CN101650276A - System of detecting concentration of sugar in fermentation process on line - Google Patents

Abstract

The invention relates to an on-line sugar concentration detection system in the fermentation process. The input of a sugar concentration analyzer is connected to a mixing container; the inlet of the mixing container is connected to the outlets of two constant-flow pumps through a pipeline, and the first constant-flow pump is connected to a fermentation tank. Sampling in the fermentation tank; the second constant flow pump continuously takes pure water, and the liquid of the two constant flow pumps flows into the mixing container and is mixed and diluted, and then sent to the sugar concentration analyzer for online detection; the sugar concentration analyzer, the first Both the first constant-flow pump and the second constant-flow pump are connected to the controller, and the controller collects the data of the sugar concentration analyzer in real time, and sets the dilution factor according to the detection concentration range of the sugar concentration analyzer to control the operation of the two constant-flow pumps . The advantages of the present invention are: the user can set various parameters of the constant flow and sugar analyzer through the computer, control its operating status, collect sugar concentration online, and realize functions such as online sugar concentration display, statistical analysis, trend chart, and print report.

Description

On-line sugar concentration detection system during fermentation

technical field

The invention relates to an on-line detection system, in particular to an on-line sugar concentration detection system in a fermentation process.

Background technique

During the fermentation process, sugar is used as a nutrient for the growth of bacteria. If the concentration is too high or too low, it will affect the yield of bacteria and products. Therefore, it is very important to realize the monitoring of sugar concentration in the fermenter to increase the yield. However, the detection range of existing online sugar concentration detectors in the industry is limited, and the development, use and maintenance are inconvenient, and secondary development is often required to be integrated into the existing fermentation process control system.

In order to improve product quality, there are generally two commonly used sugar concentration detection methods, one is an offline analysis method, and the other is a logic estimation method. In the first method, due to the influence of artificial sampling and other factors, the acquisition of information has a large lag (the lag time is generally more than 20 minutes), and there is a large deviation between the detected concentration and the current actual concentration in the fermenter. , resulting in a large waste of monitoring or operating costs, suitable for some extensive production. Another method is to establish offline variables that can be detected online based on the interaction relationship between the external growth environment parameters of the bacteria, such as temperature, dissolved oxygen, pH, etc. and the logic estimation model of sugar concentration. The detected variables are substituted into the logical estimation model to estimate the sugar concentration in real time. This method has good real-time performance, but it is very difficult to establish an accurate logical estimation model.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies in the prior art and provide an online sugar concentration detection system in the fermentation process. The system controls the flow rate of two constant flow pumps and the sampling time of the sugar analyzer through a computer, and reads the sugar concentration in real time. concentration value.

According to the technical scheme provided by the present invention, the on-line sugar concentration detection system in the fermentation process includes a sugar concentration analyzer, the input of the sugar concentration analyzer is connected to the mixing container; the inlet of the mixing container is connected to two constant flow pumps through a pipeline At the outlet, the first constant-flow pump is connected to the fermenter to take samples from the fermenter; the second constant-flow pump continuously takes pure water, and the liquid from the two constant-flow pumps flows into the mixing container and is sent to the sugar concentration analysis after being mixed and diluted. instrument for online testing;

The sugar concentration analyzer, the first constant flow pump, and the second constant flow pump are all connected to the controller, and the controller collects the data of the sugar concentration analyzer in real time, and sets the dilution factor according to the detection concentration range of the sugar concentration analyzer, Control the operation of two constant flow pumps.

The controller regularly obtains the detection results of the sugar analyzer, and performs operations of saving, displaying, statistics, trend analysis, drawing of trend graphs, or printing reports.

The controller is preferably a computer or a single-chip microcomputer with a touch screen.

The advantages of the present invention are: the present invention integrates the constant flow pump and the sugar analyzer, the user can set various parameters of the constant flow and the sugar analyzer through the computer, control its operation status, collect the sugar concentration online, and realize the online sugar concentration display , Statistical analysis, trend chart, print report and other functions. The whole set of equipment is a self-contained system, which does not require secondary development based on the sugar analyzer, and has a DDE communication interface with other applications for easy expansion.

Description of drawings

Fig. 1 is a schematic diagram of the structure of the present invention.

Detailed ways

The present invention will be further described below in conjunction with drawings and embodiments.

As shown in Figure 1, the present invention comprises two constant-flow pumps and an online sugar concentration analyzer 5 (sugar analyzer for short). Considering that the detection range of the YSI2700 sugar concentration analyzer 5 is limited, the first constant flow pump 2 is used to take samples from the fermentation tank, the second constant flow pump 3 continuously takes pure water, and the liquid of the two constant flow pumps flows to the mixing container 4 After mixing and diluting, it is sent to YSI2700 sugar concentration analyzer 5 for online detection. The operation of the whole system device is controlled by computer, and the dilution factor can be flexibly set according to the detection concentration range of the fermentation broth, and the data can be collected in real time.

The present invention adopts the first constant flow pump 2 model TBP1010, the second constant flow pump 3 model TBP1002, and the online sugar analyzer 5 YSI2700. The lines with arrows in Figure 1 are pipelines, and other connecting lines are communication lines. The first constant flow pump 2, the second constant flow pump 3 and the YSI2700 sugar analyzer all have RS-232 serial ports, which are connected to the computer through a communication line. The inlet of the first constant flow pump 2 is connected to the fermenter, and the second constant flow pump 3 The inlet is connected to pure water, the outlets of the first constant flow pump 2 and the second constant flow pump 3 are connected to the mixing container, and YSI2700 is connected to the mixing container. In the specific implementation process, the user sets the operating parameters of the constant flow pump or the sugar analyzer 5 on the computer according to the needs, and the first constant flow pump 2 and the second constant flow pump 3 start to take out the fermentation broth and pure water respectively, and flow them to the The mixing container 4 is diluted, and the sugar analyzer 5 regularly takes the diluent from the mixing container 4 for analysis, while the computer regularly fetches the detection results of the sugar analyzer 5 .

The present invention controls the flow rate of two constant flow pumps and the sampling time of the sugar analyzer 5 through a computer. First, the computer calculates the flow rate of the two pumps according to the dilution factor set automatically or manually, and turns on the pumps to take samples and water to realize the proportional dilution of the fermentation broth, and then controls the sugar analyzer 5 to regularly sample and analyze, and read the sugar concentration in real time The value is stored in the real-time database, and functions such as display, trend analysis, and report printing are realized.

The present invention comprises following several parts:

Sugar concentration dilution (control of the constant flow pump): The sugar concentration of the fermentation broth may sometimes be high and exceed the detection range of the sugar analyzer 5. The problem of the limited detection range of the sugar analyzer 5 is solved by using two constant flow pumps. The computer adjusts the dilution factor in real time according to the actual concentration detected, and calculates the flow rate of the constant-flow pump with reference to the flow rate range of the constant-flow pump, and controls the operation of the constant-flow pump. One pump takes out the fermentation broth, one pump flows out pure water, and the liquids of the two pumps are mixed and diluted in a special container 4 .

On-line analysis of sugar concentration (control of sugar analyzer 5): through the communication between the serial port of the sugar analyzer YSI2700 and the computer, the online control of the computer is realized. The computer sets the sampling analysis time, calibration time, sampling or monitoring station and other related parameters of the sugar analyzer 5, and then regularly reads the data of the sugar analyzer 5.

Computer analysis and processing of sugar concentration values: the computer stores the collected concentration values in a real-time database, displays them in real time, draws trend graphs, conducts statistical analysis, and recalculates the dilution factor and the flow rate of the constant flow pump. There is an interface with other application programs in the computer, and the concentration value can be transmitted to other application programs to realize online control of the concentration value on the tank.

Claims (3)

1. An online sugar concentration detection system in the fermentation process, comprising a sugar concentration analyzer (5), characterized in that: the input of the sugar concentration analyzer (5) is connected to the mixing container (4); the inlet of the mixing container (4) Connect the outlets of two constant flow pumps through pipelines, the first constant flow pump (2) is connected with the fermentation tank, and takes samples from the fermentation tank; the second constant flow pump (3) continuously takes pure water, and the liquid of the two constant flow pumps After flowing into the mixing container (4) through mixing and dilution, it is sent to a sugar concentration analyzer (5) for on-line detection; The sugar concentration analyzer (5), the first constant flow pump (2), and the second constant flow pump (3) are all connected with the controller (1), and the controller (1) collects the sugar concentration analyzer ( 5), and set the dilution factor according to the detection concentration range of the sugar concentration analyzer (5), to control the operation of the two constant flow pumps. 2. The on-line sugar concentration detection system in the fermentation process according to claim 1, characterized in that the controller (1) regularly takes the detection results of the sugar analyzer (5), and saves, displays, statistics, and trend analysis , trend graph drawing, or print report operation. 3. The online sugar concentration detection system in the fermentation process according to claim 1, characterized in that the controller (1) is preferably a computer or a single-chip microcomputer with a touch screen.

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