CN101773149A - Bacillus subtilis Bs-03 wettable powder preparation and water-dispersing granule preparation - Google Patents
Bacillus subtilis Bs-03 wettable powder preparation and water-dispersing granule preparation Download PDFInfo
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Abstract
The invention discloses a wettable powder preparation and a water-dispersing granule preparation, which use bacillus subtilis Bs-03 as active ingredients. The bacillus subtilis Bs-03 water-dispersing granule preparation is prepared from bacillus subtilis Bs-03 raw powder, precipitated calcium carbonate, glucose, wetting agents 1004, fish meal and CMC. The bacillus subtilis Bs-03 wettable powder preparation is prepared from bacillus subtilis Bs-03 raw powder, white carbon black, glucose, starch, wetting agents 1004, dispersing agents NNO and CMC. The bacillus subtilis Bs-03 wettable powder preparation and the water-dispersing granule preparation of the invention have stable property, are convenient to store for a long time, and have the obviously prevention and treatment effect on the cotton wilt disease, and the prevention and treatment effect is higher than 60 percent.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent, especially relating to a kind of is the wetting powder and the water dispersible granules of active component with bacillus subtilis (Bacillussubtilis) Bs-03.
Background technology
Formulation is one of key technology during agricultural chemicals is used, and not only because of easy to use and raising drug effect, and is convenient to standardized production and management.But the biopesticide especially dosage form selection of live body preparation must be determined through two-way; the one, must obtain stable physical behavior and standardized production basic model; and must on preparation thallus survival and control efficiency, certain hour be arranged, otherwise just can not carry out large-scale production.To this, the definite of formulation must design and test, to obtain the stable prod of certain preservation time and drug effect.Because the particularity of microorganism live body preparation, that can select at present has wetting powder, wettable tablet, water dispersible granules (WDG or WG), dried seed dressing, granule, fine granule, little suspension wafer, granula subtilis, suspending agent, 10 kinds of formulations of dry suspending agent.
The applicant is at patent " bacillus subtilis and application thereof " (number of patent application: 200910018092.5, the applying date: disclose the new bacillus subtilis strain Bs-03 of a strain 2009.8.25), it adopts the soil isolation by dilution method to separate from Shandong Forest Science Academy's experiment nursery soil and acquires, be preserved in Chinese microbial preservation common micro-organisms center (the abbreviation CGMCC of administration committee on 04 23rd, 2009, address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preserving number is CGMCC No.3038.This bacterial strain can be used as bio-fertilizer and biopesticide, and proves that by experiment this bacterial strain fermentation liquor control cotton verticillium wilt effect is remarkable, and control efficiency reaches 71.11%, and the cotton growth stalwartness, and no poisoning produces.Simple bacillus subtilis Bs-03 zymotic fluid stability is bad, and the bacterium amount of its survival reduces rapidly as time passes, therefore can not place for a long time.
Summary of the invention
The objective of the invention is to overcome the shortcoming that bacillus subtilis Bs-03 zymotic fluid can not be placed for a long time, characteristics according to bacillus subtilis Bs-03 are composed with the control to pathogen, determine to prevent and treat soil-borne disease and hinge portion disease, moisture maintains 6-8% and is advisable, and a kind of bacillus subtilis Bs-03 wetting powder and water dispersible granules of good stability are provided.
Technical scheme of the present invention is: a kind of bacillus subtilis Bs-03 water dispersible granules (WG), it is characterized in that, raw material by following weight parts is made, the former powder 1-30 of bacillus subtilis Bs-03, precipitated calcium carbonate 1-60, glucose 0.5-60, wetting agent 1004 0.5-30, fish meal 0.2-30, CMC (sodium carboxymethylcellulose) 0.02-10, its bacillus subtilis Bs-03 content are 0.1-100 hundred million viable bacteria/grams.
The preparation method: above-mentioned raw materials is mixed, and 0-50 ℃ of control processing temperature is in airslide disintegrating mill or other high order cracker; be crushed to more than 150 sieve meshes; carry out fluidized granulating or extruder grain again, moisture is controlled at 6-8% (quality percentage composition), and pH is controlled at 7-7.5.
Another kind of technical scheme of the present invention is: a kind of bacillus subtilis Bs-03 wetting powder (WP), it is characterized in that, raw material by following weight parts is made, the former powder 1-30 of bacillus subtilis Bs-03, white carbon 1-60, glucose 0.5-60, starch 0.5-30, wetting agent 1004 0.5-30, dispersant NNO0.5-20, CMC (sodium carboxymethylcellulose) 0.02-10, its bacillus subtilis Bs-03 content are 0.1-100 hundred million viable bacteria/grams.
Manufacture method: above-mentioned raw materials is mixed, and 0-50 ℃ of control processing temperature in airslide disintegrating mill or other high order cracker, is crushed to the above moisture of 150 sieve meshes and is controlled at 6-8% (quality percentage composition), and pH is controlled at 7-7.5.
The former powder of bacillus subtilis Bs-03 of the present invention is that the bacillus subtilis Bs-03 content of bacillus subtilis Bs-03 zymotic fluid after spray-dried is the dry powder of 80-300 hundred million viable bacteria/grams, and its concrete fermentation process is seen patent 200910018092.5.Described wetting agent 1004 is produced by Zouping, Shandong Dexing Fine Chemical Co., Ltd.
Using method: can use bacillus subtilis Bs-03 water dispersible granules or wetting powder 500-600 times foliar spray, or contain volume preparation 0.2% (preparation of the soil of 99.8 weight portions and 0.2 weight portion) seed dressing with this, also can convert water 1500-2000 and doubly irritate root.
Bacillus subtilis Bs-03 wetting powder and water dispersible granules different moisture content see Table 1 and table 2 to the effect of vigor of placing 0,10,30,180,360 day bacillus subtilis Bs-03 respectively.
Table 1 wetting powder is at the effect of vigor of different moisture content to bacillus subtilis Bs-03
| Moisture (%) | 0 day | 10 days | 30 days | 180 days | 360 days |
| ??4 | ??22.5 | ??21.7(96.5) | ??20.7(91.8) | ??11.9(52.7) | ??4.5(20.1) |
| ??6 | ??27.4 | ??26.4(96.2) | ??26.1(95.1) | ??20.7(75.3) | ??18.7(68.0) |
| ??8 | ??23.7 | ??23.3(98.5) | ??21.6(91.4) | ??19.9(84.0) | ??16.9(71.3) |
| ??10 | ??26.1 | ??23.1(88.8) | ??17.6(67.8) | ??12.7(68.8) | ??5.2(20.2) |
| ??12 | ??27.8 | ??21.7(77.9) | ??16.9(60.5) | ??7.9(28.3) | ??1.6(5.9) |
Annotate: numeral is three mensuration mean values in the table, and unit is 10cfu/g, is thalline survival percentage in the bracket.
Table 2 water dispersible granules is at the effect of vigor of different moisture content to bacillus subtilis Bs-03
| Moisture (%) | 0 day | 10 days | 30 days | 180 days | 360 days |
| ??4 | ??22.9 | ??22.1(96.7) | ??20.9(91.4) | ??12.7(55.4) | ??5.2(22.9) |
| ??6 | ??24.8 | ??24.5(98.7) | ??23.7(95.3) | ??19.7(79.4) | ??17.7(71.2) |
| ??8 | ??24.5 | ??23.9(97.4) | ??22.9(93.3) | ??21.2(86.6) | ??18.9(77.0) |
| ??10 | ??23.5 | ??22.4(95.6) | ??18.0(76.8) | ??15.3(65.1) | ??11.9(50.6) |
| ??12 | ??64.2 | ??21.3(82.9) | ??17.7(68.6) | ??10.1(39.2) | ??3.7(14.5) |
Annotate: numeral is three mensuration mean values in the table, and unit is 10cfu/g, is thalline survival percentage in the bracket.
From table 1 and table 2 as can be seen, vigor is better bacillus subtilis Bs-03 wetting powder and water dispersible granules are placed a period of time when moisture is controlled at 6-8% after, and the thalline survival rate is greater than 68%.
Two kinds of preparations are divided into 3 parts separately, pack with polyethylene plastic film, seal, place under the shady and cool condition of nature, avoid the sunlight direct projection, place 2 years sampling observations result and the former powder similarity conditions of bacillus subtilis Bs-03 down the sampling observation in 2 years of placement as a result correction data see Table 3, table 4 and table 5:
Table 3 20 * 10
8Cfu/g bacillus subtilis Bs-03 wetting powder product testing result
| Time | Viable count (10 8cfu/g) | Suspensibility % | Fineness | Moistening time s | Moisture % | ??PH |
| ??07.12.5 | ??22.5 | ??91.4 | ??98 | ?3.2 | ??7.3 | ??7.3 |
| ??08.6.6 | ??16.7 | ??90.2 | ??97 | ?3.6 | ??7.3 | ??7.2 |
| ??08.12.6 | ??15.2 | ??87.5 | ??96 | ?3.5 | ??7.2 | ??6.7 |
| ??09.6.6 | ??11.9 | ??86.7 | ??95 | ?4.5 | ??7.1 | ??6.5 |
| ??09.12.6 | ??8.8 | ??85.4 | ??95 | ?4.5 | ??6.9 | ??6.5 |
Annotate: sample size is 2.76 tons, and fineness is for passing through 250 sieve mesh particle percentages.
Table 4 20 * 10
8Cfu/g bacillus subtilis Bs-03 water dispersible granules product testing result
| Time | Viable count (10 8cfu/g) | Suspensibility % | Moistening time s | Moisture % | ??PH |
| ??07.12.5 | ??21.1 | ??90.8 | ?3.5 | ??7.1 | ??7.5 |
| ??08.6.6 | ??19.7 | ??88.6 | ?3.8 | ??6.9 | ??7.4 |
| ??08.12.6 | ??16.9 | ??86.4 | ?3.9 | ??6.9 | ??6.9 |
| ??09.6.6 | ??12.5 | ??85.5 | ?4.6 | ??6.8 | ??6.6 |
| ??09.12.6 | ??9.7 | ??85.1 | ?4.7 | ??6.8 | ??6.5 |
Annotate: sample size is 1.92 tons.
Former meal component changes under the same storage condition of table 5
| Time | ??07.12.5 | ??08.6.6 | ??08.12.6 | ??09.6.6 | ??09.12.6 |
| Viable count (10 8cfu/g) | ??196.7 | ??109.6 | ??49.8 | ??21.4 | ??4.2 |
| Moisture % | ??7.7 | ??7.6 | ??7.4 | ??7.4 | ??7.3 |
| ??PH | ??7.3 | ??7.3 | ??7.2 | ??7.2 | ??7.1 |
Annotate: sample size is 1.23 tons.
From table 3, table 4 and table 5 as can be seen, former powder is without processing, and the bacterium of its survival amount reduces rapidly as time passes, has only initial 2.1% after 2 years, and wetting powder and water dispersible granules are respectively 39.1% and 46.0%, prove absolutely the importance of preparation processing.
The invention has the beneficial effects as follows: bacillus subtilis Bs-03 wetting powder of the present invention and water dispersible granules stable in properties, be convenient to long-term placement, and remarkable to the cotton wilt control efficiency, control efficiency is greater than 60%.
For clear and definite bacillus subtilis Bs-03 wetting powder and the water dispersible granules control efficiency to cotton wilt, applying for it provides foundation, and we have carried out pot experiment:
1 for examination material and method
1.1 reagent agent
WP0 is a bacillus subtilis Bs-03 wetting powder (clump count 20.9 * 10
8Cfu/g), WP1 is that WP0 stores the product (clump count 15.2 * 10 after 1 year
8Cfu/g), WG0 is a bacillus subtilis Bs-03 water dispersible granules (clump count 20.4 * 10
8Cfu/g), WG1 is that WG0 stores the product (clump count 16.9 * 10 after 1 year
8Cfu/g).
1.2 for studying thing and controlling object: for studying thing is cotton, and kind is a middle cotton 17; Controlling object: fusarium wilt
1.3 test method:
1.3.1 germ and sick soil preparation: with the 121 ℃ of sterilizations 30 minutes in triangular flask of middle cotton 17 lint cotton seeds, insert the mycelia piece of fusarium wilt germ when reducing to room temperature, 28 ℃ cultivate 7 days plentiful to mycelia, grind after air-dry, the particle of getting between the 20-40 order is used for inoculation.Bacterium soil is prepared: the wilt powder that grinds after will be above-mentioned air-dry, to mix in soil, and volume is native heavy by 0.2%, rewaters to relative moisture 70-80% to sow.
1.3.2 healthy cotton seed is handled: earlier cotton seed was steeped 20 minutes with 55 ℃ of water loggings, continue at normal temperatures then to soak 24 hours, pull and remove excessive moisture out, 30 ℃ of left and right sides vernalization.
1.3.3 when seed shows money or valuables one carries unintentionally by grain weight 2% with above-mentioned medicament (the above-mentioned preparation that contains bacillus subtilis Bs-03) seed dressing, dry a little, just be sowed in the basin, 5 in every basin repeats 5 times, contrasts to be modified starch and peat composed of rotten mosses dressing.
1.4 investigation and record method
When cotton grows to 3-4 sheet true leaf, investigate.According to the withered number of cotton seedling, calculate control efficiency.
Table 6 bacillus subtilis Bs-03 wetting powder and water dispersible granules are to the cotton wilt control efficiency
| ??WP0 | ??WP1 | ??WG0 | ??WG1 | Contrast | |
| Test strain number | ??25 | ??25 | ??25 | ??25 | ??25 |
| Withered strain number | ??7 | ??7 | ??8 | ??9 | ??23 |
| Death rate % | ??28 | ??28 | ??32 | ??36 | ??92 |
| Control efficiency % | ??69.6 | ??69.6 | ??65.2 | ??60.9 | ??\ |
As can be seen from Table 6, bacillus subtilis Bs-03 wetting powder and water dispersible granules are remarkable to the cotton wilt control efficiency, and control efficiency is greater than 60%.
Embodiment
Embodiment 1: bacillus subtilis Bs-03 water dispersible granules
Prescription: the former powder 20 of bacillus subtilis Bs-03, precipitated calcium carbonate 15, glucose 55, wetting agent 6, fish meal 10, CMC 1, and its bacillus subtilis Bs-03 content is 4,500,000,000 viable bacteria/grams.
The preparation method: above-mentioned raw materials is mixed, and 0-50 ℃ of control processing temperature is at airslide disintegrating mill or other high order cracker; be crushed to more than 150 sieve meshes; carry out fluidized granulating or extruder grain again, moisture is controlled at 6-8% (quality percentage composition), and pH is controlled at 7-7.5.
Embodiment 2: bacillus subtilis Bs-03 water dispersible granules
Prescription: the former powder 20 of bacillus subtilis Bs-03, precipitated calcium carbonate 9, glucose 55, wetting agent 5, fish meal 10, CMC 1, and its bacillus subtilis Bs-03 content is 4,000,000,000 viable bacteria/grams.
The preparation method is with embodiment 1.
Embodiment 3: bacillus subtilis Bs-03 water dispersible granules
Prescription: the former powder 30 of bacillus subtilis Bs-03, precipitated calcium carbonate 5, glucose 50, wetting agent 6, fish meal 10, CMC 1, and its bacillus subtilis Bs-03 content is 8,800,000,000 viable bacteria/grams.
The preparation method is with embodiment 1.
Embodiment 4: bacillus subtilis Bs-03 wetting powder
Prescription: the former powder 20 of bacillus subtilis Bs-03, white carbon 20, glucose 41, starch 20, wetting agent 10045, dispersant NNO 2, CMC 2, and its bacillus subtilis Bs-03 content is 4,000,000,000 viable bacteria/grams.
Manufacture method: above-mentioned raw materials is mixed, and 0-50 ℃ of control processing temperature at airslide disintegrating mill or other high order cracker, is crushed to the above moisture of 150 sieve meshes and is controlled at 6-8% (quality percentage composition), and pH is controlled at 7-7.5.
Embodiment 5: bacillus subtilis Bs-03 wetting powder
Prescription: the former powder 10 of bacillus subtilis Bs-03, white carbon 35, glucose 41, starch 10, wetting agent 10045, dispersant NNO 2, CMC 2, and its bacillus subtilis Bs-03 content is 2,000,000,000 viable bacteria/grams.
The preparation method is with embodiment 4.
Embodiment 6: bacillus subtilis Bs-03 wetting powder
Prescription: the former powder 30 of bacillus subtilis Bs-03, white carbon 15, glucose 36, starch 10, wetting agent 10045, dispersant NNO 2, CMC 2, and its bacillus subtilis Bs-03 content is 6,000,000,000 viable bacteria/grams.
The preparation method is with embodiment 4.
Claims (6)
1. bacillus subtilis Bs-03 water dispersible granules, it is characterized in that, raw material by following weight parts is made, the former powder 1-30 of bacillus subtilis Bs-03, precipitated calcium carbonate 1-60, glucose 0.5-60, wetting agent 10040.5-30, fish meal 0.2-30, CMC 0.02-10, its bacillus subtilis Bs-03 content are 0.1-100 hundred million viable bacteria/grams.
2. a kind of bacillus subtilis Bs-03 water dispersible granules as claimed in claim 1 is characterized in that, bacillus subtilis Bs-03 content is 80-300 hundred million viable bacteria/grams in the former powder of described bacillus subtilis Bs-03.
3. a kind of bacillus subtilis Bs-03 water dispersible granules as claimed in claim 1 or 2 is characterized in that, described bacillus subtilis Bs-03 water dispersible granules moisture quality percentage composition is 6-8%, and pH is 7-7.5.
4. bacillus subtilis Bs-03 wetting powder, it is characterized in that, raw material by following weight parts is made, the former powder 1-30 of bacillus subtilis Bs-03, white carbon 1-60, glucose 0.5-60, starch 0.5-30, wetting agent 10040.5-30, dispersant NNO 0.5-20, CMC 0.02-10, its bacillus subtilis Bs-03 content are 0.1-100 hundred million viable bacteria/grams.
5. a kind of bacillus subtilis Bs-03 wetting powder as claimed in claim 4 is characterized in that, bacillus subtilis Bs-03 content is 80-300 hundred million viable bacteria/grams in the former powder of described bacillus subtilis Bs-03.
6. as claim 4 or 5 described a kind of bacillus subtilis Bs-03 wetting powders, it is characterized in that described bacillus subtilis Bs-03 wetting powder moisture quality percentage composition is 6-8%, pH is 7-7.5.
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| CN102007903A (en) * | 2010-10-29 | 2011-04-13 | 中国林业科学研究院森林生态环境与保护研究所 | Bacillus thuringiensis virus water dispersible granules and preparation method thereof |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
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| CN102007903A (en) * | 2010-10-29 | 2011-04-13 | 中国林业科学研究院森林生态环境与保护研究所 | Bacillus thuringiensis virus water dispersible granules and preparation method thereof |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN102894012A (en) * | 2012-05-29 | 2013-01-30 | 中国林业科学研究院森林生态环境与保护研究所 | Paecilomyces lilacinus wettable powder, preparation method and application thereof |
| CN102805107A (en) * | 2012-05-30 | 2012-12-05 | 山东京青农业科技有限公司 | Microbiological preparation for controlling apple physalospora piricola and application thereof |
| CN102805107B (en) * | 2012-05-30 | 2016-12-14 | 山东京青农业科技有限公司 | A kind of microorganism formulation preventing and treating Fructus Mali pumilae wheel stricture of vagina canker and application |
| CN102919272A (en) * | 2012-11-07 | 2013-02-13 | 湖北省生物农药工程研究中心 | Water dispersible granule for bacillus subtilis and method for preparing same |
| CN103355290A (en) * | 2013-07-18 | 2013-10-23 | 江苏省农业科学院 | Bacillus subtilis dry suspension agent and preparation method thereof |
| CN103355290B (en) * | 2013-07-18 | 2014-12-24 | 江苏省农业科学院 | Bacillus subtilis dry suspension agent and preparation method thereof |
| CN113913324A (en) * | 2021-09-01 | 2022-01-11 | 郑州轻工业大学 | Composite microbial inoculum for efficient deodorization of biological filter and preparation method thereof |
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