CN101799383B - Hot melt adhesive micro homogenizer - Google Patents
Hot melt adhesive micro homogenizer Download PDFInfo
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- CN101799383B CN101799383B CN2010101366731A CN201010136673A CN101799383B CN 101799383 B CN101799383 B CN 101799383B CN 2010101366731 A CN2010101366731 A CN 2010101366731A CN 201010136673 A CN201010136673 A CN 201010136673A CN 101799383 B CN101799383 B CN 101799383B
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Abstract
热熔胶微量组织匀浆器，它涉及一种微小生物组织匀浆器。本发明解决了现有的微小生物组织匀浆器存在破碎效果不好、处理样品用量多、破碎时间长以及易使被处理样品快速升温从而破坏某些样品中某些成分的问题。本发明所述热熔胶微量组织匀浆器由管体(1)和插装在管体(1)内的槌体(2)构成，所述管体(1)由热熔胶材料制成，所述槌体(2)是不锈钢槌体。管体(1)和不锈钢槌体(2)之间的缝隙接近于零从而达到对微小生物如植物病原线虫，昆虫病原线虫及其他生物和组织的匀浆效果。本发明主要适用于农、林、医等生物和生命科学实验室以及检测站等。
A hot melt adhesive micro-tissue homogenizer, which relates to a micro-biological tissue homogenizer. The present invention solves the problems of existing micro-biological tissue homogenizers, such as poor crushing effect, large amount of sample processing, long crushing time, and easy rapid temperature rise of the processed sample, thereby destroying certain components in certain samples. The hot melt adhesive micro-tissue homogenizer of the present invention is composed of a tube body (1) and a hammer body (2) inserted in the tube body (1), the tube body (1) is made of hot melt adhesive material, and the hammer body (2) is a stainless steel hammer body. The gap between the tube body (1) and the stainless steel hammer body (2) is close to zero, thereby achieving a homogenization effect on micro-organisms such as plant pathogenic nematodes, insect pathogenic nematodes and other organisms and tissues. The present invention is mainly suitable for biological and life science laboratories and testing stations in agriculture, forestry, medicine, etc.
Description
Technical field
The present invention relates to a kind of tiny organism Potter-Elvehjem Tissue Grinders.
Background technology
Researcher or testing staff needed sample is carried out homogenized before biological sample is detected.Therefore tiny organism body and microcomponent's homogenate thereof are necessary processes in farming, woods, doctor and a lot of biology and the breadboard multiple research of life science.For example to qualitative and quantitative analysis of chemical substance and residues of pesticides in the mensuration of biosome or certain composition of portion of tissue, the biosome etc.Need life active compounds such as protein and DNA be extracted in biosome or portion of tissue homogenate afterwards and carried out qualitative and quantitative comparative analysis along with the increasing experiment of development of molecular biology in recent years.
Potter-Elvehjem Tissue Grinders commonly used has the broken appearance of manual glass Potter-Elvehjem Tissue Grinders, electronic tissue refiner and ultrasonic wave tissue in the laboratory at present.Though manually glass Potter-Elvehjem Tissue Grinders low price is easy to use but make it bad like the crushing effect of the small tissue of plant pathogeny line insect, entomopathogenic nematode, mite class and the biology of the phase of infecting etc. to small biology because the distance in its mallet and tube wall space is certain.Thereby the broken appearance of electronic tissue refiner and ultrasonic wave tissue is except the big shortcoming of handling in addition that amount of samples is many, the time is grown and treated sample is rapidly heated and destroy some composition in some sample of the volume that costs an arm and a leg.
Summary of the invention
Thereby the present invention for solve existing tiny organism Potter-Elvehjem Tissue Grinders exist crushing effect bad, handle many, broken times of amount of samples and grow and be prone to that treated sample is rapidly heated and destroy the problem of some composition in some sample, and then a kind of hot-melt adhesive micro-tissue homogenizer is provided.
The present invention solves the problems of the technologies described above the technical scheme of taking to be: hot-melt adhesive micro-tissue homogenizer according to the invention is made up of body 1 and the mallet body 2 that is inserted in the body 1, and said body 1 is processed by hot melt adhesive material, and said mallet body 2 is stainless steel hammer bodies.
The invention has the beneficial effects as follows:
What The present invention be directed to that the shortcoming of the existing product of mentioning in laboratory scientific research personnel's demand and the background technology develops a kind ofly is mallet with the stainless steel bar and is a kind of Potter-Elvehjem Tissue Grinders of body with the container that the hot melt adhesive material is processed; The present invention compares with existing several kinds of homogenate devices and has the following advantages:
To the degree of crushings of small biology such as nematode etc. apparently higher than other products;
2. thereby not producing any heat in the shattering process keeps original composition not to be damaged;
3. error significantly reduces between each repetition of experiment owing to fragmentation thoroughly causes;
4. production prices are cheap;
5. solid difficult fragmentation;
6. sample loss is little in the shattering process, is more suitable for the processing in micro-example;
7, make sample fully broken, to various materials such as sugar, the detected level of albumen etc. is high, more can reflect the real content of sample.
The present invention mainly is applicable to biology and Life Science Laboratory and measuring stations etc. such as farming, woods, doctor.
The present invention adopts hot melt adhesive (ethylene vinylacetate, be called for short EVA) material to make the body of homogenizer, and is that material is made mallet with the stainless steel.The top of mallet and the bottom of body are smooth semisphere.Lean on the elasticity of hot melt adhesive body between mallet and the body and keep tight contact.The structure of this homogenizer is similar with glass homogenizer with principle of work but different is that thermosol rubber-like tube wall keeps closely contacting with the surface of stainless steel hammer in the course of the work.Its crushing effect has but substantially exceeded glass homogenizer when the space of organizing fragment through this almost nil distance.Experiment gained digital proof in the identical time (minute); Adopting this homogenizer can make the phase of the infecting nematode of entomopathogenic nematode be crushed to girth is that the following fragment of 100um accounts for 80%, and the above fragment of 200um accounts for more than 80% in the sample of glass homogenizer or the fragmentation of ultrasonic treatment device.Therefore, the detected level of trehalose in institute's test sample article is improved more than 10%, the detected level of holoprotein has been improved 15-20%.Thereby the present invention is better than present like product to the sample crushing performance that is processed also makes the experimental error between various processes obtain due control.The thermosol micro-tissue homogenizer has solved the tiny organism body or has been organized in the homogenate process thoroughly and possibly receives the problem that high temperature destroys.Also in the homogenate process, save simultaneously the time and guaranteed the minimum loss amount of micro-example in the homogenate process.The thermosol micro-tissue homogenizer is made easily, is convenient to batch production production to meet the need of market.A stainless steel hammer can be equipped with a plurality of hot melt adhesive bodys simultaneously, even can be disposable with being packaged into after the body sterilization.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present invention, and Fig. 2 is that the master of the body of hot melt adhesive material looks cut-open view, and Fig. 3 is that the master of the mallet of stainless steel looks cut-open view.
Embodiment
Embodiment one: shown in Fig. 1～3, the described hot-melt adhesive micro-tissue homogenizer of this embodiment is made up of body 1 and the mallet body 2 that is inserted in the body 1, and said body 1 is processed by hot melt adhesive material, and said mallet body 2 is stainless steel hammer bodies.
The said hot melt adhesive of this embodiment is half flexible hot melt adhesive.The surface of mallet body 2 and body 1 inner surface are smooth as far as possible, and the specification of homogenizer can be by the work internal diameter φ 3 of body from 3.0 to 10.0mm bi-directional scalings.When selecting the specification of homogenizer by to handle sample volume select.Thereby the body of being processed by hot melt adhesive material 1 and stainless steel hammer body 2 combine and use realized mallet body 2 outside surfaces and body 1 inwall just tight to contact to make be broken tiny organism body or tissue to reach best crushing effect.Thereby the slit between body 1 and the stainless steel hammer body 2 is approached zero and is reached to tiny organism such as plant pathogeny line insect the homogenate effect of entomopathogenic nematode and other biological and tissue.
Body described in this embodiment 1 also can be processed by elastic materials such as plastics, rubber.
Embodiment two: as shown in Figure 2; The said body 1 of this embodiment is made up of tubular open section 1-1, spherical section 1-2 and body main paragraph 1-3 three parts; Tubular open section 1-1, spherical section 1-2 and body main paragraph 1-3 from top to bottom set gradually and the three is communicated with; Body main paragraph 1-3 is made up of last pipe 1-3-1, middle pipe 1-3-2 and half sphere shape bottom 1-3-3; Last pipe 1-3-1, middle pipe 1-3-2 and half sphere shape bottom 1-3-3 from top to bottom set gradually and the three is communicated with, and the inner chamber of last pipe 1-3-1 is the truncated cone-shaped of toe-in gradually from top to bottom, and the inner chamber of middle pipe 1-3-2 is cylindrical; The inner chamber of the inner chamber of last pipe 1-3-1 and middle pipe 1-3-2 seamlessly transits, and the negative camber radius of half sphere shape bottom 1-3-3 is 1/2nd of a middle pipe 1-3-2 internal diameter.Other composition and annexation are identical with embodiment one.
Embodiment three: shown in Fig. 1～3, the active section diameter phi of the said mallet body 2 of this embodiment equates with the work internal diameter φ 3 of middle pipe 1-3-2.The working portion internal diameter of body should be consistent with the working position of mallet or be slightly smaller than the diameter of mallet, can make the homogenate better effects if like this.Other composition and annexation are identical with embodiment two.
Embodiment four: as shown in Figure 3, the profile of the said mallet body 2 of this embodiment is the T font.Other composition and annexation are identical with embodiment one, two or three.
Embodiment five: shown in Fig. 1～3; The internal diameter φ 1 of the tubular open section 1-1 of the described body 1 of this embodiment is 5.0mm; The internal diameter φ 2 at the open upper end place of last pipe 1-3-1 is 4.0mm; The work internal diameter φ 3 of middle pipe 1-3-2 is 3.5mm, and the external diameter φ 4 of body main paragraph 1-3 is 7.5mm; The height L1 of tubular open section 1-1 is 5.0mm, and the height L2 of spherical section 1-2 is 20mm, and the height L3 of last pipe 1-3-1 is 15mm, and the overall height L4 of middle pipe 1-3-2 and half sphere shape bottom 1-3-3 is 25mm; The height H of mallet body 2 is 100mm, and the working end diameter phi of mallet body 2 is 3.5mm, and the external diameter φ 5 of the handle of mallet body 2 is 3.5mm.Other composition and annexation are identical with embodiment one.
Above-mentioned embodiment should not be regarded as limitation of the present invention, but any improvement of doing based on design of the present invention, all should be within protection scope of the present invention.
The process of utilizing the present invention to carry out homogenized is:
Earlier sample aqueous solution is added the body inner bottom part when sample is carried out homogenate and must not surpass the 25mm height.Then mallet is slowly inserted body and moved up and down homogenate 5 minutes.After the homogenate mallet is slowly taken out cleaning, with thin glass or plastic suction pipe sample in the body is washed out then.
Claims (4)
1. hot-melt adhesive micro-tissue homogenizer, said Potter-Elvehjem Tissue Grinders is made up of body (1) and the mallet body (2) that is inserted in the body (1), and it is characterized in that: said body (1) is processed by hot melt adhesive material, and said mallet body (2) is the stainless steel hammer body.
2. hot-melt adhesive micro-tissue homogenizer according to claim 1; It is characterized in that: said body (1) is made up of tubular open section (1-1), spherical section (1-2) and body main paragraph (1-3) three parts; Tubular open section (1-1), spherical section (1-2) and body main paragraph (1-3) from top to bottom set gradually and the three is communicated with; Body main paragraph (1-3) is made up of last pipe (1-3-1), middle pipe (1-3-2) and half sphere shape bottom (1-3-3); Last pipe (1-3-1), middle pipe (1-3-2) and half sphere shape bottom (1-3-3) from top to bottom set gradually and the three is communicated with; The inner chamber of last pipe (1-3-1) is the truncated cone-shaped of toe-in gradually from top to bottom; The inner chamber of middle pipe (1-3-2) is cylindrical, and the inner chamber of the inner chamber of last pipe (1-3-1) and middle pipe (1-3-2) seamlessly transits, and the negative camber radius of half sphere shape bottom (1-3-3) is 1/2nd of middle pipe (1-3-2) internal diameter.
3. hot-melt adhesive micro-tissue homogenizer according to claim 2 is characterized in that: the active section diameter (φ) of said mallet body (2) equates with the work internal diameter (φ 3) of middle pipe (1-3-2).
4. according to claim 1,2 or 3 described hot-melt adhesive micro-tissue homogenizers, it is characterized in that: the profile of said mallet body (2) is the T font.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101366731A CN101799383B (en) | 2010-03-31 | 2010-03-31 | Hot melt adhesive micro homogenizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101366731A CN101799383B (en) | 2010-03-31 | 2010-03-31 | Hot melt adhesive micro homogenizer |
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| Publication Number | Publication Date |
|---|---|
| CN101799383A CN101799383A (en) | 2010-08-11 |
| CN101799383B true CN101799383B (en) | 2012-04-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010101366731A Expired - Fee Related CN101799383B (en) | 2010-03-31 | 2010-03-31 | Hot melt adhesive micro homogenizer |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102220224B (en) * | 2011-05-19 | 2014-10-01 | 汪华 | Automatic medical cell separation device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4307846A (en) * | 1979-10-09 | 1981-12-29 | Spelsberg Thomas C | Continuous flow tissue homogenizer |
| US4397425A (en) * | 1980-05-19 | 1983-08-09 | Pennzoil Company | Grinding apparatus |
| US5829696A (en) * | 1997-08-27 | 1998-11-03 | Michelle S. DeStefano | Sealed grinding and homogenizing apparatus |
| CN201378127Y (en) * | 2009-04-14 | 2010-01-06 | 中国水产科学研究院东海水产研究所 | Organization ultrasonic homogenate experimental device |
-
2010
- 2010-03-31 CN CN2010101366731A patent/CN101799383B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4307846A (en) * | 1979-10-09 | 1981-12-29 | Spelsberg Thomas C | Continuous flow tissue homogenizer |
| US4397425A (en) * | 1980-05-19 | 1983-08-09 | Pennzoil Company | Grinding apparatus |
| US5829696A (en) * | 1997-08-27 | 1998-11-03 | Michelle S. DeStefano | Sealed grinding and homogenizing apparatus |
| CN201378127Y (en) * | 2009-04-14 | 2010-01-06 | 中国水产科学研究院东海水产研究所 | Organization ultrasonic homogenate experimental device |
Non-Patent Citations (1)
| Title |
|---|
| 冯立才.无菌匀浆器的研制和试验.《食用菌》.2007,(第01期),56-57. * |
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| CN101799383A (en) | 2010-08-11 |
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Granted publication date: 20120425 Termination date: 20130331 |