CN101865911A - A urine creatinine quantitative detection card - Google Patents

Abstract

The invention relates to a urine creatine quantitative test card, which comprises an upper shell, a lower shell, reaction test paper and developing test paper arranged in shell cavity, a pipette and a quantitative absorbing rod. The upper shell is provided with a sample loading hole; a solution tank is arranged in the shell; and the tail end of the reaction test paper is positioned in the solution tank. The urine creatine quantitative test card can realize instantaneous measurement of creatine in urine by combining simple and convenient urine quantitative dilution, enzyme reaction and chromatography developing technology, and has the advantages of simple structure, no need of mating instruments, convenient use, low cost, and accordance with application trend of point-of-care testing (POCT).

Description

A urine creatinine quantitative detection card

technical field

The invention relates to a urine detection card, in particular to a urine creatinine quantitative detection card for measuring creatinine concentration in urine.

Background technique

Creatinine is a product of muscle metabolism in the human body, and every 20g of muscle metabolism can produce 1mg of creatinine. In muscle, creatine is mainly slowly formed into creatinine through irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. Creatinine is mainly filtered out of the body by the glomerulus (an important part of the kidney). Blood creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat food metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. When the intake of meat food is stable and the body's muscle metabolism does not change significantly, the production of creatinine will be relatively constant.

Creatinine is a small molecular substance that can be filtered through the glomerulus and is rarely absorbed in the renal tubules. Almost all creatinine produced in the body is excreted with urine and is generally not affected by urine output. When renal insufficiency occurs, creatinine accumulates in the body and becomes a toxin harmful to the human body. A urine creatinine test measures the amount of creatinine in the blood that is filtered out by the kidneys. Its increase can be seen in acromegaly, gigantism, diabetes, infection, hypothyroidism, eating meat, exercise, intake of drugs (such as vitamin C, levodopa, methyldopa, etc.); urinary creatinine decreases in acute Or chronic renal insufficiency, severe congestive heart failure, hyperthyroidism, anemia, muscular dystrophy, leukemia, vegetarians, and taking androgen, thiazide drugs, etc. Therefore, detection of changes in urine creatinine content has important reference value for disease diagnosis, observation of disease changes and treatment effects. In recent years, urine creatinine analysis test paper has appeared, such as the Chinese patent application number 200910067221.X, which discloses a creatinine urine analysis test paper and its preparation method, using 2M sodium hydroxide buffer solution, pH value 10.5-12.5, 1000mL, chlorinated Sodium or potassium chloride 2g-5g, polyvinylpyrrolidone 1g-7g, orange G 100mg-200mg; 3,5-dinitrobenzoic acid 400mg-800mg, methanol 1000ml. However, this type of test paper can only detect urine creatinine semi-quantitatively, and cannot meet the quantitative detection requirements.

Contents of the invention

The technical problem to be solved by the present invention is to provide a urine creatinine quantitative detection card for measuring creatinine content in urine by combining enzymatic method and chromatography color development.

The technical problem adopted by the present invention to solve the technical problem is: a urine creatinine quantitative detection card, comprising: an upper housing, a lower housing, a reaction test paper and a color test paper placed in the housing chamber, a quantitative straw and a quantitative absorption Rod; the upper casing is provided with a sample hole, the casing is provided with a solution tank, and the end of the reaction test paper is located in the solution tank.

The reaction test paper contains creatinase, creatinase, sarcosine oxidase, horseradish peroxidase, wherein creatinase content ≥ 9U/cm ² , creatinase content ≥ 20U/cm ² , sarcosine oxidation Enzyme content ≥ 3U/cm ² , horseradish peroxidase ≥ 3U/cm ² .

For the convenience of volume calculation, the total adsorption volume of the reaction test paper and the color test paper is x, then the quantitative absorption volume of the quantitative pipette is a=x*72/50, and the quantitative absorption volume of the quantitative absorption rod is a=x*3/50.

For the convenience of observation, the upper housing above the color test paper is provided with an observation window, and the observation window is marked with a scale line and a scale mark. An end indicator hole is provided near the top of the upper casing, and the color-developing test paper below the indicator hole contains a water-soluble end point indicator.

In order to facilitate the absorption of water and easy operation, the quantitative absorption rod adopts a hydrophilic porous material; one end of the quantitative absorption rod is inserted into a hydrophobic material to form a hand-held end; the diameter of the quantitative absorption rod is smaller than the caliber of the quantitative straw. The length of the absorbent rod is less than the length of the dosing straw.

Due to the use of the above-mentioned technical solutions, the present invention has the following advantages compared with the prior art:

1. The present invention combines simple urine quantitative dilution, enzyme reaction and chromatographic technology, so it can realize instant determination of creatinine in urine.

2. The present invention is simple in structure, does not need to be used with supporting equipment, is convenient to use, and has low cost, and conforms to the application trend of POCT (point of care testing, instant detection).

Description of drawings

Fig. 1 is a schematic structural view of Embodiment 1 of the present invention;

Fig. 2 is the rear view of Embodiment 1 of the present invention;

Fig. 3 is a side view of Embodiment 1 of the present invention;

Fig. 4 is the front view of Embodiment 1 of the present invention;

Figure 5 is a sectional view of Embodiment 1 of the present invention;

Fig. 6 is a schematic structural view of Embodiment 1 of the present invention after removing the upper cover;

Fig. 7 is a schematic diagram of the quantitative absorption stick of the present invention;

Fig. 8 is the schematic diagram of quantitative straw of the present invention;

Fig. 9 is a graph showing the detection results of urine creatinine in the present invention.

1. Upper housing 2. Sample injection hole 3. Observation window 4. End indicator light 5. Lower housing

6. Color test paper 7. Reaction test paper 8. Solution tank 9. Hand-held terminal 10. Suction tube

11. Quantitative absorption stick 12. Quantitative straw

Detailed ways

The present invention will be further described below in conjunction with accompanying drawing and embodiment:

Embodiment one

As shown in Figure 1, a urine creatinine quantitative detection card includes: an upper housing 1, a lower housing 5, a reaction test paper 7 and a color development test paper 6 sleeved in the housing chamber, a quantitative suction pipe 12 and a quantitative absorption rod 11; The upper housing 1 is provided with a sampling hole 2, and the housing is provided with a solution tank 8, and the end of the reaction test paper 7 is located in the solution tank 8; the reaction test paper 7 includes creatinase, creatinase, sarcosine Acid oxidase, horseradish peroxidase.

The total adsorption volume of the reaction test paper 7 and the color test paper 6 is 250 uL; the quantitative absorption rod 11 used is a hydrophilic porous material glass fiber with a quantitative absorption volume of 15 uL, and one end of the quantitative absorption rod 11 is inserted into a hydrophobic material to form a hand-held end 9; the solution absorbed quantitatively by the quantitative pipette 12 is 360uL pH6.5 phosphate buffer; The upper housing 1 above the color test paper 6 is provided with an observation window 3, and the observation window 3 is marked with a scale line and has a scale mark; the upper housing 1 is provided with an end indication hole 4 near the top, indicating The color test paper 6 below the hole contains a water-soluble end point indicator. After the color development of the color test paper 6 is completed, the remaining solution is chromatographed to the end indication hole 4, and the color of the indicator will appear at the end indication hole 4, indicating that the detection has been completed. Finished and ready for result reading.

The preparation method of the reaction test paper 7 is as follows: preparing a solution according to the formula in the table below, dipping the test paper into the enzyme solution, and drying at 40° C. after repeated dipping.

In the above technical scheme, the preparation method of color test paper 6 is as follows: use hydrogen bromide or CDI to activate the carrier, the reagent to be covalently immobilized is preferably N-alkylaniline substances, and the reagent does not diffuse with the developing solution after fixation, and the concentration is preferably 2 -20mmol/L. The chromogenic system also includes another component: benzothiazolone hydrazones, preferably at a concentration of 0.01-1 mg/mL.

The working process of the above-mentioned urine creatinine quantitative detection card is as follows: use the quantitative absorption stick 11 to suck up the urine; tear off the mouth of the quantitative straw 12, put the quantitative absorption stick 11 into the quantitative straw 12, turn the straw upside down and press the mouth of the mouth Mix evenly; add all the solution in the straw from the sample hole 2 into the solution tank 8, and the reaction test paper 7 will chromatograph the solution to the color test paper 6 through capillary action; The height of the band indicates the concentration of creatinine in the urine.

The method of using the above urine creatinine quantitative test card is as follows:

① Place the test card horizontally, and use the quantitative absorption stick 11 to absorb the urine;

②Tear off the nozzle of the quantitative straw 12, put the quantitative absorption rod 11 into the quantitative straw 12, invert the straw and press the nozzle to mix evenly

③Put all the solution in the straw into the solution tank 8 from the sample injection hole 2 to start the reaction;

④Wait for 10-12 minutes, when the indicator in the end indicator hole 4 turns green, the reaction ends;

⑤ Read out the height of color development, and find out the corresponding urine creatinine concentration in the result comparison table.

The urine creatinine test results are shown in the table below and Figure 7:

Claims (8)

1. A urine creatinine quantitative detection card, comprising: an upper housing (1), a lower housing (5), a reaction test paper (7) placed in the housing chamber, a color test paper (6), a quantitative straw (12 ) and quantitative absorption stick (11), it is characterized in that, the upper housing (1) is provided with sample injection hole (2), is provided with solution tank (8) in the housing, and the end of described reaction test paper (7) is positioned at solution tank (8) inside. 2. according to the described urine creatinine quantitative detection card of claim 1, it is characterized in that, described reaction test paper (7) comprises creatinase, creatinase, sarcosine oxidase, horseradish peroxidase, wherein creatinine Enzyme content ≥ 9U/cm ² , creatinase content ≥ 20U/cm ² , sarcosine oxidase content ≥ 3U/cm ² , horseradish peroxidase ≥ 3U/cm ² . 3. according to the described urine creatinine quantitative detection card of claim 1, it is characterized in that, the total adsorption volume of described reaction test paper (7) and color development test paper (6) is x, then quantitative suction pipe (12) quantitative absorption volume is a =x*72/50, the quantitative absorption volume of the quantitative absorption rod (11) is a=x*3/50. 4. according to the described urine creatinine quantitative detection card of claim 1, it is characterized in that, the upper shell (1) above described chromogenic test paper (6) is provided with observation window (3), and observation window (3) superscript There are scale marks and scale marks. 5. The urine creatinine quantitative detection card according to claim 1, characterized in that, the upper housing (1) is provided with an end indication hole (4) near the top end, and the color-developing test paper below the end indication hole (4) (6) Contains a water-soluble endpoint indicator. 6. The urine creatinine quantitative detection card according to claim 1, characterized in that, the quantitative absorption stick (11) adopts a hydrophilic porous material. 7. The urine creatinine quantitative detection card according to claim 1, characterized in that one end of the quantitative absorption rod (11) is inserted into a hydrophobic material to form a hand-held end (9). 8. urine creatinine quantitative detection card according to claim 1, is characterized in that, the diameter of described quantitative absorption stick (11) is less than the aperture of quantitative straw (12), and the length of quantitative absorption stick (11) is less than quantitative straw ( 12) Length.

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