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CN101745519A - Agricultural safety production operating method by utilizing low cadmium-accumulation Chinese cabbage - Google Patents

Agricultural safety production operating method by utilizing low cadmium-accumulation Chinese cabbage Download PDF

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CN101745519A
CN101745519A CN 200810229328 CN200810229328A CN101745519A CN 101745519 A CN101745519 A CN 101745519A CN 200810229328 CN200810229328 CN 200810229328 CN 200810229328 A CN200810229328 A CN 200810229328A CN 101745519 A CN101745519 A CN 101745519A
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cadmium
chinese cabbage
soil
beijing
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刘维涛
周启星
刘睿
孙约兵
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Institute of Applied Ecology of CAS
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Abstract

本发明涉及镉污染土壤环境的农作物安全生产技术,具体地说是一种利用低积累镉的大白菜品种北京新3号在镉污染土壤安全生产的方法。在镉污染土壤种植低积累镉的大白菜,利用低积累镉大白菜地上部排斥吸收富集污染土壤中的镉,根部固定吸附土壤中的镉,从而阻止其向上转运到地上部。地上部作为安全蔬菜食用或出售,根部给予其他妥善处理,从而即可以保障蔬菜食品安全,又可以利用非食用部位(根部)固定吸附土壤中大量的镉;通过反复种植该种蔬菜作物,重复上述过程,就可以连续提取污染土壤中过量的镉,并且大白菜可食部位的镉含量也满足无公害安全蔬菜的标准。该方法具有费用低,不破坏土壤的理化性质,无二次污染,且具有在污染土壤中边生产、边治理等优点。The invention relates to a safe production technology of crops in cadmium-polluted soil environment, in particular to a method for safe production of Chinese cabbage variety Beijing Xin No. 3 with low accumulation of cadmium in cadmium-polluted soil. Plant Chinese cabbage with low accumulation of cadmium in cadmium-contaminated soil, use the aboveground part of Chinese cabbage with low accumulation of cadmium to repel and absorb the cadmium in the polluted soil, and the root to fix and absorb the cadmium in the soil, thereby preventing its upward transport to the aboveground part. The aboveground part is eaten or sold as a safe vegetable, and the root is given other proper treatment, so that the food safety of the vegetable can be guaranteed, and a large amount of cadmium in the soil can be fixed and adsorbed by using the non-edible part (root); by repeatedly planting this kind of vegetable crop, repeat the above The excessive cadmium in the polluted soil can be extracted continuously, and the cadmium content in the edible parts of Chinese cabbage also meets the standard of pollution-free and safe vegetables. The method has the advantages of low cost, no damage to the physical and chemical properties of the soil, no secondary pollution, and the advantages of simultaneous production and simultaneous treatment in the polluted soil.

Description

利用镉低积累大白菜实施农业安全生产的方法 The method of implementing agricultural safety production by using Chinese cabbage with low cadmium accumulation

技术领域technical field

本发明涉及镉污染土壤环境的农作物安全生产技术,具体地说是一种利用镉低积累型大白菜在镉污染土壤安全生产的方法,它是利用低积累重金属镉大白菜的排异机理,在中低程度镉污染土壤中仍能达成安全生产的方法。The present invention relates to the safe production technology of crops in cadmium polluted soil environment, specifically a method for safe production of cadmium-contaminated Chinese cabbage in cadmium-contaminated soil, which uses the rejection mechanism of low-accumulated heavy metal cadmium Chinese cabbage, in A safe production method can still be achieved in medium and low levels of cadmium-contaminated soil.

背景技术Background technique

近年来,随着我国工农业生产的迅速发展,我国土壤环境中的镉(镉)污染日益严重。众多人类活动,诸如大规模开采冶炼、电镀、污水灌溉和施用污泥以及含镉农药和化肥的使用等,使得大量的镉释放累积到环境中,造成广泛和严重的土壤镉污染[文献1:周启星,黄国宏.环境生物地球化学及全球环境变化[M].北京:科学出版社,2000.]。据报道,江西大余矿区、沈阳市张士灌区、上海的沙川灌区等都是典型的重金属镉污染区,严重污染面积(生产出粮食的镉含量超过食品卫生标准数倍甚至数十倍)占10%以上[文献2:徐爱春,陈益泰.镉污染土壤根际环境的调节与植物修复研究进展.中国土壤与肥料,2007,(2):1~6.]。污染土壤中过量的镉不仅直接威胁人类的生存环境和食品安全,还可以通过直接接触或者通过食物链在人体中富集,产生慢性中毒,影响体内钙和磷的代谢,引起肾、骨、肝的病理变化,诱发骨骼、消化系统、泌尿系统的多种疾病,并可导致恶性肿瘤。因此,土壤环境中的镉污染亟待修复治理。In recent years, with the rapid development of my country's industrial and agricultural production, the pollution of cadmium (cadmium) in my country's soil environment has become increasingly serious. Numerous human activities, such as large-scale mining and smelting, electroplating, sewage irrigation and sludge application, and the use of cadmium-containing pesticides and fertilizers, have released a large amount of cadmium into the environment, causing extensive and serious soil cadmium pollution [Document 1: Zhou Qixing, Huang Guohong. Environmental biogeochemistry and global environmental change [M]. Beijing: Science Press, 2000.]. According to reports, the Dayu Mining Area in Jiangxi, the Zhangshi Irrigation Area in Shenyang City, and the Shachuan Irrigation Area in Shanghai are all typical areas polluted by heavy metal cadmium. More than 10% [Document 2: Xu Aichun, Chen Yitai. Research progress on regulation of cadmium-contaminated soil rhizosphere environment and phytoremediation. China Soil and Fertilizer, 2007, (2): 1-6.]. Excessive cadmium in polluted soil not only directly threatens the human living environment and food safety, but also can be enriched in the human body through direct contact or through the food chain, resulting in chronic poisoning, affecting the metabolism of calcium and phosphorus in the body, and causing damage to the kidneys, bones, and liver. Pathological changes can induce various diseases of bone, digestive system and urinary system, and can lead to malignant tumors. Therefore, the cadmium pollution in the soil environment needs to be remediated urgently.

目前可用于修复治理土壤重金属污染的技术很多,传统的重金属污染土壤治理主要采用土壤固化、玻璃化、淋滤法、洗土法、客土法、电化学法等物理或化学方法等。这些方法存在着以下缺点:(1)成本高,一公顷的重金属污染土地往往高达数千万美圆,而且难以大规模使用;(2)破坏土壤生态环境,导致土壤结构丧失、土壤生物活性下降和土壤肥力退化,往往需要进行二次处理;(3)二次污染,运用物理、化学方法对重金属污染土壤修复的过程往往又会造成对土壤的再次污染。因此,一般很难在面积大、程度轻的污染土壤修复治理中实际推广应用。At present, there are many technologies that can be used to remediate heavy metal pollution in soil. Traditional heavy metal contaminated soil treatment mainly uses physical or chemical methods such as soil solidification, vitrification, leaching, soil washing, guest soil method, and electrochemical method. These methods have the following disadvantages: (1) the cost is high, and one hectare of heavy metal-contaminated land is often as high as tens of millions of dollars, and it is difficult to use on a large scale; (2) the soil ecological environment is destroyed, resulting in loss of soil structure, soil biological activity and The degradation of soil fertility often requires secondary treatment; (3) secondary pollution, the process of using physical and chemical methods to remediate heavy metal-contaminated soil often causes re-pollution of the soil. Therefore, it is generally difficult to practically popularize and apply it in the remediation and treatment of large-scale and lightly polluted soil.

与这些传统的治理技术相比,自上世纪80年代兴起的植物修复技术作为一种的原位绿色修复技术,具有费用低廉、不破坏环境、不造成二次污染、易于为人们所接受等优点,逐渐成为环境科学领域的研究与应用热点[文献3:周启星,宋玉芳.污染土壤修复原理与方法[M]北京:科学出版社,2004.]。但是植物修复也存在以下明显缺点和不足:(1)超积累植物生物量较小且具有明显的地域分布;(2)修复周期过长,通常需要上百年;(3)植物修复污染土壤的深度有限,仅能修复植物根系深度的土壤。因此,植物修复技术极大程度的受到这些因素的限制,目前尚难以在我国实际推广(文献4:刘维涛,张银龙,陈喆敏等.矿区绿化树木对镉和锌的吸收与分布[J].应用生态学报,2008,19(4)):752~756.)。鉴于我国实际国情,将大面积中轻度污染农田停止农作,进行长时间的植物修复或采用价格昂贵的工程修复显然是不现实的。Compared with these traditional treatment technologies, phytoremediation technology, which emerged in the 1980s as an in-situ green restoration technology, has the advantages of low cost, no damage to the environment, no secondary pollution, and easy acceptance by people. , has gradually become a research and application hotspot in the field of environmental science [Document 3: Zhou Qixing, Song Yufang. Principles and methods of contaminated soil remediation [M] Beijing: Science Press, 2004.]. However, phytoremediation also has the following obvious shortcomings and deficiencies: (1) The biomass of hyperaccumulative plants is small and has obvious geographical distribution; (2) The remediation period is too long, usually hundreds of years; (3) The depth of phytoremediation of polluted soil Limited, can only remediate soil to the root depth of plants. Therefore, phytoremediation technology is greatly limited by these factors, and it is still difficult to promote it in my country (Document 4: Liu Weitao, Zhang Yinlong, Chen Zhemin, etc. Absorption and distribution of cadmium and zinc by green trees in mining areas[J]. Journal of Applied Ecology, 2008, 19(4)): 752~756.). In view of my country's actual national conditions, it is obviously unrealistic to stop farming in large areas of mildly polluted farmland for long-term phytoremediation or to use expensive engineering repairs.

科学家研究发现,植物对镉的吸收积累不仅存在种间差异,也存在显著的种内差异。从而,筛选和培育排异和低积累土壤镉等有害元素的农作物品种,保证农产品的安全生产成为当前最为有效和合理的途径之一。重金属低积累作物筛选和培育的研究已经成为当前国内外研究的一个热点,众多研究结果表明水稻(Oryza sativa L.)、小麦(Triticum aestivum L.)、大麦(Hordeum vulgare L.)、玉米(Zeamays L.)和花生(Arachis hypogaea L.)等作物不同基因型积累与分布重金属存在显著差异,并筛选出了相应的重金属低积累基因型(文献5:Arao T.,Ishikawa S.Genotypic differences in cadmium concentration and distribution of soybeans and rice[J].Japanese Agricultural Research Quarterly.2006,40,21~30;文献6:Zhang G.P.,Fukami M.,Sekimoto H.Genotypic differences in effects of cadmium on growth andnutrient compositions in wheat[J].Journal of Plant Nutrition,2000,23:1337~1350;文献7:Chen F.,Dong J.,Wang F.,et al.Identification of barley genotypes with low grainCd accumulation and its interaction with four microelements[J].Chemosphere,2007,67,2082~2088;文献8:Florijn P.L.,Van Beusichem M.L.Uptake and distribution ofcadmium in maize inbred lines[J].Plant and Soil,1993,150:25-32;文献9:McLaughlinM.J.,Bell M.J.,Wright G.C.,et al.Uptake and partitioning of cadmium by cultivars ofpeanut(Arachis hypogaea L.)Plant and Soil,2000,222(1-2):51~58.)。然而,目前关于低积累重金属蔬菜品种的研究还较少,低积累镉的大白菜品种尚未见报道。有研究表明,进入人体的镉70%来自于受镉污染的蔬菜(文献10:Ryan J.A.,PahrenH.R.,Lucas J.B.Controlling cadmium in the human chain:A review and rationalebased on health effects[J].Environmental Research.1982,28:251~302.)。因此,筛选并利用重金属低积累大白菜在中、轻度的镉污染土壤的安全生产具有理论和现实意义,也引起了有关部门的高度关注。Scientists have found that there are not only interspecific differences in the uptake and accumulation of cadmium by plants, but also significant intraspecific differences. Therefore, screening and cultivating crop varieties with low accumulation of soil cadmium and other harmful elements to ensure the safe production of agricultural products has become one of the most effective and reasonable ways at present. The research on the screening and breeding of crops with low accumulation of heavy metals has become a hotspot in the current domestic and foreign research. Numerous research results have shown that rice (Oryza sativa L.), wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), corn (Zeamays L.) and peanut (Arachis hypogaea L.) and other crops such as peanut (Arachis hypogaea L.) have significant differences in the accumulation and distribution of heavy metals in different genotypes, and the corresponding genotypes with low accumulation of heavy metals were screened out (Reference 5: Arao T., Ishikawa S.Genotypic differences in cadmium concentration and distribution of soybeans and rice[J]. Japanese Agricultural Research Quarterly. 2006, 40, 21~30; Document 6: Zhang G.P., Fukami M., Sekimoto H.Genotypic differences in effects of cadmium on growth and nutrient at hepositions[ J]. Journal of Plant Nutrition, 2000, 23: 1337~1350; Literature 7: Chen F., Dong J., Wang F., et al. Identification of barley genotypes with low grainCd accumulation and its interaction with four microelements[J] ]. Chemosphere, 2007, 67, 2082~2088; Document 8: Florijn P.L., Van Beusichem M.L. Uptake and distribution of cadmium in maize inbred lines[J]. Plant and Soil, 1993, 150: 25-32; Document 9: McLaughlinM. J., Bell M.J., Wright G.C., et al. Uptake and partitioning of cadmium by cultivars ofpeanut (Arachis hypogaea L.) Plant and Soil, 2000, 222(1-2): 51~58.). However, there are still few studies on vegetable varieties with low accumulation of heavy metals, and Chinese cabbage varieties with low accumulation of cadmium have not been reported. Studies have shown that 70% of cadmium entering the human body comes from cadmium-contaminated vegetables (document 10: Ryan J.A., PahrenH.R., Lucas J.B. Controlling cadmium in the human chain: A review and rationale based on health effects[J].Environmental Research. 1982, 28: 251-302.). Therefore, screening and using Chinese cabbage with low accumulation of heavy metals for safe production in moderately and slightly cadmium-polluted soils has theoretical and practical significance, and has also attracted great attention from relevant departments.

大白菜(Brassica Pekinensis L.)属于十字花科芸苔属植物,是典型的低温长日照二年生植物,为东北地区乃至全国普遍栽培的一种大众化蔬菜,也是十分重要的经济作物。据统计,大白菜是我国栽培面积最大的蔬菜作物,年种植面积267万公倾左右,占全国蔬菜作物总种植面积的15%,年总产量超过1亿吨,占全国蔬菜总产量的18.8%(文献12:农业部.2005年全国各地蔬菜播种面积和产量[J].中国蔬菜,2007,(1):40~41.)。因此,利用低积累镉大白菜在污染土壤中安全生产具有紧迫性和必要性。Chinese cabbage (Brassica Pekinensis L.) belongs to the Brassica genus of the Brassica family. It is a typical low-temperature and long-sun biennial plant. It is a popular vegetable widely cultivated in Northeast China and even the whole country. According to statistics, Chinese cabbage is the vegetable crop with the largest planting area in my country, with an annual planting area of about 2.67 million hectares, accounting for 15% of the total planting area of vegetable crops in the country, and a total annual output of more than 100 million tons, accounting for 18.8% of the total national vegetable output (Document 12: Ministry of Agriculture. Vegetable planting area and output across the country in 2005 [J]. Chinese Vegetables, 2007, (1): 40-41.). Therefore, it is urgent and necessary to safely produce Chinese cabbage with low accumulation of cadmium in polluted soil.

大白菜原产于我国,已经有1500多年的栽培历史,种质资源极其丰富,利用大白菜排异机制在镉污染农田土壤安全生产和实践具有以下优点:(1)大白菜在我国栽培历史悠久并且种质资源丰富,为筛选工作奠定了坚实的基础;(2)能够在镉污染土壤安全生产的同时修复镉污染,从而达到边生产边治理之目的,一举两得;(3)大白菜味道鲜美,营养丰富,有“百菜之王”之美誉,深受百姓喜爱;(4)我国中医认为,大白菜微寒味甘,有养胃生津、除烦解渴、利尿通便、化痰止咳、清热解毒之功效(5)大白菜是我国重要的经济作物,已经出口日本、韩国、东南亚、欧盟和北美诸国,促进了我国经济发展和出口创汇;(5)我国人民在长期的农业生产中,积累了丰富的大白菜栽培管理经验,使得低积累镉大白菜在镉污染土壤中的安全生产有了充分的农业技术保障。因此,从众多大白菜品种中筛选出低积累镉的大白菜品种并用于污染土壤的安全生产是完全可行的。Chinese cabbage is native to China and has a cultivation history of more than 1,500 years. The germplasm resources are extremely rich. Using the rejection mechanism of Chinese cabbage in the safe production and practice of cadmium-contaminated farmland soil has the following advantages: (1) Chinese cabbage has a long history of cultivation in China And the germplasm resources are abundant, which has laid a solid foundation for the screening work; (2) it can repair cadmium pollution while producing safely in cadmium-contaminated soil, so as to achieve the purpose of controlling while producing, killing two birds with one stone; (3) Chinese cabbage is delicious, Rich in nutrition, it has the reputation of "the king of all vegetables", and is deeply loved by the people; (4) Chinese medicine believes that Chinese cabbage is slightly cold and sweet, and has the functions of nourishing the stomach and promoting body fluid, eliminating annoyance and quenching thirst, diuresis and laxative, reducing phlegm and relieving cough, and clearing away heat. The effect of detoxification (5) Chinese cabbage is an important economic crop in China, which has been exported to Japan, South Korea, Southeast Asia, the European Union and North American countries, which has promoted China's economic development and export earnings; (5) Chinese people have long-term agricultural production, The rich experience in Chinese cabbage cultivation and management has been accumulated, so that the safe production of low-cadmium-accumulated Chinese cabbage in cadmium-contaminated soil has sufficient agricultural technical guarantee. Therefore, it is entirely feasible to screen out the Chinese cabbage varieties with low accumulation of cadmium from many Chinese cabbage varieties and use them in the safe production of polluted soil.

发明内容Contents of the invention

本发明的目的是提供一种利用低积累镉的大白菜在镉污染土壤安全生产的方法,利用低积累镉的大白菜北京新3号在镉污染土壤中安全生产。The object of the present invention is to provide a method for safe production of Chinese cabbage with low cadmium accumulation in cadmium-contaminated soil, and safe production of Chinese cabbage Beijing Xin No. 3 with low cadmium accumulation in cadmium-contaminated soil.

为实现上述目的,本发明的技术方案为:To achieve the above object, the technical solution of the present invention is:

利用镉低积累大白菜北京新3号农业安全生产的方法:在镉污染土壤中种植低积累镉的大白菜北京新3号,边生产边修复,实现去除土壤中过量镉的目的。The method of safe agricultural production using Chinese cabbage Beijing Xin No. 3 with low cadmium accumulation: plant Beijing Xin No. 3 Chinese cabbage with low accumulation of cadmium in cadmium-contaminated soil, and restore it while producing, so as to achieve the purpose of removing excess cadmium in the soil.

所述在镉污染土壤中种植的大白菜为北京新3号,该大白菜为低积累重金属镉的蔬菜作物。所述在镉污染土壤中种植该大白菜,是指将该品种大白菜籽直接穴播于镉污染土壤中,每穴3-6粒白菜籽,穴间距10-60cm,垄间距10-60cm。在镉污染土壤中种植的该大白菜,采用室外盆栽栽培和野外大田栽培,在盆栽试验中种植大白菜北京新3号,是指将北京新3号种籽直接播种于塑料盆内(

Figure G2008102293285D0000031
Figure G2008102293285D0000032
H=15cm),每盆最后定苗2株。投加镉的浓度(mg/kg)为0、1、2.5和5。定期浇水,使土壤含水量保持在田间持水量的40-90%。在镉污染土壤中种植该大白菜,该大白菜地上部排斥吸收污染土壤中镉,同时根部固定、吸附镉,从而阻止其向地上部转移;当大白菜生长60-85天成熟时,将大白菜地上部从污染土壤上收获,将根部移除后妥善处理,待下个生长期再种植第二茬,重复上述操作。所述的每次收割后的大白菜根进行专门集中处理,处理的方法包括焚烧、萃取回收铅或压缩后作无害化填埋。The Chinese cabbage planted in cadmium-contaminated soil is Beijing Xin No. 3, which is a vegetable crop with low accumulation of heavy metal cadmium. The planting of the Chinese cabbage in the cadmium-contaminated soil refers to directly sowing the Chinese cabbage seeds of the variety in the cadmium-contaminated soil, with 3-6 cabbage seeds per hole, the hole spacing is 10-60cm, and the ridge spacing is 10-60cm. The Chinese cabbage planted in cadmium-contaminated soil adopts outdoor pot cultivation and field field cultivation. Planting Chinese cabbage Beijing No. 3 in the pot experiment means directly sowing Beijing No. 3 seeds in plastic pots (
Figure G2008102293285D0000031
Figure G2008102293285D0000032
H=15cm), and finally set 2 seedlings in each pot. The concentration of cadmium (mg/kg) added is 0, 1, 2.5 and 5. Water regularly to keep the soil moisture at 40-90% of the field capacity. Plant the Chinese cabbage in the cadmium-contaminated soil, the aboveground part of the Chinese cabbage will repel and absorb the cadmium in the polluted soil, and at the same time, the roots will fix and absorb the cadmium, thereby preventing it from transferring to the aboveground part; The aboveground part of the cabbage is harvested from the contaminated soil, the roots are removed and treated properly, and the second crop is planted in the next growth period, and the above operation is repeated. The Chinese cabbage roots after each harvest are specially concentrated and processed, and the processing methods include incineration, extraction and recovery of lead, or harmless landfill after compression.

所述北京新3号大白菜籽的由国家蔬菜工程技术研究中心和北京京研益农科技发展中心联合研制生产。The Beijing New No. 3 Chinese cabbage seed is jointly developed and produced by the National Vegetable Engineering Technology Research Center and Beijing Jingyan Yinong Science and Technology Development Center.

本发明中,北京新3号大白菜为低积累镉的大白菜品种,其植物基因特征和化学特征如下:生育期60-85天,长势较旺,整齐一致,外叶深绿,页面销皱,开展度较小,叶球中桩叠抱,后期壮心速度快,结球紧密,单株净重4公斤左右。抗病毒症,霉腐病,霜霉病,品质好,耐储运,亩产净菜7000-8000公斤。In the present invention, Beijing Xin No. 3 Chinese cabbage is a Chinese cabbage variety with low accumulation of cadmium, and its plant genetic and chemical characteristics are as follows: the growth period is 60-85 days, the growth is vigorous, uniform, the outer leaves are dark green, and the leaves are wrinkled , the degree of expansion is small, the piles of the leaf balls are overlapped, the speed of growth is fast in the later stage, the balls are tight, and the net weight of a single plant is about 4 kg. Anti-viral disease, mold rot, downy mildew, good quality, resistant to storage and transportation, 7000-8000 kg of clean vegetables per mu.

根据镉污染土壤肥力状况,施用促进该大白菜生长的氮、磷和钾肥等。在镉污染土壤中种植的该大白菜采用室外盆栽栽培和野外大田栽培,定期浇水,使土壤含水量保持在田间持水量的40-90%,并注意防治菜青虫、蚜虫和地蛆。According to the fertility status of cadmium-contaminated soil, nitrogen, phosphorus and potassium fertilizers to promote the growth of the Chinese cabbage were applied. The Chinese cabbage planted in the cadmium-contaminated soil adopts outdoor pot cultivation and field cultivation, waters regularly to keep the soil water content at 40-90% of the field water holding capacity, and pays attention to preventing cabbage caterpillars, aphids and ground maggots.

本发明所采用的大白菜北京新3号属于十字花科芸苔属植物,是典型的低温长日照二年生植物,具有很高的营养价值和经济价值。它既可以直接播种于污染土壤,也可以移苗栽培且生长迅速,栽培管理相对于其它农作物更为简便易操作,是一种适应性很强的蔬菜经济作物。实验表明,北京新3号是一种低积累镉的大白菜品种,对土壤中的镉毒害具有很强的耐性、其地上部镉含量低于国家安全蔬菜的生产标准。The Chinese cabbage Beijing Xin No. 3 used in the invention belongs to Brassicaceae Brassica plants, is a typical low-temperature long-sunshine biennial plant, and has high nutritional value and economic value. It can be directly sown in polluted soil, and can also be transplanted and grown rapidly. Compared with other crops, its cultivation management is simpler and easier to operate. It is a highly adaptable vegetable economic crop. Experiments have shown that Beijing Xin No. 3 is a Chinese cabbage variety with low accumulation of cadmium. It has strong tolerance to cadmium poisoning in the soil, and its aboveground cadmium content is lower than the national safety vegetable production standard.

收获的北京新3号可以作为绿色蔬菜供人们食用,也可以在国内市场上出售或者出口国外,从而体现出其较高的食用价值和经济价值;另外,对收获的白菜根可以集中处理,或者安全填埋,或者进行资源化加工处理,回收其中的镉,可以避免二次污染,同时产生一定的经济效益。The harvested Beijing Xin No. 3 can be used as a green vegetable for people to eat, and it can also be sold in the domestic market or exported abroad, thus reflecting its high edible value and economic value; in addition, the harvested cabbage root can be treated centrally, or Safe landfilling, or resource processing and recycling of cadmium, can avoid secondary pollution and produce certain economic benefits at the same time.

本发明的优点及有益效果如下:Advantage of the present invention and beneficial effect are as follows:

1.本发明方法与传统的污染土壤治理方法相比,具有投资少、工作量小、技术要求不高、生态安全性高、不产生二次污染等优点。1. Compared with traditional contaminated soil treatment methods, the method of the present invention has the advantages of less investment, less workload, low technical requirements, high ecological safety, and no secondary pollution.

2.本发明具有良好的经济、环境和生态效益。本发明方法作为一种污染土壤的安全生产技术,所收获大白菜地上部镉含量低于国家相关标准,食用该白菜不会对人体产生危害,可以通过出售该白菜获得较高的经济效益;对大白菜北京新3号根进行集中处理,不会造成二次污染,同时固定修复进程不仅不会破坏土壤生态环境,还有助于改善因重金属污染而引起的土壤退化和生产力下降,恢复并提高其生物多样性。2. The present invention has good economic, environmental and ecological benefits. The method of the present invention is used as a safe production technology for polluted soil. The cadmium content in the aboveground part of the harvested Chinese cabbage is lower than the relevant national standard. Eating the cabbage will not cause harm to the human body, and higher economic benefits can be obtained by selling the cabbage; Chinese cabbage Beijing Xin No. 3 roots are treated intensively, which will not cause secondary pollution. At the same time, the fixed restoration process will not only not damage the soil ecological environment, but also help to improve soil degradation and productivity decline caused by heavy metal pollution, restore and improve its biodiversity.

附图说明Description of drawings

图1为在不同浓度的镉处理下北京新3号的地上部和根部生物量检测图。Figure 1 is the detection graph of shoot and root biomass of Beijing Xin No. 3 treated with different concentrations of cadmium.

具体实施方式Detailed ways

实施例1:盆栽梯度下低积累镉的大白菜的确认与模拟应用Example 1: Confirmation and simulation application of Chinese cabbage with low accumulation of cadmium under pot gradient

盆栽实验地点设在中国科学院沈阳生态站内,该站地处下辽河平原的中心地带,距沈阳市区约35km,属暖温带半湿润大陆性气候。实验场地周围没有污染源,是重金属未污染区。盆栽试验土壤采自中国科学院沈阳生态站内无污染区的表层土壤(0-20cm),土壤类型为草甸棕壤,土壤中重金属镉含量为0.17mg/kg。The potting experiment site is located in the Shenyang Ecological Station of the Chinese Academy of Sciences, which is located in the center of the Lower Liaohe Plain, about 35km away from Shenyang City, and belongs to the warm temperate semi-humid continental climate. There is no pollution source around the experimental site, which is an uncontaminated area of heavy metals. The potting test soil was collected from the surface soil (0-20cm) in the non-polluted area of the Shenyang Ecological Station of the Chinese Academy of Sciences. The soil type was meadow brown soil, and the heavy metal cadmium content in the soil was 0.17mg/kg.

本实验共设4个处理,每个处理重复3次,投加的镉浓度(mg/kg)分别为:0(对照,未投加)、1、2.5、和5。实验投加的镉为分析纯的CdCl2·2.5H2O,并施入一定浓度的氮、磷、钾肥作为底肥:0.25g/kgN(CO(NH2)2)、0.065g/kgP和0.124g/kg K(K2HPO4)。将供试土壤风干过4mm筛后,以固体形式拌入镉和底肥,充分混匀后装入塑料盆(

Figure G2008102293285D0000041
H=15cm)中,每盆装土2.5kg,平衡8-12周待用。将大白菜籽直接播种于盆内,每盆播种3-6粒,待发芽一周后间苗,根据白菜幼苗长势每盆留两株。每日根据盆中土壤水分状况,浇入适量自来水,使土壤含水量经常保持在田间持水量的40-90%左右。In this experiment, 4 treatments were set up, and each treatment was repeated 3 times. The cadmium concentrations (mg/kg) added were: 0 (control, no addition), 1, 2.5, and 5. The cadmium added in the experiment was analytically pure CdCl 2 ·2.5H 2 O, and a certain concentration of nitrogen, phosphorus, and potassium fertilizers were applied as the base fertilizer: 0.25g/kgN(CO(NH 2 ) 2 ), 0.065g/kgP and 0.124 g/kg K (K 2 HPO 4 ). After air-drying the test soil and passing through a 4mm sieve, mix cadmium and base fertilizer in solid form, mix well and put it into a plastic basin (
Figure G2008102293285D0000041
H=15cm), each pot is filled with 2.5kg of soil, and balanced for 8-12 weeks before use. Sow Chinese cabbage seeds directly in pots, sow 3-6 seeds in each pot, and thin out the seedlings after one week of germination, and leave two cabbage seedlings in each pot according to the growth of cabbage seedlings. According to the soil moisture status in the pot, pour an appropriate amount of tap water every day to keep the soil moisture content at about 40-90% of the field water holding capacity.

植物生长60-85天后收获植株。将收获样品分为根和地上部两部分,分别用自来水充分冲洗以去除粘附于植物样品上的泥土和污物,然后再用去离子水冲洗,沥去水分,在105℃杀青1小时;然后在65℃下烘干至衡重,称量干重后粉碎备用。植物样品采用HNO3-HClO4法消化(二者体积比为87∶13),用原子吸收分光光度计测定其中的镉含量。Plants were harvested after 60-85 days of plant growth. Divide the harvested sample into two parts, the root and the shoot, and rinse them with tap water to remove the soil and dirt adhering to the plant sample, then rinse with deionized water, drain the water, and kill at 105°C for 1 hour; Then dry at 65°C to constant weight, weigh the dry weight and pulverize for later use. Plant samples were digested by HNO 3 -HClO 4 method (the volume ratio of the two was 87:13), and the cadmium content was determined by atomic absorption spectrophotometer.

实验结果如下:The experimental results are as follows:

图1给出了在上述不同浓度的Cd处理下北京新3号的地上部和根部生物量。方差分析表明,与对照(Cd 0)相比,在Cd投加浓度为1、2.5和5mg/kg的处理中,北京新3号地上部和根部生物量均未显著下降,表现出很强的耐性,Cd浓度小于2.5mg/kg的处理甚至对其生长有一定的促进作用。但当Cd污染浓度很高的情况下,即Cd处理浓度为5mg/kg时,地上部和根部生物量稍有下降,但是为到显著水平(P>0.05),也表现出了较大的Cd耐性。Figure 1 shows the shoot and root biomass of Beijing Xin 3 under the above-mentioned different concentrations of Cd. Analysis of variance showed that, compared with the control (Cd 0), in the treatment of Cd concentration of 1, 2.5 and 5 mg/kg, the shoot and root biomass of Beijing Xin No. Tolerance, the treatment with Cd concentration less than 2.5mg/kg even has a certain promoting effect on its growth. However, when the concentration of Cd pollution was very high, that is, when the concentration of Cd was 5 mg/kg, the biomass of shoots and roots decreased slightly, but at a significant level (P>0.05), it also showed a large amount of Cd patience.

参见表1可知,随着土壤中投加Cd浓度的增加,北京新3号地上部和根部的Cd浓度逐步升高,其富集系数和转移系数也有升高的趋势。但在所有的Cd处理中,北京新3号地上部Cd富集系数(地上部和土壤中Cd含量的比值)和转移系数(地上部与根部Cd含量的比值)始终小于1,即地上部Cd含量始终低于土壤中和根部的Cd含量,具备了典型的Cd排异植物特征。本次试验测得白菜北京新3号地上部平均含水量为90%。而农产品安全质量无公害蔬菜安全要求(GB18406.1-2001)Cd最大容许含量(MPC)为0.05mg/kg鲜重(FW),白菜地上部镉含量超过0.50mg/kg干重(DW)即认为Cd超标。根据这一标准,当土壤Cd浓度<2.5mg/kg时,北京新3号地上部Cd含量都小于0.50mg/kg,满足国家无公害蔬菜的生产要求。当土壤Cd浓度≥2.5mg/kg时,北京新3号地上部Cd含量超过了国家标准。Referring to Table 1, it can be seen that with the increase of Cd concentration in the soil, the Cd concentration in the aboveground and roots of Beijing Xin 3 gradually increased, and its enrichment coefficient and transfer coefficient also tended to increase. However, in all Cd treatments, the aboveground Cd enrichment coefficient (ratio of aboveground and soil Cd content) and transfer coefficient (ratio of aboveground and root Cd content) of Beijing Xin No. 3 were always less than 1, that is, aboveground Cd The content is always lower than the Cd content in the soil and roots, which has the typical characteristics of Cd rejection plants. The average water content of the aboveground part of cabbage Beijing Xin No. 3 was measured in this experiment to be 90%. The maximum permissible content (MPC) of Cd in the safety and quality of agricultural products and pollution-free vegetables (GB18406.1-2001) is 0.05 mg/kg fresh weight (FW), and the cadmium content in the aboveground part of cabbage exceeds 0.50 mg/kg dry weight (DW). It is considered that Cd exceeds the standard. According to this standard, when the soil Cd concentration is less than 2.5mg/kg, the aboveground Cd content of Beijing Xin No. 3 is less than 0.50mg/kg, which meets the national pollution-free vegetable production requirements. When the soil Cd concentration was ≥2.5mg/kg, the aboveground Cd content of Beijing Xin 3 exceeded the national standard.

上述实验结果表明,在土壤中Cd投加浓度低于2.5mg/kg时,北京新3号地上部镉含量始终低于国家无公害蔬菜的生产要求的标准,而且地上部Cd含量始终低于根部和土壤Cd含量,同时对Cd污染表现出较强的耐性,完全具备了Cd低富集植物的特征,是一种Cd低富集植物。并且,其根部Cd含量都大于土壤中Cd含量,表现出较强的对Cd的吸收固定作用。北京新3号对Cd有很强的耐性、地上部排斥吸收Cd、根部固定吸附Cd,并且阻止其上地上部转运,这些特征使得它在低度Cd污染土壤安全生产,并逐步去除土壤中过量的Cd成为可能。The above experimental results show that when the concentration of Cd in the soil is lower than 2.5mg/kg, the cadmium content in the aboveground part of Beijing Xin No. 3 is always lower than the national standard for the production of pollution-free vegetables, and the Cd content in the aboveground part is always lower than that in the roots. At the same time, it shows strong tolerance to Cd pollution, fully possesses the characteristics of low Cd enrichment plants, and is a low Cd enrichment plant. Moreover, the Cd content in the roots is greater than that in the soil, showing a strong absorption and fixation effect on Cd. Beijing Xin No. 3 has a strong tolerance to Cd, its shoots repel and absorb Cd, its roots fix and absorb Cd, and prevent its translocation in the shoots. These characteristics make it safe to produce in low-level Cd-polluted soils and gradually remove excess Cd made possible.

表1盆栽实验条件下北京新3号对Cd的低积累特征Table 1 The characteristics of low accumulation of Cd in Beijing Xin 3 under pot experiment conditions

  处理浓度(mg/kg)Treatment concentration (mg/kg)   地上部(mg/kg)Aerial parts (mg/kg)   根部(mg/kg)Root (mg/kg)   地上部富集系数Aerial enrichment factor 转移系数transfer coefficient   00   0.080.08   0.380.38   0.210.21   1 1   0.220.22   1.961.96   0.220.22   0.110.11   2.52.5   0.900.90   4.254.25   0.360.36   0.230.23   55   3.153.15   8.628.62   0.630.63   0.370.37

实施例2:沈抚灌区大田确认与镉污染土壤的安全生产Example 2: Field confirmation in Shenfu irrigation area and safe production of cadmium-contaminated soil

实验地点设在沈阳市东陵区的沈抚灌区试验田进行,该地的气象条件与沈阳生态站比较相差不大。土壤类型为黄棕壤,其理化性质为pH值8.5,有机质18.32mg/kg,全N 1.27mg/kg,全P 1.42mg/kg,速效K 145.68mg/kg,总镉1.25mg/kg。将大白菜籽直接穴播于镉污染土壤中,每穴3-6粒白菜籽,穴间距10-60cm,垄间距10-60cm。根据土壤水分丰缺状况,不定期用井水浇水(水中未检出Cd),使土壤含水量经常保持在田间持水量的40-90%左右;为促进丰源生长,按照300kg/公顷的用量施入尿素。待生长60-85天以后收获北京新3号,样品处理同实施例1。The experiment site is set in the experimental field of Shenfu Irrigation Area in Dongling District, Shenyang City, where the meteorological conditions are not much different from those of the Shenyang Ecological Station. The soil type is yellow brown soil, its physical and chemical properties are pH 8.5, organic matter 18.32mg/kg, total N 1.27mg/kg, total P 1.42mg/kg, available K 145.68mg/kg, total cadmium 1.25mg/kg. Sow Chinese cabbage seeds directly in the cadmium-contaminated soil, with 3-6 cabbage seeds per hole, the hole spacing is 10-60cm, and the ridge spacing is 10-60cm. According to the abundance and shortage of soil moisture, water from wells from time to time (Cd is not detected in the water), so that the soil moisture is often kept at about 40-90% of the field water holding capacity; Dosage of urea. Harvest Beijing Xin No. 3 after 60-85 days of growth, and the sample treatment is the same as in Example 1.

实验结果如下:The experimental results are as follows:

表2给出了不同Cd浓度小区北京新3号对Cd低累积特征和生长情况。北京新3号地上部含量均低于0.50mg/kg,符合国家无公害安全蔬菜的标准(GB18406.1-2001),其地上部Cd含量远低于根部,且富集系数均小于1,可作为Cd低积累大白菜在中轻度污染的农田土壤中推广种植,而不会对人体健康造成危害。按照测定的根部平均干重折算,在Cd 1.25mg/kg的污染水平下,北京新3号的根的干物质产量可达9.05kg/公顷,从土壤中带走的镉为16.29mg/公顷从而通过北京新3号的不断生产可以在一定程度上去除了土壤中过量的Cd。由此可知,利用北京新3号在轻度Cd(镉含量为0.5-2.5mg/kg)污染的土壤上,边安全生产、边修复土壤镉污染是现实可行的。Table 2 shows the low Cd accumulation characteristics and growth conditions of Beijing Xin 3 plots with different Cd concentrations. The aboveground content of Beijing Xin No. 3 is all lower than 0.50mg/kg, which meets the national standard for pollution-free and safe vegetables (GB18406.1-2001). As a low Cd accumulation Chinese cabbage, it can be popularized and planted in moderately and lightly polluted farmland soil without causing harm to human health. Converted according to the measured root average dry weight, under the pollution level of Cd 1.25mg/kg, the root dry matter yield of Beijing Xin 3 can reach 9.05kg/ha, and the cadmium taken away from the soil is 16.29mg/ha. The excessive Cd in the soil can be removed to some extent by the continuous production of Beijing Xin 3. It can be seen from this that it is realistic and feasible to use Beijing Xin No. 3 to repair soil cadmium pollution at the same time of safe production and soil cadmium pollution on mildly Cd (cadmium content of 0.5-2.5mg/kg) polluted soil.

表2大田实验条件下北京新3号对Cd的低累积特征和生长状况Table 2 Low Cd accumulation characteristics and growth status of Beijing Xin 3 under field experiment conditions

白菜株Cabbage strain   地上部含Cd(mg/kg)Containing Cd in the aerial part (mg/kg)   根部含Cd(mg/kg)Root contains Cd (mg/kg)   地上部富集系数Aerial enrichment factor   地上部干重(g/株)Dry weight of aerial parts (g/plant)   根部干重(g/株)Root dry weight (g/plant)   1 1   0.230.23   1.881.88   0.180.18   58.7258.72   11.7611.76   2 2   0.270.27   1.691.69   0.220.22   66.9666.96   12.4312.43   33   0.340.34   2.232.23   0.270.27   57.1357.13   15.2915.29   44   0.350.35   2.122.12   0.270.27   63.2263.22   14.3614.36   55   0.260.26   1.651.65   0.200.20   68.3368.33   11.2311.23   66   0.220.22   1.991.99   0.180.18   65.2465.24   16.4516.45   77   0.330.33   2.082.08   0.270.27   57.1557.15   17.0417.04   8 8   0.350.35   1.781.78   0.270.27   65.8265.82   10.8810.88   9 9   0.410.41   2.132.13   0.290.29   61.2961.29   13.4113.41   1010   0.390.39   2.452.45   0.280.28   66.1566.15   18.4418.44

实施例结果表明,本发明利用低积累Cd大白菜地上部排斥吸收富集污染土壤中的Cd,根部固定吸附土壤中的Cd,从而阻止其向上转运到地上部,当大白菜生长到60-85天时将大白菜整体收获,地上部作为安全蔬菜食用或出售,根部给予其他妥善处理,从而即可以保障蔬菜食品安全,又可以利用非食用部位(根部)固定吸附土壤中大量的Cd;通过反复种植该种蔬菜作物,重复上述过程,就可以连续提取污染土壤中过量的Cd,并且大白菜可食部位的Cd含量也满足无公害安全蔬菜的标准。该方法具有费用低,不破坏土壤的理化性质,无二次污染,且具有在污染土壤中边生产、边治理等优点。The results of the examples show that the present invention utilizes low-accumulation Cd aboveground parts of Chinese cabbage to repel and absorb Cd in the enriched polluted soil, and the roots fix and absorb Cd in the soil, thereby preventing it from being transported upwards to the aboveground parts. When the Chinese cabbage grows to 60-85 Tianshi harvests Chinese cabbage as a whole, the aboveground part is eaten or sold as a safe vegetable, and the root part is given other proper treatment, so that the food safety of vegetables can be guaranteed, and a large amount of Cd in the soil can be fixed and absorbed by the non-edible part (root part); through repeated planting For this kind of vegetable crop, by repeating the above process, excess Cd in the polluted soil can be continuously extracted, and the Cd content in the edible parts of Chinese cabbage also meets the standard of pollution-free and safe vegetables. The method has the advantages of low cost, no damage to the physical and chemical properties of the soil, no secondary pollution, and the advantages of simultaneous production and simultaneous treatment in the polluted soil.

Claims (6)

1.一种利用镉低积累大白菜北京新3号农业安全生产的方法,其特征在于:在镉污染土壤中种植低积累镉的大白菜北京新3号,边生产边修复,实现去除土壤中过量镉的目的。1. A method for safe agricultural production of Beijing No. 3 Chinese cabbage with low accumulation of cadmium, characterized in that: planting No. 3 Chinese cabbage Beijing New with low accumulation of cadmium in cadmium-contaminated soil, repairing while producing, and realizing removal of the cadmium in the soil Purpose of excess cadmium. 2.按照权利要求1所述的利用镉低积累大白菜北京新3号农业安全生产的方法,其特征在于:所述在镉污染土壤中种植的大白菜为北京新3号,该大白菜为低积累重金属镉的蔬菜作物。2. according to claim 1, utilize the method for safe agricultural production of Beijing No. 3 Chinese cabbage with low accumulation of cadmium, characterized in that: the Chinese cabbage planted in the cadmium-contaminated soil is Beijing No. 3, and the Chinese cabbage is Vegetable crops with low accumulation of heavy metal cadmium. 3.按照权利要求1所述的利用镉低积累大白菜北京新3号农业安全生产的方法,其特征在于:所述在镉污染土壤中种植该大白菜,是指将该品种大白菜籽直接穴播于镉污染土壤中,每穴3-6粒白菜籽,穴间距10-60cm,垄间距10-60cm。3. according to claim 1, utilize the method for safe agricultural production of Beijing No. 3 Chinese cabbage with low accumulation of cadmium, characterized in that: said planting the Chinese cabbage in the cadmium-contaminated soil refers to the variety of Chinese cabbage seeds directly Hole sowing in cadmium-contaminated soil, 3-6 cabbage seeds per hole, hole spacing 10-60cm, ridge spacing 10-60cm. 4.按照权利要求1所述的利用镉低积累大白菜北京新3号实施农产品安全生产的方法,其特征在于:在镉污染土壤中种植的该大白菜,采用室外盆栽栽培和野外大田栽培,定期浇水,使土壤含水量保持在田间持水量的40-90%。4. according to the method for implementing safe production of agricultural products by using the Chinese cabbage Beijing Xin No. 3 with low accumulation of cadmium according to claim 1, it is characterized in that: the Chinese cabbage planted in cadmium-contaminated soil adopts outdoor pot cultivation and field field cultivation, Water regularly to keep the soil moisture at 40-90% of the field capacity. 5.按照权利要求1所述的利用镉低积累大白菜北京新3号实施农产品安全生产的方法,其特征在于:在镉污染土壤中种植该大白菜,该大白菜地上部排斥吸收污染土壤中镉,同时根部固定、吸附镉,从而阻止其向地上部转移;当大白菜生长60-85天成熟时,将大白菜地上部从污染土壤上收获,将根部移除后妥善处理,待下个生长期再种植第二茬,重复上述操作。5. The method for implementing safe production of agricultural products by utilizing Chinese cabbage with low cadmium accumulation Beijing No. 3 according to claim 1, characterized in that: the Chinese cabbage is planted in cadmium-contaminated soil, and the upper part of the Chinese cabbage repells and absorbs the polluted soil At the same time, the roots fix and absorb cadmium, thereby preventing its transfer to the above-ground parts; when the Chinese cabbage grows for 60-85 days and matures, the above-ground parts of the Chinese cabbage are harvested from the polluted soil, and the roots are removed and disposed of properly, until the next day. During the growth period, plant the second crop and repeat the above operation. 6.按权利要求1所述的利用镉低积累大白菜北京新3号农业安全生产的方法,其特征在于:所述的每次收割后的大白菜根进行专门集中处理,处理的方法包括焚烧、萃取回收铅或压缩后作无害化填埋。6. The method for safe production of Chinese cabbage Beijing No. 3 with low cadmium accumulation according to claim 1, characterized in that: the Chinese cabbage roots after each harvest are specially concentrated and processed, and the processing methods include burning, Extract and recover lead or compress it for harmless landfill.
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Publication number Priority date Publication date Assignee Title
CN103109651A (en) * 2011-11-16 2013-05-22 中国科学院沈阳应用生态研究所 Method of producing while recovering for cadmium-polluted vegetable field
CN103250539A (en) * 2013-05-16 2013-08-21 农业部环境保护科研监测所 Method for reducing absorption of cadmium by rape
CN107889840A (en) * 2017-11-15 2018-04-10 陕西理工大学 A kind of hardening agent for strengthening super enriching plant cadmium pollution soil repair and its application
CN109622593A (en) * 2018-12-06 2019-04-16 钦州学院 A kind of biological renovation method of cadmium pollution soil
CN110496852A (en) * 2018-05-16 2019-11-26 沈阳药科大学 A method for remediating cadmium-contaminated soil
CN110639947A (en) * 2019-09-27 2020-01-03 中国计量大学 A test method to verify the reduction of cadmium absorption by cabbage
CN113600609A (en) * 2021-07-30 2021-11-05 山东省农业科学院 Crop restoration method for reducing cadmium content in soil

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103109651A (en) * 2011-11-16 2013-05-22 中国科学院沈阳应用生态研究所 Method of producing while recovering for cadmium-polluted vegetable field
CN103250539A (en) * 2013-05-16 2013-08-21 农业部环境保护科研监测所 Method for reducing absorption of cadmium by rape
CN107889840A (en) * 2017-11-15 2018-04-10 陕西理工大学 A kind of hardening agent for strengthening super enriching plant cadmium pollution soil repair and its application
CN107889840B (en) * 2017-11-15 2020-06-16 陕西理工大学 Enhancer for enhancing hyperaccumulation plants to restore cadmium-contaminated soil and application thereof
CN110496852A (en) * 2018-05-16 2019-11-26 沈阳药科大学 A method for remediating cadmium-contaminated soil
CN109622593A (en) * 2018-12-06 2019-04-16 钦州学院 A kind of biological renovation method of cadmium pollution soil
CN110639947A (en) * 2019-09-27 2020-01-03 中国计量大学 A test method to verify the reduction of cadmium absorption by cabbage
CN113600609A (en) * 2021-07-30 2021-11-05 山东省农业科学院 Crop restoration method for reducing cadmium content in soil

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