CN101376906A - Production method for mixed nucleotides - Google Patents
Production method for mixed nucleotides Download PDFInfo
- Publication number
- CN101376906A CN101376906A CNA2008101554841A CN200810155484A CN101376906A CN 101376906 A CN101376906 A CN 101376906A CN A2008101554841 A CNA2008101554841 A CN A2008101554841A CN 200810155484 A CN200810155484 A CN 200810155484A CN 101376906 A CN101376906 A CN 101376906A
- Authority
- CN
- China
- Prior art keywords
- acid
- mixed nucleotides
- solution
- filtration
- production method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002773 nucleotide Substances 0.000 title claims abstract description 39
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 39
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- 239000012535 impurity Substances 0.000 claims abstract description 18
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 10
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 10
- 239000011734 sodium Substances 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims abstract description 7
- 229950006790 adenosine phosphate Drugs 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 5
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 5
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 claims abstract description 4
- DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 claims abstract description 4
- IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 claims abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 239000000047 product Substances 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 7
- 239000000049 pigment Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 3
- 239000004226 guanylic acid Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 108091028664 Ribonucleotide Proteins 0.000 abstract 3
- 239000002336 ribonucleotide Substances 0.000 abstract 3
- 125000002652 ribonucleotide group Chemical group 0.000 abstract 3
- RQFCJASXJCIDSX-UHFFFAOYSA-N 14C-Guanosin-5'-monophosphat Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(O)=O)C(O)C1O RQFCJASXJCIDSX-UHFFFAOYSA-N 0.000 abstract 1
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 abstract 1
- 235000013928 guanylic acid Nutrition 0.000 abstract 1
- 238000001471 micro-filtration Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- -1 particularly Chemical group 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Landscapes
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a production method of mixed nucleotide, which subsequently comprises the following steps: RNA solution preparation; RNA solution zymohydrolysis; filtration; decoloring by a decoloring column; sodium filtration-concentration. The process conditions of the zymohydrolysis reaction of the RNA solution is strictly controlled, so that the total area of four main peaks 5'-adenylic acid, 5'-cytidylic acid, 5'-uridylic acid and 5'-guanylic acid in the high-pressure liquid chromatograph of the obtained ribonucleotide mixture reaches about 95 percent of the total peak area; solid particles are removed by filtration; impurities with smaller grain size are removed by micro-filtration; the product is decolored by the decoloring column, and the residual protein and other impurities in the solution are removed at the same time; partial water and impurities with small molecular weight are removed by sodium filtration-concentration. The purity of the solution after once and again purification is rather high, and the concentrated solution can be used as a mixed ribonucleotide product or receives further spray drying treatment to obtain mixed ribonucleotide powder. The method has the advantages of short process route, high yield, low cost, less waste discharge, high product content and good safety.
Description
Technical field
The present invention relates to a kind of production method of mixed nucleotides, particularly, Nucleotide prepares by enzyme process.
Background technology
In the market, Nucleotide is as nutritious prod, and the subject of a sale is mainly baby and old man.Nucleotide for baby has strict quality standard, is to be added in the infant formula after being mixed by a certain percentage with the mononucleotide through the chromatography post crystallization again.And just do not have standard as the Nucleotide quality of health care for the aged product.Once it is dark in strong tea to see liquid state " Nucleotide " color that is contained in the ampere bottle; Also once " Nucleotide " capsule of certain brand was carried out mass analysis, wherein the nucleotide content less than 10%.
Summary of the invention
Technical problem to be solved by this invention provides a kind of production method of mixed nucleotides, and is with short production cycle, energy consumption is little, environmental pollution is little, security is good, product yield is high, production cost is low.
For solving above technical problem, the present invention takes following technical scheme:
A kind of production method of mixed nucleotides may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to neutrality, add 5 '-phosphodiesterase at a certain temperature, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring, above-mentioned filtrate is by decolorizing column, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is by the sodium filter membrane, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product.
If target product is a solid, then the concentrated solution of above-mentioned steps (5) gained can further spray-dried acquisition mixed nucleotides powder.
The present invention compares with the method that obtains mixed nucleotides that is mixed by a certain percentage with mononucleotide, because the process of manufacture order Nucleotide except the needs removal of impurity, also will be passed through chromatography with the enzymolysis solution of Yeast Nucleic Acid, is separated into the solution of four kinds of mononucleotides; Concentrate respectively then, crystallization, drying, this is a very long and complicated process.By comparison, technical scheme of the present invention has following advantage:
Production cycle is about 1/3rd of mononucleotide technology; Energy consumption is about 1/2nd of mononucleotide technology; Blowdown is about 1/2nd of mononucleotide technology; Security is good; Product yield increases substantially; Production cost descends significantly; The investment of founding the factory also descends significantly.
Embodiment
Below the specific embodiment of the present invention is described:
Production method according to the mixed nucleotides of present embodiment may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to about pH7, about 70 ℃, add 5 '-phosphodiesterase, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring, above-mentioned filtrate is by being equipped with the decolorizing column of acid decolorizing resin or granulated active carbon, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is the sodium filter membrane of 2 nanometers by mean pore size, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product;
(6), spraying drying: the further spray-dried acquisition mixed nucleotides powder of concentrated solution.
At first under the prerequisite of strictness control enzymolysis liquid of ribonuclease quality, remove solid impurity after filtration, obtain limpid, transparent but with the enzymolysis solution of raw material Yeast Nucleic Acid inherent pigment; This enzymolysis solution is through decolorizing column, sloughs the pigment that brought by raw material Yeast Nucleic Acid and the protein and some impurity of solubilised state, and the quality of enzymolysis filtrate further promotes, and obtains being water white destainer; Destainer is that the sodium filter membrane of 2 nanometers is removed small molecular weight impurity remaining in portion water and the destainer by mean pore size again.In the spissated while, solution is further purified.
By above quality control process, at the character of the impurity that may exist in the enzymolysis solution, adopt various mode progressively to remove impurity, obtain high-quality sodium filter concentration liquid; Spray-dried again, just obtain high-quality mixed nucleotides powder, the technology of production Nucleotide of the present invention, Nucleotide can be used as nutritious prod.This operational path is short, the yield height, and cost is low, and blowdown is few; The product content height, color is white, and quality is good, and the ratio of four kinds of mononucleotides is the ratio in the natural nucleotide, is the most rational, thus for formulating the quality standard of mixed nucleotides, condition has been created in standard nucleotide nutrient product market.
Claims (2)
1, a kind of production method of mixed nucleotides is characterized in that: may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to neutrality, add 5 '-phosphodiesterase, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring: above-mentioned filtrate is by decolorizing column, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is by the sodium filter membrane, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product.
2, the production method of a kind of mixed nucleotides according to claim 1 is characterized in that: this production method also comprises step (6): by the spray-dried mixed nucleotides powder that makes of above-mentioned steps (5) gained concentrated solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101554841A CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101554841A CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101376906A true CN101376906A (en) | 2009-03-04 |
Family
ID=40420645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101554841A Pending CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101376906A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331035A (en) * | 2013-06-28 | 2013-10-02 | 南京工业大学 | Method for decoloring nucleotide enzymolysis liquid |
| CN107529809A (en) * | 2015-03-06 | 2018-01-02 | 内蒙古伊利实业集团股份有限公司 | Polynucleotide composition and its application in food |
-
2008
- 2008-10-06 CN CNA2008101554841A patent/CN101376906A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331035A (en) * | 2013-06-28 | 2013-10-02 | 南京工业大学 | Method for decoloring nucleotide enzymolysis liquid |
| CN107529809A (en) * | 2015-03-06 | 2018-01-02 | 内蒙古伊利实业集团股份有限公司 | Polynucleotide composition and its application in food |
| CN107529809B (en) * | 2015-03-06 | 2020-03-13 | 内蒙古伊利实业集团股份有限公司 | Nucleotide composition and application thereof in food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109593034B (en) | Method for preparing shikimic acid from ginkgo leaf extraction waste liquid | |
| CN101486637A (en) | Method for extracting amber acid from fermentation liquor | |
| CN102329340A (en) | Method for preparing D-mannose | |
| CN101863783A (en) | Method for separating and purifying gamma-aminobutyric acid from glutamic decarboxylase enzymatic hydrolyzate | |
| CN111087296A (en) | Method for extracting shikimic acid and shikimic acid extract | |
| CN112979482A (en) | High-purity L-valine and preparation method and application thereof | |
| CN100507007C (en) | Production method of natural colored sugar | |
| CN108558909B (en) | The method of effective component in synthetical recovery clavulanic acid tert-butylamine salt crystalline mother solution | |
| CN103508933B (en) | Separating and purifying method for L-tryptophan | |
| KR100828706B1 (en) | Purification Method of 5'-inosinic Acid Fermentation Solution Using Crystallization Process | |
| CN102321137A (en) | Preparation method of adenosylcobalamin | |
| US20220204522A1 (en) | Process for separating and purifying artemisinin | |
| CN101376906A (en) | Production method for mixed nucleotides | |
| CN103420826A (en) | Method for extracting succinic acid from fermentation broth | |
| CN109485559B (en) | Method for extracting shikimic acid from star anise | |
| AU2010318820B2 (en) | Method for producing white sugar, light brown sugar and dark brown sugar using direct recovery process | |
| CN1321126C (en) | Process for preparing high purity acarbose | |
| CN116332822A (en) | Method for preparing low-chroma 5-hydroxytryptophan from garna seeds | |
| CN102020576A (en) | High-purity glutamic acid and preparation method thereof | |
| CN106883286B (en) | Extraction and purification method of tyrosine derivative | |
| CN102924551A (en) | Method for extracting vernine from fermentation liquor | |
| CN117050021B (en) | Method for separating and extracting tetrahydropyrimidine from fermentation liquor | |
| CN108299538B (en) | Method for removing isoursodesoxycholic acid in duck bile | |
| CN112250564B (en) | Method for extracting shikimic acid from fermentation broth | |
| CN113968794B (en) | Process method for separating and purifying glutamine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20090304 |