CN101619288B - Novel biphasic culture bottle of blood samples - Google Patents
Novel biphasic culture bottle of blood samples Download PDFInfo
- Publication number
- CN101619288B CN101619288B CN 200910063598 CN200910063598A CN101619288B CN 101619288 B CN101619288 B CN 101619288B CN 200910063598 CN200910063598 CN 200910063598 CN 200910063598 A CN200910063598 A CN 200910063598A CN 101619288 B CN101619288 B CN 101619288B
- Authority
- CN
- China
- Prior art keywords
- bottle
- culture
- blood samples
- culture plate
- bottle body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004369 blood Anatomy 0.000 title claims abstract description 13
- 239000008280 blood Substances 0.000 title claims abstract description 13
- 230000002051 biphasic effect Effects 0.000 title claims abstract description 11
- 239000007787 solid Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a device for culturing blood samples, in particular to a novel biphasic culture bottle of blood samples, which comprises a bottle body, wherein a bottle neck is arranged above the bottle body. The novel bottle is characterized in that at least one culture plate is arranged in the bottle neck with a small caliber above the bottle body; both sides of the culture plate sink inward, and the sunk part is provided with a solid culture medium; the culture plate is connected with the bottom of a sealing cover; a liquid culture medium is arranged below the bottle body; and a small magnetic block is arranged at the bottom in the bottle body. The novel biphasic culture bottle of blood samples has the advantages of simple structure and convenient use and can be used for intuitively observing the culture condition of bacteria to be more convenient for picking out the bacterial colony.
Description
Technical field
The present invention relates to a kind of device that blood sample is cultivated, specifically a kind of novel biphasic culture bottle of blood samples.
Background technology
In hospital clinical and medical research test, often need patients'blood and cerebrospinal fluid equal samples are carried out microbial culture, so that find out malignant bacteria.General method is to utilize existing biphasic cultivation bottle or utilize the blood cultivation instrument to cultivate now.Because the culturing bottle that import blood cultivation instrument uses costs an arm and a leg, existing laboratory generally adopts the biphasic cultivation bottle to carry out blood cultivation.But mostly the biphasic cultivation bottle that uses is owing to be to carry out a bottle interior now, and the bottom of bottle and the both sides of bottle are substratum, and cultivation finishes the back to choosing bacterium colony inconvenience especially in the positive bottle.
Summary of the invention
Technical problem to be solved by this invention is the defective that exists at existing biphasic cultivation bottle, and a kind of novel biphasic culture bottle of blood samples is provided, and it can observe and choose the bacterium of cultivating intuitively.
Technical scheme of the present invention is achieved in that it comprises that a top is provided with the bottle of bottleneck, it is characterized in that: in the osculum bottleneck of top at least one culture plate is housed, the both sides depression of culture plate, solid medium is equipped with at its depression position, culture plate is connected with the bottom of capping, liquid nutrient medium is equipped with in the bottle below, and it is fast that the intravital bottom of bottle is provided with little magnetic; One side of described bottle has the sample mouth; The top of described capping is provided with driving handle; The both sides of described culture plate are equipped with draw-in groove, and substratum is fixed in the draw-in groove.
The present invention has advantage simple in structure, easy to use, and it can observe the cultivation situation of bacterium intuitively, the convenient bacterium colony that degerms of choosing.
Description of drawings
Fig. 1 is a structural representation of the present invention
Fig. 2 is the structural representation of culture plate of the present invention
Fig. 3 is the structural representation of culture plate draw-in groove of the present invention
Embodiment
As shown in Figure 1 and Figure 2, the present invention includes a top and be provided with the bottle 9 of bottleneck 8, in the top osculum bottleneck 8 at least one culture plate 2 is housed, the both sides depression of culture plate 2, solid medium 7 is equipped with at its depression position, and culture plate 2 is connected with the bottom of capping 3, capping 3 is fixed on the bottleneck 8 by screw thread, liquid nutrient medium 5 is equipped with in bottle below, and it is fast 6 that the intravital bottom of bottle is provided with little magnetic, mixes after being convenient to put it into alternating magnetic field; One side of described bottle has sample mouth 4; The top of described capping 3 is provided with driving handle 1, is convenient to culturing bottle is stirred.As shown in Figure 3, the both sides of described culture plate 2 are equipped with draw-in groove 10, and substratum 5 is fixed in the draw-in groove 10, can prevent that like this substratum 5 from coming off.
Claims (1)
1. novel biphasic culture bottle of blood samples, it comprises that a top is provided with the bottle of bottleneck, it is characterized in that: in the osculum bottleneck of top at least one culture plate is housed, the both sides depression of culture plate, solid medium is equipped with at its depression position, the both sides of culture plate are equipped with draw-in groove, substratum is fixed in the draw-in groove, and culture plate is connected with the bottom of capping, and liquid nutrient medium is equipped with in the bottle below, the intravital bottom of bottle is provided with little magnetic patch, and a side of bottle has the sample mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910063598 CN101619288B (en) | 2009-08-11 | 2009-08-11 | Novel biphasic culture bottle of blood samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910063598 CN101619288B (en) | 2009-08-11 | 2009-08-11 | Novel biphasic culture bottle of blood samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101619288A CN101619288A (en) | 2010-01-06 |
| CN101619288B true CN101619288B (en) | 2011-12-21 |
Family
ID=41512701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910063598 Active CN101619288B (en) | 2009-08-11 | 2009-08-11 | Novel biphasic culture bottle of blood samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101619288B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845390B (en) * | 2010-04-30 | 2013-09-11 | 武汉迪艾斯科技有限公司 | Automatic bacteria culture instrument |
| CN109055184B (en) * | 2018-09-04 | 2024-04-12 | 武汉迪艾斯科技有限公司 | Automatic seed turning device that changes of diphasic blood blake bottle |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4840908A (en) * | 1988-06-29 | 1989-06-20 | Becton, Dickinson And Company | Culture bottle assembly |
| US4952510A (en) * | 1987-01-02 | 1990-08-28 | Bio-North, Inc. | Apparatus for detecting and culturing microorganisms using a biphasic culture vessel |
| CN2526847Y (en) * | 2002-03-15 | 2002-12-18 | 中国人民解放军第二军医大学 | Double phase blood culturing bottle |
| CN2563160Y (en) * | 2002-08-29 | 2003-07-30 | 陈寅初 | Double phase culture bottle |
| CN201574151U (en) * | 2009-03-13 | 2010-09-08 | 武汉迪艾斯科技有限公司 | Novel blood sample biphasic culture bottle |
-
2009
- 2009-08-11 CN CN 200910063598 patent/CN101619288B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4952510A (en) * | 1987-01-02 | 1990-08-28 | Bio-North, Inc. | Apparatus for detecting and culturing microorganisms using a biphasic culture vessel |
| US4840908A (en) * | 1988-06-29 | 1989-06-20 | Becton, Dickinson And Company | Culture bottle assembly |
| CN2526847Y (en) * | 2002-03-15 | 2002-12-18 | 中国人民解放军第二军医大学 | Double phase blood culturing bottle |
| CN2563160Y (en) * | 2002-08-29 | 2003-07-30 | 陈寅初 | Double phase culture bottle |
| CN201574151U (en) * | 2009-03-13 | 2010-09-08 | 武汉迪艾斯科技有限公司 | Novel blood sample biphasic culture bottle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101619288A (en) | 2010-01-06 |
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