CN101507702B - An antiseptic facial cleanser - Google Patents

Abstract

The invention discloses a bactericidal facial cleanser, which belongs to the field of hygiene products. The purpose of the present invention is to provide a kind of safe, adopt the bactericidal facial cleanser product of modern biotechnology product processing. The formula of the present invention mainly contains: surfactant, moisturizing agent, nutrient, emollient, essence, bactericide. The invention not only realizes the removal of dirt on the skin by the facial cleanser, but also has good sterilization and disinfection functions. Utilizing low-cost fermented raw materials and adopting modern biological technology, the process is simple and the cost is low. Make the present invention, become a kind of facial cleanser with strong bactericidal power, safety, cheap, high technical content. The bactericide added in the present invention is processed by modern biotechnology from pure natural fermented raw materials, has antiseptic function and is safe. There is no need to add preservatives to the facial cleanser.

Description

A kind of sterilization cleansing milk

Technical field: the invention belongs to field of hygiene products, relate to special cleansing milk.

Background technology:

Cleansing milk has become a lot of people, particularly indispensable article of everyday use in the youngster daily life.

In actual life, along with improving constantly of people's material and cultural life, U.S.A of natural health becomes the target that people pursue.Various skin care item arise at the historic moment on the market.What people used when washing one's face traditionally is with the soap series products of soap as main component.The pH value meta-alkalescence of this series products, inharmonious with the acid ph value of human body skin, thereby zest is bigger, and also the fatty acid resistance to hard water is poor, can form calcium soap, magnesium soap when taking a shower, and is deposited on the skin, hinders the breathing and the metabolism of skin.In order to remedy the deficiency of soap, cleansing milk arises at the historic moment.

Cleansing milk is exactly to adopt the mode of washing to clean the emulsion form product of human body face.The basis of cleansing milk at present commonly used is water and surfactant.Add additives such as essence, wetting agent, antiseptic, antioxidant with stable prod, give its fragrance.

People's perspiration has formed dirt with scurf of dust in air and human body etc.Assembled a large amount of microorganisms on dirt, some are wherein just arranged may be the source of disease microorganism, and these health to people are brought certain threat.Yet,, can not kill the antibacterial of people's face effectively though the most cleansing milk in market can play good cleaning effect.Facial antibacterial can cause people to form acne, also might cause some diseases simultaneously.In the cleansing milk, generally add antiseptic and prevent the putrid and deteriorated of bath gel at present, these antiseptic have certain danger to human beings'health, simultaneously skin are had certain stimulation.

Summary of the invention:

The purpose of this invention is to provide a kind of highly effective and safe, adopt the sterilization cleansing milk product of modern biotechnology product processing.

Prescription of the present invention mainly contains: surfactant, wetting agent, nutrient, emollient, essence, antibacterial.

Major technique feature of the present invention be added have inexpensive, highly effective and safe, all-natural is through the antibacterial of modern biotechnology processing.The used antibacterial of the present invention is the inventor's an invention achievement.Antibacterial is bacillus subtilis LD-8 (Bacillus subtilis LD-8) fermentation broth extract, and culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center, and CGMCC 2294 is numbered in culture presevation).

Fungus characteristic is as follows:

1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the spore.

2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.

3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, the C.I. 13020. positive.

4. 37 ℃ of the suitableeest cultivation temperature, optimum pH 7.0.

This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).

The preparation method of fermentation broth extract is as follows among the present invention:

The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization.

The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours.

Inoculum concentration is 6%～10% o'clock, helps the formation of antibacterial substance.

The fermentation medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, and MgSO4.7H2O 0.7%, and KH2PO4 0.7%, pH8.0.

The fermentation culture conditions of optimizing is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.

Extracting the antibacterial substance method from fermentation liquid is organic solvent precipitation method.Available organic solvent is acetone, methanol, ethanol.

At first with fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant.

With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃.Collect organic facies, adopt vacuum distillation method to remove organic solvent.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.This promptly is the crude extract of our Fungicidal substance that obtains.

The crude extract of Fungicidal substance, behind water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill escherichia coli effectively, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.

This bactericidal liquid has high safety, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse with this bactericidal liquid, and the mouse of experimental group none occur dead, and bacillus subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly bacillus subtilis.Substantially can conclude that this bactericidal liquid is safe for user, can relievedly use.After adding it to cleansing milk, find that it still has good bactericidal activity.

Method for making of the present invention is simple, with deionized water, surfactant (5～20%), wetting agent (3～6%), nutrient (0.5～3%), emollient (0.5～3%), antibacterial (0.1～1%) and essence, join in the reactor, stir, regulate acid-base value, can obtain cleansing cream with high-efficiency sterilization through the degassing, fill.The adding proportion of each material is the percentage ratio of gross weight.

Concrete technology is as follows: deionized water is added in the reactor, its temperature is heated to 70 ℃, the cocamido propyl betaine that adds the regulation ratio then successively, trialkyl methyl oxidation amine, coconut monoethanolamide, glycerol, water-soluble modified silicone oil stirs, and speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5-7.0, add collagen protein, antibacterial, essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 20～30 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed liquor is carried out packing, can obtain efficient sterilizing and wash milk.

Surfactant, this product preferred surfactants has fatty alcohol-polyoxyethylene ether, Oleum Cocois single ethanol amide, cocamido propyl betaine, one or more in cocamidopropyl propyl amide amine oxide, the trialkyl methyl oxidation amine etc.

Glycerol can prevent that water content reduces in the skin, and can improve the tolerance to cold of product as wetting agent, the variation that can not produce form when product is used.

Nutrient is a collagen protein.

Emollient comprises water-soluble modified silicone oil, cation-modified silicone oil, one or more of amino-modified silicone oil.

Citric acid is an amount of, is used to regulate pH value.

Used essence can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.

Beneficial effect:

The present invention has not only realized the dirt on the cleansing milk removal skin,, and possessed good sterilization and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this cleansing milk people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in the cleansing milk utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, cleansing milk with high content of technology.

That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the cleansing milk.

Specific embodiment:

The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.

Specific embodiment:

The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.

Embodiment 1, preparation 100kg product.

The 50kg deionized water is added in the reactor, its temperature is heated to 70 ℃, add the 5kg cocamido propyl betaine then successively, 2kg trialkyl methyl oxidation amine, 2kg coconut monoethanolamide, 4kg glycerol, add the water-soluble modified silicone oil of 3kg then and stir, speed of agitator is unsuitable too high, can be controlled in 25 rev/mins, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5, collagen protein 1kg adds the 0.2kg antibacterial, 0.001kg essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 25 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed liquor is carried out packing, can obtain efficient sterilizing and wash milk.

Get 10% concentration product of the present invention, adopt conventional microorganism detection bactericidal effect, result of the test shows that product bactericidal effect of the present invention reaches more than 90%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.

Claims (1)

1. a bactericidal facial cleanser is characterized in that it contains a bactericide, and the bactericide is a bacillus subtilis CGMCC2294 fermented liquid extract; the 100kg bactericidal facial cleanser product is prepared by the following method: 50kg deionized water is added in the reaction kettle, its temperature is heated to 70 ℃, then add 5kg cocamidopropyl betaine, 2kg trialkylmethylamine oxide, 2kg coconut oleic acid monoethanolamide, 4kg glycerin, Then add 3kg of water-soluble modified silicone oil and stir evenly, control the stirring speed at 25 rpm, lower the temperature to 40°C, adjust the pH value of the solution to 6.5 with citric acid, add 0.2kg of bactericide to 1kg of collagen, 0.001 kg essence, make up the insufficient part with deionized water and stir evenly, the stirring speed can be controlled at 25 rpm, degas the stirred material liquid, use vacuum degassing, control the negative pressure at 5Mpa, remove the mixture during the stirring and dissolving process The air bubbles formed in the liquid reduce the gas content of the material, and the mixed liquid is divided into packages to obtain a sterilized facial cleanser; The preparation method of the Bacillus subtilis CGMCC2294 fermentation broth extract is as follows: The seed medium of the bacterial strain is a broth medium, in which beef extract 3g, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0～7.2, autoclaved steam sterilization; Seed solution culture conditions: culture temperature is 37°C, shaker speed is 140 rpm, fermentation time is 10-14 hours; The composition of the fermentation medium is: 0.3% sucrose, 1% peptone, 0.5% NaCl, 0.7% MgSO ₄ .7H ₂ O, 0.7% KH ₂ PO ₄ , pH 8.0; The fermentation culture conditions are as follows: the culture temperature is 37°C, the shaker speed is 140 rpm, and the fermentation time is 24 hours; First, put the fermentation broth into a high-speed centrifuge at a temperature of 0-4°C and a speed of 10,000 rpm, centrifuge for ten minutes, remove the precipitate, and keep the supernatant; add an equal volume of methanol to the obtained supernatant, and dissolve the The temperature is rapidly lowered to -5°C, and stored at -5°C for 2 hours; the organic phase is collected, and the organic solvent is removed by vacuum distillation; the distillation temperature is 30-60°C, and the distillation is 20 minutes, the organic solvent is removed, and the precipitated substance is collected.