CN101904986A - Compound aloetic bacteriostatic traditional Chinese medicine film and preparation method thereof - Google Patents
Compound aloetic bacteriostatic traditional Chinese medicine film and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound aloetic bacteriostatic traditional Chinese medicine film and a preparation method thereof. The compound aloetic bacteriostatic traditional Chinese medicine film comprises the following components in parts by weight: 15-25 parts of chitosan, 5-100 parts of traditional Chinese medicine effective constituents and 1-2 parts of glycerin, wherein the traditional Chinese medicine effective constituents include 28-55 parts of aloe extracting solutions, 20-40 parts of bletilla striata extracting solutions and 1-15 parts of radix ampelopsis extracting solutions. The compound aloetic bacteriostatic traditional Chinese medicine film has bacteriostatic effects of different degrees on staphylococcus aureus, staphylococcus epidermidis and colon bacillus, is prepared from natural pure traditional Chinese medicines, has the advantages of softness, transparency, safety, high hydrophilicity and ventilation property, no irritation, and the like, not only can resist bacteria and diminish inflammation, but also can eliminate swelling and promote granulation, thereby preventing the weakness of drug fastness caused by using antibiotics for a long time and being beneficial to healing a wound surface; in addition, the discloses a compound aloetic bacteriostatic traditional Chinese medicine film used as an adjuvant therapy medicine for ulcer, bedsore, skin or mucosa infection and minor operation incision infection on clinic has very well application prospect.
Description
Technical field
The invention belongs to the Chinese medicine preparation technical field, relate to a kind of compound aloetic bacteriostatic Chinese medicine film and preparation method thereof.
Background technology
Chinese herbal medicine is the rarity in China's medicine treasure-house, plays an important role in medical treatment, health service over thousands of years.Useful for a long time since ancient times medical herbs sticks, embathes the record of treatment various diseases.But heavy, the easy adhesion of homochromatics such as traditional Chinese medicine preparation such as plaster etc. are so Chinese medicine west system is present research focus.Confirm through clinical trial for many years: effectively Chinese medicine compound or single medicinal material can make things convenient for the patient to use by changing dosage form, have kept original curative effect again simultaneously, make the modern Chinese medicine novel form better be patient's service.
The Chinese medicine patch membrane is a kind of novel form of following the Chinese medicine preparation modernization to arise at the historic moment, the effective ingredient that it normally will extract from Chinese crude drug, and promptly Chinese medicine extract and filmogen are mixed and made into thin film.This medicine film can stick on wound or ulcer surfaces such as skin, mucosa, medicine directly acts on the affected part, avoid oral, the caused blood drug level peak valley phenomenon of drug administration by injection, reduced the toxicity of blood drug level peak period, improved bioavailability of medicament.Secondly because the effective ingredient of membranous patch directly enters in the body through the skin absorption, can avoid " first pass effect " and the gastrointestinal reaction of medicine, and easy to use.At present more about the report of Western medicine antibiolics film with slow-release function, but owing to human antibiotic dependency is increased year by year, for slowing down the speed that drug resistance produces, Ministry of Public Health clearly stipulates to avoid as much as possible the long-term local antibiotic that uses.And staphylococcus aureus, staphylococcus epidermidis and beta hemolytic streptococcus all belong to the Gram-positive pyococcus, the clinical just common pathogenic bacteria that causes mucocutaneous infections.Pathogenic cocci can be invaded body by number of ways, causes mucocutaneous, various histoorgan suppurative inflammation, even septicemia, pyemia etc.Staphylococcus aureus and escherichia coli still are common isolate during diabetic foot infects.Since penicillin used, staphylococcus produced drug resistance fast.At present, bacterial strain below 10% is only arranged to the penicillin sensitivity.Therefore, be badly in need of a kind of safety, hydrophilic is big, breathability is strong, non-stimulated middle medicine film clinically.By retrieval, adopt the Aloe of variable concentrations, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis extraction solution and chitosan are mixed with the herbal mixture film and do not see bibliographical information as yet.
Summary of the invention
First purpose of the present invention is to overcome Western medicine antibiolics film and is easy to generate chemical sproof shortcoming, and a kind of softness that adopts natural pure Chinese medicine to make, transparent, safety, hydrophilic is big, breathability is strong, non-stimulated compound aloetic bacteriostatic Chinese medicine film are provided.
Second purpose of the present invention has been to disclose the preparation method of compound aloetic bacteriostatic Chinese medicine film.
The 3rd purpose of the present invention has been to disclose the application of compound aloetic bacteriostatic Chinese medicine film aspect treatment Gram-negative, Gram-positive pyococcus medicine.For achieving the above object, the invention provides following technical scheme:
A kind of compound aloetic bacteriostatic Chinese medicine film, it is characterized in that by pharmaceutically active ingredient, 1-2 part glycerol in 15-25 part chitosan, 5-100 part by volume proportioning form; The raw material that described middle pharmaceutically active ingredient is pressed the row volume parts is made:
Aloe extraction solution: 28-55 part,
Rhizoma Bletillae extraction solution: 20-40 part,
Radix Ampelopsis extraction solution: 5-15 part;
Wherein said extracting solution is to adopt water to obtain through refining.
The raw material that the preferred middle pharmaceutically active ingredient of the present invention is pressed the row volume proportion is made: Aloe extraction solution 30-50 part, Rhizoma Bletillae extraction solution 25-40 part, Radix Ampelopsis extraction solution 8-15 part.
The raw material that another preferred middle pharmaceutically active ingredient of the present invention is pressed the row volume proportion is made: Aloe extraction solution 35-45 part, Rhizoma Bletillae extraction solution 28-38 part, Radix Ampelopsis extraction solution 8-13 part.
Chinese medicine film of the present invention, its every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04～0.05mm.
Compound aloetic bacteriostatic Chinese medicine film of the present invention is that extracting solution is mixed with chitosan, adds glycerol and makees plasticizer, and suitably coating liquid paraffin is done remover on polyester sheet, and 100 ℃ intermittently are laid on the polyester sheet the aseptic air-dry film forming of room temperature behind the steam sterilization.It is undertaken by following step:
(1) take by weighing 35～70g medicinal aloe, adding 1500-3000ml distilled water under 50 ℃ of water-baths, stirs, soaks and extracted in 1-3 hour, filters, and get Aloe extraction solution, or after adding decolorizing with activated carbon, 4 ℃ of preservations is standby;
(2) with after the Pseudobulbus Bletillae (Rhizoma Bletillae), the Radix Ampelopsis pulverizing, take by weighing 35～70g respectively, add 700-1300ml and 300-700ml distilled water, under 50 ℃ of water-baths, stir, soak and extracted the centrifugal 20min of 3000rpm 1-3 hour, get the top colloidal solution, make the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis extraction solution respectively, 4 ℃ of preservations are standby;
(3) take by weighing 7～13g chitosan, add 300-700ml 2% acetic acid, soak, be stirred to dissolving fully, the water white transparency viscous solution, it is standby to transfer to pH7;
(4) polyester sheet is taken out after alcohol-pickled 1 hour with 75%, place sterile petri dish, air-dry under the ultraviolet radiation;
(5) evenly smear sterile liquid paraffin and on polyester sheet, do remover;
(6) again with the extracting solution of (1)-(3) gained and glycerol mix homogeneously according to a certain ratio, 100 ℃ intermittently are laid on the polyester sheet behind the steam sterilization, and the aseptic air-dry film forming of room temperature is cut apart and vacuum-packed by different size, and every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04～0.05mm.
The present invention can adopt two kinds of methods to prepare compound Chinese aloe medicine film: a kind of effective ingredient that is water is carried Aloe, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis is directly made film.Another kind is after Aloe is extracted, and adds the activated carbon decolorizing of 3-5%, its objective is the color for the membrane that takes on a new look, and makes it colourless, transparent, and is convenient in use to the observation of wound surface, and still can keep its fungistatic effect preferably.
The present invention further discloses the application of Chinese medicine film aspect treatment gram negative bacteria, Gram-positive pyococcus medicine.Be included in the application of treatment staphylococcus aureus, staphylococcus epidermidis and escherichia coli medicine aspect.For example treat the application of the ancillary drug aspect of ulcer, decubital ulcer, skin or mucosal infections.Also comprise the ancillary drug that infects and prevent and treat the minor operation infection of incisional wound as the treatment diabetic foot.
The using method of compound aloetic bacteriostatic Chinese medicine film of the present invention is: at first clean wound surface, fractional pack is torn, taking-up medicine film sticks on wound surface and gets final product.As wound surface is the movability position, and available hospital gauze is fixed.
The inventor has taken into full account the technology of the selection and the preparation of prescription when developing compound aloetic bacteriostatic Chinese medicine film.When selecting prescription, all select the drug effect unanimity, and help film forming side's medicine.Have regulate hormone level, enhance immunity, anti-inflammation, antiviral, the promotion non-viable non-apoptotic cell comes off and promote effects such as wound healing as Aloe; Pseudobulbus Bletillae (Rhizoma Bletillae) glue has astringing to arrest bleeding, eliminating carbuncle to hold back skin ulcer, putrefaction-removing granulation-promoting and effect such as anticancer, human skin fibroblast to In vitro culture has tangible promotion growth effect, the record of hindering, scalding from ancient times with regard to useful its treatment rod, and itself also be a kind of filmogen of natural polymer preferably; Radix Ampelopsis has heat-clearing and toxic substances removing, granulation promoting, pain relieving, eliminating stagnation, antibiotic, antitumor, immunoregulatory effect; Passed through experiment repeatedly, the present invention has selected chitosan: 15-25 part, Aloe extraction solution: 28-55 part, Rhizoma Bletillae extraction solution: 20-40 part, Radix Ampelopsis extraction solution: 5-15 part, glycerol 1-2 part by volume proportioning are formed compound Chinese aloe medicine film.
The inventor has selected good biocompatibility, and the good natural medical polymer materials chitosan of degradability is as filmogen.That chitosan has is antibiotic, the effects such as regeneration, reparation and healing of antiinflammatory, hemostasis, promotion wound tissue, as a kind of novel film former, but prolong drug is in the holdup time of biological mucous membrane surface, strengthen the absorption of mucosa, promote the antibacterial and immunostimulating effect of antibacterials such as Aloe medicine.Regulate the ratio of the chitosan and the Pseudobulbus Bletillae (Rhizoma Bletillae), can control the speed of effective ingredient stripping, reach the effect of slow release.
The inventor utilizes the good characteristic of the medicinal aspect of Aloe, extract the effective ingredient in the Aloe, and compatibility other drug, with natural medical polymer materials as host material, prepare the compound percutaneous absorption Chinese medicine film that hydrophilic is big, breathability is strong, and the antagonistic property of medicine film has been carried out experimental study and clinical observation on the therapeutic effect.Thereby finished the application that suppresses staphylococcus aureus, staphylococcus epidermidis and escherichia coli aspect.Comprise ancillary drug, also comprise the ancillary drug that infects as the treatment diabetic foot as treatment ulcer, decubital ulcer, skin or mucosal infections and minor operation infection of incisional wound.Concrete bacteriostatic test is as follows:
1. instrument and material
1.1 instrument
Centrifuge (LDZ4-0.8 Beijing Medical Centrifugal Machine Factory), TN type pellet type torsion balance (Shanghai Second Balance Factory), electronic balance, 78HW-1 digital display constant temperature blender with magnetic force (Hangzhou motor for instrument company limited), other glass apparatus are homemade laboratory common instrument.
1.2 material
1.2.1 bacterial strain
Staphylococcus aureus (ATCC25923), staphylococcus aureus (ATCC29213), Pseudomonas aeruginosa (ATCC27853), escherichia coli (ATCC25922), staphylococcus epidermidis (ATCC 12228), proteus vulgaris (ATCC 49001), beta hemolytic streptococcus (ATCC 32204).More than provide by microbiology teaching and research room of Medical University Of Tianjin.
1.2.2 medicine
Medicinal aloe (Baoding Ji Kang Chinese crude drug Co., Ltd, Hebei, the place of production), the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Ampelopsis (all purchasing) in pharmacy, (Nankai University provides chitosan, deacetylation 80%), and active carbon (Shenyang City's reagent two factories, HG3-1290-80), adhesive bandage (the Bandaid board includes 0.50 milligram/sheet of benzalkonium chloride).
1.2.3 culture medium
Nutrient broth nutrient broth medium (Beijing extensive and profound in meaning star biotechnology responsibility company limited), agar powder (nutrient agar (China Import And Export Commodity Inspection Technology Institute, and Beijing overpass commodity inspection New Tech S. R. L. produces)), other reagent are homemade analytical pure.
2 experimental techniques
The preparation of Chinese medicine extraction liquid 2.1 respectively distinguish the flavor of
2.1.1 the preparation of Aloe extraction solution
Take by weighing medicinal aloe 70g adding 3000ml distilled water, under 50 ℃ of water-baths, stir, soak and extracted 1 hour, filter, get the dark-brown Aloe extraction solution, pH5,4 ℃ of preservations standby (no bleaching Aloe extraction solution).
Other gets it filled and uses Aloe 70g, with method operate the dark-brown Aloe extraction solution.Add the 0.8g active carbon in filtrate, boiled 4 minutes, filter, get the decolored aloe extracting solution, 4 ℃ of preservations are standby.
2.1.2 the preparation of Rhizoma Bletillae extraction solution
The Pseudobulbus Bletillae (Rhizoma Bletillae) takes by weighing 70g after pulverizing, and adds the 1300ml distilled water, stirs down, soaks and extracted 1 hour in 50 ℃ of water-baths, and the centrifugal 20min of 3000rpm gets top colloidal solution pH7, and 4 ℃ of preservations are standby, and residue discards.
2.1.3 the preparation of Radix Ampelopsis extraction solution
After Radix Ampelopsis is pulverized, take by weighing 70g, add the 700ml distilled water, with the operation of 2.1.2 method, 5～6,4 ℃ of preservations of colloidal solution pH are standby, residue discards.
2.1.4 the preparation of neutral chitosan
Take by weighing the 13g chitosan, add 700ml 2% acetic acid, soak, be stirred to dissolving fully, get the water white transparency viscous solution, pH4.It is standby to transfer to pH7 with NaOH.
2.2 the composition of compound Chinese aloe medicine film:
Aloe extraction solution: 28～55 parts; Rhizoma Bletillae extraction solution: 20～40 parts; Radix Ampelopsis extraction solution: 5～15 parts; Chitosan: 15～25 parts; Medical glycerol: 1～2 part.
The preparation method of compound Chinese aloe medicine film:
Polyester sheet is taken out after alcohol-pickled 1 hour with 75%, place sterile petri dish, air-dry under the ultraviolet radiation.Evenly smear sterile liquid paraffin and on polyester sheet, do remover.With extracting solution and the medical glycerol mix homogeneously of preparation, 100 ℃ intermittently behind the steam sterilization, be laid on the polyester sheet, the aseptic air-dry film forming of room temperature is cut apart and vacuum packaging by different size, and every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04～0.05mm.
2.3 medicine film bacteriostatic test
Intensive setting-out method is inoculated staphylococcus aureus (ATCC25923), staphylococcus aureus (ATCC29213), Pseudomonas aeruginosa (ATCC 27853), escherichia coli (ATCC25922), staphylococcus epidermidis (ATCC 12228), proteus vulgaris (ATCC 49001) respectively in plating medium, and beta hemolytic streptococcus (ATCC 32204) is inoculated in the blood plate culture medium.The medicine film is affixed on three identical bouillon agar plating mediums, and the different every flat boards of medicine film paste 2, amount to 6; Respectively with adhesive bandage and aseptic filter paper the sheet positive and negative control identical with medicine film size.In 37 ℃ of incubators, be inverted overnight incubation, the size of antibacterial ring around the measuring diaphragm.
3. statistical analysis
Experimental data is carried out the multisample one factor analysis of variance through the SPSS11.5 statistical software, relatively adopts SNK method (Student Newman Keula, q method of inspection) between two groups, and P＜0.05 thinks that difference has statistical significance.
4. result
The main component of kinds of traditional Chinese medicines such as this experiment employing water extraction extraction Aloe and the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis, carry out compatibility by a certain percentage, with good natural biological macromolecular material chitosan is main filmogen, make two kinds of Transdermal absorption Chinese medicine films respectively, observe relatively its fungistatic effect, the results are shown in Figure 1～Fig. 4 and table 1.
Two kinds of compound Chinese aloe medicines of table 1 film is to comparison (n=6) * of each strain fungistatic effect
*: filter paper does not all have antibacterial ring and occurs, and adds up so exclude in the table.
4.1 two kinds of compound Chinese aloe medicine films are to the comparison of same strain fungistatic effect
As shown in Table 1, two kinds of compound Chinese aloe medicine films all have in various degree bacteriostasis to staphylococcus epidermidis, staphylococcus aureus, escherichia coli, and two kinds of medicine films are not obvious to beta hemolytic streptococcus, proteus vulgaris, Pseudomonas aeruginosa fungistatic effect.Adhesive bandage is only to Pseudomonas aeruginosa unrestraint phenomenon, and other strains are all had the inhibition phenomenon.
As seen utilization SNK method compares between organizing in twos, two kinds of compound Chinese aloe medicine films are to the fungistatic effect difference with insignificance (the P value is all greater than 0.05) of staphylococcus epidermidis (ATCC12228), staphylococcus aureus (ATCC29213), escherichia coli (ATCC25922), no bleaching compound Chinese aloe medicine film has the trend greater than decolouring compound Chinese aloe medicine film, and both all are lower than the fungistatic effect (the P value is all less than 0.05) of adhesive bandage.Wherein, two kinds of compound Chinese aloe medicine films are to the fungistatic effect difference of staphylococcus aureus (ATCC25923), the fungistatic effect of the no bleaching compound Chinese aloe medicine film compound Chinese aloe medicine film (P＜0.05) that is better than decolouring, and no bleaching compound Chinese aloe medicine film and adhesive bandage fungistatic effect difference with insignificance (P＞0.05).
4.2 two kinds of compound Chinese aloe medicine films are to the comparison of different strain fungistatic effect
As shown in Table 1, no bleaching compound Chinese aloe medicine film is to the fungistatic effect of staphylococcus epidermidis and staphylococcus aureus similar (the P value is all greater than 0.05), and colibacillary fungistatic effect is lower than fungistatic effect (the P value is all less than 0.05) to staphylococcus epidermidis and staphylococcus aureus.
Decolour compound Chinese aloe medicine film to staphylococcus aureus identical with colibacillary fungistatic effect (the P value is all greater than 0.05), and the staphylococcic fungistatic effect of epidermis is lower than staphylococcus aureus and colibacillary fungistatic effect (the P value is all less than 0.05).
Adhesive bandage is to staphylococcus epidermidis, staphylococcus aureus (ATCC29213, ATCC25923), escherichia coli fungistatic effect identical (P＞0.05), fungistatic effect to beta hemolytic streptococcus, proteus vulgaris identical (P＞0.05), and staphylococcus epidermidis, staphylococcus aureus (ATCC29213, ATCC25923), colibacillary fungistatic effect be better than the fungistatic effect to beta hemolytic streptococcus and proteus vulgaris.
5. discuss
Compound Chinese aloe medicine film has good bacteriostasis for staphylococcus epidermidis, staphylococcus aureus and escherichia coli, therefore we can be in the auxiliary treatment that is applied to skin soft-tissue infection, suppurative wound, decubital ulcer etc., can also be with its local application as the diabetic foot treatment of infection, play control infection, the effect that reduce amputation, disables, causes death etc.Experimental result shows, compound Chinese aloe medicine film has good bacteriostasis to staphylococcus epidermidis, staphylococcus aureus and escherichia coli, its possible mechanism is the metabolism that main component aloe-emodin contained in the Aloe etc. has suppressed antibacterial, and the adding of the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis, chitosan has collaborative bacteriostasis.
The inventor is an example with embodiment 4, and prepared compound Chinese aloe medicine film has been carried out swelling, permeability test, mainly comprises:
(1) film thickness determination: 6 medicine films of picked at random, on each film to be measured, survey 6 groups of data, results averaged at random with thickness measuring instrument.0.042±0.004(mm)。
(2) swelling is measured with dissolubility: get 6 medicine films that thickness is even, equate, diaphragm is cut into 3 (mm) * 3 (mm) sizes, put into the distilled water of 37 ℃ of 10ml, submerging from diaphragm picks up counting, and observes diaphragm-operated swelling, course of dissolution continuously.The result is as follows:
First required time of swelling: 2.33 ± 0.49 (s)
Volume swells and is 1.5 times of required times of original volume: 8.98 ± 1.71 (s)
Volume swells and is 2 times of required times of original volume: 23.38 ± 4.74 (s)
Abundant swelling (being that volume no longer increases) required time: 93.30 ± 8.82 (s)
(3) the water droplet time of penetration is measured: get 6 of the identical medicine films of thickness respectively, splash into the equivalent distilled water at every medicine center membrane, to the time of leaking, be designated as water droplet time of penetration (Ts) is 16 ± 0.82min to record film by water saturates.
Compound Chinese aloe medicine film of the present invention has also carried out irritation test simultaneously: adopt with a mouse back both sides, unhairing, one side causes the wound back and pastes this compound Chinese aloe medicine film, also pastes this compound Chinese aloe medicine film in the opposite side normal skin simultaneously, and the local normal skin in next door is done contrast.The response situation of mouse skin wound and intact skin behind the observation subsides medicine film.The result: wound that compound Chinese aloe medicine film infects mouse skin and intact skin and skin of back are not found local edema and obvious erythematous response, compare no significant difference with the skin of own control one side.Illustrate that this product does not have zest to mucosa.
Compound Chinese aloe Chinese medicine film of the present invention is compared the good effect that is had with the bacteriostatic film of present use and is:
(1) the present invention chooses the mutual compatibility of Aloe, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis extraction solution with many-sided effect and forms.Though flavour of a drug less but curative effect is obvious, can effectively suppress common infective pathogen bacterium such as staphylococcus aureus, staphylococcus epidermidis and escherichia coli, are a kind of novel forms of following the Chinese medicine preparation modernization to arise at the historic moment.
(2) after compound aloetic bacteriostatic Chinese medicine film of the present invention was affixed on wound surface, effective ingredient can rapidly, directly act on affected part, Transdermal absorption.Avoid the GI irritation and the first pass effect of medicine, improved bioavailability of medicament.Simultaneously, because of medicine with host material mix built in, effective ingredient is embedded on the 3 D stereo polysaccharide structures skeleton of chitosan, with after wounds such as skin, mucosa, ulcer spot contact, can be when absorbing wound fluid, slowly discharge effective ingredient, duration of efficacy is longer.
(3) compound aloetic bacteriostatic Chinese medicine film thickness of the present invention only is 0.04-0.05mm, have softness, toughness is strong, the characteristics that compliance is good, easily stick can make wound surface air-isolation effectively, prevent and avoid infecting of airborne antibacterial, can absorb wound fluid simultaneously; Colourless, transparent, be convenient in therapeutic process, observe at any time the affected part, easily peel off replacing simultaneously, the patient is easy to accept.
(4) this medicine film is that natural pure Chinese medicine is made, have good characteristics such as safe, non-stimulated, mutual compatibility between the multi-flavor medicine, synergism, not only can anti-inflammation, but detumescence and promoting granulation has also been avoided owing to the life-time service antibiotic produces chemical sproof weakness, help the healing of wound surface, auxiliary clinical treatment.
Description of drawings:
Fig. 1 is the antibacterial situation of compound Chinese aloe medicine film to staphylococcus epidermidis (ATCC 12228), and wherein A is a no bleaching compound Chinese aloe medicine film; B is decolouring compound Chinese aloe medicine film;
Fig. 2 is the antibacterial situation of compound Chinese aloe medicine film to staphylococcus aureus (ATCC25923), and wherein A is a no bleaching compound Chinese aloe medicine film; B is decolouring compound Chinese aloe medicine film;
Fig. 3 is the antibacterial situation of compound Chinese aloe medicine film to staphylococcus aureus (ATCC29213), and wherein A is a no bleaching compound Chinese aloe medicine film; B is decolouring compound Chinese aloe medicine film;
Fig. 4 is the antibacterial situation of compound Chinese aloe medicine film to escherichia coli (ATCC 25922), and wherein A is a no bleaching compound Chinese aloe medicine film; B is decolouring compound Chinese aloe medicine film.
The specific embodiment:
For simple and purpose clearly, hereinafter appropriate omission the description of known technology, in order to avoid those unnecessary details influences are to the description of the technical program.The present invention is described further below in conjunction with example.Each raw material that is adopted in the composition is by commercially available.Umber wherein both can be a milliliter, rose; Also can be gram or kilogram, still can be weight, the simultaneous situation of volume, specifically selected according to the needs of producing.
Embodiment 1
(1) respectively the distinguish the flavor of preparation of Chinese medicine extraction liquid:
The preparation of Aloe extraction solution
Take by weighing medicinal aloe 65g, adding 2500ml distilled water under 50 ℃ of water-baths, stirs, soaks and extracted 2 hours, filters, and gets the dark-brown Aloe extraction solution, pH5, and 4 ℃ of preservations are standby.
Other gets it filled and uses Aloe 65g, with method operate the dark-brown Aloe extraction solution.Add the 8g active carbon in filtrate, boiled 4～5 minutes, filter, get the decolored aloe extracting solution, 4 ℃ of preservations are standby.
The preparation of Rhizoma Bletillae extraction solution
After the Pseudobulbus Bletillae (Rhizoma Bletillae) is pulverized, take by weighing 60g, add the 1000ml distilled water, stir down, soak and extracted 2 hours in 50 ℃ of water-baths, the centrifugal 20min of 3000rpm, it is standby to get 7,4 ℃ of preservations of top colloidal solution pH, and residue discards.
The preparation of Radix Ampelopsis extraction solution
After Radix Ampelopsis is pulverized, take by weighing 75g, add the 500ml distilled water, stir down, soak and extracted 3 hours in 50 ℃ of water-baths, the centrifugal 20min of 3000rpm, colloidal solution pH5～6,4 ℃ preservation is standby, residue discards.
The preparation of neutral chitosan
Take by weighing the 10g chitosan, add 500ml 2% acetic acid, soak, be stirred to dissolving fully, get the water white transparency viscous solution, pH4.It is standby to transfer to pH7 with NaOH.
(2) preparation method of compound Chinese aloe medicine film:
Polyester sheet is taken out after alcohol-pickled 1 hour with 75%, place sterile petri dish, air-dry under the ultraviolet radiation.Evenly smear sterile liquid paraffin and on polyester sheet, do remover.With the extracting solution and the glycerol mix homogeneously according to a certain ratio of preparation, 100 ℃ intermittently are laid on the polyester sheet behind the steam sterilization again, and the aseptic air-dry film forming of room temperature is cut apart and vacuum-packed by different size, and every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04mm.
Embodiment 2
30 parts of Aloe extraction solution, 20 parts of Rhizoma Bletillae extraction solution, 8 parts of Radix Ampelopsis extraction solution; 15 parts of chitosans, 2 parts of glycerol.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.18mL/cm
2, thickness is 0.05mm.
Embodiment 3
2 parts of 20 parts of chitosans, glycerol, 40 parts of Aloe extraction solution (decolouring), 30 parts of Rhizoma Bletillae extraction solution, 10 parts of Radix Ampelopsis extraction solution.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.25mL/cm
2, thickness is 0.04mm.
Embodiment 4
45 parts of Aloe extraction solution, 34 parts of Rhizoma Bletillae extraction solution, 11 parts of Radix Ampelopsis extraction solution, 23 parts of chitosans, 2 parts of glycerol.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.19mL/cm
2, thickness is 0.04mm.
Embodiment 5
50 parts of Aloe extraction solution, 32 parts of Rhizoma Bletillae extraction solution, Radix Ampelopsis extraction solution contains for 12 parts, 20 parts of chitosans, 2 parts of glycerol.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.20mL/cm
2, thickness is 0.04mm.
Embodiment 6
35 parts of Aloe extraction solution, 26 parts of Rhizoma Bletillae extraction solution, 10 parts of Radix Ampelopsis extraction solution, 20 parts of chitosans, 1 part of glycerol.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.22mL/cm
2, thickness is 0.05mm.
Embodiment 7
55 parts of Aloe extraction solution, 40 parts of Rhizoma Bletillae extraction solution, 15 parts of Radix Ampelopsis extraction solution, 25 parts of chitosans, 1 part of glycerol.Each composition by volume umber is formed.The preparation method of compound Chinese aloe medicine film is with embodiment 1.Every medicine film content of dispersion is 0.22mL/cm
2, thickness is 0.04mm.
Embodiment 8
Be compound Chinese aloe medicine film below, the clinical typical case aspect treatment decubital ulcer, diabetic foot infection, skin infection.
1. the skin infection due to boiled water is scalded:
Zhao * *, the man, 12 years old, to scald because of boiled water, blister is broken, the back skin infection that gets wet, redness.Wound surface cleaning is clean, wipe out vesicle and pus bubble skin after, compound Chinese aloe medicine film is affixed on wound surface open and flatly.Every day 2 times, transudate obviously reduces behind the pad pasting, 5 days decrustation.Patient's subjective symptoms is good, and stingless excitation is through the Preliminary Clinical Observation satisfactory effect.
2. the auxiliary treatment that infects of diabetic foot
53 years old Mrs Cheng suffers from diabetes, and left toes ulcer was suppurated 5 days, went to see a doctor, when taking antidiabetic drug, paste compound Chinese aloe medicine film every day 2 times, the ulcer symptom is clearly better after the week, held on for 2 weeks, and cooperate the other drug treatment, toes ulcer symptom fades away.
3. the auxiliary treatment of decubital ulcer
Patient Lee XX, the man, 75 years old, because of hemiplegia, long-term bed caused decubital ulcer, the sepage of skin ulceration.Therapeutic Method: every day debridement face, paste compound Chinese aloe medicine film, paste every day 2 times, roasting light irradiation 2 times, the skin ulcer face all was clearly better in the 5th day, dwindled, did not have and ooze out.Continue to paste compound Chinese aloe medicine film, the skin ulcer face healed substantially in the 10th day.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, every foundation technical spirit of the present invention all still belongs to category of the present invention to any simple modification, equivalent variations and modification that above embodiment did.
Claims (10)
1. compound aloetic bacteriostatic Chinese medicine film, it is characterized in that by pharmaceutically active ingredient, 1-2 part glycerol in 15-25 part chitosan, 5-100 part by volume proportioning form; The raw material that described middle pharmaceutically active ingredient is pressed the row volume proportion is made:
Aloe extraction solution: 28-55 part,
Rhizoma Bletillae extraction solution: 20-40 part,
Radix Ampelopsis extraction solution: 5-15 part;
Wherein said extracting solution is to adopt water to obtain through refining.
2. Chinese medicine film as claimed in claim 1, the raw material that its described middle pharmaceutically active ingredient is pressed the row volume proportion is made: Aloe extraction solution 30-50 part, Rhizoma Bletillae extraction solution 25-40 part, Radix Ampelopsis extraction solution 8-15 part.
3. Chinese medicine film as claimed in claim 1, the raw material that its described middle pharmaceutically active ingredient is pressed the row volume proportion is made: Aloe extraction solution 35-45 part, Rhizoma Bletillae extraction solution 28-38 part, Radix Ampelopsis extraction solution 8-13 part.
4. Chinese medicine film as claimed in claim 1, wherein every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04～0.05mm.
5. the preparation method of each described compound aloetic bacteriostatic Chinese medicine film of claim 1-4 is characterized in that being undertaken by following step:
(1) take by weighing 35～70g medicinal aloe, the distilled water of adding 1500-3000ml under 50 ℃ of water-baths, stirs, soaks and extracted 1-3 hour, filters, and after getting Aloe extraction solution or adding decolorizing with activated carbon, 4 ℃ of preservations are standby;
(2) with after the Pseudobulbus Bletillae (Rhizoma Bletillae), the Radix Ampelopsis pulverizing, take by weighing 35～70g respectively, add 700-1300ml and 300-700ml distilled water, stir down, soak and extracted 1-3 hour in 50 ℃ of water-baths, the centrifugal 20min of 3000rpm gets the top colloidal solution, make the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis extraction solution respectively, 4 ℃ of preservations are standby;
(3) take by weighing 7～13g chitosan, add 300-700ml 2% acetic acid, soak, be stirred to dissolving fully, the water white transparency viscous solution, it is standby to transfer to pH 7;
(4) polyester sheet is taken out after alcohol-pickled 1 hour with 75%, place sterile petri dish, air-dry under the ultraviolet radiation;
(5) evenly smear sterile liquid paraffin and on polyester sheet, do remover;
(6) again with the extracting solution of (1)-(3) gained and glycerol mix homogeneously according to a certain ratio, 100 ℃ intermittently are laid on the polyester sheet behind the steam sterilization, and the aseptic air-dry film forming of room temperature is cut apart and vacuum-packed by different size, and every medicine film content of dispersion is 0.18-0.25mL/cm
2, thickness is 0.04～0.05mm.
6. the application of each described Chinese medicine film of claim 1-4 aspect preparation treatment gram negative bacteria, Gram-positive pyococcus medicine.
7. the application of each described Chinese medicine film of claim 1-4 aspect preparation treatment staphylococcus aureus, staphylococcus epidermidis and escherichia coli medicine.
8. as claim 5 or 6 described application, comprise application as the ancillary drug aspect of preparation treatment ulcer, decubital ulcer, skin or mucosal infections.
9. as claim 5 or 6 described application, comprise the application of the ancillary drug aspect that infects as preparation treatment diabetic foot.
10. as claim 5 or 6 described application, comprise application as the healing aspect of preparation auxiliary treatment minor operation otch.
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| CN2009100691140A CN101904986B (en) | 2009-06-04 | 2009-06-04 | Compound aloetic bacteriostatic traditional Chinese medicine film and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107802618A (en) * | 2017-11-09 | 2018-03-16 | 福州大学 | A kind of preparation method of two-phase mixtures dual-use function high molecular slow-release film |
| CN108096419A (en) * | 2018-01-02 | 2018-06-01 | 南京伯朗生物科技有限公司 | Antiallergy medicine film and preparation method thereof |
| CN109706744A (en) * | 2018-12-03 | 2019-05-03 | 广州润虹医药科技股份有限公司 | A kind of quick-acting haemostatic powder, antibacterial hydrocolloid oil yarn and preparation method thereof |
| CN112336826A (en) * | 2020-12-04 | 2021-02-09 | 中国热带农业科学院农产品加工研究所 | A kind of liquid film preparation for treating otitis externa and preparation method thereof |
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2009
- 2009-06-04 CN CN2009100691140A patent/CN101904986B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107802618A (en) * | 2017-11-09 | 2018-03-16 | 福州大学 | A kind of preparation method of two-phase mixtures dual-use function high molecular slow-release film |
| CN108096419A (en) * | 2018-01-02 | 2018-06-01 | 南京伯朗生物科技有限公司 | Antiallergy medicine film and preparation method thereof |
| CN109706744A (en) * | 2018-12-03 | 2019-05-03 | 广州润虹医药科技股份有限公司 | A kind of quick-acting haemostatic powder, antibacterial hydrocolloid oil yarn and preparation method thereof |
| CN112336826A (en) * | 2020-12-04 | 2021-02-09 | 中国热带农业科学院农产品加工研究所 | A kind of liquid film preparation for treating otitis externa and preparation method thereof |
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| CN101904986B (en) | 2012-01-04 |
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