CN101664167A - Peanut water-soluble dietary fiber enzymatic extracting method - Google Patents
Peanut water-soluble dietary fiber enzymatic extracting method Download PDFInfo
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Abstract
The invention discloses a peanut water-soluble dietary fiber enzymatic extracting method which comprises the steps of: pretreating the prepared peanut powder by acetone, alcohol, protease and alpha-amylase, and hydrolyzing by using cellulose for three times; and concentrating hydrolysis liquid and then precipitating by absolute ethyl alcohol, centrifuging and drying to obtain the water-soluble dietary fiber. The product prepared by the invention contains 30-60% of non-starch polysaccharide, can reduce the content of low density lipoprotein (LDL) cholesterol in the blood, promotes the growth and the propagation of probiotics group in the intestinal tract, and can be taken as functional food for preventing and alleviating symptom as well as treating diseases such as cardiovascular diseases and the like in an auxiliary way. The method is low in production cost, high in fiber content and suitable for industrialized production.
Description
Technical field
The present invention relates to a kind of peanut water-soluble dietary fiber enzymatic extracting method, belong to food processing field.
Background technology
Dietary fiber is meant in the food summation of plant cell, polysaccharide, lignin and other material that can not be digested and assimilated by the human body endogenous enzymes, and this definition comprises in the food a large amount of constituents such as cellulose, hemicellulose, lignin, colloid, modified cellulose, cement, oligosaccharides, pectin and other constituent such as wax, cutin, cork matter in a small amount.Though dietary fiber is not had a nutritive value, can treat and prevent many modern civilization diseases, the eubolism of human body is absolutely necessary.Therefore, dietary fiber has been subjected to the expert from different field, comprise physician, nutritionist, meals man, Food Science man, biochemist, and the relevant science decision person's of alimentary codex and nutrition education extensive attention, and classify it as after carbohydrate, protein, fat, water, minerals and vitamins " the seventh-largest nutrient ".Western developed country just sets about dietary fiber is carried out research and development as far back as twentieth century the seventies.And China's tradition meals can not lack dietary fiber based on plant food, thereby research and development utilizes not enough to dietary fiber.We can say that China still is in the starting stage to the research and development of dietary fiber at present, does not form the processing enterprise of scale.But along with improving constantly of people's living standard, great changes have taken place in eating habit, lack cellulosic situation in the meals and are on the rise, and rich man's diseases such as the disease of causeing fat, hypertension, coronary heart disease, diabetes, colon cancer are more and more general.Therefore, actively develop dietary fiber research and utilization and have extremely great realistic significance improving China people general level of the health.China's peanut annual production is big, and the byproduct of peanut industry is potential dietary fiber resource.The peanut dietary fiber has nutrition, health care and physiologic effect, can add in the numerous food, to improve nutrition, the health-care efficacy of food.Research and development peanut dietary fiber is extremely important to health, also has huge social benefit and economic benefit simultaneously.
In order to improve and to improve national diet structure, improve national physique holistic health level, and utilization rationally has the natural agriculture resource now to large extent, improve the food resource benefit, will develop dietary fiber food, and preparation technology provided by the present invention can process the peanut water-soluble dietary fiber product, can satisfy above-mentioned needs.
Summary of the invention
The purpose of this invention is to provide the extracting method that a kind of cost is low, the product dietary fiber content is high.
For achieving the above object, the present invention adopts following steps:
With the peanut powder that makes after acetone, ethanol, protease and AMS preliminary treatment, again through three hydrolysis of cellulase, hydrolyzate through concentrate, absolute ethyl alcohol precipitation, centrifugal, drying obtain water-soluble dietary fiber.
Specifically, described method is as follows:
(1) preparation of peanut powder;
(2) gained peanut powder is added acetone, water bath with thermostatic control oscillation treatment certain hour, suction filtration is abandoned filtrate, keeps filter residue 1, and filter residue 1 adds ethanol, water bath with thermostatic control oscillation treatment certain hour, suction filtration is abandoned filtrate, keeps filter residue 2;
(3) mixed enzyme solution of adding protease and AMS in the filter residue 2, water bath with thermostatic control oscillation treatment certain hour, suction filtration keeps filtrate 1, obtains filter residue 3.The washing of filter residue 3 usefulness distilled water, suction filtration keeps filtrate 2, obtains filter residue 4;
(4) filter residue 4 is divided into 2.5 parts, get a filter residue 4 and add cellulase solution, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration, keep filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again with cellulase solution, water bath with thermostatic control oscillation treatment certain hour, suction filtration keeps filtrate 4, obtains filter residue 6;
(5) add moiety filter residue 4 in the filter residue 6, add the above-mentioned cellulase solution of a half strength again, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration keeps filtrate 5;
(6) filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2;
(7) be dissolved in the boiling water after precipitation 1 and precipitation 2 merge, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitate 3 dryings, pulverize and to obtain the peanut water-soluble dietary fiber product.
Preferably, the described peanut powder, preparation method thereof of step (1) is that raw material is cleaned up with flowing water, 80 ℃ of dryings in air dry oven, and dried raw material is pulverized with the plant pulverizer, crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber;
Described raw material does not go rotten for having, peanut shell, peanut bar and the peanut root of free from insect pests; Described peanut powder is the powder behind peanut shell, peanut bar and the peanut root crushing screening.
Preferably, the described acetone addition of step (2) is 3～5 times (peanut opaque amount and acetone volume ratios) of peanut powder, and the temperature of handling the peanut powder is 30～50 ℃, and the processing time is 30～90min;
Preferably, described amount of alcohol added is 3～5 times (peanut opaque amount and ethanol volume ratios) of peanut powder.
Preferably, the described protease of step (3) is any or several in papain, neutral proteinase and the alkali protease;
Preferably, the quality percentage composition of protease is 0.2%～1.0% in the described mixed enzyme solution, and the quality percentage composition of AMS is 0.2%～1.0%, the pH value 6.0～8.0 of mixed enzyme solution, the mixed enzyme solution processing time is 1.0～3.0h, and treatment temperature is 40～60 ℃.
Preferably, the concentration of the described cellulase solution of step (4) is 0.05～2.0mg/ml, and the pH value is 4.0～6.0, and treatment temperature is 35～60 ℃, and the processing time is 0.5～8.0h.
Preferably, the water bath with thermostatic control oscillation treatment certain hour described in step (2), (3) and (4) is meant that the temperature of handling the peanut powder is 40～60 ℃, and the processing time is 20～60min.
Preferably, the drying means of described precipitation 3 is any in freeze drying and the vacuum drying.
Suction filtration described in the said method is to adopt suction filtration method well known in the art to operate.
The water-soluble dietary fiber product is a kind of khaki powder, wherein contains non-starchiness polysaccharide 30%～60%, swelling in 100 ℃ of water of pH6.0～7.0, and the dissolving back is a brown yellow solution, has a small amount of insoluble matter to exist.The water-soluble dietary fiber product can reduce low-density lipoprotein in the blood (LDL) content of cholesterol, promotes beneficial flora growth and breeding in the enteron aisle, can be used as the functional food of diseases such as prevention, mitigation symptoms, adjuvant therapy of cardiovascular.Method production cost of the present invention is low, fiber content is high owing to adopt, and is fit to suitability for industrialized production.
The specific embodiment
Present embodiment is to be used for understanding, rather than restriction the present invention.The inventor is tested confirmation, and step that method of the present invention adopted and parameter all can realize purpose of the present invention.
Embodiment 1
Get do not have to go rotten, peanut shell, peanut bar and the peanut root of free from insect pests clean up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 3 times of (peanut opaque amount and acetone volume ratio) acetone, 30 ℃ of water bath with thermostatic control oscillation treatment 30min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 3 times of (peanut opaque amount and ethanol volume ratio) ethanol, 30 ℃ of water bath with thermostatic control oscillation treatment 30min, suction filtration is abandoned filtrate, keeps filter residue 2; The mixed enzyme solution that adds papain and AMS in the filter residue 2, the quality percentage composition of protease is 0.2% in the mixed enzyme solution, and the quality percentage composition of AMS is 0.2%, the pH value 6.0 of mixed enzyme solution, 40 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 1.0h, suction filtration keeps filtrate 1, obtains filter residue 3, filter residue 3 usefulness distilled water wash, suction filtration keeps filtrate 2, obtains filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 0.05mg/ml, the pH value is 4.0 cellulase solution,, 35 ℃ of water bath with thermostatic control oscillation treatment 0.5h, suction filtration, keep filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, the cushioning liquid that adds identical pH value again with cellulase solution, 35 ℃ of water bath with thermostatic control oscillation treatment 0.5h, suction filtration keeps filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, add the cellulase solution of a half strength again, temperature is 35 ℃ of water bath with thermostatic control oscillation treatment 0.5h, suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitation 3 obtains the peanut water-soluble dietary fiber product through freeze drying, pulverizing.
Embodiment 2
Get do not have to go rotten, peanut shell, peanut bar and the peanut root of free from insect pests clean up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 5 times of (peanut opaque amount and acetone volume ratio) acetone, 50 ℃ of water bath with thermostatic control oscillation treatment 90min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 5 times of (peanut opaque amount and ethanol volume ratio) ethanol, 50 ℃ of water bath with thermostatic control oscillation treatment 90min, suction filtration is abandoned filtrate, keeps filter residue 2; The mixed enzyme solution that adds alkali protease and AMS in the filter residue 2, the quality percentage composition of protease is 1.0% in the mixed enzyme solution, and the quality percentage composition of AMS is 1.0%, the pH value 8.0 of mixed enzyme solution, 60 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 3.0h, suction filtration keeps filtrate 1, obtains filter residue 3, filter residue 3 usefulness distilled water wash, suction filtration keeps filtrate 2, obtains filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 2.0mg/ml, the pH value is 6.0 cellulase solution, 60 ℃ of water bath with thermostatic control oscillation treatment 8.0h, suction filtration keeps filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again, 60 ℃ of water bath with thermostatic control oscillation treatment 8.0h with cellulase solution, suction filtration, keep filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, the cellulase solution that adds a half strength again, temperature is 60 ℃ of water bath with thermostatic control oscillation treatment 8.0h, and suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitation 3 obtains the peanut water-soluble dietary fiber product through vacuum drying, pulverizing.
Embodiment 3
Get do not have to go rotten, peanut shell, peanut bar and the peanut root of free from insect pests clean up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 4 times of (peanut opaque amount and acetone volume ratio) acetone, 40 ℃ of water bath with thermostatic control oscillation treatment 60min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 4 times of (peanut opaque amount and ethanol volume ratio) ethanol, 40 ℃ of water bath with thermostatic control oscillation treatment 60min, suction filtration is abandoned filtrate, keeps filter residue 2; The mixed enzyme solution that adds protease neutral proteinase and AMS in the filter residue 2, the quality percentage composition of protease is 0.6% in the mixed enzyme solution, and the quality percentage composition of AMS is 0.6%, the pH value 7.0 of mixed enzyme solution, 50 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 2.0h, suction filtration keeps filtrate 1, obtains filter residue 3, filter residue 3 usefulness distilled water wash, suction filtration keeps filtrate 2, obtains filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 0.95mg/ml, the pH value is 5.0 cellulase solution,, 48 ℃ of water bath with thermostatic control oscillation treatment 4.0h, suction filtration, keep filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, the cushioning liquid that adds identical pH value again with cellulase solution, 48 ℃ of water bath with thermostatic control oscillation treatment 4.0h, suction filtration keeps filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, add the cellulase solution of a half strength again, temperature is 48 ℃ of water bath with thermostatic control oscillation treatment 4.0h, suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitate 3 drying freeze dryings, pulverize and to obtain the peanut water-soluble dietary fiber product.
Embodiment 4
Get do not have to go rotten, peanut shell, peanut bar and the peanut root of free from insect pests clean up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 3.5 times of (peanut opaque amount and acetone volume ratio) acetone, 35 ℃ of water bath with thermostatic control oscillation treatment 45min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 3.5 times of (peanut opaque amount and ethanol volume ratio) ethanol, 35 ℃ of water bath with thermostatic control oscillation treatment 45min, suction filtration is abandoned filtrate, keeps filter residue 2; The mixed enzyme solution that adds papain, neutral proteinase and AMS in the filter residue 2, the quality percentage composition of protease is 0.4% in the mixed enzyme solution, and the quality percentage composition of AMS is 0.4%, the pH value 6.5 of mixed enzyme solution, 45 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 1.5h, suction filtration keeps filtrate 1, obtains filter residue 3, filter residue 3 usefulness distilled water wash, suction filtration keeps filtrate 2, obtains filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 0.6mg/ml, the pH value is 4.5 cellulase solution, 40 ℃ of water bath with thermostatic control oscillation treatment 2.0h, suction filtration keeps filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again, 40 ℃ of water bath with thermostatic control oscillation treatment 2.0h with cellulase solution, suction filtration, keep filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, the cellulase solution that adds a half strength again, temperature is 40 ℃ of water bath with thermostatic control oscillation treatment 2.0h, and suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitation 3 obtains the peanut water-soluble dietary fiber product through vacuum drying, pulverizing.
Embodiment 5
Getting raw material (not having peanut shell, peanut bar and peanut root mildew and rot, free from insect pests) cleans up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 4.5 times of (peanut opaque amount and acetone volume ratio) acetone, 45 ℃ of water bath with thermostatic control oscillation treatment 75min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 4.5 times of (peanut opaque amount and ethanol volume ratio) ethanol, 45 ℃ of water bath with thermostatic control oscillation treatment 75min, suction filtration is abandoned filtrate, keeps filter residue 2; The mixed enzyme solution that adds protease neutral proteinase, alkali protease and AMS in the filter residue 2, the quality percentage composition of protease is 0.8% in the mixed enzyme solution, and the quality percentage composition of AMS is 0.8%, the pH value 7.5 of mixed enzyme solution, 55 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 2.5h, suction filtration keeps filtrate 1, obtains filter residue 3, filter residue 3 usefulness distilled water wash, suction filtration keeps filtrate 2, obtains filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 1.5mg/ml, the pH value is 5.5 cellulase solution, 55 ℃ of water bath with thermostatic control oscillation treatment 6.0h, suction filtration keeps filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again, 55 ℃ of water bath with thermostatic control oscillation treatment 6.0h with cellulase solution, suction filtration, keep filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, the cellulase solution that adds a half strength again, temperature is 55 ℃ of water bath with thermostatic control oscillation treatment 6.0h, and suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitation 3 obtains the peanut water-soluble dietary fiber product through vacuum drying, pulverizing.
Embodiment 6
Getting raw material (not having peanut shell, peanut bar and peanut root mildew and rot, free from insect pests) cleans up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, and crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber; The peanut powder adds 3 times of (peanut opaque amount and acetone volume ratio) acetone, 50 ℃ of water bath with thermostatic control oscillation treatment 90min, suction filtration, abandon filtrate, keep filter residue 1, filter residue 1 adds 5 times of (peanut opaque amount and ethanol volume ratio) ethanol, 30 ℃ of water bath with thermostatic control oscillation treatment 30min, suction filtration is abandoned filtrate, keeps filter residue 2; Add protease papain, neutral proteinase and alkali protease in the filter residue 2, and the mixed enzyme solution of AMS, the quality percentage composition of protease is 0.9% in the mixed enzyme solution, the quality percentage composition of AMS is 0.3%, the pH value 6.2 of mixed enzyme solution, 42 ℃ of water bath with thermostatic control oscillation treatment of mixed enzyme solution 1.8h, suction filtration, keep filtrate 1, obtain filter residue 3, the washing of filter residue 3 usefulness distilled water, suction filtration, keep filtrate 2, obtain filter residue 4; Filter residue 4 is divided into 2.5 parts, getting a filter residue 4 adding concentration is 1.8mg/ml, the pH value is 4.5 cellulase solution, 58 ℃ of water bath with thermostatic control oscillation treatment 1.0h, suction filtration keeps filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again, 37 ℃ of water bath with thermostatic control oscillation treatment 7.0h with cellulase solution, suction filtration, keep filtrate 4, obtain filter residue 6, add moiety filter residue 4 in the filter residue 6, the cellulase solution that adds a half strength again, temperature is 50 ℃ of water bath with thermostatic control oscillation treatment 3.0h, and suction filtration keeps filtrate 5; Filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of volume absolute ethyl alcohols of concentrate 1 in the concentrate 1, standing over night, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of volume absolute ethyl alcohols of concentrate 2 in the concentrate 2, standing over night, centrifugal, keep precipitation 2; After merging, precipitation 1 and precipitation 2 be dissolved in the boiling water, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of volume absolute ethyl alcohols of concentrate 3 in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitation 3 obtains the peanut water-soluble dietary fiber product through vacuum drying, pulverizing.
Claims (10)
1, a kind of peanut water-soluble dietary fiber enzymatic extracting method may further comprise the steps:
With the peanut powder that makes after acetone, ethanol, protease and AMS preliminary treatment, again through three hydrolysis of cellulase, hydrolyzate through concentrate, absolute ethyl alcohol precipitation, centrifugal, drying obtain water-soluble dietary fiber.
2, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1 is characterized in that, may further comprise the steps:
(1) preparation of peanut powder;
(2) gained peanut powder is added acetone, water bath with thermostatic control oscillation treatment certain hour, suction filtration is abandoned filtrate, keeps filter residue 1, and filter residue 1 adds ethanol, water bath with thermostatic control oscillation treatment certain hour, suction filtration is abandoned filtrate, keeps filter residue 2;
(3) mixed enzyme solution of adding protease and AMS in the filter residue 2, water bath with thermostatic control oscillation treatment certain hour, suction filtration keeps filtrate 1, obtains filter residue 3, the washing of filter residue 3 usefulness distilled water, suction filtration keeps filtrate 2, obtains filter residue 4;
(4) filter residue 4 is divided into 2.5 parts, get a filter residue 4 and add cellulase solution, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration, keep filtrate 3, obtain filter residue 5, add a filter residue 4 in the filter residue 5, add the cushioning liquid of identical pH value again with cellulase solution, water bath with thermostatic control oscillation treatment certain hour, suction filtration keeps filtrate 4, obtains filter residue 6;
(5) add moiety filter residue 4 in the filter residue 6, add the above-mentioned cellulase solution of a half strength again, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration keeps filtrate 5;
(6) filtrate 1-filtrate 5 is merged together, and behind the enzyme 10min that goes out in boiling water bath, rotary evaporation in vacuo concentrates, obtain concentrate 1, add 4 times of absolute ethyl alcohols, standing over night in the concentrate 1, centrifugal, keep precipitation 1, obtain supernatant 1, supernatant 1 concentrates through rotary evaporation in vacuo, obtain concentrate 2, add 4 times of absolute ethyl alcohols, standing over night in the concentrate 2, centrifugal, keep precipitation 2;
(7) be dissolved in the boiling water after precipitation 1 and precipitation 2 merge, centrifugal, obtain supernatant 2, supernatant 2 concentrates through rotary evaporation in vacuo, obtains concentrate 3, adds 4 times of absolute ethyl alcohols in the concentrate 3, standing over night, centrifugal, obtain precipitating 3, precipitate 3 dryings, pulverize and to obtain the peanut water-soluble dietary fiber product.
3, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1, it is characterized in that, the described peanut powder, preparation method thereof of step (1) is that raw material is cleaned up with flowing water, 80 ℃ of dryings in air dry oven, dried raw material is pulverized with the plant pulverizer, crushed material is crossed 50 mesh sieves, collects screenings as the peanut powder that extracts water-soluble dietary fiber.
4, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1 is characterized in that, the described acetone addition of step (2) is long-pending 3～5 times of peanut powder, and the temperature of handling the peanut powder is 30～50 ℃, and the processing time is 30～90min.
5, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1 is characterized in that, the described amount of alcohol added of step (2) is long-pending 3～5 times of peanut powder.
6, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1 is characterized in that, the described protease of step (3) is any or several in papain, neutral proteinase and the alkali protease.
7, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1, it is characterized in that, the quality percentage composition of protease is 0.2%～1.0% in the described mixed enzyme solution, the quality percentage composition of AMS is 0.2%～1.0%, the pH value 6.0～8.0 of mixed enzyme solution, the mixed enzyme solution processing time is 1.0～3.0h, and treatment temperature is 40～60 ℃.
8, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1, it is characterized in that the concentration of the described cellulase solution of step (4) is 0.05～2.0mg/ml, the pH value is 4.0～6.0, treatment temperature is 35～60 ℃, and the processing time is 0.5～8.0h.
9, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1, it is characterized in that, water bath with thermostatic control oscillation treatment certain hour described in step (2), (3) and (4) is meant that the temperature of handling the peanut powder is 40～60 ℃, and the processing time is 20～60min.
10, peanut water-soluble dietary fiber enzymatic extracting method according to claim 1 is characterized in that, the drying means of described precipitation 3 is freeze drying or vacuum drying.
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