CN101569759B - Isotonic collagen sponge and preparation method thereof - Google Patents
Isotonic collagen sponge and preparation method thereof Download PDFInfo
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- CN101569759B CN101569759B CN200910068412A CN200910068412A CN101569759B CN 101569759 B CN101569759 B CN 101569759B CN 200910068412 A CN200910068412 A CN 200910068412A CN 200910068412 A CN200910068412 A CN 200910068412A CN 101569759 B CN101569759 B CN 101569759B
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- collagen
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- 239000000515 collagen sponge Substances 0.000 title claims abstract description 7
- 238000002360 preparation method Methods 0.000 title claims description 3
- 102000008186 Collagen Human genes 0.000 claims abstract description 28
- 108010035532 Collagen Proteins 0.000 claims abstract description 28
- 229920001436 collagen Polymers 0.000 claims abstract description 27
- 150000003839 salts Chemical class 0.000 claims abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000000502 dialysis Methods 0.000 claims description 10
- 210000003722 extracellular fluid Anatomy 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000005360 mashing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 210000001519 tissue Anatomy 0.000 claims description 5
- 210000001124 body fluid Anatomy 0.000 claims description 4
- 239000010839 body fluid Substances 0.000 claims description 4
- 230000003204 osmotic effect Effects 0.000 claims description 3
- 208000035126 Facies Diseases 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 208000037976 chronic inflammation Diseases 0.000 abstract description 2
- 239000012620 biological material Substances 0.000 abstract 1
- 230000006020 chronic inflammation Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 208000037893 chronic inflammatory disorder Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to an isotonic collagen sponge product, provides a material which can be clinically used for emergently stanching the surface of wound, filling a residual cavity and treating chronic inflammation and belongs to the field of medical biomaterials. The invention aims at solving the problem of poor flexibility and cosiness of the currently clinically used collagen protein sponge. By introducing physiological salt which has the similar concentration with the human body environment into collagen, the invention improves the adhesiveness of tissues and the product as well as the flexibility thereof.
Description
Technical field
The present invention relates to a kind of and the body fluid environment of human body has and equates or the collagen sponge product of similar osmotic pressure.
Background technology
Collagen sponge has been widely used in the treatment of acute wound surface hemostasis, residual cavity filling and chronic inflammatory disease in the clinical operation.But because the particularity of preparation causes collagen in clinical, to have the problem aspect two with itself structure: the one, the adhesion of product itself is not enough, usually occurs departing from wound site in the operation, or receives hemorrhage the influence and the phenomenon that comes off; The 2nd, product is harder, and during filling, it is uncomfortable that the patient feels; The present invention bases oneself upon the deficiency of improving collagen, contains in the prepared collagen and physiology salt like the human body environment facies, oozes effect with body fluid formation etc. in use; Adhesiveness is able to strengthen, and owing to the existence of NaCl, the stretched out structure of collagen fiber is able to change simultaneously; Therefore, its pliability obtains embodying.Thereby obtain clinical middle satisfied product.
Summary of the invention
The purpose of this invention is to provide a kind of and human body fluid environment has and equates or the collagen sponge product of similar osmotic pressure.Its step is following:
1, selecting the thing of saltouing in commercially available collagen or the self-control collagen process is raw material.
2, commercially available collagen adds 0.1mol/L HCl solution, makes collagen quality volume by volume concentration reach 0.2～0.8%, becomes collagen solution with tissue mashing machine's homogenate.
3, adjust collagen solution pH value to 9～11 with the NaOH solution of 10mol/L.
4, the solution in 3 or the self-control collagen thing of saltouing is packed into and dialysed in the bag filter, extracellular fluid dialysis is the NaCl solution of 100～150mmol/L, changes extracellular fluid dialysis one time, dialyses 5 days in per 4 hours.
5, taking out dialysate, to add the mass and size specific concentration be that water to the collagen quality volume by volume concentration that adds with volume was 0.6～1.4%, stirs the lyophilizing of dress mould after 0.1% acetic acid was diluted to collagen quality volume by volume concentration and is 1.2～2.8%.
The specific embodiment
Embodiment one
Commercially available collagen 20g adds 0.1mol/L HCl solution 4000ml, with the homogenate 5min of tissue mashing machine.With the NaOH solution of 10mol/L adjustment collagen solution pH value to 9.0, solution to be packed into dialyse in the bag filter, extracellular fluid dialysis is the NaCl solution of 100mmol/L, changes extracellular fluid dialysis one time, dialyses 5 days in per 4 hours.Dialysate adding mass and size specific concentration is 0.1% acetic acid 900ml, and thin up stirs to 1800ml, the lyophilizing of dress mould.
Embodiment two
Commercially available collagen 20g adds 0.1mol/L HCl solution 10000ml, with the homogenate 5min of tissue mashing machine.With the NaOH solution of 10mol/L adjustment collagen solution pH value to 10, solution to be packed into dialyse in the bag filter, extracellular fluid dialysis is the NaCl solution of 130mmol/L, changes extracellular fluid dialysis one time, dialyses 5 days in per 4 hours.It is 0.1% acetic acid 1700ml that dialysate adds the mass and size specific concentration, and thin up stirs to 3400ml, the lyophilizing of dress mould.
Embodiment three
The self-control collagen thing 20g that saltouts adds 0.1mol/L HCl solution 2500ml, with the homogenate 5min of tissue mashing machine.With the NaOH solution of 10mol/L adjustment collagen solution pH value to 11, solution to be packed into dialyse in the bag filter, extracellular fluid dialysis is the NaCl solution of 150mmol/L, changes extracellular fluid dialysis one time, dialyses 5 days in per 4 hours.It is 0.1% acetic acid 700ml that dialysate adds the mass and size specific concentration, and thin up stirs to 1400ml, the lyophilizing of dress mould.
Claims (1)
1. one kind has with the body fluid environment of human body and to equate or the collagen sponge of similar osmotic pressure, wherein contains and physiological salt concentration like the human body environment facies, it is characterized in that the preparation process comprises following each step:
(1) a certain amount of commercially available collagen adds in the 0.1mol/L HCl solution, becomes collagen solution with tissue mashing machine's homogenate, is mixed with the mass and size specific concentration and is 0.2～0.8% collagen solution;
(2) adjust collagen solution pH value to 9～11 with the NaOH solution of 10mol/L;
(3) collagen solution that step (2) is made is packed into, and bag filter is interior dialyses, and extracellular fluid dialysis is the NaCl solution of 100～150mmol/L, changes extracellular fluid dialysis one time, dialyses 5 days in per 4 hours;
(4) taking out dialysate, to add the mass and size specific concentration be that water to the collagen quality volume by volume concentration that adds with volume was 0.6～1.4%, stirs the lyophilizing of dress mould after 0.1% acetic acid was diluted to collagen quality volume by volume concentration and is 1.2～2.8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910068412A CN101569759B (en) | 2009-04-10 | 2009-04-10 | Isotonic collagen sponge and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910068412A CN101569759B (en) | 2009-04-10 | 2009-04-10 | Isotonic collagen sponge and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101569759A CN101569759A (en) | 2009-11-04 |
| CN101569759B true CN101569759B (en) | 2012-09-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910068412A Expired - Fee Related CN101569759B (en) | 2009-04-10 | 2009-04-10 | Isotonic collagen sponge and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101569759B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584150A (en) * | 2004-05-28 | 2005-02-23 | 四川大学 | Preparing method for collagen stroma composite medical fibre spinning stoste |
| EP1593399A2 (en) * | 2004-05-07 | 2005-11-09 | Resorba Wundversorgung GmbH & Co. KG | Bioresorbable wound dressing based on collagen |
| CN1915437A (en) * | 2005-08-15 | 2007-02-21 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
-
2009
- 2009-04-10 CN CN200910068412A patent/CN101569759B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1593399A2 (en) * | 2004-05-07 | 2005-11-09 | Resorba Wundversorgung GmbH & Co. KG | Bioresorbable wound dressing based on collagen |
| CN1584150A (en) * | 2004-05-28 | 2005-02-23 | 四川大学 | Preparing method for collagen stroma composite medical fibre spinning stoste |
| CN1915437A (en) * | 2005-08-15 | 2007-02-21 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
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| Publication number | Publication date |
|---|---|
| CN101569759A (en) | 2009-11-04 |
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Owner name: TIANJIN CITY SAINING BIOLOGICAL ENGINEERING TECHNO Free format text: FORMER OWNER: TIANJIN BEIYI BIOLOGICAL PRODUCTS CO., LTD. Effective date: 20130206 |
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Free format text: CORRECT: ADDRESS; FROM: 301609 JINGHAI, TIANJIN TO: 300384 NANKAI, TIANJIN |
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Effective date of registration: 20130206 Address after: The 300384 Tianjin City Huayuan Industrial Zone Development Road six No. 6 Haitai green industry base building F room 7-302 Patentee after: TIANJIN SANNIE BIOENGINEERING TECHNOLOGY Co.,Ltd. Address before: 301609 Tianyu science and Technology Park, Tianjin New Technology Industrial Park, Jinghai, Tianjin Patentee before: TIANJIN BEIYI BIOLOGICAL PRODUCTS Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |