CN101366394B - Composite biological insecticide - Google Patents
Composite biological insecticide Download PDFInfo
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- CN101366394B CN101366394B CN2008101957043A CN200810195704A CN101366394B CN 101366394 B CN101366394 B CN 101366394B CN 2008101957043 A CN2008101957043 A CN 2008101957043A CN 200810195704 A CN200810195704 A CN 200810195704A CN 101366394 B CN101366394 B CN 101366394B
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- Prior art keywords
- composite biological
- prodenia litura
- biological insecticide
- nuclear polyhedrosis
- polyhedrosis virus
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- 239000002917 insecticide Substances 0.000 title claims abstract description 29
- 239000002131 composite material Substances 0.000 title claims abstract description 27
- 241000985245 Spodoptera litura Species 0.000 claims abstract description 46
- 239000000843 powder Substances 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 7
- CXEGAUYXQAKHKJ-NSBHKLITSA-N emamectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](NC)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 CXEGAUYXQAKHKJ-NSBHKLITSA-N 0.000 claims abstract 4
- 239000000203 mixture Substances 0.000 claims description 15
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种复合生物杀虫剂及其制备方法，属生物农药技术领域。本发明中的斜纹夜蛾核型多角体病毒苏州株1号(SpltNPV-sz-1)已于2008年6月25日保藏于中国典型培养物保藏中心，保藏号CCTCC NO：V200806。该复合生物杀虫剂按重量计，由以下成分组成：斜纹夜蛾NPV-sz-1原粉1～10％，甲氨基阿维菌素苯甲酸盐原粉0.1～3％，其它为功能性助剂和溶剂。本发明具有高效、广谱、无公害的特点，适用于防治斜纹夜蛾、小菜蛾、瓜绢螟等鳞翅目害虫及螨类，拓宽了杀虫范围，提高了杀虫效果，延缓害虫抗药性的产生，起到持续的自然控制作用，减少化学农药的用药量，降低成本，保护生态环境，应用前景十分广阔。The invention discloses a composite biological insecticide and a preparation method thereof, belonging to the technical field of biological pesticides. Spodoptera litura nucleopolyhedrosis virus Suzhou strain No. 1 (SpltNPV-sz-1) in the present invention has been preserved in China Center for Type Culture Collection on June 25, 2008, and the preservation number is CCTCC NO: V200806. The composite biological insecticide is composed of the following components by weight: Spodoptera litura NPV-sz-1 original powder 1-10%, emamectin benzoate original powder 0.1-3%, and others are functional additives and solvents. The invention has the characteristics of high efficiency, broad spectrum and no pollution, and is suitable for preventing and controlling Lepidoptera pests and mites such as Spodoptera litura, Plutella xylostella, and Sapphire Borer. The generation of medicinal properties plays a role in continuous natural control, reduces the dosage of chemical pesticides, reduces costs, and protects the ecological environment. The application prospect is very broad.
Description
Technical field
The invention belongs to the biopesticide of control pest, relate to a kind of effective active composition and be that prodenia litura nuclear polyhedrosis virus and emamectin-benzoate be composited is efficient, wide spectrum, harmless boilogical insecticide, be used to prevent and treat lepidoptera pest and mite classes such as prodenia litura, diamond-back moth, melon thin,tough silk snout moth's larva, beet armyworm, cotton bollworm.
Background technology
Along with the continuous development of national economy and the raising day by day of living standards of the people, people are more and more higher for the quality requirement of various agricultural product, and particularly the security of products problem has been subjected to paying close attention to widely and paying attention to.At present, in the preventing and controlling of various diseases and pests of agronomic crop, the situation of depending on chemical pesticide unduly is still very serious, has brought pest resistance, problem such as wildness and environmental pollution more thus, directly has influence on human food security and living environment.Therefore, greatly develop efficient, wide spectrum, with the good harmless boilogical agricultural chemicals of Environmental compatibility be the needs of agricultural sustainable development.
Prodenia litura is one of primary pest of crops, and all there is distribution domestic each provinces and regions.These worm feeding habits are extremely wide, and the known host plant that endangers reaches 389 kinds of 109 sections, comprises many vegetables, flowers and crops etc.In recent years along with the change of agricultural tillage system and the fast development of urbanization process, the cultivated area of dry crops such as vegetables, flowers, greening lawn is more and more, the harm of prodenia litura also is becoming increasingly rampant, adopt chemical control often to fall flat in the production, and cause environmental pollution easily, residue of pesticide and person poultry poisoning etc.
Prodenia litura NPV-sz-1 is a kind of causal organism, gets food by Spodoptera litura larvae and causes infection morbidity and death, and its worm that dies of illness can be caused the popular of disease in the field, can play the long-term natural control action that continues.This virus has that selectivity is strong, pathogenicity is high, to environment and the harmless characteristics of other bio-safety.Utilize prodenia litura NPV to carry out biological control, the relevant issues that can avoid above-mentioned chemical pesticide to exist, but insect is long partially from the susceptible time to death, and simultaneous other insect is not had inhibitory action.
Emamectin-benzoate (Emamectin benzoate) is a kind of never poison, the information transmission of nerve endings in the interference insect body, thereby getting in touch of block nerves tip and muscle, making insect paralysis, food refusal and death, is a kind of efficient, wide spectrum, non-harmful novel biopesticide.But this agricultural chemicals also should not reduce drug effect, and aquatile is also had certain influence easily in the long-term use continuously of areal.Therefore, provide a kind of and can not only effectively prevent and treat various pests such as prodenia litura, and have the lasting insect pest of control naturally effect, reduce the dosage of chemical pesticide, reduce cost, the novel agrochemical of preserving the ecological environment has crucial meaning.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists, provide a kind of and can not only effectively prevent and treat various pests, and can continue the composite biological insecticide of nature Pest Control.
For achieving the above object, the technical solution used in the present invention is: a kind of composite biological insecticide, its composition is: prodenia litura nuclear polyhedrosis virus content be 500～1,000 hundred million PIB/g the former powder of prodenia litura nuclear polyhedrosis virus, emamectin-benzoate by weight content be 50～95% the former powder of emamectin-benzoate, functional aid and solvent; The production bacterial strain of described prodenia litura nuclear polyhedrosis virus is a prodenia litura nuclear polyhedrosis virus Suzhou strain No.1 (SpltNPV-sz-1), this bacterial strain is deposited in Chinese typical culture collection center on June 25th, 2008, and preserving number is CCTCC NO:V200806.
By weight, the content of the former powder of described prodenia litura nuclear polyhedrosis virus is 1～10%, and the content of the former powder of emamectin-benzoate is 0.1～3%, and functional aid and solvent supply 100%, and formulation is a suspending agent.
Described functional aid comprises dispersant, stabilizing agent and bright protective agent, and by weight, they are respectively 1～4%, 1～6% and 0.1～1% of composite biological insecticide total amount; Described dispersant is one or more the mixture in lignosulfonates, neopelex, lauryl sodium sulfate, alcohol ethoxylate, alkylphenol ethoxylate, octadecyl sodium sulfosuccinate, the polycarboxylate; Described stabilizing agent is one or more the mixture in ethylene glycol, propane diols, glycerine, diethylene glycol (DEG), triethylene glycol, polyethylene glycol, sucrose, xanthans, the Magnesiumaluminumsilicate; Described bright protective agent is one or more the mixture in vitamin B2, active carbon, starch, the fluorescent whitening agent.
Described solvent is aseptic running water or distilled water.
Prodenia litura NPV-sz-1 bacterial strain provided by the present invention is to gather prodenia litura morbidity larva from suburb, Suzhou crops to separate and get, inoculates by conventional cell culture and live body and breeds, and cell culture and live body inoculation reproduction method are as follows:
1. cell culture
Blastogenesis type virus (buddid virus BV) by prodenia litura NPV-sz-1 infects the spodoptera frugiperda cell of cultivating in the TC-100 medium (Invitrogen company product) (TUAT-Spli221cell).Described TC-100 medium includes 10% hyclone, 100U/ml penicillin and 50U/ml streptomycin, pH6.2,28 ℃ of cultivation temperature.Gather in the crops supernatant after infecting 48h～72h, promptly the BV of prodenia litura NPV-sz-1 is used for viral seed culture of viruses preservation or Quality Identification; Infect 96h～120h and gather in the crops susceptible cell,, be used for viral seed culture of viruses and preserve or Quality Identification through cracking, centrifugal that polyhedron precipitates.
2. live body inoculation reproduction
Spodoptera litura larvae is raised to 5 ages with artificial feed, fully stir with dilution and the artificial feed of prodenia litura NPV-sz-1, add then and eat to larva to be inoculated, room temperature is controlled at 25～28 ℃, and humidity is more than 70%.The death of falling ill successively of the 4th day beginning larva will be about to dead polypide every day and collect from inoculation back, be placed in the insulating box about 10 ℃, allow virus continue amplification in not dead polypide, treat that the dead fully back of larva concentrates purified virus, make former powder.The dilution of described prodenia litura NPV-sz-1 is that the former powder of prodenia litura NPV-sz-1 is diluted to 2 * 10 with sterile water
7PIB/mL.Described artificial feed are the silkworm artificial feed, with the ratio of feed dry powder: sterile water=1:1.8～2.0 feed and water fully mixed and mix thoroughly, and water proof boiling 20min, it is standby to put into refrigerator after the room temperature cooling.
3. toxicity test
(1) LC
50Measure: it is 1.09 * 10 that the former powder of prodenia litura NPV-sz-1 is diluted respectively
3PIB/mL, 1.09 * 10
4PIB/mL, 1.09 * 10
5PIB/mL, 1.09 * 10
64 kinds of concentration such as PIB/mL are got 1～2mL and are added artificial feed respectively, fully kneading in freshness protection package, band after kneading poison artificial feed are twisted into the equal-volume bead, put into the polyester insect box, picking prodenia litura 2 instar larvaes then, 1 in every hole is put into box and is carried out individual feeding.20 larvas in every district are repeated 3 times, put into 26 ℃ of incubators and raise.The result shows, the LC of prodenia litura NPV-sz-1
50Be 1.09 * 10
5PIB/mL.
(2) LT
50Measure: with prodenia litura NPV-sz-1 dilution (1.09 * 10
6PIB/mL) with artificial feed fully kneading in freshness protection package, the malicious artificial feed of the band after the kneading are twisted into the equal-volume bead, put into the polyester insect box, picking prodenia litura 2 instar larvaes then, 1 in every hole is put into box and is carried out individual feeding.20 larvas in every district are repeated 3 times, put into 26 ℃ of incubators and raise.The result shows, the LT of prodenia litura NPV-sz-1
50Be 4～5 days, virulence is obvious.
Compared with prior art; the invention has the advantages that: owing to adopt the emamectin-benzoate and the stronger compound use of prodenia litura NPV-sz-1 of virulence of relative wide spectrum; can utilize the broad spectrum activity of first dimension salt to prevent and treat various pests; also can utilize the high susceptibility of prodenia litura NPV-sz-1 to cause the popular of disease; widen the desinsection scope; improve insecticidal effect; also can delay the generation of pest resistance to insecticide simultaneously; play lasting natural control action; reduce the dosage of chemical pesticide; reduce cost, preserve the ecological environment, have broad application prospects.
Embodiment
Below in conjunction with embodiment the present invention is further described:
Embodiment 1:
With the former powder 100g of 1,000 hundred million PIB/g prodenia litura NPV-sz-1 and content is that 65% the former powder 100g of emamectin-benzoate (first dimension salt) mixes, and adds sterile water 5kg, with the abundant mixing of agitator.Add dispersant neopelex 200g then, stabilizing agent diethylene glycol (DEG) 300g, bright protective agent fluorescent whitening agent 40g is with the abundant mixing of agitator.Be settled to the volume of 10000mL with sterile water, with the abundant mixing of agitator, obtaining content after the packing is 1% first dimension salt-prodenia litura NPV-sz-1 insecticidal suspending agent of 1,000,000,000 PIB/mL.
Dispersant can also be one or more the mixture in lignosulfonates, lauryl sodium sulfate, alcohol ethoxylate, alkylphenol ethoxylate, octadecyl sodium sulfosuccinate, the polycarboxylate.Stabilizing agent also can make one or more the mixture in spent glycol, propane diols, glycerine, triethylene glycol, polyethylene glycol, sucrose, xanthans, the Magnesiumaluminumsilicate.Bright protective agent also can be one or more the mixture in vitamin B2, active carbon, the starch.
Test with first dimension salt-prodenia litura NPV-sz-1 composite biological insecticide that present embodiment provides, the result is as follows:
Test 1: 1% first dimension salt-prodenia litura NPV-sz-1 composite biological insecticide of getting the present embodiment preparation carries out field controling test, controlling object is 1～2 length of time of Spodoptera litura larvae on the green vegetables, per hectare dosage 150g, diluted concentration is 1000 times, simultaneously, first dimension salt and prodenia litura NPV-sz-1 with same concentrations compare, and its result is referring to table 1.As can be seen from Table 1, the correction preventive effect of 4 days and 7 days is 79.22%～89.61% and 89.47%～100% behind 1% first that present embodiment provides dimension salt-prodenia litura NPV-sz-1 composite biological insecticide medicine, and on average preventive effect is 84.42% and 96.05%; Its average preventive effect is respectively than first dimension salt list agent high 2.60% and 3.94%; Than the single agent high 51.95% and 6.58% of prodenia litura NPV-sz-1.
Table 1: composite biological insecticide carries out field controling test
Test 2: 1% first dimension salt-prodenia litura NPV-sz-1 composite biological insecticide of getting present embodiment preparation carries out field controling test, and controlling object is the prodenia litura and 1～2 length of time of diamond-back moth on the little green vegetables, per hectare dosage 150g, 1000 times of diluted concentrations.Simultaneously, compare with the first of same concentrations dimension salt and prodenia litura NPV-sz-1, its result is referring to table 2, table 3.As can be seen from Table 2, the correction preventive effect of 4 days and 7 days is 71.49%～80.43% and 89.71%～95.33% behind 1% first that present embodiment provides dimension salt-prodenia litura NPV-sz-1 composite biological insecticide medicine, and on average preventive effect is 76.58% and 92.95%; Its average preventive effect is respectively than first dimension salt list agent high 5.77% and 4.49%; Than the single agent high 31.64% and 6.81% of prodenia litura NPV-sz-1.As can be seen from Table 3,1% first that present embodiment provides dimension salt-prodenia litura NPV-sz-1 composite biological insecticide to the diamond-back moth medicine after the correction preventive effect of 4 days and 7 days be 84.63%～88.05% and 88.46%～100%, on average preventive effect is 86.58% and 93.50%; Its average preventive effect is respectively than first dimension salt list agent high 2.53% and 4.75%.
Table 2: composite biological insecticide is to the control test of prodenia litura
Table 3: composite biological insecticide is to the control test of diamond-back moth
Test 3: 1% first dimension salt-prodenia litura NPV-sz-1 composite biological insecticide of getting the present embodiment preparation carries out field controling test, and controlling object is 1～2 length of time of melon thin,tough silk snout moth's larva on the cucumber, per hectare dosage 150g, 1000 times of diluted concentrations.Simultaneously, compare with the first of same concentrations dimension salt and clear water, its result is referring to table 4.As can be seen from Table 4, the correction preventive effect of 4 days and 7 days is 84.11%～89.04% and 91.92%～96.69% behind 1% first that present embodiment provides dimension salt-prodenia litura NPV-sz-1 composite biological insecticide, and on average preventive effect is 86.81% and 93.90%; Than first dimension salt list agent high 1.43% and 1.12%, both its average preventive effect remain basically stable respectively.
Table 4: composite biological insecticide is to the control test of melon thin,tough silk snout moth's larva
Above-mentioned indoor bioassay and field controling test result show, the emamectin-benzoate that technical solution of the present invention provides-prodenia litura NPV-sz-1 composite biological insecticide has certain synergistic effect to the control prodenia litura, can obviously alleviate the harm of insect to crops.Simultaneously can doublely control other lepidoptera pests such as diamond-back moth, melon thin,tough silk snout moth's larva.Because two kinds of active ingredients of said preparation are biopesticide, to crops, people and animals and Environmental security, can be used for preventing and treating various lepidoptera pests and the mite class of crops such as vegetables, grain, cotton, fruit tree.
Claims (6)
1. composite biological insecticide is characterized in that its composition is: prodenia litura nuclear polyhedrosis virus content be 500～1,000 hundred million PIB/g the former powder of prodenia litura nuclear polyhedrosis virus, emamectin-benzoate by weight content be 50～95% the former powder of emamectin-benzoate, functional aid and solvent; The production bacterial strain of described prodenia litura nuclear polyhedrosis virus is a prodenia litura nuclear polyhedrosis virus Suzhou strain No.1 (SpltNPV-sz-1), this bacterial strain is deposited in Chinese typical culture collection center on June 25th, 2008, and preserving number is CCTCC NO:V200806; By weight, the content of the former powder of described prodenia litura nuclear polyhedrosis virus is 1～10%, and the content of the former powder of emamectin-benzoate is 0.1～3%, and functional aid and solvent supply 100%, and formulation is a suspending agent.
2. a kind of composite biological insecticide according to claim 1; it is characterized in that: described functional aid comprises dispersant, stabilizing agent and bright protective agent; by weight, they are respectively 1～4%, 1～6% and 0.1～1% of composite biological insecticide total amount.
3. a kind of composite biological insecticide according to claim 2 is characterized in that: described dispersant is one or more the mixture in lignosulfonates, neopelex, lauryl sodium sulfate, alcohol ethoxylate, alkylphenol ethoxylate, octadecyl sodium sulfosuccinate, the polycarboxylate.
4. a kind of composite biological insecticide according to claim 2 is characterized in that: described stabilizing agent is one or more the mixture in ethylene glycol, propane diols, glycerine, diethylene glycol (DEG), triethylene glycol, polyethylene glycol, sucrose, xanthans, the Magnesiumaluminumsilicate.
5. a kind of composite biological insecticide according to claim 2 is characterized in that: described bright protective agent is one or more the mixture in vitamin B2, active carbon, starch, the fluorescent whitening agent.
6. a kind of composite biological insecticide according to claim 1 is characterized in that: described solvent is aseptic running water or distilled water.
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| CN102726453A (en) * | 2012-07-19 | 2012-10-17 | 湖南省烟草公司郴州市公司 | Microbial pesticide for preventing and controlling prodenia litura |
| ES2686748T3 (en) * | 2013-06-21 | 2018-10-19 | Basf Se | Concentrated suspension composition comprising insecticide of isothiazoline and activated carbon |
| CN109912671B (en) * | 2019-04-12 | 2022-04-26 | 宁夏泰益欣生物科技有限公司 | Method for extracting abamectin B2a by using abamectin crystallization mother liquor |
| CN110742091A (en) * | 2019-11-18 | 2020-02-04 | 山东土木启生物科技有限公司 | Compound microbial agent with insect prevention and growth promotion functions |
| CN119082049A (en) * | 2024-10-18 | 2024-12-06 | 湖南省烟草公司郴州市公司 | A kind of Spodoptera litura nuclear polyhedrosis virus SpltMNPV-c5 and its application |
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| CN1650718A (en) * | 2005-02-18 | 2005-08-10 | 李建中 | Insecticide composition containing methyl amino Avermectin and nucleo type polyhedron viruse |
| CN1784977A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院动物研究所 | Insect rhabdovirus insecticide and its prepn. method |
| CN101204161A (en) * | 2007-12-11 | 2008-06-25 | 海南利蒙特生物农药有限公司 | Viral suspension formulation pesticide with emamectin-benzoate |
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| CN1784977A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院动物研究所 | Insect rhabdovirus insecticide and its prepn. method |
| CN1650718A (en) * | 2005-02-18 | 2005-08-10 | 李建中 | Insecticide composition containing methyl amino Avermectin and nucleo type polyhedron viruse |
| CN101204161A (en) * | 2007-12-11 | 2008-06-25 | 海南利蒙特生物农药有限公司 | Viral suspension formulation pesticide with emamectin-benzoate |
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