CN101480491B - Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof - Google Patents
Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof Download PDFInfo
- Publication number
- CN101480491B CN101480491B CN2009100425481A CN200910042548A CN101480491B CN 101480491 B CN101480491 B CN 101480491B CN 2009100425481 A CN2009100425481 A CN 2009100425481A CN 200910042548 A CN200910042548 A CN 200910042548A CN 101480491 B CN101480491 B CN 101480491B
- Authority
- CN
- China
- Prior art keywords
- water
- collagen polypeptide
- bone
- preparation
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 46
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 45
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 39
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 38
- 102000008186 Collagen Human genes 0.000 title claims abstract description 36
- 108010035532 Collagen Proteins 0.000 title claims abstract description 36
- 229920001436 collagen Polymers 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000002131 composite material Substances 0.000 title claims description 13
- 230000001105 regulatory effect Effects 0.000 title abstract description 14
- 210000000936 intestine Anatomy 0.000 title description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 150000001875 compounds Chemical class 0.000 claims abstract description 25
- 230000000968 intestinal effect Effects 0.000 claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 20
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 13
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 11
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 11
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 10
- 235000002961 Aloe barbadensis Nutrition 0.000 claims abstract 3
- 244000186892 Aloe vera Species 0.000 claims abstract 3
- 239000007788 liquid Substances 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 18
- 229920001542 oligosaccharide Polymers 0.000 claims description 15
- 150000002482 oligosaccharides Chemical class 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 229940036811 bone meal Drugs 0.000 claims description 8
- 239000002374 bone meal Substances 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000003651 drinking water Substances 0.000 claims description 6
- 235000020188 drinking water Nutrition 0.000 claims description 6
- 238000010411 cooking Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 claims 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims 1
- 150000003271 galactooligosaccharides Chemical class 0.000 claims 1
- 239000002002 slurry Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 238000001784 detoxification Methods 0.000 abstract 1
- 230000036449 good health Effects 0.000 abstract 1
- 241001116389 Aloe Species 0.000 description 10
- 241000186016 Bifidobacterium bifidum Species 0.000 description 8
- 206010010774 Constipation Diseases 0.000 description 7
- 229930182830 galactose Natural products 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 241000186660 Lactobacillus Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 229940039696 lactobacillus Drugs 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000010025 steaming Methods 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 3
- 230000001473 noxious effect Effects 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 208000004429 Bacillary Dysentery Diseases 0.000 description 2
- 206010017915 Gastroenteritis shigella Diseases 0.000 description 2
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 241000282894 Sus scrofa domesticus Species 0.000 description 2
- 229930003270 Vitamin B Natural products 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 229960003180 glutathione Drugs 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000014593 oils and fats Nutrition 0.000 description 2
- 230000008855 peristalsis Effects 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 201000005113 shigellosis Diseases 0.000 description 2
- 238000012859 sterile filling Methods 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 241000606125 Bacteroides Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003579 anti-obesity Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 235000003969 glutathione Nutrition 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000008141 laxative Substances 0.000 description 1
- 230000002475 laxative effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
本发明公开了一种骨胶原多肽复合整肠溶液及其制备方法，本发明的骨胶原多肽复合整肠溶液是由以下质量分数的组分配制而成：3％～28％的功能性低聚糖、5％～15％的聚乙二醇，2％～10％的骨胶原多肽粉，1％～5％的芦荟汁和余量的水。该骨胶原多肽复合整肠溶液的功能全面、口感良好、排毒效果明显且能有效调节肠道菌群环境，具有较好的保健效果。The invention discloses a collagen polypeptide compound intestinal regulating solution and a preparation method thereof. The collagen polypeptide compound intestinal regulating solution of the present invention is prepared from the following components in mass fraction: 3% to 28% functional oligomer Sugar, 5%-15% polyethylene glycol, 2%-10% collagen polypeptide powder, 1%-5% aloe vera juice and the rest water. The bone collagen polypeptide compound intestine-regulating solution has comprehensive functions, good taste, obvious detoxification effect, can effectively regulate the intestinal flora environment, and has good health care effect.
Description
Technical field
The invention belongs to technical field of food biotechnology, be specifically related to a kind of compound whole enteric liquid and preparation method thereof.
Background technology
People take in various foods in daily life, and the residue garbage of digesting and assimilating the back generation by body is trapped in the human body intestinal canal easily, by the effects such as noxious bacteria in the intestinal, generates a large amount of toxic products.It is just big more that these toxic products are trapped in the long more harm to human body of intravital time of people, can cause liver and function of intestinal canal disorder, is one of important cause of canceration, aging and various adult diseases.
At present, the whole intestinal health promoting beverage of composite multifunction is not arranged as yet on the market, the whole enteric liquid function of selling on the open market is comparatively single, only has a certain trophic function or health-care effect.Have the minority medicine to can be used for toxin expelling, but its majority can only let out under the relieving constipation, effect of ejecting poison is not good, can not improve flora environment in the human body intestinal canal, can not effectively promote probiotic bacteria propagation, and most whole intestinal class medicine or preparation have certain toxic and side effects.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, provide a kind of complete function, good mouthfeel, effect of ejecting poison obviously and can effectively regulate the bone collagen polypeptide composite solution for regulating intestines of intestinal microbial population environment, the preparation method of the bone collagen polypeptide composite solution for regulating intestines that a kind of technology is simple, cost is less also is provided.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is a kind of bone collagen polypeptide composite solution for regulating intestines, it is characterized in that described compound whole enteric liquid is formulated by the component of following mass fraction: 3%～28% functional oligose, 5%～15% Polyethylene Glycol, 2%～10% bone collagen polypeptide powder, 1%～5% Aloe juice and the water of surplus.
In the technique scheme, 3%～28% the functional oligose that comprises in the described compound whole enteric liquid is mixed by the soybean oligo saccharide of the oligofructose that accounts for compound whole enteric liquid mass fraction 1%～10%, 1%～10% oligomeric galactose and 1%～8% and forms.
In the technique scheme, the mass percent of polypeptide is 70%～80% in the described bone collagen polypeptide powder.
In the technique scheme, the water of described surplus is ultrafiltration water, deionized water or drinking water.
The present invention also provides a kind of preparation method of above-mentioned bone collagen polypeptide composite solution for regulating intestines, may further comprise the steps:
(1) prepares raw material: prepare required functional oligose, Polyethylene Glycol, Aloe juice and bone collagen polypeptide powder; Other prepares a certain amount of drinking water, perhaps light water is adopted ultrafiltration or ion-exchange process (for example can use DOWEX ion exchange resin) handle ultrafiltration water or deionized water; Described Aloe juice is that its meat obtains behind squeezing, fine straining with the Aloe peeling;
(2) preparation: above-mentioned ready raw material is pressed following mass percent mix homogeneously: functional oligose 3%～28%, Polyethylene Glycol 5%～15%, bone collagen polypeptide powder 2%～10% and Aloe juice 1%～5%, surplus are ultrafiltration water, deionized water or drinking water; 3%～28% the functional oligose that comprises in the described mixed material is preferably mixed by the soybean oligo saccharide of the oligofructose that accounts for mixed material mass fraction 1%～10%, 1%～10% oligomeric galactose and 1%～8% and forms;
(3) filtration sterilization: the mixed solution of above-mentioned preparation is filtered, and instantaneous ultrahigh-temperature sterilization (temperature of sterilization is generally 120～130 ℃, and sterilization time is 2～8 seconds) obtains finished product.
In the above-mentioned preparation method, described bone collagen polypeptide powder specifically is to make by the following method: get the Os Sus domestica bone and clean, first broken back boiling water steaming and decocting drains the bone piece and drying behind the oils and fats of removal cooking liquor top layer; Again the bone piece is carried out coarse pulverization, micronizing is made bone meal, adds water bone meal is mixed with bone mud liquid, high temperature sterilize (15min sterilizes under 85 ℃ of temperature) postcooling (naturally cooling to below 50 ℃) also adds the Protamex compound protease and carries out enzymolysis, enzyme denaturing; Get filtrate vacuum concentration and lyophilization after the filtration, obtain bone collagen polypeptide powder.In the preparation process of bone collagen polypeptide powder, the mass ratio of bone piece and water is 1: 1～1: 2 during the boiling water steaming and decocting, and the temperature during the boiling water steaming and decocting is controlled at 110 ℃～120 ℃, and pressure is controlled at 143kPa～198kPa, and the time is controlled at 30～60min.The addition of described Protamex compound protease is 2～2.5 ‰ of an enzymolysis substrate bone meal quality, and the pH value during described Protamex compound protease enzymolysis is 7.5, and temperature is controlled at 55 ℃, and enzymolysis time is 10～15h.Temperature when the bone piece is carried out drying is controlled at 80～85 ℃, and is dried to water content less than 5%; When the bone piece was carried out coarse crushing, micronizing, described bone piece was ground into the granularity of bone meal less than 160 orders; The mass concentration of described bone mud liquid is 10～15%.
The functional oligose that uses in the technique scheme has been widely used in milk product, lactobacillus beverage, bifidobacterium yoghurt, cereal foods and the health food as a kind of food ingredients.In health food, be that the oral liquid that raw material is made can directly be used for regulating intestinal microbial population, loosening bowel to relieve constipation with the oligosaccharide.In the technical scheme of the present invention, the preferred oligofructose that uses can make bacillus bifidus, lactobacillus propagation, bacillus perfringens is suppressed or does not breed, escherichia coli or enterococcus, bacteroid are not bred or the propagation amplitude is lower than bacillus bifidus or lactobacillus, have the function of certain adjusting gastrointestinal bacterial flora; Oligofructose is generated L-lactic acid at big enteral by bacterial fermentation, can dissolving calcium, mineral such as magnesium, ferrum, promote the absorption of human body to mineral, experiment confirm, oligofructose promotes the absorbance of calcium to reach 70.8%, mineral such as dissolved calcium, magnesium can form chelate with the nutrient ossein peptide that adds, and directly absorb to enter in the human body, take in the time of oligofructose and ossein peptide good facilitation has been played in the absorption of human body mineral.Because oligofructose can not directly be digested and assimilated by human body, so its calorific value is low, can not cause fat, and antiobesity action is also arranged indirectly, oligofructose also is a kind of good sweeting agent concerning diabetics.The compound whole enteric liquid that suitably increases the addition of oligofructose is suitable for crowd more, that whole enteric liquid mouthfeel is had relatively high expectations of noxious bacteria in the intestinal and the diabetics that needs bowel relieving.The oligomeric galactose of selecting for use among the present invention is refining through technologies such as enzymolysis dryings and female emulsive oligosaccharide that obtain, bacillus bifidus and lactobacillus to enteral have proliferation function, also have the effectiveness identical simultaneously, can promote enterogastric peristalsis, improve function of intestinal canal with the Plant fiber; The compound whole enteric liquid that suitably increases oligomeric galactose content is fit to the less crowd of daily exercise amount.The soybean oligo saccharide of selecting for use can only be made full use of by bacillus bifidus, promote the propagation of bacillus bifidus, be difficult to be utilized by other harmful bacterium, often the human consumption soybean oligosaccharide can make intravital bacillus bifidus quantity increase, promote intestinal peristalsis promoting, constipation is eased, and it also has the body of promotion and produces physiological functions such as vitamin B group, prevention and treatment bacillary dysentery; The crowd that the compound whole enteric liquid that suitably increases soybean oligo saccharide content is fit to lack vitamin B group and suffers from bacillary dysentery.More than 3 kinds of oligosaccharide have the effect that makes bacillus bifidus, lactobacillus propagation simultaneously.
The Polyethylene Glycol of selecting for use in the technique scheme is that medical circle is generally acknowledged the relatively laxative of the treatment constipation of safety, can help constipation person to set up the defecation rule.The whole enteric liquid that suitably increases polyethyleneglycol content is fit to the comparatively serious crowd of constipation.
The ossein peptide product can increase the breeding of the bacillus bifidus of human body intestinal canal self, the microecosystem regulation system, and have the effect that nutrients such as certain antioxidation and promotion calcium absorb.The Total antioxidant capacity that experimental results show that ossein peptide of the present invention is 71.92% of a glutathion (GSH).
The eliminating toxin and beautifying the skin nutrient of Aloe for generally acknowledging, obviously enhancing body toxin expelling and cleaning function can also make whole enteric liquid have better mouthfeel simultaneously.
Compared with prior art, the invention has the advantages that and selected above-mentioned various nutrients and bone collagen polypeptide powder (preferred homemade bone collagen polypeptide powder) for use, make a kind of multiple functional compound whole enteric liquid after mixing by a certain percentage.This compound whole enteric liquid can make the better collaborative performance of oligofructose, oligomeric galactose, soybean oligo saccharide, Polyethylene Glycol, ossein peptide, aloetic effect, and can be made into the compound whole enteric liquid of function differenceization by the different proportionings of regulating each component, to be applicable to different crowd.Compound whole enteric liquid of the present invention is good mouthfeel not only, and the noxious substance that is adsorbed in the intestinal is excreted, and also can promote probiotic bacteria propagation in the intestinal, regulates intestinal microbial population, loosening bowel to relieve constipation, has health care preferably.Preparation technology of the present invention is also simple relatively, easy to operate, and cost is lower, can realize the suitability for industrialized production of the compound whole enteric liquid of the present invention.
The specific embodiment
Embodiment 1:
A kind of bone collagen polypeptide composite solution for regulating intestines is formulated by the component of following quality: the drinking water of oligofructose 6kg, oligomeric galactose 6kg, soybean oligo saccharide 4kg, Polyethylene Glycol 10kg, bone collagen polypeptide powder 4kg (wherein the mass percent of polypeptide is 75%), Aloe juice 2kg and 68kg.
Above-mentioned bone collagen polypeptide composite solution for regulating intestines is to be prepared by following method: at first be ready to raw material by above-mentioned quality, ready raw material mix homogeneously is made its dissolving, filter, through 125 ℃ of high temperature sterilizes sterile filling immediately after 5 seconds.
The above-mentioned bone collagen polypeptide powder of using is to prepare by the following method: get Fresh Os Sus domestica and clean the preliminary fragmentation in back, bone piece after the fragmentation is put into boiling container, add water in the container with quality such as bone piece, steaming and decocting 50min under 110 ℃ of temperature, 143kPa pressure, remove the upper strata oils and fats then, drain, chopped obtains the bone piece of 3～4cm, dry 7h under 85 ℃ of temperature, after the drying with the bone piece through the pulverizer coarse pulverization, carry out micronizing through super micron mill again, the bone meal after the micronizing is crossed 160 mesh sieves; Bone meal after sieving is added bone mud liquid that water is mixed with 10% mass concentration packs in the container, 15min sterilizes in 85 ℃ of water-baths, be cooled to again below 40 ℃, regulate pH value to 7.5, the Protamex compound protease (Denmark Novozymes Company produce, the mark vigor is 1.5AU/g (Anson unit/gram)) that adds enzymolysis substrate bone meal quality 2 ‰ then is enzymolysis 10h under 55 ℃, the condition of pH value 7.5 in temperature, enzyme denaturing 15min in 85 ℃ of water-baths filters and collects enzymolysis solution then; Use the rotary evaporation concentrating instrument that the enzymolysis solution of collecting is concentrated into 1/4 of original volume, then concentrated solution is placed-30 ℃ of refrigerators to freeze,, collect bone collagen polypeptide powder and be stored in-24 ℃ of refrigerators again through lyophilization 24h.
The above-mentioned Aloe juice of using is that its meat obtains behind squeezing, fine straining with the Aloe peeling; The equal buyable of remaining raw material.
Embodiment 2:
A kind of bone collagen polypeptide composite solution for regulating intestines is formulated by the component of following quality: the deionized water of oligofructose 8kg, oligomeric galactose 8kg, soybean oligo saccharide 6kg, Polyethylene Glycol 12kg, bone collagen polypeptide powder 6kg (wherein the mass percent of polypeptide is 75%), Aloe juice 4kg and 56kg.
Above-mentioned bone collagen polypeptide composite solution for regulating intestines is to be prepared by following method: at first be ready to raw material by above-mentioned quality, ready raw material mix homogeneously is made its dissolving, filter, through 125 ℃ of high temperature sterilizes sterile filling immediately after 5 seconds.
The acquiring way of various raw materials and preparation method are identical with embodiment 1 in the present embodiment.
More than the product of each embodiment carry out inspection for food hygiene according to the method for stipulating among GB/T 5009.11, GB/T 5009.12, GB/T 5009.17 and the GB 4789.2-15, the result is as follows: (in AS, mg/kg), plumbous 0.35 (in Pb for inorganic arsenic 0.2, mg/kg), hydrargyrum (in Hg, mg/kg)≤0.2, total plate count (cfu/g)≤50, coliform (MPN/100g)≤40, mycete (cfu/g)≤10, yeast (cfu/g)≤10, pathogenic bacterium do not detect.Meet GB 16740-1997 health care (function) food universal standard.
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100425481A CN101480491B (en) | 2009-01-19 | 2009-01-19 | Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100425481A CN101480491B (en) | 2009-01-19 | 2009-01-19 | Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101480491A CN101480491A (en) | 2009-07-15 |
| CN101480491B true CN101480491B (en) | 2011-10-19 |
Family
ID=40877879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100425481A Expired - Fee Related CN101480491B (en) | 2009-01-19 | 2009-01-19 | Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101480491B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912543A (en) * | 2017-11-20 | 2018-04-17 | 广州市澳万生物科技有限公司 | Fat collagen solution of suppression based on CRHD and preparation method thereof |
| CN111066991A (en) * | 2019-12-31 | 2020-04-28 | 扬州江大食生一生食品有限公司 | Preparation method of composite prebiotics solid beverage for improving gastrointestinal tract function |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193483A (en) * | 1997-03-18 | 1998-09-23 | 徐海军 | Multi-element polypeptide nutrient and preparation thereof |
| CN1683008A (en) * | 2005-03-14 | 2005-10-19 | 山东轻工业学院 | A calcium-supplementing collagen polypeptide preparation and preparation method thereof |
| CN101041067A (en) * | 2006-03-24 | 2007-09-26 | 林建周 | Preparation for beauty and for adjusting the immunity |
-
2009
- 2009-01-19 CN CN2009100425481A patent/CN101480491B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193483A (en) * | 1997-03-18 | 1998-09-23 | 徐海军 | Multi-element polypeptide nutrient and preparation thereof |
| CN1683008A (en) * | 2005-03-14 | 2005-10-19 | 山东轻工业学院 | A calcium-supplementing collagen polypeptide preparation and preparation method thereof |
| CN101041067A (en) * | 2006-03-24 | 2007-09-26 | 林建周 | Preparation for beauty and for adjusting the immunity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101480491A (en) | 2009-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102578230B (en) | Health-care yoghourt and preparation method thereof | |
| CN104431684A (en) | Health product for increasing bone density and preparation method of health product | |
| CN106173773A (en) | A kind of ferment probiotic bacteria solid beverage and preparation method thereof | |
| CN104542865B (en) | A kind of feature cake based on sweet potato and preparation method thereof | |
| CN110200151A (en) | A kind of yak feed addictive and preparation method thereof that yak survival rate can be improved | |
| CN104489699A (en) | Composition for increasing bone mineral density and preparation method of composition | |
| CN104524549A (en) | Composition capable of improving joints and increasing bone mineral density and preparation method of composition | |
| CN106579202A (en) | Pineapple and passionflower low-sugar jam containing white tea, and making method of pineapple and passionflower low-sugar jam | |
| CN102987275B (en) | A kind of preparation method of the soy oligosaccharides glycopeptide of high biological value | |
| CN102835453A (en) | Making method of vegetable milk | |
| CN102210426A (en) | A kind of pollen paste with moistening effect and preparation method thereof | |
| CN117085107A (en) | Donkey-hide gelatin small molecular peptide and preparation method thereof | |
| CN103520281B (en) | Biological agent for decreasing blood sugar | |
| KR102126458B1 (en) | Method for Manufacturing Fermented Sea Cucumber Tea | |
| CN114982895A (en) | Fermented bovine bone meal prebiotics effervescent tablet and preparation method thereof | |
| CN101480491B (en) | Bone collagen polypeptide composite solution for regulating intestines and preparation method thereof | |
| CN103976205A (en) | Jelly for improving internal secretion and preparation method of jelly | |
| CN111296755A (en) | A nutrition-enriched oatmeal food containing marine chondroitin sulfate and its preparation method | |
| JP3762364B2 (en) | Health food production method using earthworms and ants | |
| CN104888192B (en) | A kind of sea cucumber amino sugar preparation and its production method for increasing bone density | |
| CN108185251B (en) | A kind of Luo Han ginseng turbid juice beverage and preparation method thereof | |
| CN207136152U (en) | A kind of production system of the compound of walnut oligopeptide and date polysaccharide | |
| CN105077279B (en) | The preparation method of an angler stomach cardia peptide and radix glycyrrhizae powder mixture | |
| CN107912543A (en) | Fat collagen solution of suppression based on CRHD and preparation method thereof | |
| KR102712794B1 (en) | Health supplement food that combines seaweed and agricultural products, and manufacturing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111019 Termination date: 20150119 |
|
| EXPY | Termination of patent right or utility model |