CN101774859B - Organic liquid bio-fertilizer and preparation method thereof - Google Patents
Organic liquid bio-fertilizer and preparation method thereof Download PDFInfo
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Abstract
本发明涉及利用蝇蛆和蚯蚓养殖后的残料制备一种有机液体肥料，尤其是一种有机液体生物肥料及其制备方法。该肥料由下述重量份的原料组成：蚯蚓粪6～10份、蝇蛆粪0～4份、尿素1～3份、磷酸一铵2～4份、硫酸钾2～4份、水85～95份。其制备方法步骤如下：(1)先将蚯蚓粪(含水量为37.84％)6～10份和蝇蛆粪(含水量为37.84％)0～4份混合，然后加入50份的深井水，室温下1000rm/min搅拌20min，将混合物沉淀后，上清液过滤。(2)将剩余份数的深井水重新按(1)所述步骤清洗沉淀物，合并滤液。(3)依次加入尿素2份、磷酸一铵3份、硫酸钾3份，室温下1000rm/min搅拌10min，并将该液体肥料按质量比和嗜酸乳杆菌扩培养液9∶1加入，得到有机液体肥料。The invention relates to the preparation of an organic liquid fertilizer by utilizing residues after breeding of fly maggots and earthworms, especially an organic liquid biological fertilizer and a preparation method thereof. The fertilizer is composed of the following raw materials in parts by weight: 6 to 10 parts of vermicompost, 0 to 4 parts of fly maggot droppings, 1 to 3 parts of urea, 2 to 4 parts of monoammonium phosphate, 2 to 4 parts of potassium sulfate, and 85 to 4 parts of water. 95 servings. Its preparation method steps are as follows: (1) first mix 6～10 parts of vermicompost (water content is 37.84%) and fly maggot droppings (water content is 37.84%) 0～4 parts, then add 50 parts of deep well water, room temperature Stirring at 1000 rm/min for 20 min, the mixture was precipitated, and the supernatant was filtered. (2) Clean the sediment with the remaining parts of deep well water according to the steps described in (1), and combine the filtrates. (3) Add 2 parts of urea, 3 parts of monoammonium phosphate, and 3 parts of potassium sulfate successively, stir at 1000 rm/min for 10 min at room temperature, and add the liquid fertilizer and the expansion culture solution of Lactobacillus acidophilus in a mass ratio of 9:1 to obtain Organic liquid fertilizer.
Description
Technical field
The invention belongs to biological organic fertilizer and fabricating technology field thereof, especially relate to the defective material that utilizes after fly maggot and vermiculture and prepare a kind of organic liquid fertilizer, comprise simultaneously a kind of preparation method of organic liquid bio-fertilizer.
Background technology
The fertilizer organic fertilizer that to be organic solid waste form through microbial fermentation, deodorizing and the post-treatment that becomes thoroughly decomposed fully.At present, organic solid mainly includes solid waste that machine rubbish, stalk, feces of livestock and poultry, grouts, agricultural byproducts, food-processing produces and insect ight soil etc.Organic liquid bio-fertilizer is after fermenting by organic solid, the liquid portion that obtains by certain process means.
The research overview that utilizes fly maggot excrement and wormcast to make fertilizer is such, for the fly maggot excrement, great majority research invention mainly is made into organic fertilizer or mixed organic fertilizer with the fly maggot excrement, for example: Wu Jianwei, " method of making organic fertilizer by utilizing housefly larva to ecologically treat pig dung "; Liu Chunling, " Flyblow manure compound fertilizer and production method thereof "; Xu Debin etc., " Flyblow manure compound fertilizer and production method thereof ".For wormcast, great majority research invention mainly is made into organic fertilizer with wormcast, for example: Shen star, " a kind of wormcast fertilizer preparation method based on Herba Eichhorniae "; Chen Riguang, " earthworm biological organic fertilizer and preparation method thereof ".
At present, organic liquid bio-fertilizer is studied less, and is main still with after the organic solid fermentation, formulated with some additives of interpolation or biological inoculum by certain process means.For example: Yang Xiong, " a kind of plant-sourced organic liquid fertilizer " mainly utilizes the plant-sourced organic products of protein, starch based, carbohydrate or its tankage to form for raw material carries out ferment making; Weng Yuping, " organic fertilizer ", this fertilizer adopt biotechnology and electrolysis tech to work in coordination, and make additive with microorganism and mineralized material, add that nutritional formula is made.Liu Guozhu, " biologic compound fertilizer ", mixed by 20 multiple beneficial bacteriums such as vinelandii, phosphorus bacteria, potassium bacterium, actinomycetes, the substratum that forms take raw materials such as dish cake, wormcast or chicken manure, ground phosphate rock, nutritional supplements, peat composed of rotten mosses powder and trace elements is as carrier, and is formulated.Other invention and research are mainly that fly maggot excrement and wormcast are used as SOLID ORGANIC fertilizer, and the present invention utilizes fly maggot excrement and wormcast by with phreatic water dissolving soluble components wherein, then adds in right amount inorganic components and obtain organic liquid bio-fertilizer.The method technique is simple, and is easy to operate, is easy to promotion and application.Utilize defective material after fly maggot and vermiculture to carry out deep processing and obtain the recycling of resource, by utilizing the soluble components in water dissolution fly maggot excrement and wormcast, being prepared into organic liquid bio-fertilizer, the application prospect of its liquid fertilizer is very wide.
Summary of the invention
The object of the invention is to for utilizing the low deficiency of added value after existing feces of livestock and poultry microorganism and biological treatment, take wormcast and fly maggot excrement as main raw material, provide a kind of fertilizer efficiency remarkable, easy to use, safe, and can improve the organic liquid bio-fertilizer of crop, vegetables and fruit quality, output and raising economic worth.
Realize that technical solution of the present invention is as follows: a kind of organic liquid fertilizer is characterized in that this fertilizer is comprised of the raw material of following weight parts: 6～10 parts of wormcasts (water content is 37.84%), 0～4 part, fly maggot excrement (water content is 37.84%), 1～3 part, urea, 2～4 parts of monoammonium phosphates, 2～4 parts of vitriolate of tartar, 85～95 parts, water.(the part here refers to quality, i.e. kg) according to the preparation method of described a kind of organic liquid fertilizer, is characterized in that step is as follows:
(1) first with 6～10 parts of wormcasts (water content is 37.84%) and 0～4 part of mixing of fly maggot excrement (water content is 37.84%), then the phreatic water that adds 50 parts, under room temperature, 1000rm/min stirs 20min, and after mixture precipitation, supernatant liquor 100 mesh filter screens filter.
The phreatic water that (2) will remain umber is pressed (1) described step washing and precipitating thing, merging filtrate again.
(3) add successively 2 parts, urea, 3 parts of monoammonium phosphates, 3 parts of vitriolate of tartar, under room temperature, 1000rm/min stirs the 10min liquid fertilizer, and this liquid fertilizer is expanded nutrient solution (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) with Lactobacterium acidophilum in mass ratio add at 9: 1, obtain organic liquid bio-fertilizer.
(4) with PH precision test paper bar to this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope.PH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.The optimum formula weight part is mainly wormcast: fly maggot excrement=8: 2; General is wormcast: fly maggot excrement=9: 1, or 10: 0; The highest is wormcast: fly maggot excrement=7: 3; Minimum is wormcast: fly maggot excrement=6: 4.Its method be mainly first with wormcast (water content is 37.84%) and fly maggot excrement (water content is 37.84%) by after certain formulated, with phreatic water flushing wormcast repeatedly and the mixture of fly maggot excrement, by precipitation, obtain liquid after filtration, this fertilizer that then liquid and the bacterium liquid that spreads cultivation is mixed to get.
Organic liquid bio-fertilizer of the present invention detects through Leshan Teachers College's chemistry and Life Science College, and its detected result is as follows:
One, the measurement result of toxic substance (heavy metal) is as follows:
Mercury and compound (in Hg) 0mg/L; Cadmium and compound (in Cd) 0mg/L; Chromium and compound (in Cr)≤0.54mg/L; Arsenic and compound (in As)≤0.06mg/L; Lead and compound (in Pb)≤0.02mg/L; 0/L of roundworm egg; Colititre≤4.6 * 10
-2Individual/L.
Two, the detected result of nutritive ingredient:
Free aminoacid content is 〉=10.3g/L; Micronutrient levels is 〉=2.57g/L; Organic (take C) is 〉=5.5%; Liquid nitrogen 〉=14.8g/L, phosphorus 9.31g/L, potassium 11.6g/L; Phytokinin, hestening rooting factor B TS are 〉=0.06%.
Implement beneficial effect of the present invention and be embodied in,
1, effectively utilized residue waste material after fly maggot and vermiculture, the change waste resource is renewable resource;
2, the fly maggot excrement contains abundant trace element, high nitrogen and phosphorus, and alkali-hydrolyzable nitrogen content is higher by 125.8% than general matrix, and available phosphorus content is higher by 201.9% than general matrix
[2]Wormcast contains abundant nutrient, effective constituent is high, not only contains the major element that plants needs, and the more important thing is by the microbial fermentation effect, mineral element is become water miscible easily by the effective constituent of plant absorbing, and unknown plant hormone.
3, show after enforcement, the enforcement of this organic liquid bio-fertilizer can improve fruit yield, promotes the breeding of soil microorganisms, the sickness rate that reduces plant and improvement water surrounding to play vital role.
4, this organic liquid bio-fertilizer does not contain any hormone, heavy metal and artificial chemical composition of adding.The results showed, can be used as crop foliage fertilizer and base manure, can improve crop yield 6.8～35%, farm crop are reduced agricultural chemicals usage quantity 55%～80%; Can significantly accelerate the planktonic reproduction speed of water body, improve water quality, improvement water surrounding, suppress disease, improve the fishery product yield and quality, fertile bait dual-purpose, comprehensive benefit is higher.
Embodiment
Below in conjunction with embodiment and application implementation, the present invention is described in further details.
Embodiment 1:
(1) first with 6～10 parts of wormcasts (water content is 37.84%) and 0～4 part of mixing of fly maggot excrement (water content is 37.84%), then add the phreatic water of 50 parts, have ready conditions and also can use distilled water; Under room temperature, 1000rm/min stirs 20min, and after mixture precipitation, supernatant liquor 100 mesh filter screens filter.
The phreatic water that (2) will remain umber is again by (1) described step washing and precipitating thing, and merging filtrate also filters.
(3) add successively 2 parts, urea, 3 parts of monoammonium phosphates, 3 parts of vitriolate of tartar, under room temperature, 1000rm/min stirs 10min and obtains liquid fertilizer, and with this liquid fertilizer in mass ratio and Lactobacterium acidophilum (bacterial classification number: IFFI06005 China the culture presevation council) expand nutrient solution and add at 9: 1, obtain khaki organic liquid bio-fertilizer.
(4) with PH precision test paper bar to this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope.PH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.Its described Lactobacterium acidophilum is expanded the nutrient solution preparation of (be called for short and expand nutrient solution): enrichment medium, it is skimming milk 10%+ enzymolysis whey 6%, glucose 1.6%, soy peptone 0.5%, yeast powder 0.7%, Tomato juice 12%, flat mushroom juice 12%, 16%, 37 ℃ of fermentation culture 32h of beer reaches 3.5 * 10 by live bacterial count
8Cfu/ml gets final product.But Lactobacterium acidophilum is expanded the preparation source reference of nutrient solution: Li Chun, Zhang Lanwei, Liu Libo. the determining of growth promoting cultrure medium for Lactobacillus acidophillus [J]. and Guangzhou Food Industry science and technology .2004 (2): 12-13.
Embodiment 2:
Wormcast (water content is 37.84%) 0.8Kg and fly maggot excrement (water content is 37.84%) 0.2Kg are mixed, then add the phreatic water of 5Kg, room temperature 1000rm/min stirs 20min, and 100 mesh filter screens of supernatant liquor after mixture precipitation are filtered.The phreatic water of residue 5Kg is pressed front described step washing and precipitating thing, merging filtrate again.Add successively urea 0.2Kg, monoammonium phosphate 0.3Kg, vitriolate of tartar 0.3Kg, room temperature 1000rm/min stirs 10min, obtain liquid fertilizer, and Lactobacterium acidophilum (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) is expanded nutrient solution 0.11Kg add, obtain khaki organic liquid bio-fertilizer; To this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope with PH precision test paper bar.Obtaining pH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.
Embodiment 3:
Wormcast (water content is 37.84%) 0.9Kg and fly maggot excrement (water content is 37.84%) 0.1Kg are mixed, then add the phreatic water of 5Kg, room temperature 1000rm/min stirs 20min, and 100 mesh filter screens of supernatant liquor after mixture precipitation are filtered.The phreatic water of residue 5Kg is pressed front described step washing and precipitating thing, merging filtrate again.Add successively urea 0.2Kg, monoammonium phosphate 0.3Kg, vitriolate of tartar 0.3Kg, room temperature 1000rm/min stirs 10min, obtain liquid fertilizer, and Lactobacterium acidophilum (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) is expanded nutrient solution 0.11Kg add, obtain khaki organic liquid bio-fertilizer; To this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope with PH precision test paper bar.Obtaining pH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.
Embodiment 4:
With wormcast (water content is 37.84%) 1Kg, add the phreatic water of 5Kg, room temperature 1000rm/min stirs 20min, and 100 mesh filter screens of supernatant liquor after mixture precipitation are filtered.The phreatic water of residue 5Kg is pressed front described step washing and precipitating thing, merging filtrate again.Add successively urea 0.2Kg, monoammonium phosphate 0.3Kg, vitriolate of tartar 0.3Kg, room temperature 1000rm/min stirs 10min, obtain liquid fertilizer, and Lactobacterium acidophilum (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) is expanded nutrient solution 0.11Kg add, obtain khaki organic liquid bio-fertilizer; To this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope with PH precision test paper bar.Obtaining pH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.
Embodiment 5:
Wormcast (water content is 37.84%) 0.7Kg and fly maggot excrement (water content is 37.84%) 0.3Kg are mixed, then add the phreatic water of 5Kg, room temperature 1000rm/min stirs 20min, and 100 mesh filter screens of supernatant liquor after mixture precipitation are filtered.The phreatic water of residue 5Kg is pressed front described step washing and precipitating thing, merging filtrate again.Add successively urea 0.2Kg, monoammonium phosphate 0.3Kg, vitriolate of tartar 0.3Kg, room temperature 1000rm/min stirs 10min, obtain liquid fertilizer, and Lactobacterium acidophilum (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) is expanded nutrient solution 0.11Kg add, obtain khaki organic liquid bio-fertilizer, to this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope with PH precision test paper bar.Obtaining pH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.
Embodiment 6:
Wormcast (water content is 37.84%) 6Kg and fly maggot excrement (water content is 37.84%) 4Kg are mixed, then add the phreatic water of 50Kg, room temperature 1000rm/min stirs 20min, and 100 mesh filter screens of supernatant liquor after mixture precipitation are filtered.The phreatic water of residue 50Kg is pressed front described step washing and precipitating thing, merging filtrate again.Add successively urea 2Kg, monoammonium phosphate 3Kg, vitriolate of tartar 3Kg, room temperature 1000rm/min stirs 10min, obtain liquid fertilizer, and Lactobacterium acidophilum (bacterial classification number: the Chinese culture presevation of IFFI 06005 council) is expanded nutrient solution 1.1Kg add, obtain khaki organic liquid bio-fertilizer; To this liquid fertilizer pH value of testing, sampling is carried out the check of living bacteria count with biomicroscope with PH precision test paper bar.Obtaining pH value is 4～5, and living bacteria count is 1 * 10
5～2 * 10
6Be qualified organic liquid bio-fertilizer.
Using method: during as vegetables or fruit foliage fertilizer, this fertilizer 100-200 is doubly diluted spray, or use 50-100Kg/hm
2
Applied research embodiment on vegetable crop:
Test unit: Leshan Teachers College's chemistry and Life Science College
Trial crops: cucumber, tomato
Test site: peace village, car Zhenping, Shizhong District, Leshan
Test design and method: test is divided into 3 processing, and (CK) do not apply fertilizer (1); (2) organic and inorganic fertilizer;
(3) liquid bioorganic fertilizer.Each processes 3 repetitions.Liquid bioorganic fertilizer is pressed 100kg/667m
2, make base manure and apply for 1 time.
Vitamins C (Vc) is measured with 2,6 one dichlorophenol indophenol sodiums (2,6 one D) volumetry; Soluble sugar is measured with fearing the ketone colorimetric method for determining; Titratable acid is measured and is used the standard acid base titration.
The impact (field comparison test) of table 1 liquid bioorganic fertilizer on vegetable crop
The impact (field comparison test) of table 2 liquid bioorganic fertilizer on quality of vegetable
The testing data of field comparison test shows: the total Soluble Sugar of cucumber, Vc, and the present invention and control group increase more respectively 26.7%, 68.2%, and titratable acid descends 37.1%; The total Soluble Sugar of tomato, Vc, the present invention and control group increase more respectively 47.8%, 33.7%, and titratable acid descends 15.3%;
Applied research embodiment on fruit:
Test unit: Leshan Teachers College's chemistry and Life Science College
Trial crops: pears, Pon mandarin orange, grape
Test site: car town, Shizhong District, Leshan and taking food as usual town
Test design and method: test is divided into 2 processing, (1) control group (CK), spray clear water; (2) test group
If spray biological organic liquid fertilizer (foliage fertilizer use).Liquid bioorganic fertilizer is pressed 100kg/667m
2, spray in fruit expanding period, every 10d1 time, even spray 5 times and apply.Detect vitamins C (Vc), soluble sugar and titratable acid after fruit harvesting.Vitamins C (Vc) is measured with 2,6 one dichlorophenol indophenol sodiums (2,6 one D) volumetry; Soluble sugar is measured with fearing the ketone colorimetric method for determining; Titratable acid is measured and is used the standard acid base titration.
The impact of the biological organic liquid fertilizer of table 6 on output and fruit quality
Testing data shows: the unit output of pears, Pon mandarin orange, grape improves respectively 13.7%, 5.9%, 7.5%; Its vitamins C (Vc), soluble sugar improve respectively 10.0%, 11.5%, 3.8%; Titratable acid reduces respectively 14.8%, 8.68%, 25.1%.
Applied research embodiment in aquaculture:
Test unit: Leshan Teachers College's chemistry and Life Science College
Trial crops: grass carp, silver carp
Test site: fish farm, Shuan Shui village, taking food as usual town, Shizhong District, Leshan
Test design and method: test is divided into 2 processing, (1) inorganic fertilizer (CK); (2) organic liquid bio-fertilizer.2000kg/667m
2Chicken manure is made base fertilizer, puts fry after cultivation water quality, and fishpond and the fry situation of putting in a suitable place to breed see Table 3.Common fertilizer is used and is 667m
2Each 5kg urea+10kg carbon ammonium+10kg calcium superphosphate, 3 times per month; Biological organic fertilizer 667m
2Use 25kg, 3 times per month at every turn.During fry growth, every first quarter moon is measured the index such as planktonic growth population in dissolved oxygen, nitrite, ammonia-nitrogen content, pH value and the water body of each treating pond.
Fry is put situation in a suitable place to breed in table 3 different treatment fishpond
Table 4 different treatment is on pond water quality and planktonic impact research
The impact of table 5 different treatment on sashimi (raw fish) length and output
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| CN109400373A (en) * | 2018-12-06 | 2019-03-01 | 天津市琪梦环保科技有限公司 | A kind of slow-release fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103011909B (en) * | 2013-01-07 | 2016-06-22 | 北京清大元农生物科技有限公司 | Production method based on the Liquid Fertilizer matrix solution of wormcast |
| CN103936492A (en) * | 2014-05-15 | 2014-07-23 | 王和绥 | Plant nutrient solution |
| CN105000938A (en) * | 2015-08-04 | 2015-10-28 | 成都市四友化学工业有限责任公司 | Bio-organic fertilizer used for improving saline soil |
| CN105061039A (en) * | 2015-08-04 | 2015-11-18 | 成都市四友化学工业有限责任公司 | Preparation method of bioorganic fertilizer for improving saline soil |
| CN105367229A (en) * | 2015-11-17 | 2016-03-02 | 张山虎 | Liquid microbial ecological fertilizer and preparation method thereof |
| CN105272661A (en) * | 2015-11-26 | 2016-01-27 | 天津天隆农业科技有限公司 | Method for preparing leaf fertilizer from lumbrokinase |
| CN108117435A (en) * | 2016-11-29 | 2018-06-05 | 张宝金 | A kind of Chromium-rich nutrition solution and its preparation method and application |
| CN107129328A (en) * | 2017-05-24 | 2017-09-05 | 钟志雄 | A kind of preparation method of high calcium earthworm organic foliar fertilizer |
| CN119344218B (en) * | 2024-12-12 | 2025-07-18 | 乐山师范学院 | Method for precisely cultivating populus tomentosa tissue |
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| CN1091243A (en) * | 1993-02-26 | 1994-08-31 | 河北省唐县生物工程公司 | A kind of growth regulating agent for animal and plant and preparation method thereof |
| CN1385384A (en) * | 2002-06-18 | 2002-12-18 | 潘金环 | Methd for industrilized organic refuse treatment and fly maggot and worm lizard cultivation |
| CN1994074A (en) * | 2006-12-19 | 2007-07-11 | 邱云生 | An orange garden-pest-chicken biological chain breeding method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1091243A (en) * | 1993-02-26 | 1994-08-31 | 河北省唐县生物工程公司 | A kind of growth regulating agent for animal and plant and preparation method thereof |
| CN1385384A (en) * | 2002-06-18 | 2002-12-18 | 潘金环 | Methd for industrilized organic refuse treatment and fly maggot and worm lizard cultivation |
| CN1994074A (en) * | 2006-12-19 | 2007-07-11 | 邱云生 | An orange garden-pest-chicken biological chain breeding method |
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| CN109400373A (en) * | 2018-12-06 | 2019-03-01 | 天津市琪梦环保科技有限公司 | A kind of slow-release fertilizer |
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