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CN101671455B - 应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶 - Google Patents

应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶 Download PDF

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Publication number
CN101671455B
CN101671455B CN2009100952314A CN200910095231A CN101671455B CN 101671455 B CN101671455 B CN 101671455B CN 2009100952314 A CN2009100952314 A CN 2009100952314A CN 200910095231 A CN200910095231 A CN 200910095231A CN 101671455 B CN101671455 B CN 101671455B
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eva
polyethylene
bottle
flask
cell culture
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CN101671455A (zh
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惠觅宙
原世平
赵兴年
李峦峰
胡福林
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Zhejiang JYSS Bio Engineering Co Ltd
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Hangzhou AmProtein Bioengineering Co Ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C48/00—Extrusion moulding, i.e. expressing the moulding material through a die or nozzle which imparts the desired form; Apparatus therefor
    • B29C48/25—Component parts, details or accessories; Auxiliary operations
    • B29C48/88—Thermal treatment of the stream of extruded material, e.g. cooling
    • B29C48/918—Thermal treatment of the stream of extruded material, e.g. cooling characterized by differential heating or cooling
    • B29C48/9185—Thermal treatment of the stream of extruded material, e.g. cooling characterized by differential heating or cooling in the direction of the stream of the material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C48/00—Extrusion moulding, i.e. expressing the moulding material through a die or nozzle which imparts the desired form; Apparatus therefor
    • B29C48/03—Extrusion moulding, i.e. expressing the moulding material through a die or nozzle which imparts the desired form; Apparatus therefor characterised by the shape of the extruded material at extrusion
    • B29C48/04—Particle-shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C48/00—Extrusion moulding, i.e. expressing the moulding material through a die or nozzle which imparts the desired form; Apparatus therefor
    • B29C48/25—Component parts, details or accessories; Auxiliary operations
    • B29C48/36—Means for plasticising or homogenising the moulding material or forcing it through the nozzle or die
    • B29C48/395—Means for plasticising or homogenising the moulding material or forcing it through the nozzle or die using screws surrounded by a cooperating barrel, e.g. single screw extruders
    • B29C48/40—Means for plasticising or homogenising the moulding material or forcing it through the nozzle or die using screws surrounded by a cooperating barrel, e.g. single screw extruders using two or more parallel screws or at least two parallel non-intermeshing screws, e.g. twin screw extruders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C48/00—Extrusion moulding, i.e. expressing the moulding material through a die or nozzle which imparts the desired form; Apparatus therefor
    • B29C48/25—Component parts, details or accessories; Auxiliary operations
    • B29C48/92—Measuring, controlling or regulating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C2948/00—Indexing scheme relating to extrusion moulding
    • B29C2948/92—Measuring, controlling or regulating
    • B29C2948/92504—Controlled parameter
    • B29C2948/92514—Pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C2948/00—Indexing scheme relating to extrusion moulding
    • B29C2948/92—Measuring, controlling or regulating
    • B29C2948/92504—Controlled parameter
    • B29C2948/92704—Temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C2948/00—Indexing scheme relating to extrusion moulding
    • B29C2948/92—Measuring, controlling or regulating
    • B29C2948/92819—Location or phase of control
    • B29C2948/92857—Extrusion unit
    • B29C2948/92876—Feeding, melting, plasticising or pumping zones, e.g. the melt itself
    • B29C2948/92895—Barrel or housing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C2948/00—Indexing scheme relating to extrusion moulding
    • B29C2948/92—Measuring, controlling or regulating
    • B29C2948/92819—Location or phase of control
    • B29C2948/92923—Calibration, after-treatment or cooling zone

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本发明涉及应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶，其特征在于：其是首先将原料颗粒PE与EVA按配比混合后，再通过双螺杆挤丝机重新进行造粒；最后将颗粒吹成瓶子；上述PE与EVA的配比为：PE60～80％，EVA 20～40％。本发明的优点：1、PE通过添加适量的EVA进行制作瓶子，其产品利于成型；同时，在显微镜下观察，瓶壁内部有细微的凹突不平，进一步扩大了细胞培养液面与瓶壁的接触面积，增加传氧，更利于细胞的培养。2、塑料成本低，可灭菌并可多次使用。3、同时，PE和EVA均属于可降解成分。

Description

应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶
技术领域
本发明涉及细胞培养瓶，特别涉及的是应用聚乙烯(PE)和聚乙烯-乙酸乙烯酯共聚物(EVA)制作细胞培养瓶。
背景技术
我国是塑料大国，塑料资源丰富，价钱便宜。为了满足细胞培养的需要，我们先后使用聚碳酸酯(PC)、聚丙烯(PP)、聚乙烯(PE)等材料单独制作瓶子，从中发现使用这三种材料制作的瓶子均不同程度的影响细胞的生长；而后发现使用EVA材料制作的瓶子，其比较利于细胞的生长，细胞培养的密度及表达量较高，但其材质柔软，不利于产品的成型。
发明内容
为了克服上述技术上的缺陷以及对细胞培养更为有利，本发明提供了应用PE和EVA制作细胞培养瓶的过程和方法。
为了实现上述目的，本发明采用如下技术方案：
应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶，其步骤如下：
1、将原料颗粒PE和EVA按配比混合后，通过双螺杆挤丝机进行造粒。
(1)PE与EVA的配比为：PE60～80％，EVA20～40％。
(2)造粒设备选用直径65mm双螺杆挤丝机，其工作参数为如下：
温度1段210℃、2段200℃、3段195℃、4段190℃
挤出压力85磅
(3)原料颗粒使用烘干机进行干燥后备用。
烘干参数温度65℃-70℃
时间90分钟
2、成型(管胚→吹瓶)采用2步法吹瓶进行制作
(1)管胚(使用注塑法成型)
设备300g-450g注塑机
工作参数
温度1段205℃2段200℃3段190℃
压力一级射胶55磅
二级射胶53磅
保压53磅
时间6分钟
(2)吹瓶
瓶胚通过烤箱进行预热后开始吹瓶
吹瓶设备1mL-30L单工位全自动中空机
工作参数吹气时间15秒
吹气压力25kg/m2
冷却时间110秒
优点：
1、PE通过添加适量的EVA进行制作瓶子，其产品利于成型；同时，在显微镜下观察，瓶壁内部有细微的凹突不平，进一步扩大了细胞培养液面与瓶壁的接触面积，增加传氧，更利于细胞的培养。
2、塑料成本低，可灭菌并可多次使用。
3、同时，PE和EVA均属于可降解成分。
具体实施方案
下面参照实施例进一步说明。
实施例1、应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶，其是首先将原料颗粒PE与EVA按照8∶2的比例进行混合，再通过双螺杆挤丝机进行造粒；将颗粒通过吹瓶设备吹成瓶子。
造粒设备选用直径65mm的双螺杆挤丝机，其工作参数为：
温度1段210℃、2段200℃、3段195℃、4段190℃；
挤出压力85磅；
原料颗粒用烘干机进行干燥备用
烘干参数温度65℃-70℃
时间90分钟
成型(管胚→吹瓶)采用2步法吹瓶进行加工
1、管胚采用注塑法进行成型
设备选用300g-450g注塑机，工作参数如下：
温度1段205℃2段200℃3段190℃
压力一级射胶55磅
二级射胶53磅
保压53磅
时间6分钟
2、吹瓶
瓶胚通过烤箱预热后开始吹瓶
吹瓶设备1mL-30L单工位全自动中空机，工作参数如下：
吹气时间15秒
吹气压力25kg/m2
冷却时间110秒。
实施例2、应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶，其是首先将原料颗粒PE和EVA按照7∶3的比例进行混合，再通过双螺杆挤丝机进行造粒；将颗粒通过吹瓶设备吹成瓶子。其混合后造粒方式及吹瓶方式与实施例1一样。

Claims (1)

1.应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物的细胞培养瓶，其特征在于：原料是PE与EVA，PE与EVA的配比为：PE 70％，EVA 30％；其制作方法是首先将原料颗粒PE与EVA按配比混合后，再通过双螺杆挤丝机进行造粒；再将颗粒吹成瓶子；造颗设备选用直径65mm双螺杆挤丝机，其工作参数为：温度1段210℃、2段200℃、3段195℃、4段190℃；挤出压力85磅；原料用烘干机进行干燥备用，其烘干参数为：
温度65℃-70℃，时间为60～90分钟；
成型管胚→吹瓶采用2步法吹瓶成型：
(1)管胚使用注塑法进行成型：
设备：300g-450g注塑机
工作参数：
Figure FSB00000824692700011
(2)吹瓶
瓶胚通过烤箱进行预热后开始吹瓶，其设备选用1mL-30L单x位全自动中空机，工作参数为：吹气时间15秒，吹气压力25kg/m2，冷却时间110秒。
CN2009100952314A 2009-01-05 2009-01-05 应用聚乙烯和聚乙烯-乙酸乙烯酯共聚物制作细胞培养瓶 Active CN101671455B (zh)

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Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
CN102189732A (zh) * 2011-03-07 2011-09-21 苏州海顺包装材料有限公司 一种多层共挤细胞培养容器袋包装膜

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN1139378A (zh) * 1994-11-22 1997-01-01 巴克斯特国际有限公司 血液成分的贮存容器
US20050032205A1 (en) * 2003-08-05 2005-02-10 Smith Sidney T. In vitro cell culture employing a fibrin network in a flexible gas permeable container
WO2008135991A2 (en) * 2007-05-07 2008-11-13 Protalix Ltd. Large scale disposable bioreactor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1139378A (zh) * 1994-11-22 1997-01-01 巴克斯特国际有限公司 血液成分的贮存容器
US20050032205A1 (en) * 2003-08-05 2005-02-10 Smith Sidney T. In vitro cell culture employing a fibrin network in a flexible gas permeable container
WO2008135991A2 (en) * 2007-05-07 2008-11-13 Protalix Ltd. Large scale disposable bioreactor

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