CN101693252A - Application of siegesbeckia herb in remediation of soil contaminated with heavy metal cadmium - Google Patents
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Abstract
本发明公开了一种菊科植物豨莶在修复土壤重金属镉污染中的应用,将豨莶种植于重金属镉污染的土壤中即可。利用豨莶对重金属镉的富集特性,将其种植于污染土壤中,可吸收积累重金属镉,并将其大部分转运到地上部分,以达到修复土壤重金属镉污染的目的。在其生长过程中不需要进行特殊管理,因而成本较低、可操作性强;而且,豨莶个体生物量大,可提升单位面积土壤重金属的修复效果,为重金属污染土壤的植物修复开发了新的植物资源品种。
The invention discloses an application of a compositae plant scutellaria in repairing soil polluted by heavy metal cadmium. The snail can only be planted in the soil polluted by heavy metal cadmium. Utilizing the enrichment characteristics of heavy metal cadmium in C. chinensis, planting it in polluted soil can absorb and accumulate heavy metal cadmium, and transfer most of it to the aboveground part, so as to achieve the purpose of remediating soil heavy metal cadmium pollution. It does not require special management during its growth process, so the cost is low and the operability is strong; moreover, the individual biomass of S. chinensis is large, which can improve the effect of remediation of heavy metals in soil per unit area, and has developed a new method for phytoremediation of heavy metal-contaminated soils. species of plant resources.
Description
技术领域technical field
本发明涉及环境中重金属污染的修复技术领域,尤其是一种植物豨莶在修复土壤重金属镉污染中的应用。The invention relates to the technical field of remediation of heavy metal pollution in the environment, in particular to the application of a plant scutellaria in remediation of heavy metal cadmium pollution in soil.
背景技术Background technique
环境中重金属镉(Cd)污染具有隐蔽性、长期性和不可逆性等特点,其污染物在介质中移动性差、滞留时间长、不能被微生物降解,还可经食物链传递等途径最终将影响人类健康,造成不可估量的影响。震惊世界的日本骨痛病事件就是由土壤中的镉元素污染造成的。因此,镉污染土壤急需治理。The heavy metal cadmium (Cd) pollution in the environment has the characteristics of concealment, long-term and irreversibility. Its pollutants have poor mobility in the medium, long residence time, cannot be degraded by microorganisms, and can be transmitted through the food chain, which will eventually affect human health. have an immeasurable impact. The Japanese bone disease incident that shocked the world was caused by the pollution of cadmium in the soil. Therefore, cadmium-contaminated soil urgently needs to be treated.
利用重金属富集植物或超富集植物(hyperaccumulator)进行植物修复是当今环境科学的研究热点之一,它具有投资少、效率高,且不造成二次污染等多种特性,在修复重金属土壤污染中显示了极大的潜力。富集植物或超积累植物大都以群落方式聚集于野外,主要在矿山区、成矿作用带、富含金属元素岩石风化而成土壤上。因此,对废弃的矿山或金属矿露头上自然生长的植物进行调查是寻找富集植物或超积累植物的有效途径之一。Phytoremediation using heavy metal-enriched plants or hyperaccumulators is one of the research hotspots in environmental science today. It has many characteristics such as low investment, high efficiency, and no secondary pollution. shows great potential. Most of the enrichment plants or hyperaccumulation plants gather in the wild in the form of communities, mainly in mining areas, mineralization belts, and soils formed by weathering of rocks rich in metal elements. Therefore, the survey of plants growing naturally on abandoned mines or metal mine outcrops is one of the effective ways to find enriching plants or hyperaccumulating plants.
目前发现的镉超积累植物比较少见,包括遏蓝菜属的少数几种植物;镉的富集植物已发现的有香根草、苎麻、龙葵、小白酒、狼把草等,它们对镉单一及复合污染均有较强的耐性和积累能力。但是目前发现的大多数镉富集植物生长缓慢、植株矮小、地上部生物量小等,在实际应用中受到限制。因此,寻找其他镉富集植物以克服现有修复植物生长缓慢、植株矮小的缺点,培育出生长迅速、植株高、生物量大的品种,是镉污染土壤植物修复技术亟待解决的问题。The cadmium hyperaccumulator plants discovered so far are relatively rare, including a few plants of the genus Brassica genus; the cadmium-enriching plants have been found to include vetiver, ramie, nightshade, small white wine, wolfberry, etc., which are highly resistant to cadmium. Both single and combined pollution have strong tolerance and accumulation ability. However, most of the cadmium-enriched plants found so far grow slowly, the plants are short, and the aboveground biomass is small, which are limited in practical application. Therefore, to find other cadmium-enriched plants to overcome the shortcomings of slow growth and short plants of the existing restoration plants, and to cultivate varieties with rapid growth, tall plants and large biomass is an urgent problem to be solved in the phytoremediation technology of cadmium-contaminated soil.
与此同时,随着工农业生产的发展,土壤镉污染呈加重的态势,尤其是农业土壤镉污染状况令人担忧。而目前可用于镉污染土壤修复的植物品种资源难以满足实际需要,急待进一步研究和扩大可用资源。另外,地球上植物资源相当丰富,要确定哪些植物是否对镉具有相应的富集特性,需要花费大量人力、物力和财力,也未必就能筛选出符合需要的植物品种,从而制约了植物修复的效率及应用。At the same time, with the development of industrial and agricultural production, soil cadmium pollution is increasing, especially the situation of cadmium pollution in agricultural soil is worrying. However, the plant species resources currently available for cadmium-contaminated soil remediation cannot meet the actual needs, and further research and expansion of available resources are urgently needed. In addition, there are abundant plant resources on the earth. It takes a lot of manpower, material and financial resources to determine which plants have the corresponding enrichment characteristics for cadmium. efficiency and application.
豨莶(拉丁名为Siegesbeckia orientalis L.)为菊科豨莶属草本植物,主产于湖南、福建、江苏、浙江、湖北、四川等地。其全草具有祛除风湿,强健筋骨,清热解毒等功效。Siegesbeckia orientalis L. (Latin name Siegesbeckia orientalis L.) is a herbaceous plant of the genus Siegesbeckia in the Asteraceae family. It is mainly produced in Hunan, Fujian, Jiangsu, Zhejiang, Hubei, Sichuan and other places. Its whole plant has the effects of dispelling rheumatism, strengthening muscles and bones, clearing heat and detoxifying.
发明内容Contents of the invention
发明人在从事重金属污染修复的研究工作中,继发现秃疮花、野茼蒿等重金属富集植物之后,再次发现:菊科豨莶属植物豨莶(拉丁名为Siegesbeckia orientalis L.),对土壤环境中的镉重金属具有显著的富集特性。因此,本发明的目的就是针对上述可用于土壤重金属污染生物修复的植物品种资源不足的问题,提供一种新的植物品种豨莶在修复土壤重金属镉污染中的应用。In the research work on the restoration of heavy metal pollution, the inventor discovered again after discovering heavy metal-enriching plants such as bald sore flower and chrysanthemum chrysanthemum: Siegesbeckia orientalis L. Cadmium heavy metals in the environment have significant enrichment properties. Therefore, the object of the present invention is to address the above-mentioned problem of insufficient resources of plant species that can be used for bioremediation of heavy metal pollution in soil, and to provide a new plant variety, Scutellaria chinensis, for remediation of heavy metal cadmium pollution in soil.
为了实现上述发明目的,本发明采用的技术方案如下:In order to realize the foregoing invention object, the technical scheme that the present invention adopts is as follows:
豨莶在修复土壤镉污染中的应用,将豨莶(Siegesbeckia orientalis L.)种植于重金属镉污染的土壤中即可。通过豨莶根系大量吸收污染土壤中的Cd,并将其转移至茎和叶等地上部器官,从而实现除去土壤中污染物重金属Cd的目的。For the application of Siegesbeckia orientalis L. in remediating soil cadmium pollution, it is enough to plant Siegesbeckia orientalis L. in soil polluted by heavy metal cadmium. The Cd in the polluted soil is absorbed by the roots of C. chinensis and transferred to the above-ground organs such as stems and leaves, so as to achieve the purpose of removing the pollutant heavy metal Cd in the soil.
豨莶的生命力非常旺盛,易于成活,可进行直接移栽或将种子直播生长;在自然条件下即可存活,在其整个生长过程中不需要进行特殊管理。Seaweed has very strong vitality and is easy to survive. It can be directly transplanted or grown directly from seeds; it can survive under natural conditions and does not need special management during its entire growth process.
待地上部分形成一定生物量时可进行定期收割,重金属Cd随着地上部分的收割被大量除去。其种子自然繁殖生长快速,留茬部分也可继续生长,不需每年栽种,能有效降低污染土壤中Cd的含量,从而修复污染土壤。Periodic harvesting can be carried out when the aerial parts form a certain amount of biomass, and the heavy metal Cd will be removed in large quantities with the harvesting of the aerial parts. Its seeds naturally reproduce and grow quickly, and the stubble part can continue to grow without the need for annual planting, which can effectively reduce the content of Cd in the polluted soil, thereby remediating the polluted soil.
本发明中的豨莶(Siegesbeckia orientalis L.),为菊科豨莶属一年生草本植物,经试验研究,证明其主要对镉(Cd)重金属具有富集特性。本发明充分利用了豨莶对Cd重金属的富集特性,将其幼苗移栽或将其种子直播至被重金属镉污染的土壤中,吸收积累污染土壤中的Cd重金属,并将其大部分转运到地上部分,降低土壤中Cd的含量,待地上部分形成一定生物量时,可留茬收割进行连续提取,从而达到减少污染土壤中Cd含量的目的。Siegesbeckia orientalis L. in the present invention is an annual herbaceous plant of the genus Siegesbeckia orientalis in the family Asteraceae. Experimental research has proved that it mainly has the characteristic of enriching cadmium (Cd) heavy metals. The present invention makes full use of the enrichment characteristics of Cd heavy metals of S. chinensis, transplants its seedlings or broadcasts its seeds directly into the soil polluted by heavy metal cadmium, absorbs and accumulates Cd heavy metals in the polluted soil, and transfers most of them to For the aboveground part, reduce the Cd content in the soil. When the aboveground part forms a certain amount of biomass, it can be harvested for continuous extraction, so as to achieve the purpose of reducing the Cd content in the polluted soil.
与现有技术相比,本发明的有益效果是:Compared with prior art, the beneficial effect of the present invention is:
发明人通过试验研究发现,豨莶对重金属镉具有显著的富集特性。本发明正是利用豨莶对重金属镉的这种富集特性,将其种植于镉污染土壤中,可吸收积累重金属镉,并将其大部分转运到地上部分,以达到修复污染土壤的目的。并且,由于豨莶的生命力非常旺盛,易于成活,在其整个生长过程中均不需要进行特殊管理,因而成本较低、可操作性强;同时豨莶个体生物量大,可提高单位面积土壤重金属修复效率,为重金属污染土壤的植物修复开发了新的植物资源品种。The inventors have found through experimental research that the Chinese clams have significant enrichment properties for the heavy metal cadmium. The present invention just utilizes the enrichment characteristic of the heavy metal cadmium in the scutellaria, planting it in the cadmium-contaminated soil, can absorb and accumulate the heavy metal cadmium, and transfer most of it to the aboveground part, so as to achieve the purpose of repairing the polluted soil. In addition, because the vitality of Xixue is very strong and easy to survive, no special management is required during the whole growth process, so the cost is low and the operability is strong; at the same time, the individual biomass of Xixapia is large, which can increase the concentration of heavy metals in the soil per unit area. Remediation Efficiency, developed a new variety of plant resources for phytoremediation of heavy metal-contaminated soils.
附图说明Description of drawings
图1为实施例中不同Cd浓度处理条件下豨莶地上部生物量。Fig. 1 is the above-ground biomass of C. chinensis under the treatment conditions of different Cd concentrations in the examples.
具体实施方式Detailed ways
下面结合具体实施方式对本发明作进一步的详细描述。The present invention will be further described in detail below in combination with specific embodiments.
但不应将此理解为本发明上述主题的范围仅限于下述实施例。However, it should not be construed that the scope of the above-mentioned subject matter of the present invention is limited to the following examples.
实施例1Example 1
本实施例为豨莶在修复土壤重金属镉污染中的应用:The present embodiment is the application of SiCin in remediation of heavy metal cadmium pollution in soil:
试验地点设在四川农业大学教学科研农场土壤肥料试验站。The test site is located at the Soil and Fertilizer Experiment Station of the Teaching and Research Farm of Sichuan Agricultural University.
采用盆栽模拟土壤重金属污染的方法,将菊科豨莶属植物豨莶(Siegesbeckiaorientalis L.)种植于投加梯度浓度重金属镉污染过的盆栽土壤中,采用移栽方式,将豨莶幼苗移栽于盆栽中生长;85天后进行留茬收割植物的地上部分,测其中重金属Cd的重量百分含量。Using the method of potting to simulate soil heavy metal pollution, Siegesbeckiaorientalis L., a plant of the Asteraceae family Siegesbeckiaorientalis L., was planted in the potting soil polluted with gradient concentrations of heavy metal cadmium, and the seedlings of Siegesbeckiaorientalis were transplanted in the Growing in a potted plant; after 85 days, carry out stubble and harvest the aerial part of the plant, and measure the weight percentage content of heavy metal Cd therein.
试验共设8个处理,分别为CK(不投加Cd)和7个不同投加浓度试验,投加浓度为10mgkg-1(T1)、30mg kg-1(T2)、60mg kg-1(T3)、90mg kg-1(T4)、120mg kg-1(T5)、150mg kg-1(T6)、180mg kg-1(T7),投加的重金属形态为CdCl2·5H2O,为分析纯试剂,以固态加入到土壤中,充分混匀,平衡四周后待用。A total of 8 treatments were set up in the experiment, namely CK (without adding Cd) and 7 experiments with different dosage concentrations, the dosage concentrations were 10mgkg -1 (T1), 30mg kg -1 (T2), 60mg kg -1 (T3 ), 90mg kg -1 (T4), 120mg kg -1 (T5), 150mg kg -1 (T6) , 180mg kg -1 (T7). The reagent is added to the soil in solid form, mixed thoroughly, and balanced for four weeks before use.
本试验于春天开始,移栽豨莶幼苗为采自四川省甘孜州康定县南部矿区的隔代遗传种苗,每盆6棵,3次重复。于网室内栽培,根据土壤水分实际情况不定期浇水确保土壤水分保持在田间持水量的80%左右,待植株成熟后收获测定其中的重金属镉含量。The experiment started in spring, and the seedlings were transplanted from the transgenerational genetic seedlings collected from the southern mining area of Kangding County, Ganzi Prefecture, Sichuan Province. There were 6 plants in each pot and 3 repetitions. Cultivate in a net room, water from time to time according to the actual situation of soil moisture to ensure that the soil moisture is kept at about 80% of the field water holding capacity, and the heavy metal cadmium content is measured after the plants are mature.
试验结果如下:The test results are as follows:
图1为不同Cd浓度处理条件下豨莶地上部生物量(图1中,a、b、c、d是差异显著性标注)。最小显著差数法差异显著性分析表明,Cd投加浓度为10、30、60 mg kg-1的处理中,地上部生物量与对照相比,无显著性差异(P<0.05),表现出较强的耐性。但在更高的Cd污染处理水平下,即投加浓度大于90mg kg-1以后,地上部生物量有所下降(P<0.05),说明豨莶耐性虽然较强但还是有一定限度,在Cd浓度大于90mg kg-1以后,植物的生长会受到抑制。Figure 1 shows the biomass of the aboveground parts of C. chinensis under different Cd concentration treatment conditions (in Figure 1, a, b, c, and d are marked for significant differences). The significance analysis of the least significant difference method showed that there was no significant difference (P<0.05) in the aboveground biomass between the treatments with Cd concentration of 10, 30, and 60 mg kg -1 compared with the control (P<0.05), showing Strong patience. However, at a higher level of Cd pollution treatment, that is, after the concentration was greater than 90 mg kg -1 , the aboveground biomass decreased (P < 0.05), indicating that although the tolerance of Silicia chinensis is strong, it still has a certain limit. When the concentration is greater than 90mg kg -1 , the growth of plants will be inhibited.
植物体内Cd含量测定结果见下述表1:The results of Cd content determination in plants are shown in the following table 1:
表1.不同处理浓度下豨莶对镉的富集特征Table 1. The enrichment characteristics of cadmium in C. chinensis under different treatment concentrations
TF为转移系数,BCF为富集系数TF is transfer coefficient, BCF is enrichment coefficient
表1结果表明,土壤中镉投加浓度在T1~T7浓度时,豨莶地上部富集系数均大于1,转移系数也均大于1,在土壤中投加浓度为60mg kg-1时,植株地上部含量大于100mg kg-1,达到了超富集植物含量的临界标准,且植株体内镉含量随投加浓度的增高而增高。The results in Table 1 show that when the concentration of cadmium in the soil is between T1 and T7, the enrichment coefficients and transfer coefficients of the aboveground parts of C. The shoot content was greater than 100mg kg -1 , which reached the critical standard of hyperaccumulator plant content, and the cadmium content in the plant increased with the increase of the concentration.
综合豨莶对镉的耐性和富集特征来看,豨莶为镉的超富集植物,对Cd污染土壤具有较强的修复能力。Based on the tolerance and enrichment characteristics of Cd to Cd, Cicada is a hyperaccumulator of Cd, and has a strong ability to repair Cd-contaminated soil.
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