CN101849889B - Fragrance-lasting natural essential oil nanoemulsion used for cosmetology and SPA (essential oil therapy) and preparation method thereof - Google Patents

Abstract

The present invention relates to a natural essential oil nanoemulsion used for cosmetology and essential oil therapy and a preparation method thereof. The natural essential oil nanoemulsion for the cosmetology and the essential oil therapy of the present invention is prepared by the following materials in part by weight: 2 to 5 parts of natural essential oil, 5 to 10 parts of isopropyl myristate, 25 to 45 parts of caprylic/capric polyglycol glyceride, 8 to 15 parts of polyglyceryl fatty acid ester and 45 to 60 parts of triply distilled water. The natural essential oil nanoemulsion used for the cosmetology and the essential oil therapy of the present invention has the advantages of stable quality, difficult volatilization, extended preservation time, lasting fragrance, and lasting essential oil effect. Besides, the preparation method has the advantages of material and energy conservation, hardly any environment pollution, simple preparation process, easy practical operation, no requirement on special instrument or equipment, efficient and quick preparation, and easy industrialization.

Description

A kind ofly be used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy and preparation method thereof

Technical field

The invention belongs to medical technical field, particularly the nano-emulsion technology of preparing is applied to improve looks and the preparation of SPA natural essential oil nano-emulsion product.

Background technology

Along with the rise of natural plant beauty upsurge, have historical quintessence oil in several thousand, rise to the center of stage once again.Different is that it no longer occurs with original style and features, also no longer wears mysterious veil.Nowadays, rely on modern high-tech refinement technique, add the effect of quintessence oil uniqueness, make quintessence oil become the current cosmetics and skincare product leading role of fashion the most.

At occurring in nature, all plants all can carry out photosynthesis, and its cell can secrete the molecule of fragrant, and these molecules then can be gathered into sachet, are dispersed on petal, leaf or the trunk.After sachet refined extraction, promptly become we alleged " plants essential oil ".Quintessence oil can be by molecule be combined intos different more than 250 kinds.Therefore under the arrangement of the Nature, these molecules exist jointly with perfect ratio, make every kind of plant that its particularity all be arranged, and also quintessence oil is incomparable broadness to the profoundness effect of human body.

Quintessence oil is flower, leaf, stem, root or the fruit from plant, refines the volatile flavor of extraction by steam distillation, squeezing and pressing method, cold-maceration or solvent extraction method.Be made up of some very little molecules, they are very easy to be dissolved in ethanol/emulsifying agent, and especially fatty, this makes them very easily permeate in skin, and through entering in the body with mixing of fatty fiber.When these high flow material volatilizations, they are also absorbed by ten hundreds of cells simultaneously, by entering health in the nose breathing road, message is directly delivered to brain, running against the cerebellum system, quintessence oil controls the emotion and also controls other major functions of health, so in aromatotherapy, can be strengthened the function of physiology and psychology.The chemical constitution that all has each plants essential oil decides the mode of its fragrance, color, flowability and it and System Operation, also makes each plants essential oil that the special function speciality of one cover is respectively arranged.

Can be divided into folk prescription quintessence oil and compound essential Oil according to source of quintessence oil and uses thereof, specifically do following introduction.

(1) plants essential oil of a certain plant or plant part extraction is the folk prescription quintessence oil, with this botanical name or plant parts name nominating, generally has this comparatively strong plant scent usually, and has specific effect and characteristics of personality.We can be divided into several families with the folk prescription quintessence oil according to the classification of plant: 1. citrus: Fructus Citri Sarcodactylis, grapefruit, Fructus Citri Limoniae, Fructus Citri tangerinae or the like; 2. fragrance of a flower class: Flos Pelargonii, Anthemis nobilis, Flos Rosae Rugosae, Garden lavender, fragrant cananga, orange blossom; 3. draft class: Garden lavender, lavender, Herba Rosmarini Officinalis, Origanum majorana L., Salvia japonica Thunb.; 4. Camphora class: eucalyptus globolus, Cortex Melaleucae leucadendrae, Herba Rosmarini Officinalis, lavender, Camellia sinensis; 5. hot fragrant class: black pepper, Rhizoma Zingiberis Recens, Elettaria cardamomum (L.) Maton; 6. resinae: Olibanum, Myrrha, elemi, galbanum; 7. wooden class: cedar, Lignum Santali Albi, Masson Pine, Juniperus rigida Sieb.et Zucc., cypress; 8. soil property class: Herba Pogostemonis, Vetiveria zizanoides.

Below the effect of folk prescription quintessence oil commonly used is done simple introduction.1. Herba Rosmarini Officinalis (be fit to oiliness, Combination skin matter): prevention wrinkle, cleaning skin, the removal dandruff, moist hair, inspire enthusiasm, allaying tiredness, focus one's attention on; 2. narra (be fit to Combination, sensitive skin): the prevention wrinkle, purify skin, preserve moisture moist, promote that cell reproduces, eliminates black eye; 3. Herba Menthae (be fit to oiliness skin matter): purify skin, Bergamot Mint Extract, toxin expelling, elimination blackhead, shrink blood capillary, inspire enthusiasm, pacify emotion, allaying tiredness, focus one's attention on; 4. Flos Rosae Rugosae (being fit to dryness, hydropenia or sensitive skin): the desalination microgroove, preserve moisture, promote cell regeneration, U.S. breast, eliminate black eye, striae gravidarum and cicatrix, skin whitening, promote the sexual maturity, anti-melancholy, alleviate pressure, pleasure; 5. jasmine (be fit to sensitivity, dry skin): preserve moisture, improve sensitivity, eliminate striae gravidarum and cicatrix, promote the sexual maturity, the promotion vigor, alleviate pressure; 6. Cortex Cinnamomi (be fit to oiliness skin): prevention wrinkle, treatment comedo, fat-reducing, anti-melancholy, increase libido, pacify dejected mood; 7. Flos Caryophylli (be fit to general skin): strengthen blood circulation, infection, the coarse skin of conditioning, strengthen will power, inspire enthusiasm; 8. lavandula angustifolia (be fit to any skin): promote cell regeneration, purify skin, improve the pore obstruction, Bergamot Mint Extract, whiten, improve cicatrix, sunburn, redness, moist hair, pacify dejected, anti-melancholy, control insomnia, allaying tiredness, the pressure of releiving; 9. Myrrha (be fit to responsive, dryness skin matter): active cell,, decortication aging to drying, coarse skin have clear improvement, drive away dejected, decompress(ion).

(2) compound essential Oil is to be formed according to its different characteristics character allotment by the various plants quintessence oil, and she is mainly used in the improvement to aspects such as the potential focuses of human body.To with the control of suitable folk prescription quintessence oil and base oil and dosage, do certain compound recipe collocation and just can get a desired effect according to the concrete symptom of human body.Compound essential Oil refers to match, can make finished product after the combination allotment for the prescription quintessence oil that uses immediately, and is comparatively convenient in the use, just as preparing the patent medicine of prescription.Base oil is exactly a vegetable oil that is used for diluting the folk prescription quintessence oil, because the zest of pure quintessence oil is very strong, directly wipes on skin, can damage, so quintessence oil must dilute earlier before skin uses.Base oil be with the seed of each kind of plant, fruit via squeezing after, the non-volatile oils and fats of extraction for the first time, can be used as skin care with oil, also be the base oil of making massage oil.Itself just has curative effect the plant base oil, and vegetable oil is the good source of nutrition and energy, and health has had it just can produce heat, and it is proteinic excellent source.We can utilize the plant base oil to dilute quintessence oil.

(3) usage of quintessence oil have the method that smokes of steaming, inhalation, massotherapy, champignon gargle method, quintessence oil cutaneous scraping therapy, by deposited method, spraying, champignon shower method, or the like.

1. steam the method that smokes.Censer incense method: clear water is poured in the water container of censer, added 5-6 and drip quintessence oil.The burning-point wax candle is placed in the censer, treats that heating power makes that essential oils discharge slowly in the water.Allocate different quintessence oils and splash in the censer, just can obtain different effects, help to make different atmosphere.

Humidifier incense method: in the water tank of humidifier, directly add 5-8 and drip essential oils, quintessence oil is dispersed in the air with the water smoke of humidifier.

Warming machine scent method: cotton balls is stained with essential oils, is placed on the place that steam heating pipe distributes steam, quintessence oil is dispersed in the air with heating installation.

2. inhalation.The house inhalation: the hot water injection glass that closely boils or the washbowl of porcelain, select 1-3 kind quintessence oil to drip in hot water, sum is no more than six, after quintessence oil is fully stirred evenly.With big bath towel whole head and washbowl are covered, alternately breathe, kept 5-10 minute with mouth, nose.2 of 2+Herba Menthaes of lavandula angustifolia can be treated flu.Simple inhalation: in face-cloth or handkerchief, smell suction with quintessence oil 1-3.

3. massotherapy.Therapeutic massage is generally done from the back, and two handss are placed on the vertebrae both sides of buttocks top, and palm moves along vertebra two side direction shoulders down, during to cervical region, both hands are outside, and one side is massaged two sides of body, and one side is massaged shoulder, return starting point, must accomplish without any letup during massage, be sure not to stop midway.The 2-3 kind, the folk prescription quintessence oil that adds up to 3 is diluted in the plant massage oil of 3-4 milliliter, does face, head, neck and shoulder part or health massage.Massotherapy can be strengthened blood circulation, gets rid of vivotoxin, and different quintessence oils has different separately curative effects, and suggestion goes specialized beauty parlors to use under the professional person instructs.

4. the champignon method of gargling.2-3 is dripped quintessence oil splash in one glass of water and stir evenly, spue then 10 seconds of larynx of coughing, be repeated to whole cup water and use up, every day, champignon was gargled, and can keep breath freshening, took care of one's teeth, and reduced laryngitis.Quintessence oil commonly used: Camellia sinensis, lavandula angustifolia, Herba Menthae.During toothache,, need not dilute one of Cortex Cinnamomi, directly with cotton swab point the toothache position, can alleviate toothache.

5. quintessence oil cutaneous scraping therapy.The quintessence oil scrape therapy promptly uses plants essential oil and end oil (also available compound treatment essential oils) to be applied in by affected part or the acupoint, and the reuse acutegastroenteritis treatment device scrapes.Suggestion consulting profession aromatic fumigation therapy teacher.

6. by the method for applying.Cold compress or hot compress: 3-6 is dripped volatile oil add in cold water (cold compress) or the hot water (hot compress), after evenly stirring, immerse a towel, again towel is wrung out, apply on the whole, and with the hands applying light is covered towel at face, and the moisture that has essential oils can be infiltrated in the skin as far as possible, repeats above step 5-10 time.Body part is pressed when applying, and the ratio of water and quintessence oil is about 200ml cold water or hot water is converted 5 quintessence oils, and face then only gets final product with 1 quintessence oil.Cold compress can be alleviated, calms, be pacified.Alleviate pain disease, hot compress helps blood circulation promoting, mediate toxin or increase the infiltration of skin, quintessence oil commonly used have lavandula angustifolia, violet, Herba Rosmarini Officinalis, Flos Pelargonii, jasmine, Flos Rosae Rugosae, Fructus Citri Limoniae etc.

7. spraying.Quintessence oil is added in the distilled water, is put in the spray bottle, spray at any time in bed, on the clothes, on the furniture, on the body of house pet, on the bookcase, on the carpet, play disinfecting, deodorizing, the effect of bettering people's living environment.Quintessence oil commonly used have Herba Rosmarini Officinalis, Fructus Citri Limoniae, Fructus Citri sinensis, Herba Menthae, Flos Pelargonii, eucalyptus globolus etc., ratio is that 10 oil drippings are converted 10 milli water.

8. champignon shower method.Champignon steam bath: can select for use quintessence oils such as lavandula angustifolia, Flos Matricariae chamomillae, Herba Menthae, polyphenol powde, Fructus Citri sinensis, eucalyptus globolus, Fructus Citri Limoniae to sneak in the water, ratio is that per 600 ml waters add 2 essential oils, mixed water is watered on the thermal source of steam room (as burning red stone), steam with champignon just sheds slowly, suck these champignon steams heartily, they are the excellent maintaining agent and the detoxicant of health and skin, to antibacterial and the excellent extinction effects of viral tool.The champignon demibain: with a porcelain basin or a rustless steel basin that is merely able to hold buttocks, contain and carry half basin warm water, splash into 1-2 and drip quintessence oil, (can select lavandula angustifolia, eucalyptus globolus, Herba Rosmarini Officinalis, Herba Menthae etc.) stirred quintessence oil and opened, and carries out demibain.Champignon warm water dipping bath: put whole cylinder warm water, temperature can be accepted as good with skin, add 8-10 and drip essential oils, it is better to be sprinkled into effects such as some roseleafs in the bathtub, stir quintessence oil and petal gently it is scattered, then you just can enjoy the aromatising essence that is filling the air in the benefit one respiratory nasal air that champignon SPA brings to you, and skin pore opens, allow its aromatising essence infiltrate the skin depths, more can go deep into endothelial tissue than any organism.Behind the champignon dipping bath, will stay the very thin champignon of one deck on skin, sensation as silk.Foot bath maniluvium: prepare a basin warm water, splash into 5-6 and drip quintessence oil, more whole sole or both hands are immersed in the basin about 10 minutes, can treat muscular soreness, blood circulation promoting, but add Flos Rosae Rugosae quintessence oil skin moisturizing more the time during hand care, autumn and winter season result of use better.

But quintessence oil essence anti-communicable disease, to antibacterium, virus, mycete, can prevent inflammation, anti-spasm, promote cell metabolism and cell regeneration function, make life finer.And some quintessence oils can endocrine regulation organ, promotes hormone secretion, allows the physiology and the mental activity of human body, obtains good development.The quintessence oil known effect of behaving thisly be partial to psychological effect nothing more than releiving and inspiring enthusiasm, but the effect of quintessence oil is not only in this.Different types of quintessence oil also has various effect, for some diseases, the function of releiving with mitigation symptoms is arranged also.Quintessence oil is all helpful to many diseases, cooperates the treatment of medicine, can allow the faster of disease recovery.And use in daily life, can play purify air, sterilization, germ-resistant effect, can prevent some infectious diseases simultaneously.Quintessence oil is to following treatment of diseases effect: 1. nervous system and mental sickness; 2. eye, ear, nose, oral cavity, odontopathy; 3. the disease of respiratory system aspect; 4. the 5. disease of digestive system aspect of the disease of blood circulation aspect; 6. the disease of endocrine, metabolism, urinary system aspect; 7. the disease of sexually transmitted disease (STD), immune system aspect; 8. gynaecopathia; 9. muscle, skeletal diseases; 10. dermatosis.

Present studies show that, quintessence oil also has how new effect constantly being excavated out.For example Gal é nic laboratory finds that the Argan oil that extracts has antidotal magical effect from the berry of arganier tree; Dec1 é or laboratory has then been found a kind of beauty treatment special effect of the quintessence oil that is mixed by quintessence oils such as Semen coryli heterophyllae, wheat malt, damesca Flos Rosae Rugosae, Flos Chrysanthemi and bourbon's Flos Pelargoniis.The purpose that beauticians study quintessence oil is to allow this natural holy product help beauty treatment more, and effectively prevents and resist UV sunlight.The effect of diversified essential oil product has his own strong points, and we should each takes what he needs according to practical situation.And the penetration of quintessence oil is rapid and powerful, therefore makes sure to keep in mind and can not excessively use, and each only several of need are just much of that, in case excessive use can only be run counter to desire.The quintessence oil not diluted generally had better not directly be used.The volatility of quintessence oil is very strong, in case ingress of air will very fast volatilization, also based on this reason, quintessence oil must store with the bottle that can seal, in case uncork is used, also will cover back lid as early as possible.

Chang Yong essential oil product in the market most ofly exists with liquid form.Except Camellia sinensis and these two kinds of folk prescription quintessence oils of lavandula angustifolia can partially on a small quantity be directly used in skin, all folk prescription quintessence oils could directly use after all must being in harmonious proportion with the base oil dilution, this is exactly compound essential Oil with the product after the base oil mediation, can only dilute a kind of folk prescription quintessence oil during use, also multiple folk prescription quintessence oil can be mixed to be poured in the base oil together and dilute, the principle of dilution is: in the base oil of every 10ml, the total amount of various folk prescription quintessence oils is not above 5; Also having some products to exist with the form of mastic, as, The Body Shop (The Body Shop) the tea tree ethereal oil clean pox scrubbing cream that deoils, is a kind of clean skin product of oil-in-water type that contain even fine particle.Because the particularity of quintessence oil, so must be noted that following item in the process of using: (1) except special oral quintessence oil, other folk prescription quintessence oil or volatile oil had better not be oral, unless obtain aroma-therapeutic teacher or doctor's indication; (2) preferably avoid using quintessence oil to massage or bath in the conceived initial stage some months, because some essential oil may cause menstrual onset; (3) citrus quintessence oil (Fructus Citri Sarcodactylis phase, Fructus Citri Limoniae) can cause skin to UV sunlight allergy.Therefore, use please don't be tanned by the sun skin under sunlight in eight hours later; (4) patient who suffers from hypertension, paralysed disease, nerve and kidney aspect disease please carefully uses.Some essential oil such as cypress, Herba Rosmarini Officinalis are preferably consulted doctor or aroma-therapeutic teacher earlier before the use; (5) quintessence oil can not replace medicine.Therefore, use the back not improve, must see a doctor as symptom.Never can abandon original medicine that has used because of using quintessence oil; (6) please use by the suggestion amount.Use excessive meeting to cause minus effect, even health is caused excessive burden.Especially fragrant cananga, the excessive meeting of Salvia japonica Thunb. cause sleepiness, after drinking or should avoid using when driving; (7) could use after quintessence oil must dilute, unless other special suggestion is arranged; (8) please avoid child directly to touch, cause danger in order to avoid misapply; (9) it is intact and in the dark vial that quintessence oil must be stored in sealing, and be positioned over shady and cool place and avoid the sunlight direct projection.To prolong the essential oil life-span and to guarantee the curative effect of essential oil; (10) avoid using quintessence oil in plastics, the easy container on dissolving or greasepaint surface, when dilution essential oil, please use glass, rustless steel or ware; (11) neonate (in 2 week) can not use essential oil.One of available Garden lavender is in bathtub after 2 week.All quintessence oils of child below 12 years old must be diluted as 1/4 (adult 1, and child 1/4) of adult's use amount.Then be 1/2 of adult's consumption more than 12 years old; (12) skin or body constitution sensitivity person please carry out the sensitivity test before use earlier.

Yet the use of quintessence oil is different from ordinary cosmetics, and when magical effectiveness was arranged, it was also the same with medicine toxic, and arbitrarily for it, quintessence oil will become fragrant poison if understand the effectiveness of quintessence oil because of carelessness or not during use.At present, the product of relevant on the market quintessence oil can be divided into pure quintessence oil, contain the above compound essential Oil of two kinds of quintessence oils, contain the essential oils of certain proportion concentration quintessence oil, and a series of Related products that contain plants essential oil released of each famous brand.The beautician reminds, and uses pure quintessence oil to need certain knowledge and skill, preferably seeks help from the professional that there is qualification the beauty parlor; With respect to great majority quintessence oil is understood few personage that likes to be beautiful, the simplest method with the quintessence oil skin protection just is to use deployed ready-made product, and neither trouble has safety guarantee again.Use at present the modal mistake of quintessence oil, danger to go out on the consumption of quintessence oil, about the quintessence oil beauty treatment, preferably would rather deficiency can not be excessive, otherwise will cause the series reaction of skin.The use of quintessence oil is a relative complex, has photosensitive characteristic such as some quintessence oil, can not use daytime, otherwise skin can produce black speck and the situation of burning on the contrary after seeing light.So, under condition of unknown, the quintessence oil of had better not taking a risk to use carelessly.What also should be noted that a bit is that quintessence oil infiltrates through also needs the time metabolism in the body, except each control consumption, can not frequently use, otherwise bring the metabolism burden can for other internal organs of health.

A kind of outstanding natural essential oil that is used for beautifying skin and the permanent lasting of SPA beauty treatment and SPA effect and permanent aroma retaining effect not only will be arranged, and product also must have good stable.Yet, the volatility of quintessence oil makes product be easy to not reach permanent beauty treatment and the effect of SPA, and just do not reach the effect of permanent lasting with common preparation method essential oil product, for example: lavandula angustifolia, Flos Pelargonii, Juniperus rigida Sieb.et Zucc. and horse Holland etc. can vapor away most of composition in 20～60 minutes at all.Therefore, for fear of the destruction of ultraviolet, preserve quintessence oil with the dark glass container on the market to quintessence oil.Glass material then can be avoided the erosion of quintessence oil to the non-glass material simultaneously, so that influences the quintessence oil quality.But this still can not fundamentally solve the volatile problem of natural essential oil.

Use insufficient problem in order to solve quintessence oil, we have used pharmaceutical carrier nano-emulsion (nanoemulsion) on a kind of pharmaceutics, by being used for quintessence oil beauty treatment and SPA after the improvement, functional component is wrapped in the nano-emulsion carrier, therefore can reach slow release effect, make quintessence oil reach the effect of permanent lasting in beauty treatment and SPA application, this is inaccessible in common quintessence oil beauty treatment and SPA.Nano-emulsion is begun one's study from the forties in 20th century by Schulman, just has medicinal nano breast to come out up to late 1990s, and nano-emulsion is just really used also thing in recent years in pharmaceutical formulation.Nano-emulsion is celebrated with atomic grain, the emulsion droplet particle diameter that it is made up of oil phase, water, emulsifying agent and co-emulsifier is about the transparent or semitransparent liquid between the 10-100nm, its emulsion droplet is dispersed in the another kind of liquid and forms dispersion system of colloid, has certain opalescence, it is spherical that its emulsion droplet mostly is greatly, and size is more even, good fluidity, can not make it layering through heating or autoclaving or centrifugalize yet, belong to the thermodynamically metastable fixed system usually.Two fundamental differences of nano-emulsion and common emulsion are: at first, the formation of ordinary emulsion generally needs the external world that energy is provided, and the formation of nano-emulsion is spontaneous; Secondly, ordinary emulsion is a thermodynamic unstable system, in depositing process, will take place coalescent and finally be divided into oil, water is biphase.And nano-emulsion is a thermodynamic stable system, can not take place coalescent.

The nano-emulsion carrier is applied among quintessence oil beauty treatment and the SPA, and this does not still have precedent at home and abroad, and many application technologies still belong to blank.Therefore at present in the actual application of nano-emulsion, also there are a lot of uncertain problems: how to select lower emulsifying agent of toxicity and co-emulsifier and natural essential oil coupling, thereby reduce the toxicity and the skin irritation of natural essential oil nano-emulsion; How to reduce the consumption of emulsifying agent and co-emulsifier; How to determine the emulsifying agent and the co-emulsifier of optimum matching, improve the stability and the anti-heliosensitivity of natural essential oil nano-emulsion; How to determine the prescription and the process for producing of best natural essential oil nano-emulsion; How to select and kind and the application dose or the concentration of definite essence oil nanometer Ruzhong functional component, or the like a unsolved difficult problem, all will be resolved in the present invention.

Summary of the invention

1. in order to overcome the deficiency that exists in the background technology: the volatility of (1) quintessence oil is very strong, in case ingress of air will very fast volatilization, must store poor stability with the bottle that can seal.(2) penetration of quintessence oil is rapid and powerful, can not excessively use, in case excessive use can only be run counter to desire, folk prescription quintessence oil individual preparation is used inconvenient.(2) highly volatile of quintessence oil makes product not reach the effect of permanent lasting on beauty treatment and s skin S PA, does not promptly reach the persistent efficacy effect of quintessence oil.

The present invention is directed to the shortcoming and the problem that exist in the background technology for this reason and improved, innovate, provide a kind of and be used to improve looks and the permanent fragrance-lasting natural essence oil nanometer emulsion formulation of SPA and preparation method thereof.

2. technical scheme of the present invention

(1) natural essential oil nano-emulsion of the present invention is to be made by the following weight parts proportion raw material: natural essential oil 2-5, isopropyl myristate 5-10, sad capric acid polyethyleneglycol glyceride 25-45, polyglyceryl fatty acid ester 8-15, triple distillation water 45-60;

(2) natural essential oil (is example with the tea tree ethereal oil) the preferred weight proportion of each raw material of nano-emulsion is: tea tree ethereal oil 3, isopropyl myristate 7, sad capric acid polyethyleneglycol glyceride 30, polyglyceryl fatty acid ester 10, triple distillation water 50;

(3) principles of formulating prescriptions of the present invention: plants essential oil mainly acts on human body by following approach: respiratory system-plants essential oil molecule stimulates olfactory nerves by breath, olfactory nerves reaches big mesencephalic centre with stimulation, brain produces excited, the one side dominating neural, play and regulate neururgic function, advance alveolar by respiratory system on the other hand, enter blood by blood circulation and be delivered directly to each position of whole body.Nervous system-directly enter subcutaneous by affinity interaction, the plants essential oil molecule excites nerve on the one hand, final neural activity and the internal recycle regulated, steady statues such as interior environment have directly been changed on the other hand, make the movable quickening of body fluid, thereby environment in improving further reaches the effect of regulating whole body and mind; Metabolic system-change the living environment of local organization, cell rapidly by affinity interaction is accelerated its metabolism, solves some problems that cause because of local dysbolismus comprehensively; Promote exchange: advance subcutaneous tissue by affinity interaction, enter blood and lymph through the body fluid exchange again, promoted blood and lymph circulation, accelerated the metabolism of human body; Directly direct killing pathogenic bacteria of effect-quintessence oil molecule and microorganism, enter the immunity of human body quintessence oil molecular energy enhancing human body, and then nervous system regulation, hormonal system, digestion and Excretory system, and enhance immunity ability, promote cell regeneration, thereby reach health preserving body-building, tranquilization and refreshment, the antibiotic effect such as smelly, cosmetic slimming that presses down.But, the volatility of quintessence oil is very strong, in case the very fast volatilization of ingress of air, therefore, after being used for skin, can in air, run off greatly, and its fragrance can not be lasting, and be prepared into nano-emulsion preparation, because oil phase is wrapped in aqueous phase, will reduce the aerial volatilization of quintessence oil greatly, is convenient to the preservation of long period; And, be used for skin after, can effectively promote the absorption of quintessence oil.Such as, tea tree ethereal oil has potent germ-resistant effect, BMJ report " this kind quintessence oil is except that surprising sterilizing power; can not produce any infringement to organizing; this point is not seen before, but because general antibacterial except killing bacteria, also can destroy normal structure ".Studies show that, only need the concentration of tea tree ethereal oil 0.25% just can suppress 90% escherichia coli, staphylococcus aureus also only needs 0.5%, treat agent than the antiviral that the chemicals of general degerming is strong 13 times, can be listed as anti-virus, antibacterial and the fungus that causes inflammation effectively, the injury of microorganism can also be resisted, the immunity of health can certainly be encouraged; Also eliminate the phlegm, prevent effects such as carsickness, anti-acne, antifungal in addition.Herba Lysimachiae foenum-graeci quintessence oil is the most widely used quintessence oil, also is that minority can directly spread upon one of quintessence oil on the skin, and it can rebuild poised state, no matter is psychology or health, allows people's health be in harmonious poised state energy spontaneous recovery.Its main feature is as follows: therapeutical effect, stable emotion, eliminate and dejectedly, flat comfort emotional trauma, remove worry, the muscle fatigue of releiving, promote cell regeneration, accelerating wound healing, is nursed one's health skin care at convergence oiliness and skin sore skin.

(4) the present invention is used to improve looks and the preparation method of permanent fragrance-lasting natural quintessence oil (is example with the tea tree ethereal oil) nano-emulsion of SPA is:

1. get tri-distilled water and place the container of washing, clearly be labeled as water (I) with colored marking pen.

2. accurately take by weighing isopropyl myristate with analytical balance, place another, add tea tree ethereal oil through among container of washing, ultrasonic mixing, and with the clear oil phase (II) that is labeled as of marking pen.

3. in sad capric acid polyethyleneglycol glyceride (S): the ratio of polyglyceryl fatty acid ester (C)=3: 1 accurately takes by weighing sad capric acid polyethyleneglycol glyceride and polyglyceryl fatty acid ester respectively, and both is placed among the 3rd container that process was washed; Abundant mix homogeneously, and with the clear S/C mixture (III) that is labeled as of marking pen.

4. according to oil phase (II): S/C (III): the ratio of water (I)=10%: 40%: 50%, the phase (I) of fetching water respectively earlier, S/C mixture (IH) place among the 4th container that process was washed.After two liquid phases are fully mixed, under 25 ℃ of conditions of room temperature or in the natural room temperature, start the time constant-temperature magnetic stirring apparatus, with 200rpmmin ^-1Rotating speed magnetic agitation 5min, then, in this container, directly add oil phase (II) again, and with whole system in ultrasonator about ultrasonic 10min, perhaps under 25 ℃ of conditions of room temperature or in the natural room temperature, start the time constant-temperature magnetic stirring apparatus, with 200rpmmin ^-1Rotating speed magnetic agitation 20min.

5. observe its outward appearance colourless in this way, limpid transparent, fluidity and good dispersion, obviously visible opalescence person is arranged, be the permanent lasting tea tree ethereal oil nano-emulsion of beautifying skin and SPA.

6. this essence oil nanometer breast is sub-packed in rapidly among the lucifuge glass container of different size, adds a cover, sterilization, packing, and place to preserve among the dry shady and cool room temperature of ventilating and get final product.

(5) usage and dosage: the time spent, the permanent lasting essence oil nanometer breast emulsion of beauty treatment of bark fetching skin and SPA is applied to beauty treatment or SPA position in right amount.

(6) storage: airtight, put shady and cool dry place and preserve.

3. beneficial effect of the present invention

Outstanding innovative point of the present invention is that the natural essential oil functional component is successfully combined application with the nano-emulsion technology, is formed for improving looks and the permanent fragrance-lasting natural essence oil nanometer breast of SPA, and this is unprecedented.And the present invention is used to improve looks and has improved the stability of quintessence oil with the permanent fragrance-lasting natural essence oil nanometer breast of SPA, the energy long preservation, and can in skin, slowly discharge, reached the quintessence oil long-acting, solved the difficult problem of the long-acting application of quintessence oil.The preparation composition of this product is simplified in addition, and effect improves, and the nano-emulsion particle diameter trickle, be evenly distributed, be easy to Transdermal absorption more, with low cost, preparation technology is simple, and practical operation is easy, preparation is fast efficient, does not need special instruments and equipment, is easy to extensive industrialization.Specifically, the beneficial effect that being used to of adopting that the technology of the present invention prepares improves looks and the permanent fragrance-lasting natural essence oil nanometer breast of SPA is embodied mainly shows as:

3.1 improve the stability of quintessence oil functional component and system.The volatility of natural essential oil and heliosensitivity have reduced the stability of product, and great limitation is in use arranged, and combine the stability that should be used for improving product with the nano-emulsion technology.Come the stability of test products by following detection., undertaken the requirement that the influence factor of preparation stability tests defined with reference to China by following three aspects.

3.1.1 strong illumination test: place under the illumination of 2000～40001x, measured in the 5th day, the tenth day, the result does not relatively have difference before placing.

3.1.2 hot test: place respectively in 40 ℃, 60 ℃, 80 ℃ in airtight vessel, measured in the 5th day, the tenth day, the result does not relatively have notable difference before placing; High humility test is placed under 25 ℃, relative humidity 75% and 92.5% condition in airtight vessel, measures in the 5th day, the tenth day, and the result is with no significant difference relatively before placing, but with market on common quintessence oil compared evident difference.Keep sample to investigate to test by accelerated test and room temperature in addition and all prove having good stability of natural essential oil effect nanometer emulsion.

3.1.3 accelerated test: place with commercially available being packaged under 30 ± 2 ℃/RH (60 ± 5 ℃) the % condition, in the time of 0,1,2,3,6 month, measure study on the stability project (having or not the content of layering, medicine and related substance etc.).

The investigation 3.1.4 room temperature keeps sample: with commercially available back (25 ℃ ± 2 ℃/RH75%) carry out under the condition of room temperature, press certain hour (0,1,3,6,12,24,36 month) at interval, measure study on the stability project (having or not the content of layering, medicine and related substance etc.).

3.1.5 high speed centrifugation separation test: get natural essential oil nano-emulsion product and traditional natural essential oil product respectively, and place respectively high speed centrifuge supporting centrifuge tube, with 13000rpmmin ^-1The centrifugal 30min of speed, through measuring index of correlation, find that the natural essential oil nano-emulsion product that adopts the technology of the present invention to prepare is higher than the natural essential oil product stability of traditional handicraft preparation.

3.2 make functional component reach the effect of permanent lasting.The natural essential oil nanotechnology is a scientific achievement up-to-date, that gold content is high, collection beauty treatment, SPA, lasting and one.And natural essential oil is become the nano-emulsion configuration state by the nano-emulsion vehicle treated, is very easily absorbed by skin, urgees to ooze under the function at the nano-emulsion carrier, evenly is deep into skin inside, slowly discharges quintessence oil.Through facts have proved that product has strong infiltration, easily absorb, not volatile, etc. advantage, and natural essential oil fragrance retention time reaches wheat harvesting period.

3.3 the sense organ and the comfort of product improve greatly.Nanoemulsions as two kinds of immiscible liquid according to a certain percentage, naturally the nano-emulsion product cut size that forms under the combined effect of surfactant and cosurfactant is all at nanoscale, and the overwhelming majority is about 43.8 nanometers, centralized particle diameter, uniformity are better, thermodynamically stable, isotropic, good fluidity, as clear as crystal, glittering and translucent, slightly opalescent dispersion, have good perception, and actual comfort is splendid.Quintessence oil has reduced the volatility of quintessence oil in nanometer cream nuclear in addition, makes quintessence oil slowly be discharged into external environment condition from quintessence oil, reaches the effect of permanent lasting, and this is that common essential oil product is incomparable.

3.4 improve the dissolubility of functional component in carrier.The heavy dose of solubilising of natural essential oil functional component in the nano-emulsion system, make the concentration of natural essential oil functional component in the fine droplet of nano-emulsion can reach very high, the interfacial film that this forms by surfactant molecule fast for the natural essential oil functional component, thereby, when it is used on skin of face, form natural essential oil functional component bank rapidly, and make it according to the gradient penetration rule, form from high to low, the passive target effect and the sustained-release and controlled release effect of ecto-entad, reach efficient release and significant curative effect.The increase greatly of concentration, the amount that makes the quintessence oil of equal effect need also reduces.

3.5 security of products improves greatly.Adopt nano-emulsion technology of preparing of the present invention, natural essential oil functional component addition is reduced greatly, and access times also obviously reduce, and have solved the easy skin permeation of micromolecule quintessence oil, the problem that can not slowly steadily act on, the safety that quintessence oil is used by the nano-emulsion technology is improved.

In sum, natural essential oil has reduced the volatility of quintessence oil in nanometer cream nuclear, make quintessence oil slowly be discharged into external environment condition from quintessence oil, reaches the effect of permanent lasting, promptly reaches persistent efficacy effect, and this is that common essential oil product is incomparable.Under the identical or better situation of result of use, reduced the application concentration of natural essential oil simultaneously, this has solved high concentration quintessence oil loss, some toxic and side effects to skin on the one hand, for the also raising greatly of safety in utilization of natural essential oil; On the other hand, natural essential oil has reduced the use amount (under the equal effect condition) of natural essential oil, has reduced resource consumption and waste, and product cost is reduced greatly.And the present invention is used to improve looks and the preparation composition of the permanent fragrance-lasting natural essence oil nanometer breast of SPA is simplified, preparation technology is simple, practical operation is easy, the preparation is efficiently quick, with low cost, do not need special instruments and equipment, the suitability for industrialized production scale is changeable, do not need the external world that energy is provided, do not need heating, reduced the use of the energy and the pollution of environment.Therefore industrialization technology level of the present invention, product quality, safety, effect etc. all obviously improve and improve.

4. the present invention has following effect and function:

4.1 improve the stability of natural essential oil functional component in product.The volatility of quintessence oil is very strong, in case ingress of air will very fast volatilization, also based on this reason, quintessence oil must store with the bottle that can seal, in case uncork is used, also will cover back lid as early as possible.This makes the stability of product lower, and has limited to the use of natural essential oil.And use nano-emulsion technology preparation of the present invention, the effect nanometer emulsion of its formation then can improve its stability significantly, reduces the volatilization of natural essential oil greatly.

4.2 improve the comfortableness of natural essential oil product.Nanoemulsions as two kinds of immiscible liquid according to a certain percentage, under the combined effect of surfactant and cosurfactant, form thermodynamically stable, isotropic, good fluidity, as clear as crystal, glittering and translucent, slightly opalescent dispersion naturally, have good perception official property, and actual comfort is splendid.

4.3 help the adjustment of natural essential oil formula for a product.The composition of nano-emulsion carrier is simple and clear, comprises that mainly four of oil phase, water, surfactant and cosurfactants are mutually.By this four phase liquid and hydrophile-lipophile balance value difference, can obtain oil-in-water type nanoemulsions (O/W), water-in-oil type nanoemulsion liquid (W/O) and doubly-linked ideotype nanoemulsions as required.Adjust and change conveniently because of its dosage form, therefore can prepare different natural essential oil nano-emulsions in actual applications according to the physicochemical property of season, skin type and functional component.

4.4 improved product antiseptical ability, minimizing does not simultaneously even add anticorrosive additive.Though it is corrupt, rotten that natural essential oil itself is difficult for, but in application product, still need anticorrosion, and the nano-emulsion carrier itself has certain antibacterial action, need not add chemical preservative and just can prevent product rotten, and certain inhibitory or killing effect can be arranged the certain micro-organisms that has skin surface.

4.5 improved the dissolubility of natural essential oil in goods support, reduced the use amount of natural essential oil.The concentration of natural essential oil functional component in the fine droplet of nano-emulsion can reach very high.In addition, the natural essential oil functional component is formed interfacial film by surfactant molecule fast, thereby, when it is used on skin of face, form natural essential oil functional component bank rapidly, and make it according to the gradient penetration rule, form from high to low, the passive target effect and the sustained-release and controlled release effect of ecto-entad, reach the curative effect of efficient and slow release.

4.6 the natural essential oil nano-emulsion is prepared under temperature conditions Celsius about 25 ℃, therefore can thereby not influence the content of natural essential oil in carrier, activity because of the former of preparation temperature.This is even more important for volatile natural essential oil.

4.7 utilize the suitability for industrialized production of natural essential oil product.Nano-emulsion preparation technology is terse, technical operation is simple, saves time to save trouble; And whole process of preparation does not need specific apparatus and equipment, laborsaving saving money; And, carry out nano-emulsion preparation can be under normal temperature condition spontaneous formation nano-emulsion system, do not need electric power and other energy, but both energy savings and cost are free from environmental pollution again.

4.8 can be applied to other subject.The nano-emulsion technology can be applied to daily chemical products, such as cleaning, sterilize, add incense products.Because the product efficacy composition is processed into nanoscale size, has high density, strong infiltration, easily absorb, not volatile, anti-advantage such as wash by rubbing with the hands, very easily structure and the fibrous layer with processed product absorbs, can be deep in the fibrous layer of processed product, through facts have proved that the fragrance retention time reaches wheat harvesting period.

5. the know-why of nano-emulsion preparation of the present invention

The method for preparing at present nano-emulsion mainly is divided into high energy emulsion process and low-energy emulsification method.The high energy emulsion process prepares nano-emulsion as the term suggests be to carry out the nano-emulsion preparation by the energy that various forms produces, and usually it is divided into three kinds of methods, i.e. shear agitation method, high pressure homogenizer homogenate method and supersound method.The shear agitation method can be controlled particle diameter well, and the prescription composition can have a lot of selections.High pressure homogenizer homogenate method is most widely used in commercial production, and general high pressure homogenizer operating pressure is 50-100MPa, and new its pressure of improved high pressure homogenizer can be up to 350MPa.Supersound method is reducing aspect the particle diameter very effectively, adopt the probe Ultrasound Instrument usually, but this method only is fit to the preparation small amount of sample, and the heating of in use popping one's head in can produce iron filings and go forward side by side into medicinal liquid, so the influence of quality to medicinal liquid of should noting popping one's head in.In addition, the dispersion of nano-emulsion also is subjected to the influence of supersonic frequency and ultrasonic time, and the hydrophobicity of polymerization single polymerization monomer is strong more, and required ultrasonic time is long more.

And the low-energy emulsification legal system is equipped with nano-emulsion is the physicochemical property of utilizing its system, makes the dispersion of emulsion droplet can spontaneous generation nano-emulsion.The low-energy emulsification legal system is equipped with nano-emulsion and is divided into the phase transition temperature emulsion process usually and changes emulsion process mutually.The phase transition temperature emulsion process is used more extensive in commercial production, it is a characteristic of utilizing the dissolubility of polyoxyethylene-type non-ionic surface active agent to change along with variation of temperature, with water with oil phase is disposable is mixed in together, when temperature raises, hydrogen bond on the surfactant molecule comes off, the polyoxyethylene chain dehydration, and the molecule hydrophobicity strengthens, spontaneous curvature becomes negative value, forms aqueous reverse micelle (w/o type Emulsion); When temperature was reduced to phase transition temperature, surfactant spontaneously made curvature approach zero, and formed layer structure; When temperature further reduced, middle a part layer of surfactant produced very big forward curvature, forms trickle oiliness micelle (O/W type Emulsion), and emulsion droplet forms the process of nano-emulsion in the phase transition temperature method that Here it is.The phase transition temperature emulsion process makes full use of surfactant molecule low-down interfacial tension when phase transition temperature and promotes emulsifying, for non-ionic surface active agent, by changing the temperature of system, O/W type Emulsion when the w/o type Emulsion when impelling high temperature becomes low temperature, and in cooling procedure, system becomes minimum table and tension force has promoted the formation of fine dispersion oil droplet from zero curvature.In addition, except temperature, other parameters such as salinity and pH value also can exert an influence to emulsifying.Because the coalescent speed of emulsion droplet is very fast, so although emulsifying effectiveness is fine when phase transition temperature, but the nano-emulsion of preparing is extremely unstable, but can or be heated to 25-30 ℃ by quick cooling, obtain the Emulsion (O/W or w/o type) of dynamic stability, it can reach very little particle diameter and very narrow particle size distribution range; If heating or refrigerative speed are fast inadequately, the merging of emulsion droplet will become major trend, thereby forms polydisperse thick Emulsion.And change the emulsifying rule mutually is continuously water to be added among the oil phase, during beginning, because the oil phase surplus forms w/o type Emulsion, but along with the increase of water ratio, has constantly changed wherein surfactant curvature, and water droplet coalesces together gradually; At the Emulsion phase inversion point, surfactant forms layer structure, and this moment, the surface tension minimum helped to form very little dispersion emulsion droplet.After the Emulsion phase inversion point, along with the further increase of water, O/W type Emulsion forms, and emulsion droplet forms the process of nano-emulsion in Here it is the phase transition method.Changing in the emulsion process floating forming process of dripping mutually needs short chain surfactants, and it forms flexible monolayer at oil-water interfaces, causes the formation at the transformation temperature nano-emulsion.During this transfer, surfactant produces lowest surface tension, and this helps to form fine emulsion droplet.

The volume that the principal element that influences the nano-emulsion type has oil phase and a water is both viscosity differences and the kind of surfactant when.Wherein, surfactant is the most important to the formation and the character of nano-emulsion, its molecule generally is to be made of nonpolar, lipophilic hydrocarbon chain and polar, hydrophilic group two parts, have not only oleophylic but also hydrophilic amphipathic characteristic, this kind molecule has and can reach in the inner critical nature that forms micelle of solution by oriented adsorption on various interfaces, has the surface tension that reduces the interface, the type of decision nano-emulsion, produce interfacial tension gradient, cause static and steric hindrance repelling effect etc.In the selection of surfactant, what at first need to consider is exactly its hydrophile-lipophile balance (HLB) value, as select HLB surfactant between 8～18, then it will form O/W type nano-emulsion, select the surfactant between 3～6 then easily to form the w/o type nano-emulsion.In most systems, forming the stabilized nano breast needs the participation of cosurfactant, its effect has following characteristics: increase the interfacial film flowability: when generating nanoemulsions, the interface bends, need interfacial tension and interfacial pressure work done, and when big drop is dispersed into droplet, needing interface deformation to reform, these need the interface bending energy.Add cosurfactant, can reduce the interface rigidity, increase the interface flowability, required interface bending energy when reducing nanoemulsions and generating makes the easy spontaneous generation of nanoemulsions; The HLB value of reconciliation statement surface-active agent: when forming nanoemulsions, for making surfactant that a large amount of absorption be arranged on oil/water surface, must be with the suitable surfactant of HLB value, if the HLB value of surfactant is improper, available cosurfactant is adjusted to desired value.Reduce interfacial tension: for single surfactant, usually before reducing to zero, interfacial tension reached CMC (in solution, forming the required least concentration of micelle), add different cosurfactants, interfacial tension is further reduced, even be negative value, this moment, the interface expansion generated intact drop, cause more surfactant and cosurfactant in Interfacial Adsorption, thereby greatly reduce the concentration of surfactant and cosurfactant in the system solution, interfacial tension be again on the occasion of, generated nanoemulsions.

As oil phase, sad capric acid polyethyleneglycol glyceride is as emulsifying agent by isopropyl myristate in the present invention, and polyglyceryl fatty acid ester is a co-emulsifier, and distilled water is as water.Because the sad capric acid polyethyleneglycol glyceride of emulsifying agent helps the mutual blending of oil phase isopropyl myristate and water distilled water and forms emulsion droplet, and the co-emulsifier polyglyceryl fatty acid ester also is the essential condition that this natural essential oil nano-emulsion forms.Particularly because polyglyceryl fatty acid ester and the special separately physicochemical property of sad capric acid polyethyleneglycol glyceride, the co-emulsifier polyglyceryl fatty acid ester can be inserted in the limitans with the sad capric acid polyethyleneglycol glyceride of emulsifying agent, form complex condensed film, to improve the coagulability and the compliance of film, and can increase dissolubility with sad capric acid polyethyleneglycol glyceride, and further reduce interfacial tension, help forming stabilized nano breast more.Sad capric acid polyethyleneglycol glyceride often is subjected to the restriction of dissolubility, and polyglyceryl fatty acid ester can make the dissolubility of sad capric acid polyethyleneglycol glyceride increase, the easier further dispersion of emulsion droplet, particularly sad capric acid polyethyleneglycol glyceride and polyglyceryl fatty acid ester are in isopropyl myristate and the further a large amount of absorption of distilled water boundary, both are reduced the concentration in continuous phase, interfacial tension become again on the occasion of, make it progressively to form that stable being used to improved looks and the technology of preparing of the permanent fragrance-lasting natural essence oil nanometer breast of SPA.

Most of quintessence oil is flower, leaf, stem, root or the fruit from plant, to refine the Vegetable oil lipoprotein of extraction, molten 70 times more than than common medical herbs of the property of medicine by vapour method, squeezing and pressing method, cold-maceration or solvent extraction method extraction.People dissolve in blood of human body and the lymph fluid these quintessence oils by methods such as massage, suction, hot compress, immersion, steaming are smoked.Natural essential oil has high volatile volatile, though the volatility of different cultivars quintessence oil has speed difference.Its molecule is little, very easy being absorbed by the body, and energy rapid permeability human organ, superfluous components is excreted again, whole process only needs dozens of minutes, and the fragrance of plant itself also directly stimulates hypophysis, ferment and hormonal secretion, function in the balanced body plays the effect of beauty and skin care.But because highly volatile reason makes mill run not reach the effect of beauty treatment and the permanent lasting of SPA.

And be used to improve looks and the preparation principle of the permanent fragrance-lasting natural essence oil nanometer breast of SPA is drawn the pseudo-ternary phase diagram of natural essential oil nano-emulsion and nano-emulsion region area relative value size is carried out computational analysis by origin7.0 software.Owing to can make oil phase and water blending and form the nanometer emulsion droplet by emulsifying agent and co-emulsifier, and co-emulsifier can be inserted in the limitans of emulsifying agent, make it to form complex condensed film, improve the coagulability and the compliance of film, and can increase the dissolubility of emulsifying agent, further reduce interfacial tension, more stable after helping nano-emulsion and forming.Consider that emulsifying agent is subjected to the restriction of dissolubility, and co-emulsifier can make the dissolubility of emulsifying agent increase, the easier dispersion of emulsion droplet, particularly emulsifying agent and co-emulsifier are in oil phase and the further a large amount of absorption of aqueous phase interface, both are reduced the concentration in continuous phase, interfacial tension become again on the occasion of, make it progressively to form stabilized nano breast more.Meanwhile natural essential oil is dissolved among the oil phase isopropyl myristate, and under the effect of compositions such as S/C mixture that sad capric acid polyethyleneglycol glyceride and polyglyceryl fatty acid ester form and distilled water and participating in, permanent fragrance-lasting natural essence oil nanometer breast with SPA naturally is formed for improving looks.In natural essential oil nano-emulsion preparation prescription, isopropyl myristate is as oil phase, and sad capric acid polyethyleneglycol glyceride is as emulsifying agent, and polyglyceryl fatty acid ester is a co-emulsifier, and distilled water is as water.

Different natural essential oil functional components have different physicochemical properties, and actual concentration of adding is also variant, and therefore, the prescription of the nano-emulsion of preparation is also had nothing in common with each other.In general, the basis of prescription is oil phase, water, emulsifying agent (surfactant), co-emulsifier (cosurfactant), and the functional component of prescription then carries out prescription according to the actual needs of skin beauty health-care and treatment damage capacitive problem skin.During preparation, can it be dissolved in the corresponding phase liquid respectively earlier according to the dissolubility of natural essential oil functional component.After oil phase, emulsifying agent, co-emulsifier have been determined, can find out the nano-emulsion zone by pseudo-three-phase diagram, thereby determine their consumptions separately.Concrete operations are as follows: in oil phase, water, emulsifying agent, four components of co-emulsifier, generally emulsifying agent and consumption thereof can be fixed, three summits of oil phase, water, three ingredients constitute equilateral triangles of co-emulsifier make phasor at constant temperature.Oil phase, emulsifying agent, the co-emulsifier mixed solution of definite composition are carried out titration with water.Whether with dropper carry out titration when adding water at every turn, stir simultaneously, examining then is transparent nano-emulsion or muddy nano-emulsion.Adding after water makes nano-emulsion reach complete equipilibrium, nano-emulsion should be transparent, clarification, flow and have opalescent liquid.Certainly, also can be with emulsifying agent and co-emulsifier mixed solution a summit as equilateral triangle, then oil phase and water are formed pseudo-three-phase diagram as two other summit of equilateral triangle.

The concrete preparation method and the basic step of water-in-oil type (W/O) nano-emulsion are: determine each prescription from pseudo-three-phase diagram after, earlier with hydrophilic emulsifier and co-emulsifier mixed as requested, stir at normal temperatures, add a certain proportion of oil phase again, after continuing to stir, the water phase titration promptly gets water-in-oil type (W/O) nano-emulsion till muddy liquid becomes clear.The concrete preparation method and the basic step of oil-in-water type (O/W) nano-emulsion are: select oil phase and oleophilic emulsifier earlier, and according to certain ratio this oleophilic emulsifier is dissolved in the oil phase, constantly stir, make it to mix fully; This mixing material is slowly added aqueous phase, constantly stir; As the consumption of known co-emulsifier, then it directly can be added aqueous phase, as not know the consumption of co-emulsifier, the above-mentioned oil-water mixture body of then available this co-emulsifier titration promptly gets the oil-in-water type nano-emulsion till becoming clear.

6. Analysis and Identification of the present invention

6.1 the naked eyes outward appearance is observed: most of natural essential oil appearance colorless of the present invention's preparation or chlorine slightly, clear, visible opalescence, have certain fluidity.

6.2 the electron microscopic morphology of nano-emulsion is observed: the Sodium phosphotungstate drips of solution of 2-3% is added on the stencil plate, in addition the natural essential oil nano-emulsion is added drop-wise to the copper mesh front, go too much sample liquid with the filter paper suction, the copper mesh face down is covered dye liquor drips, negative staining 30min takes out copper mesh, draws too much dye liquor with filter paper, with copper mesh face up place glass dish to dry 2 hours naturally after, under transmission electron microscope, carry out form observation and take a picture.The common atrament of the common form of the Electronic Speculum of nano-emulsion is the nano-emulsion droplet, and nano-emulsion negative staining form whiteness then is the nano-emulsion droplet, and background is a black.

6.3 nano-emulsion size, distribution and potential measurement: adopt the Ma Erwen assay method, get nano-emulsion 2ml and inject the test cup, measure size and the distribution and the zeta current potential of nano-emulsion particle by particle size analyzer.Be used to improve looks and the mean diameter of the permanent lasting tea tree ethereal oil nano-emulsion particle of SPA is 43.8nm, the zeta current potential is 2.3mv, meets the characteristics of nano-emulsion.

6.4 the mensuration of natural essential oil in the nano-emulsion: it is an amount of that precision takes by weighing the natural essential oil nano-emulsion, puts respectively in the 25mL measuring bottle, adds compatible solvent dilution to the scale of quintessence oil and shake up, as need testing solution.The assay of natural essential oil and related substance check that assay carries out assay with reference to its quality standard and content assaying method, be used for improving looks and the permanent fragrance-lasting natural tea tree ethereal oil nano-emulsion Oleum Camelliae essential oil content of SPA reaches 2.9%, add fashionable very nearly the same with preparation.

6.5 the evaluation of nano-emulsion type: adopt staining to identify that the cardinal principle of the type of nano-emulsion is to judge the type of nano-emulsion by tonyred (oiliness dyestuff) and methylene blue (water-soluble dye) red or blue diffusion speed in nano-emulsion: water-soluble dye in the diffusion of O/W nano-emulsion soon, because O/W nano-emulsion continuous phase is water, water-soluble dye is faster than spreading at oil phase at water, as a same reason, oil-soluble dyes are fast in the diffusion of W/O nano-emulsion.Through identifying, be used to improve looks and the permanent fragrance-lasting natural tea tree ethereal oil nano-emulsion of SPA is an O/W type nano-emulsion.

7. preparation points for attention of the present invention:

7.1 container used during the product preparation must strictly according to the rules clean and sterilization, preferably adopting strong acid to soak back water dashes, clear water washes clean again, dry with distilled water immersion the back, place after the baking oven high-temperature sterilization drying standby again, but the materials such as antiseptic that preparation process is avoided the contacting metal ion and contained release formaldehyde, equipment application of pure rustless steel; Compositions such as prescription water must not be brought metal ion into, in order to avoid influence product quality and stability.

7.2 incomplete same because of the quality standard of present quintessence oil functional component of the same race and raw material, particularly there is some difference for content and purity, therefore, when carrying out the product preparation, the quality and the source of the hig diligence raw material of being sure to.After in the end determining formula material and preparation method, do not change raw material sources and quality more in principle, in order to avoid influence product quality, stability and result of use.

7.3 avoid using quintessence oil in plastics, the easy container on dissolving or greasepaint surface, when dilution essential oil, please use glass, rustless steel or ware.

7.4 the ratio of oil phase, water, emulsifying agent, co-emulsifier is very crucial, should prepare in strict accordance with above-mentioned dosage and method.The ratio of the oil phase of the present invention design, water, emulsifying agent, co-emulsifier is only decided at a certain or two kinds of functional components, during other effect nanometer emulsion of reality preparation, can suitably adjust the ratio of oil phase, water, emulsifying agent, co-emulsifier according to the concrete condition that occurs in the process for preparation, but had better not surpass 5% up and down, the ratio of adjusting 2-3% usually gets final product.When carrying out this product preparation, should add according to above-mentioned flow sequence, its additive capacity must require accurate, in order to avoid muddiness occurs, influences the preparation of whole nano-emulsion system.

7.5 when preparation Camellia sinensis essence oil nanometer breast, need select and definite S/C mass ratio (km value), and definite S/C mass ratio (km value) 1,2,3,4, be to carry out the oily biphase binary liquid test of titration water and last definite by ability after the pseudo-ternary phase diagram analysis, only, be only comparatively ideal km value determining and calculating the km value that obtains maximum nano-emulsion zone.Above-mentioned preparation method determines that by testing Km value has been 3, but actual when carrying out the product preparation, surfactant (S) and cosurfactant in this ratio still must be after mixing also will be more abundant mixing, prepare effect and quality in order to avoid influence.

7.6 because the proportion or the different relation of density of liquid solution, when carrying out the product preparation, the capacity of selected preparation container must be a bit larger tham the product volume of actual preparation, as prepare the 100g product, need to select the preparation container of 150-200mL, like this, both be convenient to each composition and fully mixed and dissolve, also be convenient to ultrasonic concussion or stirring and evenly mixing, and be unlikely to overflow outward.

7.7 though blank nano-emulsion itself has the characteristics of certain bacteria growing inhibiting, but owing in the formula for a product other interpolation antiseptic is not arranged, so when carrying out the product preparation, except that the preparation container must carry out strictness cleaning and sterilization, also should be during concrete the preparation in strict accordance with rule of operation, under aseptic condition and according to the sterile working, require to operate as far as possible, avoid product nano-emulsion product to be polluted, influence product quality.

7.8 the product that preparation is finished don't fail to lucifuge be packed, also place cool place, drying, ventilation, lucifuge place to preserve as far as possible.In addition, after product uses at ordinary times, need not place low temperature or refrigerator to preserve, but must seal tightly, be positioned over natural room temperature and get final product.

Though 7.9 nano-emulsion belongs to the thermodynamically metastable fixed system, temperature is too high and cross the low stability that then directly has influence on it, emulsifying agent mainly is distributed in water during low temperature, then mainly is distributed in oil phase during high temperature.The rising of temperature because the lipophile of emulsifying agent increases, hydrophilic weakens, forms reverse micelle, so the hydrophilic group of nonionic emulsifier weakens with the HYDROGEN BOND INTENSITY that water forms, even ruptures, and will cause emulsifying agent to be separated out from aqueous solution, promptly produces the cloud point phenomenon.Temperature reduces, because the hydrophilic of nonionic emulsifier is greater than lipophile, it and water form positive micelle, therefore, also will produce similar cloud point phenomenon.Have only the system of working as to be in certain medium temperature, nonionic emulsifier then can form the stratiform micelle, thereby really reaches balance, and system is also kept balance and stable.Therefore, the effect nanometer emulsion that requires to prepare is preserved at normal temperatures.

Description of drawings (is example with the tea tree ethereal oil)

Fig. 1. be used to improve looks and permanent lasting tea tree ethereal oil nano-emulsion preparation technology's flow process and the technology path of SPA.

The specific embodiment (is example with the tea tree ethereal oil)

Embodiment one: be used to improve looks and the permanent fragrance-lasting natural tea tree ethereal oil nano-emulsion of SPA

Each raw material preferred weight proportioning of tea tree ethereal oil is: tea tree ethereal oil 3, isopropyl myristate 7, sad capric acid polyethyleneglycol glyceride 30, polyglyceryl fatty acid ester 10, triple distillation water 50.

The preparation method of tea tree ethereal oil nano-emulsion (100g is an example with preparation):

1. get among the 100mL conical flask that the 50g tri-distilled water places decontamination, clearly be labeled as water (I) 50g with colored marking pen.2. accurately take by weighing the isopropyl myristate of 7g weight with analytical balance, place another through among 50mL conical flask of decontamination, add the 3g tea tree ethereal oil, above material is ultrasonic mixing in water-bath, and with clear oil phase (1I) 10g that is labeled as of marking pen.3. according to the sad capric acid polyethyleneglycol glyceride (S) that has designed in advance: the ratio of polyglyceryl fatty acid ester (C)=3: 1, accurately take by weighing sad capric acid polyethyleneglycol glyceride of 30g and 10g polyglyceryl fatty acid ester respectively, and both are placed among the clean conical flask of 50mL of the 3rd process decontamination; Add a cover, and this conical flask placed rapidly on the liquid flash mixer, open the liquid flash mixer, and it is transferred to the II shelves, fully mix homogeneously makes it to form emulsifying agent/co-emulsifier (S/C) mixture, behind the 3-5min, the closing liquid flash mixer takes off conical flask, and with clear S/C mixture (III) 40g that is labeled as of marking pen.4. according to oil phase (II): S/C (III): the ratio of water (I)=10%: 40%: 50%, fetch water respectively earlier phase (I) 50g, S/C mixture (III) 40g place among the 4th the clean conical flask of the 200mL through decontamination.5. after above-mentioned water (I), S/C mixture (III) two liquid phases fully being mixed, again under 25 ℃ of conditions of room temperature or in the natural room temperature, put it into and shake in the ultrasonator also about ultrasonic 2min, perhaps under 25 ℃ of conditions of room temperature or in the natural room temperature, start the time constant-temperature magnetic stirring apparatus, with 200rpmmin ^-1Rotating speed magnetic agitation 5min, then, in this container, directly add oil phase (II) 10g again, and with whole system in ultrasonator about ultrasonic 10min, perhaps under 25 ℃ of conditions of room temperature or in the natural room temperature, start the time constant-temperature magnetic stirring apparatus, with 200rpmmin ^-1Rotating speed magnetic agitation 20min.6. close ultrasonator or time constant-temperature magnetic stirring apparatus, take off the 200mL conical flask, observe its outward appearance colourless in this way, limpid transparent, fluidity and good dispersion, obviously visible opalescence person is arranged, be the 100g tea tree ethereal oil nano-emulsion of external preparation for skin.7. this 100g tea tree ethereal oil nano-emulsion is sub-packed in rapidly among the lucifuge glass container of different size, adds a cover, sterilization, packing, and place to preserve among drying, ventilation, the shady and cool room temperature and get final product.8. according to International or National or enterprise's relevant criterion and requirement, stochastic sampling from above-mentioned packing product, and carry out the related system that national departments concerned requires and detect, after waiting to judge or estimating it and meet national relevant regulations and standard, promptly can be used as launch and sell.

* remarks: all implementation steps and operating process are all carried out in the aseptic environments in cleaning, and operator should carry out in strict accordance with sterile working's rules.

Claims (5)

1. one kind is used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy, it is characterized in that the essence oil nanometer breast is to be made by the following weight parts proportion raw material:

Natural essential oil 2-5, isopropyl myristate 5-10, sad capric acid polyethyleneglycol glyceride 25-45, polyglyceryl fatty acid ester 8-15, triple distillation water 45-60.

2. according to claim 1ly a kind ofly be used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy, wherein the preferred weight proportion of essence oil nanometer each raw material of breast is:

Tea tree ethereal oil 3, isopropyl myristate 7, sad capric acid polyethyleneglycol glyceride 30, polyglyceryl fatty acid ester 10, triple distillation water 50.

3. according to claim 1ly a kind ofly be used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy, it is characterized in that described isopropyl myristate and natural essential oil are as oil phase.

4. according to claim 3ly a kind ofly be used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy, it is characterized in that described sad capric acid polyethyleneglycol glyceride and polyglyceryl fatty acid ester as emulsifying agent and co-emulsifier mixture, and weight ratio be 3: 1.

5. according to claim 4ly a kind ofly be used to improve looks and the natural essential oil nano-emulsion of quintessence oil therapy, it is characterized in that it is made up of following weight percentages:

Oil phase 10%; Emulsifying agent and co-emulsifier mixture 40%; Water 50%.

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