CN101755779B - Fungicidal composition containing bromothalonil and propineb and application thereof - Google Patents

Abstract

The invention relates to a bactericidal composition containing bromothalonil and propeneb and its application, wherein the ratio of active components bromothalonil and propeneb is 20:1 to 1:100 by weight, and the composition The weight percent content of active components is 1-90%. The bactericidal composition is used to prevent and treat crop diseases caused by pathogenic fungi, not only can play a synergistic effect, reduce the cost of pesticide use, but also can greatly delay the speed of pathogenic bacteria developing drug resistance and prolong the service life of the medicament.

Description

A kind of bactericidal composition and application containing bromothalonil and propineb

technical field

The invention belongs to the technical field of pesticides, and in particular relates to a bactericidal composition containing bromothalonil and propineb, which is used to prevent and treat plant diseases caused by pathogenic fungi, such as downy mildew on crops such as grains, melons and fruits, and vegetables. Disease, blight, anthracnose, powdery mildew, rust etc.

technical background

Bromothalonil (Bromothalonil), the chemical name is 2-bromo-2-bromomethylglutaronitrile, is a new type of high-efficiency fungicide, which can be quickly absorbed by bacterial cells, and conducts in cells to interfere with its normal development, thereby achieving antibacterial , The role of sterilization. Bromothalonil has low toxicity and high safety. Bromothalonil, which is three times the concentration used, is milder to human skin irritation than soap, and has a wide bactericidal spectrum and wide application range. , A variety of fungi of the subphylum Deuteromycota and some bacterial diseases have good inhibitory and killing effects. The practical use of bromothalonil shows that it can not only prevent and control plant diseases and reduce yield loss, but at the same time, the drug itself can stimulate the activity of various enzymes in crops, increase photosynthesis, make the leaves of the plants dark green and strong, and improve the quality and quality of crops. Yield.

Bromothalonil can inhibit and eradicate germs very well and is an excellent fungicide, but long-term single use in large quantities will inevitably lead to the resistance of germs to it and greatly reduce the control effect.

Propineb (Pr o pi neb), chemical name zinc propylene bisdithiocarbamate, is a fast-acting, long-residue, broad-spectrum protective fungicide. Its bactericidal mechanism is not fixed, and there are many sites of action, mainly inhibiting the oxidation of pyruvate in pathogenic bacteria. It has good protective activity, but its therapeutic activity is not ideal, and its effect is not good when used alone.

At present, there are no reports about the compound product of bromothalonil and propeneb at home and abroad.

Contents of the invention

In view of the above technical problems, the present invention provides a high-efficiency bactericidal composition containing bromothalonil and propineb, which is used to prevent and treat crop diseases caused by pathogenic bacteria, which can not only play a synergistic role, reduce the cost of pesticide use, Moreover, it can greatly delay the speed of pathogenic bacteria developing drug resistance and prolong the service life of the drug.

The present invention is realized through the following technical solutions:

The invention relates to a bactericidal composition containing bromothalonil and propineb. The ratio by weight of the active ingredients bromothalonil to propineb is 20:1-1:100. Preferably, the ratio by weight of the bactericidal active substance bromothalonil to propineb is 1:1˜1:20.

The weight percentage of the active component of the fungicidal composition involved in the present invention is 1 to 90%, adding suitable known auxiliary agents and excipients, and making a dosage form suitable for agricultural use according to methods known to those skilled in the art. Preferred are wettable powders, suspension concentrates, and water-dispersible granules. The known auxiliary agents and excipients include dispersants, diffusing agents, defoamers, wetting agents, disintegrating agents and the like.

The fungicidal composition involved in the present invention can be used to prevent and treat various crop diseases, and is widely used in cereals, fruits, vegetables such as wheat, bananas, mangoes, grapes, apples, pears, strawberries, lychees, cucumbers, zucchini, peppers, eggplants, potatoes Prevention and control of downy mildew, blight, anthracnose, powdery mildew, rust and other diseases on crops such as tomato, kidney bean, lettuce and cabbage.

Beneficial effects of the present invention: one is that the compound use of bromothalonil and propineb can make the compound possess high protection activity and treatment and eradication activity at the same time, compared with a single agent, it can obviously improve the control effect, Prolonging the effective period, thus saving the amount of medication, medication cost and a lot of manpower and material resources, not only achieve twice the result with half the effort, but also greatly reduce the degree of harm to the environment due to the reduced amount of pesticide application, and reduce the burden on the social environment Therefore, it has good economic, social and environmental benefits. Second, due to the diversification of the bactericidal action sites of the compound agent, it can greatly delay the occurrence and development of pathogenic bacteria resistance, which is beneficial to prolong the service life of excellent fungicides.

Detailed ways

The bactericidal composition of bromothalonil and propineb of the present invention will be further described below in conjunction with the examples, but the present invention is not limited thereto.

Bioassay Example: Toxicity determination of bromothalonil and propineb complex against cucumber downy mildew

Indoor toxicity of single and mixed agents of bromothalonil and propineb to cucumber downy mildew was determined by plate leaf method. Test method: Put the cucumber leaves in a petri dish for moisturizing treatment, spray the pesticides according to the designed doses, inoculate cucumber downy mildew bacteria after 24 hours, and culture them in an incubator with moisture, investigate the control effect after 7 days, and use the disease index Calculate the control effect, use DPS data processing software for statistical analysis, calculate the EC50 of each agent, and use Sun Yunpei's method to calculate the co-toxicity coefficient (CTC) to evaluate the mixed effect. When the CTC value is greater than 120, the mixed agent has a synergistic effect, and when it is less than 80, it is Antagonism, additive effect between 80 and 120.

Toxicity index TI (B) = EC50 of standard agent A/EC50 of agent B*100

Actual toxicity index ATI(AB)=EC50 of A/EC50 of AB*100

Theoretical toxicity index TTI(AB)=TI(A)*A percentage in the mixture+TI(B)*B percentage in the mixture

Co-toxicity coefficient CTC＝ATI(AB)/TTI(AB)*100

The test results show that the combination of bromothalonil and propineb has good indoor toxicity to cucumber downy mildew, and the CTC values of all the ratios are more than 120, which shows that the two fungicides have great toxicity in this ratio. Good synergistic effect, especially when the mixing ratio is between 1:1 and 1:20. The experimental data are shown in Table 1 below.

Table 1 Comparison table of virulence determination of bromothalonil and propineb against cucumber downy mildew

Drugs to be tested Active ingredient ratio EC50(pp pm) Co-toxicity coefficient (CTC) Bromothalonil 17.76 Propineb 78.81 Bromothalonil: Propineb 20:1 14.49 127.26 Bromothalonil: Propineb 10:1 13.48 141.73 Bromothalonil: Propineb 5:1 12.14 167.98 Bromothalonil: Propineb 2:1 11.27 212.44 Bromothalonil: Propineb 1:1 11.08 261.62 Bromothalonil: Propineb 1:2 10.21 359.72 Bromothalonil: Propineb 1:5 9.36 535.30 Bromothalonil: Propineb 1:10 15.78 380.52 Bromothalonil: Propineb 1:20 26.33 257.21 Bromothalonil: Propineb 1:40 34.80 208.95 Bromothalonil: Propineb 1:80 47.56 158.96 Bromothalonil: Propineb 1:100 51.18 148.92

The fungicidal composition of the present invention is made into a suitable preparation to control downy mildew, blight, anthracnose, powdery mildew, rust, etc. It is found that the compound preparation has a good control effect on diseases, and is obviously better than the control effect of a single agent. The specific implementation is as follows:

Application Example 1: Drug efficacy test of bromothalonil and propineb compound to control Chinese cabbage downy mildew

Preparation of 30% bromothalonil · propineb wettable powder:

Bromothalonil 10%

Propineb 20%

Sodium Lauryl Sulfate 2%

Sodium lignosulfonate 4%

Alkyl naphthalene sulfonate 5%

Silica 10%

Light calcium carbonate balance

The above-mentioned active components, various auxiliary agents and fillers are fully mixed according to the formula ratio, and after being pulverized by an ultra-fine pulverizer, 30% bromothalonil·propenebine wettable powder can be obtained.

In the early stage of the onset of downy mildew of Chinese cabbage or when the central diseased strain is found, 30% bromothalonil · propene zinc wettable powder is diluted 800 times with water and sprayed once every 7 days, and sprayed 3 times in a row. Ten days after the third application, the control effect was 83.3%. Using 800 times liquid of 30% bromthalonil wettable powder and 400 times liquid of 70% propenezine wettable powder to control downy mildew of Chinese cabbage according to the same application method, the control effects were 78.2% and 63.2% respectively after 10 days of application.

Application Example 2: Drug efficacy test of compounding bromothalonil and propineb for preventing and treating early blight of tomato

Preparation of 70% bromothalonil · propineb wettable powder:

Bromothalonil 7%

Propineb 63%

Sodium Lauryl Sulfate 2%

Sodium Lignosulfonate 5%

Alkyl naphthalene sulfonate 4%

Silica 10%

Light calcium carbonate balance

The above-mentioned active components, various auxiliary agents and fillers are fully mixed according to the formula ratio, and after being pulverized by an ultrafine pulverizer, a 70% bromothalonil·propenebine wettable powder can be obtained.

At the initial stage of tomato early blight, dilute 70% bromothalonil-propenebine wettable powder by 1000 times and spray with water, spray once every 7 days, and spray 3 times in a row. The control effects were 89.3% and 83.6% 10 days and 15 days after the third spraying. Use 30% bromothalonil wettable powder to dilute and spray 800 times with water to prevent and control early blight of tomato, and the control effects are 83.1% and 78.3% respectively 10 days and 15 days after the application; For tomato early blight, the control effects were 73.8% and 68.4% 10 days and 15 days after treatment.

Application example 3: Drug efficacy test of bromothalonil and propineb compound to control potato late blight

Preparation of 25% bromothalonil · propine zinc water dispersible granules:

Bromothalonil 5%

Propineb 20%

Acrylic acid-acrylate copolymer 3%

Sodium Lauryl Sulfate 3%

Sodium Lignosulfonate 2%

Urea 10%

Kaolin The balance

Mix the above active components, dispersants, disintegrants and fillers uniformly according to the formula ratio, pulverize into wet powder by airflow, then add a certain amount of water, mix and extrude to granulate, and obtain 25% bromine after drying and sieving Bacterionitrile Propineb water dispersible granules.

In the early stage of disease onset, 25% bromothalonil·propenebine water dispersible granules were diluted with water to spray 1000 times to prevent and control potato late blight, spraying once every 7 days, and spraying 4 times in total. The control effects were 88.2% and 84.9% 10 days and 15 days after the fourth spraying. Use 1000 times liquid of 30% bromothalonil wettable powder to prevent and control late blight of potato, and the control effects are 84.2% and 80.4% after 10 days and 15 days after application; , the control effects were 78.2% and 75.9% respectively 10 days and 15 days after the medicine.

Application Example 4: Drug efficacy test of bromothalonil and propineb compound for preventing and treating mango anthracnose

Preparation of 30% bromothalonil·propenebine wettable powder

Bromothalonil 15%

Propineb 15%

Sodium alkylnaphthalene sulfonate 7%

Calcium Lignosulfonate 3%

Light calcium carbonate 20%

Silica 6%

Kaolin Balance

The above-mentioned components are uniformly mixed according to the formula ratio, and after the coarse powder is mixed, air-jet pulverization is uniformly mixed to obtain 30% bromothalonil·propenebine wettable powder.

During the flowering period of mango, when there is a lot of rain and it is easy to get sick, start to use 30% bromothalonil · propene zinc wettable powder to dilute 1200 times with water to prevent and control mango anthracnose. Spray once every 10 days, and spray 4 times in total. The control effects were 90.6% and 95.8% 7 days and 14 days after the fourth spraying. Mango anthracnose was controlled by using 1200 times liquid of 30% bromothalonil wettable powder, and the control effects were 88.7% and 92.2% respectively after 7 days and 14 days after treatment; 600 times liquid of 70% propinium wettable powder was used to control mango anthracnose , the control effects were 72.6% and 78.2% respectively 7 days and 14 days after the drug.

Application Example 5: Drug Efficacy Test of Combination of Bromothalonil and Propineb to Control Cucumber Powdery Mildew

Preparation of 42% Bromthalonil·Propineb Suspension Concentrate

Bromothalonil 2%

Propineb 40%

Sodium alkylnaphthalene sulfonate 8%

Glycerol 2%

Xanthan Gum 1%

Silicone defoamer 0.2%

Water balance

The above-mentioned components are uniformly mixed according to the formula ratio, ground and pulverized by a sand mill, and then mixed uniformly to obtain a 42% bromothalonil·propenebine suspension concentrate.

In the initial stage of the onset of cucumber powdery mildew, 42% bromothalonil·propenebine suspending agent was used to dilute and spray 700 times with water, and the control effects were 80.8% and 76.6% 10 days and 15 days after the application. Use 30% bromothalonil wettable powder 700 times liquid to prevent and control cucumber powdery mildew, the control effect is 75.4% and 72.2% after 10 days and 15 days respectively; use 70% propinium wettable powder 400 times liquid to control cucumber powdery mildew , the control effects were 67.2% and 64.9% respectively 10 days and 15 days after the medicine.

Application Example 6: Drug efficacy test of bromothalonil and propineb compound to control bean rust

Preparation of 18% Bromthalonil Propineb Suspension Concentrate

Bromothalonil 8%

Propineb 10%

Sodium alkylnaphthalene sulfonate 7%

Glycerol 2%

Xanthan Gum 0.5%

Silicone defoamer 0.2%

Water balance

The above-mentioned components are uniformly mixed according to the formula ratio, ground and pulverized by a sand mill, and then mixed uniformly to obtain 18% bromothalonil·propenebine suspension concentrate.

In the early stage of bean rust disease, 18% bromothalonil·propenebine suspending agent was diluted and sprayed with 750 times of water, and the control effect was 81.3% and 79.8% respectively 10 days and 15 days after spraying. 800 times liquid of 30% bromothalonil wettable powder was used to prevent and control bean rust, and the control effects were 79.2% and 72.7% 10 days and 15 days after the application; After 10 days and 15 days, the control effects were 65.2% and 61.4%, respectively.

Claims (6)

1. a bactericidal composition, is characterized in that containing Fungicidal active substance bromothalonil and Propineb, and the ratio of weight and number of described Fungicidal active substance bromothalonil and Propineb is 20:1～1:100.

2. according to bactericidal composition claimed in claim 1, the ratio of weight and number that it is characterized in that described Fungicidal active substance bromothalonil and Propineb is 1:1～1:20.

3. according to bactericidal composition claimed in claim 1, it is characterized in that described active component weight percentage is 1～90%.

4. according to bactericidal composition claimed in claim 1, it is characterized in that described active component can be mixed with wetting powder, suspending agent, water dispersible granules with known auxiliary agent and excipient.

5. the purposes of the plant disease that bactericidal composition claimed in claim 1 is caused by disease fungus for control.

According to bactericidal composition claimed in claim 1 for preventing and treating the purposes of downy mildew on cereal, melon and fruit, vegetable crop, epidemic disease, anthracnose, powdery mildew, rust.