CN101762628A - Dry enzyme method biochip/test strip - Google Patents
Dry enzyme method biochip/test strip Download PDFInfo
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- CN101762628A CN101762628A CN200910234106A CN200910234106A CN101762628A CN 101762628 A CN101762628 A CN 101762628A CN 200910234106 A CN200910234106 A CN 200910234106A CN 200910234106 A CN200910234106 A CN 200910234106A CN 101762628 A CN101762628 A CN 101762628A
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a biological sensing chip, which is applicable to the fast diction of various indexes in the blood, including the indexes of cholesterin, triolein, blood sugar protein, blood sugar, lactic acid, uric acid, blood pH, blood oxygen and the like. The invention particularly relates to a dry enzyme method biochip/test strip, which belongs to the technical field of fast detection of a medical miniature biosensor. The dry enzyme method biochip comprises a base sheet, a dry enzyme work electrode, a reference electrode, a test liquid diffusing reticular film and hydrophobic insulation paint, wherein the dry enzyme work electrode and the reference electrode are 10 nm to 30 mum higher than the base sheet, the test liquid diffusing reticular film has the hydrophilicity, the contact angle of the test liquid diffusing reticular film and the pure water is approximately zero degree, and the test liquid including whole blood, blood serum, blood plasma or other tested liquid can be uniformly permeated and diffused onto the dry enzyme work electrode through the reticular film, so various blood parameters or other organic and inorganic liquid parameters can be tested by an electrochemical method.
Description
Technical field
The present invention relates to a kind of bio-sensing chip, be applicable to the many index in the fast detecting blood, comprising: cholesterol, three oily sweet esters, blood gp, blood sugar, lactic acid, uric acid, blood pH, indexs such as blood oxygen are equally applicable to other various instruments, as at biology, chemistry, food, fields such as environmental protection quantitatively, the qualitative analysis instrument is specifically related to a kind of dry enzyme method biochip/test strip, belongs to medical miniature biology sensor rapid detection technical field.
Background technology
The new high-tech industry that miniature quick medical analysis instrument is a rising in recent years, its core technology are molecule and biomolecule sensing technology.Biology sensor be with biologically active unit (as enzyme, antibody, nucleic acid, cell etc.) as the bio-sensing unit, target is surveyed the detecting device that thing has high selectivity.And all blood glucose meter test pieces in the market all are that its end advances blood, all adopt suction sheet or absorbing membrane (hydrophilic film/sheet) to finish the design of siphon reactive site and make.Plastics as PET, itself are hydrophobic, general suction PET plastic sheets, are through special processing, are coated with the chemical substance of last layer suction and reach PET and become the hydrophobic hydrophilic effect that is.Otherwise PET can not be used as the siphon cover plate.The another one problem is that PET is apt to deteriorate through the chemical substance (coating) of special processing suction, is damaged. under hot and humid degree, lose absorbent function very soon especially.Again it, end advances test fluid (advancing to refer to blood as blood glucose meter test pieces end), must air hole be arranged in the back end design of sample introduction, in case there is not then end sample introduction of air hole, air resistance will cause siphon to realize, not reach syphonic effect.
Summary of the invention
The objective of the invention is for overcoming above-mentioned the deficiencies in the prior art part, a kind of dry enzyme method biochip/test strip is provided, it includes substrate, be attached with dried enzyme working electrode on the substrate, contrast electrode, test fluid diffusion nethike embrane, wherein dried enzyme working electrode and contrast electrode exceed scope than substrate: 10nm-30 μ m, conductive electrode and contrast electrode thickness are 1-30 μ m during with screen printing technique.Nethike embrane possess hydrophilic property wherein, can as blood, be diffused into one or more test zones that biochip designs in advance uniformly to test fluid, and it is covered fully, carry out one (single electrode) or Several Parameters (same chip prints a plurality of biochip electrodes) test job.This dried enzyme work system is at engaged test liquid (behind blood) biologically active, and blood, is opened along netting twine nethike embrane uniform spreading by the molecular motion of self by the water wettability nethike embrane, and test fluid is able to even distribution in the dried enzyme work system of biochip zone.
The present invention realizes with following technical scheme: a kind of dry enzyme method biochip/test strip, it is characterized in that: it includes substrate, dried enzyme working electrode, contrast electrode, test fluid diffusion nethike embrane, hydrophobicity insullac is formed, and wherein dried enzyme working electrode and contrast electrode exceed scope than substrate: 10nm is to 30 μ m.
Described test fluid diffusion nethike embrane comprises whole blood with test fluid, serum, and blood plasma, or its tested liquid, thus permeating equably to be diffused into by this nethike embrane records various blood parameters and other organic and inorganic liquid parameter on the dried enzyme working electrode.
Described test fluid diffusion nethike embrane possess hydrophilic property, the contact angle of this nethike embrane and pure water equals 0 degree.
Described chip/strip places a miniature temperature-controlling chamber to test, and the test job temperature is controlled between 25 ℃-60 ℃, particularly 35 ℃-55 ℃ temperature range.
Described test fluid diffusion nethike embrane covers the dried enzyme working electrode interval that is no less than a biochip/test strip, and this dried enzyme working electrode biochip/test strip system has the enzymic catalytic reaction activity; The mensuration that can be no less than a blood parameters.
Described dried enzyme working electrode, its enzymic catalytic reaction reactive systems has biology catalytic activity, and this biology catalytic activity also comprises the non-enzymatic catalysis reaction system, enzyme system is by following but be not limited to the system that following enzyme is formed: lipoprotein lipase, glycerokinase, GPO, cholesterol esterase, cholesterin dehydrogenase, cholesterol oxidase, glucose oxidase, hemoglobinase, glucose oxidase, lactic dehydrogenase, malic dehydrogenase, bilirubin oxidase, ascorbic acid oxidase, peroxidase, uricase, clostridiopetidase A, hyaluronidase, proteinase, proteolytic enzyme, diastase, lipase, yeast extract etc., non-enzyme system class as, cellulose, starch, macromolecule and organic-inorganic thing etc.
Described contrast electrode, when carrying out the electrode catalyst reaction, each absolute electrode reaction system is formed by being no less than two electrodes.
Described electrode absolute electrode reaction system has to be no less than an absolute electrode catalyst system and catalyzing on a sensor biochip/test strip, is no less than the mensuration work of a parameter.
Described substrate, its material is PET, or other plastic basis material, or non-plastic basis material, the thickness of PET base material is between 0.05mm-2mm, conductive electrode material on the base material comprises Au, Pt, Pd, graphite, the contrast electrode material is Ag/AgCl, and the contrast electrode material also can be to carry out oxidation reaction metal organic compound, organism and inorganics.
Described test fluid diffusion nethike embrane, its material is by being made up of the PET single, or there are the PC (polycarbonate) or PS (polystyrene) film that have the cavity to form its thickness between 50-500 micron (μ m), between 100-200 micron (μ m), the size of its mesh or membrane pores is not less than 2 microns (μ m) especially.
Described dried enzyme working electrode, contrast electrode, test fluid diffuser screen, dried enzyme operation interval with biochip/test strip, its geometric configuration can be one or more shapes, comprises one of rectangle, trapezoidal, triangle, circle, semicircle, circular arc, ellipse, ellipse semicircle, elliptic arc, or its combination.
Advantage of the present invention is: the dried enzyme working electrode and the contrast electrode of this dry enzyme method biochip exceed certain thickness than substrate, do on the enzyme system and be coated with nethike embrane, nethike embrane possess hydrophilic property wherein, can be test fluid, as blood, be diffused into one or more test zones that biochip designs in advance uniformly, and it is covered fully, this dried enzyme work system is at engaged test liquid (behind blood) biologically active, blood is by the water wettability nethike embrane, by the molecular motion of self, to open along netting twine nethike embrane uniform spreading, test fluid is able to even distribution in the dried enzyme work system of biochip zone.
Description of drawings
Below in conjunction with drawings and Examples the present invention is described in further detail:
Fig. 1 is the substrate synoptic diagram:
Fig. 2 is the dried enzyme system synoptic diagram of serigraphy on electrode:
Fig. 3 is the nethike embrane synoptic diagram:
Fig. 4 is the hydrophobicity insullac synoptic diagram of printing:
Fig. 5 is that dried enzyme process nethike embrane covers biology sensor cholesterol chip/strip test findings synoptic diagram:
Among the figure: 1, substrate, 2, print electrode, 3, dried enzyme chip or strip, 4, nethike embrane, 5, be printed with the biochemical reaction zone of hydrophobicity insullac.
As shown in the figure, substrate 1 is plastics (such as PET) materials, its thickness can 0.1mm to 2mm, be attached with in the above two or more electrodes; Electrode material comprises, but is not limited to Au, Pt, Pd, Ag, Ag/AgCl composite, the materials such as graphite; The thickness of electrode can be from several nanometers (nm), (μ m) do not wait to tens microns, electrode can be a material, such as conductive carbon electrode material, Au, Pd, or Pt, also can be that two Material claddings form,, republish Ag/AgCl reference electrode material such as first printing conductive carbon electrode thereon. Dried enzyme work system 3 and by the dried enzyme work system of 5 biochip/test strips that define zone importantly, this dried enzyme work system has biologically active behind engaged test liquid (such as blood). It is manufactured and can apply by printing, and injection is shootd out, titration, or other method realizes.
Embodiment
As shown in the figure:
[1] PET: width 7mm, thickness 0.2mm; Long 37mm;
[2] be first seal China ink, width 1mm, thickness 0.005-0.015mm; Thereon, printing Ag/AgCl contrast electrode material: width 0.5mm, thickness 0.005-0.015mm wherein reserves dried enzyme electrode interval not printed silver and silver chloride compound substance, and thickness is between 5-20 μ m;
[3] be the dried enzyme system of serigraphy on carbon electrode: width 0.75-1.5mm, thickness 0.002-0.01mm;
[4] be hydrophilic nethike embrane: the nethike embrane stock is the PET fiber, fibre diameter 0.005mm, single spacing 0.15mm;
[5] be the hydrophobicity insullac of printing.
The conductive carbon pastes, Ag/AgCl reference electrode pastes and the dried enzyme system that have wherein adopted Cambridge NanoTech Limited (English sword nanosecond science and technology company) to produce.The test result of the cholesterol biochip/test strip of Zhi Zuoing as shown in Figure 5 thus: wherein the x axle is the concentration of cholesterol titer; The y axle is the magnitude of current that is produced by this electrode.The concentration of cholesterol and the electric current that records are directly proportional, and linearly dependent coefficient is up to 0.9955.
Claims (11)
1. dry enzyme method biochip/test strip, it is characterized in that: it includes substrate, dried enzyme working electrode, contrast electrode, test fluid diffusion nethike embrane, hydrophobicity insullac is formed, and wherein dried enzyme working electrode and contrast electrode exceed scope than substrate: 10nm is to 30 μ m.
2. a kind of dry enzyme method biochip/test strip according to claim 1, it is characterized in that: described test fluid diffusion nethike embrane is with test fluid, comprise whole blood, serum, blood plasma, or its tested liquid, thereby permeating equably to be diffused into by this nethike embrane records the organic and inorganic liquid parameter of various blood parameters and other on the dried enzyme working electrode.
3. a kind of dry enzyme method biochip/test strip according to claim 2 is characterized in that: described test fluid diffusion nethike embrane possess hydrophilic property, the contact angle of this nethike embrane and pure water equals 0.
4. a kind of dry enzyme method biochip/test strip according to claim 1, it is characterized in that: described chip/strip places a miniature temperature-controlling chamber to test, the test job temperature is controlled between 25 ℃-60 ℃, particularly 35 ℃-55 ℃ temperature range.
5. a kind of dry enzyme method biochip/test strip according to claim 2, it is characterized in that: described test fluid diffusion nethike embrane covers the dried enzyme operation interval that is no less than a biochip/test strip, and this dried enzyme working electrode biochip/test strip system has the enzymic catalytic reaction activity; The mensuration that can be no less than a blood parameters.
6. a kind of dry enzyme method biochip/test strip according to claim 1 is characterized in that: described dried enzyme working electrode, and its enzymic catalytic reaction reactive systems has biology catalytic activity, this biology catalytic activity also comprises the non-enzymatic catalysis reaction system, and enzyme system is by following but be not limited to the system that following enzyme is formed: lipoprotein lipase, glycerokinase, GPO, cholesterol esterase, cholesterin dehydrogenase, cholesterol oxidase, glucose oxidase, hemoglobinase, glucose oxidase, lactic dehydrogenase, malic dehydrogenase, bilirubin oxidase, ascorbic acid oxidase, peroxidase, uricase, clostridiopetidase A, hyaluronidase, proteinase, proteolytic enzyme, diastase, lipase, yeast extract etc., non-enzyme system class as, cellulose, starch, macromolecule and organic-inorganic thing etc.
7. a kind of dry enzyme method biochip/test strip according to claim 1 is characterized in that: described contrast electrode, when carrying out the electrode catalyst reaction, each absolute electrode reaction system is formed by being no less than two electrodes.
8. a kind of dry enzyme method biochip/test strip according to claim 7, it is characterized in that: described electrode absolute electrode reaction system, on a sensor biochip/test strip, have to be no less than an absolute electrode catalyst system and catalyzing, be no less than the mensuration work of a parameter.
9. a kind of dry enzyme method biochip/test strip according to claim 7 is characterized in that: described substrate, and its material is PET, or other plastic basis material, or non-plastic basis material, the thickness of PET base material is between 0.05mm-2mm, and the conductive electrode material on the base material comprises Au, Pt, Pd, graphite, the contrast electrode material is Ag/AgCl, the contrast electrode material also can be to carry out oxidation reaction metal organic compound, organism and inorganics.
10. according to claim 7-kind of dry enzyme method biochip/test strip, it is characterized in that: described test fluid diffusion nethike embrane, its material is by being made up of the PET single, or there are the PC (polycarbonate) or PS (polystyrene) film that have hole to form its thickness between 50-500 micron (μ m), between 100-200 micron (μ m), the size of its mesh or membrane pores is not less than 0.2 micron (μ m) especially.
11. a kind of dry enzyme method biochip/test strip according to claim 1, it is characterized in that: described dried enzyme working electrode, contrast electrode, the test fluid diffuser screen, dried enzyme operation interval with biochip/test strip, its geometric configuration can be one or more shapes, comprises one of rectangle, trapezoidal, triangle, circle, semicircle, circular arc, ellipse, ellipse semicircle, elliptic arc, or its combination.
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| CN200910234106A CN101762628A (en) | 2009-10-10 | 2009-10-10 | Dry enzyme method biochip/test strip |
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| CN200910234106A CN101762628A (en) | 2009-10-10 | 2009-10-10 | Dry enzyme method biochip/test strip |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Microfluidic paper chip and manufacturing method for simultaneous detection of glucose, uric acid, triglyceride and cholesterol |
| CN103175871A (en) * | 2011-12-23 | 2013-06-26 | 长沙中生众捷生物技术有限公司 | Enzyme detection reagent of uric acid for electrochemical test strip |
| CN103235015A (en) * | 2012-10-24 | 2013-08-07 | 长沙理工大学 | Dry chemical test strip for quantitatively testing uric acid |
| WO2014153654A1 (en) * | 2013-03-25 | 2014-10-02 | Fpinnovations | Cellulose films with at least one hydrophobic or less hydrophilic surface |
| CN104614417A (en) * | 2014-02-17 | 2015-05-13 | 安阳师范学院 | Electrochemical method for detecting glycoprotein |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| US9664672B2 (en) | 2015-02-28 | 2017-05-30 | Rongbin ZENG | Dry chemical test strip with multiple layers of membranes based on concentration gradient |
| CN108489869A (en) * | 2018-04-10 | 2018-09-04 | 吉林工程技术师范学院 | A kind of kit and its application changing detection glucose in urine exception based on contact angle |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| WO2021018028A1 (en) * | 2019-08-01 | 2021-02-04 | Hangzhou Biotest Biotech Co., Ltd. | A detection device |
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2009
- 2009-10-10 CN CN200910234106A patent/CN101762628A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Microfluidic paper chip and manufacturing method for simultaneous detection of glucose, uric acid, triglyceride and cholesterol |
| CN103175871A (en) * | 2011-12-23 | 2013-06-26 | 长沙中生众捷生物技术有限公司 | Enzyme detection reagent of uric acid for electrochemical test strip |
| CN103175871B (en) * | 2011-12-23 | 2015-04-08 | 长沙中生众捷生物技术有限公司 | Enzyme detection reagent of uric acid for electrochemical test strip |
| CN103235015A (en) * | 2012-10-24 | 2013-08-07 | 长沙理工大学 | Dry chemical test strip for quantitatively testing uric acid |
| WO2014153654A1 (en) * | 2013-03-25 | 2014-10-02 | Fpinnovations | Cellulose films with at least one hydrophobic or less hydrophilic surface |
| CN104614417A (en) * | 2014-02-17 | 2015-05-13 | 安阳师范学院 | Electrochemical method for detecting glycoprotein |
| US9664672B2 (en) | 2015-02-28 | 2017-05-30 | Rongbin ZENG | Dry chemical test strip with multiple layers of membranes based on concentration gradient |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| CN108489869A (en) * | 2018-04-10 | 2018-09-04 | 吉林工程技术师范学院 | A kind of kit and its application changing detection glucose in urine exception based on contact angle |
| CN108489869B (en) * | 2018-04-10 | 2021-07-27 | 吉林工程技术师范学院 | A kit for detecting abnormal urine glucose based on the change of contact angle and its application |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| WO2021018028A1 (en) * | 2019-08-01 | 2021-02-04 | Hangzhou Biotest Biotech Co., Ltd. | A detection device |
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