr?Tyr?Glu

305 310

<210>159

<211>681

<212>PRT

＜213〉aspergillus niger

<400>159

Met?Arg?Cys?Ser?Leu?Ile?Ser?Leu?Leu?Gly?Leu?Ala?Ala?Ile?Pro?Ala

1 5 10 15

Leu?Gly?Gly?Cys?Pro?Phe?Ala?His?Thr?Ala?Asn?Met?Gly?Ile?Asp?Asn

20 25 30

Met?Val?Lys?Ala?His?Ala?His?Met?Ser?Arg?Pro?Leu?Ile?Ala?Ser?Lys

35 40 45

Ser?Ser?Pro?Ser?Thr?Val?Pro?Thr?Ser?Ser?Ser?Thr?Pro?Ser?Val?Gly

50 55 60

Gln?Lys?Gly?Val?Phe?Met?Met?Asn?Arg?Ile?Ala?Pro?Gly?Thr?Ser?Glu

65 70 75 80

Leu?TyrIle?Ala?Asn?Thr?Asp?Gly?Ser?Asn?Glu?Arg?Pro?Leu?Leu?Ser

85 90 95

Asn?Pro?Val?Tyr?Glu?Tyr?His?Ala?Ser?Phe?Ser?Pro?Asp?Val?Glu?Trp

100 105 110

Ile?Thr?Phe?Thr?Ser?Glu?Arg?Asn?Gly?Asp?Gly?Asn?Ser?Asp?Ile?Tyr

115 120 125

Arg?Val?Arg?Thr?Asn?Gly?Ser?Asp?Leu?Gln?Glu?Leu?Val?Ala?Thr?Pro

130 135 140

Ala?Val?Glu?Asp?Ser?Val?Val?Ile?Ser?Pro?Asn?Gly?Arg?Leu?Ala?Ala

145 150 155 160

Tyr?Val?Ser?Thr?Ala?Asn?Asn?Met?Lys?Ala?Asn?Ile?Trp?Ile?Leu?Asp

165 170 175

Leu?Gln?Thr?Gly?Ala?Gln?Trp?Asn?Leu?Thr?Asn?Thr?Pro?Thr?Thr?Ala

180 185 190

Ala?Asn?Ser?Ser?Leu?Met?Glu?Ser?Tyr?Leu?Arg?Pro?Ala?Trp?Ser?Pro

195 200 205

Asp?Gly?Glu?Trp?Ile?Ala?Phe?Ser?Ser?Asp?Arg?Asn?Thr?Gln?Trp?Asp

210 215 220

Gly?His?Gly?Val?Pro?Thr?Phe?Leu?Gly?Arg?Thr?Gly?Trp?Glu?Thr?Thr

225 230 235 240

Gln?Glu?Leu?Ser?Leu?Tyr?Ala?Ile?Arg?Pro?Asn?Gly?Ser?Asp?Phe?Arg

245 250 255

Gln?Ile?Ile?Ser?Lys?Pro?Tyr?Tyr?Ser?Leu?Gly?Ser?Pro?Lys?Trp?Ser

260 265 270

Ala?Asp?Gly?Lys?Arg?Ile?Val?Tyr?Tyr?Glu?Met?Thr?Arg?Glu?Asp?Thr

275 280 285

Tyr?Asn?Ala?His?Arg?Pro?Glu?Thr?Ile?Thr?Thr?Ala?Asn?Ser?Thr?Ile

290 295 300

Met?Ser?Val?Asp?Phe?Glu?Thr?Gly?Thr?Asp?Val?Arg?Val?Glu?Val?Ala

305 310 315 320

Gly?Ser?Gly?Val?Lys?Gln?Phe?Pro?Gln?Tyr?Leu?Asp?Lys?Asn?Gly?Thr

325 330 335

Ile?Ala?Tyr?Thr?Leu?Lys?Gly?Gly?Thr?Ser?Glu?Gly?Phe?Tyr?Thr?Thr

340 345 350

Ala?Gly?Leu?Tyr?Val?Asn?Thr?Thr?Ser?Ala?Thr?Leu?Arg?Ser?Pro?Ala

355 360 365

Trp?Ser?Pro?Asp?Gly?Lys?Gln?Val?Val?Tyr?Glu?Lys?Ser?Thr?Trp?Ser

370 375 380

Ile?Arg?Ser?Gly?Tyr?Lys?Gln?Leu?Tyr?Ser?Trp?Asp?Ser?Asp?Trp?Asp

385 390 395 400

Tyr?Arg?Phe?Thr?Asp?Val?Phe?Pro?Gln?Val?Ser?His?Gln?Glu?Arg?Val

405 410 415

Ala?Ile?Thr?Gln?Lys?Gln?Leu?Gly?Asn?Ser?Ser?Ile?Val?Thr?Leu?Asn

420 425 430

Thr?Thr?Gly?Gly?Asp?Leu?Gln?Leu?Val?Tyr?Asp?Pro?Ser?Thr?Ala?Asp

435 440 445

Phe?Val?Ser?Asp?Asp?Glu?Thr?Thr?Gly?Leu?Ser?Ala?Tyr?Gln?Pro?Ser

450 455 460

Trp?Ser?Pro?Cys?Gly?Glu?Trp?Leu?Val?Phe?Gly?Val?Gly?Phe?Trp?Phe

465 470 475 480

Glu?Thr?Arg?Glu?Ala?Ser?Gly?Gly?Trp?Ile?Val?Arg?Ala?Thr?Ala?Asn

485 490 495

Gly?Ser?Tyr?Ser?Glu?Val?Leu?Val?Asn?Ser?Ser?Tyr?Ser?Ile?Thr?Glu

500 505 510

Asp?Gly?Ala?Leu?Asn?Ser?Gly?Phe?Pro?Ser?Phe?Ser?Pro?Asp?Gly?Lys

515 520 525

Lys?Val?Val?Tyr?Arg?Val?Trp?Gly?Ala?Asp?Thr?Ala?Thr?Tyr?Gly?Asn

530 535 540

Ala?Ser?Glu?Ile?Gly?Leu?Arg?Val?Leu?Asp?Leu?Glu?Thr?Arg?Lys?Thr

545 550 555 560

Thr?Val?Leu?Thr?Thr?Glu?Trp?Asp?Asn?Leu?Pro?Gln?Phe?Ser?Pro?Asp

565 570 575

Gly?Glu?Leu?Ile?Leu?Phe?Thr?Arg?Lys?Thr?Ser?Thr?Tyr?Asn?Tyr?Asp

580 585 590

Val?Cys?Thr?Ile?Arg?Pro?Asp?Gly?Thr?Asp?Leu?Arg?Val?Leu?Thr?Ser

595 600 605

Ser?Gly?Ala?Asn?Asp?Ala?His?Ala?Val?Trp?Ser?Gln?Asp?Gly?Arg?Ile

610 615 620

Met?Trp?Ser?Thr?Gly?Met?Tyr?Gly?Phe?Arg?Phe?Glu?Cys?Ala?Leu?Tyr

625 630 635 640

Gly?Asp?Thr?Phe?Gln?Pro?Tyr?Gly?Gln?Val?Met?Ile?Met?Asp?Ala?Asp

645 650 655

Gly?Gly?Asn?Lys?Lys?Leu?Met?Thr?Asn?Ser?Met?Trp?Glu?Asp?Ser?Met

660 665 670

Pro?Leu?Phe?Leu?Pro?Arg?Glu?Val?Leu

675 680

<210>160

<211>624

<212>PRT

＜213〉aspergillus niger

<400>160

Met?Pro?Pro?Asp?Ala?Lys?Ser?Pro?Gly?Tyr?Gln?Pro?Gly?Met?Ala?Val

1 5 10 15

Leu?Pro?Ser?Arg?Pro?His?Pro?Ala?Lys?Gly?Lys?Ala?Ile?Arg?Phe?Leu

20 25 30

Leu?Ser?Leu?Ala?Leu?Val?Ala?Phe?Ala?Ile?Val?Gln?Leu?Cys?Gly?Asn

35 40 45

Phe?His?Lys?Asn?Arg?Ser?Val?Glu?Gln?Gln?Leu?Gln?Ser?Gln?Thr?Leu

50 55 60

Asp?Asp?Glu?Ser?Phe?Lys?Trp?Glu?Asp?Val?Thr?Pro?Thr?Lys?Gln?Leu

65 70 75 80

Val?Tyr?His?Pro?Cys?Phe?Gly?Asp?His?Glu?Cys?Ala?Arg?Leu?Ser?Leu

85 90 95

Pro?Met?Asn?Trp?Asn?Arg?Thr?Asp?Gly?Glu?Gly?Ser?Lys?Ile?Ala?Leu

100 105 110

Ala?Val?Ile?Lys?Leu?Pro?Ala?Lys?Val?Pro?Val?Thr?Asp?Ala?Arg?Tyr

115 120 125

Gly?Gly?Ala?Ile?Leu?Leu?Asn?Pro?Gly?Gly?Pro?Gly?Gly?Ser?Gly?Val

130 135 140

Ser?Met?Val?Phe?Arg?Tyr?Gly?Lys?Ala?Ile?Gln?Thr?Ile?Val?Asp?Ser

145 150 155 160

Pro?Glu?Ser?Pro?Ser?Ala?Asp?Ser?Ala?Ser?Gly?Lys?Tyr?Phe?Asp?Val

165 170 175

Val?Ser?Phe?Asp?Pro?Arg?Gly?Val?Asn?Asn?Thr?Thr?Pro?Asn?Phe?Ser

180 185 190

Cys?Phe?Pro?Asp?Pro?Ala?Thr?Arg?Lys?Ala?Trp?Leu?Leu?Gln?Ser?Glu

195 200 205

Ala?Glu?Gly?Leu?Leu?Gly?Ser?Ser?Glu?Gly?Val?Phe?Asp?Thr?Arg?Trp

210 215 220

Ala?Arg?Tyr?Glu?Ala?Phe?Glu?Arg?Leu?Leu?Ser?Thr?Ala?Pro?Asn?Thr

225 230 235 240

Phe?Pro?Val?Gly?Thr?Asn?Val?Asp?Ala?Glu?Arg?Ile?Arg?Leu?His?Asn

245 250 255

Arg?Trp?Lys?Lys?Gly?Glu?Glu?Lys?Leu?Leu?Tyr?Trp?Gly?Phe?Ser?Tyr

260 265 270

Gly?Thr?Ile?Leu?Gly?Ser?Thr?Phe?Ala?Ala?Met?Gln?Pro?His?Arg?Ile

275 280 285

Asn?Arg?Ala?Val?Ile?Asp?Gly?Val?Cys?Asn?Ala?Asp?Asp?Tyr?Tyr?Ala

290 295 300

Gly?Asn?Trp?Leu?Thr?Asn?Leu?Gln?Asp?Ser?Asp?Ala?Ala?Phe?Asn?Lys

305 310 315 320

Phe?Phe?Glu?Tyr?Cys?Tyr?Thr?Ala?Gly?Pro?Ser?Ala?Cys?Pro?Phe?Ala

325 330 335

Leu?Gly?Gly?Asp?Pro?Glu?Asp?Leu?Lys?Ser?Arg?Tyr?Glu?Gln?Ile?Leu

340 345 350

Thr?Asn?Leu?Thr?Ser?Ser?Pro?Ile?Ala?Val?Ser?Pro?Ser?Gly?Asn?Arg

355 360 365

Gly?Pro?Glu?Ile?Ile?Thr?Tyr?Ser?Asp?Val?Lys?Ser?Leu?Val?Val?Gln

370 375 380

Ala?Leu?Tyr?Val?Pro?Leu?Lys?Leu?Phe?Asp?Leu?Val?Ala?Arg?Leu?Leu

385 390 395 400

Ala?Glu?Leu?Glu?Gln?Gly?Asn?Gly?Ser?Ser?Phe?Ala?Asp?Leu?Lys?Tyr

405 4l0 415

Glu?Ala?Lys?Gln?Trp?Pro?Val?Pro?Pro?Pro?Cys?Asp?Ser?Ser?Ser?Thr

420 425 430

Gln?Tyr?Lys?Val?Pro?Gly?Glu?Ser?Asp?Gln?Glu?Ala?Gly?Arg?Asn?Ile

435 440 445

Leu?Cys?Thr?Asp?Gly?Pro?Gly?Leu?Asp?Gly?Thr?Ala?Lys?Glu?Asp?Phe

450 455 460

Arg?Ser?Tyr?Trp?Asn?Met?Leu?Arg?Gly?Gln?Ser?Lys?Ala?Val?Gly?Asp

465 470 475 480

Phe?Trp?Ala?Glu?Val?Arg?Met?Ser?Cys?Val?Lys?Leu?Glu?Thr?Arg?Pro

485 490 495

Glu?Trp?Arg?Tyr?Asp?Gly?Met?Arg?Ile?Gln?Gly?Pro?Phe?Ala?Gly?Asn

500 505 510

Thr?Ser?His?Pro?Leu?Leu?Phe?Ile?Gly?Asn?Thr?Tyr?Asp?Pro?Val?Thr

515 520 525

Pro?Leu?Arg?Asn?Ala?His?Thr?Met?Ala?Arg?Gly?Phe?Pro?Glu?Ser?Ile

530 535 540

Val?Leu?Glu?Gln?Asn?Ser?Val?Gly?His?Cys?Thr?Leu?Ser?Gly?Pro?Ser

545 550 555 560

Leu?Cys?Thr?Ala?Lys?Ala?Ile?Arg?Gln?Tyr?Phe?Gln?Thr?Gly?Glu?Leu

565 570 575

Pro?Asp?Pro?Gly?Thr?Val?Cys?Gln?Val?Glu?Glu?Leu?Pro?Phe?Arg?Leu

580 585 590

Ala?Gly?Tyr?Glu?Arg?Ser?Gln?Val?Met?Ser?Pro?Gly?Asp?Thr?Glu?Leu

595 600 605

Met?Ser?Ala?Leu?His?Ser?Leu?Ser?Glu?Phe?Arg?His?Leu?Leu?Gly?Ala

610 615 620

<210>161

<211>554

<212>PRT

＜213〉aspergillus niger

<400>161

Met?Leu?Ser?Ser?Leu?Leu?Leu?Gly?Gly?Leu?Leu?Gly?Leu?Ala?Thr?Ala

1 5 10 15

Gln?Phe?Pro?Pro?Glu?Pro?Glu?Gly?Ile?Thr?Val?Leu?Lys?Ser?Lys?Leu

20 25 30

His?Glu?Asn?Val?Thr?Ile?Ser?Phe?Lys?Glu?Pro?Gly?Ile?Cys?Glu?Thr

35 40 45

Thr?Pro?Gly?Val?Arg?Ser?Tyr?Ser?Gly?Tyr?Val?His?Leu?Pro?Pro?Ala

50 55 60

Ser?Thr?Ser?Phe?Phe?Trp?Phe?Phe?Glu?Ala?Arg?Lys?Asp?Pro?Ser?Asn

65 70 75 80

Ala?Pro?Leu?Ala?Ile?Trp?Leu?Asn?Gly?Gly?Pro?Gly?Gly?Ser?Ser?Leu

85 90 95

Met?Gly?Leu?Leu?Glu?Glu?Leu?Gly?Pro?Cys?Ser?Ile?Ala?Ser?Asp?Ser

100 105 110

Lys?Thr?Thr?Val?Leu?Asn?Pro?Trp?Ser?Trp?Asn?Asn?Glu?Val?Asn?Leu

115 120 125

Leu?Phe?Leu?Asp?Gln?Pro?Thr?Gln?Val?Gly?Phe?Ser?Tyr?Asp?Val?Pro

130 135 140

Thr?Asn?Gly?Thr?Leu?Thr?Ala?Asn?Gly?Thr?Ala?Phe?Ala?Ala?His?Ala

145 150 155 160

Leu?Trp?His?Phe?Ala?Gln?Thr?Trp?Phe?Phe?Glu?Phe?Pro?His?Tyr?Lys

165 170 175

Pro?Asn?Asp?Asp?Arg?Val?Ser?Leu?Trp?Ala?Glu?Ser?Tyr?Gly?Gly?His

180 185 190

Tyr?Gly?Pro?Gly?Ile?Phe?Arg?Phe?Phe?Gln?Gln?Gln?Asn?Asp?Lys?Ile

195 200 205

Ala?Glu?Gly?Thr?Ala?Glu?Asp?Gly?Ala?Gln?Tyr?Leu?His?Leu?Asp?Thr

210 215 220

Leu?Gly?Ile?Val?Asn?Gly?Leu?Met?Asp?Met?Val?Ile?Gln?Glu?Glu?Ala

225 230 235 240

Tyr?Ile?Thr?Trp?Pro?Tyr?Asn?Asn?Val?Arg?Leu?Ala?Pro?Ser?Ser?Phe

245 250 255

Asn?Ser?Arg?Gly?Phe?Arg?Asp?Gln?Ala?Leu?Ala?Cys?Glu?Ala?Ala?Leu

260 265 270

Lys?Glu?Arg?Asp?Ser?Gly?Leu?Pro?His?Ser?Gly?Lys?Asn?Ile?Ser?Glu

275 280 285

Ile?Cys?Gly?Gly?Leu?Ala?Leu?Glu?Trp?Gly?Asp?Gly?Pro?Ile?Thr?Tyr

290 295 300

Tyr?His?Thr?Phe?Asn?Arg?Gly?Trp?Tyr?Asp?Ile?Ala?His?Pro?Lys?Asn

305 310 315 320

Asp?Pro?Phe?Pro?Ala?Lys?His?Met?Leu?Gly?Tyr?Leu?Thr?Gln?Glu?Ser

325 330 335

Val?Leu?Ala?Ala?Leu?Gly?Val?Pro?Val?Asn?Phe?Thr?Ser?Ser?Ser?Ser

340 345 350

Ala?Val?Ala?Thr?Gln?Phe?Ile?Lys?Thr?Phe?Asp?Ile?Val?His?Gly?Gly

355 360 365

Phe?Leu?Asp?Ala?Ile?Gly?Tyr?Leu?Leu?Asp?Ser?Gly?Val?Lys?Val?His

370 375 380

Met?Met?Tyr?Gly?Asp?Arg?Asp?Tyr?Ala?Cys?Asn?Trp?Val?Gly?Gly?Glu

385 390 395 400

Lys?Ala?Ser?Leu?Ala?Val?Pro?Tyr?Ser?Arg?Ile?Thr?Glu?Phe?Ala?Asp

405 410 415

Thr?Gly?Tyr?Ser?Pro?Leu?Leu?Thr?Pro?Asp?Gly?Ile?Ser?Gly?Met?Thr

420 425 430

Arg?Gln?Leu?Gly?Asn?Tyr?Ser?Phe?Thr?Arg?Val?Phe?Gln?Ala?Gly?His

435 440 445

Glu?Val?Pro?Ser?Tyr?Gln?Pro?Val?Ala?Ala?Tyr?Glu?Ile?Phe?Met?Arg

450 455 460

Ala?Thr?Phe?Asn?Lys?Asp?Ile?Pro?Thr?Gly?Leu?Leu?Ala?Val?Asp?Asp

465 470 475 480

Glu?Phe?Gln?Ser?Val?Gly?Pro?Lys?Asp?Thr?Trp?His?Ile?Lys?Asn?Ile

485 490 495

Pro?Pro?Ile?Met?Pro?Lys?Pro?Gln?Cys?Tyr?Val?Leu?Ser?Pro?Gly?Thr

500 505 510

Cys?Thr?Pro?Glu?Val?Trp?Glu?Thr?Val?Leu?Asn?Gly?Ser?Ala?Thr?Val

515 520 525

Lys?Asp?Trp?Tyr?Val?Val?Asp?Asp?Ser?Ala?Gly?Val?Glu?Asp?His?Glu

530 535 540

Gly?Phe?Ser?Ile?Leu?Gly?Gly?Asp?Glu?Leu

545 550

<210>162

<211>578

<212>PRT

＜213〉aspergillus niger

<400>162

Met?Thr?Arg?Phe?Gln?Leu?Leu?Pro?Leu?Val?Ala?Gly?Leu?Leu?Ala?Pro

1 5 10 15

Ser?Ile?Ala?Ala?Leu?Ser?Ile?Pro?Ser?Pro?Gln?Gln?Ile?Leu?Asp?Ser

20 25 30

Leu?Thr?Phe?Gly?Glu?His?Thr?Asp?Gly?Phe?Cys?Pro?Leu?Ala?Pro?Lys

35 40 45

Val?Glu?Val?Pro?Asp?Asp?Gly?Phe?Phe?Pro?Ala?Leu?Lys?Phe?Val?Glu

50 55 60

Asp?Ala?Ser?Phe?Lys?Ser?Arg?Gln?Val?Asn?Arg?Leu?Ser?Arg?Ala?Val

65 70 75 80

Gln?Val?Pro?Thr?Ala?Ile?Asp?Asp?Tyr?Met?Lys?Asp?Pro?Tyr?Asp?Glu

85 90 95

Lys?Phe?Ala?Pro?Phe?Leu?Asp?Phe?Gln?Lys?Leu?Leu?Gln?Thr?Leu?Phe

100 105 110

Pro?Leu?Thr?His?Ser?Tyr?Ala?Arg?Val?Asp?His?Ile?Asn?Arg?Phe?Gly

115 120 125

Leu?Val?Phe?Thr?Leu?Asn?Gly?Thr?Asp?Asp?Ser?Leu?Lys?Pro?Leu?Leu

130 135 140

Phe?Thr?Ala?His?Gln?Asp?Val?Val?Pro?Ile?Asn?Asp?Pro?Ala?Asp?Trp

145 150 155 160

Thr?Tyr?Pro?Pro?Phe?Asp?Gly?His?Tyr?Asp?Gly?Glu?Trp?Leu?Trp?Gly

165 170 175

Arg?Gly?Ala?Ser?Asp?Cys?Lys?Asn?Val?Leu?Ile?Gly?Leu?Met?Ser?Val

180 185 190

Val?Glu?Asp?Leu?Leu?Ser?Gln?Lys?Trp?Glu?Pro?Thr?Arg?Thr?Val?Val

195 200 205

Leu?Ala?Phe?Gly?Phe?Asp?Glu?Glu?Ser?His?Gly?Phe?Leu?Gly?Ala?Gly

210 215 220

Ser?Ile?Ala?Lys?Phe?Leu?Glu?Lys?Lys?Tyr?Gly?Pro?Asp?Ser?Phe?Glu

225 230 235 240

Phe?Ile?Leu?Asp?Glu?Gly?Gly?Met?Gly?Leu?Glu?Val?Leu?Asp?Asp?Asn

245 250 255

Asn?Asn?Gly?Val?Val?Tyr?Ala?Leu?Pro?Gly?Val?Gly?Glu?Lys?Gly?Ser

260 265 270

Ile?Asp?Val?Val?Leu?Thr?Leu?Ala?Val?Pro?Gly?Gly?His?Ser?Ser?Val

275 280 285

Pro?Pro?Pro?His?Thr?Gly?Ile?Gly?Ile?Ile?Ala?Glu?Ile?Ile?Tyr?Glu

290 295 300

Leu?Glu?Arg?Gln?Asp?Leu?Phe?Val?Pro?Val?Leu?Asp?Thr?His?His?Pro

305 310 315 320

Thr?Arg?Lys?Met?Leu?Glu?Cys?Gln?Val?Arg?His?Ser?Pro?Ser?Gln?Val

325 330 335

Glu?Pro?Trp?Leu?Ala?Ser?Ala?Leu?Gln?Ser?Ser?Asp?Tyr?Ile?Ser?Leu

340 345 350

Ala?Glu?Lys?Leu?Ala?Ser?Ser?Arg?Gly?Asp?Lys?Phe?Arg?Phe?Ile?Leu

355 360 365

Gln?Thr?Ser?Gln?Ala?Ala?Asp?Ile?Ile?Asn?Gly?Gly?Val?Lys?Ser?Asn

370 375 380

Ala?Leu?Pro?Glu?Lys?Ile?Asn?Ala?Leu?Val?Asn?Tyr?Arg?Ile?Ala?Leu

385 390 395 400

His?Gln?Thr?Pro?Asp?Asp?Ile?Lys?Asn?Arg?Ala?Val?Glu?Ile?Ile?Ser

405 410 415

Pro?Ile?Val?Lys?Lys?Tyr?Asn?Leu?Ser?Leu?Thr?Ala?Phe?Pro?Glu?Ser

420 425 430

Asp?Thr?Val?Asp?Pro?Ser?Leu?Asn?Asn?His?Leu?Thr?Leu?Thr?Thr?Leu

435 440 445

Ser?Gly?Ala?Leu?Ser?Pro?Ala?Pro?Val?Ser?Pro?Thr?Asp?Ile?Asp?Thr

450 455 460

Asp?Ala?Val?Trp?Ala?Arg?Phe?Ser?Gly?Val?Thr?Arg?Ser?Val?Phe?Glu

465 470 475 480

Ser?Val?Pro?Ser?Leu?Glu?Gly?Arg?Lys?Val?Val?Val?Ser?Gly?Asp?Ile

485 490 495

Met?Thr?Gly?Asn?Thr?Asp?Thr?Arg?Phe?Tyr?Trp?Ala?Leu?Ser?Arg?Asn

500 505 510

Ile?Tyr?Arg?Trp?Ser?Pro?Ser?Arg?Ala?Gly?Lys?Ala?Leu?Asn?Ile?His

515 520 525

Thr?Val?Asp?Glu?Arg?Ile?Asp?Ile?Asp?Ile?His?Leu?Glu?Ala?Met?Met

530 535 540

Leu?Tyr?Tyr?Asp?Leu?Ile?Arg?Ser?Phe?Asp?Gly?Arg?Thr?Asp?Ser?Ser

545 550 555 560

Val?Ile?Ser?Ala?Ala?Ser?Ala?Ala?Ala?Asp?Asp?Glu?Leu?Ala?His?Asp

565 570 575

Val?Leu

<210>163

<211>456

<212>PRT

＜213〉aspergillus niger

<400>163

Met?Lys?Ser?Thr?Thr?Leu?Leu?Ser?Leu?Ala?Trp?Ala?Ala?Gln?Ser?Ala

1 5 10 15

Tyr?Ser?Leu?Ser?Ile?His?Glu?Arg?Asp?Glu?Pro?Ala?Thr?Leu?Gln?Phe

20 25 30

Asn?Phe?Glu?Arg?Arg?Gln?Ile?Ala?Asp?Arg?Ser?Arg?Arg?Lys?Arg?Ser

35 40 45

Thr?Ala?Ser?Ala?Asp?Leu?Val?Asn?Leu?Ala?Thr?Asn?Leu?Gly?Tyr?Thr

50 55 60

Met?Asn?Leu?Thr?Leu?Gly?Thr?Pro?Gly?Gln?Glu?Val?Ser?Val?Thr?Leu

65 70 75 80

Asp?Thr?Gly?Ser?Ser?Asp?Leu?Trp?Val?Asn?Gly?Ala?Asn?Ser?Ser?Val

85 90 95

Cys?Pro?Cys?Thr?Asp?Tyr?Gly?Ser?Tyr?Asn?Ser?Ser?Ala?Ser?Ser?Thr

100 105 110

Tyr?Thr?Phe?Val?Asn?Asp?Glu?Phe?Tyr?Ile?Gln?Tyr?Val?Asp?Gly?Ser

115 120 125

Glu?Ala?Thr?Gly?Asp?Tyr?Val?Asn?Asp?Thr?Leu?Lys?Phe?Ser?Asn?Val

130 135 140

Thr?Leu?Thr?Asn?Phe?Gln?Phe?Ala?Val?Ala?Tyr?Asp?Gly?Asp?Ser?Glu

145 150 155 160

Glu?Gly?Val?Leu?Gly?Ile?Gly?Tyr?Ala?Ser?Asn?Glu?Ala?Ser?Gln?Ala

165 170 175

Thr?Val?Gly?Gly?Gly?Glu?Tyr?Thr?Asn?Phe?Pro?Glu?Ala?Leu?Val?Asp

180 185 190

Gln?Gly?Ala?Ile?Asn?Trp?Pro?Ala?Tyr?Ser?Leu?Trp?Leu?Asp?Asp?Leu

195 200 205

Asp?Glu?Gly?Lys?Gly?Thr?Ile?Leu?Phe?Gly?Gly?Val?Asn?Thr?Ala?Lys

210 215 220

Tyr?Tyr?Gly?Ser?Leu?Gln?Thr?Leu?Pro?Ile?Val?Ser?Ile?Glu?Asp?Met

225 230 235 240

Tyr?Val?Glu?Phe?Ala?Val?Asn?Leu?Thr?Ala?Val?His?Leu?Glu?Lys?Asn

245 250 255

Gly?Asn?Ser?Val?Ser?Val?Asn?Asn?Ser?Ala?Thr?Gln?Phe?Pro?Ile?Pro

260 265 270

Ala?Val?Leu?Asp?Ser?Gly?Thr?Ala?Leu?Thr?Tyr?Ile?Pro?Thr?Ser?Ala

275 280 285

Ala?Ala?Ser?Ile?Tyr?Glu?Ala?Val?Gly?Ala?Gln?Tyr?Leu?Ser?Glu?Tyr

290 295 300

Gly?Tyr?Gly?Val?Ile?Glu?Cys?Asp?Val?Lys?Asp?Glu?Asp?Phe?Thr?Phe

305 310 315 320

Leu?Phe?Asp?Phe?Gly?Ser?Phe?Asn?Met?Ser?Val?Asp?Ile?Ser?Glu?Met

325 330 335

Ile?Leu?Glu?Ala?Ser?Ser?Asp?Met?Thr?Asp?Met?Asn?Val?Cys?Thr?Phe

340 345 350

Gly?Leu?Ala?Val?Ile?Glu?Asn?Glu?Ala?Leu?Leu?Gly?Asp?Thr?Phe?Leu

355 360 365

Arg?Ser?Ala?Tyr?Val?Val?Tyr?Asp?Leu?Gly?Asn?Asn?Glu?Ile?Ser?Leu

370 375 380

Ala?Lys?Ala?Asn?Phe?Asn?Pro?Gly?Glu?Asp?His?Val?Leu?Glu?Ile?Gly

385 390 395 400

Thr?Gly?Ser?Asp?Ala?Val?Pro?Lys?Ala?Thr?Gly?Ala?Thr?Ala?Thr?Gly

405 410 415

Ala?Ala?Ala?Thr?Ser?Thr?Ala?Ser?Ser?Asp?Lys?Ser?Asp?Lys?Glu?Ser

420 425 430

Ser?Ala?Thr?Val?Pro?Arg?Ser?Gln?Ile?Val?Ser?Leu?Val?Ala?Gly?Val

435 440 445

Leu?Val?Gly?Val?Phe?Leu?Val?Leu

450 455

<210>164

<211>664

<212>PRT

＜213〉aspergillus niger

<400>164

Met?Leu?Val?Arg?Gln?Leu?Ala?Leu?Ala?Leu?Ala?Ile?Ala?Ala?Leu?Ser

1 5 10 15

Asp?Ala?Ile?Pro?Thr?Ser?Ile?Lys?His?Val?Leu?His?Glu?Lys?Arg?His

20 25 30

Lys?Pro?Ala?Ser?Asp?Trp?Val?Lys?Gly?Ala?Arg?Val?Glu?Ser?Asp?Ala

35 40 45

Val?Leu?Pro?Met?Arg?Ile?Gly?Leu?Ala?Gln?Asn?Asn?Leu?Asp?Lys?Gly

50 55 60

Tyr?Asp?Phe?Leu?Met?Glu?Val?Ser?Asp?Pro?Lys?Ser?Ser?Lys?Tyr?Gly

65 70 75 80

Gln?Tyr?Trp?Ser?Ala?Asp?Glu?Val?His?Asp?Ile?Phe?Ser?Pro?Ser?Glu

85 90 95

Glu?Ala?Val?Glu?Ala?Val?Arg?Glu?Trp?Leu?Val?Ala?Ser?Gly?Ile?His

100 105 110

Pro?Ser?Arg?Val?Val?His?Ser?Asp?Asn?Lys?Gly?Trp?Leu?Ala?Phe?Asp

115 120 125

Ala?Tyr?Ala?His?Glu?Ala?Glu?Arg?Leu?Phe?Met?Thr?Glu?Phe?His?Glu

130 135 140

His?Glu?Ser?Asp?Arg?Ser?Ala?Lys?Ile?Arg?Val?Gly?Cys?Asp?Gln?Tyr

145 150 155 160

His?Val?Pro?Glu?His?Ile?Gln?Lys?His?Ile?Asp?Tyr?Ile?Thr?Pro?Gly

165 170 175

Val?Lys?Leu?Thr?Gln?Val?Val?Lys?Arg?Thr?Asn?Lys?Val?Lys?Arg?Ala

180 185 190

Ser?Gln?Leu?Ala?His?Ser?Ser?Lys?Ala?Lys?Ser?Ala?Ala?Gln?Gly?Pro

195 200 205

Gln?Pro?Leu?Pro?Asn?Lys?Ala?Lys?Phe?Leu?Pro?Glu?Asp?Leu?Arg?Gly

210 215 220

Cys?Gly?Tyr?Asn?Ile?Thr?Pro?Ser?Cys?Ile?Lys?Ala?Leu?Tyr?Gln?Ile

225 230 235 240

Pro?Asp?Ala?Lys?Thr?Ala?Thr?Pro?Asn?Asn?Ser?Leu?Gly?Leu?Tyr?Glu

245 250 255

Gln?Gly?Asp?Tyr?Phe?Ala?Lys?Ser?Asp?Leu?Asp?Leu?Phe?Tyr?Lys?Glu

260 265 270

Tyr?Ala?Pro?Trp?Val?Pro?Gln?Gly?Thr?Tyr?Pro?Ile?Pro?Ala?Leu?Ile

275 280 285

Asp?Gly?Ala?Asn?Tyr?Ser?Val?Pro?Ser?Tyr?Ser?Ser?Leu?Asn?Thr?Gly

290 295 300

Glu?Ser?Asp?Ile?Asp?Ile?Asp?Met?Ala?Tyr?Ser?Leu?Leu?Tyr?Pro?Gln

305 310 315 320

Gln?Val?Thr?Leu?Tyr?Gln?Val?Asp?Asp?Gln?Leu?Tyr?Glu?Pro?Val?Glu

325 330 335

Val?Asp?Thr?Thr?Asn?Leu?Phe?Asn?Thr?Phe?Leu?Asp?Ala?Leu?Asp?Gly

340 345 350

Ser?Tyr?Cys?Thr?Tyr?Ser?Ala?Tyr?Gly?Glu?Thr?Gly?Asp?Asp?Pro?Ser

355 360 365

Ile?Asp?Pro?Val?Tyr?Pro?Asp?Thr?Arg?Pro?Gly?Gly?Tyr?Lys?Gly?Lys

370 375 380

Leu?Gln?Cys?Gly?Val?Tyr?Lys?Pro?Thr?Asn?Val?Ile?Ser?Ala?Ser?Tyr

385 390 395 400

Gly?Gln?Ser?Glu?Ala?Asp?Leu?Pro?Val?Ser?Tyr?Thr?Lys?Arg?Gln?Cys

405 410 415

Asn?Glu?Phe?Met?Lys?Leu?Gly?Leu?Gln?Gly?His?Ser?Ile?Leu?Phe?Ala

420 425 430

Ser?Gly?Asp?Tyr?Gly?Val?Ala?Ser?Phe?Ala?Gly?Asp?Gly?Asp?Glu?Asn

435 440 445

Gly?Cys?Leu?Gly?Pro?Glu?Gly?Lys?Ile?Phe?Asn?Pro?Gln?Tyr?Pro?Ser

450 455 460

Asn?Cys?Pro?Tyr?Val?Thr?Ser?Val?Gly?Gly?Thr?Met?Leu?Tyr?Gly?Tyr

465 470 475 480

Gln?Thr?Val?Asn?Asp?Ser?Glu?Ser?Val?Met?His?Val?Asn?Leu?Gly?Gly

485 490 495

Thr?Ala?Ser?Asn?Phe?Ser?Thr?Ser?Gly?Gly?Phe?Ser?Asn?Tyr?Phe?Pro

500 505 510

Gln?Pro?Ala?Tyr?Gln?Phe?Ala?Ala?Val?Glu?Gln?Tyr?Phe?Gln?Ser?Ala

515 520 525

Asn?Leu?Ser?Tyr?Pro?Tyr?Tyr?Ser?Glu?Phe?Glu?Val?Asp?Val?Asn?Thr

530 535 540

Thr?Lys?Gly?Leu?Tyr?Asn?Arg?Leu?Gly?Arg?Ala?Tyr?Pro?Asp?Val?Ser

545 550 555 560

Ala?Asn?Gly?Ala?His?Phe?Arg?Ala?Tyr?Met?Asp?Gly?Tyr?Asp?Tyr?His

565 570 575

Trp?Tyr?Gly?Ser?Ser?Leu?Ala?Ser?Pro?Leu?Phe?Ala?Ser?Val?Leu?Thr

580 585 590

Leu?Leu?Asn?Glu?Glu?Arg?Phe?Ala?Ile?Gly?Lys?Gly?Pro?Val?Gly?Phe

595 600 605

Val?Asn?Pro?Val?Leu?Tyr?Ala?Tyr?Pro?Gln?Val?Leu?Asn?Asp?Ile?Thr

610 615 620

Asn?Gly?Thr?Asn?Ala?Gly?Cys?Gly?Thr?Tyr?Gly?Phe?Ser?Ala?Ile?Glu

625 630 635 640

Gly?Trp?Asp?Pro?Ala?Ser?Gly?Leu?Gly?Thr?Pro?Asn?Tyr?Pro?Leu?Met

645 650 655

Lys?Glu?Leu?Phe?Leu?Ser?Leu?Pro

660

<210>165

<211>520

<212>PRT

＜213〉aspergillus niger

<400>165

Met?Arg?Val?Thr?Thr?Ala?Ile?Ala?Ser?Leu?Leu?Leu?Val?Gly?Ser?Ala

1 5 10 15

Thr?Ser?Leu?Gln?Asn?Pro?His?Arg?Arg?Ala?Val?Pro?Pro?Pro?Leu?Ser

20 25 30

His?Arg?Ser?Val?Ala?Ser?Arg?Ser?Val?Pro?Val?Glu?Arg?Arg?Thr?Thr

35 40 45

Asp?Phe?Glu?Tyr?Leu?Thr?Asn?Lys?Thr?Ala?Arg?Phe?Leu?Val?Asn?Gly

50 55 60

Thr?Ser?Ile?Pro?Glu?Val?Asp?Phe?Asp?Val?Gly?Glu?Ser?Tyr?Ala?Gly

65 70 75 80

Leu?Leu?Pro?Asn?Thr?Pro?Thr?Gly?Asn?Ser?Ser?Leu?Phe?Phe?Trp?Phe

85 90 95

Phe?Pro?Ser?Gln?Asn?Pro?Glu?Ala?Ser?Asp?Glu?Ile?Thr?Ile?Trp?Leu

100 105 110

Asn?Gly?Gly?Pro?Gly?Cys?Ser?Ser?Leu?Asp?Gly?Leu?Leu?Gln?Glu?Asn

115 120 125

Gly?Pro?Phe?Leu?Trp?Gln?Pro?Gly?Thr?Tyr?Lys?Pro?Val?Pro?Asn?Pro

130 135 140

Tyr?Ser?Trp?Thr?Asn?Leu?Thr?Asn?Val?Val?Tyr?Ile?Asp?Gln?Pro?Ala

145 150 155 160

Gly?Thr?Gly?Phe?Ser?Pro?Gly?Pro?Ser?Thr?Val?Asn?Asn?Glu?Glu?Asp

165 170 175

Val?Ala?Ala?Gln?Phe?Asn?Ser?Trp?Phe?Lys?His?Phe?Val?Asp?Thr?Phe

180 185 190

Asp?Leu?His?Gly?Arg?Lys?Val?Tyr?Ile?Thr?Gly?Glu?Ser?Tyr?Ala?Gly

195 200 205

Met?Tyr?Val?Pro?Tyr?Ile?Ala?Asp?Ala?Met?Leu?Asn?Glu?Glu?Asp?Thr

210 215 220

Thr?Tyr?Phe?Asn?Leu?Lys?Gly?Ile?Gln?Ile?Asn?Asp?Pro?Ser?Ile?Asn

225 230 235 240

Ser?Asp?Ser?Val?Met?Met?Tyr?Ser?Pro?Ala?Val?Arg?His?Leu?Asn?His

245 250 255

Tyr?Asn?Asn?Ile?Phe?Gln?Leu?Asn?Ser?Thr?Phe?Leu?Ser?Tyr?Ile?Asn

260 265 270

Ala?Lys?Ala?Asp?Lys?Cys?Gly?Tyr?Asn?Ala?Phe?Leu?Asp?Lys?Ala?Ile

275 280 285

Thr?Tyr?Pro?Pro?Pro?Ser?Pro?Phe?Pro?Thr?Ala?Pro?Glu?Ile?Thr?Glu

290 295 300

Asp?Cys?Gln?Val?Trp?Asp?Glu?Val?Val?Met?Ala?Ala?Tyr?Asp?Ile?Asn

305 310 315 320

Pro?Cys?Phe?Asn?Tyr?Tyr?His?Leu?Ile?Asp?Phe?Cys?Pro?Tyr?Leu?Trp

325 330 335

Asp?Val?Leu?Gly?Phe?Pro?Ser?Leu?Ala?Ser?Gly?Pro?Asn?Asn?Tyr?Phe

340 345 350

Asn?Arg?Ser?Asp?Val?Gln?Lys?Ile?Leu?His?Val?Pro?Pro?Thr?Asp?Tyr

355 360 365

Ser?Val?Cys?Ser?Glu?Thr?Val?Ile?Phe?Ala?Asn?Gly?Asp?Gly?Ser?Asp

370 375 380

Pro?Ser?Ser?Trp?Gly?Pro?Leu?Pro?Ser?Val?Ile?Glu?Arg?Thr?Asn?Asn

385 390 395 400

Thr?Ile?Ile?Gly?His?Gly?Trp?Leu?Asp?Tyr?Leu?Leu?Phe?Leu?Asn?Gly

405 410 415

Ser?Leu?Ala?Thr?Ile?Gln?Asn?Met?Thr?Trp?Asn?Gly?Lys?Gln?Gly?Phe

420 425 430

Gln?Arg?Pro?Pro?Val?Glu?Pro?Leu?Phe?Val?Pro?Tyr?His?Tyr?Gly?Leu

435 440 445

Ala?Glu?Leu?Tyr?Trp?Gly?Asp?Glu?Pro?Asp?Pro?Tyr?Asn?Leu?Asp?Ala

450 455 460

Gly?Ala?Gly?Tyr?Leu?Gly?Thr?Ala?His?Thr?Glu?Arg?Gly?Leu?Thr?Phe

465 470 475 480

Ser?Ser?Val?Tyr?Leu?Ser?Gly?His?Glu?Ile?Pro?Gln?Tyr?Val?Pro?Gly

485 490 495

Ala?Ala?Tyr?Arg?Gln?Leu?Glu?Phe?Leu?Leu?Gly?Arg?Ile?Ser?Ser?Leu

500 505 510

Ser?Ala?Lys?Gly?Asn?Tyr?Thr?Ser

515 520

<210>166

<211>551

<212>PRT

＜213〉aspergillus niger

<400>166

Met?Arg?Gly?Ser?Arg?Leu?Val?Leu?Leu?Leu?Pro?Leu?Ala?Ala?Leu?Ser

1 5 10 15

Cys?Ala?Met?Pro?Glu?Asn?Glu?Trp?Ser?Ser?Thr?Ile?Arg?Arg?Gln?Leu

20 25 30

Pro?Lys?Ala?Ser?Thr?Gly?Val?Lys?Ser?Ile?Lys?Thr?Pro?Asn?Asn?Val

35 40 45

Thr?Ile?Arg?Tyr?Lys?Glu?Pro?Gly?Thr?Glu?Gly?Ile?Cys?Glu?Thr?Thr

50 55 60

Pro?Gly?Val?Lys?Ser?Tyr?Ser?Gly?Tyr?Val?Asp?Leu?Ser?Pro?Glu?Ser

65 70 75 80

His?Thr?Phe?Phe?Trp?Phe?Phe?Glu?Ser?Arg?Arg?Asp?Pro?Glu?Asn?Asp

85 90 95

Pro?Val?Thr?Leu?Trp?Leu?Asn?Gly?Gly?Pro?Gly?Ser?Asp?Ser?Leu?Ile

100 105 110

Gly?Leu?Phe?Glu?Glu?Leu?Gly?Pro?Cys?His?Ile?Thr?Pro?Glu?Tyr?Glu

115 120 125

Ser?Ile?Ile?Asn?Gln?Tyr?Ser?Trp?Asn?Glu?Val?Thr?Asn?Leu?Leu?Phe

130 135 140

Leu?Ser?Gln?Pro?Leu?Gly?Val?Gly?Phe?Ser?Tyr?Ser?Glu?Thr?Glu?Ala

145 150 155 160

Gly?Ser?Leu?Asn?Pro?Phe?Thr?Gly?Ala?Val?Glu?Asn?Ala?Ser?Phe?Ala

165 170 175

Gly?Val?Gln?Gly?Arg?Tyr?Pro?Val?Ile?Asp?Ala?Thr?Ile?Ile?Asp?Thr

180 185 190

Thr?Asp?Ile?Ala?Ala?Arg?Ala?Thr?Trp?Glu?Val?Leu?Gln?Gly?Phe?Leu

195 200 205

Ser?Gly?Leu?Ser?Gln?Leu?Asp?Ser?Glu?Val?Lys?Ser?Lys?Glu?Phe?Asn

210 215 220

Leu?Trp?Thr?Glu?Ser?Tyr?Gly?Gly?His?Tyr?Gly?Pro?Ala?Phe?Phe?Asn

225 230 235 240

His?Phe?Tyr?Glu?Gln?Asn?Ser?Lys?Ile?Ala?Ser?Gly?Glu?Val?Asn?Gly

245 250 255

Val?Gln?Leu?Asn?Phe?Asn?Ser?Leu?Gly?Ile?Ile?Asn?Gly?Ile?Ile?Asp

260 265 270

Ala?Ala?Ile?Gln?Ala?Asp?Tyr?Tyr?Ala?Asp?Phe?Ala?Val?Asn?Asn?Thr

275 280 285

Tyr?Gly?Ile?Lys?Ala?Val?Asn?Asp?Thr?Val?Tyr?Asn?Tyr?Met?Lys?Phe

290 295 300

Ala?Asn?Thr?Met?Pro?Asn?Gly?Cys?Gln?Asp?Gln?Val?Ala?Ser?Cys?Lys

305 310 315 320

Leu?Thr?Asn?Arg?Thr?Ser?Leu?Ser?Asp?Tyr?Ala?Ile?Cys?Thr?Glu?Ala

325 330 335

Ala?Asn?Met?Cys?Arg?Asp?Asn?Val?Glu?Gly?Pro?Tyr?Tyr?Gln?Phe?Gly

340 345 350

Gly?Arg?Gly?Val?Tyr?Asp?Ile?Arg?His?Pro?Tyr?Asn?Asp?Pro?Thr?Pro

355 360 365

Pro?Ser?Tyr?Phe?Val?Asp?Tyr?Leu?Lys?Lys?Asp?Ser?Val?Met?Asp?Ala

370 375 380

Ile?Gly?Val?Asp?Ile?Asn?Tyr?Thr?Glu?Ser?Ser?Gly?Glu?Val?Tyr?Tyr

385 390 395 400

Ala?Phe?Gln?Gln?Thr?Gly?Asp?Phe?Val?Trp?Pro?Asn?Phe?Ile?Glu?Asp

405 410 415

Leu?Glu?Glu?Ile?Leu?Gln?Leu?Pro?Val?Arg?Val?Ser?Leu?Ile?Tyr?Gly

420 425 430

Asp?Ala?Asp?Tyr?Ile?Cys?Asn?Trp?Phe?Gly?Gly?Gln?Ala?Ile?Ser?Leu

435 440 445

Ala?Val?Asn?Tyr?Pro?His?Ala?Ala?Gln?Phe?Arg?Ala?Ala?Gly?Tyr?Thr

450 455 460

Pro?Met?Thr?Val?Asp?Gly?Val?Glu?Tyr?Gly?Glu?Thr?Arg?Glu?Tyr?Gly

465 470 475 480

Asn?Phe?Ser?Phe?Thr?Arg?Val?Tyr?Gln?Ala?Gly?His?Glu?Val?Pro?Tyr

485 490 495

Tyr?Gln?Pro?Ile?Ala?Ala?Leu?Gln?Leu?Phe?Asn?Arg?Thr?Leu?Phe?Gly

500 505 510

Trp?Asp?Ile?Ala?Ala?Gly?Thr?Thr?Gln?Ile?Trp?Pro?Glu?Tyr?Ser?Thr

515 520 525

Asn?Gly?Thr?Ser?Gln?Ala?Thr?His?Thr?Glu?Ser?Phe?Val?Pro?Leu?Ser

530 535 540

Thr?Ala?Ser?Ser?Thr?Val?Asn

545 550

<210>167

<211>623

<212>PRT

＜213〉aspergillus niger

<400>167

Met?Pro?Phe?Pro?Phe?Ser?Ser?Ala?Leu?Leu?Gly?Tyr?Ile?Leu?Thr?Thr

1 5 10 15

Ser?Thr?Thr?Leu?Thr?Ser?Leu?Val?Ala?Gly?Gln?Tyr?Tyr?Pro?Pro?Thr

20 25 30

Pro?Glu?Asp?Leu?Thr?Val?Ile?His?Ser?Glu?Ile?Phe?Pro?Gly?Ala?Arg

35 40 45

Ile?Ser?Tyr?Lys?Gln?Pro?Leu?Gly?Ile?Cys?Thr?Thr?Thr?Pro?Ser?Thr

50 55 60

Pro?Ser?Tyr?Ser?Gly?Tyr?Ile?His?Leu?Pro?Pro?His?Thr?Leu?Thr?Asn

65 70 75 80

Leu?Ser?Ile?Pro?Gly?Ile?Ser?Ile?Ser?Gln?Pro?Tyr?Pro?Ile?Asn?Thr

85 90 95

Phe?Phe?Trp?Tyr?Phe?Pro?Ser?Arg?His?His?His?Asn?Asn?Asp?Thr?Ser

100 105 110

Pro?Leu?Thr?Ile?Trp?Met?Asn?Gly?Gly?Pro?Gly?Gly?Ser?Ser?Met?Ile

115 120 125

Gly?Leu?Phe?Gln?Glu?Asn?Gly?Pro?Cys?Thr?Val?Asn?Thr?Asp?Ser?Asn

130 135 140

Ser?Thr?Ala?Tyr?Asn?Pro?Trp?Ser?Trp?Asn?Glu?Tyr?Val?Asp?Met?Leu

145 150 155 160

Tyr?Ile?Glu?Gln?Pro?Val?Gln?Thr?Gly?Phe?Ser?Tyr?Asp?Val?Leu?Arg

165 170 175

Asn?Gly?Thr?Leu?Asp?Leu?Asn?Glu?Thr?Phe?Leu?Val?Gly?Thr?Leu?Pro

180 185 190

Ser?Gln?Asp?Val?His?Gly?Thr?Val?Asn?Gly?Thr?Val?Asn?Gly?Gly?Arg

195 200 205

Ala?Leu?Trp?Val?Ala?Leu?Gln?Val?Trp?Leu?Gly?Glu?Phe?Ser?Glu?Tyr

210 215 220

Val?Ser?Ser?Val?Asp?Gly?Asn?Gly?Gly?Gly?Asp?Asp?Arg?Val?Ser?Ile

225 230 235 240

Trp?Thr?Glu?Ser?Tyr?Gly?Gly?Arg?Tyr?Gly?Pro?Ala?Tyr?Thr?Ala?Leu

245 250 255

Phe?Gln?Glu?Met?Asn?Glu?Arg?Ile?Glu?Ser?Gly?Glu?Val?Ser?Thr?Gly

260 265 270

Lys?Lys?Ile?His?Leu?Asp?Thr?Leu?Gly?Ile?Ile?Asn?Gly?Cys?Val?Asp

275 280 285

Leu?Leu?Val?Gln?Val?Pro?Ser?Phe?Pro?Glu?Gln?Ala?Tyr?Asn?Asn?Thr

290 295 300

Tyr?Gly?Ile?Glu?Gly?Ile?Asn?Arg?Thr?Leu?Tyr?Asp?Arg?Ala?Met?Asp

305 310 315 320

Ser?Trp?Ser?Lys?Pro?Gly?Gly?Cys?Arg?Asp?Met?Ile?Ile?Glu?Cys?Arg

325 330 335

Asp?Ala?Gly?Glu?Leu?Gly?Asp?Pro?Leu?Ile?Ile?Cys?Glu?Glu?Ala?Ser

340 345 350

Asp?Tyr?Cys?Ser?Arg?Glu?Ile?Lys?Ser?Leu?Tyr?Thr?Asn?Thr?Ser?Gly

355 360 365

Arg?Gly?Tyr?Tyr?Asp?Ile?Ala?His?Phe?Thr?Pro?Asp?Ala?Ala?Leu?Val

370 375 380

Pro?Tyr?Phe?Val?Gly?Phe?Leu?Asn?Arg?Pro?Trp?Val?Gln?Lys?Ala?Leu

385 390 395 400

Gly?Val?Pro?Val?Asn?Tyr?Thr?Met?Ser?Ser?Glu?Ala?Val?Gly?Asn?Ser

405 410 415

Phe?Ala?Ser?Thr?Gly?Asp?Tyr?Pro?Arg?Asn?Asp?Pro?Arg?Gly?Met?Ile

420 425 430

Gly?Asp?Ile?Gly?Tyr?Leu?Leu?Asp?Ser?Gly?Val?Lys?Val?Ala?Met?Val

435 440 445

Tyr?Gly?Asp?Arg?Asp?Tyr?Ala?Cys?Pro?Trp?Arg?Gly?Gly?Glu?Asp?Val

450 455 460

Ser?Leu?Leu?Val?Glu?Tyr?Glu?Asp?Ala?Glu?Lys?Phe?Arg?Ala?Ala?Gly

465 470 475 480

Tyr?Ala?Glu?Val?Gln?Thr?Lys?Ser?Ser?Tyr?Val?Gly?Gly?Leu?Val?Arg

485 490 495

Gln?Tyr?Gly?Asn?Phe?Ser?Phe?Thr?Arg?Val?Phe?Gln?Ala?Gly?His?Glu

500 505 510

Val?Pro?Phe?Tyr?Gln?Pro?Glu?Thr?Ala?Tyr?Glu?Ile?Phe?Asn?Arg?Ala

515 520 525

Gln?Phe?Asn?Trp?Asp?Ile?Ala?Thr?Gly?Gly?Ile?Ser?Leu?Glu?Gln?Asn

530 535 540

Gln?Ser?Tyr?Gly?Thr?Glu?Gly?Pro?Ser?Ser?Thr?Trp?His?Ile?Lys?Asn

545 550 555 560

Glu?Val?Pro?Glu?Ser?Pro?Glu?Pro?Thr?Cys?Tyr?Leu?Leu?Ala?Met?Asp

565 570 575

Ser?Thr?Cys?Thr?Asp?Glu?Gln?Arg?Glu?Arg?Val?Leu?Ser?Gly?Asp?Ala

580 585 590

Val?Val?Arg?Asp?Trp?Val?Val?Val?Asp?Asp?Ile?Glu?Ala?Glu?Ser?Ser

595 600 605

Phe?Ser?Gly?Val?Gly?Asp?Gln?Leu?Ala?Gln?Val?Pro?Leu?Gly?His

610 615 620

<210>168

<211>439

<212>PRT

＜213〉aspergillus niger

<400>168

Met?Arg?Thr?Ser?Thr?Leu?Leu?Leu?Leu?Trp?Ser?Thr?Ala?Gly?Ala?Ala

1 5 10 15

Leu?Ala?Ser?Pro?Tyr?Pro?Leu?Pro?Asp?Ser?Gln?Val?Val?Phe?Ala?Ala

20 25 30

Asp?His?Glu?Val?Pro?Asn?Thr?Gln?Gly?Lys?His?Val?Val?Asp?Glu?Ala

35 40 45

Ile?Leu?Ser?Ala?Leu?Asn?Ala?His?Ser?Asp?Pro?Val?Ala?Ala?Met?Val

50 55 60

Ser?Leu?Arg?Pro?Glu?Thr?Ala?Ala?Phe?Leu?Ala?Glu?Pro?Arg?Leu?Leu

65 70 75 80

His?Ile?Arg?Gly?Glu?Glu?Lys?Ala?Glu?Trp?Met?Thr?Glu?Gly?Asp?Lys

85 90 95

Leu?Arg?Leu?Arg?Gln?Arg?Gly?Lys?Lys?Phe?Met?Asp?Ile?Thr?Glu?His

100 105 110

Gln?Asp?Phe?Tyr?Ala?Glu?Gln?Ala?Met?Ala?Ser?Phe?Ala?Gly?Asp?Pro

115 120 125

Asn?Leu?Pro?Lys?Leu?Ser?His?Lys?Gly?Leu?Val?Lys?Pro?Leu?Phe?Ser

130 135 140

Gln?Ile?Glu?Thr?Glu?Arg?Met?His?Asp?Ile?Leu?Gln?His?Met?Thr?Ser

145 150 155 160

Tyr?Tyr?Asn?Arg?Tyr?Tyr?Gly?Asp?Tyr?His?Gly?Glu?Met?Ser?Ser?Glu

165 170 175

Trp?Leu?His?Asp?Tyr?Ile?Ala?Ala?Ile?Ile?Ser?Lys?Ser?Pro?Phe?Arg

180 185 190

Thr?His?Ile?Ser?Leu?Glu?Tyr?Phe?Thr?His?Pro?Phe?Arg?Gln?Ser?Ser

195 200 205

Ile?Ile?Ala?Arg?Phe?Glu?Pro?Lys?Val?Arg?Ser?Phe?Ser?Gln?Pro?Leu

210 215 220

Thr?Ile?Ile?Gly?Ala?His?Gln?Asp?Ser?Ala?Asn?Tyr?Leu?Phe?Pro?Leu

225 230 235 240

Leu?Pro?Ala?Pro?Gly?Ala?Asp?Asp?Asp?Cys?Ser?Gly?Thr?Val?Ser?Ile

245 250 255

Leu?Glu?Ala?Phe?Arg?Val?Leu?Ala?Glu?Asn?Gly?Tyr?Thr?Pro?Lys?Asp

260 265 270

Gly?Pro?Val?Glu?Phe?His?Trp?Tyr?Ala?Ala?Glu?Glu?Ala?Gly?Leu?Leu

275 280 285

Gly?Ser?Gln?Ala?Ile?Ala?Arg?Tyr?Lys?Lys?Glu?Gln?Gly?Ala?Lys?Ile

290 295 300

Asp?Ala?Met?Met?Glu?Phe?Asp?Met?Thr?Ala?Phe?Ile?Ala?Arg?Asn?Ala

305 310 315 320

Thr?Glu?Thr?Ile?Gly?Phe?Val?Ala?Thr?Gln?Ala?Asp?Ala?Ala?Leu?Thr

325 330 335

Asn?Trp?Ala?Leu?Asn?Leu?Ser?Arg?Glu?Tyr?Ile?Ser?Ile?Pro?Ala?Glu

340 345 350

Val?Tyr?Glu?Leu?Gly?Pro?Asn?Ala?Gly?Ser?Asp?Tyr?Met?Ser?Tyr?Thr

355 360 365

Lys?Leu?Asn?Tyr?Pro?Ala?Ala?Phe?Ala?Ser?Glu?Gly?Asn?Pro?Leu?Ala

370 375 380

Gly?Gly?Ser?Phe?Pro?Gly?Glu?Met?Asp?Pro?Tyr?Val?His?Gly?Ile?Lys

385 390 395 400

Asp?Arg?Met?Asp?Val?Asp?Asp?Glu?Thr?Gly?Val?Phe?Ser?Ile?Glu?His

405 410 415

Met?Ala?Arg?Phe?Ser?Glu?Leu?Ala?Ile?Ala?Phe?Val?Val?Glu?Gln?Ala

420 425 430

Gly?Trp?Asp?Asn?Thr?Trp?Arg

435

<210>169

<211>526

<212>PRT

＜213〉aspergillus niger

<400>169

Met?Arg?Ser?Phe?Ser?Val?Val?Ala?Ala?Ala?Ser?Leu?Ala?Leu?Ser?Trp

1 5 10 15

Ala?Ser?Leu?Ala?Gln?Ala?Ala?Arg?Pro?Arg?Leu?Val?Pro?Lys?Pro?Ile

20 25 30

Ser?Arg?Pro?Ala?Ser?Ser?Lys?Ser?Ala?Ala?Thr?Thr?Gly?Glu?Ala?Tyr

35 40 45

Phe?Glu?Gln?Leu?Leu?Asp?His?His?Asn?Pro?Glu?Lys?Gly?Thr?Phe?Ser

50 55 60

Gln?Arg?Tyr?Trp?Trp?Ser?Thr?Glu?Tyr?Trp?Gly?Gly?Pro?Gly?Ser?Pro

65 70 75 80

Val?Val?Leu?Phe?Asn?Pro?Gly?Glu?Val?Ser?Ala?Asp?Gly?Tyr?Glu?Gly

85 90 95

Tyr?Leu?Thr?Asn?Asp?Thr?Leu?Thr?Gly?Val?Tyr?Ala?Gln?Glu?Ile?Gln

100 105 110

Gly?Ala?Val?Ile?Leu?Ile?Glu?His?Arg?Tyr?Trp?Gly?Asp?Ser?Ser?Pro

115 120 125

Tyr?Glu?Val?Leu?Asn?Ala?Glu?Thr?Leu?Gln?Tyr?Leu?Thr?Leu?Asp?Gln

130 135 140

Ser?Ile?Leu?Asp?Met?Thr?Tyr?Phe?Ala?Glu?Thr?Val?Lys?Leu?Gln?Phe

145 150 155 160

Asp?Asn?Ser?Ser?Arg?Ser?Asn?Ala?Gln?Asn?Ala?Pro?Trp?Val?Met?Val

165 170 175

Gly?Gly?Ser?Tyr?Ser?Gly?Ala?Leu?Thr?Ala?Trp?Thr?Glu?Ser?Ile?Ala

180 185 190

Pro?Gly?Thr?Phe?Trp?Ala?Tyr?His?Ala?Thr?Ser?Ala?Pro?Val?Glu?Ala

195 200 205

Ile?Tyr?Asp?Phe?Trp?Gln?Tyr?Phe?Tyr?Pro?Ile?Gln?Gln?Gly?Met?Ala

210 215 220

Gln?Asn?Cys?Ser?Lys?Asp?Val?Ser?Leu?Val?Ala?Glu?Tyr?Val?Asp?Lys

225 230 235 240

Ile?Gly?Lys?Asn?Gly?Thr?Ala?Lys?Glu?Gln?Gln?Glu?Leu?Lys?Glu?Leu

245 250 255

Phe?Gly?Leu?Gly?Ala?Val?Glu?His?Tyr?Asp?Asp?Phe?Ala?Ala?Val?Leu

260 265 270

Pro?Asn?Gly?Pro?Tyr?Leu?Trp?Gln?Asp?Asn?Asp?Phe?Val?Thr?Gly?Tyr

275 280 285

Ser?Ser?Phe?Phe?Gln?Phe?Cys?Asp?Ala?Val?Glu?Gly?Val?Glu?Ala?Gly

290 295 300

Ala?Ala?Val?Thr?Pro?Gly?Pro?Glu?Gly?Val?Gly?Leu?Glu?Lys?Ala?Leu

305 310 315 320

Ala?Asn?Tyr?Ala?Asn?Trp?Phe?Asn?Ser?Thr?Ile?Leu?Pro?Asn?Tyr?Cys

325 330 335

Ala?Ser?Tyr?Gly?Tyr?Trp?Thr?Asp?Glu?Trp?Ser?Val?Ala?Cys?Phe?Asp

340 345 350

Ser?Tyr?Asn?Ala?Ser?Ser?Pro?Ile?Phe?Thr?Asp?Thr?Ser?Val?Gly?Asn

355 360 365

Pro?Val?Asp?Arg?Gln?Trp?Glu?Trp?Phe?Leu?Cys?Asn?Glu?Pro?Phe?Phe

370 375 380

Trp?Trp?Gln?Asp?Gly?Ala?Pro?Glu?Gly?Thr?Ser?Thr?Ile?Val?Pro?Arg

385 390 395 400

Leu?Val?Ser?Ala?Ser?Tyr?Trp?Gln?Arg?Gln?Cys?Pro?Leu?Tyr?Phe?Pro

405 410 415

Glu?Val?Asn?Gly?Tyr?Thr?Tyr?Gly?Ser?Ala?Lys?Gly?Lys?Asn?Ser?Ala

420 425 430

Thr?Val?Asn?Ser?Trp?Thr?Gly?Gly?Trp?Asp?Met?Thr?Arg?Asn?Thr?Thr

435 440 445

Arg?Leu?Ile?Trp?Thr?Asn?Gly?Gln?Tyr?Asp?Pro?Trp?Arg?Asp?Ser?Gly

450 455 460

Val?Ser?Ser?Thr?Phe?Arg?Pro?Gly?Gly?Pro?Leu?Val?Ser?Thr?Ala?Asn

465 470 475 480

Glu?Pro?Val?Gln?Ile?Ile?Pro?Gly?Gly?Phe?His?Cys?Ser?Asp?Leu?Tyr

485 490 495

Met?Glu?Asp?Tyr?Tyr?Ala?Asn?Glu?Gly?Val?Arg?Lys?Val?Val?Asp?Asn

500 505 510

Glu?Val?Lys?Gln?Ile?Lys?Glu?Trp?Val?Glu?Glu?Tyr?Tyr?Ala

515 520 525

<210>170

<211>424

<212>PRT

＜213〉aspergillus niger

<400>170

Met?Gln?Leu?Leu?Gln?Ser?Leu?Ile?Val?Ala?Val?Cys?Phe?Ser?Tyr?Gly

1 5 10 15

Val?Leu?Ser?Leu?Pro?His?Gly?Pro?Ser?Asn?Gln?His?Lys?Ala?Arg?Ser

20 25 30

Phe?Lys?Val?Glu?Arg?Val?Arg?Arg?Gly?Thr?Gly?Ala?Leu?His?Gly?Pro

35 40 45

Ala?Ala?Leu?Arg?Lys?Ala?Tyr?Arg?Lys?Tyr?Gly?Ile?Ala?Pro?Ser?Ser

50 55 60

Phe?Asn?Ile?Asp?Leu?Ala?Asp?Phe?Lys?Pro?Ile?Thr?Thr?Thr?His?Ala

65 70 75 80

Ala?Ala?Gly?Ser?Glu?Ile?Ala?Glu?Pro?Asp?Gln?Thr?Gly?Ala?Val?Ser

85 90 95

Ala?Thr?Ser?Val?Glu?Asn?Asp?Ala?Glu?Phe?Val?Ser?Pro?Val?Leu?Ile

100 105 110

Gly?Gly?Gln?Lys?Ile?Val?Met?Thr?Phe?Asp?Thr?Gly?Ser?Ser?Asp?Phe

115 120 125

Trp?Val?Phe?Asp?Thr?Asn?Leu?Asn?Glu?Thr?Leu?Thr?Gly?His?Thr?Glu

130 135 140

Tyr?Asn?Pro?Ser?Asn?Ser?Ser?Thr?Phe?Lys?Lys?Met?Asp?Gly?Tyr?Thr

145 150 155 160

Phe?Asp?Val?Ser?Tyr?Gly?Asp?Asp?Ser?Tyr?Ala?Ser?Gly?Pro?Val?Gly

165 170 175

Thr?Asp?Thr?Val?Asn?Ile?Gly?Gly?Ala?Ile?Val?Lys?Glu?Gln?Ala?Phe

180 185 190

Gly?Val?Pro?Asp?Gln?Val?Ser?Gln?Ser?Phe?Ile?Glu?Asp?Thr?Asn?Ser

195 200 205

Asn?Gly?Leu?Val?Gly?Leu?Gly?Phe?Ser?Ser?Ile?Asn?Thr?Ile?Lys?Pro

210 215 220

Glu?Ala?Gln?Asp?Thr?Phe?Phe?Ala?Asn?Val?Ala?Pro?Ser?Leu?Asp?Glu

225 230 235 240

Pro?Val?Met?Thr?Ala?Ser?Leu?Lys?Ala?Asp?Gly?Val?Gly?Glu?Tyr?Glu

245 250 255

Phe?Gly?Thr?Ile?Asp?Lys?Asp?Lys?Tyr?Gln?Gly?Asn?Ile?Ala?Asn?Ile

260 265 270

Ser?Val?Asp?Ser?Ser?Asn?Gly?Tyr?Trp?Gln?Phe?Ser?Thr?Pro?Lys?Tyr

275 280 285

Ser?Val?Ala?Asp?Gly?Glu?Leu?Lys?Asp?Ile?Gly?Ser?Leu?Asn?Thr?Ser

290 295 300

Ile?Ala?Asp?Thr?Gly?Thr?Ser?Leu?Met?Leu?Leu?Asp?Glu?Asp?Val?Val

305 310 315 320

Thr?Ala?Tyr?Tyr?Ala?Gln?Val?Pro?Asn?Ser?Val?Tyr?Val?Ser?Ser?Ala

325 330 335

Gly?Gly?Tyr?Ile?Tyr?Pro?Cys?Asn?Thr?Thr?Leu?Pro?Ser?Phe?Ser?Leu

340 345 350

Val?Leu?Gly?Glu?Ser?Ser?Leu?Ala?Thr?Ile?Pro?Gly?Asn?Leu?Ile?Asn

355 360 365

Phe?Ser?Lys?Val?Gly?Thr?Asn?Thr?Thr?Thr?Gly?Gln?Ala?Leu?Cys?Phe

370 375 380

Gly?Gly?Ile?Gln?Ser?Asn?Gly?Asn?Thr?Ser?Leu?Gln?Ile?Leu?Gly?Asp

385 390 395 400

Ile?Phe?Leu?Lys?Ala?Phe?Phe?Val?Val?Phe?Asp?Met?Arg?Gly?Pro?Ser

405 410 415

Leu?Gly?Val?Ala?Ser?Pro?Lys?Asn

420

<210>171

<211>548

<212>PRT

＜213〉aspergillus niger

<400>171

Met?Arg?Ile?Asp?Ser?Ala?Ala?Leu?His?Leu?Val?Pro?Val?Leu?Leu?Gly

1 5 10 15

Gln?Val?Gly?Ala?Leu?Gln?Leu?Pro?Leu?Val?Gln?Asp?Ser?Asn?Ser?Gln

20 25 30

Trp?Gln?Lys?Pro?Asn?Ala?Gly?Asp?Lys?Pro?Leu?Ile?Ser?Ser?Pro?Leu

35 40 45

Leu?Gln?Glu?Gln?Val?Lys?Ala?Glu?Asn?Leu?Leu?Asp?Arg?Ala?Arg?Gln

50 55 60

Leu?Tyr?Lys?Ile?Ala?Glu?Leu?Gly?Glu?Asp?Glu?Tyr?Asn?His?Pro?Thr

65 70 75 80

Arg?Val?Ile?Gly?Ser?Lys?Gly?His?Leu?Gly?Thr?Leu?Asp?Tyr?Ile?Tyr

85 90 95

Ser?Thr?Leu?Thr?Asp?Leu?Gly?Asp?Tyr?Tyr?Thr?Val?Val?Asn?Gln?Ser

100 105 110

Phe?Pro?Ala?Val?Ser?Gly?Asn?Val?Phe?Glu?Ser?Arg?Leu?Val?Leu?Gly

115 120 125

His?Asp?Val?Pro?Lys?Ser?Ala?Thr?Pro?Met?Gly?Leu?Thr?Pro?Pro?Thr

130 135 140

Arg?Asn?Lys?Glu?Pro?Val?Tyr?Gly?Ser?Leu?Val?Ala?Val?Ser?Asn?Leu

145 150 155 160

Gly?Cys?Glu?Ala?Ser?Asp?Tyr?Ser?Ser?Asn?Leu?Lys?Gly?Ala?Val?Ala

165 170 175

Phe?Ile?Ser?Arg?Gly?Ser?Cys?Pro?Phe?Gly?Thr?Lys?Ser?Gln?Leu?Ala

180 185 190

Gly?Lys?Ala?Gly?Ala?Val?Ala?Ala?Val?Ile?Tyr?Asn?Asn?Glu?Arg?Gly

195 200 205

Asp?Leu?Ser?Gly?Thr?Leu?Gly?Asn?Pro?Thr?Pro?Asp?His?Val?Ala?Thr

210 215 220

Phe?Gly?Ile?Ser?Asp?Glu?Asp?Ala?Ala?Pro?Val?Leu?Glu?Lys?Leu?Asn

225 230 235 240

Lys?Gly?Glu?Lys?Val?Asp?Ala?Ile?Ala?Tyr?Val?Asp?Ala?Ile?Val?Glu

245 250 255

Thr?Ile?His?Thr?Thr?Asn?Ile?Ile?Ala?Gln?Thr?Thr?Asp?Gly?Asp?Pro

260 265 270

Asn?Asn?Cys?Val?Met?Leu?Gly?Gly?His?Ser?Asp?Ser?Val?Ala?Glu?Gly

275 280 285

Pro?Gly?Ile?Asn?Asp?Asp?Gly?Ser?Gly?Thr?Leu?Thr?Leu?Leu?Glu?Leu

290 295 300

Ala?Thr?Leu?Leu?Thr?Gln?Phe?Arg?Val?Asn?Asn?Cys?Val?Arg?Phe?Ala

305 310 315 320

Trp?Trp?Ala?Ala?Glu?Glu?Glu?Gly?Leu?Leu?Gly?Ser?Asp?Tyr?Tyr?Val

325 330 335

Ser?Val?Leu?Thr?Pro?Glu?Glu?Asn?Arg?Lys?Ile?Arg?Leu?Phe?Met?Asp

340 345 350

Tyr?Asp?Met?Leu?Gly?Ser?Pro?Asn?Phe?Ala?Tyr?Gln?Val?Tyr?Asn?Ala

355 360 365

Thr?Asn?Ala?Val?Asn?Pro?Glu?Gly?Ser?Glu?Glu?Leu?Arg?Asp?Leu?Tyr

370 375 380

Thr?Asp?Phe?Tyr?Glu?Asp?His?Gly?Phe?Asn?Tyr?Thr?Tyr?Ile?Pro?Phe

385 390 395 400

Asp?Gly?Arg?Ser?Asp?Tyr?Asp?Ala?Phe?Ile?Arg?His?Gly?Ile?Pro?Gly

405 410 415

Gly?Gly?Ile?Ala?Thr?Gly?Ala?Glu?Gly?Ile?Lys?Thr?Val?Glu?Glu?Ala

420 425 430

Asp?Met?Phe?Gly?Gly?Val?Ala?Gly?Gln?Trp?Tyr?Asp?Pro?Cys?Tyr?His

435 440 445

Gln?Ile?Cys?Asp?Thr?Val?Ala?Asn?Val?Asn?Leu?Thr?Ala?Trp?Glu?Trp

450 455 460

Asn?Thr?Lys?Leu?Val?Ala?His?Ser?Ile?Ala?Thr?Tyr?Ala?Lys?Ser?Phe

465 470 475 480

Asp?Gly?Phe?Pro?Glu?Arg?Ser?Asp?Glu?Pro?Ile?Ser?Pro?Ala?Ala?Phe

485 490 495

Glu?Glu?Pro?Lys?Tyr?His?Gly?His?Ala?Leu?Gln?Leu?Leu?Arg?Gly?Asn

500 505 510

Thr?Thr?Gly?Thr?Gln?Ser?Val?Leu?Trp?Gly?Ala?Gln?Ile?Gln?Asn?Gly

515 520 525

Thr?Ala?Ala?Ser?Val?Leu?Asn?Leu?Leu?Ser?Ile?Arg?Arg?Arg?Gly?Thr

530 535 540

Phe?Ser?Leu?Ser

545

Claims (28)

1. isolating polynucleotide, these polynucleotide can with hybridize according to the polynucleotide sequence that be selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or be selected from the sequence of SEQ ID NO:58～SEQ ID NO:114.

2. according to the isolating polynucleotide of claim 1, these polynucleotide under height stringency condition can with hybridize according to the polynucleotide that be selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or be selected from the sequence of SEQ ID NO:58～SEQ ID NO:114.

3. according to the isolating polynucleotide of claim 1 or 2, it can obtain from filamentous fungus.

4. according to the isolating polynucleotide of claim 3, it can obtain from aspergillus niger.

5. isolating polynucleotide, this polynucleotide encoding comprise according to the aminoacid sequence of the sequence that is selected from SEQ IDNO:115～SEQ ID NO:171 or the polypeptide of its function equivalent.

6. isolating polynucleotide, this polynucleotide encoding is according to being selected from SEQ IDNO:115～polypeptide of sequence of SEQ ID NO:171 or at least one functional domain of its function equivalent.

7. isolating polynucleotide, these polynucleotide comprise nucleotide sequence or its function equivalent that basis is selected from the sequence of SEQ ID NO:1～SEQ ID NO:57 or is selected from the sequence of SEQ ID NO:58～SEQ ID NO:114.

8. according to the sequence that is selected from SEQ ID NO:1～SEQ ID NO:57 or be selected from the isolating polynucleotide of the sequence of SEQID NO:58～SEQ ID NO:114.

9. comprise carrier according to the polynucleotide sequence of claim 1～8.

10. according to the carrier of claim 9, wherein should operationally be connected with the adjusting sequence according to the polynucleotide sequence of claim 1～8, this adjusting sequence is suitable for this polynucleotide sequence and expresses in appropriate host cell.

11. according to the carrier of claim 10, wherein said appropriate host cell is a filamentous fungus.

12. preparation is according to the polynucleotide of claim 1～8 or according to the method for the carrier of claim 9～11, this method comprises cultivates with these polynucleotide or this carrier transformed host cells and the step of separating these polynucleotide or this carrier in this host cell.

13. according to the polypeptide of sequence or its function equivalent that are selected from SEQ ID NO:115～SEQ ID NO:171.

14. according to the polypeptide of claim 13, it can obtain from aspergillus niger.

15. isolated polypeptide, this polypeptide can obtain by expressing in appropriate host cell such as aspergillus niger according to the polynucleotide of claim 1～8 or according to the carrier of claim 9～11.

16. comprise the recombinant protein enzyme of the functional domain of protease polypeptide.

17. preparation is according to the method for the polypeptide of claim 13～16, the method includes the steps of, promptly transform appropriate host cell with the isolating polynucleotide of claim 1～8 or according to the carrier of claim 9～11, make cultivate under the condition that these polynucleotide can be expressed this cell and optional from this cell or culture medium the purifying encoded polypeptide.

18. comprise according to the polynucleotide of claim 1～8 or according to the recombinant host cell of the carrier of claim 9～11.

19. express recombinant host cell according to the polypeptide of claim 13～16.

20. comprise the recombinant host cell of polynucleotide of the protease polypeptide of encoding function inactivation.

21. recombinant host cell, the wherein polynucleotide of proteins encoded enzyme polypeptide excalation at least.

22. according to the recombinant host cell of claim 18～21, wherein this host cell is from the Aspergillus species, as aspergillus niger.

23. have the recombinant host cell of protease function defective, this cell can obtain by the method that comprises this step:

A. external sudden change is according to the polynucleotide of claim 1～11,

B. the host cell that comprises native gene is transformed, this native gene comprises the polynucleotide sequence that can hybridize with the polynucleotide of the sudden change that obtains in step a),

C. select and separate recombinant host cell, wherein the polynucleotide of the sudden change that obtained in step a) of this native gene are replaced.

24. the antibody purified that can react with polypeptide according to claim 13～16.

25. comprise fused protein according to the peptide sequence of claim 13～16.

26. whether the diagnosis organism has infected the method for aspergillosis, this method comprises this step:

A. separating bio product that imitate from this doubtful biology that infects aspergillosis,

B. isolating nucleic acid from this sample,

C. determine this isolating nucleic acid whether comprise can with the polynucleotide according to the multi-nucleotide hybrid of claim 1～8.

27. according to the method for claim 26, wherein step c) comprises this isolating nucleic acid of amplification in addition, preferably passes through polymerase chain reaction.

28. diagnose certain organism whether to infect the method for aspergillosis, the method includes the steps of:

A. separating bio product that imitate from this doubtful biology that infects aspergillosis,

B. make this biological sample and react according to the antibody of claim 24,

C. determine whether to have formed immunocomplex.