CN101576554B - Dynamic running water type toxicity test device of fishes - Google Patents
Dynamic running water type toxicity test device of fishes Download PDFInfo
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- CN101576554B CN101576554B CN 200910079544 CN200910079544A CN101576554B CN 101576554 B CN101576554 B CN 101576554B CN 200910079544 CN200910079544 CN 200910079544 CN 200910079544 A CN200910079544 A CN 200910079544A CN 101576554 B CN101576554 B CN 101576554B
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 92
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 87
- 231100000820 toxicity test Toxicity 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 54
- 239000003814 drug Substances 0.000 claims abstract description 42
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 238000005286 illumination Methods 0.000 claims description 10
- 238000006213 oxygenation reaction Methods 0.000 claims description 9
- 230000000875 corresponding effect Effects 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 238000003760 magnetic stirring Methods 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000004088 simulation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
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- 230000002596 correlated effect Effects 0.000 claims description 2
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- 238000011047 acute toxicity test Methods 0.000 description 1
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Abstract
The invention relates to a dynamic running water type toxicity test device of fishes. The invention is characterized in that the test device is a frame which is internally provided with a plurality offish tanks; each fish tank is respectively matched with a corresponding drug feeding container and a drug feeding peristaltic pump; the drug feeding container, the fish tank and the drug feeding peristaltic pump are connected mutually by drug conveying pipes; each fish tank is also respectively matched with a corresponding circulating water tank and a circulating water pump; and the fish tank, the circulating water tank and the circulating water pump are connected mutually by liquid conveying pipes.
Description
Technical field
The present invention relates to a kind of dynamic running water type toxicity test device of fishes.Equipment is controlled simply, can guarantee reliably carrying out and finishing of experiment.
Background technology
At present a large amount of industrial excretas and discharge of wastewater cause river and lake pollution in wide area in river and lake, river Mesichthyes life is subject to serious threat, and sewage also threatens the safety of drinking water source, downstream.Although present many factories all carry out wastewater treatment before waste discharge, but still there is certain toxicant in the sewage after processing, fish are still caused toxicity, regrettably now also do not have a kind of device of measuring flow-type river Mesichthyes toxicity that is directly used in.
Summary of the invention
For overcoming the defects of prior art, the invention provides a kind of simulation dynamic flow water fish class toxicity test device.This device can be regulated the residing temperature environment of the fish in the fish jar and water environment, the flow velocity of fish jar dosing and water is all adjustable, simulate effect to physical environment is obvious, and the dynamic acute and chronic toxicity test detection system of the continuous-flow type river Mesichthyes that can substantially open current, and related streams ability of swimming water environment Mesichthyes is accumulated or experimental study on degradation.
The present invention realizes that the technical scheme of above-mentioned purpose is: a kind of dynamic running water type toxicity test device of fishes, it is characterized in that described test unit is a framework, comprise several fish jars in the described framework, what described each fish jar all was furnished with a correspondence adds medicine container and dosing peristaltic pump, described fish jar, describedly add medicine container and described dosing peristaltic pump interconnects by the medicine delivery pipe, described each fish jar also all is furnished with circulation water vat and the water circulating pump of a correspondence, described fish jar, described circulation water vat and described water circulating pump interconnect by liquid delivery tube, for test provides accurately consistent test condition and environment.
Described water circulating pump constantly extracts water according to the water velocity of test needs from described fish jar, then water is sent into described circulation cylinder, water flows into described fish jar again from described circulation cylinder subsequently, forms continuous circulating water flow, has finished the simulation of dynamic water environment.
Described dosing peristaltic pump constantly extracts medicine from described adding according to the dosing speed of test needs the medicine container, again evenly mix and then described medicine is sent into described corresponding fish jar.
Described fish jar places on the temperature-controlling system, regulates temperature-controlling system, the temperature that provides test to need.
Described test unit is furnished with illumination system and oxygenation apparatus, and described oxygenation apparatus constantly provides enough oxygen for the fish in the described fish jar.
Description of drawings
Fig. 1 is structural representation of the present invention;
Fig. 2 is elevational schematic view of the present invention.
Embodiment
Referring to Fig. 1, the invention provides a kind of dynamic running water type toxicity test device of fishes, mainly comprise illumination system 1, circulation water vat 2, water circulating pump 3, dosing peristaltic pump 4, oxygenation apparatus 5, add medicine container 7, magnetic stirring apparatus 8, fish jar 9, temperature-controlling system 10, described fish jar is provided with several flow control mouths 13.
Be provided with several fish jars in the described test unit, described per three fish jars are a parallel experiment group, in the described parallel experiment group, a described fish jar is blank test, described other two fish jars are the dosing test, at least establish two described parallel experiment groups in the described test unit, described test unit can need to increase a plurality of described parallel experiment groups according to actual tests simultaneously.
The actual tests water capacity of described fish jar can hold 10 less than the test fish of 4 grams at least greater than 5L, each test group 〉=10 fish, and the bad behavior of fish or mortality ratio should be controlled in 10%.
Described each fish jar all is furnished with water circulating pump and the circulation water vat of a correspondence, described fish jar, described water circulating pump and described circulation water vat are connected successively by liquid delivery tube, described water circulating pump constantly extracts water according to the water velocity of test needs from described fish jar, then water is sent into described circulation cylinder, water flows into described fish jar again from described circulation cylinder subsequently, form continuous circulating water flow, described circulating water flow has been simulated dynamic water environment.
Described test water is general to be adopted through dechlorination and the softening filtration tap water of processing or to add some mineral elements in pure water formulated, and main index is: pH=6.5-8.5, dissolved oxygen DO＞5.0, conductivity=200-400 μ Ω/cm.
Volume＞the 2.5L of described circulation cylinder, the speed of current can be controlled at 0.1-100ml/s, the flow of water circulating pump should be 1-100ml/min, in addition, the volume of circulation cylinder and the flow parameter of water circulating pump can according to the in proportion corresponding increasing of volume of fish jar, carry out the check test of the toxicologic index of larger fish.
The medicine that adds in the described fish jar at first dissolves, mixes in described adding in the medicine container, be close to described each bottom that adds medicine container and be equipped with magneton, described each add medicine container followingly all be equipped with a corresponding magnetic stirring apparatus, start described magnetic stirring apparatus, magneton is done up-down vibration and forward and reverse rotation, the described medicine that adds in the medicine container is evenly mixed, and assurance adds the chemical substance dissolving in the medicine container and is evenly distributed.
The medicine that adds in the described fish jar should can directly dissolve or can be dissolved in fully the inorganic or organic chemicals in the test water after the cosolvent effect in the toxicology test concentration range that requires, described chemical substance concentration scope is usually less than 1000mg/L, the concentration change of chemical substance is controlled at ± 20% scope in.
Described dosing peristaltic pump constantly extracts medicine from described adding according to the dosing speed of test needs the medicine container, again evenly mix and then described medicine is sent into described corresponding fish jar.Described fish jar dosing speed can be controlled at 0.1-100ml/s.
Described fish jar places above the temperature-controlling system, described temperature-controlling system comprises temperature control tank, temperature controller, well heater 11 and sensor 12, described sensor, described well heater and the temperature controller of being connected connect by signal wire, sensor measurement goes out the temperature in the temperature control tank, then temperature signal is transferred to temperature controller, described temperature controller is according to the duty of described temperature control signal controlling well heater, the living environment temperature that needs is provided for the fish in the fish jar, the temperature range of described water can be room temperature-40 ℃, temperature fluctuates and should be controlled at ± and 0.1-0.3 ℃.
Described battery of tests fish jar is positioned in the temperature-controlling system.
Be provided with illumination system and oxygenation apparatus in the described test unit housing, described illumination system generally adopts daylight lamp or flash bulb, can regulate the test unit irradiation temperature according to physical environment, the intensity of illumination scope can be 100-20000LX, described oxygenation apparatus can be one or several corresponding with described fish jar, described oxygenation apparatus links to each other with described fish jar by air shooter, constantly provides enough oxygen for the fish in the described fish jar.
Described test unit inner housing divides some layers, is used for placing described several fish jars of layered mode, adding medicine container, dosing peristaltic pump, and described medicine container, circulation water vat and the water circulating pump of adding, layer separates with dividing plate with interlayer, and described dividing plate is played a supporting role; Described device frame bottom is provided with bracing frame, and support frame as described above contacts with ground, and test unit is played a supportive role.
The material that described test unit framework, assembly and connection tubing are connected should meet correlated quality control and test the requirement of using stores management, should be the harmless experiment of corrosion resistant, antiacid alkali and dimensionally stable steel, glass, pottery or high-molecular organic material etc.; As adopt stainless steel to make framework, and glass is made test fish jar and test cycle cylinder, and glass or stiff stability macromolecular material are made heating cylinder groove etc.
The test facilities spatial dimension of described test unit can be 5-100m
2Perhaps larger, applicable power supply is 220V/50HZ, and environment temperature can be 5-45 ℃.
The dynamic acute toxicity test of fish or the fish dynamic running water type chronic toxicity toxicology test of longer time that described test unit can carry out 24-96h detect.
Described test will be furnished with plumbing interface and the use in fish breeding water processing establishment of coupling.
The advantage of this test test unit is:
(1) adopts water circulating pump and circulation cylinder, water circulating pump is according to the water in the water velocity extraction fish jar of setting, flow to circulation cylinder, then current flow back to fish jar again from circulation cylinder, realized water circuit in the fish jar, reach the effect of dynamic flowing water, effectively simulated the continuous-flow type fluvial-environment that current are opened substantially.
(2) employing adds medicine container, dosing peristaltic pump and magnetic stirring apparatus, medicine mixes in adding medicine container, dissolves, effect through magnetic stirring apparatus, medicine mixes more even, the dosing peristaltic pump extracts liquid according to the dosing speed of test needs from add medicine container, further medicine is mixed, and is transported to subsequently in the fish jar, effectively simulated the dosing situation that current are opened substantially, and dosing speed can be regulated according to actual conditions and test needs.
(3) can regulate according to actual conditions and test needs because of water velocity and dosing speed, so regulate water velocity and dosing speed, can finish the dynamic acute and chronic toxicity test detection system of the continuous-flow type river Mesichthyes that current open substantially, and related streams ability of swimming water environment Mesichthyes is accumulated or experimental study on degradation.
(4) test unit all is furnished with a dosing fish jar and dosing peristaltic pump by each fish jar, each fish jar all is furnished with a circulation water vat and water circulating pump, each fish jar all is connected with the oxygen of same airflow speed, in the temperature control tank that the fish jar of identical test group is positioned over same temperature and the identical intensity of illumination environment, for test provides accurate consistent test condition and experimental enviroment.
(5) by closing or do not start the device of circulating water flow, can finish the research of Static Water Mesichthyes toxicity test.
(6) be provided with temperature-controlling system and illumination system in the device, provide required temperature environment by regulating temperature-controlling system for test, provide required photoenvironment by capable of regulating illumination intensity for test, thereby guaranteed the accurate simulation of continuous-flow type fluvial-environment that current are opened substantially.
Claims (7)
1. dynamic running water type toxicity test device of fishes, it is characterized in that described test unit is a framework, comprise several fish jars in the described framework, what described each fish jar all was furnished with a correspondence adds medicine container and dosing peristaltic pump, the described medicine container that adds, described fish jar and described dosing peristaltic pump interconnect by the medicine delivery pipe, described each fish jar also all is furnished with circulation water vat and the water circulating pump of a correspondence, described fish jar, described circulation water vat and described water circulating pump interconnect by liquid delivery tube, described dosing peristaltic pump constantly extracts medicine from described adding according to the dosing speed of test needs the medicine container, again evenly mix and then described medicine is sent into described corresponding fish jar, described fish jar is provided with several flow control mouths, described water circulating pump constantly extracts water according to the water velocity of test needs from described fish jar, then water is sent into described circulation cylinder, water flows into described fish jar again from described circulation cylinder subsequently, form continuous circulating water flow, described circulating water flow has been finished the simulation of dynamic water environment, medicine dissolved in described adding in the medicine container, mix, be close to described each bottom that adds medicine container and be equipped with magneton, described each add medicine container followingly all be equipped with a corresponding magnetic stirring apparatus.
2. described dynamic running water type toxicity test device of fishes according to claim 1, it is characterized in that described fish jar places above the temperature-controlling system, described temperature-controlling system comprises temperature control tank, temperature controller, well heater and sensor, described sensor, described well heater and the temperature controller of being connected connect by signal wire, regulate described temperature-controlling system, provide fish jar needed environment temperature.
3. described dynamic running water type toxicity test device of fishes according to claim 1 is characterized in that being provided with illumination system and oxygenation apparatus in the test unit housing, and the bottom of housing is provided with bracing frame, and support frame as described above contacts with ground.
4. described dynamic running water type toxicity test device of fishes according to claim 3, it is characterized in that described oxygenation apparatus is one or several corresponding with described fish jar, described oxygenation apparatus links to each other with described fish jar by air shooter, constantly provides enough oxygen for the fish in the described fish jar.
5. described dynamic running water type toxicity test device of fishes according to claim 1, it is characterized in that described test unit inner housing divides some layers, being used for layering places described several fish jars, adds medicine container, dosing peristaltic pump, circulation water vat and water circulating pump, layer separates with dividing plate with interlayer, and described dividing plate is played a supporting role.
6. according to claim 1,2,3,4 or 5 described dynamic running water type toxicity test device of fishes, it is characterized in that fish jar dosing and water velocity scope are at 0.1-100ml/s, illumination is controlled at 100-20000LX, water temperature is controlled at room temperature--and 40 ℃ ± 0.1-0.3 ℃, test water leading indicator: PH=6.5-8.5, dissolved oxygen DO＞5.0, conductivity=200-400u Ω/cm.
7. described dynamic running water type toxicity test device of fishes according to claim 6, it is characterized in that the material that described test unit framework, assembly and connection tubing are connected should meet correlated quality control and test the requirement of using stores management, should be the harmless experiment of corrosion resistant, antiacid alkali and dimensionally stable steel, glass, pottery or high-molecular organic material.
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| CN 200910079544 CN101576554B (en) | 2009-03-06 | 2009-03-06 | Dynamic running water type toxicity test device of fishes |
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| CN 200910079544 CN101576554B (en) | 2009-03-06 | 2009-03-06 | Dynamic running water type toxicity test device of fishes |
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| CN101576554A CN101576554A (en) | 2009-11-11 |
| CN101576554B true CN101576554B (en) | 2013-10-16 |
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| CN 200910079544 Expired - Fee Related CN101576554B (en) | 2009-03-06 | 2009-03-06 | Dynamic running water type toxicity test device of fishes |
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