CN101407834A - Method for preparing collagen peptide by using cod skin - Google Patents
Method for preparing collagen peptide by using cod skin Download PDFInfo
- Publication number
- CN101407834A CN101407834A CNA2008101596172A CN200810159617A CN101407834A CN 101407834 A CN101407834 A CN 101407834A CN A2008101596172 A CNA2008101596172 A CN A2008101596172A CN 200810159617 A CN200810159617 A CN 200810159617A CN 101407834 A CN101407834 A CN 101407834A
- Authority
- CN
- China
- Prior art keywords
- collagen
- neutral
- cod
- collagen peptide
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 43
- 108010035532 Collagen Proteins 0.000 title claims abstract description 43
- 229920001436 collagen Polymers 0.000 title claims abstract description 28
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 30
- 230000007935 neutral effect Effects 0.000 claims abstract description 17
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 5
- 238000007598 dipping method Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- 229960000583 acetic acid Drugs 0.000 claims description 6
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 6
- 239000000920 calcium hydroxide Substances 0.000 claims description 6
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 6
- 239000012362 glacial acetic acid Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 108090000145 Bacillolysin Proteins 0.000 claims description 4
- 102000035092 Neutral proteases Human genes 0.000 claims description 4
- 108091005507 Neutral proteases Proteins 0.000 claims description 4
- 230000003252 repetitive effect Effects 0.000 claims description 4
- 238000005201 scrubbing Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 2
- 238000013467 fragmentation Methods 0.000 claims description 2
- 238000006062 fragmentation reaction Methods 0.000 claims description 2
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims 2
- 239000011575 calcium Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000004071 biological effect Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- 239000013505 freshwater Substances 0.000 abstract 3
- 239000000463 material Substances 0.000 abstract 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 14
- 239000000243 solution Substances 0.000 description 11
- 239000012153 distilled water Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 239000011259 mixed solution Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- 241000276446 Gadiformes Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to a method for preparing a collagen peptide by taking a cod skin as a material; in the method, the cod skin is selected as the material and is washed clearly by fresh water; 0.2 percent-0.5 percent mixed liquid of calcium hyduloxide and sodium hydroxide is added for dipping; then the cod skin is washed by fresh water until the pH value is neutral; after the water liquid of 0.3 percent to 0.6 percent diluted acid is added for dipping, the cod skin is washed by fresh water again until the pH value is neutral; the cod skin is cracked and is stirred and extracted in hot water for extracting a collagen; then undissolved substances are removed through filtering; the pH value of the extraction liquid of the collagen is adjusted to be neutral; under the stirring conditions, enzymes are added for carrying out zymohydrolysis and obtaining a zymohydrolysis liquid; and after the enzymes are eliminated and filtering is carried out, the powder of the collagen peptide is obtained through vacuum condensing and sponging drying. The method has the advantages of simple technique, less device investment, no environment pollution and being suitable for industrialization production. The collagen content of the powder of the prepared collagen peptide is higher; and the color, smell and biological properties are excellent. The powder of the collagen peptide can be broadly applied to the industries of health-care foods, medicines, and the like.
Description
Technical field
The present invention relates to a kind ofly prepare the method for the amino acid whose peptide of uncertain number with fish, especially a kind of is the method for feedstock production collagen peptide with the cod skin.
Background technology
Collagen protein (collagen) claim collagen again, be the spirality fiber shape protein that twists into by three peptide chains, be a kind of glycoprotein, molecule contains sugar and a large amount of glycine, proline(Pro), oxyproline etc., exist in all multicellular animals bodies, it is the maximum class protein of in-vivo content, it accounts for 1/4th of total protein content in human body, be present in nearly all tissue, it is a kind of extracellular protein, exist with the insoluble fiber form, to animal and human's body skin, blood vessel, bone, muscles and bones, the formation of cartilage is very important, being the important substance of reticular tissue, is decision reticular tissue flexible principal element, plays the part of the role of conjunctive tissue in zooblast.Collagen protein belongs to bioabsorbable polymer material, and it is used and has related to fields such as medicine, makeup, food.At present, research about collagen protein is more, especially more with the collagen protein research of Mammals such as pig, ox etc., and the research of the collagen protein in the aquatic products is less, and in recent years along with the outburst of mad cow disease and foot and mouth disease, caused that people to the security of products of using these sources and to the concern of the influence of human health situation, therefore extract the important subject that collagen protein just becomes the aquatic products investigator from fishery products.In addition, the nourishing function of biologically active peptides and pharmacological action are the present focuses of research, not only be easy to digest and assimilate by the resulting collagen peptide of directional enzymatic, and have hypotensive, reducing blood-fat, enhancing immunity activity, the anti-ageing nourishing function of waiting for a long time.
Cod is often referred to the Gadiformes fish, is the extended familys of Sea World, known have 500 approximately surplus kind, be that the master of marine fishery catches object.Fast development along with China's ocean industry, cod becomes the important aquatic products processing raw material of China, year amount of finish is about ten thousand tons of 40-50, mainly concentrate on coastlands such as Shandong, in the process of processing, produce and comprise head, the tankage that internal organ and epidermis etc. are a large amount of, wherein fish-skin accounts for the 5-10% of fish body, account for the 7-8% of amount of finish, to the processing of these tankage, most of factories have adopted to bury and have abandoned or as the raw material of producing fish meal, the added value that it produced is low, not only cause great waste, but also can cause the pollution of environment.Contain abundant collagen protein in the fish-skin, content is the highest to be accounted for more than the 70-80% of its dry-matter, more much higher than other position of fish body.Therefore consider from the angle of yield, from fish-skin, extract collagen protein and have more economic implications.
Summary of the invention
Collagen protein with the terrestrial animal preparation has potential safety hazard, and cod skin abandons or as the raw material of production fish meal in order to overcome in the prior art, the added value that is produced is low, and the deficiency of easy contaminate environment, the invention provides a kind of preparation method of cod collagen peptide, the present invention can not only make full use of the tankage waste of aquatic products processing, improve the added value of aquatic products processing, and can reduce the pollution of environment.
The technical solution adopted for the present invention to solve the technical problems is: a kind of preparation method of cod collagen peptide, it is characterized in that: choosing cod skin is raw material, use the clear water wash clean, add calcium hydroxide and Qing Yangization Na mixed liquid dipping 8-14 hour, the mixed liquid concentration of calcium hydroxide and Qing Yangization Na is 0.2-0.5%, wherein the weight ratio of calcium hydroxide and Qing Yangization Na is 1: 2-4, use the clear water repetitive scrubbing then, and make its pH value to neutral; Adding concentration is that the dilute acid solution of 0.3-0.6% soaked 1-3 hour, uses the clear water repetitive scrubbing again, makes its pH value to neutral; With the fish-skin fragmentation, in 70-80 ℃ of hot water, stir, extracting 8-10 hour, extract collagen protein, remove by filter insolubles; It is neutral that the collagen protein extracting solution is regulated pH value, under agitation condition, adds enzyme and carries out enzymolysis 3-5 hour, and hydrolysis temperature is 40-50 ℃, and enzymolysis solution goes out after enzyme, the filtration, through vacuum concentration, and spraying drying, acquisition collagen peptide powder.
The dilute acid solution of described immersion cod skin is a glacial acetic acid solution.
The used enzyme of described collagen protein enzymolysis extracting solution is a kind of in neutral protease, Sumizyme MP or the bromeline.
Abundant raw material of the present invention is easy to get, and collagen content is higher, and technology is simple, and facility investment is few, and non-environmental-pollution is suitable for suitability for industrialized production.The collagen peptide powder that adopts the inventive method to make is easy to digest and assimilate, and color and luster, smell, biological property etc. are all better, can be widely used in industries such as protective foods or medicine.
Embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
Get cod skin 0.5Kg fresh or that thaw, clean 4-5 time, drain with 2L water; Water intaking 1L adds the Ca (OH) of entry weight 0.1%
2Make mixed solution with 0.2% NaOH, cod skin is put in the soak solution soaked 10 hours, remove by filter supernatant liquor, the throw out water cleans repeatedly and makes its pH value to neutral, adding concentration is 0.4% Glacial acetic acid 1L, soaked 2 hours, and removed by filter supernatant liquor, the throw out water cleans the pH value repeatedly to neutral.Distilled water is heated to 75 ℃, under constant temperature stirs, the distilled water extracting of adding 2L 9 hours, remove by filter insolubles, the filtered liquid temperature is cooled to about 48 ℃, with NaOH adjust pH to 7, under constant temperature stirs, the neutral protease that adds 0.3g, enzymolysis 4 hours, 100 ℃ of enzyme 5min that go out are through vacuum concentration, last spraying drying obtains the collagen active peptide powder.
The collagen active peptide powder that is obtained, its color and luster, local flavor and biological property are better, and protein content is up to 90% (with dry weight basis), and the molecular weight of 75% collagen peptide is below 1000Da.
Embodiment 2
Get cod skin 1Kg fresh or that thaw, clean 4-5 time, drain with 4L water; Water intaking 2L adds the Ca (OH) of entry weight 0.05%
2Make mixed solution with 0.15% NaOH, cod skin is put in the soak solution soaked 14 hours, remove by filter supernatant liquor, the throw out water cleans repeatedly and makes its pH value to neutral, adding concentration is 0.3% Glacial acetic acid 2L, soaked 3 hours, and removed by filter supernatant liquor, the throw out water cleans the pH value repeatedly to neutral.Distilled water is heated to 75 ℃, under constant temperature stirs, the distilled water extracting of adding 4L 8 hours, remove by filter insolubles, the filtered liquid temperature is cooled to about 48 ℃, with NaOH adjust pH to 7, under constant temperature stirs, the Sumizyme MP that adds 1.5g, enzymolysis 3 hours, 100 ℃ of enzyme 5min that go out are through vacuum concentration, last spraying drying obtains the collagen active peptide powder.
Embodiment 3
Get cod skin 3Kg fresh or that thaw, clean 4-5 time, drain with 12L water; Water intaking 6L adds the Ca (OH) of entry weight 0.2%
2Make mixed solution with 0.3% NaOH, cod skin is put in the soak solution soaked 8 hours, remove by filter supernatant liquor, the throw out water cleans repeatedly and makes its pH value to neutral, the Glacial acetic acid 6L that adds concentration 0.6%, soaked 1 hour, and removed by filter supernatant liquor, the throw out water cleans the pH value repeatedly to neutral.Distilled water is heated to 75 ℃, under constant temperature stirs, the distilled water extracting of adding 12L 10 hours, remove by filter insolubles, the filtered liquid temperature is cooled to about 48 ℃, with NaOH adjust pH to 7, under constant temperature stirs, the bromeline that adds 3g, enzymolysis 5 hours, 100 ℃ of enzyme 5min that go out are through vacuum concentration, last spraying drying obtains the collagen active peptide powder.
Embodiment 4
Get cod skin 6kg fresh or that thaw, clean 4-5 time, drain with 24L water; Water intaking 12L adds the Ca (OH) of entry weight 0.1%
2Make mixed solution with 0.2% NaOH, cod skin is put in the soak solution soaked 8.5 hours, remove by filter supernatant liquor, the throw out water cleans repeatedly and makes its pH value to neutral, the Glacial acetic acid 12L that adds concentration 0.4%, soaked 2 hours, and removed by filter supernatant liquor, the throw out water cleans the pH value repeatedly to neutral.Distilled water is heated to 75 ℃, under constant temperature stirs, the distilled water extracting of adding 12L 9 hours, remove by filter insolubles, the filtered liquid temperature is cooled to about 48 ℃, with NaOH adjust pH to 7, under constant temperature stirs, the neutral protease that adds 6g, enzymolysis 4 hours, 100 ℃ of enzyme 5min that go out are through vacuum concentration, last spraying drying obtains the collagen active peptide powder.
Claims (3)
1, a kind of preparation method of cod collagen peptide, it is characterized in that: choosing cod skin is raw material, use the clear water wash clean, add calcium hydroxide and sodium hydroxide mixed liquid dipping 8-14 hour, the mixed liquid concentration of calcium hydroxide and Qing Yangization Na is 0.2-0.5%, wherein the weight ratio of calcium hydroxide and Qing Yangization Na is 1: 2-4, use the clear water repetitive scrubbing then, and make its pH value to neutral; Adding concentration is that the dilute acid solution of 0.3-0.6% soaked 1-3 hour, uses the clear water repetitive scrubbing again, makes its pH value to neutral; With the fish-skin fragmentation, in 70-80 ℃ of hot water, stir, extracting 8-10 hour, extract collagen protein, remove by filter insolubles; It is neutral that the collagen protein extracting solution is regulated pH value, under agitation condition, adds enzyme and carries out enzymolysis 3-5 hour, and hydrolysis temperature is 40-50 ℃, and enzymolysis solution goes out after enzyme, the filtration, through vacuum concentration, and spraying drying, acquisition collagen peptide powder.
2, the preparation method of a kind of cod collagen peptide according to claim 1 is characterized in that: the dilute acid solution of described immersion cod skin is a glacial acetic acid solution.
3, the preparation method of a kind of cod collagen peptide according to claim 1 and 2 is characterized in that: the used enzyme of described collagen protein enzymolysis extracting solution is a kind of in neutral protease, Sumizyme MP or the bromeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101596172A CN101407834A (en) | 2008-11-05 | 2008-11-05 | Method for preparing collagen peptide by using cod skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101596172A CN101407834A (en) | 2008-11-05 | 2008-11-05 | Method for preparing collagen peptide by using cod skin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101407834A true CN101407834A (en) | 2009-04-15 |
Family
ID=40571045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101596172A Pending CN101407834A (en) | 2008-11-05 | 2008-11-05 | Method for preparing collagen peptide by using cod skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101407834A (en) |
Cited By (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217699A (en) * | 2010-04-19 | 2011-10-19 | 浙江海洋学院 | Preparation method of squid skin protein active peptide |
| CN101870995B (en) * | 2009-04-21 | 2013-11-20 | 陈栋梁 | Process for preparing collagen tripeptide |
| CN103445189A (en) * | 2013-09-02 | 2013-12-18 | 青岛贝尔特生物科技有限公司 | Special marine albumen powder for middle-aged and old people and preparation method thereof |
| CN103611154A (en) * | 2013-11-22 | 2014-03-05 | 中华人民共和国舟山出入境检验检疫局 | Lophius litulon skin collagen peptide composition capable of resisting gram negative bacteria |
| CN103911415A (en) * | 2014-04-10 | 2014-07-09 | 广州康洛信生物科技有限公司 | Method for safely and efficiently preparing collagen via one-step process |
| CN103966295A (en) * | 2012-07-26 | 2014-08-06 | 金利食安科技股份有限公司 | Method for extracting hydrolysate of animal components by combining pressure and enzyme |
| CN104046965A (en) * | 2014-05-27 | 2014-09-17 | 中国电子科技集团公司第四十八研究所 | Radiant heating element, radiant heater and MOCVD reactor |
| CN104152518A (en) * | 2014-05-14 | 2014-11-19 | 浙江海洋学院 | Preparation method of hepatopathy complementary-food cod skin collagen peptide |
| CN104672324A (en) * | 2015-03-06 | 2015-06-03 | 荣成泰祥食品股份有限公司 | Decoloration and deodorization method of marine active collagen peptide enzymatic hydrolysate |
| CN104774258A (en) * | 2015-04-27 | 2015-07-15 | 山东美佳集团有限公司 | Preparation method of turbot fish skin collagen |
| CN105821109A (en) * | 2016-05-16 | 2016-08-03 | 青岛海博瑞克生物科技有限公司 | Pollock fishskin collagen peptide and application thereof |
| CN105963686A (en) * | 2016-05-19 | 2016-09-28 | 青岛海博瑞克生物科技有限公司 | Application of collagen peptide in preparing medicine for preventing or treating osteoporosis |
| CN105985997A (en) * | 2015-08-11 | 2016-10-05 | 慈晓丽 | Method for extracting collagen peptide from wood frog skin |
| CN107581618A (en) * | 2017-07-31 | 2018-01-16 | 浦江县昂宝生物技术有限公司 | A kind of compound based on Ba Sha fish-skin functional protein peptides |
| CN107836607A (en) * | 2017-11-28 | 2018-03-27 | 广州颜如玉医药科技有限公司 | A kind of fish skin collagen oligopeptide blueberries drink and preparation method thereof |
| CN107950933A (en) * | 2017-11-28 | 2018-04-24 | 广州颜如玉医药科技有限公司 | A kind of marine fish skin collagen oligopeptide oral liquid and preparation method thereof |
| CN108276489A (en) * | 2018-04-09 | 2018-07-13 | 无限极（中国）有限公司 | Collagen and preparation method thereof, application |
| CN109122841A (en) * | 2018-08-04 | 2019-01-04 | 天津科技大学 | The color protecting method of gadus polypeptide anti-enzymatic regent and potato fresh slices |
| CN109645285A (en) * | 2017-10-11 | 2019-04-19 | 天津威康医疗用品有限公司 | A kind of gadus peptide solid beverage |
| CN109722461A (en) * | 2019-03-14 | 2019-05-07 | 舜甫科技(固安)有限公司 | A kind of deep-sea fish collagen peptide and its production method |
| CN110720636A (en) * | 2019-11-18 | 2020-01-24 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
| CN110755293A (en) * | 2019-12-16 | 2020-02-07 | 卢婷婷 | Preparation method of antioxidant and anti-wrinkling composite moisturizing cream |
| CN111235072A (en) * | 2020-03-26 | 2020-06-05 | 新食代布丁(上海)智能科技有限公司 | Preparation method of lactobacillus plantarum for reducing fat |
| CN112335888A (en) * | 2020-11-09 | 2021-02-09 | 四川必信科技有限公司 | Sea cucumber and abalone oligopeptide powder and preparation method thereof |
| CN112481343A (en) * | 2021-01-14 | 2021-03-12 | 山东恒鑫生物科技有限公司 | Method for producing collagen peptide by using cod skin raw material |
| CN112715959A (en) * | 2021-01-28 | 2021-04-30 | 海南省胜鑫生物科技有限责任公司 | Cod small-molecule collagen peptide vitamin C powder |
| CN112715813A (en) * | 2020-12-26 | 2021-04-30 | 安徽尚美轩生物技术有限公司 | Cod collagen peptide beverage and preparation method thereof |
| CN112715815A (en) * | 2021-01-28 | 2021-04-30 | 海南省胜鑫生物科技有限责任公司 | Cod micromolecular collagen peptide solid beverage |
| CN113201064A (en) * | 2020-10-09 | 2021-08-03 | 海南翔泰生物科技有限公司 | Method for preparing fish skin collagen powder from fresh fish skin |
| CN113652459A (en) * | 2021-05-18 | 2021-11-16 | 延边大学 | Cod skin collagen peptide and extraction method thereof |
| CN113755550A (en) * | 2021-08-31 | 2021-12-07 | 海南华研胶原科技股份有限公司 | A kind of marine fish oligopeptide prepared by using deep-sea cod skin as raw material and preparation method thereof |
| CN117534748A (en) * | 2024-01-10 | 2024-02-09 | 德州蓝力生物技术有限公司 | Preparation method of beauty-maintaining Yan Yujiao original protein peptide |
-
2008
- 2008-11-05 CN CNA2008101596172A patent/CN101407834A/en active Pending
Cited By (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870995B (en) * | 2009-04-21 | 2013-11-20 | 陈栋梁 | Process for preparing collagen tripeptide |
| CN102217699A (en) * | 2010-04-19 | 2011-10-19 | 浙江海洋学院 | Preparation method of squid skin protein active peptide |
| CN102217699B (en) * | 2010-04-19 | 2012-08-22 | 浙江海洋学院 | Preparation method of squid skin protein active peptide |
| CN103966295A (en) * | 2012-07-26 | 2014-08-06 | 金利食安科技股份有限公司 | Method for extracting hydrolysate of animal components by combining pressure and enzyme |
| CN103445189A (en) * | 2013-09-02 | 2013-12-18 | 青岛贝尔特生物科技有限公司 | Special marine albumen powder for middle-aged and old people and preparation method thereof |
| CN103611154A (en) * | 2013-11-22 | 2014-03-05 | 中华人民共和国舟山出入境检验检疫局 | Lophius litulon skin collagen peptide composition capable of resisting gram negative bacteria |
| CN103911415A (en) * | 2014-04-10 | 2014-07-09 | 广州康洛信生物科技有限公司 | Method for safely and efficiently preparing collagen via one-step process |
| CN103911415B (en) * | 2014-04-10 | 2016-06-15 | 广州康洛信生物科技有限公司 | Single stage method prepares the method for collagen protein |
| CN104152518A (en) * | 2014-05-14 | 2014-11-19 | 浙江海洋学院 | Preparation method of hepatopathy complementary-food cod skin collagen peptide |
| CN104152518B (en) * | 2014-05-14 | 2017-01-11 | 浙江海洋学院 | Preparation method of hepatopathy complementary-food cod skin collagen peptide |
| CN104046965A (en) * | 2014-05-27 | 2014-09-17 | 中国电子科技集团公司第四十八研究所 | Radiant heating element, radiant heater and MOCVD reactor |
| CN104672324A (en) * | 2015-03-06 | 2015-06-03 | 荣成泰祥食品股份有限公司 | Decoloration and deodorization method of marine active collagen peptide enzymatic hydrolysate |
| CN104774258A (en) * | 2015-04-27 | 2015-07-15 | 山东美佳集团有限公司 | Preparation method of turbot fish skin collagen |
| CN105985997A (en) * | 2015-08-11 | 2016-10-05 | 慈晓丽 | Method for extracting collagen peptide from wood frog skin |
| CN105821109A (en) * | 2016-05-16 | 2016-08-03 | 青岛海博瑞克生物科技有限公司 | Pollock fishskin collagen peptide and application thereof |
| CN105963686A (en) * | 2016-05-19 | 2016-09-28 | 青岛海博瑞克生物科技有限公司 | Application of collagen peptide in preparing medicine for preventing or treating osteoporosis |
| CN107581618A (en) * | 2017-07-31 | 2018-01-16 | 浦江县昂宝生物技术有限公司 | A kind of compound based on Ba Sha fish-skin functional protein peptides |
| CN109645285A (en) * | 2017-10-11 | 2019-04-19 | 天津威康医疗用品有限公司 | A kind of gadus peptide solid beverage |
| CN107950933A (en) * | 2017-11-28 | 2018-04-24 | 广州颜如玉医药科技有限公司 | A kind of marine fish skin collagen oligopeptide oral liquid and preparation method thereof |
| CN107836607A (en) * | 2017-11-28 | 2018-03-27 | 广州颜如玉医药科技有限公司 | A kind of fish skin collagen oligopeptide blueberries drink and preparation method thereof |
| CN108276489A (en) * | 2018-04-09 | 2018-07-13 | 无限极（中国）有限公司 | Collagen and preparation method thereof, application |
| CN109122841A (en) * | 2018-08-04 | 2019-01-04 | 天津科技大学 | The color protecting method of gadus polypeptide anti-enzymatic regent and potato fresh slices |
| CN109722461A (en) * | 2019-03-14 | 2019-05-07 | 舜甫科技(固安)有限公司 | A kind of deep-sea fish collagen peptide and its production method |
| CN109722461B (en) * | 2019-03-14 | 2022-05-10 | 舜甫科技(固安)有限公司 | Deep sea fish collagen peptide and production method thereof |
| CN110720636A (en) * | 2019-11-18 | 2020-01-24 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
| CN110755293A (en) * | 2019-12-16 | 2020-02-07 | 卢婷婷 | Preparation method of antioxidant and anti-wrinkling composite moisturizing cream |
| CN111235072A (en) * | 2020-03-26 | 2020-06-05 | 新食代布丁(上海)智能科技有限公司 | Preparation method of lactobacillus plantarum for reducing fat |
| CN113201064A (en) * | 2020-10-09 | 2021-08-03 | 海南翔泰生物科技有限公司 | Method for preparing fish skin collagen powder from fresh fish skin |
| CN112335888A (en) * | 2020-11-09 | 2021-02-09 | 四川必信科技有限公司 | Sea cucumber and abalone oligopeptide powder and preparation method thereof |
| CN112715813A (en) * | 2020-12-26 | 2021-04-30 | 安徽尚美轩生物技术有限公司 | Cod collagen peptide beverage and preparation method thereof |
| CN112481343B (en) * | 2021-01-14 | 2022-03-18 | 山东恒鑫生物科技有限公司 | Method for producing collagen peptide by using cod skin raw material |
| CN112481343A (en) * | 2021-01-14 | 2021-03-12 | 山东恒鑫生物科技有限公司 | Method for producing collagen peptide by using cod skin raw material |
| CN112715815A (en) * | 2021-01-28 | 2021-04-30 | 海南省胜鑫生物科技有限责任公司 | Cod micromolecular collagen peptide solid beverage |
| CN112715959A (en) * | 2021-01-28 | 2021-04-30 | 海南省胜鑫生物科技有限责任公司 | Cod small-molecule collagen peptide vitamin C powder |
| CN113652459A (en) * | 2021-05-18 | 2021-11-16 | 延边大学 | Cod skin collagen peptide and extraction method thereof |
| CN113755550A (en) * | 2021-08-31 | 2021-12-07 | 海南华研胶原科技股份有限公司 | A kind of marine fish oligopeptide prepared by using deep-sea cod skin as raw material and preparation method thereof |
| CN113755550B (en) * | 2021-08-31 | 2024-07-26 | 海南华研胶原科技股份有限公司 | Marine fish oligopeptide prepared from deep-sea cod skin as raw material and preparation method thereof |
| CN117534748A (en) * | 2024-01-10 | 2024-02-09 | 德州蓝力生物技术有限公司 | Preparation method of beauty-maintaining Yan Yujiao original protein peptide |
| CN117534748B (en) * | 2024-01-10 | 2024-03-12 | 德州蓝力生物技术有限公司 | Preparation method of beauty-maintaining Yan Yujiao original protein peptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101407834A (en) | Method for preparing collagen peptide by using cod skin | |
| CN101092449B (en) | Technique for preparing collagen of freshwater fish skin | |
| CN101307348B (en) | Method for preparing undenatured collagen from fish scale of fresh water fish | |
| CN102690370B (en) | Comprehensive utilization technique of marine fish bones | |
| CN101418328A (en) | Method for producing fish scale collagen protein | |
| CN1100490C (en) | Separation and extraction process of effective component in resource insect | |
| CN103773830B (en) | A kind of method extracting collagen protein from fish scale | |
| CN101570772B (en) | Method for preparing natural ossein | |
| CN102154421A (en) | Method for extracting collagen from fish processing waste including skin, bones and scale | |
| CN103184262B (en) | Method for extracting and purifying pepsin-soluble collagens of black sea cucumber in East China Sea | |
| CN102550824B (en) | Method for producing small peptide amino acid microelement chelate by way of acid hydrolysis of protein | |
| CN102550900A (en) | Method for preparing edible gelatin by means of freshwater fish skin | |
| CN103601802A (en) | Method for promoting fishbone hydrolysis utilization | |
| CN103243143A (en) | Method for extracting hydrolyzed collagen by using bone meal | |
| CN102558345A (en) | Method for extracting collagen from sturgeon skin | |
| CN102924624A (en) | Method for preparing chondroitin sulfate from sturgeon cartilage | |
| CN103627768A (en) | Fish skin collagen bioactive small peptide and preparation method thereof | |
| CN104905256A (en) | Composite amino acid chelated calcium prepared by using oysters as raw materials | |
| CN105274171A (en) | Method of preparing antioxidant peptides by hydrolyzing skin of tilapias using compound protease | |
| CN101250218A (en) | Preparation method of cod skin collagen | |
| CN100497651C (en) | Sturgon chondrine extracting process | |
| CN106632665A (en) | Method for extracting collagen from freshwater fish skin by lactic acid | |
| CN101322541B (en) | Method for preparing delicate flavor agent using fresh water fish leftover bits and pieces | |
| CN102936611A (en) | Collagen extracted from black butterflyfish skins with scales and application of collagen | |
| CN1683550A (en) | Collagen and its preparation process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090415 |