CN101672856A - Semiautomatic biochemistry analyzer and double-wavelength testing method thereof - Google Patents
Semiautomatic biochemistry analyzer and double-wavelength testing method thereof Download PDFInfo
- Publication number
- CN101672856A CN101672856A CN 200810216085 CN200810216085A CN101672856A CN 101672856 A CN101672856 A CN 101672856A CN 200810216085 CN200810216085 CN 200810216085 CN 200810216085 A CN200810216085 A CN 200810216085A CN 101672856 A CN101672856 A CN 101672856A
- Authority
- CN
- China
- Prior art keywords
- monochromator
- wavelength
- light
- optical filter
- cuvette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a semiautomatic biochemistry analyzer which comprises a light source, a monochromator, a cuvette, a photoelectric acquisition device, a monochromator driving device and a control device, wherein the monochromator is provided with at least two optical filters with different wavelengths, the monochromator driving device is used for driving the monochromator to change positions in the testing process and ensures that at least two optical filters with corresponding tested wavelengths are arranged in an optical path in sequence so that light beams pass and are converted intomonochromatic lights; and the control device is used for controlling the photoelectric acquisition device to carry out signal acquisition to at least two monochromatic lights with different wavelengths in each acquisition period and controlling the monochromator driving device to actuate. The invention is good for improving the accuracy and the reliability of the testing result.
Description
[technical field]
The present invention relates to a kind of semi-automatic biochemical analyzer.
[background technology]
Biochemical Analyzer generally includes light source, than chrominance channel and photoelectricity harvester, semi-automatic biochemical analyzer has single beam, digital ratio chrominance channel and monochromatic light electricity harvester.Double-wavelength testing method is to grow up on traditional spectrophotometric method basis, compare with single wavelength measurement method, the influence of light source fluctuation can be eliminated or effectively be suppressed to double-wavelength testing method, effectively interfering material has a better role to the inaccurate tool of test result that may cause because of solution muddiness or the unequal defective of cuvette optical characteristics simultaneously to the influence of test result in the inhibitory reaction.In the practical application, photometer for many sense channels generally is an absorbance of measuring the primary and secondary wavelength simultaneously, time wavelength correspondence of absorbance can only timesharing measure predominant wavelength and to(for) the photometer of single sense channel, two kinds of application all are with the absorbance difference of the primary and secondary wavelength absorbance as measured matter, calculate the concentration of detected solution at last according to LB (lambert-Bill) principle.The existing double-wavelength testing method of semi-automatic biochemical analyzer that is applicable to is: the absorbance of first continuous acquisition predominant wavelength correspondence, treat that all data acquisitions of predominant wavelength carry out the collection of time all data of wavelength after intact again.The defective of this double-wavelength testing method is: 1) test period of two kinds of wavelength does not possess simultaneity, the test duration point differs bigger, even for end-point method, can not deduct the influence of light source fluctuation itself fully, in data processing, may cause other errors to test result.2) because of test duration of two kinds of wavelength point differs bigger, so existing double-wavelength testing method is not suitable for the semi-automatic biochemical analyzer that through-rate method and fixed time are analyzed.
[summary of the invention]
Fundamental purpose of the present invention solves the problems of the prior art exactly, and a kind of semi-automatic biochemical analyzer and dual wavelength analytical approach thereof are provided.
For achieving the above object, the invention provides a kind of semi-automatic biochemical analyzer, comprising: light source; Be used for by the light beam that sends by light source and be translated into monochromatic monochromator; Be used to hold the cuvette of tested sample solution, in the light path of described cuvette between described light source and described monochromator or described cuvette be arranged in the monochromatic light path that transmits from monochromator and see through the light that sends by light source; Be used for the monochromatic light that sees through described cuvette and monochromator is carried out the photoelectricity harvester of signals collecting; The monochromator drive unit, described monochromator has the optical filter of at least two different wave lengths, described monochromator drive unit is used for driving monochromator at test process and shifts one's position, make the optical filter corresponding place light path successively, and be converted into monochromatic light so that light beam passes through with at least two tested wavelength; And control device, be used to control photoelectricity harvester monochromatic light at least two different wave lengths in each collection period and carry out signals collecting successively, and control the action of described monochromator drive unit.
Described control device is used for when the optical filter corresponding with a tested wavelength is still in light path, control photoelectricity harvester begins described wavelength light is carried out signals collecting, and the described monochromator drive unit driving of control monochromator is shifted one's position after signals collecting is finished, so that the optical filter corresponding with another tested wavelength is still in the light path.
The present invention also provides a kind of double-wavelength testing method simultaneously, be applicable to semi-automatic biochemical analyzer, described semi-automatic biochemical analyzer comprises light source, monochromator, cuvette and photoelectricity harvester, described monochromator has the optical filter of at least two different wave lengths, light transmission monochromator and cuvette that described light source sends said method comprising the steps of:
A1, in test process the position of the described monochromator of conversion, make the optical filter corresponding place light path successively, and be converted into monochromatic light so that light beam passes through with at least two tested wavelength;
B1, control photoelectricity harvester carry out signals collecting successively to the monochromatic light of at least two different wave lengths by cuvette and monochromator in each collection period;
C1, circulation step A1, B1 are until the collection period number of finishing setting.Above-mentioned steps A1, B1 constitute a collection period, and according to the collection period number that the operator sets, constantly circulation step A1, B1 finish until whole test process.
Described tested wavelength is two.
Each collection period may further comprise the steps: in steps A 1, when the optical filter corresponding with first wavelength is still in the light path, control photoelectricity harvester begins described first wavelength light is carried out signals collecting, after collection is finished, the control monochromator is shifted one's position, when the optical filter corresponding with second wavelength is still in the light path, control photoelectricity harvester begins described second wavelength light is carried out signals collecting, after collection is finished, the control monochromator is shifted one's position, so that the optical filter corresponding with first wavelength is still in the light path, begin next collection period.
The invention has the beneficial effects as follows: the method for designing of test at interval, dwindle the time interval of gathering between two wavelength, more approach the actual process of carrying out of reacting, help improving precision of test result and reliability.And dual wavelength test mode of the present invention satisfies the test of rate method, fixed time and the end-point method project of semi-automatic biochemical analyzer simultaneously.
[description of drawings]
Fig. 1 is a semi-automatic biochemical analyzer list wavelength measurement schematic diagram;
Fig. 2 is the dual wavelength test philosophy figure of an embodiment of the present invention;
Fig. 3 is the dual wavelength test sequence schematic diagram of an embodiment of the present invention;
Fig. 4 is the dual wavelength test sequence schematic diagram of the another kind of embodiment of the present invention;
Fig. 5 is the multi-wavelength test sequence schematic diagram of another embodiment of the present invention.
[embodiment]
The application's feature and advantage will be elaborated in conjunction with the accompanying drawings by embodiment.
The common structure of semi-automatic biochemical analyzer as shown in Figure 1, wherein, light source 1 is the energy source of colorimetric system, can send complex light; Lens combination 2 is collected, is transmitted the complex light from light source 1, in some cases lens combination 2 can be set; Monochromator 3 will filter through collimation or the combining light of assembling, only by testing the monochromatic light of required wavelength.By behind the ratio chrominance channel of cuvette 4, unabsorbed energy carries out signals collecting by photoelectricity harvester 5, passes through follow-up conversion of signals, processing and calculating again, can obtain the concentration of tested sample through the light after filtering.
In an embodiment of the present invention, the structure of semi-automatic biochemical analyzer comprises light source 1, lens combination 2, monochromator 3, cuvette 4, photoelectricity harvester 5, monochromator drive unit 6 and control device 7 as shown in Figure 2.Light source 1 sends single beam, can not increase other light-dividing devices in the system, as optical fiber etc.Be similarly single beam by cuvette 4 than the light of chrominance channel, in the test process, the light source 1 of system, lens combination 2, cuvette 4 and photoelectricity harvester etc. all keep static, and monochromator 3 rotates according to the system design sequential.For the dual wavelength test, monochromator 3 has the optical filter of at least two different wave lengths, and selection two wavelength wherein suppose that as the test wavelength wavelength is predominant wavelength λ 1, and another wavelength is a time wavelength X 2.The time that system sequence is designed to all data of dual wavelength to be gathered requires to be divided into several same time phase according to the operator, and each time phase is a collection period.In each collection period, control device 7 (for example FPGA, microprocessor or other logic control device) control monochromator drive unit 6 instructs according to system sequence, the driving monochromator is shifted one's position, make two pairing optical filters of wavelength be arranged in light path successively, simultaneously, control device 7 control photoelectricity harvesters 5 carry out signals collecting to two kinds of monochromatic light that see through cuvette and optical filter according to system directive successively in each collection period.Repeat above-mentioned control again after a collection period finishes, begin another collection period, the collection period set up to the operator counts up to.In each collection period, be preferably equal to the acquisition time of two wavelength light.
The method that monochromator is shifted one's position can be that rotation or straight line move, and when making its evolution by rotating monochromator, monochromator is preferably disc type.When moving monochromator by straight line and make its evolution, monochromator is preferably rectangle.Drive unit 6 can be for including the drive unit of motor.
Cuvette 4 also can be at light source 1 in the light path between the monochromator 3, and the complex light that light source 1 sends is converted into monochromatic light by passing through monochromator 3 behind the ratio chrominance channel of cuvette 4 again.
The dual wavelength test sequence is shown in Fig. 3,4.Before the test, the monochromator drive unit remains a constant speed and rotates or stationary state.At t1 constantly, the operator carries out the dual wavelength test, operating system response back sends instruction to control device, send gating pulse behind the control device response instruction, control monochromator drive unit drives monochromator to begin constantly to rotate to an angle with setting speed from t2 and makes the optical filter corresponding with predominant wavelength λ 1 rotate to (this state is also referred to as the predominant wavelength position) on the light path, and light beam is passed through.Or the monochromator drive unit is carried out earlier and is driven monochromator again behind homing action and rotate to the predominant wavelength position.After monochromator rotated to the predominant wavelength position, stable within a certain period of time back kept stationary state constantly in t3.At this moment, two kinds of working methods are arranged:
1) as shown in Figure 3, after t3 moment monochromator drive unit was stable, control device promptly sent instruction predominant wavelength is carried out the photoelectricity collection, and finished the data acquisition of predominant wavelength λ 1 constantly at t4.Then, control device sends gating pulse, the monochromator drive unit with setting speed by the direct motion of predominant wavelength position to time wavelength X 2 positions (even the optical filter corresponding with inferior wavelength X 2 rotates on the light path), and carry out time wavelength data collection in the constantly static stable back of t5.In each collection period, the times of collection of every kind of wavelength can be determined that for example being set in the interior acquisition time to each wavelength of each collection period is 1 second greater than 1 time by system design, frequency acquisition is 20 times/second.T6 gathers inferior wavelength X 2 constantly and finishes.Then, system switches to the predominant wavelength position again, and the mode of switching by the primary and secondary wavelength interval successively, and above-mentioned steps is carried out in circulation, finishes the dual wavelength test in the test period that the operator sets.A test loop cycle of this sequential correspondence is t6-t2.
2) as shown in Figure 4, after t4 finishes the data acquisition of predominant wavelength λ 1 constantly, control device is not directly controlled monochromator drive unit driving monochromator and is rotated to time wavelength position, but waits for that certain hour, is moved to inferior wavelength position by the predominant wavelength position after the moment to t5 again.Wherein (t5-t2)=(t8-t5).The data acquisition of primary and secondary wavelength is carried out in circulation successively then, just carries out the wavelength switching all must satisfy time of default after finishing data acquisition after at every turn.
Predominant wavelength and time wavelength can be set according to operator's requirement in the present embodiment.
Biochemical analysis method commonly used mainly contains end-point method, rate method (claiming KINETIC METHOD again) and fixed time.Wherein, after end-point method referred to that reaction reaches balance, absorbance was directly proportional with the concentration of measured object; Rate method refers in course of reaction, reaction velocity is directly proportional with the zero degree side of concentration of substrate, reactant at the uniform velocity generates certain product, causes determined solution absorbance under a certain wavelength to reduce equably or increase, and reduces or the speed that increases is directly proportional with the activity or the concentration of measured object; Fixed time refers to that in certain reaction in the time, reaction velocity is directly proportional with the first power of concentration of substrate.Because substrate is in continuous consumption, so entire reaction speed constantly reducing, and increase (or reduction) speed that shows as absorbance is more and more littler.
The test sequence that the present invention is designed, for the single channel test macro, shortened the mistiming of testing between the primary and secondary wavelength to greatest extent, satisfy of the strict demand of test events such as rate method and fixed time to time synchronized, this method is equally applicable to the end-point method test, and be better than existing double-wavelength testing method, can effectively suppress fluctuation in the test process the influence of test result.Simultaneously, cooperate direct-connected type of drive of monochromator and micro-stepping control method, can realize the switching between the dual wavelength more fast and accurately, help the raising of measuring accuracy.
The two kinds of timing Design of comparing, method 1 in theory between the primary and secondary wavelength time of acquisition interval the shortest, more favourable to the test of rate method and fixed time, but Control System Design is had relatively high expectations; Though method 2 lagged behind to some extent in the wavelength last time of switching, and relatively met the actual working state of driving control system, and the total linear spacing cycle maintenance that the primary and secondary wavelength switches is constant, is convenient to realize control.In two kinds of test sequences, the time that predominant wavelength and time wavelength are finished once test respectively equates that the data acquisition number of times is also identical.
In addition, designed method of the present invention also can be extended to single channel multi-wavelength test specification, and monochromator has the optical filter of a plurality of wavelength, and it gathers timing Design as shown in Figure 5.During test, the monochromator drive unit drives monochromator and switches between each selected wavelength successively, and control photoelectricity harvester image data, all wavelengths is a complete collection period after all finishing a data acquisition, and then each wavelength that circulates continues to test the test period of setting to the operator.
The present invention carries out the signals collecting when being still in the light path at optical filter, can also carry out signals collecting in the process of optical filter motion.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (9)
1. semi-automatic biochemical analyzer comprises:
Light source;
Be used for by the light beam that sends by light source and be translated into monochromatic monochromator;
Be used to hold the cuvette of tested sample solution, in the light path of described cuvette between described light source and described monochromator or described cuvette be arranged in the monochromatic light path that transmits from monochromator and see through the light that sends by light source;
Be used for the monochromatic light that sees through described cuvette and monochromator is carried out the photoelectricity harvester of signals collecting; It is characterized in that also comprising:
The monochromator drive unit, described monochromator has the optical filter of at least two different wave lengths, described monochromator drive unit is used for driving monochromator at test process and shifts one's position, make the optical filter corresponding place light path successively, and be converted into monochromatic light so that light beam passes through with at least two tested wavelength; With
Control device is used to control photoelectricity harvester monochromatic light at least two different wave lengths in each collection period and carries out signals collecting successively, and controls the action of described monochromator drive unit.
2. semi-automatic biochemical analyzer as claimed in claim 1, it is characterized in that: described control device is used for when the optical filter corresponding with a tested wavelength is still in light path, control photoelectricity harvester begins described wavelength light is carried out signals collecting, and the described monochromator drive unit driving of control monochromator is shifted one's position after signals collecting is finished, so that the optical filter corresponding with another tested wavelength is still in the light path.
3. semi-automatic biochemical analyzer as claimed in claim 2 is characterized in that: described monochromator is discoid, and described monochromator drive unit comprises motor.
4. double-wavelength testing method, be applicable to semi-automatic biochemical analyzer, described semi-automatic biochemical analyzer comprises light source, monochromator, cuvette and photoelectricity harvester, described monochromator has the optical filter of at least two different wave lengths, light transmission monochromator and cuvette that described light source sends is characterized in that said method comprising the steps of:
A1, in test process the position of the described monochromator of conversion, make the optical filter corresponding place light path successively, and be converted into monochromatic light so that light beam passes through with at least two tested wavelength;
B1, control photoelectricity harvester carry out signals collecting successively to the monochromatic light of at least two different wave lengths by cuvette and monochromator in each collection period;
C1, circulation step A1, B1 are until the collection period number of finishing setting.
5. double-wavelength testing method as claimed in claim 4 is characterized in that: described tested wavelength is two.
6. double-wavelength testing method as claimed in claim 5, it is characterized in that: each collection period may further comprise the steps: in steps A 1, when the optical filter corresponding with first wavelength is still in the light path, control photoelectricity harvester begins described first wavelength light is carried out signals collecting, after collection is finished, the control monochromator is shifted one's position, when the optical filter corresponding with second wavelength is still in the light path, control photoelectricity harvester begins described second wavelength light is carried out signals collecting, after collection is finished, the control monochromator is shifted one's position, so that the optical filter corresponding with first wavelength is still in the light path, begins next collection period.
7. double-wavelength testing method as claimed in claim 6 is characterized in that: in each collection period, identical to the acquisition time of each wavelength.
8. double-wavelength testing method as claimed in claim 6 is characterized in that: in described steps A 1, come the position of the described monochromator of conversion by monochromator drive unit rotating monochromator.
9. double-wavelength testing method as claimed in claim 6 is characterized in that: after each collection is finished, control monochromator again behind the wait certain hour and shift one's position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102160851A CN101672856B (en) | 2008-09-11 | 2008-09-11 | Semiautomatic biochemistry analyzer and double-wavelength testing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102160851A CN101672856B (en) | 2008-09-11 | 2008-09-11 | Semiautomatic biochemistry analyzer and double-wavelength testing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101672856A true CN101672856A (en) | 2010-03-17 |
| CN101672856B CN101672856B (en) | 2013-11-27 |
Family
ID=42020171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102160851A Active CN101672856B (en) | 2008-09-11 | 2008-09-11 | Semiautomatic biochemistry analyzer and double-wavelength testing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101672856B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020103051A1 (en) * | 2018-11-21 | 2020-05-28 | 北京普利生仪器有限公司 | Method for measuring sample absorbance difference, sample analyzer and storage medium |
| CN112534269A (en) * | 2018-08-22 | 2021-03-19 | 株式会社日立高新技术 | Automatic analysis device and automatic analysis system |
| CN113056230A (en) * | 2018-10-12 | 2021-06-29 | 深圳迈瑞生物医疗电子股份有限公司 | Medical device |
| WO2023041074A1 (en) * | 2021-09-17 | 2023-03-23 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer, and control method for sample analyzer |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85202765U (en) * | 1985-06-29 | 1986-04-30 | 上海自立仪器合作工厂 | Concentration direct-reading dial wivelength photometer |
| JP2003090794A (en) * | 2001-09-20 | 2003-03-28 | Furuno Electric Co Ltd | Colorimetric absorbance measuring apparatus |
| CN100549669C (en) * | 2006-09-08 | 2009-10-14 | 深圳迈瑞生物医疗电子股份有限公司 | Enzyme mark instrument spectrophotometric detecting optical system and diaphragm thereof and optical filter wheel |
-
2008
- 2008-09-11 CN CN2008102160851A patent/CN101672856B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112534269A (en) * | 2018-08-22 | 2021-03-19 | 株式会社日立高新技术 | Automatic analysis device and automatic analysis system |
| CN112534269B (en) * | 2018-08-22 | 2024-04-05 | 株式会社日立高新技术 | Automatic analysis device and automatic analysis system |
| CN113056230A (en) * | 2018-10-12 | 2021-06-29 | 深圳迈瑞生物医疗电子股份有限公司 | Medical device |
| WO2020103051A1 (en) * | 2018-11-21 | 2020-05-28 | 北京普利生仪器有限公司 | Method for measuring sample absorbance difference, sample analyzer and storage medium |
| WO2023041074A1 (en) * | 2021-09-17 | 2023-03-23 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer, and control method for sample analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101672856B (en) | 2013-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101672856A (en) | Semiautomatic biochemistry analyzer and double-wavelength testing method thereof | |
| US4818705A (en) | Method and apparatus for analyzing the composition of the exhaust gas of any internal combustion engine | |
| CN201476879U (en) | Fast spectrum analysis system with synchronous scanning and sampling | |
| CN101726359B (en) | Multiple-grating monochrometer and calibration method thereof | |
| US20100067003A1 (en) | Time-resolved and wavelength-resolved spectroscopy for characterizing biological materials | |
| EP0456813B1 (en) | Multichannel optical monitoring system | |
| CN101477050B (en) | Polycyclic aromatic hydrocarbon three-dimensional fluorescent on-line detecting instrument | |
| CN211318188U (en) | Optical detection system and sample analyzer | |
| CN110553992A (en) | Infrared spectrum high-speed measurement system and method | |
| CN204903484U (en) | Ultraviolet capillary column goes up check out test set | |
| CN119000574A (en) | Ultraviolet visible light detector for realizing three-dimensional scanning | |
| CN112987197B (en) | Optical device automatic coupling system based on FPGA and data acquisition method thereof | |
| CN205749270U (en) | A kind of drug-beam infrared spectrophotometer with double infrared light detectors | |
| CN108051086B (en) | A method for frequency conversion measurement of infrared spectrum | |
| CN215768615U (en) | Biochemical photoelectric detection system and biochemical analyzer | |
| JPS5824825A (en) | Plural wavelength photometer | |
| CN102932053A (en) | Monitor and monitoring method for optical communication channel | |
| JPS62157537A (en) | Signal processing method for spectrometer | |
| JP2008096241A (en) | Spectrophotometer | |
| CN203866320U (en) | Fluorescence detection optical path device for single-excitation double-path detection | |
| JP2014035224A (en) | Spectrophotometer | |
| JP2004226257A (en) | Multi-port spectroscope and optical spectrum analyzer using the same | |
| CN217738983U (en) | Microscopic circular dichroism spectrum detection system based on single photon counting and collecting method | |
| CN221124306U (en) | High-flux spectrum measuring device | |
| JPS6182169A (en) | Flow injection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20100317 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: A semi-automatic biochemical analyzer and its dual-wavelength testing method Granted publication date: 20131127 License type: Common License Record date: 20220804 |
|
| EE01 | Entry into force of recordation of patent licensing contract |