CN101748046B - Full temperature micro volume blending incubation and hybridization instrument - Google Patents
Full temperature micro volume blending incubation and hybridization instrument Download PDFInfo
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- CN101748046B CN101748046B CN 200910262876 CN200910262876A CN101748046B CN 101748046 B CN101748046 B CN 101748046B CN 200910262876 CN200910262876 CN 200910262876 CN 200910262876 A CN200910262876 A CN 200910262876A CN 101748046 B CN101748046 B CN 101748046B
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- 238000009396 hybridization Methods 0.000 title claims abstract description 32
- 238000002156 mixing Methods 0.000 title claims abstract description 21
- 238000011534 incubation Methods 0.000 title claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 238000005057 refrigeration Methods 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 239000002086 nanomaterial Substances 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 9
- 238000012986 modification Methods 0.000 abstract description 4
- 230000004048 modification Effects 0.000 abstract description 4
- 239000007791 liquid phase Substances 0.000 abstract description 3
- 238000001816 cooling Methods 0.000 abstract 2
- 238000007385 chemical modification Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 5
- 239000002105 nanoparticle Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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Abstract
本发明提供一种全温微体积混匀孵育及杂交仪，包括封闭的箱腔(2)、热风扇(6)、冷风扇(1)、制冷压缩机(4)、温度传感器(11)和加热恒温系统(12)，所述热风扇(6)、冷风扇(1)和温度传感器(11)设置在所述箱腔(2)内，其特征在于：还包括电机(10)和圆盘(8)，所述圆盘(8)设置在所述箱腔(2)内，与所述电机(10)连接，所述圆盘(8)上垂直于电机轴的方向设置有可插放离心管的圆洞。本发明产品提供了更广泛的温度范围，给纳米材料表面的化学、生物大分子修饰及杂交提供适宜的温度，保证了生物大分子的活性和杂交的精度，还特别适用于纳米材料在小体积液相环境中的混匀悬浮，保证了纳米材料表面修饰的均匀性。
The invention provides a full-temperature micro-volume mixing incubation and hybridization instrument, comprising a closed chamber (2), a heating fan (6), a cooling fan (1), a refrigeration compressor (4), a temperature sensor (11) and Heating and constant temperature system (12), the hot fan (6), cooling fan (1) and temperature sensor (11) are arranged in the box cavity (2), characterized in that: it also includes a motor (10) and a disc (8), the disc (8) is arranged in the box cavity (2) and connected with the motor (10), and the disc (8) is provided with insertable The round hole of the centrifuge tube. The product of the present invention provides a wider temperature range, provides a suitable temperature for the chemical modification and hybridization of the surface of nanomaterials, biomacromolecules, ensures the activity of biomacromolecules and the accuracy of hybridization, and is especially suitable for nanomaterials in small volumes. The mixing and suspension in the liquid phase environment ensures the uniformity of the surface modification of nanomaterials.
Description
Technical field
The invention belongs to biological material analysis quasi-instrument field, particularly have the biomacromolecule on the micro-nano granules surface of requirement to modify and needed full temperature micro volume blending incubation of molecular hybridization and hybridization instrument temperature.
Background technology
Nano particle is the nano material that research is maximum in the biomedicine, application is the widest, and the character of many uniquenesses is arranged.Research at present more has carbon, silicon, quantum dot and superparamagnetic nanomaterial etc.These materials have like characteristics such as small-size effect, quantum size effect, surface effects, macro quanta tunnel effect, volume effects, are widely used in the pathogen detection that replaces the existing chip detection technique, the mark early diagnosis of tumour, all respects such as external visual detection of disease.
To nano particle be applied to biomedical sector, just must carry out necessary chemistry and the modification of biomacromolecule, like protein, carbohydrate and oligonucleotide probe modification etc. to nano grain surface.The effect of these biomacromolecules that utilization is modified and other molecule of its specific recognition, the function of this molecule of performance identification.Because the singularity of Bioexperiment, the chemical reaction of nano grain surface needs certain temperature; To biomacromolecule itself, like protein and nucleic acid etc., can be by extraneous microbiological degradation or owing to the action of temperature function that loses activity; The perhaps specific combination ability between the biomacromolecule, like antibody and antigen, nucleic acid hybridization etc., the height of temperature are the deciding factors that whether combines.Therefore these modifications of nano particle need be able to carried out in the artificial environment of accurate temperature controlling.
At present; In the prior art; Chip, Southern or the Northern hybridization carried out based on slide and Hybond membrane are crossover process in one plane, and the bigger needed hybridization solution of its volume is many, places hybridization bottle (50mL); In commercially available hybrid heater, impel hybridization solution constantly to mix by the gravity of liquid self, evenly hybridization with the rotation that do not stop.Be different from above-mentioned hybridization conditions, the biomacromolecule of nano-material surface is modified and how hybridizes required hybridization reaction solution volume at 20-100 μ L, and mostly its reaction vessel is the centrifuge tube of 200 μ L-1000 μ L.There is not special-purpose hybrid device at present on the market based on the centrifuge tube of 200 μ L-1000 μ L; And hybrid pipe is parallel with motor shaft in commercially available Hybridization Oven; The hybridization solution self gravitation of small volume is little in the rotation crossover process, or the effect of capillarity can not utilize self gravitation to flow, and nano particle will soon sedimentation; Thereby cause mixing inhomogeneous, badly influenced that particulate is modified or hybridization efficiency.
Summary of the invention
The object of the invention is exactly the deficiency that will overcome commercially available instrument; Providing a kind of both can accurately control to meet all temps demand that nano-material surface chemistry and biomolecules are modified and hybridized temperature; Can make liquid-flow fully again, make uniform full temperature micro volume blending incubation of liquid-phase mixing and hybridization instrument.
The objective of the invention is to realize like this: a kind of full temperature micro volume blending incubation and hybridization instrument; Comprise closed case chamber, Hot-air fan, thermantidote, refrigeration compressor, TP and heating constant-temperature system; Said Hot-air fan, thermantidote and TP are arranged in the said case chamber, also comprise motor and disk, and said disk is arranged in the said case chamber; Be connected with said motor, the direction perpendicular to motor shaft on the said disk is provided with the round hole that can insert centrifuge tube.Bonded heating constant-temperature system on the case chamber is changed by the TP sense temperature, and by chilled air fan and hot blast fan the temperature in the chamber is controlled, and TR is 0 ℃～99.9 ℃, and temperature control precision is ± 0.1 ℃, and temperature uniformity is ± 0.2 ℃.
Be arranged on outermost casing in order to make the compactness rational in infrastructure of full temperature micro volume blending incubation of the present invention and hybridization instrument, also to comprise, said case chamber, refrigeration compressor, heating constant-temperature system and motor all are arranged in the said casing.
Convenient in order to place centrifuge tube, said case chamber is provided with the double thermal insulation glass door, and said double thermal insulation glass door passes said casing and links to each other with the external world.
In order to make product internal heat dissipating of the present invention fast, also be provided with heat abstractor in the said casing.
For easy to operate, also comprise device control unit in the product of the present invention, device control unit links to each other with all electric elements in the product, temperature controllable and motor speed, said device control unit is arranged on the said casing.
In order to make disc rotation speed 0-60rpm controlled, said motor is a DC speed-reducing.
Fall from disk when preventing that centrifuge tube from forwarding low level to, said disk is provided with the elastic band that prevents that centrifuge tube from coming off.
During use, the centrifuge tube that nano material and to be finished or hybridization solution are housed is inserted disk, fix with elastic band; Behind power connection, adjustment control as required, controlled temperature and rotating speed (0～60rpm); The slow uniform rotation of driven by motor disk, centrifuge tube will periodically be put upside down.Liquid in the pipe and nano material will owing to the continuous transform stream of gravity to, fully accomplish the purpose of modifying and hybridizing to reach liquid with the abundant mixing of nano material.
Beneficial effect of the present invention is:
Full temperature micro volume blending incubation of the present invention and hybridization instrument compared with prior art have following characteristics:
One, product of the present invention provides TR widely, modifies for the chemistry of nano-material surface and biomacromolecule, reaches hybridization suitable temperature is provided, and has guaranteed the activity of biomacromolecule and the precision of hybridization;
Two, product of the present invention is applicable to hatching of small volume reaction liquid system and hybridizes, and has practiced thrift reagent and cost; Be specially adapted to the maintenance mixing suspended state of nano material in the small volume liquid phase environment, guaranteed fully contacting of the reagent in the solution and nano-material surface, guaranteed the homogeneity that nano-material surface is modified;
Three, product of the present invention is filled up the market vacancy based on micro volume blending incubation and hybridization instrument.
Description of drawings
Fig. 1 is the structural representation of full temperature micro volume blending incubation of the present invention and hybridization instrument.
Embodiment
Through the mode of embodiment, technical scheme of the present invention is elaborated below, but protection scope of the present invention is not limited to said embodiment.
As shown in Figure 1; A kind of full temperature micro volume blending incubation and hybridization instrument comprise closed case chamber 2, Hot-air fan 6, thermantidote 1, refrigeration compressor 4, TP 11 and heating constant-temperature system 12, and said Hot-air fan 6, thermantidote 1 and TP 11 are arranged in the said case chamber 2; Also comprise DC speed-reducing 10 and disk 8; Said disk 8 is arranged in the said case chamber 2, is connected with said DC speed-reducing 10, and the direction perpendicular to motor shaft on the said disk 8 is provided with the round hole that can insert centrifuge tube; Said disk 8 is provided with the elastic band 9 that prevents that centrifuge tube from coming off; During use, the centrifuge tube that nano material and to be finished or hybridization solution are housed is inserted in the round hole on the disk, centrifuge tube is inserted in the disk with the mode of pipe shaft perpendicular to motor shaft; The outside is fixed with elastic band 9, falls from disk when preventing that centrifuge tube from forwarding low level to; Be combined with heating constant-temperature system 12 on the case chamber 2; Temperature by in the variation of TP 11 sense temperature, chilled air fan 1 and 6 pairs of chambeies of hot blast fan is controlled; TR is 0 ℃～99.9 ℃, and temperature control precision is ± 0.1 ℃, and temperature uniformity is ± 0.2 ℃;
Also comprise being arranged on outermost casing 7, said case chamber 2, refrigeration compressor 4 and DC speed-reducing 10 all are arranged in the said casing 7; Said case chamber 2 is provided with double thermal insulation glass door 3, and said double thermal insulation glass door 3 passes said casing 7 and links to each other with the external world; Also be provided with heat abstractor 13 in the said casing 7; Also comprise device control unit 5, said device control unit 5 is arranged on the said casing 7.
Behind power connection, adjustment control 5 as required, controlled temperature and rotating speed, and DC speed-reducing 10 drives disk 8 slow uniform rotation, and centrifuge tube will periodically be put upside down.Liquid in the pipe and nano material will owing to the continuous transform stream of gravity to, fully accomplish the purpose of modifying and hybridizing to reach liquid with the abundant mixing of nano material.
Claims (4)
1. full temperature micro volume blending incubation and hybridization instrument; Comprise closed case chamber (2), Hot-air fan (6), thermantidote (1), refrigeration compressor (4), TP (11) and heating constant-temperature system (12); Said Hot-air fan (6), thermantidote (1) and TP (11) are arranged in the said case chamber (2); It is characterized in that: also comprise motor (10) and disk (8); Said disk (8) is arranged in the said case chamber (2), is connected with said motor (10), and the direction that said disk (8) is gone up perpendicular to motor shaft is provided with the round hole that inserts centrifuge tube; Said motor (10) is a DC speed-reducing; Also comprise device control unit (5), said device control unit (5) is arranged on the said casing (7).
2. full temperature micro volume blending incubation according to claim 1 and hybridization instrument; It is characterized in that: also comprise being arranged on outermost casing (7); Said case chamber (2), refrigeration compressor (4), heating constant-temperature system (12) and motor (10) all are arranged in the said casing (7); Said case chamber (2) is provided with double thermal insulation glass door (3), and said double thermal insulation glass door (3) passes said casing (7) and links to each other with the external world.
3. full temperature micro volume blending incubation according to claim 1 and hybridization instrument; It is characterized in that: also comprise being arranged on outermost casing (7); Said case chamber (2), refrigeration compressor (4), heating constant-temperature system (12) and motor (10) all are arranged in the said casing (7), also are provided with heat abstractor (13) in the said casing (7).
4. according to described full temperature micro volume blending incubation of the arbitrary claim of claim 1～3 and hybridization instrument, it is characterized in that: said disk (8) is provided with the elastic band (9) that prevents centrifuge tube and come off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910262876 CN101748046B (en) | 2009-12-14 | 2009-12-14 | Full temperature micro volume blending incubation and hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910262876 CN101748046B (en) | 2009-12-14 | 2009-12-14 | Full temperature micro volume blending incubation and hybridization instrument |
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| Publication Number | Publication Date |
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| CN101748046A CN101748046A (en) | 2010-06-23 |
| CN101748046B true CN101748046B (en) | 2012-08-15 |
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| CN 200910262876 Expired - Fee Related CN101748046B (en) | 2009-12-14 | 2009-12-14 | Full temperature micro volume blending incubation and hybridization instrument |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992945B (en) * | 2014-05-19 | 2015-09-23 | 东南大学 | A kind of constant-temperature incubation hybrid device |
| CN106872432A (en) * | 2017-03-30 | 2017-06-20 | 杭州晶格科学仪器有限公司 | A kind of fluorescence detection device and temperature control method with temperature control mechanism |
| CN109486650B (en) * | 2018-09-20 | 2022-03-25 | 宁波新芝生物科技股份有限公司 | Microorganism growth curve tester |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19925154A1 (en) * | 1998-06-02 | 1999-12-09 | Kendro Lab Prod Gmbh | Controlling internal temperature of e.g. an incubator cabinet equipped with heater and coolant compressor |
| CN1401760A (en) * | 2002-09-09 | 2003-03-12 | 梁朝 | Energy-saving magnetic suspension nucleic acid hybridizing furnace |
| CN2727185Y (en) * | 2004-06-09 | 2005-09-21 | 李勇 | Incubation device with ice bath |
| CN2789267Y (en) * | 2005-03-22 | 2006-06-21 | 肖先进 | Molecular hybridization instrument |
| CN101063078A (en) * | 2007-04-20 | 2007-10-31 | 博奥生物有限公司 | Biological chip hybrid cleaning device |
| CN201062262Y (en) * | 2007-06-26 | 2008-05-21 | 北京和利时系统工程股份有限公司 | Biochip hybridization instrument |
-
2009
- 2009-12-14 CN CN 200910262876 patent/CN101748046B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19925154A1 (en) * | 1998-06-02 | 1999-12-09 | Kendro Lab Prod Gmbh | Controlling internal temperature of e.g. an incubator cabinet equipped with heater and coolant compressor |
| CN1401760A (en) * | 2002-09-09 | 2003-03-12 | 梁朝 | Energy-saving magnetic suspension nucleic acid hybridizing furnace |
| CN2727185Y (en) * | 2004-06-09 | 2005-09-21 | 李勇 | Incubation device with ice bath |
| CN2789267Y (en) * | 2005-03-22 | 2006-06-21 | 肖先进 | Molecular hybridization instrument |
| CN101063078A (en) * | 2007-04-20 | 2007-10-31 | 博奥生物有限公司 | Biological chip hybrid cleaning device |
| CN201062262Y (en) * | 2007-06-26 | 2008-05-21 | 北京和利时系统工程股份有限公司 | Biochip hybridization instrument |
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