CN101693877A - Stereoscopic cell culturing carrier - Google Patents
Stereoscopic cell culturing carrier Download PDFInfo
- Publication number
- CN101693877A CN101693877A CN200910055906A CN200910055906A CN101693877A CN 101693877 A CN101693877 A CN 101693877A CN 200910055906 A CN200910055906 A CN 200910055906A CN 200910055906 A CN200910055906 A CN 200910055906A CN 101693877 A CN101693877 A CN 101693877A
- Authority
- CN
- China
- Prior art keywords
- cell culturing
- stereoscopic
- bar
- stereoscopic cell
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012258 culturing Methods 0.000 title claims abstract description 33
- -1 polypropylene Polymers 0.000 claims description 12
- 239000004793 Polystyrene Substances 0.000 claims description 7
- 229920002223 polystyrene Polymers 0.000 claims description 7
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 239000004743 Polypropylene Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 229920001155 polypropylene Polymers 0.000 claims description 6
- 150000001408 amides Chemical class 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 239000000969 carrier Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a stereoscopic cell culturing carrier, comprising a culturing box, wherein the culturing box is provided with a stereoscopic culturing bracket inside; the stereoscopic culturing bracket comprises more than two plane structure layers; the plane structure layers are coincided along the direction vertical to the plane direction; any one of the plane structure layers comprises at least more than two rail with parallel intervals; and the plane structure layers are fixed through more than two parallel straight bars. The plane structure layers are formed by adopting the rails with parallel intervals, pores in vertical direction are formed by utilizing more than two coincided plane structure layers, therefore, the cells can grow along the plane direction and the vertical direction. The straight bars or the rails have oval radial cross sections, or triangular round radial cross sections, or fan-shaped radial cross sections, or crescent radial cross sections or polygon radial cross sections, and can enlarge the porous internal surface area, thereby being more beneficial to the cell growing.
Description
Technical field:
The present invention relates to biological field, relate in particular to cell culture apparatus, particularly stereoscopic cell culturing carrier.
Background technology:
In biotechnology, often need culturing cell.The carrier of cell cultures extensively adopts polystyrene plate, and cell is planar grown.But, the planar growth mode of cell is different with the stereoscopic three-dimensional growth pattern in the actual live body, may aim at and confirm know cell, the biochemistry of tissue, pathology characteristic and have a negative impact, and cell in cultivate on the plane growth slowly, be unfavorable for finishing rapidly of Bioexperiment.
Summary of the invention:
The object of the present invention is to provide stereoscopic cell culturing carrier, described this stereoscopic cell culturing carrier will solve in the prior art cell planar growth mode and aim at and confirm to know cell, the biochemistry of tissue, the technical problem that the pathology characteristic has a negative impact, to solve simultaneously cell in cultivate on the plane growth slowly, be unfavorable for the technical problem of finishing rapidly of Bioexperiment.
Stereoscopic cell culturing carrier of the present invention, cultivating box by one constitutes, be provided with a stereoscopic cell culturing bracket in the described cultivation box, described stereoscopic cell culturing bracket is made of plural two dimensional structure layer, described two dimensional structure layer is along superimposed perpendicular to its planar direction, any one described two dimensional structure layer all is made of the parallel cross bar parallel interval of two above numbers at least, described two dimensional structure layer is fixed by the parallel straight-bar of two above numbers, and the angle between any one described parallel straight-bar and the horizontal plane is spent smaller or equal to 180 more than or equal to 0 degree.
Further, described parallel straight-bar is arranged.
Further, the radial section ovalize of described parallel straight-bar or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
Further, the radial section ovalize of described cross bar or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
Further, include 5 limits in the described Polygons at least, described limit is straight line or curve.
Further, described cross bar adopts polystyrene or polypropylene or polyethylene or polycarbonate or polymeric amide manufacturing with parallel straight-bar.
Further, described cultivation box comprises one deck culture plate at least, is provided with at least one culture tank in the described culture plate.
Further, be provided with at least one blind hole in the described cultivation box, described blind hole is provided with blank cap.
Further, described cultivation box adopts polystyrene or polypropylene or polyethylene manufacturing.
Principle of work of the present invention is: in any one two dimensional structure layer, be provided with the gap between two adjacent arbitrarily cross bars, this gap can be for cell along planar growth.The two dimensional structure of two above numbers is stacked add after, neighbouring cross bar intersects to form the gap, this gap can supply the vertically three-dimensional growth of cell.Have ellipse or rhizoma sparganic circle or straight-bar or cross bar fan-shaped or crescent or Polygons radial section, can expand the internal surface area in formed hole, gap, thereby more help the cell growth.
The present invention and prior art compare, and its effect is actively with tangible.The present invention utilizes the cross bar of parallel interval to constitute the two dimensional structure layer, utilizes the stacked hole that adds back formation vertical direction of two dimensional structure of two above numbers, and cell can be along plane and vertical direction growth.Have ellipse or rhizoma sparganic circle or straight-bar or cross bar fan-shaped or crescent or Polygons radial section, can expand the internal surface area of hole, thereby more help the cell growth.
Description of drawings:
Fig. 1 is a side-looking structural representation of stereoscopic cell culturing carrier of the present invention.
Fig. 2 is another side-looking structural representation of stereoscopic cell culturing carrier of the present invention.
Fig. 3 is a plan structure synoptic diagram of stereoscopic cell culturing carrier of the present invention.
Fig. 4 is a structural representation of stereoscopic cell culturing carrier of the present invention.
Embodiment:
As Fig. 1, Fig. 2, Fig. 3 and shown in Figure 4, stereoscopic cell culturing carrier of the present invention, constitute by a cultivation box 4 with cover, be provided with a stereoscopic cell culturing bracket in the described cultivation box 4, described stereoscopic cell culturing bracket is made of plural two dimensional structure layer 1, described two dimensional structure layer 1 is along superimposed perpendicular to its planar direction, any one described two dimensional structure layer 1 all is made of parallel cross bar 2 parallel interval of two above numbers at least, described two dimensional structure layer 1 is fixed by plural parallel straight-bar 3, and the angle between any one described straight-bar 3 and the horizontal plane is spent smaller or equal to 180 more than or equal to 0 degree.
Further, described straight-bar 3 is arranged.
Further, the radial section ovalize of described straight-bar 3 or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
Further, the radial section ovalize of described cross bar 2 or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
Further, include 5 limits in the described Polygons at least, described limit is straight line or curve.
Further, described cross bar 2 and straight-bar 3 adopt polystyrene or polypropylene or polyethylene or polycarbonate or polymeric amide manufacturing.
Further, described cultivation box 4 with cover comprises one deck culture plate 5 at least, is provided with at least one culture tank 6 in the described culture plate 5.
Further, be provided with at least one blind hole in the described cultivation box 4, described blind hole is provided with blank cap.
Further, described stereoscopic cell culturing carrier adopts polystyrene or polypropylene or polyethylene manufacturing.
Claims (9)
1. stereoscopic cell culturing carrier, cultivating box by one constitutes, it is characterized in that: be provided with a stereoscopic cell culturing bracket in the described cultivation box, described stereoscopic cell culturing bracket is made of plural two dimensional structure layer, described two dimensional structure layer is along superimposed perpendicular to its planar direction, any one described two dimensional structure layer all is made of the cross bar parallel interval of two above numbers at least, described two dimensional structure layer is fixed by the parallel straight-bar of two above numbers, and the angle between any one described parallel straight-bar and the horizontal plane is spent smaller or equal to 180 more than or equal to 0 degree.
2. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: described parallel straight-bar is arranged.
3. stereoscopic cell culturing carrier as claimed in claim 1, it is characterized in that: the radial section ovalize of described parallel straight-bar or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
4. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: the radial section ovalize of described cross bar or rhizoma sparganic circle or fan-shaped or crescent or Polygons include 5 limits at least in the described Polygons.
5. as claim 3 or 4 described stereoscopic cell culturing carriers, it is characterized in that: include 5 limits in the described Polygons at least, described limit is straight line or curve.
6. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: described cross bar adopts polystyrene or polypropylene or polyethylene or polycarbonate or polymeric amide manufacturing with parallel straight-bar.
7. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: described cultivation box comprises one deck culture plate at least, is provided with at least one culture tank in the described culture plate.
8. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: be provided with at least one blind hole in the described cultivation box, described blind hole is provided with blank cap.
9. stereoscopic cell culturing carrier as claimed in claim 1 is characterized in that: described cultivation box adopts polystyrene or polypropylene or polyethylene manufacturing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910055906A CN101693877A (en) | 2009-08-04 | 2009-08-04 | Stereoscopic cell culturing carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910055906A CN101693877A (en) | 2009-08-04 | 2009-08-04 | Stereoscopic cell culturing carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101693877A true CN101693877A (en) | 2010-04-14 |
Family
ID=42092879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910055906A Pending CN101693877A (en) | 2009-08-04 | 2009-08-04 | Stereoscopic cell culturing carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101693877A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385596A (en) * | 2012-09-06 | 2016-03-09 | 普拉里斯坦有限公司 | Devices and methods for culture of cells |
-
2009
- 2009-08-04 CN CN200910055906A patent/CN101693877A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385596A (en) * | 2012-09-06 | 2016-03-09 | 普拉里斯坦有限公司 | Devices and methods for culture of cells |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100414 |