CN101406292B - Black-bone chicken gastrodia tuber nutrient fluid - Google Patents

Abstract

The invention relates to a Taihe chicken and rhizoma gastrodiae nutrient solution, which belongs to the field of traditional Chinese medicine health care product for strengthening the immunity. Each 100 milliliters of the Taihe chicken and rhizoma gastrodiae nutrient solution is prepared by the following raw materials by the prior method: 19 milliliters of rhizoma gastrodiae extract, 68 milliliters of Taihe chicken hydrolysate, 6 grams of honey, 0.15 gram of citric acid and 0.075 gram of glycosylated protein. The content of gastrodine with a molecular formula of C13H18O7 in per 100 milliliters of the Taihe chicken and rhizoma gastrodiae nutrient solution is not less than 31 milligrams; and the content of amino nitrogen is not less than 800 milligrams. The Taihe chicken hydrolysate in the Taihe chicken and rhizoma gastrodiae nutrient solution is obtained by the hydrolysis process; and the rhizoma gastrodiae extract is obtained by the extraction process and the clarification process. The Taihe chicken and rhizoma gastrodiae nutrient solution has the function of strengthening the immunity of the organism, has stable product quality and high product development value, and can be stored for as long as two years.

Description

A kind of black-bone chicken Gastrodia elata nutrient solution

Technical field:

The invention relates to a black-bone chicken Gastrodia elata nutrient solution, which belongs to the technical field of traditional Chinese medicine health products for enhancing immunity.

Background technique:

Immunity is the body's ability to resist external pathogenic factors and maintain a stable internal environment. Low immunity can prevent the immune system from functioning normally, making it susceptible to infections such as bacteria, viruses, and fungi that can induce diseases. Therefore, improving the body's immunity and enhancing the body's immune function is the key to preventing the occurrence of various diseases and recovering patients. Among the many measures to enhance the body's immune function, nutritional regulation is the most feasible and at the core.

Black-bone chicken, also known as black-bone chicken, is a rare medicinal bird unique to my country. Scientific research proves that: black-bone chicken has higher amino acid content, especially 8 kinds of essential amino acid content are higher than other chicken species, such as glutamic acid 14.75%, aspartic acid 8.97%, lysine 8.08%, leucine 7.61% , alanine 5.37%, tyrosine 5.37%, arginine 5.25%, etc. These amino acids are very easy to synthesize human protein, constitute human cells, and are important raw materials for human immune substances, enzymes, hormones, etc. In addition, black-bone chicken is also very rich in trace elements. Calcium, sodium, potassium, iron, copper, zinc, manganese, vitamins, etc. are widely distributed in tissues and organs. The iron content of the edible part is 70-80mg/kg, which is higher than Regular chicken is 45% higher.

Gastrodia elata Blume (Gastrodia elata Blume) is the dry tuber of Gastrodia elata, a perennial parasitic plant of Orchidaceae, and it is a valuable Chinese medicinal material in my country. Gastrodia elata contains not only gastrodin, gastrodin, vanillyl alcohol, vanilla aldehyde, parisin, citric acid, succinic acid and other active substances, but also amino acids and trace elements necessary for human body. Gastrodia elata has the effect of calming the liver, relieving wind and relieving convulsions, and is often used to treat headaches, dizziness, and limb numbness. In recent years, studies have found that Gastrodia elata also has various physiological effects such as enhancing immune function, lowering blood pressure, promoting intelligence, and resisting senile dementia.

Both black-bone chicken and Gastrodia elata belong to folk nourishing recipes, which have health functions such as nourishing qi and blood, benefiting essence, nourishing liver and improving eyesight, and have a long history of application as folk nourishing food. Such as gastrodia steam pot chicken, gastrodia black chicken soup, gastrodia black chicken wine and so on. However, there is no report on the use of modern food processing technology to extract the active ingredients of Gastrodia elata and black-bone chicken to produce health food and to study its nutritional efficacy and mechanism of action.

Invention content:

The object of the present invention is to provide a black-bone chicken Gastrodia elata nutrient solution capable of enhancing body immunity, stable product quality and long storage period.

The invention uses black-bone chicken and Gastrodia elata as raw materials, utilizes modern food processing technology, extracts the active ingredients of Gastrodia elata to prepare Gastrodia elata extract, and uses enzymatic hydrolysis to prepare the active peptide of black-bone chicken egg white, and develops the nutrient solution of black-bone chicken Gastrodia elata after scientific and reasonable compatibility. According to the "Technical Specifications for Inspection and Evaluation of Health Food" promulgated by the Ministry of Health in 2003, the regulating effect of the nutrient solution on the immune function of the body was studied.

The present invention is achieved like this:

Each 100mL black-bone chicken Gastrodia elata nutrient solution is made of the following raw materials: take black-bone chicken Gastrodia elata extract 19mL, black-bone chicken gastrodia elata 68mL, honey 6g, citric acid 0.15g, protein sugar 0.075g, water, mix well, and sterilize at 80°C for 10min After the treatment, the black-bone chicken Gastrodia elata nutrient solution is obtained. Among them, the content of gastrodin with molecular formula C ₁₃ H ₁₈ O ₇ per 100 mL of black-bone chicken gastrodia nutrient solution is not less than 31 mg, and the content of amino nitrogen is not less than 800 mg.

The amino acid hydrolyzate of black-bone chicken in the present invention is prepared by the hydrolysis process whose patent number is 2006100110399: black-bone chicken (remove bone and viscera) is pre-steamed for 20 minutes, mashed for 2 minutes by a multifunctional pulverizer, water of 2 times the weight is added, and papain is added as the base 5.3% (W/W) of the substrate, hydrolyze at 65°C for 3h, inactivate the enzyme at 85°C for 20min, then add neutral protease as 10.7% (W/W) of the substrate, hydrolyze at 45°C for 3h, and inactivate at 85°C Enzyme for 20 minutes, followed by filtration and flocculation treatment with 0.2% (W/V) chitosan to obtain a clear and transparent black-bone chicken amino acid hydrolyzate.

Gastrodia elata extract in the present invention is prepared by extraction and clarification process: Gastrodia elata is crushed through an 80-mesh sieve to obtain Gastrodia elata powder, dissolved in 50% ethanol at a ratio of 1:10 (W/V), and placed at 70°C for rotation Extract in a water bath in the evaporator for 3 times, extract for 1 hour each time, filter and combine the filtrates to obtain the Gastrodia elata extract; concentrate the Gastrodia elata extract to a ratio of crude drug to medicinal liquid of 1:13 (W/V), add 0.15% (W/V /V) chitosan, after fully stirring, at 25°C, let it stand for 12h to obtain a clear and transparent Gastrodia elata extract.

The black-bone chicken gastrodia nutrient solution of the invention is a clear and transparent tan liquid, has the unique flavor of gastrodia elata and the aroma of chicken, is sweet and sour, slightly bitter, has no peculiar smell, and has no precipitation and impurities visible to the naked eye.

The inventor adopts conventional methods to study the quality index and product stability of the black-bone chicken Gastrodia elata nutrient solution. The results showed that the sensory indicators, main quality indicators (gastrodin, amino nitrogen) and microbial indicators (total number of colonies, number of mold, yeast and the closest approximation of coliforms) of the black-bone chicken Gastrodia elata nutrient solution all met the expected product quality requirements. Stored for three months at 37° C. and a relative humidity of 75%, the quality of the black-bone chicken Gastrodia elata nutrient solution is stable, and the gastrodin contained in it is ≥31 mg/100 mL, and the amino nitrogen is ≥ 800 mg/100 mL.

In accordance with the 2003 "Health Food Functional Evaluation Procedures and Test Method Specifications", the inventor conducted animal experiments on the function of the black-bone chicken Gastrodia elata nutrient solution to enhance the body's immunity. The results showed that: the nutrient solution could significantly increase the mouse thymus/body weight ratio (P<0.05); significantly increase the number of hemolytic plaques in the mouse spleen cells (P<0.01), and the higher the dose, the more significant the effect; Both 20-fold and 30-fold dose groups could significantly enhance the activity of NK cells in mice (P<0.05). It is proved that the nutrient solution of black-bone chicken Gastrodia elata has the effect of enhancing the immune function of the body.

The invention has the advantages of enhancing the immune function of the body, stable product quality, a storage period of up to two years, and high product development value.

Detailed ways:

Each 100mL black-bone chicken Gastrodia elata nutrient solution is made of the following raw materials: Take black-bone chicken Gastrodia elata extract 19mL, black-bone chicken gastrodia elata 68mL, honey 6g, citric acid 0.15g, protein sugar 0.075g, water, mix well, and pack at 80°C for 10min After the sterilization treatment, the black-bone chicken Gastrodia elata nutrient solution was obtained. Wherein, the content of gastrodin with molecular formula C ₁₃ H ₁₈ O ₇ in every 100 mL of black-bone chicken Gastrodia elata nutrient solution is not less than 31 mg, and the content of amino nitrogen is not less than 800 mg.

The black-bone chicken hydrolyzate among the present invention adopts the patent No. to be the hydrolysis process of 2006100110399 and clarification process preparation: get the black-bone chicken and pre-steam 20min, mash 2min through the multifunctional pulverizer, add the water of 2 times of weight, add 5.3% (W /W) Papain was hydrolyzed at 65°C for 3h, enzyme was inactivated at 85°C for 20min, then 10.7% (W/W) neutral protease was added, hydrolyzed at 45°C for 3h, enzyme was inactivated at 85°C for 20min, and the black-bone chicken hydrolyzed solution was obtained by filtration , after being flocculated with 0.2% (W/V) chitosan, a clear and transparent black-bone chicken hydrolyzate was obtained.

Gastrodia elata extract in the present invention is prepared by extraction process and clarification process: Gastrodia elata is crushed through an 80-mesh sieve to obtain Gastrodia elata powder, which is dissolved by adding 50% ethanol in a ratio of 1:10 (W/V), and placed in 70 °C rotary evaporator water bath extraction 3 times, each extraction 1h, after filtering and combining the filtrates to obtain Gastrodia elata extract. Concentrate the Gastrodia elata extract until the ratio of crude drug to medicinal liquid is 1:13 (W/V), add 0.15% (W/V) chitosan, stir well, and let it stand for 12 hours at 25°C to obtain clarification Transparent gastrodia elata extract.

The equipment used in the present invention is conventional equipment, mainly including a rotary evaporator, a digital display constant temperature water bath and a water-isolated electric heating constant temperature incubator.

The recommended dosage for the human body of the black-bone chicken Gastrodia elata nutrient solution of the present invention is 50 mL, 1-2 times a day. The nutrient solution has been proved by animal experiments to enhance the immune function of the body.

Claims (1)

1. A black-bone chicken gastrodia elata nutrient solution, characterized in that: every 100mL black-bone chicken gastrodia elata nutrient solution is made of the following raw materials: black-bone chicken hydrolyzate 68mL, gastrodia elata extract 19mL, honey 6g, citric acid 0.15g, protein sugar 0.075g; every 100mL The content of gastrodin with the molecular formula C ₁₃ H ₁₈ O ₇ in the black-bone chicken Gastrodia elata nutrient solution is not less than 31 mg per 1 mL, and the content of amino nitrogen is not less than 800 mg; where: a. The black-bone chicken hydrolyzate is obtained through the following process: the black-bone chicken of deboning and viscera is pre-steamed for 20 minutes, mashed for 2 minutes by a multifunctional pulverizer, water with 2 times of weight is added, and papain is added as 5.3% (W/W) of the substrate ), hydrolyze at 65°C for 3h, inactivate the enzyme at 85°C for 20min, then add neutral protease as 10.7% (W/W) of the substrate, hydrolyze at 45°C for 3h, inactivate the enzyme at 85°C for 20min, then filter, pass through 0.2 % (W/V) chitosan flocculation treatment to obtain clear and transparent black-bone chicken hydrolyzate; b. Gastrodia elata extract is obtained through the following process by extraction process and clarification process: Gastrodia elata is obtained Gastrodia elata powder after being broken through 80 mesh sieves, adds 50% ethanol to dissolve in the ratio of 1: 10 (W/V), it is placed in 70 ℃ rotary evaporator water bath extraction 3 times, each extraction 1h, after filtering and merging the filtrates, Gastrodia elata extract was obtained; the Gastrodia elata extract was concentrated until the ratio of crude drug to medicinal liquid was 1:13 (W/V), and 0.15% ( W/V) chitosan, after fully stirring, at 25°C, let stand for 12h to obtain a clear and transparent Gastrodia elata extract.