CN101422505B - Foot-and-mouth disease vaccine and immunological stress resistance agent of other livestock and poultry and use thereof - Google Patents

Abstract

The purpose of the present invention is to provide an anti-immune stress agent for foot-and-mouth disease and other livestock and poultry vaccines, the percentage concentration of each component is: 5-10% of licorice extract, 3-5% of isomaltooligosaccharide, and 0.5-1% of vitamin C , Carriers (such as bran meal) etc. 84-92%. The stress agent can also be formulated with functional complete feed or concentrated feed or premixed feed. The beneficial effect of the invention is that it can effectively prevent allergic reaction and rise in body temperature after vaccination, increase appetite, increase growth rate by more than 8%, increase antibody level by more than 30%, and shorten feeding period by 3-5 days. The raw materials used in the invention are simple and suitable for large-scale production.

Description

Foot-and-mouth disease vaccine and other livestock and poultry vaccine anti-immune stress agent and application thereof

technical field

The invention relates to the fields of animal health care, biomedicine and functional feed, and develops an anti-immune stress agent for foot-and-mouth disease and livestock and poultry vaccines, functional feed and applications thereof.

Background technique

Immune stress refers to the occurrence of allergies in individual animals after vaccination, a few body temperature rises, most anorexia, metabolic changes (such as a sharp increase in carbohydrate utilization, accelerated gluconeogenesis and glycogenolysis in the liver; increased nitrogen excretion, peripheral protein Accelerated decomposition, decreased skeletal muscle deposition, increased protein synthesis in the acute phase), slowed growth, and prolonged feeding periods. The immune stress of pigs refers to the phenomenon that some individuals develop allergies after vaccination, a few have elevated body temperature, most of them have food loss, slow growth, and the feeding cycle is prolonged for 3-5 days. In pig production, piglets and finishing pigs need to be inoculated with 5-6 kinds of vaccines, and the number of vaccinations is 8-10 times. Especially before the age of 60 days, the intensity of immune stress is greater, which has the greatest impact on the growth and development of piglets.

Immune stress also affects the popularization and application of vaccines and the prevention and control of major diseases. Foot-and-mouth disease is an acute, febrile, and highly contagious infectious disease of artiodactyls (pigs, cattle, sheep, camels, deer, etc.) caused by foot-and-mouth disease virus. The disease is highly contagious and spreads rapidly, often causing a pandemic, causing great harm to animal husbandry production, especially pig raising, and becoming the primary quarantine disease for animal quarantine in the world. At present, the main measures taken for the prevention of foot-and-mouth disease are immunization injections, but the immune stress response of the foot-and-mouth disease vaccine is particularly severe. Most people are unwilling to vaccinate pigs, cattle, and sheep, which brings certain difficulties to the prevention of foot-and-mouth disease. For example, in 2002, Zhongning County immunized 5 piglets with freeze-dried swine fever vaccine and 2mL foot-and-mouth disease oil vaccine for the entire county, and wore ear tags. Anaphylactic shock reactions often occurred after immunization. Severely affected pigs died on the spot; on October 15, 2002, after the foot-and-mouth disease vaccine was injected in a township of Heihe City, Heilongjiang Province, 13 sheep became ill, and 3 of them were seriously ill, screaming, lying on the ground, and breathing quickly , Visible mucous membrane congestion, muscle tremor, rumen gas, and white foam at the corners of the mouth; the veterinary station of Yangshe Town, Zhangjiagang City, Jiangsu Province, on the afternoon of October 16, 2003, raised more than 180 animals from the 12th group of Qian Renxiang in Chengdong Village of the town. Only goats were immunized against foot-and-mouth disease, and 13 of them died within 5 days after immunization.

The anti-immune stress agent and the corresponding functional feed have the functions of anti-immune stress, increasing animal daily gain, improving feed conversion rate, and improving animal immune function. Therefore, it is of great significance to develop anti-immune stress agents, especially anti-foot-and-mouth disease vaccine stress agents and corresponding functional feeds.

Contents of the invention

The object of the present invention aims to provide a kind of anti-immune stress agent of foot-and-mouth disease and other livestock and poultry vaccines

The second object of the present invention is to provide the anti-immune stress functional feed of foot-and-mouth disease and other livestock and poultry vaccines

The third object of the present invention is to provide the application method of anti-immune stress agent and functional feed.

The purpose of the present invention is achieved in the following manner:

An anti-immune stress agent for foot-and-mouth disease and other livestock and poultry vaccines comprising: licorice extract, isomaltooligosaccharide, vitamin C, carrier; licorice extract accounts for 5-10% of the total mass of the stress agent, and isomaltooligosaccharide 3-5% of the total mass of the stress agent, vitamin C accounts for 0.5-1% of the total mass of the stress agent, and the carrier accounts for 84-92% of the total mass of the stress agent.

The carrier is: bran meal or medical stone or zeolite powder

An anti-immune stress functional feed for foot-and-mouth disease and other livestock and poultry vaccines is an anti-immune stress functional full-price feed, an anti-immune stress functional concentrated feed or an anti-immune stress functional premixed feed.

The described anti-immune stress functional complete feed contains 500-1000 grams of licorice extract, 300-500 grams of isomaltooligosaccharide, and 50-100 grams of vitamin C in every ton of complete feed; Stress functional concentrates or anti-immune stress functional premixes are based on 500-1000 grams of licorice extract, licorice extract, and vitamin C in each ton of concentrated feed or premix feed. 300-500 grams of isomaltooligosaccharide and 50-100 grams of vitamin C are multiplied by their concentration times.

The application method of the anti-immune stress agent of the foot-and-mouth disease and other livestock and poultry vaccines lies in the following steps: 4-8 days before the injection of the foot-and-mouth disease vaccine and the swine fever vaccine, add the present invention in the proportion of 0.5-1.5% to the full-price feed of the original feeding Mix the anti-immune stress agent, feed for 4-8 days, then inject the vaccine, and continue to feed for another 8-15 days.

The application method of the described foot-and-mouth disease and other livestock and poultry vaccine anti-immune stress functional feed lies in the following steps: 4-8 days before the injection of the foot-and-mouth disease vaccine or swine fever vaccine, start feeding the above-mentioned anti-immune stress functional full-price feed 4- 8 days, and then continue to feed for 8-15 days after vaccination.

The licorice extract was purchased from Xi'an Aojing Technology Development Co., Ltd., China.

The premixed feed is composed of trace elements (iron, copper, manganese, zinc, iodine, selenium), vitamins (A, D, E, B1, B2, etc.), amino acids, anti-disease and growth-promoting drugs, and carriers in a certain proportion. . Concentrated feed is also called protein concentrated feed. Its raw materials contain the following substances: minerals, including calcium hydrogen phosphate, stone powder (calcium powder) or shell powder; trace elements (such as copper sulfate, manganese sulfate, etc.); amino acids, antioxidants , antibiotics, protein feed and multivitamins.

According to requirements, the licorice extract, isomaltooligosaccharide, vitamin C and complete feed, concentrated feed or premixed feed are uniformly mixed according to the above ratio to obtain the anti-immune stress functional feed for foot-and-mouth disease and other livestock and poultry vaccines.

The principle of the present invention is that licorice and its extracts have glucocorticoid-like effects, thus have anti-allergic effects, can inhibit the production and release of inflammatory mediators and inhibit the effects of inflammatory mediators to alleviate symptoms. This feature of the extract can eliminate the allergic reaction caused by the vaccine, especially the foot-and-mouth disease vaccine; The best use time is 15 days; Isomaltooligosaccharide can also enhance immunity and increase antibody levels; VC, etc. are also closely related to the production of immune function antibodies, and also have anti-stress effects.

The invention has the beneficial effects of effectively preventing allergic reactions and body temperature rises caused by immune stress of vaccines, increasing appetite, increasing growth speed by more than 8%, increasing antibody levels by more than 20%, and shortening the feeding period by 3-5 days. The invention uses simple raw materials, is convenient to prepare, is non-toxic and has no side effects, is suitable for large-scale production, and can save costs, and is a good method for preventing vaccine immune stress, especially foot-and-mouth disease vaccine immune stress.

Detailed ways

The following embodiments and examples are intended to further illustrate the present invention rather than limit the present invention.

Prepare the anti-immune stress agent for foot-and-mouth disease and livestock and poultry vaccines according to the following percentages: 5-10% of licorice extract, 3-5% of isomaltooligosaccharide, 0.5-1% of vitamin C, and 84-92% of bran meal.

Formulate anti-immune stress functional feed for foot-and-mouth disease and livestock and poultry vaccines according to the following additions: 500-1000 grams of licorice extract, 300-500 grams of isomaltooligosaccharide, and 50-100 grams of vitamin C are contained in each ton of complete feed. Obtain anti-immune stress functional full-price feed; or the content of licorice extract, isomaltose and vitamin C in each ton of concentrated feed or premixed feed is 500-1000 grams of licorice extract, 300-100 grams of isomaltooligosaccharide 500 grams, 50-100 grams of vitamin C are multiplied by their concentration times respectively to obtain the anti-immune stress functional concentrate or premix.

Example 1:

Select 24 piglets with an average body weight of 12.4 kg, and randomly divide them into groups A, B, and C, with 8 piglets in each group. The basic full-price diet is: 66% corn, 8% wheat bran, 22% soybean meal, and 4% premix; add 0.75% of the anti-immune stress agent of the present invention to the aforementioned basic diet and mix evenly to obtain anti-immune stress functional feed. Group A was injected with normal saline, group B was injected with foot-and-mouth disease vaccine, and group C was fed with anti-immune stress functional feed 5 days before vaccination, and then injected with vaccine for 10 days. Body temperature changes were measured 2 hours and 12 hours after vaccination. The body temperature of group A did not change, and the feed intake was normal; the body temperature of group B increased by 1 degree and 0.7 degree respectively, and there was leftover material in the trough on the second day; the temperature of group C increased by 0.5 degree and 0.3 degree respectively, and the food intake speed was slow on the second day , but there is no material left in the tank. Weigh after 10 days, measure feed-to-meat ratio and daily weight gain respectively as follows:

Table 1: Effects of anti-immune stress agents on daily gain and feed-to-meat ratio of piglets

The above results show that the beneficial effect of the present invention is to better solve the rise in body temperature and allergy caused by the foot-and-mouth disease vaccine, significantly improve the daily gain, and reduce the feed-to-meat ratio.

Example 2

The anti-immune stress functional full-price feed of the present invention was fed, and other conditions were the same as in Example 1, and the anti-immune stress effect was good.

Claims (3)

1. a foot-and-mouth disease and other livestock and poultry vaccine anti-immune stress agent, is characterized in that, described anti-immune stress agent is made up of following composition: Radix Glycyrrhizae extract, isomaltooligosaccharide, vitamin C, carrier; Radix Glycyrrhizae extract accounts for 5-10% of the total mass of the anti-immune stress agent, isomaltooligosaccharide accounts for 3-5% of the total mass of the anti-immune stress agent, and vitamin C accounts for 0.5-5% of the total mass of the anti-immune stress agent. 1%, the carrier accounts for 84-92% of the total mass of the anti-immune stress agent. 2. The anti-immune stress agent for foot-and-mouth disease and other livestock and poultry vaccines according to claim 1, characterized in that, the carrier is bran meal or medical stone or zeolite powder. 3. A foot-and-mouth disease and other livestock and poultry vaccine anti-immune stress functional feed, characterized in that, the functional feed is an anti-immune stress functional full-price feed, an anti-immune stress functional concentrated feed or an anti-immune stress functional feed. Stimulant functional premix; said anti-immune stress functional full-price feed contains 500-1000 grams of licorice extract, 300-500 grams of isomaltooligosaccharide, and 50-100 grams of vitamin C in every ton of full-priced feed. grams; the anti-immune stress functional concentrate or anti-immune stress functional premix is that the content of licorice extract, isomaltooligosaccharide and vitamin C in every ton of concentrated feed or premixed feed is based on the content of licorice 500-1000 grams of extract, 300-500 grams of isomaltooligosaccharide, and 50-100 grams of vitamin C are multiplied by their concentration times.