CN101829261A - Traditional Chinese medicine for treating psoriasis as well as preparation method and quality control method thereof - Google Patents
Traditional Chinese medicine for treating psoriasis as well as preparation method and quality control method thereof Download PDFInfo
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Abstract
本发明公开了一种治疗银屑病的中药及其制备方法和质量控制方法。该中药以土茯苓、拔葜、秦皮、苦地丁、金银花、连翘、紫草、牡丹皮、赤芍、地黄、栀子、黄芩、土大黄、鳖甲、黄芪等为主要成分；其质量控制方法，包括秦皮、赤芍和栀子的定性测定和浸出物含量测定。该种药物着重清热解毒利湿，辅以凉血，并稍佐滋养阴血和托里透邪之品，则使在皮肤肌腠之热毒得清，湿邪得去，血热得凉，而达到恢复皮肤肌腠正常生理的作用；质量检测控制方法保证了制剂质量检测标准的准确性和先进性，能够有效控制制剂的质量，可作为制剂质量控制和考察工艺的稳定性的指标。
The invention discloses a traditional Chinese medicine for treating psoriasis, a preparation method and a quality control method thereof. The traditional Chinese medicine is mainly composed of Smilax tuckahoe, Rhizoma chinensis, Qinpi, Kudiding, honeysuckle, forsythia, comfrey, moutan cortex, red peony root, rehmannia glutinosa, gardenia, scutellaria baicalensis, rhubarb rhubarb, turtle shell, and astragalus; its quality Control methods include qualitative determination and extract content determination of Qinpi, Radix Paeoniae Rubra and Gardenia. This kind of medicine focuses on clearing away heat, detoxifying and promoting dampness, supplemented by cooling blood, and supplemented with nourishing yin and blood and Tori to remove pathogenic factors, so that the heat and poison in the skin and muscles can be cleared, damp pathogens can be removed, and blood heat can be cooled. To achieve the effect of restoring the normal physiology of the skin, muscles and skin; the quality inspection control method ensures the accuracy and advancement of the preparation quality inspection standard, can effectively control the quality of the preparation, and can be used as an index for preparation quality control and the stability of the inspection process.
Description
Technical field
The present invention relates to a kind of Chinese medicine, psoriatic Chinese medicine of particularly a kind of treatment and preparation method thereof and method of quality control.
Background technology
Psoriasis is commonly called as " psoriasis ", is a kind of chronic, recurrent, refractory skin, clinically is difficult to radical cure." the white arrange ", " psoriasis " that are equivalent to motherland's medical science.The population of China sickness rate reaches 0.7%, and sickness rate has and increases trend year by year in recent years.And exist the male more than the women, and the north is more than south, and the city is higher than the characteristics in rural area.The obstinate refractory of this disease, etiology unknown, pathogeny is not clear and definite as yet, it is generally acknowledged relevant with heredity, infection, immunologic derangement, Nervous and Mental Factors.
The psoriatic medicine of the present systematic treating of doctor trained in Western medicine has preparations such as methotrexate (MTX), acitretin, ciclosporin, Radix Tripterygii Wilfordii, antibiotic.External used medicine has emollient, keatolytics, and the carbamide in the emollient can alleviate epidermal hyperplasia and the symptom of scratching where it itches, the squama after keatolytics can increase by removing, and making other medicines enter cutaneous permeability increases.External corticosteroid treatment psoriasis instant effect, fine to the skin lesion area especially less than patient's effect of body surface area 5%.But drug withdrawal knock-on and local atrophoderma are arranged, telangiectasis, purpura, hormonelike side effect such as purple striae.Because the organ toxicity of the psoriatic medicine various degrees of treatment comprises spinal cord inhibition, liver, nephrotoxicity, teratogenecity and other untoward reaction.Unite and use different mechanism of action, different action target spot and the non-overlapping medicine of untoward reaction can improve curative effect.Drug combination one is coupling between system's medicine, the 2nd, and system's medication and local topical drug combination.In a word, psoriasis be multifactorly cause, the chronic disease of outbreak repeatedly, drug treatment is a lot, curative effect is different, the untoward reaction weight differs.Treat the direction that the safe medicine of psoriasis good effect is still effort from now on and seek.
The traditional Chinese medical science has multiple dialectical and method of treatment to psoriasis.Think that the institute of primary disease sends out, endogenous cause of ill seven emotions fire-transformation or grasp plain heat in blood, the flesh table is pounced in disturbance nutrient blood outward, occlusion of the pores of skin, qi depression to blood stasis, or the surfeit raw meat sends out, incoordination between the spleen and stomach, damp-heat gluing each other, outer transdermal and sending out; Exopathogenic factor is experienced excessive QI (wind heat, rheumatism, wind and cold, wind-dryness) and is fought in skin, or heat-transformation damage channels, or becomes the stasis of blood to become damp and hot heap soil or fertilizer over and around the roots resistance channels, the meromyarian natural fibre line of meat lose moisten support due to.As " all source of disease marquis opinion " what is called: " space between skin and muscles void is subjected to, wind and gas also, blood puckery and can not flourish muscle so also ", and " all be pathogenic wind-dampness, objective in space between skin and muscles, the complex value cold-damp is fought mutually with vim and vigour and is given birth to ".Retention of damp-heat in the interior, the contamination blood vessels, blockage of the vessel is sent out in the skinfold place well so see erythra, and has moistening or sepage is arranged.Hyperactivity of toxic heat, thus see vexed, fever of the body, oliguria with yellow urine.Intense heat in the blood, the absurd row of forcing blood, outside the blood oozing from the body openings or subcuta neous tissue arteries and veins, so the visible petechial hemorrhage in back is scraped in squama stripping in erythra surface, and visible pimple, maculopapule or the patch that differs in size, scarlet or dark red.Excessive QI is remaining so see little itching.Red, the yellow and greasy fur of red tongue, stringy and rolling pulse or sliding number average are damp and hot and the resembling of pyretic toxicity.
Summary of the invention
The purpose of this invention is to provide psoriatic Chinese medicine of a kind of treatment and preparation method thereof and method of quality control.
The technical scheme that the present invention takes is:
The psoriatic Chinese medicine of a kind of treatment, it is to be made by the primary raw material of following weight portion ratio: 10～15 parts of Rhizoma Smilacis Glabraes, pull out 3～10 parts in 5～10 parts of chinaroot greenbriers, 5～10 parts of Cortex Fraxinis, 5～10 parts of Herba Corydalis Bungeanaes, 5～10 parts of Flos Loniceraes, 5～10 parts of Fructus Forsythiaes, 5～10 parts of Radix Arnebiae (Radix Lithospermi)s, 5～10 parts of Cortex Moutans, 5～10 parts of Radix Paeoniae Rubra, 10～15 parts of Radix Rehmanniae, 3～10 parts of Fructus Gardeniaes, 3～10 parts of Radix Scutellariaes, 3～10 parts of Radix Rumiciss, 5～10 parts of Carapax Trionycis (system), 5～10 parts of the Radixs Astragali and Radix Glycyrrhizae.
The psoriatic preparation method of Chinese medicine of above-mentioned treatment, step is as follows:
(1) raw material is handled: whole pulverizing medicinal materials are become micropowders (diameter of particle＜75 μ m); Perhaps earlier to Rhizoma Smilacis Glabrae, pull out and add four quality 6-25 decocting doubly in chinaroot greenbrier, the Radix Astragali and the Radix Glycyrrhizae and boil 2-4 time, filter, it is that the concentrated solution of 0.8-1.35 (50-80 ℃) is standby that filtrate is condensed into relative density, again other medical material Cortex Fraxini, Herba Corydalis Bungeanae, Flos Lonicerae, Fructus Forsythiae, Radix Arnebiae (Radix Lithospermi), Cortex Moutan, Radix Paeoniae Rubra, Radix Rehmanniae, Fructus Gardeniae, Radix Scutellariae, Radix Rumicis and Carapax Trionycis (system) is ground into the thin medicated powder of 60-120 purpose;
(2) pill: the micropowders that step (1) is made is with the binding agent pill, and used micropowders and adhesive consumption mass ratio are 1: 0.8-1.6; Perhaps thin medicated powder that concentrated solution and step (1) are made and binding agent mixing according to a certain percentage, pill, thin medicated powder is thin medicated powder with the mass ratio of concentrated solution, binding agent: (concentrated solution+binding agent)=1: 0.8-1.6, drying, per 10 balls weight 0.6-1.5g.
Above-mentioned binding agent is 0.5-5% (wt%) starch slurry or 0.5-5% (wt%) refined honey.
Medicine of the present invention is that pure Chinese medicine is made, and preparation technology is simple.
Chinese medicine of the present invention is at the psoriasis retention of damp-heat in the interior, hyperactivity of toxic heat, the pathogenic characteristic of intense heat in the blood is with Rhizoma Smilacis Glabrae, to pull out chinaroot greenbrier, Cortex Fraxini, Herba Corydalis Bungeanae, Flos Lonicerae, Fructus Forsythiae, Radix Arnebiae (Radix Lithospermi), Cortex Moutan, Radix Paeoniae Rubra, Radix Rehmanniae, Fructus Gardeniae, Radix Scutellariae, Radix Rumicis, Carapax Trionycis (system), the Radix Astragali, Radix Glycyrrhizae be the prescription therapeutic psoriasis.
Rhizoma Smilacis Glabrae sweet in the mouth, light in the side, property is flat.Return liver,kidney,spleen, stomach warp.Property is opened to loose to fall and is let out." property of medicine is cut usefulness " says it: " oozing dampness removing heat, detoxifcation "; " detailed outline " carries it: " controlling the contracture osteodynia, the malignant boil carbuncle "; " book on Chinese herbal medicine just " claims it: " treat carbuncle, sore throat, remove whole body cold-damp, malignant boil ".Reuse in the event side and be monarch.
Rhizoma Smilacis Chinensis sweet in the mouth, acid, property is flat.Return liver, kidney channel." herbal classic is met former " says its " damp eliminating heat, diuretic "; " property of medicine is wanted in the medical center bun " calls it: " clear small intestine fire is changed bladder water.Control malignant boil, skin infection, insect poison "; It is carried in Jiangxi " Chinese herbology ", and " detoxifcation is dispeled the wind, and is the carbuncle sore key medicine.(controlling) psoriasis.”
The Herba Corydalis Bungeanae bitter in the mouth, cold in nature.GUIXIN, liver, large intestine channel." national Chinese herbal medicine compilation " is loaded with: " heat-clearing and toxic substances removing, promoting blood circulation and detumescence.Cure mainly ... furuncle swelling toxin.”
Cortex Fraxini bitter in the mouth, puckery, cold in nature.Return liver, gallbladder, large intestine, lung meridian.The matter heavily fortified point is held back and is fallen." Shanxi Chinese herbal medicine ": " heat clearing away, dampness make eye bright, dysentery relieving "; South medicine " Chinese herbology " claims it: " (curing mainly) psoriasis.”
Flos Lonicerae sweet in the mouth, little hardship, cold in nature.Return lung, the heart, stomach warp.Body is fragrance gently, clear saturating inside and outside." the SHENGCAO property of medicine fully will " claims it: " eliminating carbuncle cellulitis furunculosis, the dysentery relieving disease is washed chancre, peeling skin heat in blood "; " the Chongqing hall random notes opinion property of medicine " carries it: " wind-fire is damp and hot in the clear network, separates the dirty filthy heresy of pestilence "; " property of medicine general examination " says it: " control the carbuncle scabies.”
Fructus Forsythiae bitter in the mouth, suffering, cold nature.GUIXIN, liver, gallbladder meridian.It is " skin infection must be used " that heat-clearing and toxic substances removing, " medicine origin " carry it; Li Dongyuan claims it: " all menses stagnation of QI that looses are poly-, detumescence." the above five tastes share that to help Rhizoma Smilacis Glabrae heat clearing away, dampness removing, detoxifcation altogether be minister.
Radix Rehmanniae sweet in the mouth, hardship, cold nature.GUIXIN, liver, kidney channel.The matter profit is fallen and is let out." Bencao Congxin " calls it: " yin nourishing is brought down a fever, the removing heat from blood hemopoietic "; " property of medicine is cut usefulness " claims it: " rushing down the heat in the blood ... the skin infection varioliform exanthema is laid equal stress on it ".
Radix Paeoniae Rubra acid, hardship are slightly cold.Return liver, the heart, spleen channel.Go into blood system, hold back and fall and little loosing." the southern regions of the Yunnan Province book on Chinese herbal medicine " claims it: " let out spleen-fire, sending down the abnormal ascending QI, heat in blood is moved back in promoting the circulation of blood "; " book on Chinese herbal medicine is stated record " says it: " dampness removing heat, dissipating blood stasis blood is separated dysphoria with smothery sensation.”
Cortex Moutan acrid in the mouth, hardship, cold nature.GUIXIN, liver, kidney channel.Delicate fragrance is loose and is let out, and goes into blood system." detailed outline " calls it: " with blood, hemopoietic, removing heat from blood, controlling the blood fire second of the three ten-day periods of the hot season, heat clearing away and restlessness relieving ".
The Radix Arnebiae (Radix Lithospermi) bitter in the mouth, cold in nature.GUIXIN, Liver Channel.Go into blood system, can rise and to fall." property of medicine opinion " is referred to as: " controlling malignant boil, sick tinea "; " detailed outline " calls it: " control macule, promoting blood circulation and cooling blood, sharp large intestine.”
The Fructus Gardeniae bitter in the mouth, cold in nature.GUIXIN, stomach, liver, tri-jiao channel.Its property can rise and can fall." herbal classic " calls it: " pathogen in main five, pathogenic heat in the stomach gas, flushed complexion, wine bleb Zha nose, the red leprosy of tuberculoid leprosy, skin infection "; " medicine origin " carries it: " remove heart channel exopathogenic heat one also, remove irritated two also; Go part of the body cavity above the diaphragm housing the heart and lungs deficiency-heat three also; Control wind heat four also "; " property of medicine opinion " says it: " malicious wind reduces phlegm and internal heat ".
The Radix Scutellariae bitter in the mouth, cold in nature.Return lung, the heart, liver, gallbladder, large intestine channel.The light gas of body is clear, can rise to fall." herbal classic " carries it: " intestinal Bleach lets out dysentery, relieves oedema or abdominal distension through diuresis or purgation for main all heat, jaundice, and hematochezia closes, (controlling) malignant boil, cellulitis erosion, acute scleritis "; " book on Chinese herbal medicine just " calls it: " heat of flesh table of dispelling especially is so control macule, scrofula, skin infection, acute conjunctivitis; The heat of the cool part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels can be removed bloody dysentery, and heat is held bladder, the puckery pain of five types of stranguria, large intestine closes knot, has blood in stool, and leaks blood "; " pearl in red water " claims its " getting Fructus Forsythiae then sterilizes ".
Radix Rumicis bitter in the mouth, suffering, cool in nature.Return lung, large intestine channel." interrogating book on Chinese herbal medicine " calls it: " heat clearing away can be applied fire-toxin ", " controlling scabies imitates most "; " detailed outline is picked up any lost article from the road " claims it: " more scabies "; " Hunan medicine will " carries it: " dispelling wind damp eliminating, killing parasites for relieving itching, heat-clearing and toxic substances removing, activating blood circulation, removing blood stasis with potent drugs.Cure mainly ... desquamation, scabies, children's's clear water skin ulcer, swelling of the feet is mashed, the tinea leprosy." above seven flavors share heat clearing and blood cooling, detoxifying, removing dampness, but and cool the blood dissipate blood stasis in case intenseness of heat cause the stasis of blood and prevent because of medication too cold hold back and cause the stasis of blood, can be adjuvant drug.
Radix Astragali sweet in the mouth, slightly warm in nature.Return spleen, lung meridian.Go into edema caused by disorder of QI, can rise and to fall.But merit Tuoli expelling pathogenic factors from the exterior.Essentials of Matea Medica says that it can " real space between skin and muscles rushes down YIN-fire, expelling pathogenic factors from muscles heat "." medicine origin " carries it: " tonifying the lung gas, real fur, eliminating the pathogens from the lung moderate heat, stringy pulse spontaneous perspiration.Be apt to control weakness of the spleen and stomach, the skin infection blood vessels are not all right, expelling the toxic substance from the body with tonic YIN syndrome skin infection." essentials of Matea Medica: " hemopoietic, granulation promoting, evacuation of pus expelling the toxic substance from the body with tonic, carbuncle sore panacea.”
The Carapax Trionycis salty in the mouth, cold nature.Return liver, kidney channel.Hard the diving of matter fallen, and is apt to into cloudy blood." solar corona " claims it: " skin ulcer that disappears swells and pounces on and decrease blood stasis, malaria, acute appendicitis for the gas ， Po Disorder that dehematizes knot, stagnant blood, induced abortion." share the heat clearing away YIN nourishing with the Radix Rehmanniae, in case many product consumption impairment of YIN blood that oozes profit in the side.Share the power that can strengthen its Tuoli expelling pathogenic factors from the exterior with the Radix Astragali.Two flavors are shared, also are adjuvant drug.
The Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription is for making.
All medicines share, and the heat-clearing and toxic substances removing dampness removing is aided with removing heat from blood, and helps the product of yin and blood nourishing and Tuoli expelling pathogenic factors from the exterior slightly emphatically, and the pyretic toxicity at the skin flesh natural fibre line of meat is got clearly, and damp must be gone, and heat in blood gets cool, and reach the effect that recovers skin flesh natural fibre line of meat normal physiological.The compatibility characteristics: reinforcement and elimination in combination, get rid of evils and just do not hinder.
The method of quality control of the psoriatic Chinese medicine of above-mentioned treatment comprises that the qualitative identification of Cortex Fraxini, Radix Paeoniae Rubra, Cortex Moutan and Fructus Gardeniae and extract content measure, and the qualitative identification step is as follows:
(1) gets Chinese medicine 0.5-5g of the present invention, porphyrize, the 20-100ml that adds diethyl ether, reflux 10-30 minute, filter, discard ether, residue adds methanol 10-50ml after flinging to ether, reflux 20-60 minute, filter, residue adds methanol 2-10ml and makes dissolving behind the filtrate evaporate to dryness, as need testing solution; Get the aseculin reference substance, add methanol and make the solution that every 1ml contains the 0.1-0.5mg aseculin, in contrast product solution; Draw need testing solution 1-5 μ l, reference substance solution 1-5 μ l puts respectively on same silica gel g thin-layer plate, with (4-8): (0.5-1.5): chloroform-methanol (0.1-0.8)-formic acid is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color;
(2) get Chinese medicine 2-8g of the present invention, porphyrize, 20-100ml adds diethyl ether, reflux 10-30 minute, filter, discard ether, residue adds methanol 30-100ml after flinging to ether, reflux 20-60 minute, filters, residue adds water 20-50ml and makes its dissolving behind the filtrate evaporate to dryness, extracts 2-4 time the merging n-butanol extracting liquid with the water-saturated n-butanol jolting, evaporate to dryness, residue add methanol 5-10ml makes dissolving, adds the 1-3g neutral alumina, mix thoroughly, drying is put on the neutral alumina post, with ethyl acetate 20-50ml eluting, discard eluent, with 1: 1 ethyl acetate-methanol 30-60ml eluting, collect eluent, evaporate to dryness, residue adds methanol 1-2ml makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains the 1-2mg peoniflorin, in contrast product solution; Draw need testing solution 2-5 μ l, reference substance solution 2-5 μ l, point sample is on same silica gel g thin-layer plate respectively, with (30-50): (2-10): (5-15): chloroform-ethyl acetate (0.1-0.6)-methanol-formic acid is developing solvent, launch, take out, dry, spray is with 2-5% (wt%) vanillin sulfuric acid solution, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
(3) get Chinese medicine 2-8g of the present invention, porphyrize, 20-50ml adds diethyl ether, reflux 20-30 minute, filter, discard ether, residue adds ethyl acetate 20-100ml after flinging to ether, reflux 30-60 minute, filters, filtrate evaporate to dryness, residue add dehydrated alcohol 20-60ml makes its dissolving, adds activated carbon 0.1-0.3g,, reflux 2-15 minute, filter, filtrate evaporate to dryness, residue add methanol 1-5ml makes dissolving, as need testing solution; Other gets the jasminoidin reference substance, adds methanol and makes the solution that every 1ml contains the 1-2mg jasminoidin, in contrast product solution; Draw need testing solution 2-5 μ l, reference substance solution 2-5 μ l, point sample is on same silica gel g thin-layer plate respectively, with (5-15): (3-10): (1-5): ethyl acetate-acetone (0.1-0.8)-formic acid-water is developing solvent, launch, take out, dry, spray is with 5-10% (wt%) ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
Hot dipping under the extract content mensuration photograph ethanol-soluble extractives algoscopy item (" 2005 editions one appendix X A of Chinese pharmacopoeia) measure, make solvent with 75-95% ethanol, extract content must not be less than 20.0% (ww%).
This quality checking and controlling method has guaranteed the accuracy and the advance of quality of the pharmaceutical preparations examination criteria, can effectively control the quality of preparation, can be used as the index of quality of the pharmaceutical preparations control and investigation technology stability.
Above-mentioned qualitative identification step (1) is the discriminating of Cortex Fraxini in the prescription, the preparation method of the preparation technology of analog sample and test liquid has made Cortex Fraxini medical material contrast liquid, has lacked Cortex Fraxini blank liquid in the test, tomographic results shows, in test sample chromatograph, the control medicinal material chromatograph, with the corresponding position of reference substance chromatograph on show the speckle of same color, lacking the Cortex Fraxini blank does not then have this speckle on corresponding position, see Fig. 1.
Above-mentioned qualitative identification step (2) is the discriminating of Radix Paeoniae Rubra, Cortex Moutan in the prescription, all contains peoniflorin in Radix Paeoniae Rubra, the Cortex Moutan, and therefore adopting peoniflorin is contrast, and the two is differentiated.The preparation method of the preparation technology of analog sample and test liquid has made Radix Paeoniae Rubra medical material, Cortex Moutan medical material, has lacked Radix Paeoniae Rubra, Cortex Moutan blank liquid in the test, tomographic results shows, in test sample chromatograph, the control medicinal material chromatograph, with the corresponding position of reference substance chromatograph on show the speckle of same color, Radix Paeoniae Rubra, Cortex Moutan blank then do not have this speckle on corresponding position, see Fig. 2.
Above-mentioned qualitative identification step (3) is the discriminating of Fructus Gardeniae in the prescription.The preparation method of the preparation technology of analog sample and test liquid has made the Fructus Gardeniae medical material, has lacked Fructus Gardeniae blank liquid in the test, tomographic results shows, in test sample chromatograph, the control medicinal material chromatograph, with the corresponding position of reference substance chromatograph on show the speckle of same color, lacking the Fructus Gardeniae blank does not then have this speckle on corresponding position, see Fig. 3.
Description of drawings
Fig. 1 is for differentiating the TLC figure of Cortex Fraxini, and wherein 1. lack three batches of test samples of blank 2. aseculin, the 3. Cortex Fraxini 4.5.6. of Cortex Fraxini;
Fig. 2 is for differentiating the TLC figure of Radix Paeoniae Rubra, Cortex Moutan, wherein 1. lacks Radix Paeoniae Rubra, herds three batches of test samples of blank 2. peoniflorin 3. Radix Paeoniae Rubra, the 4. Cortex Moutan 5.6.7. of Cortex Moutan;
Fig. 3 is for differentiating the TLC figure of Fructus Gardeniae, and wherein 1. lack three batches of test samples of blank 2. jasminoidins, the 3. Fructus Gardeniae 4.5.6. of Fructus Gardeniae.
The specific embodiment
Embodiment 1
The psoriatic Chinese medicine of a kind of treatment, it is to be made by the primary raw material of following weight: Rhizoma Smilacis Glabrae 100g, pull out chinaroot greenbrier 70g, Cortex Fraxini 50g, Herba Corydalis Bungeanae 50g, Flos Lonicerae 50g, Fructus Forsythiae 50g, Radix Arnebiae (Radix Lithospermi) 50g, Cortex Moutan 50g, Radix Paeoniae Rubra 70g, Radix Rehmanniae 100g, Fructus Gardeniae 40g, Radix Scutellariae 40g, Radix Rumicis 40g, Carapax Trionycis (system) 70g, Radix Astragali 70g and Radix Glycyrrhizae 40g.
The psoriatic preparation method of Chinese medicine of above-mentioned treatment, step is as follows: all pulverizing medicinal materials becomes micropowders (diameter of particle＜75 μ m), with 4% starch slurry (medicated powder: starch slurry=1: 1.2wt) pill, drying, the heavy 1g of per 10 balls.
Treat the method for quality control of psoriatic Chinese medicine, comprise qualitative identification and the extract content mensuration of Cortex Fraxini, Radix Paeoniae Rubra and Fructus Gardeniae, the qualitative identification step is as follows:
(1) get Chinese medicine 1.5g of the present invention, porphyrize, the 30ml that adds diethyl ether, reflux 30 minutes filters, and discards ether, and residue is flung to ether, adds methanol 25ml, and reflux 30 minutes filters, and filtrate evaporate to dryness, residue add methanol 10ml makes dissolving, as need testing solution.Get the aseculin reference substance, add methanol and make the solution that every 1ml contains 0.5mg, in contrast product solution.According to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw need testing solution 2 μ l, reference substance solution 1 μ l, putting in same 0.3% sodium carboxymethyl cellulose respectively is on the silica gel g thin-layer plate of adhesive, with chloroform-methanol-6: 1: 0.5 (V: V: V) be developing solvent, launch, take out of formic acid, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color.
(2) get Chinese medicine 5g of the present invention, porphyrize, 50ml adds diethyl ether, reflux 30 minutes filters, and discards ether, residue is flung to ether, adds methanol 50ml, reflux 1 hour, filter, filtrate evaporate to dryness, residue add water 40ml makes its dissolving, extract 3 times with the water-saturated n-butanol jolting, for the first time 40ml, 30ml for the second time, 30ml merges n-butanol extracting liquid, evaporate to dryness for the third time, residue adds methanol 5ml makes dissolving, adds the 2g neutral alumina, mixes thoroughly, drying is put neutral alumina post (100～200 orders, 7g, internal diameter 1.5cm) on, with ethyl acetate 30ml eluting, discard eluent, with ethyl acetate-methanol (1: 1) 50ml eluting, collect eluent, evaporate to dryness, residue add methanol 2ml makes dissolving, as need testing solution.Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains 2mg, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 5 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with chloroform-ethyl acetate-methanol-formic acid 40: 5: 10: 0.2 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(3) get Chinese medicine 5g of the present invention, porphyrize, the 50ml that adds diethyl ether, reflux 30 minutes, filter, discard ether, residue is flung to ether, add ethyl acetate 50ml, reflux 1 hour filters, filtrate evaporate to dryness, residue add dehydrated alcohol 40ml makes its dissolving, adds activated carbon 0.2g,, reflux 10 minutes filters, filtrate evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the jasminoidin reference substance, adds methanol and makes every 1ml and contain 1mg solution, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 3 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with ethyl acetate-acetone-formic acid-water 10: 6: 2: 0.5 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Hot dipping under the extract content mensuration photograph ethanol-soluble extractives algoscopy item (" 2005 editions one appendix X A of Chinese pharmacopoeia) measure, make solvent with 80% ethanol, extract content is 24.32%.
Embodiment 2
The psoriatic Chinese medicine of a kind of treatment, it is to be made by the primary raw material of following weight: Rhizoma Smilacis Glabrae 100g, pull out chinaroot greenbrier 70g, Cortex Fraxini 50g, Herba Corydalis Bungeanae 50g, Flos Lonicerae 50g, Fructus Forsythiae 50g, Radix Arnebiae (Radix Lithospermi) 50g, Cortex Moutan 50g, Radix Paeoniae Rubra 70g, Radix Rehmanniae 100g, Fructus Gardeniae 40g, Radix Scutellariae 40g, Radix Rumicis 40g, Carapax Trionycis (system) 70g, Radix Astragali 70g and Radix Glycyrrhizae 40g.
The psoriatic preparation method of Chinese medicine of above-mentioned treatment, step is as follows: all the medical material superfine powder is broken into micropowders (diameter of particle＜75 μ m), with 4% starch slurry (medicated powder: starch slurry=1: 1.2wt) pill, drying.The heavy 1g of per 10 balls.
Treat the method for quality control of psoriatic Chinese medicine, comprise qualitative identification and the extract content mensuration of Cortex Fraxini, Radix Paeoniae Rubra and Fructus Gardeniae, the qualitative identification step is as follows:
(1) get Chinese medicine 1.5g of the present invention, porphyrize, the 30ml that adds diethyl ether, reflux 30 minutes filters, and discards ether, and residue is flung to ether, adds methanol 25ml, and reflux 30 minutes filters, and filtrate evaporate to dryness, residue add methanol 10ml makes dissolving, as need testing solution.Get the aseculin reference substance, add methanol and make the solution that every 1ml contains 0.5mg, in contrast product solution.According to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw need testing solution 2 μ l, reference substance solution 1 μ l, putting in same 0.3% sodium carboxymethyl cellulose respectively is on the silica gel g thin-layer plate of adhesive, with chloroform-methanol-6: 1: 0.5 (V: V: V) be developing solvent, launch, take out of formic acid, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color.
(2) get Chinese medicine 5g of the present invention, porphyrize, 50ml adds diethyl ether, reflux 30 minutes filters, and discards ether, residue is flung to ether, adds methanol 50ml, reflux 1 hour, filter, filtrate evaporate to dryness, residue add water 40ml makes its dissolving, extract 3 times with the water-saturated n-butanol jolting, for the first time 40ml, 30ml for the second time, 30ml merges n-butanol extracting liquid, evaporate to dryness for the third time, residue adds methanol 5ml makes dissolving, adds the 2g neutral alumina, mixes thoroughly, drying is put neutral alumina post (100～200 orders, 7g, internal diameter 1.5cm) on, with ethyl acetate 30ml eluting, discard eluent, with ethyl acetate-methanol (1: 1) 50ml eluting, collect eluent, evaporate to dryness, residue add methanol 2ml makes dissolving, as need testing solution.Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains 2mg, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 5 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with chloroform-ethyl acetate-methanol-formic acid 40: 5: 10: 0.2 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(3) get Chinese medicine 5g of the present invention, porphyrize, the 50ml that adds diethyl ether, reflux 30 minutes, filter, discard ether, residue is flung to ether, add ethyl acetate 50ml, reflux 1 hour filters, filtrate evaporate to dryness, residue add dehydrated alcohol 40ml makes its dissolving, adds activated carbon 0.2g,, reflux 10 minutes filters, filtrate evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the jasminoidin reference substance, adds methanol and makes every 1ml and contain 1mg solution, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 3 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with ethyl acetate-acetone-formic acid-water 10: 6: 2: 0.5 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Hot dipping under the extract content mensuration photograph ethanol-soluble extractives algoscopy item (" 2005 editions one appendix X A of Chinese pharmacopoeia) measure, make solvent with 80% ethanol, extract content is 23.56%.
Embodiment 3
The psoriatic Chinese medicine of a kind of treatment, it is to be made by the primary raw material of following weight: Rhizoma Smilacis Glabrae 100g, pull out chinaroot greenbrier 70g, Cortex Fraxini 50g, Herba Corydalis Bungeanae 50g, Flos Lonicerae 50g, Fructus Forsythiae 50g, Radix Arnebiae (Radix Lithospermi) 50g, Cortex Moutan 50g, Radix Paeoniae Rubra 70g, Radix Rehmanniae 100g, Fructus Gardeniae 40g, Radix Scutellariae 40g, Radix Rumicis 40g, Carapax Trionycis (system) 70g, Radix Astragali 70g and Radix Glycyrrhizae 40g.
The psoriatic preparation method of Chinese medicine of above-mentioned treatment, step is as follows: (1) to Rhizoma Smilacis Glabrae, pull out the decocting that adds 12 times of four quality in chinaroot greenbrier, the Radix Astragali and the Radix Glycyrrhizae and boil 2 times, filter, filtrate is condensed into the concentrated solution that relative density is 1.0 (60 ℃), standby; (2) other medical material Cortex Fraxini, Herba Corydalis Bungeanae, Flos Lonicerae, Fructus Forsythiae, Radix Arnebiae (Radix Lithospermi), Cortex Moutan, Radix Paeoniae Rubra, Radix Rehmanniae, Fructus Gardeniae, Radix Scutellariae, Radix Rumicis and Carapax Trionycis (system) were ground into the fine powder of 100 mesh sieves; (3) fine powder and 0.3% (wt%) the starch slurry 50ml mixing that makes of the concentrated solution that step (1) is made, step (2), pill, drying.The heavy 1g of per 10 balls.
Treat the method for quality control of psoriatic Chinese medicine, comprise qualitative identification and the extract content mensuration of Cortex Fraxini, Radix Paeoniae Rubra and Fructus Gardeniae, the qualitative identification step is as follows:
(1) get Chinese medicine 1.2g of the present invention, porphyrize, the 30ml that adds diethyl ether, reflux 30 minutes filters, and discards ether, and residue is flung to ether, adds methanol 25ml, and reflux 30 minutes filters, and filtrate evaporate to dryness, residue add methanol 10ml makes dissolving, as need testing solution.Get the aseculin reference substance, add methanol and make the solution that every 1ml contains 0.5mg, in contrast product solution.According to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw need testing solution 2 μ l, reference substance solution 1 μ l, putting in same 0.3% sodium carboxymethyl cellulose respectively is on the silica gel g thin-layer plate of adhesive, with chloroform-methanol-6: 1: 0.5 (V: V: V) be developing solvent, launch, take out of formic acid, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color.
(2) get Chinese medicine 4g of the present invention, porphyrize, 50ml adds diethyl ether, reflux 30 minutes filters, and discards ether, residue is flung to ether, adds methanol 50ml, reflux 1 hour, filter, filtrate evaporate to dryness, residue add water 40ml makes its dissolving, extract 3 times with the water-saturated n-butanol jolting, for the first time 40ml, 30ml for the second time, 30ml merges n-butanol extracting liquid, evaporate to dryness for the third time, residue adds methanol 5ml makes dissolving, adds the 2g neutral alumina, mixes thoroughly, drying is put neutral alumina post (100～200 orders, 7g, internal diameter 1.5cm) on, with ethyl acetate 30ml eluting, discard eluent, with ethyl acetate-methanol (1: 1) 50ml eluting, collect eluent, evaporate to dryness, residue add methanol 2ml makes dissolving, as need testing solution.Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains 2mg, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 5 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with chloroform-ethyl acetate-methanol-formic acid 40: 5: 10: 0.2 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(3) get Chinese medicine 4g of the present invention, porphyrize, the 50ml that adds diethyl ether, reflux 30 minutes, filter, discard ether, residue is flung to ether, add ethyl acetate 50ml, reflux 1 hour filters, filtrate evaporate to dryness, residue add dehydrated alcohol 40ml makes its dissolving, adds activated carbon 0.2g,, reflux 10 minutes filters, filtrate evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the jasminoidin reference substance, adds methanol and makes every 1ml and contain 1mg solution, in contrast product solution.According to thin layer chromatography (" 2005 editions one appendix VI B of Chinese pharmacopoeia) test, draw need testing solution 3 μ l, reference substance solution 2 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with ethyl acetate-acetone-formic acid-water 10: 6: 2: 0.5 (V: V: V: V) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Hot dipping under the extract content mensuration photograph ethanol-soluble extractives algoscopy item (" 2005 editions one appendix X A of Chinese pharmacopoeia) measure, make solvent with 80% ethanol, extract content is 26.48%.
Embodiment 4
The psoriatic Chinese medicine of a kind of treatment, it is to be made by the primary raw material of following weight: Rhizoma Smilacis Glabrae 150g, pull out chinaroot greenbrier 50g, Cortex Fraxini 100g, Herba Corydalis Bungeanae 100g, Flos Lonicerae 100g, Fructus Forsythiae 100g, Radix Arnebiae (Radix Lithospermi) 100g, Cortex Moutan 100g, Radix Paeoniae Rubra 50g, Radix Rehmanniae 150g, Fructus Gardeniae 100g, Radix Scutellariae 30g, Radix Rumicis 300g, Carapax Trionycis (system) 100g, Radix Astragali 100g and Radix Glycyrrhizae 100g.
The psoriatic preparation method of Chinese medicine of above-mentioned treatment, step is as follows: (1) to Rhizoma Smilacis Glabrae, pull out the decocting that adds 20 times of four quality in chinaroot greenbrier, the Radix Astragali and the Radix Glycyrrhizae and boil 3 times, filter, filtrate is condensed into the concentrated solution that relative density is 1.2 (80 ℃), standby; (2) other medical material Cortex Fraxini, Herba Corydalis Bungeanae, Flos Lonicerae, Fructus Forsythiae, Radix Arnebiae (Radix Lithospermi), Cortex Moutan, Radix Paeoniae Rubra, Radix Rehmanniae, Fructus Gardeniae, Radix Scutellariae, Radix Rumicis and Carapax Trionycis (system) were ground into 120 purpose fine powders; (3) fine powder and 0.1% (wt%) the starch slurry 55ml mixing that makes of the concentrated solution that step (1) is made, step (2), pill, drying, the heavy 1g of per 10 balls.
The method of quality control of the psoriatic Chinese medicine of above-mentioned treatment comprises that the qualitative identification of Cortex Fraxini, Radix Paeoniae Rubra and Fructus Gardeniae and extract content measure, and the qualitative identification step is as follows:
(1) get Chinese medicine 1g of the present invention, porphyrize, the 100ml that adds diethyl ether, reflux 30 minutes filters, and discards ether, and residue adds methanol 25ml after flinging to ether, and reflux 60 minutes filters, and residue adds methanol 10ml and makes dissolving behind the filtrate evaporate to dryness, as need testing solution; Get the aseculin reference substance, add methanol and make the solution that every 1ml contains the 0.5mg aseculin, in contrast product solution; Draw need testing solution 1 μ l, reference substance solution 1 μ l, putting in same 0.3% (wt%) sodium carboxymethyl cellulose respectively is on the silica gel g thin-layer plate of adhesive, with chloroform-methanol of 6: 1: 0.5-formic acid is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color;
(2) get Chinese medicine 3g of the present invention, porphyrize, 100ml adds diethyl ether, reflux 30 minutes filters, and discards ether, residue adds methanol 100ml after flinging to ether, and reflux 60 minutes filters, residue adds water 50ml and makes its dissolving behind the filtrate evaporate to dryness, extracts 3 times with the water-saturated n-butanol jolting, uses 20-40ml for the first time, 20-30ml for the second time, 20-30ml merges n-butanol extracting liquid for the third time, evaporate to dryness, residue add methanol 5-10ml makes dissolving, adds the 3g neutral alumina, mix thoroughly, drying is put neutral alumina post (100～200 orders, 5-10g, internal diameter 1.5cm) on, with ethyl acetate 50ml eluting, discard eluent, with 1: 1 ethyl acetate-methanol 60ml eluting, collect eluent, evaporate to dryness, residue add methanol 2ml makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains the 2mg peoniflorin, in contrast product solution; Draw need testing solution 5 μ l, reference substance solution 5 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% (wt%) sodium carboxymethyl cellulose respectively, with 40: 5: 10: chloroform-ethyl acetate of 0.2-methanol-formic acid was developing solvent, launch, take out, dry, spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
(3) get Chinese medicine 3g of the present invention, porphyrize, 50ml adds diethyl ether, reflux 30 minutes filters, and discards ether, residue adds ethyl acetate 40ml after flinging to ether, and reflux 60 minutes filters, filtrate evaporate to dryness, residue add dehydrated alcohol 60ml makes its dissolving, adds activated carbon 0.3g,, reflux 15 minutes filters, filtrate evaporate to dryness, residue add methanol 5ml makes dissolving, as need testing solution; Other gets the jasminoidin reference substance, adds methanol and makes the solution that every 1ml contains the 2mg jasminoidin, in contrast product solution; Draw need testing solution 5 μ l, reference substance solution 5 μ l, point sample is on the silica gel g thin-layer plate of adhesive in same 0.3% sodium carboxymethyl cellulose respectively, with 10: 6: 2: ethyl acetate-acetone of 0.5-formic acid-water was developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
Hot dipping under the extract content mensuration photograph ethanol-soluble extractives algoscopy item (" 2005 editions one appendix X A of Chinese pharmacopoeia) measure, make solvent with 80% ethanol, extract content is 26.85%.
Embodiment 5
Chinese medicine clinical observation of the present invention is summed up:
Use Chinese medicine of the present invention 60 routine psoriatics have been carried out the clinical observation summary, obtained clinical efficacy preferably.
Clinical physical data: 60 examples are in January, the 2007-2007 year 12 moon gates example of diagnosing a disease, male 40 examples, and women 20 examples, at minimum 20 years old of age, maximum 60 years old, 37 years old mean age, the course of disease is the longest 30 years, and was the shortest 2 months, average 6.5 years.
Diagnostic criteria: meet diagnostic criteria of doctor trained in Western medicine psoriasis vulgaris and Chinese medical discrimination standard (belonging to retention of damp-heat in the interior, the blood-heat syndrome of hyperactivity of toxic heat, flourishing person).
Get rid of case: gestation or women breast-feeding their children, to the ingredient allergy sufferers, joint type, pustule type, erythrodermic psoriasis, take alclometasone diproionate in nearly 2 weeks orally and/or took the retinoid medicine orally in 1 week or anabolic agent is crossed in external, be associated with serious primary disease such as cardiovascular, cerebrovascular, liver, kidney and hemopoietic system, the psychotic.
Therapeutic Method: oral Chinese medicine of the present invention (Chinese medicine that embodiment 1 makes).1 6g, 2 times on the one.4 weeks were 1 course of treatment, took for 3 courses of treatment.
Curative effect determinate standard: carry out efficacy determination with reference to the PASI point system.Standards of grading are as follows:
The scoring of skin lesion area:
The skin lesion area accounts for body surface area percentage ratio (%) mark
0 0
＜10 1
10-29 2
30-49 3
50-69 4
70-89 5
＞90 6
The erythema scoring:
The erythema mark
No erythema 0
The erythema color is light or dark 1
Skin lesion redness 2
Skin lesion scarlet 3
The squama scoring:
The squama mark
No squama 0
As seen squama 1
Squama thicker 2
Squama piles up 3
Soak into scoring:
Soak into mark
Do not have and soak into plump 0
Slightly exceed surface, outward appearance does not show 1
Obviously exceed surface, moderate is soaked into hardness 2
Moderate plumpness or lichenization 3
Horizon method computing formula=[(integration-treatment back integration before the treatment) the preceding integration of ÷ treatment] * 100% not.
Recovery from illness: integration descends 〉=90%.
Produce effects: integration decline 60-89%.
Effectively: integration decline 30-59%.
Invalid: integration descends＜30%.
Therapeutic effect:
In 60 examples, 41 examples of fully recovering account for 68.3%; Produce effects 12 examples account for 20.0%; Effective 4 examples account for 6.7%; Invalid 3 examples account for 5.0%, total effective rate 88.3%.
Untoward reaction: do not have obviously poison pair effect, laxativeness appears in individual patient.
Embodiment 6
Chinese medicine clinical observation of the present invention is summed up:
Use Chinese medicine of the present invention 60 routine psoriatics have been carried out the clinical observation summary, obtained clinical efficacy preferably.
Clinical physical data: 60 examples are in January, the 2007-2007 year 12 moon gates example of diagnosing a disease, male 38 examples, and women 22 examples, at minimum 20 years old of age, maximum 60 years old, 36 years old mean age, the course of disease is the longest 31 years, and was the shortest 2.5 months, average 6.8 years.
Diagnostic criteria: with embodiment 5.
Get rid of case: with embodiment 5.
Therapeutic Method: oral Chinese medicine of the present invention (Chinese medicine that embodiment 3 makes).1 5g, 2 times on the one.4 weeks were 1 course of treatment, took for 3 courses of treatment.
Curative effect determinate standard: with embodiment 5.
Therapeutic effect:
In 60 examples, 39 examples of fully recovering account for 65.0%; Produce effects 14 examples account for 21.7%; Effective 3 examples account for 5.0%; Invalid 4 examples account for 6.6%, total effective rate 86.7%.
Untoward reaction: do not have obviously poison pair effect, laxativeness appears in individual patient.
Claims (6)
1. treat psoriatic Chinese medicine for one kind, it is characterized in that it is to be made by the primary raw material of following weight portion ratio: 10～15 parts of Rhizoma Smilacis Glabraes, pull out 3～10 parts in 5～10 parts of chinaroot greenbriers, 5～10 parts of Cortex Fraxinis, 5～10 parts of Herba Corydalis Bungeanaes, 5～10 parts of Flos Loniceraes, 5～10 parts of Fructus Forsythiaes, 5～10 parts of Radix Arnebiae (Radix Lithospermi)s, 5～10 parts of Cortex Moutans, 5～10 parts of Radix Paeoniae Rubra, 10～15 parts of Radix Rehmanniae, 3～10 parts of Fructus Gardeniaes, 3～10 parts of Radix Scutellariaes, 3～10 parts of Radix Rumiciss, 5～10 parts of Carapax Trionycis, 5～10 parts of the Radixs Astragali and Radix Glycyrrhizae.
2. according to the psoriatic preparation method of Chinese medicine of the described treatment of claim 1, it is characterized in that step is as follows:
(1) raw material is handled: whole pulverizing medicinal materials are become micropowders; Perhaps earlier to Rhizoma Smilacis Glabrae, pull out and add four quality 6-25 decocting doubly in chinaroot greenbrier, the Radix Astragali and the Radix Glycyrrhizae and boil 2-4 time, filter, it is that the concentrated solution of 0.8-1.35 is standby that filtrate is condensed into relative density, again other medical material Cortex Fraxini, Herba Corydalis Bungeanae, Flos Lonicerae, Fructus Forsythiae, Radix Arnebiae (Radix Lithospermi), Cortex Moutan, Radix Paeoniae Rubra, Radix Rehmanniae, Fructus Gardeniae, Radix Scutellariae, Radix Rumicis and turtle ' s carapace powder is broken into thin medicated powder;
(2) pill: the micropowders that step (1) is made is with the binding agent pill; Perhaps thin medicated powder that concentrated solution and step (1) are made and binding agent mixing pill according to a certain percentage.
3. according to the psoriatic preparation method of Chinese medicine of the described treatment of claim 2, it is characterized in that micropowders that step (2) is used and adhesive consumption mass ratio are 1: 0.8-1.6; The mass ratio of thin medicated powder and concentrated solution, binding agent is thin medicated powder: (concentrated solution+binding agent)=1: 0.8-1.6; Used binding agent is 0.5-5% starch slurry or 0.5-5% refined honey.
4. according to the method for quality control of the psoriatic Chinese medicine of the described treatment of claim 1, comprise qualitative identification and the extract content mensuration of Cortex Fraxini, Radix Paeoniae Rubra, Cortex Moutan and Fructus Gardeniae, it is characterized in that the qualitative identification step is as follows:
(1) it is an amount of to get Chinese medicine of the present invention, porphyrize, the 20-100ml that adds diethyl ether, reflux 10-30 minute, filter, discard ether, residue adds methanol 10-50ml after flinging to ether, reflux 20-60 minute, filter, residue adds methanol 2-10ml and makes dissolving behind the filtrate evaporate to dryness, as need testing solution; Get the aseculin reference substance, add methanol and make the solution that every 1ml contains the 0.1-0.5mg aseculin, in contrast product solution; Draw need testing solution 1-5 μ l, reference substance solution 1-5 μ l puts respectively on same silica gel g thin-layer plate, with (4-8): (0.5-1.5): chloroform-methanol (0.1-0.8)-formic acid is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color;
(2) it is an amount of to get Chinese medicine of the present invention, porphyrize, and 20-100ml adds diethyl ether, reflux 10-30 minute, filter, discard ether, residue adds methanol 30-100ml after flinging to ether, reflux 20-60 minute, filters, residue adds water 20-50ml and makes its dissolving behind the filtrate evaporate to dryness, extracts 2-4 time the merging n-butanol extracting liquid with the water-saturated n-butanol jolting, evaporate to dryness, residue add methanol 5-10ml makes dissolving, adds the 1-3g neutral alumina, mix thoroughly, drying is put on the neutral alumina post, with ethyl acetate 20-50ml eluting, discard eluent, with 1: 1 ethyl acetate-methanol 30-60ml eluting, collect eluent, evaporate to dryness, residue adds methanol 1-2ml makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds methanol and makes the solution that every 1ml contains the 1-2mg peoniflorin, in contrast product solution; Draw need testing solution 2-5 μ l, reference substance solution 2-5 μ l, point sample is on same silica gel g thin-layer plate respectively, with (30-50): (2-10): (5-15): chloroform-ethyl acetate (0.1-0.6)-methanol-formic acid is developing solvent, launch, take out, dry, spray is with 2-5% vanillin sulfuric acid solution, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
(3) it is an amount of to get Chinese medicine of the present invention, porphyrize, and 20-50ml adds diethyl ether, reflux 20-30 minute, filter, discard ether, residue adds ethyl acetate 20-100ml after flinging to ether, reflux 30-60 minute, filters, filtrate evaporate to dryness, residue add dehydrated alcohol 20-60ml makes its dissolving, adds activated carbon 0.1-0.3g,, reflux 2-15 minute, filter, filtrate evaporate to dryness, residue add methanol 1-5ml makes dissolving, as need testing solution; Other gets the jasminoidin reference substance, adds methanol and makes the solution that every 1ml contains the 1-2mg jasminoidin, in contrast product solution; Draw need testing solution 2-5 μ l, reference substance solution 2-5 μ l, point sample is on same silica gel g thin-layer plate respectively, with (5-15): (3-10): (1-5): ethyl acetate-acetone (0.1-0.8)-formic acid-water is developing solvent, launch, take out, dry, spray is with the 5-10% ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
5. according to the method for quality control of the psoriatic Chinese medicine of the described treatment of claim 4, it is characterized in that step (1) gets that dose is 0.5-5g among the present invention; Step (2) gets that dose is 2-8g among the present invention; Step (3) gets that dose is 2-8g among the present invention.
6. according to the method for quality control of the psoriatic Chinese medicine of the described treatment of claim 4, it is characterized in that extract content is measured and made solvent with 75-95% ethanol, extract content must not be less than 20.0%.
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| US9066974B1 (en) | 2010-11-13 | 2015-06-30 | Sirbal Ltd. | Molecular and herbal combinations for treating psoriasis |
| US9095606B1 (en) * | 2010-11-13 | 2015-08-04 | Sirbal Ltd. | Molecular and herbal combinations for treating psoriasis |
| CN105663464A (en) * | 2016-01-21 | 2016-06-15 | 任同亮 | Formula for treating psoriasis and preparation method |
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| CN101496874A (en) * | 2009-03-07 | 2009-08-05 | 马永兵 | Medicament for treating psoriasis and preparation method thereof |
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2010
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| CN1824203A (en) * | 2005-12-22 | 2006-08-30 | 夏金陵 | Heat clearing toxin resolving medicine for treating psora and its preparation method |
| CN101244221A (en) * | 2007-02-13 | 2008-08-20 | 汪建国 | Composition for external application and preparation method |
| CN101264288A (en) * | 2008-04-07 | 2008-09-17 | 张志英 | Orally-administered Chinese medicine for treating psoriasis and preparation thereof |
| CN101375953A (en) * | 2008-09-23 | 2009-03-04 | 刘志新 | Chinese medicine for treating psoriasis |
| CN101496874A (en) * | 2009-03-07 | 2009-08-05 | 马永兵 | Medicament for treating psoriasis and preparation method thereof |
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| US20160136222A1 (en) * | 2010-11-13 | 2016-05-19 | Sirbal, Ltd. | Molecular and Herbal Combinations for Treating Psoriasis |
| US9662363B2 (en) | 2010-11-13 | 2017-05-30 | Sirbal Ltd. | Molecular and herbal combinations for treating psoriasis |
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| US20160113983A1 (en) * | 2010-11-13 | 2016-04-28 | Sirbal, Ltd. | Molecular and Herbal Combinations for Treating Psoriasis |
| US9662365B2 (en) * | 2010-11-13 | 2017-05-30 | Sirbal Ltd. | Molecular and herbal combinations for treating psoriasis |
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| CN110749681A (en) * | 2019-11-19 | 2020-02-04 | 山东省中医药研究院 | A kind of quality evaluation method of traditional Chinese medicine licorice and its application |
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