CN101484158A - Senescence inhibitor - Google Patents

Abstract

The present invention provides effective medicines, quasi-drugs and food and beverages for inhibiting aging, improving mitochondrial function, inhibiting muscle function decline, inhibiting muscle atrophy, preventing bedridden, inhibiting muscle aging or improving motor function. The present invention relates to an anti-aging agent, an agent for improving mitochondrial function, an inhibitor of muscle function decline, an inhibitor of muscle atrophy, a preventive agent for bedridden, which contain catechins as active ingredients, and an agent containing catechins and the above-mentioned amino acids as effective ingredients. Ingredient muscle aging inhibitor and motor function improver.

Description

Senescence inhibitor

Technical field

The present invention relates to senescence inhibitor, mitochondrial function improving agent, flesh function reduction inhibitor, amyotrophy inhibitor, be unable to leave the bed preventive, muscle ageing inhibitor and motor function improving agent.

Background technology

Physiological aging and pathological seaility are roughly divided in aging.The physiological aging is the aging that the increase with the age must take place, and the variation of hair, skin, eyes, skeleton, brain etc. occurs, the decline of motor function and energy metabolism.In recent years, the variation of report mitochondrial DNA being arranged is the decline and the old and feeble energy metabolism decline relevant (with reference to non-patent literature 1) that causes of mitochondrial function with damage.

On the other hand, old and feeble muscular strength that produces and endurance decline make daily viability descend.In addition, the decline of energy metabolism and mitochondrial function causes that energy is taken in and the imbalance of energy expenditure, can become the cause of disease of diseases due to habit disturbances such as fat and diabetes.From the viewpoint of improve the quality of living (QOL), prevent that these diseases due to habit disturbances just are considered as important topic by developed country.

Therefore, in order to improve the quality of living and to prolong life-span, inhibition is that so-called inhibition is old and feeble with the physiological function decline of the increase generation at age, and it is very important especially keeping endurance, inhibition fatigue, raising energy metabolism and raising mitochondrial function.

In addition, generally speaking, the amyotrophy that the myoplasm amount of muscle and muscular strength descend can enumerate muscular atrophy of disuse or skeletal muscle reduces disease (Sarcopenia) etc.If generation amyotrophy can be found the flesh function reduction that produces thereupon.Particularly for the old people, can confirm that amyotrophy and muscular strength that the increase because of the age causes descend, and muscle injury and fracture take place easily.If, will speed up muscular atrophy of disuse and myofunctional decline in order to treat and to recuperate and be under the restriction situations such as rest state and plaster fixation.The old people is absorbed in this vicious cycle easily, under the worst case even can become and be unable to leave the bed, therefore, prolong life-span, the quality of supporting the family in order to improve viability, by motion inhibition muscular atrophy of disuse and myofunctional decline to a certain degree, keep motor capacity, it well is vital keeping the globality of body function.

Though senescence process is subjected to the influence of environmental factors and inherited genetic factors, comprise by improvement making it the environmental factors based on living habit of diet and motion to delay.As the means that prevent motor capacity decline, amyotrophy and flesh function reduction that aging is followed, can enumerate daily adhere to when healthy modest movement or recovering physiotherapy etc., particularly under old people's the situation, can enumerate appropriate Sports Practice or rehabilitation practice etc., but, expect safe and more efficient methods from being difficult to adhere to and worrying that injured aspect considers.

People explore the composition of the aging that can suppress the health variation, suppress as the aging of skin that composition is disclosed to be had: the defying age that contains biological collagen synthesis accelerant and abnormal protein remover is with compositions (referring to Patent Document 1), with the antidotal agent (refer to Patent Document 2) of Folium Dauci Sativae extract as effective ingredient, with mustard extract and Medulla junci extract as must composition anti-aging with compositions (referring to Patent Document 3) etc.

In addition, shorten to suppress as telomere (telomere) that composition is disclosed to be had, the cell ageing inhibitor made from the solvent extractable matter of Ganoderma (referring to Patent Document 4); In addition, have as the brain aging inhibitor is disclosed, cocoa and/or cocoa mass (referring to Patent Document 5), organic germanium compounds (referring to Patent Document 6), L-arginine, Phosphatidylserine, docosahexenoic acid, Folium Ginkgo or Folium Ginkgo extract, and tocopherol (referring to Patent Document 7).In addition, disclosed also have, and causes prevention, the therapeutic composition (referring to Patent Document 8) of the clinical symptoms performance of disease unusually as mitochondrial function with the L-arginine.

In addition, people not only depend on motion and physical therapy, also amyotrophy and the flesh function reduction followed thereof are explored according to threpsology's method, and then to preventing bedfast composition to explore.For example, disclose: prevent aging (referring to Patent Document 9) with cystine and theanine, suppress amyotrophy (referring to Patent Document 10) with fruit polyphenol, suppress myoprotein degraded (referring to Patent Document 11) with lycopene, suppress motor capacity decline (referring to Patent Document 12) with organic acid or its salt as effective ingredient with 2 above carboxyls, tired preventive (referring to Patent Document 13) with astaxanthin and/or the making of its ester, amyotrophy inhibitor and the motor function improving agent made with proanthocyanidin (refer to Patent Document 14, patent documentation 15), alleviate the oxidative stress (referring to Patent Document 16) of flesh etc. with superoxide dismutase.Yet present Research is that muscular strength decline and amyotrophic effect that actual aging is followed are not but discussed.

On the other hand, have report to show, contained catechin has effect (referring to Patent Document 20), the inhibition muscular dystrophy effect physiological benefits such as (with reference to non-patent literatures 2) that suppresses cholesterol effect of increasing (referring to Patent Document 17), the effect (referring to Patent Document 18) that suppresses blood sugar increasing, the hardened effect of prevention of arterial (referring to Patent Document 19), raising endurance in green tea, black tea, the cacao bean etc.

But people do not know the effect of catechin aspect old and feeble and mitochondrial function fully, particularly the effect of the decline aspect of the decline of endurance that causes for the increase along with the age and energy metabolism, fatigue, mitochondrial function.

In addition, there is not catechin in muscular atrophy of disuse and follow in the relevant report of related effect aspect its flesh function reduction yet.

On the other hand, known is leucine, valine, isoleucine are treated pharmaceuticals (referring to Patent Document 21), the lasting muscular strength of explosive force as impaired glucose tolerance retention agent (referring to Patent Document 22), elimination myalgia muscle rigidity and swelling benefits such as (referring to Patent Document 23) with branched-chain amino acid.Wherein, reported that leucine has insulin secretion facilitation (with reference to non-patent literature 3) and myoprotein anabolic effect effects such as (with reference to non-patent literatures 4).Yet, use the effect aspect the heavy and muscular strength decline inhibition of the flesh of following in aging when people do not know catechin and amino acids or both fully.

Patent documentation 1: the spy opens the 2004-115438 communique

Patent documentation 2: the spy opens the 2004-51580 communique

Patent documentation 3: the spy opens the 2006-63038 communique

Patent documentation 4: the spy opens the 2003-12539 communique

Patent documentation 5: the spy opens the 2005-281285 communique

Patent documentation 6: the spy opens flat 11-43432 communique

Patent documentation 7: the spy opens the 2003-261456 communique

Patent documentation 8: the spy opens the 2004-182705 communique

Patent documentation 9: the international 2005-123058 pamphlet that discloses

Patent documentation 10: the spy opens the 2001-89387 communique

Patent documentation 11: the spy opens the 2004-59518 communique

Patent documentation 12: the spy opens flat 10-17585 communique

Patent documentation 13: the spy opens the 2006-16409 communique

Patent documentation 14: the spy opens the 2002-338464 communique

Patent documentation 15: the spy opens the 2005-97273 communique

Patent documentation 16: the spy opens the 2006-62976 communique

Patent documentation 17: the spy opens clear 60-156614 communique

Patent documentation 18: the spy opens flat 4-253918 communique

Patent documentation 19: the spy opens flat 4-352726 communique

Patent documentation 20: the spy opens the 2005-89384 communique

Patent documentation 21: the spy opens the 2006-28194 communique

Patent documentation 22: the spy opens the 2000-26290 communique

Patent documentation 23: the spy opens the 2000-26289 communique

Non-patent literature 1: rock ripple lecture modern medicine basis, vol.12, No.2, pp55-58,1999 (Yan Bo Talk Zuo Now are for Yi Ji Foundation, vol.12, No.2, pp55-58,1999)

Non-patent literature 2:Dorchies OM et al, AJP-Cell Physiol, vol.290, No.2, pp616-25,2006

Non-patent literature 3:Brouwer AE et al, Pancreas, vol.6, No.2, pp221-8,1991

Non-patent literature 4:Crozier SJ et al, J Nutr, vol.135, No.3, pp376-82,2005

Summary of the invention

That is, the present invention relates to following invention.

(1) with the catechin is the senescence inhibitor of effective ingredient.

(2) with the catechin be the mitochondrial function improving agent of effective ingredient.

(3) aging that contains catechin suppresses the diet product.

(4) with the catechin be the flesh function reduction inhibitor of effective ingredient.

(5) with the catechin be the amyotrophy inhibitor of effective ingredient.

(6) with the catechin be the preventive of being unable to leave the bed of effective ingredient.

(7) the flesh function reduction that contains catechin suppress with, amyotrophy suppress with or be unable to leave the bed to prevent with the diet product.

(8) with (a) catechin and the muscle ageing inhibitor of the amino acids of (b) from branched-chain amino acid and taurine, selecting as effective ingredient.

(9) with (a) catechin and the motor function improving agent of the amino acids of (b) from branched-chain amino acid and taurine, selecting as effective ingredient.

(10) with (a) catechin and the be unable to leave the bed preventive of the amino acids of (b) from branched-chain amino acid and taurine, selecting as effective ingredient.

(11) the muscle ageing inhibitor that contains (a) catechin and the amino acids (b) from branched-chain amino acid and taurine, selected with diet product, motor function improving agent with the diet product or be unable to leave the bed to prevent with the diet product.

(12) catechin is as the purposes of senescence inhibitor or mitochondrial function improving agent.

(13) catechin is used to make the purposes of senescence inhibitor or mitochondrial function improving agent.

(14) catechin is as flesh function reduction inhibitor, amyotrophy inhibitor or be unable to leave the bed the purposes of preventive.

(15) catechin is used to the purposes making flesh function reduction inhibitor, amyotrophy inhibitor or be unable to leave the bed preventive.

(16) (a) catechin and the amino acids (b) from branched-chain amino acid and taurine, selected as muscle ageing inhibitor, motor function improving agent or be unable to leave the bed the purposes of preventive.

(17) (a) catechin and the amino acids (b) selected from branched-chain amino acid and taurine are used to the purposes making muscle ageing inhibitor, motor function improving agent or be unable to leave the bed preventive.

(18) with the catechin of taking effective dose be feature the inhibition aging method or improve the method for mitochondrial function.

(19) with the catechin of taking effective dose be the inhibition flesh function reduction of feature method, suppress amyotrophic method or prevent bedfast method.

(20) with (a) catechin of taking effective dose and the amino acids (b) from branched-chain amino acid and taurine, selected be feature the aged method of inhibition muscle, improve the method for motor function or prevent bedfast method.

Description of drawings

Fig. 1 is the musculus soleus quality (mg) of matched group and catechin group and non-processed group during tenotomize is handled.

Fig. 2 is the maximum muscle force (g) of matched group and catechin group and non-processed group during tenotomize is handled.

Fig. 3 is the musculus soleus quality (mg) of matched group and catechin group and non-processed group during afterbody suspention is handled.

Fig. 4 is the maximum muscle force (g) of matched group and catechin group and non-processed group during afterbody suspention is handled

The specific embodiment

The present invention relates to provide inhibition aging, raising mitochondrial function, suppress the flesh function reduction, suppress amyotrophy, prevent to be unable to leave the bed, to suppress pharmaceuticals, medicine part outer article and the diet product that muscle wears out and raising motor function aspect is effective, diet is experienced and safe.

The inventor is through discovering:

(1) catechin has the effect of inhibition along with all function reduction of degradation under old and feeble and endurance declines that produces, fatigue, energy metabolism decline, the mitochondrial function, and is useful as having the diet product that suppress old and feeble and raising mitochondrial function effect or pharmaceuticals etc.;

(2) catechin has the minimizing of the limited myoplasm amount that produces down of inhibition action and the effect that maximum muscle force descends, and is useful as diet product with inhibition amyotrophy and muscular strength decline effect or pharmaceuticals etc.; And,

(3) if use catechin simultaneously; with the amino acids of from branched-chain amino acid and taurine, selecting; then have muscular strength decline and amyotrophic effect that inhibition produces along with aging; and have the effect that improves motor function and prevent bedfast effect, it is useful suppressing the aging effect of muscle, the diet product that improve the motor function effect or pharmaceuticals etc. as performance.

Senescence inhibitor of the present invention, the mitochondrial function improving agent, flesh function reduction inhibitor, the amyotrophy inhibitor, be unable to leave the bed preventive, be experienced and few side effects with diet, catechin safe under the situation of taking for a long time or absorbing is as effective ingredient, suppress old and feeble effect as performance, the endurance that particularly suppresses to produce along with aging descends, tired, energy metabolism descends, the effect of all function reduction of degradation under the mitochondrial function, and performance improves the effect of motor functions such as muscular strength, the effect that suppresses the flesh function reduction, it is useful preventing the diet product of bedfast effect and pharmaceuticals etc.

In addition; of the present invention with (a) catechin and the amino acids (b) from branched-chain amino acid and taurine, selected as muscle ageing inhibitor, the motor function improving agent of effective ingredient or be unable to leave the bed preventive;, suppress along with old and feeble and muscular strength that produce descends and the diet product of amyotrophic effect and pharmaceuticals etc. are useful as effective ingredient with catechin safe under the situation of taking for a long time or absorbing and specific amino acids as performance.

In the present invention, so-called " inhibition is old and feeble " is meant that the physiological that inhibition produces along with aging changes, and this physiological variation comprises degradation under endurance decline, fatigue, energy metabolism decline, the mitochondrial function.Particularly, can enumerate the situation that is suppressed at degradation under appreciable endurance decline among the above person in middle and old age of for example one's late 30s that the age increases, fatigue, energy metabolism decline, the mitochondrial function.

In addition, so-called " muscle is aging " is meant the muscle weakness that produces along with aging, for example decline and the amyotrophy of flesh function (muscular strength, muscular endurance, flesh explosive force etc.).So-called " raising motor function " is meant that myofunctional decline keeps or the raising motor capacity by suppressing.

In addition, so-called " amyotrophy " be meant that the myocyte reduces and dwindles the flesh that causes and heavily descend, and can enumerate the plaster fixation that causes in long-term quiet bed and fracture etc. or be exposed to the amyotrophy that causes under the microgravity environment (to be called muscular atrophy of disuse.), and (be called skeletal muscle and reduce disease with the amyotrophy that the increase at age produces.)。Therefore " inhibition amyotrophy " is meant that the flesh that the inhibition inertia or the increase at age are followed heavily descends.

In addition, so-called " flesh function reduction " is the variation of expression muscle recurring structure or matter aspect, the situation that muscular strength, muscular endurance, flesh explosive force etc. descend.Therefore so-called " suppressing the flesh function reduction " is meant the situation of the decline that suppresses muscular strength, muscular endurance, flesh explosive force etc.

In addition, so-called " being unable to leave the bed " is meant, particularly muscular atrophy of disuse and the skeletal muscle that for example can see in the middle-aged and elderly people more than one's late 30s the old people reduces the symptomes complice of disease, for example, become quite few in the treatment of old people's apoplexy and fracture etc. with in recuperating etc. because of quantity of motion, and then the flesh function reduction, situation about causing by bedfast state.

Catechin among the present invention is meant (A) non-phenotype catechins such as catechin, catechin and gallate, nutgall catechin and nutgall catechin gallic acid ester; The general name of (B) phenotype catechins such as epicatechin, epigallo catechin, L-Epicatechin gallate and epigallocatechin gallate (EGCG), and preferably contain the wherein catechin more than a kind.In addition, catechin is preferably non-polymeric.

Being used for catechin of the present invention and generally can directly extracting from Folium Camelliae sinensis, perhaps this Folium Camelliae sinensis extract is concentrated or make with extra care and obtain, also can be product, post highly finished product or the synthetic that derives from other raw materials.

This Folium Camelliae sinensis extracts and can carry out as follows: belonged to by Camellia, for example C.sinensis, C.assamica, or in the Folium Camelliae sinensis that obtains through tea-manufacturing technology of the Folium Camelliae sinensis of their cenospecies, add entry or hot water or add and extract auxiliary agent and extract according to different condition.In addition, also can and use so-called extracting method in nonoxidizing atmosphere, that is, and the method for when noble gases such as boiling the degassing or supply nitrogen is removed dissolved oxygen, extracting.

Comprise in this Folium Camelliae sinensis of making: green tea such as (1) simmers tea, inferior tea, YULU, stone roller tea and pot parched tea; (2) general name is known as the semi-fermented tea such as TIEGUANYIN, color kind, Murrayae Folium Et Cacumen, Wuyi cliff tea of oolong tea; (3) be called the fermented tea such as Darjeeling, Wu Wacha, keemun black tea of black tea.

As extracting auxiliary agent, can enumerate organic acid or its organic acid salts such as sodium ascorbate.

Concentrating of this tea extract can be undertaken by the said extracted thing is concentrated, and the refining of this tea extract can be undertaken by using solvent and post to make with extra care.As the form of the concentrate and the refining thing of tea extract, can enumerate various forms such as solid, aqueous solution, pulpous state.

For example, this tea extract (is also referred to as tea catechin.) can according to the spy open clear 59-219384 number, spy open flat 4-20589 number, spy open flat 5-260907 number, spy open flat 5-306279 number etc. in the method for write up be prepared.In addition, also can use commercially available product, as these commercially available products, can enumerate " POLYPHENON " of Mitsui Norin Co., Ltd., " TEAFURAN ", " SUNPHENON ", the DSMNutritionalProducts " TEAVIGO " of Taiyo Kagaku Co., Ltd., " Sunoolong " of Suntory Ltd etc. of her rattan garden Co., Ltd..

Catechin in this tea extract exists with the form of non-polymer, and exists as the form of solid content that is dissolved in the form in the liquid or is adsorbed in or is contained in the outstanding absurd creature of fine-powder of tea.

In addition, the content of the catechin in this extract is 10～100 quality %, is preferably 30～95 quality %, more preferably 40～80 quality %.In addition, the containing ratio of the catechin in the total polyphenols that comprises in this tea extract is just being made back catechin amount more than 10 quality %, preferably more than 20 quality %.

In addition, in order more effectively to suppress the decline of endurance, tired, the decline of energy metabolism, the following degradation of mitochondrial function is with the decline of the old and feeble all functions that take place, and flesh function reduction, amyotrophy or muscle are aging etc., from nutgall catechin, epigallo catechin, catechin and gallate, L-Epicatechin gallate, the containing ratio of selecting in nutgall catechin gallic acid ester and the epigallocatechin gallate (EGCG) independent or material more than 2 kinds is preferably, more than 40% of catechin total amount that comprises in this tea extract, more preferably more than 60%, more preferably more than 80%.

In addition, in Folium Camelliae sinensis, most catechin is that the form with the phenotype catechin exists, and this phenotype catechin can become the non-phenotype catechin of stereoisomer after processing such as heat, acid or alkali.Therefore, if use non-phenotype catechin, to make aqueous solution from green tea, semi-fermented tea class or the extracting solution of fermentation teas or the concentrate of tea extract, and for example carry out 0.1 minute～120 hours heat treated and obtain non-phenotype catechin at 40～140 ℃.In addition, also can use the high tea of non-phenotype catechin content to propose the concentrate of liquid.They can use separately or use simultaneously.

In addition; during the amino acids that uses (a) catechin simultaneously and (b) from branched-chain amino acid and taurine, select, to the muscular strength decline effect that suppresses to produce, suppress amyotrophy effect, raising motor function effect or prevention and be unable to leave the bed effect synergism is arranged with aging.

This branched-chain amino acid is the aminoacid that side chain is structurally arranged, wherein, and preferred leucine, isoleucine and valine.

In the present invention, preferably at least one in valine, leucine, isoleucine, the taurine is used with catechin.

About the isomer of (b) amino acids, can use the L-type, D-type, any isomer of DL-type.Yet, from naturally occurring viewpoint, preferred L-type.

As the preparation method of (b) amino acids, both can adopt commercially available product, also can be according to known method manufacturing.

Shown in the embodiment,, can suppress decline, the decline of averaged oxygen consumption (energy consumption) and the decline of mitochondrial function as described later with the limit traveling time of aging if make quick aging model mice (SAM-P1) take in tea extract.Therefore, catechin can be used as has the old and feeble effect that suppresses, particularly be senescence inhibitor and the use of mitochondrial function improving agent, also can be used for making these senescence inhibitors etc. with degradation effect under endurance declines that inhibition produces with aging, fatigue, energy metabolism decline, the mitochondrial function.Therefore, be that this senescence inhibitor etc. of effective ingredient can be used as pharmaceuticals that are used for the human or animal and the diet product that performance suppresses old and feeble, improves various effects such as mitochondrial function with the catechin.In addition, when using as the diet product, can be applied to all function reduction of degradation under endurance declines that suppresses to produce with aging, fatigue, energy metabolism decline, the mitochondrial function is diet product, functional food, patient's usefulness food and the specific health food of notion.The decline of the endurance that this senescence inhibitor etc. are especially followed aging, tired add up, bed person, old people and hypomotility person that the decline of the decline of energy metabolism and mitochondrial function causes be effective.

In addition, if make the mice under the action of tenotomize and afterbody suspention processing is limited take in tea extract, then can suppress the minimizing of myoplasm amount and the decline of maximum muscle force.Therefore, catechin can be used as flesh function reduction inhibitor and amyotrophy inhibitor with degradation flesh function reduction effect under limited amyotrophy that causes of inhibition action or the muscular strength and uses, and can be used as inhibition is used by the preventive of being unable to leave the bed that muscular atrophy of disuse and skeletal muscle reduce the state of being unable to leave the bed that disease causes, simultaneously, can be used to make these flesh function reduction inhibitor etc.Be that pharmaceuticals and the diet product that the conducts such as this flesh function reduction inhibitor of effective ingredient are used for the human or animal are useful with the catechin, their performances suppress the not enough or minimizing of the quantity of motion that caused by limited lives of action such as bed, plaster fixation or the work that is seated, to the minimizing of the overload of health or the amyotrophy that the increase at age causes or the effect of myofunctional decline.In addition, if use as the diet product, can be applied to suppress amyotrophy, muscular strength function reduction or prevent bedfast physiological function as notion and the diet product of representing this notion for example the patient with food, old people diet product and functional food with special-purposes such as food and specific health foods.

In addition; take in the combination of (a) catechin and the amino acids of (b) from branched-chain amino acid and taurine, selecting if make quick aging model mice (SAM-P1); then, improve motor function and suppress amyotrophic effect more remarkable than independent absorption (a) catechin or (b) situation of above-mentioned amino acids.Therefore, these compositionss not only can be used as to bed person, old people and hypomotility person bring into play suppress muscular strength descend, suppress amyotrophy, prevention be unable to leave the bed effect muscle ageing inhibitor, motor function improving agent or be unable to leave the bed preventive and use, can also be used to tackle muscle to all groups and wear out, can also be used to make this muscle ageing inhibitor, motor function improving agent or be unable to leave the bed preventive.Therefore, this muscle ageing inhibitor, motor function improving agent or be unable to leave the bed the pharmaceuticals that are used for the human or animal, medicine part outer article and diet product that preventive can be used as performance effect separately and use.

As with senescence inhibitor of the present invention, mitochondrial function improving agent, flesh function reduction inhibitor, amyotrophy inhibitor, be unable to leave the bed preventive, muscle ageing inhibitor and the motor function improving agent administering mode when the pharmaceuticals, can enumerate solid agents such as tablet, capsule, granule, powder for example, syrup, elixir, through intestinal or through the oral administration of liquid preparations such as pipe nutrient, and non-oral administrations such as injection, suppository, inhalant, transdermic absorbent, external agent.In these administering modes, preferred oral administration.

In addition; at the muscle ageing inhibitor, the motor function improving agent that contain (a) catechin and the amino acids (b) from branched-chain amino acid and taurine, selected or be unable to leave the bed in the preventive, can add arbitrarily contain usually in pharmaceuticals, medicine part outer article and the diet product subsidize composition except that dipeptide, inorganic salts etc. such as the above-mentioned aminoacid of allowing, vitamins, carnosine must composition.As this amino acids, can enumerate for example arginine, glycine, lysine, alanine, cystine, cysteine, theanine and methionine as required.

In addition, when these various types of agents of modulation, catechin of the present invention can use separately, perhaps catechin and above-mentioned amino acids can be used simultaneously, or the preparations carrier of pharmaceutically permissible excipient, adhesive, swelling agent, disintegrating agent, surfactant, lubricant, dispersant, buffer agent, antiseptic, correctives, spice, coating material, carrier, diluent etc. can be suitably be used in combination.

Can enumerate the carrier of solid powdery as excipient, saccharides such as glucose, lactose, sucrose for example, amino acidses such as glycine, cellulose etc.As lubricant, can enumerate silicon dioxide, Pulvis Talci, magnesium stearate, Polyethylene Glycol etc.; As adhesive, can enumerate starch, gelatin, methylcellulose, polyvinylpyrrolidone etc.; As disintegrating agent, can enumerate starch, agar etc.

In addition, with senescence inhibitor of the present invention, the mitochondrial function improving agent, flesh function reduction inhibitor, the amyotrophy inhibitor, be unable to leave the bed preventive, when muscle ageing inhibitor and motor function improving agent are used as the diet product, can make cheese (natural cheese, processed cheese), ice cream, yoghourt and other milk product, flavoring agent, soup, rice seasoning bag, Japanese cake class (celestial shellfish etc.), western-style cake class (potato chips, pudding, fruit jelly, chewing gum (Gummy Candy), confection, Fruit candy, caramel, chocolate, chewing gum etc.), fire cake class (Castilla, cake, doughnut, cookies, cookie, pretzel etc.), forms such as various beverages.

When making the diet product, can use catechin separately or can use catechin and above-mentioned amino acids simultaneously, and be used the various raw materials that adopt in the full diet product, come processing and manufacturing according to usual way.

When using as beverage, soda pop (soft drink), noncarbonated beverage products (fruit drink, contain sarcocarp concentrated fruit juice, vegetable beverage etc.), refreshment drink, sports drink, near-water beverage, weight-reducing drinks, tea, coffee, cocoa and alcoholic beverage etc. not only can be provided, simultaneously, also can make up, so that beverage widely to be provided with the Other Drinks composition.

For example, present invariable colour during to long-time preserve and be in harmonious proportion the beverage of transparent outward appearance, also can cater to the hobby of consumer and add the thick extraction tea catechin that contains the such insoluble compound of Folium Camelliae sinensis fine-powder, thereby make it to become to hang turbid beverage.

Thereby preferably above-mentioned beverage is filled into and makes container-packed beverage in the container, as container-packed beverage, can illustration for example, the tea-based, packaged beverages of putting down in writing in non-tea-based, packaged beverages of putting down in writing in No. 3742094 communiques of special permission communique and the Te Kai 2002-272373 communique.An example of representing prescription below.

Container-packed beverage is preferred: consider from the aspect of taste, stability and the effect that can take in for a long time, the catechin ((A) non-phenotype catechin and (B) phenotype catechin) that contains the state that is dissolved in the water of non-polymeric in every 500mL beverage amounts to 50～2500mg, preferably contain 200～2500mg, more preferably contain 300～1300mg, further preferably contain 400～1000mg from the taste aspect.

The content of the catechin in the total polyphenols in the beverage is that the catechin amount after just making is more than the 10 weight %, is preferably more than the 20 weight %.

In addition, when amyotrophy inhibitor or flesh function reduction inhibitor are used as container-packed beverage, shown below for cooperating an example of forming.

This container-packed beverage contains the non-phenotype catechin of non-polymer composition (A) and (B) the phenotype catechin of the state that is dissolved in the water of non-polymeric, and in every 500mL container-packed beverage, the amount of these components is expressed as follows:

Composition (A)=total amount 5～2250mg,

Composition (A)+(B)=total amount 50～2500mg,

Mass ratio (A)/(B)=0.1～9.0.

At this, the non-phenotype catechin of (A) that contains in every 500mL beverage and (B) the total amount of phenotype catechin be 50～2500mg, preferably contain 460～2500mg, more preferably contain 500～1300mg, further preferably contain 600～1300mg, most preferably contain 640～800mg from the taste aspect.

In addition, consider, contain composition (A) 5～2250mg in every 500mL beverage, preferably contain 90～2250mg, more preferably contain 140～2250mg, further preferred 140～1880mg from stable aspect.

The content of the catechin in the total polyphenols in the beverage is that the amount of the catechin after just making is more than the 10 quality %, is preferably more than the 20 quality %.

Further, consider that from taste and stable aspect the weight ratio of composition (A) and composition (B) is (A)/(B)=0.1～9.0, preferred 0.25～9.0, more preferably 0.43～5.67, further preferred 0.67～5.67.

In addition, as 30～98 quality % in the catechin class content, when preferred 40～90 quality % are the material that is selected from epigallocatechin gallate (EGCG), nutgall catechin gallic acid ester, epigallo catechin, the nutgall catechin, the mouthfeel of this beverage is better, and the convergence sense that causes aftertaste is little, and is therefore preferred.At this, contain in epigallocatechin gallate (EGCG), nutgall catechin gallic acid ester, epigallo catechin, the nutgall catechin more than one, contain usually whole these.

In addition; when muscle ageing inhibitor, the motor function improving agent of the amino acids that will contain (a) catechin and (b) from branched-chain amino acid and taurine, select or be unable to leave the bed preventive when using as container-packed beverage; can make and contain (a) catechin and (b) total 100～60000mg of above-mentioned amino acids in every 500mL beverage; preferred 500～20000mg; more preferably 500～10000mg, further preferred 1000～6000mg.

About each aminoacid, preferably contain leucine 100mg～20000mg in every 500mL beverage, more preferably contain 500～4000mg.Contain isoleucine 100mg～20000mg, more preferably contain 500～3000mg.Contain valine 100～20000mg, more preferably contain 500～3000mg.Contain taurine 100～10000mg, more preferably contain 500～3000mg.

Consider that from the chemical stability aspect of taste and catechin the pH of beverage is 3～7 in the time of 25 ℃, preferred 4～7, more preferably 5～7.

In the above-mentioned beverage, can also be with catechin or with the mixture of catechin and above-mentioned amino acids, be used alone or in combination any composition that can add on the prescription, as the additive of antioxidant, essence and flavoring agent, various esters, organic acid, organic acid salt, inorganic acids, mineral acid salt, inorganic salts, pigment, emulsifying agent, antiseptic, flavoring agent, sweeting agent, acidic flavoring agent, juice extraction thing class, vegetable extract class, nectar extract, pH regulator agent and stay in grade agent etc.

Container of the present invention is the same with common beverages can use with polyethylene terephthalate as the container molding (being called the PET bottle) of main component, canister, with the container of the common form of metal forming or the compound paper container of plastic foil, bottle etc.Beverage herein is not meant need dilute beverage capable of direct drinking.

In the time of can pasteurization after for example in the container that beverage is filled into as canister, make the said vesse dixie cup under the sterilization conditions of in food hygiene law, stipulating.When container is not suitable for the distillation sterilization, under the situation as PET bottle or paper container, can adopt following method: in advance with above-mentioned equal sterilization conditions under, carry out between high temperature, short time after the sterilization with for example heat-exchangers of the plate type etc., be cooled to uniform temperature, recharge in the container afterwards.Also can under aseptic condition, in the container of filling beverage, cooperate and fill other compositions.And, can also behind pasteurization under the acid condition, under aseptic condition, pH be transferred to neutrality; Or under neutrallty condition, behind the pasteurization, under aseptic condition, pH is recalled to acidity.

Use food as the patient, can enumerate enteric nutrient that the patient to the old people of adequate nutrition supply difficulty and bed uses and dense thick liquid food (food) etc.

The raw material that can in the mixture of independent catechin or catechin and above-mentioned amino acids, cooperate alimentation composition, and make enteric nutrient and dense thick liquid food according to usual method.Particularly, can or be dispersed in the appropriate solvent, afterwards catechin and other various material dissolutions, homogenize with homogenizer etc., recharge in the various containers of retort pouch or plastics etc.,, make the preparation of aqueous, semi-solid or solid, shaped again by the bactericidal assay of generally acknowledging.In addition, can also with above-mentioned the same homogenize with homogenizer etc. after, make pulverous preparation according to methods such as known spray dryinges.

As an example of alimentation composition, can enumerate the alimentation composition that cooperates protein, saccharide, fat, trace element, vitamins, emulsifying agent and essence and flavoring agent etc.As protein source, can enumerate the isostatic lactoprotein that is of high nutritive value of amino acid nutrient, soybean protein, the protein of albumin etc. also can use their oligopeptide of analyte, albumin and the mixture of other amino acid monomers of Semen sojae atricolor hydrolysate etc.

The feeding of this alimentation composition (absorption) amount is, is converted into calorie as nutrient, and 10～50kcal/ body weight kg every day preferably is grown up.

The use level of the catechin that above-mentioned senescence inhibitor, mitochondrial function improving agent, flesh function reduction inhibitor, amyotrophy inhibitor or be unable to leave the bed contains in the preparations such as preventive, use form and difference according to it, if diet product or pet food etc., its use level is preferred 0.01～5 quality % usually, more preferably 0.05～5 quality %, further preferred 0.1～1 quality %.If the pharmaceuticals beyond above-mentioned, when for example being oral liquids such as oral solid formulations such as tablet, granule, capsule and mixture for internal use, syrup etc., common preferred 0.01～95 quality %, more preferably 5～90 quality %, 5～95 quality %, further preferred 10～80 quality %, 10～95 quality %.

When muscle ageing inhibitor, the motor function improving agent of the amino acids that will contain (a) catechin and (b) from branched-chain amino acid and taurine, select or be unable to leave the bed preventive when using as on-beverage; (a) use level of catechin is used form according to it and different; if when food and pet food etc.; common preferred 0.01～5 weight %; more preferably 0.05～5 weight %, further preferred 0.1～1 weight %.If the pharmaceuticals beyond above-mentioned, for example be that the per os of oral solid formulations such as tablet, granule or capsule, mixture for internal use, syrup etc. is during with liquid preparation etc., common preferred 0.01～95 weight %, more preferably 5～90 weight %, further preferred 10～80 weight %.

In addition, the amount of (b) the above-mentioned amino acids that cooperates in this on-beverage preparation is used form according to it and different, if when food and pet food etc., and preferred 0.01～5 weight % usually, more preferably 0.1～5 weight %, further preferred 0.2～2 weight %.If the pharmaceuticals beyond above-mentioned, when for example being oral liquids such as oral solid formulations such as tablet, granule or capsule, mixture for internal use and syrup etc., common preferred 0.01～95 weight %, more preferably 5～90 weight %, further preferred 10～80 weight %.

Senescence inhibitor of the present invention, mitochondrial function improving agent, flesh function reduction inhibitor, amyotrophy inhibitor, be unable to leave the bed the amount of feeding (effectively intake) of preventive, muscle ageing inhibitor or motor function improving agent, according to degree of patient's sex, age, body weight and symptom etc. and different, adult's amount of feeding every day is, in catechin, preferred 100～3000mg/60kg body weight, more preferably 250～2000mg/60kg body weight, further preferred 250～1000mg/60kg body weight.

In addition; at muscle ageing inhibitor, the motor function improving agent of the amino acids that contains (a) catechin and (b) from branched-chain amino acid and taurine, select or be unable to leave the bed in the preventive; in (b) above-mentioned amino acids; preferred 100～60000mg/60kg the body weight of the amount of feeding every day; more preferably 500～20000mg/60kg, further preferred 1000～6000mg/60kg body weight.These catechins and above-mentioned amino acids in the scope of effect that can obtain multiplying each other, can simultaneously or feed respectively.

Below, in order to illustrate in greater detail the present invention, enumerate the relevant test example that the present composition is carried out, the modulation example of enumerating the present composition then is as embodiment.

Embodiment

Test example 1 (aging of catechin suppresses effect)

In the present embodiment, to use total catechin amount be 81% green tea extract to tea catechin.

In addition, the composition of the catechin in the tea catechin (is for example measured according to the method for generally acknowledging, the spy opens the 2006-129738 communique), it consists of: nutgall catechin (7%), nutgall catechin gallic acid ester (4%), epicatechin (9%), epigallo catechin (23%), L-Epicatechin gallate (12%), epigallocatechin gallate (EGCG) (41%), and other (4%).

(1) respectively organizes the adjustment of mice

After 5 weeks of individual breeding in advance, make it adapt to treadmill exercise the SAM-P1 male mice (quick aging model mice) and the SAM-R1 male mice (generally aging mice) in 13 ages in week, after its domestication, the measuring limit traveling time.

Particularly, mice is undisturbedly stayed on the static treadmill, after treating that it conforms, treadmill is rotated by certain speed, rotate the starting point of the zero hour with treadmill as the beginning of advancing, change belt speed (m/min) at regular intervals, the moment that can't run on treadmill with mice is as the terminal point of advancing and finishing.With this time period as limit traveling time, estimate its endurance.

Belt speed and time during mice limit traveling time is measured are: from the speed of 10m/min, under 10m/min and 15m/min speed, respectively turned round 5 minutes, running is 60 minutes under the 20m/min speed, running is 60 minutes under the 22m/min speed, running is 60 minutes under the 24m/min speed, running is 60 minutes under the 26m/min speed, presses the running of 28m/min speed afterwards.

Divide into groups by SAM-R1 (generally aging mice) matched group, SAM-P1 (quick aging model mice) matched group, SAM-P1 catechin group, every group of 8 mices, and, avoided limit traveling time in the difference between on the same group not.

(2) effect of tea catechin in the quick aging model mice

Press prescription shown in the table 1, modulated control feed of not adding tea catechin and the catechin feedstuff that adds tea catechin.

Feed SAM-R1 matched group and SAM-P1 matched group with control feed,, fed for 10 weeks for every group with catechin forage feed SAM-P1 catechin group.

In addition, each mice group 3 times is weekly pressed 15m/min speed motion 30 minutes at every turn.

Table 1

Feed formula (weight %)

	Control feed	The catechin feedstuff
			Casein	20％	20％
DL-methionine	0.2％	0.2％
			Vegetable oil
	10％	10％
			Potato starch	55.5％	55.15％
Cellulose	8.1％	8.1％
			Mineral
	4％	4％
			Vitamin	2.2％	2.2％
Tea extract (tea catechin)	0	0.35％
			Add up to	100％	100％

After feeding for 8 weeks, measure the limit traveling time of each group.The mice limit traveling time of this moment is as shown in table 2.

Table 2

Feed the mice limit traveling time after 8 weeks

	Limit traveling time (branch) before feeding	Limit traveling time (branch) after 8 weeks
			The SAM-R1 matched group	140.0±3.6	154.8±9.3 *
The SAM-P1 matched group	120.7±7.5	100.0±5.8
			SAM-P1 catechin group	120.1±7.8	121.4±7.1 *

With respect to the significant difference on the statistics of SAM-P1 matched group: ^*P＜0.05

Zmount of oxygen consumption (energy consumption) when in addition, utilizing breast rail to measure each the group peace and quiet of nursing 9 all backs.Use Oxymax system (manufacturing of Columbus company) in the analysis with 8 sensing chamber.Mice is put into sensing chamber make its peace and quiet after 6 hours, measure 24 hours averaged oxygen consumption (the zmount of oxygen consumption mL (mL/kg/min) of the every 1kg mice of every 1min body weight), measured 2 minutes every 18 minutes continuously.24 hours averaged oxygen consumption (mL/kg/min) is as shown in table 3.

Table 3

Feed 24 hourly average zmounts of oxygen consumption (energy consumption) after 9 weeks

	After feeding for 9 weeks
			Zmount of oxygen consumption mL (mL/kg/min)
The SAM-R1 matched group	53.2±1.8 *
		The SAM-P1 matched group	46.9±1.3
SAM-P1 catechin group	51.0±0.9 *

With respect to the significant difference on the statistics of SAM-P1 matched group: ^*P＜0.05

As shown in Table 2, the limit traveling time of the SAM-P1 matched group of the quick aging model mice SAM-R1 matched group of more aging mice significantly shortens, and the aging endurance of following presents decline.

On the other hand, taken in the limit traveling time of SAM-P1 catechin group of catechin feedstuff than SAM-P1 matched group significant prolongation.Hence one can see that: when taking in catechin, suppressed the aging endurance of following and descended indefatigability.

In addition, compare before the limit traveling time of the SAM-P1 matched group of quick aging model mice and the test, reduced about 20 minutes after feeding for 8 weeks.

On the other hand, do not see the minimizing of the limit traveling time of the SAM-P1 catechin group of having taken in the catechin feedstuff.Therefore as can be known, take in catechin and can suppress the aging endurance decline of following.

As shown in Table 3, the zmount of oxygen consumption (energy consumption) of the SAM-P1 matched group of the quick aging model mice SAM-R1 matched group of more aging mice significantly reduces, and this means with the minimizing of aged zmount of oxygen consumption and the decline of energy metabolism.

On the other hand, the zmount of oxygen consumption of having taken in the mice of catechin feedstuff enlarges markedly than the SAM-P1 matched group.Hence one can see that, and catechin descends to the aging energy metabolism of following the inhibition effect.

In addition, as mentioned above, take in tea catechin and traveling time is prolonged or the zmount of oxygen consumption increase, this means that catechin can resisting fatigue.

Test example 2 (effect that the inhibition mitochondrial function of catechin descends)

(1) respectively organizes the adjustment of mice

After 5 weeks of individual breeding in advance, make it adapt to treadmill exercise the SAM-P1 male mice (quick aging model mice) and the SAM-R1 male mice (generally aging mice) in 13 ages in week, after its domestication, the measuring limit traveling time.

Particularly, allow mice undisturbedly stay on the static treadmill, after treating that it conforms, treadmill is rotated by certain speed, rotate the starting point of the zero hour with treadmill as the beginning of advancing, change belt speed (m/min) at regular intervals, the moment that can't run on treadmill with mice is as the terminal point of advancing and finishing.With this time period as limit traveling time, estimate its endurance.

Belt speed and time during mice limit traveling time is measured are: from the speed of 10m/min, under 10m/min and 15m/min speed, respectively turned round 5 minutes, running is 60 minutes under the 20m/min speed, running is 60 minutes under the 22m/min speed, running is 60 minutes under the 24m/min speed, running is 60 minutes under the 26m/min speed, presses the running of 28m/min speed afterwards.

Divide into groups by SAM-R1 (generally aging mice) matched group, SAM-P1 (quick aging model mice) matched group, SAM-P1 catechin group, SAM-P1 exercise group, SAM-P1 catechin/motion combination group, every group of 8 mices, and, avoided limit traveling time in the difference between on the same group not.

(2) effect of tea catechin in the quick aging model mice

Press prescription shown in the table 4, modulation is not added the control feed of tea catechin and is added the catechin feedstuff of tea catechin.

Feed SAM-R1 matched group, SAM-P1 matched group and SAM-P1 exercise group with control feed,, fed for 10 weeks for every group with catechin forage feed SAM-P1 catechin group, SAM-P1 catechin/motion combination group.

In addition, each mice group 3 times is weekly pressed 15m/min speed motion 30 minutes at every turn.

Table 4

Feed formula (weight %)

	Control feed	The catechin feedstuff
			Casein	20％	20％
DL-methionine	0.2％	0.2％
			Vegetable oil
	10％	10％
			Potato starch	55.5％	55.15％
Cellulose	8.1％	8.1％
			Mineral
	4％	4％
			Vitamin	2.2％	2.2％
Tea extract (tea catechin)	0	0.35％
			Add up to	100％	100％

After feeding for 10 weeks, take the gastrocnemius of each group, use ISOGEN (Nippon Gene manufacturing), obtain the RNA sample.Each RNA sample is quantitative, be 5 μ g by per 1 reaction RNA amount, use SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen manufacturing), carry out reverse transcription reaction, obtain cDNA.Reaction condition was made as 42 ℃, 50 minutes 70 ℃, 15 minutes.

The cDNA that obtains if unknown sample with regard to the time with 40 times of its dilutions, and, carry out quantitative PCR by ABI PRISM 7700 Sequence Detector (Applied Biosystems manufacturing) if standard is just diluted 10 times with it with sample.Expression with 36B4mRNA is revised as benchmark, represents with the relative expression quantity of mRNA.

As primer, COX1 GenBank:X57780, nt876-897, nt957-976, COX2 AF37780, nt488-507, nt567-588, COX3 NC_005089, nt9196-9217, nt9308-9327, COX4 NM_053091, nt276-295, nt396-415, heat hock protein 72 (HSP72) has used NM_010479, nt1815-1835, nt1916-1937, PGC-1 α NM_008904, nt642-661, nt751-770, PGC-1 β NM_133249, nt2865-2885, nt2945-2965.

The result is as shown in table 5.

Table 5

Feed gene expression in 10 all backs mice gastrocnemiuss

	The SAM-R1 matched group	The SAM-P1 matched group	SAM-P1 catechin group	The SAM-P1 exercise group	The SAM-P1 combination group
						COX1	127±6 *	100±7	107±7	118±5	117±5
COX2	140±5 *	100±5	102±4	114±3	126±7 *
						COX3	139±6 *	100±7	107±4	118±5 *	125±5 *
COX4	160±7 *	100±7	111±3	126±3 *	141±2 *
						HSP72	147±8 *	100±5	107±4	122±5 *	129±7 *
PGC-1α	146±10 *	100±9	113±6	118±7	124±4 *
						PGC-1β	123±4 *	100±3	104±3	106±6	113±3 *

With respect to the significant difference on the statistics of SAM-P1 matched group: ^*P＜0.05

As shown in Table 5, the SAM-R1 matched group of the more aging mice of the gene expression of COX1～4, HSP72, PGC-1 α, PGC-1 β significantly reduces in the gastrocnemius of the SAM-P1 matched group of quick aging model mice, following the aging expression of gene decline that the gene relevant with electron transport system can take place or be correlated with propagation with mitochondrial differentiation.

On the other hand, share COX2～4 of the SAM-P1 catechin group of catechin feedstuff and motion, HSP72 gene expression and SAM-P1 matched group significantly increase.Hence one can see that, takes in tea catechin and can suppress to follow aging and the decline gene expression relevant with electron transport system or mitochondrial differentiation and propagation that takes place.

In addition, as mentioned above,, mean that mitochondrial function can be kept owing to share the genic inhibition of electron transport system that tea catechin is taken in and motion takes place.

As known from the above, catechin has the aged effect of inhibition, particularly, and to following the old and feeble endurance decline that produces, fatigue, energy metabolism decline, mitochondrial function reduction etc. inhibited, as senescence inhibitor, the mitochondrial function improving agent is useful.

Test example 3 (amyotrophy of catechin suppresses effect, the flesh function reduction suppresses effect)

Using total catechin amount is 81% green tea extract.In addition, the composition of catechin is, nutgall catechin (7%), nutgall catechin gallic acid ester (4%), epicatechin (9%), epigallo catechin (23%), L-Epicatechin gallate (12%), epigallocatechin gallate (EGCG) (41%), and other (4%).

(1) tenotomize (Tenotomy) is handled the effectiveness of catechin in the mice

Male Balb/c mice (9 age in week) was fed for 1 week in advance, be divided into 3 groups (non-processed group, matched group, catechin groups) by body weight, each only organizes n=8.After this, respectively organize 2 weeks of mice with the synthetic forage feed of prescription shown in the table 6.

Table 6

Feed formula (weight %)

	Non-processed group/matched group	The catechin group
			Casein DL-methionine lipid α potato starch cellulose mineral vitamin catechin preparation	20 0.2 10 55.5 8.1 4 2.2	20 0.2 10 55 8.1 4 2.2 0.5
Add up to	100	100

Feed above-mentioned feedstuff after 2 weeks, the hind leg heel string of the mice of matched group and catechin group is implemented tenotomize handle, the tension force that removes the muscle group (musculus soleus etc.) that belongs to hind leg calf position bears a heavy burden.Reduce the following degradation that tensile muscle presents muscular atrophy of disuse, myoplasm amount and muscular strength.Non-processed group is not implemented tenotomize to be handled.

(muscle strength test of excision flesh)

Tenotomize is handled the back and is continued feeding trial 2 weeks of feedstuff, dissects mice.The musculus soleus of excision mice, (ventilation condition: 95%-oxygen, 5%-carbon dioxide) is fixed on the transducer (WPI:FORT100) in 37 ℃ the Krebs solution.Afterwards, (0.2msec is 40Hz) to measure maximum muscle force to implement electricity irritation.The wet quality of musculus soleus as shown in Figure 1, the measurement result of muscular strength is as shown in Figure 2.

(2) effectiveness of catechin in the mice is handled in the afterbody suspention

Male Balb/c mice (9 age in week) was fed for 1 week in advance, be divided into 3 groups (non-processed group, matched group, catechin groups) by body weight, each only organizes n=8.After this, respectively organize 2 weeks of mice with the synthetic forage feed of prescription shown in the table 6.

The feeding trial feedstuff was implemented the afterbody suspention to the mice of matched group and catechin group and is handled after 2 weeks, and the gravity that removes hindlimb muscle group (musculus soleus etc.) bears a heavy burden.Reduce the following degradation that the muscle that bears a heavy burden presents muscular atrophy of disuse, myoplasm amount and muscular strength.Non-processed group is not implemented tenotomize to be handled.

(muscle strength test of excision flesh)

The afterbody suspention is handled the back and was continued the feeding trial feedstuff 10, dissects mice.The musculus soleus of excision mice, (ventilation condition: 95%-oxygen, 5%-carbon dioxide) is fixed on the transducer (WPI:FORT100) in 37 ℃ the Krebs solution.Afterwards, (0.2msec is 40Hz) to measure maximum muscle force to implement electricity irritation.The wet quality of musculus soleus as shown in Figure 3, the measurement result of muscular strength is as shown in Figure 4.

By the result who tests example 3 as can be known, take in catechin and can suppress amyotrophy and muscular strength decline.In addition, in this test example 3, do not confirm by the raising that feeds the heavy and muscular strength of flesh that catechin causes before handling.Hence one can see that, and catechin has and suppresses flesh activity the descend amyotrophy followed and the effect of flesh function reduction, is useful as being unable to leave the bed inhibitor or flesh function reduction inhibitor.

Test example 4 (a) catechin and (b) the aging effect that suppresses of muscle of the mixture of amino acids

As (a) catechin, use the green tea extract of total catechin amount 81%.In addition, the composition of catechin is, nutgall catechin (7%), nutgall catechin gallic acid ester (4%), epicatechin (9%), epigallo catechin (23%), L-Epicatechin gallate (12%), epigallocatechin gallate (EGCG) (41%), and other (4%).In addition, use leucine as (b) amino acids.

With 10 week the SAM-P1 male mice (quick aging model mice) in ages and SAM-R1 male mice (mice generally wears out) after 5 weeks of individual breeding in advance, to avoid the mode of the body weight difference between each group, divide into groups every group of 8 mices by SAM-R1 matched group, SAM-P1 matched group, SAM-P1 catechin group, SAM-P1 leucine group, SAM-P1 catechin/leucine combination group.Use according to synthetic 9 weeks of forage feed of prescription shown in the table 7.In addition, use running water swimming pool, allow each mice by 4 days weekly, every day 5 times, at every turn with 10m/min speed motion 3 minutes.

Table 7

Feed formula (weight %)

	Matched group	The catechin group	The leucine group	Combination group
					Casein
	20％	20％	19.65％	19.65％
					DL-methionine	0.2％	0.2％	0.2％	0.2％
Lipid
		10％	10％	10％	10％
Potato starch						55.5％	55.15％	55.5％	55.15％
	Cellulose	8.1％	8.1％	8.1％	8.1％
Mineral
		4％	4％	4％	4％
Vitamin						2.2％	2.2％	2.2％	2.2％
	Catechin
		0％	0.35％	0％	0.35％
	Leucine
		0％	0％	0.35％	0.35％
	Add up to					100％	100％	100％	100％

Feed 9 weeks back dissection mice.Independent isolating musculus soleus is put into 37 ℃ Krebs solution (ventilation condition: 95%-oxygen, 5%-carbon dioxide), be fixed on transducer (FORT100:World Precision Instruments, Inc.) on.Afterwards, (0.5m second is 140Hz) to measure maximum muscle force to implement electricity irritation.The measurement result of muscular strength is as shown in table 8.

Table 8

The maximum muscle force of musculus soleus when feeding 9 weeks back electricity irritation

With respect to the significant difference on the statistics of SAM-P1 matched group (Dunnet check)

As shown in Table 8, the maximum muscle force of the musculus soleus of the SAM-P1 matched group of the quick aging model mice SAM-R1 matched group of more aging mice significantly reduces, and follows aging maximum muscle force to descend as can be known.In addition, the maximum muscle force of having taken in the mice of cooperation catechin or leucic feedstuff does not confirm significant difference than the SAM-P1 matched group, still, when share catechin and leucine the aging muscular strength decline that causes is had remarkable inhibition effect.

By the above as can be known, catechin among the present invention and amino acids to share as the preparation that is used to suppress follow old and feeble muscular strength to descend be useful.In addition, with the SAM-P1 matched group relatively, the muscular strength of catechin/leucine combination group is improved, hence one can see that, it is effective that catechin/leucic is share improving motor function.

Test example 5 (a) catechin and (b) amyotrophy of the mixture of amino acids suppress effect

As catechin, use the green tea extract of total catechin amount 81%.In addition, the composition of catechin is, nutgall catechin (7%), nutgall catechin gallic acid ester (4%), epicatechin (9%), epigallo catechin (23%), L-Epicatechin gallate (12%), epigallocatechin gallate (EGCG) (41%), and other (4%).In addition, use leucine as amino acids.

With 13 week the SAM-P1 male mice (quick aging model mice) in ages and SAM-R1 male mice (mice generally wears out) after 5 weeks of individual breeding in advance, to avoid the mode of the body weight difference between each group, divide into groups every group of 8 mices by SAM-R1 matched group, SAM-P1 matched group, SAM-P1 catechin/leucine combination group.Use according to synthetic 9 weeks of forage feed of prescription shown in the table 9.In addition, use running water swimming pool, allow each mice by 4 days weekly, every day 5 times, each 10m/min speed motion 3 minutes.

Table 9

Feed formula (weight %)

	Matched group	Catechin/leucine combination group
			Casein
	20％	19.65％
			DL-methionine	0.2％	0.2％
Lipid
		10％	10％
Potato starch				55.5％	55.15％
	Cellulose	8.1％	8.1％
Mineral
		4％	4％
Vitamin				2.2％	2.2％
	Catechin
		0％	0.35％
	Leucine
		0％	0.35％
	Add up to			100％	100％

After feeding for 9 weeks, dissect mice.After diseased abdominal muscle, musculus soleus, plantar muscles, extensor digitorum longus, the quadriceps femoris of mice cut, weigh, obtain total muscle weight.The body weight percentage ratio of total muscle weight is as shown in table 10.

Table 10

Feed the body weight percentage ratio (%) of 9 all backs muscle weights

With respect to the significant difference on the statistics of SAM-P1 matched group (Dunnet check)

As shown in Table 10, the SAM-R1 matched group of the more aging mice of shared muscle weight significantly reduces in the body weight of the SAM-P1 matched group of quick aging model mice, follows aging muscle generation atrophy as can be known.In addition, the muscle weight of having taken in the mice that cooperates catechin and leucic feedstuff has significant difference than the SAM-P1 matched group.

As known from the above, the catechin/amino acids among the present invention to share as being used to suppress to follow old and feeble amyotrophic preparation be useful.

Shown below is the formulation example of senescence inhibitor of the present invention etc.

Formulation example 1 (old and feeble inhibition container-packed beverage)

By the old and feeble inhibition container-packed beverage of the modulation of prescription and condition shown in the table 11.In addition, the composition of the tea extract in this container-packed beverage (tea catechin) is as shown in table 12.Storage stability of this beverage and local flavor are all good.

Table 11

The composition of teas beverage (g/100mL)

	Green tea beverage	Oolong tea beverage
			Green tea extractive liquor (g) oolong tea extracting solution (g) tea extract (g) Purified Water (g) sodium ascorbate (g)	17.5 0 0.37 balances 0.05	08 0.48 balances 0.05
PH (sterilization back)	5.98	5.87
			Sterilization temperature (℃)	133	134
Sterilize heat time heating time (second)	30	30
			Container	Pet container	Pet container

Table 12

Catechin in the beverage is formed (mg/500mL)

	Green tea beverage	Oolong tea beverage
			Catechin	32.4	30.7
Epicatechin	32.7	35.6
			Nutgall catechin	124.1	113.0
Epigallo catechin	85.8	91.8
			Catechin and gallate	23.0	22.1
L-Epicatechin gallate	37.3	41.9
			Nutgall catechin gallic acid ester	94.9	86.7
Epigallocatechin gallate (EGCG)	110.9	120.1
			Non-polymer catechin total amount	541.0	541.9

2 old and feeble inhibition of formulation example with enteric nutrient (dense thick liquid food)

With prescription shown in the table 13, be sealed in the retorts by well-established law after, implement sterilization with high steam, thereby modulated product of the present invention.To meet the amount of national nutritional requirement, minerals uses the organic or inorganic salt mixture of Na, K, Ca, Mg, P, Cl, Fe etc., and vitamins uses vitamin A, D, E, vitamin B ₁, B ₂, B ₆, B ₁₂, C, nicotinic acid, pantothenic acid etc. mixture.

Table 13

Enteric nutrient is formed (g/100mL)

Milk casein	3.40
		Soybean protein isolates	1.67
Dextrin	14.86
		Sucrose	1.30
Soybean oil	1.75
		Perilla oil	0.18
Soybean phospholipid	0.14
		Fatty acid glyceride	0.07
Minerals	0.60
		Vitamins	0.06
Tea catechin (TEAFURAN90S (Yi Tengyuan))	0.11
		Purified Water	Surplus

Formulation example 3 old and feeble inhibition fruit jelly foods

Mixed gel agent 0.65%, 50% concentrated fruit juice 5.0% of grapefruit, citric acid 0.05%, vitamin C 0.05%, the tea catechin (TEAVIGO (DSM NutritionalProducts)) 0.54% of carrageenan and locust bean gum are mixed, add entry and adjust to 100%, 65 ℃ of dissolvings down.Add a spot of grapefruit essence again, keep 5 minutes with after carrying out sterilization processing, be injected in the container of 100mL at 85 ℃.Leave standstill and slowly be cooled to 5 ℃ in 8 hours, obtain containing the fruit jelly food of catechin.

4 old and feeble inhibition of formulation example with vitamin liquid for oral administration

With 800mg taurine, 2000mg sucrose, 50mg caramel, 30mg sodium benzoate, 5mg vitamin B ₁Nitrate, 20mg vitamin B ₂, the 20mg vitamin B ₆, 2000mg vitamin C, 100mg vitamin E, 2000IU vitamin D ₃, 20mg nicotinamide and 100mg tea catechin (Polyphenon 70S (Mitsui agricultural)) join in an amount of Purified Water and dissolve, be adjusted to pH3 with phosphate aqueous solution after, add Purified Water again and make full dose reach 50mL.It was sterilized 30 minutes down at 80 ℃, obtain containing the vitamin liquid for oral administration of catechin.

Formulation example 5 (old and feeble inhibition chewable tablet)

To contain the prescription of 180mg ascorbic acid, 50mg citric acid, 12mg aspartame, 24mg magnesium stearate, 120mg crystalline cellulose, 274mg lactose, 540mg tea catechin (TEAFURAN 90S (Yi Tengyuan)), regulation according to Pharmacopeia of Japan (preparation general provisions " tablet ") is made tablet, obtains containing the chewable tablet of catechin.

Formulation example 6 (old and feeble inhibition tablet)

Modulate the tablet that contains catechin according to well-established law with following prescription.Prescription (weight %): tea catechin (Polyphenon 70S (Mitsui agricultural)) 20%, natrium malicum 20%, palatinose 20%, ascorbic acid 20%, vitamin combination (Japanese spice drug company is made) 5%, crystalline cellulose 5%, sucrose ester 4%, silica 1 %, eggshell calcium 5%.

Formulation example 7 flesh function reduction inhibition/amyotrophy suppress/be unable to leave the bed the prevention (below, be called flesh function reduction inhibition etc.) use beverage

Press prescription and condition shown in the table 14, modulate preparation of the present invention with the form of teas beverage.In addition, the composition of catechin is as shown in Table 15 in this beverage.Storage stability of this beverage and local flavor are all good.

Table 14

The composition of teas beverage (g/100mL)

	Green tea beverage	Oolong tea beverage
			Green tea extractive liquor (g) oolong tea extracting solution (g) tea extract (g) Purified Water (g) sodium ascorbate (g)	17.5 0 0.37 surpluses 0.05	08 0.48 surpluses 0.05
PH (sterilization back)	5.98	5.87
			Sterilization temperature (℃)	133	134
Sterilize heat time heating time (second)	30	30
			Container	Pet container	Pet container

Table 15

Catechin in the beverage is formed (mg/500mL)

	Green tea beverage	Oolong tea beverage
			Catechin epicatechin nutgall catechin epigallo catechin catechin and gallate L-Epicatechin gallate nutgall catechin gallic acid ester epigallocatechin gallate (EGCG) non-polymer catechin total amount	32.4 32.7 124.1 85.8 23.0 37.3 94.9 110.9 541.0	30.7 35.6 113.0 91.8 22.1 41.9 86.7 120.1 541.9

Formulation example 8 (enteric nutrients of usefulness such as flesh function reduction inhibition)

With prescription shown in the table 16, preparation of the present invention is sealed in the sterilization container as enteric nutrient, sterilize by the well-established law autoclaving, and modulate.To meet the amount of national nutritional requirement, minerals uses the organic or inorganic salt mixture of Na, K, Ca, Mg, P, Cl, Fe etc., and vitamins uses vitamin A, D, E, vitamin B ₁, B ₂, B ₆, B ₁₂, C, nicotinic acid, pantothenic acid etc. mixture.

Table 16

The composition of enteric nutrient

Formulation example 9 (fruit jelly food such as flesh function reduction inhibition)

Preparation of the present invention is modulated into fruit jelly food.Mixed gel agent 0.65%, the concentrated fruit juice 5.0% of grapefruit 50%, citric acid 0.05%, vitamin C 0.05%, the tea catechin (TEAVIGO (DSM NutritionalProducts)) 0.54% of carrageenan and locust bean gum are mixed, and add entry and adjust to 100%, dissolve at 65 ℃.Add a spot of grapefruit essence again, and keep 5 minutes to carry out sterilization processing, be injected into afterwards in the container of 100mL at 85 ℃.Leave standstill and slowly be cooled to 5 ℃ in 8 hours, make gelation, obtain orally-dissolvable property good, have a fruit jelly food that fruit-like flavour, mouthfeel are good, contain catechin.

Formulation example 10 (flesh function reduction inhibition etc. are taken liquid orally with vitamin)

Preparation of the present invention is modulated into vitamin liquid for oral administration.With 800mg taurine, 11000mg sucrose, 50mg caramel, 30mg sodium benzoate, 5mg vitamin B ₁Nitrate, 20mg vitamin B ₂, the 20mg vitamin B ₆, 2000mg vitamin C, 100mg vitamin E, 2000IU vitamin D ₃, 20mg nicotinamide and 540mg Polyphenon 70S (Mitsui agricultural) join in an amount of Purified Water and dissolve, be adjusted to pH3 with phosphate aqueous solution after, add Purified Water again and make full dose reach 50mL.It 80 ℃ of sterilizations 30 minutes, is obtained containing the vitamin liquid for oral administration of catechin.

Formulation example 11 (chewable tablet such as flesh function reduction inhibition)

Preparation of the present invention is modulated into chewable tablet.Prescription (every day, amount was 1200mg) with the TEAFURAN 90S (Yi Tengyuan) of 180mg ascorbic acid, 50mg citric acid, 12mg aspartame, 24mg magnesium stearate, 120mg crystalline cellulose, 274mg lactose, 540mg, regulation according to Pharmacopeia of Japan (preparation general provisions " tablet ") is made tablet, obtains containing the chewable tablet of catechin.

Formulation example 12 (tablet such as flesh function reduction inhibition)

Preparation of the present invention is modulated into tablet with following prescription according to well-established law.Prescription (quality %): Polyphenon 70S (Mitsui agricultural) 20%, natrium malicum 20%, palatinose 20%, ascorbic acid 20%, vitamin combination (Japanese spice drug company is made) 5%, crystalline cellulose 5%, sucrose ester 4%, silica 1 %, eggshell calcium 5%.

Formulation example 13 (the aging inhibition teas beverage of muscle)

Modulate the aging inhibition of muscle of the present invention with cooperating amino acid whose teas beverage by prescription and condition shown in the table 17.Storage stability of this beverage and local flavor are all good.

Table 17

The composition (g/100mL) that cooperates the teas beverage of amino acids

	Green tea beverage	Oolong tea beverage
			Green tea extractive liquor (g) oolong tea extract (g) tea catechin (Polyphenon 70S) is Purified Water (g) sodium ascorbate (g) leucine (g) isoleucine (g) valine (g) taurine (g) (g)	17.5 0 0.12 surpluses 0.05 0.16 0.08 0.08 0.05	08 0.16 surpluses 0.05 0.16 0.08 0.08 1.00
PH (sterilization back)	5.98	5.87
			Sterilization temperature (℃)	133	134
Sterilize heat time heating time (second)	30	30
			Container	Pet container	Pet container

Formulation example 14 (the aging inhibition non-tea beverage of muscle)

Modulate the aging inhibition of muscle of the present invention with cooperating amino acid whose sports drink by prescription and condition shown in the table 18.Storage stability of this beverage and local flavor are all good.

Table 18 (g/100mL)

	Cooperate example 1	Cooperate example 2
			Catechin (Polyphenon 70S) is (g) acid (g) Purified Water (g) NaCl (g) KCl (g) essence (g) vitamin C (g) leucine (g) isoleucine (g) valine (g) taurine (g) of sweetener (Aspartame+glucose) (g)	0.114 1.2 0.24 surpluses 0.04 0.009 0.3 0.03 0.12 0.06 0.06 0	0.114 1.2 0.24 surpluses 0.04 0.009 0.3 0.03 000 0.4
PH (sterilization back)	3.5	3.5
			Container	Pet container	Pet container

Formulation example 15 (the aging inhibition enteric nutrient of muscle)

TEAFURAN 90S (Yi Tengyuan), 0.18g leucine, 0.09g isoleucine, 0.09g valine and Purified Water with 3.4g milk casein, 1.67g soybean protein isolates, 14.86g dextrin, 1.3g sucrose, 1.75g soybean oil, 0.18g perilla oil, 0.14g soybean phospholipid, 0.07g fatty acid glyceride, 0.60g minerals, 0.06g vitamins, 0.11g, press well-established law with high temperature high pressure sterilizing, obtain containing aging inhibition of muscle of catechin and amino acids with enteric nutrient (100mL).To meet the amount of national nutritional requirement, minerals uses the organic or inorganic salt mixture of Na, K, Ca, Mg, P, Cl, Fe etc., and vitamins uses vitamin A, D, E, vitamin B ₁, B ₂, B ₆, B ₁₂, C, nicotinic acid, pantothenic acid etc. mixture.

Formulation example 16 (the aging inhibition fruit jelly food of muscle)

Mixed gel agent 0.65%, the concentrated fruit juice 5.0% of grapefruit 50%, citric acid 0.05%, vitamin C 0.05%, tea catechin (TEAVIGO (DSM NutritionalProducts)) 0.54%, leucine 0.3%, isoleucine 0.15%, valine 0.15%, the taurine 0.5% of carrageenan and locust bean gum are mixed; wherein add entry again and adjust to 100%, dissolve at 65 ℃.Add a spot of grapefruit essence again, keep 5 minutes with after carrying out sterilization processing, be injected into respectively in the container of 100mL at 85 ℃.Leave standstill and slowly be cooled to 5 ℃ in 8 hours, make gelation, obtain orally-dissolvable property good, have that fruit-like flavour, mouthfeel muscle good, that contain catechin and branched-chain amino acid is aging to suppress to use fruit jelly food.

Formulation example 17 (muscle is aging to be suppressed with vitamin liquid for oral administration)

With 800mg taurine, 2000mg sucrose, 50mg caramel, 30mg sodium benzoate, 5mg vitamin B ₁Nitrate, 20mg vitamin B ₂, the 20mg vitamin B ₆, 2000mg vitamin C, 100mg vitamin E, 2000IU vitamin D ₃, 20mg nicotinamide, the Polyphenon 70S (Mitsui agricultural) of 50mg, 200mg leucine, 100mg isoleucine, 100mg valine join in an amount of Purified Water and dissolve, after being adjusted to pH3 with phosphate aqueous solution, adding Purified Water again and make full dose reach 50mL.It 80 ℃ of sterilizations 30 minutes, is obtained containing the aging inhibition beverage of muscle of catechin and amino acids.

Formulation example 18 (the aging inhibition chewable tablet of muscle)

Prescription with the TEAFURAN 90S (Yi Tengyuan) that contains 180mg ascorbic acid, 50mg citric acid, 12mg aspartame, 24mg magnesium stearate, 120mg crystalline cellulose, 274mg lactose, 300mg, 600mg leucine, 300mg isoleucine, 300mg valine, regulation according to Pharmacopeia of Japan (preparation general provisions " tablet ") is made tablet, obtains containing the aging inhibition chewable tablet of muscle of catechin and amino acids.

Formulation example 19 (the aging inhibition tablet of muscle)

Modulate the aging inhibition tablet that contains catechin and amino acids according to well-established law by following prescription.Prescription (weight %): Polyphenon 70S (Mitsui agricultural) 5%, leucine 10%, taurine 5%, natrium malicum 20%, palatinose 20%, ascorbic acid 20%, vitamin combination 5%, crystalline cellulose 5%, sucrose ester 4%, silica 1 %, eggshell calcium 5%.

Claims (25)

1. A senescence inhibitor, characterized in that, Contains catechins as active ingredients. 2. The senescence inhibitor as claimed in claim 1, characterized in that, Suppresses the decline in stamina, fatigue, energy metabolism, or mitochondrial function that accompanies aging. 3. A mitochondrial function improving agent, characterized in that, Contains catechins as active ingredients. 4. A food and beverage for antiaging, characterized in that, Contains catechins. 5. The food and drink according to claim 4, wherein Suppresses the decline in stamina, fatigue, energy metabolism, or mitochondrial function that accompanies aging. 6. A muscle function decline inhibitor, characterized in that, Contains catechins as active ingredients. 7. A muscle atrophy inhibitor, characterized in that, Contains catechins as active ingredients. 8. The inhibitor of claim 7, wherein Muscle atrophy is disuse muscular atrophy or sarcopenia. 9. A preventive agent for bedridden, characterized in that, Contains catechins as active ingredients. 10. A food and drink for suppressing muscle function decline, a food and drink for suppressing muscle atrophy, or a food and drink for preventing bedridden, characterized in that, Contains catechins. 11. A muscle aging inhibitor, characterized in that, Active ingredients include (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine. 12. The muscle aging inhibitor according to claim 11, wherein: Muscle aging is a decrease in muscle strength or muscle atrophy. 13. An agent for improving motor function, characterized in that, Active ingredients include (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine. 14. A preventive agent for bedridden, characterized in that, Active ingredients include (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine. 15. A food and drink for muscle aging inhibitors, a food and drink for improving motor function, or a food and drink for preventing bedridden, characterized in that: Contains (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine. 16. The food and drink according to claim 15, wherein Muscle aging is a decrease in muscle strength or muscle atrophy. 17. Use of catechins as an aging inhibitor or a mitochondrial function improving agent. 18. Use of catechins for producing an aging inhibitor or an agent for improving mitochondrial function. 19. Use of catechins as a sarcopenia inhibitor, muscle wasting inhibitor or bedridden preventive agent. 20. Use of catechins for the manufacture of a sarcopenia inhibitor, an amyotrophy inhibitor, or a preventive agent for bedridden state. 21. Use of (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine as a muscle aging inhibitor, an agent for improving motor function, or an agent for preventing bed-ridden. 22. Use of (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine for producing a muscle aging inhibitor, an agent for improving motor function, or an agent for preventing bedriddenness. 23. A method for inhibiting aging or improving mitochondrial function, characterized in that, Take an effective dose of catechins. 24. A method for inhibiting muscle function decline, a method for inhibiting muscle atrophy, or a method for preventing bedridden, characterized in that, Take an effective dose of catechins. 25. A method for inhibiting muscle aging, a method for improving motor function, or a method for preventing bedridden, characterized in that, Taking effective doses of (a) catechins and (b) amino acids selected from branched-chain amino acids and taurine.

Images