CN101810201A - Biological preparation for preventing and treating nest insects - Google Patents

Abstract

The invention relates to a biological preparation for preventing and treating nest insects, which is prepared from the following ingredients in percentage by weight: 5 percent to 30 percent of thuringiensis, 20 to 40 percent of bait and 30 to 75 percent of auxiliary materials. The biological preparation can be used for trapping and killing nest insects in bee colonies through being spread at the bottom of a bee case, in seams of the inner wall of a case body and egg laying parts of nest insect female moths. The biological preparation can also be prepared into aqueous suspension to be sprayed on the honeycombs, and the honeycombs are stored after being dried, so the nest insects in the honeycombs can be poisoned and killed. The invention can trap, kill and poison the nest insect, has no toxic or harmful effect on bees and human bodies, and has no pollution on bee products.

Description

A biological agent for preventing broodworm

technical field

The invention relates to a biological agent for preventing and controlling brood insects, and the prevention and control method is safe and environment-friendly, and has no pollution to bee products.

Background technique

Broodworm is one of the most important enemies of bees, and the annual damage caused by the United States alone is much higher than 5 million US dollars. my country is a big beekeeping country in the world, so it is not difficult to calculate the loss caused by broodworm to my country's beekeeping industry will be even greater. Broodworms are unique in their ability to metabolize beeswax, and as such, they live exclusively in the wild or in captive bee colonies, and are rarely found elsewhere. Broodworms use the honeycomb as food, eat away at the comb, drill tunnels in the comb, spin silk and form cocoons, endanger the reproductive ability of the bee colony, and cause the death of bee larvae. In severe cases, the whole colony of bees will abandon the nest and fly away Beekeeping causes irreparable damage. They are harmful to both Apis cerana and Apis mellifera, especially to the former than to the latter. In addition to harming the bees in the hive, broodworms also cause devastating damage to the stored combs. Most beekeepers save the empty combs every year for backup or use in the next year, which will attract female broodworms. The moths lay eggs here, and the hatched broodworms rely on the comb to reproduce in large quantities; usually within 2-4 months, they will almost completely abandon the stored comb due to being eaten away, which shows the extent of its harm. big. When the damage to bee colonies is relatively light, broodworms can be stabbed to death manually; when severe, beekeepers often use chemical drugs for prevention and control, or use physical methods, such as freezing or water immersion. Apparently, chemical methods are easy to pollute bee products. Although physical control methods are harmless, they are time-consuming and laborious or require expensive equipment. As a biological agent, Bacillus thuringiensis (Bt) is highly praised by people. Bt contains insecticidal Gene, such as the patent with publication number WO/2009/088737. However, most of the Bt preparations are used in pesticides, and the main targets are crop pests, which often contain chemical additives or compound drugs that can cause disease or even cancer, such as the patent with publication number WO/2008/092759.

Contents of the invention

In order to meet the needs of bee colonies to prevent and control brood insects and avoid polluting bee products, the invention provides a biological agent for biological control of brood insects, which is controlled by trapping and poisoning methods.

The technical solution adopted by the present invention to solve its technical problems is: provide a kind of biological agent for preventing and treating nest insects, which is composed of Bacillus thuringiensis, bait and auxiliary materials, and the weight percentages are respectively 5%-30%, 20-40% and 30- 75%. The virulence titer of Bacillus thuringiensis is 200-16000IU/mg; the bait is selected from old honeycomb, wax shavings, milk powder, soybean powder and yeast powder; the auxiliary materials are selected from flour, oat flour, corn flour, soluble starch and wheat bran. The method of trapping and killing is used for prevention and control. The former is to place the biological agent in the bottom of the beehive, the gap on the inner wall of the box, and the place where the brood moth female moth lays eggs; the latter is to prepare the biological agent into an aqueous suspension. Then spray it on the honeycomb to ensure that both the wall and the bottom of the honeycomb can come into contact with the medicine, then place the honeycomb in a cool and ventilated place, and store it after drying; before using the honeycomb, wash it with clean water The honeycomb is used after the water is removed by a honey shaker.

The beneficial effects of the present invention are that the use of biological agents to prevent broodworm can reduce the use of chemical drugs, avoid polluting bees and beekeepers from being harmed by chemical drugs; and use the food that broodworm likes as bait to induce broodworm to eat Ingest the insecticidal substance Bacillus thuringiensis at the same time, so as to achieve the effect of trapping and killing broodworm. In addition, the booby trap method only needs to sprinkle the medicine in the beehive, so the operation is simple and easy, and it saves time and effort compared with chemical and physical methods. Because brood moth females like to lay eggs in the wax chips at the bottom of the beehive and in the gaps on the inner wall of the beehive, the medicine is sprinkled at these positions. Do not directly contact bee products, thereby avoiding contamination of bee products. The invention can not only trap and kill broodworms in bee colonies, but also can protect idle spare combs by poisoning. Broodworms are easy to concentrate on destroying the stored honeycombs. Using the present invention, the medicine is sprayed before saving the nests, so that the broodworms will die after eating a very small amount of honeycombs, thereby avoiding the destruction of a large amount of stored honeycombs by nestworms; Before using the honeycomb sprayed with these medicines, it can be washed with clean water, and then the water and residual preparations can be removed by a honey shaker; therefore, the method of the present invention is safer and more environmentally friendly than the previous control methods, especially avoiding the contamination of the preparations in bee products. Residues, to prevent residual drugs from harming bees and threatening human health.

Detailed ways

Example 1:

The Bt is fermented in a liquid state, the active ingredient is collected by centrifugation, and the dry powder is obtained after freeze-drying, and its toxicity potency is 8000 IU/mg. Mix the dry powder of Bacillus thuringiensis, old honeycomb, soybean powder, soluble starch and wheat bran according to different weights and grind them evenly. See Table 1 for the specific ratio.

Table 1 The ratio of biological preparations in each group

Example 2:

Get 5g preparations of different test groups in Example 1 and sprinkle them into the gap between the bottom of the beehive and the inner wall of the box, and the mixture of bait and auxiliary materials without adding Bt is used as a control group, and each group is inserted with 20 2-3 day-old broodworms , Set 3 groups of repetitions, calculate the mortality rate of the broodworm after 6 days; the statistics show that the mortality rate of the broodworm is 90%-100%. The amount of preparation for each group can be properly adjusted according to the instar size and population density.

Example 3:

Bacteria detection was carried out on the experimental bee colonies involved in Example 2, the honey and bee pollen therein were collected respectively, and 10-fold gradient dilution was carried out with sterilized water, and 100 μL of the diluted solution was respectively drawn and coated on a solid nutrient medium, at 30°C, After culturing overnight, no Bt was isolated.

Example 4:

Utilize clear water to dilute 100 times of preparations of equal mass of different test groups in Example 1, make water suspension, spray 35mL medicinal liquid on each empty nest spleen, be placed in ventilated and cool place to dry.

Example 5:

Insert 2-3 day-old nest worms on the dried nest spleen in Example 4, 20 in each group, and set 3 groups of repetitions; the control group was sprayed with the same volume of bait and auxiliary material mixture suspension without adding Bt; After 6 days, the mortality rate of the broodworm was calculated; it was found that the mortality rate of the broodworm was 95% to 100%. The amount of preparation for each honeycomb can be adjusted appropriately according to the characteristics of the utensil for storing the honeycomb. If the airtightness of the utensil is good, the dosage can be reduced, otherwise, the concentration of the medicine in the aqueous suspension can be increased.

Embodiment 6:

The honeycomb involved in Example 5 was washed 2 times with clear water, and then put it back into the bee colony after removing the water and drying it. After 3 days, the honey and bee pollen in the honeycomb were tested for bacteria. Bt was not isolated by the bacterial culture method.

Claims (5)

1. biologic product of preventing and treating the nest worm, it is characterized in that: biologic product is made up of thuringiensis, bait and auxiliary material, and percentage by weight is respectively 5%-30%, 20-40% and 30-75%.

2. biologic product according to claim 1 is characterized in that: bait is selected from old honeycomb, wax bits, milk powder, bean powder and dusty yeast.

3. biologic product according to claim 1 is characterized in that: auxiliary material is selected from flour, oat meal, corn flour, soluble starch and wheat bran.

4. the trapping method of control nest worm is characterized in that: the described biologic product of claim 1 is put in the slit of bottom board, cabinet wall and the place of laying eggs of the female moth of nest worm.

5. prevent and treat the poisoning method of nest worm, it is characterized in that: the described biologic product of claim 1 is formulated as aqueous suspensions, then it is sprayed on the honeycomb, guarantee that lair wall and lair bottom all can touch medicament, again honeycomb is positioned over the shady and cool ventilation place, treats to preserve after its drying; Before using these honeycombs, utilize clear water washing honeycomb, after removing moisture, uses by honey extractor again.