CN101564032B - Female mosquito olfaction disrupter composition, preparation method and applications thereof - Google Patents

Abstract

A female mosquito olfactory disruptor composition and its preparation method and application belong to the technical field of household chemicals. It is characterized in that it contains 0.1-9.9% of the total weight of organic acid as an effective component, and contains 88-99.6% of total weight of water as a solvent. The organic acid is acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid , n-hexanoic acid, n-heptanoic acid, L-lactic acid, malic acid or a mixture of more than one. The composition simulates the smell of human skin and breath, and prevents people from being bitten by mosquitoes by interfering with the sense of smell of mosquitoes, and has a good effect of preventing mosquitoes from biting people; the raw materials are all in accordance with food-grade standards, and the prepared composition is safe to humans; it has no killing effect on mosquitoes. Extinction, there is no resistance problem, and there is no ecological risk. Moreover, the preparation method is simple, the production cost is low, and there are no three wastes in the production process. It is used to prepare products for preventing mosquito bites, products for relieving itching and detumescence after mosquito bites, and products for air sterilization and disinfection and air cleaning.

Description

A method for interfering with female mosquitoes' sense of smell

technical field

The invention belongs to the technical field of household chemicals, and specifically relates to a composition of a female mosquito olfactory disruptor, a preparation method and application thereof. the

Background technique

Mosquito bites and sucks blood not only makes people itchy, but also transmits pathogenic bacteria to people. Generally speaking, male mosquitoes will not bite people, only female mosquitoes will bite people. Studies have shown that mosquitoes mainly rely on the unique smell emitted by the target to find the target at a long distance. This smell is the smell of human sweat. Through the analysis and research on the composition of sweat, it is found that mosquitoes rely on the identification of organic acids in sweat to determine The location of the target because of the presence of organic acid receptor proteins in the mosquito's olfactory organs. These organic acids include lactic acid, which can cause mosquitoes to be excited, but high concentrations of short-chain organic acids such as acetic acid, propionic acid, butyric acid, etc. can inhibit the excitement of mosquitoes, making mosquitoes inert to look for sucking targets and not want to seek human bites For humans, low-concentration short-chain organic acids can stimulate the excitability of mosquitoes. If the living environment is permeated with low-concentration short-chain organic acids, it can continuously stimulate the excitability of mosquitoes, making mosquitoes feel like the whole space is human. Covering the lactic acid odor of human sweat to stimulate the excitability of mosquitoes, so as to disturb the olfactory function of mosquitoes and make mosquitoes unable to find the target. the

Early research showed that mosquitoes can also rely on the carbon dioxide emitted by the target to find the target. However, later research has shown that this is a misunderstanding, and it is impossible for mosquitoes to rely on carbon dioxide to determine the target. Current research shows that female mosquitoes can rely on other odors emitted by human breath (non-carbon dioxide, the smell of half-digested food in the stomach) to find their bite targets. In addition, mosquitoes can also rely on the temperature and humidity of the human body to find targets. the

The active ingredients of traditional mosquito coils are pesticides, most of which are carbamate and pyrethrin insecticides, which belong to acetylcholinesterase (AChE) inhibitors and sodium channel regulators, act on the nervous system of animals, and are neurotoxins. It is to poison or kill mosquitoes so that mosquitoes cannot bite people, which has certain harm to human health. With long-term use and accumulation, it has great side effects on people. Mosquitoes are a chain in the ecological food chain, excessive killing will have a certain adverse effect on the ecological environment. In addition, with the long-term use of large quantities of pyrethroid pesticides, mosquitoes are prone to be susceptible to pyrethroid pesticides due to their greater reproductive capacity. It can be seen that killing mosquitoes is not a long-term effective way to produce resistance, but interfering with mosquitoes without biting people and killing mosquitoes is the most ecological control measure. the

Although, there are currently products on the market that contain information about human sweat, such as DEET, which is volatile and contains human sweat and breath, and works by blocking 1-octen-3-ol of mosquito olfactory receptors. Effectively depriving insects of their sense of smell from humans or animals, but without affecting the mosquito's ability to smell human breath. Recent scientific research shows that DEET, as a mosquito repellent, works by making mosquitoes feel uncomfortable, but DEET can only reduce the chance of mosquito bites, and the effect is not very good. In addition, it itself Also has certain toxicity, is not very safe to human body. the

Contents of the invention

In view of the above-mentioned problems existing in the prior art, the object of the present invention is to design and provide a technical scheme of a female mosquito olfactory disruptor composition and its preparation method and application. The preparation method is simple, there are no three wastes in the production process, and the production cost is low. , for personal safety, and has a good effect in preventing mosquitoes from biting people. the

The composition of a female mosquito olfactory disruptor is characterized in that it contains 0.1-9.9% of the total weight of organic acid as an effective component, and contains 88-99.6% of the total weight of water as a solvent. The organic acid is acetic acid, propane Acid, n-butyric acid, isobutyric acid, n-valeric acid, n-hexanoic acid, n-heptanoic acid, L-lactic acid, malic acid or a mixture of more than one. the

The composition of a female mosquito olfactory disruptor is characterized in that the content of organic acid is 1.0-8.0%, preferably 2.0-6.0%. the

The composition of a female mosquito olfactory disruptor is characterized in that the solvent is distilled water with a content of 90-98%, preferably 93-96%. the

The composition of a female mosquito olfactory disruptor is characterized in that the organic acid is a food-grade raw material. the

The composition of a female mosquito olfactory disruptor is characterized in that it also contains 0.1-0.5% of the total weight of sodium chloride or citric acid as a preservative. the

The composition of a female mosquito olfactory disruptor is characterized in that it also contains 0.1-0.5% of the total weight of water-soluble fruit and vegetable flavoring as a flavoring agent. the

The preparation method of a female mosquito olfactory disruptor composition is characterized in that: add water and an organic acid into a heating mixer according to the above ratio, the temperature of the prepared product is 15-35 °C, and stir until it is completely dissolved to obtain the product . the

The preparation method of the described a female mosquito olfactory disruptor composition is characterized in that: adding distilled water and organic acid into a heating mixer according to the above ratio, stirring and dissolving, then adding preservatives and flavorings in turn, and stirring until completely dissolved to obtain Final product. the

The preparation method of a female mosquito olfactory disruptor composition is characterized in that: the temperature of the prepared product is 20-30°C, preferably 20-25°C. the

The composition of a female mosquito olfactory disruptor is characterized in that the composition is used in the preparation of products for preventing mosquito bites, products for relieving itching and detumescence after mosquito bites, and for preparing air sterilization and air cleaning products. the

The above-mentioned female mosquito olfactory disruptor composition generally volatilizes the liquid into a gas through natural volatilization, blowing volatilization or electric heating volatilization; it can also continuously adjust the humidity in the air through the traditional heating method of electric mosquito repellent incense device. A temperature zone that is the same as the temperature of the human body is formed around, and the two factors of temperature and humidity further interfere with the mosquito's receptors, so that the mosquito cannot find the target. the

The above-mentioned female mosquito olfactory disruptor composition and its preparation method and application, the composition simulates human skin odor and breath odor, prevents people from being bitten by mosquitoes by interfering with mosquito sense of smell, and has a good effect in preventing mosquito bites; the raw materials are all in accordance with food Grade standard, the prepared composition is safe for human body, especially suitable for special populations with weak resistance such as infants, pregnant women, children and the elderly; it is not lethal to mosquitoes, there is no resistance problem, and there is no ecological risk. Moreover, the preparation method is simple, the production cost is low, and there are no three wastes in the production process, which is beneficial to environmental protection. It can also be used to prepare products for preventing mosquito bites, products for relieving itching and detumescence after mosquito bites, as well as products for air sterilization and disinfection and air cleaning, and has a wide range of uses. the

Parts and percentages involved in this patent application document are all weight units unless otherwise specified. The equipment and raw materials used can be purchased from the market or commonly used in this industry. the

Detailed ways

Below in conjunction with specific embodiment and corresponding test, the present invention will be further described. the

Embodiment 1: drop into 90.0kg distilled water in the 100L enamel stirred tank, then slowly drop into 9.9kg (purity greater than 99.9%) edible grade acetic acid, after stirring and dissolving at 20 ℃, then slowly drop into 0.1kg citric acid, stir After dissolving, it becomes the final product. the

Embodiment 2: drop into 99.6kg distilled water in the 100L enamel stirred tank, then slowly drop into 0.1kg of food grade propionic acid with a purity of 99.8%, stir and dissolve at 15°C, then slowly drop into 0.2kg of sodium chloride, stir After dissolving, slowly add 0.1kg of water-soluble apple-flavored spice, stir and dissolve to obtain the final product. the

Embodiment 3: drop into 98.5kg distilled water in the 100L enamel stirred kettle, then slowly drop into 0.5kg edible grade acetic acid and 0.5kg edible grade malic acid, after stirring and dissolving at 25°C, slowly drop into 0.3kg sodium chloride again, After stirring and dissolving, slowly add 0.2kg of water-soluble lemon-flavored spice, stir and dissolve to obtain the final product. the

Embodiment 4: drop into 91.3kg distilled water in 100L enamel stirred tank, then slowly drop into 6.0kg edible grade propionic acid, 0.5kg edible grade acetic acid, 0.5kg edible grade n-butyric acid; 0.5kg edible grade isobutyric acid, 0.5kg Food grade n-valeric acid, stirred and dissolved at 25°C, then slowly added 0.4kg of sodium chloride, stirred and dissolved, then slowly added 0.3kg of water-soluble lemon-flavored spice, stirred and dissolved to obtain the final product. the

Example 5: 97.1kg of distilled water was dropped into a 100L enamel stirred tank, and then slowly dropped into 0.9kg of n-butyric acid, 0.9kg of isobutyric acid, 0.1kg of n-hexanoic acid, and 0.1kg of n-heptanoic acid. After stirring and dissolving at 30°C, Slowly drop in 0.5kg of sodium chloride, stir and dissolve, then slowly drop in 0.4kg of water-soluble orange-flavored spice, stir and dissolve to obtain the final product. the

Embodiment 6: drop into 93kg distilled water in the 100L enamel stirred kettle, then slowly drop into 2kg n-pentanoic acid, 2kg n-hexanoic acid, 2kg n-heptanoic acid, after stirring and dissolving at 35°C, then slowly drop into 0.5kg citric acid, stir After dissolving, slowly add 0.5kg of water-soluble watermelon-flavored spice, stir and dissolve to obtain the final product. the

Embodiment 7: drop into 95kg tap water in the 100L enamel stirred kettle, then slowly drop into 3kg acetic acid, 0.2kg propionic acid, 0.2kg n-butyric acid, 0.2kg isobutyric acid, 0.2kg n-valeric acid, 0.2kg n-hexanoic acid, 0.2 kg of n-heptanoic acid, 0.2kg of L-lactic acid, and 0.6kg of malic acid are stirred and dissolved at 25°C to form the final product. the

The invention is a female mosquito olfactory disruptor, which has good safety to humans. In order to further reduce the adverse effect of the agent on human health, the addition of artificially synthesized chemicals such as spices can be reduced as much as possible during the preparation process. In addition, considering that the composition of the present invention is a liquid, the use process is to volatilize the liquid into a gas, in order to reduce the solid residue of the product after volatilization, and reduce the blockage of the water absorption channel of the liquid component by the solid component in the volatilization process, the preservative sodium chloride is not used. Add zero, or only a very small amount. the

The beneficial effect of the above-mentioned female mosquito olfactory disruptor composition is illustrated through experiments below. the

Test 1: The test method refers to GB13917.8-1992 to detect the mosquito density in the field, and uses the human lure method to investigate the mosquito density. Arrange two fixed investigators in each experimental room (fixed person, fixed point, and timed time). The experimenters rolled up two trouser legs to the knees to expose the skin, illuminated with a flashlight, and used an electric mosquito aspirator to capture mosquitoes that landed on naked mosquitoes for 45 minutes. Mosquitoes on the legs, followed by identification and recording of the number, sex and species of mosquitoes. Mosquito density reduction rate (%)=1-mosquito density in experimental room/mosquito density in blank control room. The test results are shown in Table 1. the

Table 1 Mosquito density and mosquito density reduction rate after using the products prepared in Examples 1-7

As can be seen from Table 1, the mosquito density significantly decreased after using the products prepared in Examples 1-7, and the rate of decline in mosquito density all reached more than 80%. The reduction rate of mosquito density is more than 80%, reaching the effect of traditional mosquito-repellent incense, but the effect of embodiment 1 and embodiment 7 is obviously better than that of traditional mosquito-repellent incense. the

Test 2: The test method is based on GB13917.8-1992, using laboratory and field strains of Culex pisciatus for the test and on-site human-induced bite test, referring to GB13917.4-92, using field strains of Culex pisciatus collected on-site 2-3 days after eclosion was reared in the laboratory, the measurement was carried out, and the number of mosquito bites and the protective effect of the products obtained in Examples 1-7 were observed by the on-site human lure method. Mosquito density reduction rate (%)=1-mosquito density in experimental room/mosquito density in blank control room. the

The on-the-spot prevention mosquito bite result of the product that table 2 embodiment 1-7 makes

As can be seen from the results in Table 2, the number of mosquito bites is greatly reduced after using the products prepared in Examples 1-7, and the protective effects all reach more than 80%, and the protective effects of Examples 1 and 7 reach more than 90%, especially the The protective effect of 1 reaches 100%, the effect of embodiment 1 and embodiment 7 is obviously better than traditional mosquito-repellent incense, and the protective effect of embodiment 2-6 is also slightly better than traditional mosquito-repellent incense. the

Test 3: The test subjects were 20 people, each of whom was bitten by mosquitoes 5 times on average, a total of 100 person-times. After the mosquito bites, immediately use the product spray prepared in Examples 1-7 to smear the 5cm diameter range of the affected part, and start timing, respectively Count the number of people who relieve itching in 1 minute and 3 minutes and the number of people who reduce swelling after 1, 5, 10 and 30 minutes. Calculation method: antipruritic percentage = people who relieve itching / total number of statistics × 100; percentage of swelling = people who reduce swelling / total statistics Person-time × 100. the

Antipruritic detumescence (spray smear) after the product mosquito bite that table 3 embodiment 1-7 makes

deal with 1 minute antipruritic percentage% 3 minutes to relieve itching % 1 minute swelling percentage % % of swelling in 5 minutes 10 minutes to reduce swelling %% 30 minutes swelling reduction percentage Example 1 82 93 52 63 71 85 Example 2 76 87 45 60 65 74 Example 3 74 84 43 55 68 76 Example 4 70 80 41 61 69 75 Example 5 65 76 38 55 62 69 Example 6 64 77 41 55 60 71 Example 6 66 77 43 59 64 76 Example 7 78 90 48 61 69 81

As can be seen from the results in Table 3, the antipruritic effect reached 64-82% after 1 minute of spray application of the product prepared in Examples 1-7, and the antipruritic effect reached 77-93% in 3 minutes, the effect of Example 1 and Example 7 optimal. For the effect of detumescence, the detumescence effect of spraying for 1 minute is 41-52%, the detumescence effect of 5 minutes is 55-63%, the detumescence effect of 10 minutes is 60-71%, and the detumescence effect of 30 minutes is 71-85%. The products prepared in Examples 1-7 have obvious detumescence effects, and the detumescence effects continue to improve as the use time continues, and the detumescence effects of Examples 1 and 7 are the best. the

Test 4: See Table 4 for the effect of the products prepared in Examples 1-7 on preventing and controlling viruses and germs. the

The effect table of the product preventing and treating virus and germ that table 4 embodiment 1-7 makes

As seen from table 4 result, use the product that embodiment 1-7 makes to viruses such as influenza virus, coronavirus, infectious bronchitis virus and Newcastle disease virus and pathogenic bacteria such as streptococcus, typhoid bacillus, dysentery bacillus and tuberculosis bacillus have certain effect. The effect of killing bacteria is better than the effect of virus, wherein the effect of embodiment 1 and embodiment 7 is the best. the

Claims (6)

1. A method for interfering with the sense of smell of female mosquitoes, characterized in that the following composition is used to interfere with the sense of smell of female mosquitoes, the composition contains 0.1-9.9% of the total weight of organic acid as an effective component, containing a total weight of 88- 99.6% water is used as a solvent, and the organic acid is one or more mixtures of n-pentanoic acid, n-hexanoic acid, n-heptanoic acid, and malic acid; water and organic acids are added to the heating mixer according to the above ratio to prepare the product The temperature is 15-35°C, and the product is obtained by stirring until completely dissolved. 2. A method for interfering with female mosquito sense of smell as claimed in claim 1, characterized in that the content of organic acid is 1.0-8.0%. 3. a kind of method for interfering female mosquito's sense of smell as claimed in claim 1 is characterized in that described solvent is distilled water, and content is 90-98%. 4. a kind of method for interfering female mosquito's sense of smell as claimed in claim 1 is characterized in that described organic acid is a food-grade raw material. 5. a kind of method for interfering female mosquito's sense of smell as claimed in claim 1 is characterized in that also containing the sodium chloride of gross weight 0.1-0.5% or citric acid as preservative and gross weight 0.1-0.5% water-soluble Natural fruit and vegetable spices are used as flavoring agents. Add distilled water and organic acid into a heating mixer according to the stated ratio, stir and dissolve, then add preservatives and flavoring agents in turn, and stir until completely dissolved to obtain the final product. 6. A method for interfering with the sense of smell of female mosquitoes as claimed in claim 1, characterized in that: the temperature of the prepared product is 20-30°C.