CN101356906B - Artificial propagation method and apparatus of egg parasitoids - Google Patents

Abstract

The invention discloses an artificial breeding method of egg parasitic wasps and a device thereof. The invention aims at providing an artificial breeding method of the egg parasitic wasps, and mainly aims at providing a new artificial oviposition and breeding method of wasps in a net cage provided with a plurality of layers of grid egg-hanging cards, and providing a device needed by the method. The new artificial oviposition and breeding method is carried out in the net cage provided with a plurality of layers of grid egg-hanging cards, and comprises the following steps: first, a grid hung with the egg card is put on the upper layer of the net cage; after the egg parasitic wasps parasitize, when the egg card is replaced, the grid hung with the egg card is successively moved to the next layer in sequence and in bulk, and then the new grid hung with the egg card is put on the top layer; the egg parasitic wasps are automatically transferred to the new egg card; after oviposition, each layer of the grid egg-hanging card is sequentially moved down to one layer and the new grid egg-hanging card is put on the top layer; finally, the egg cards are removed from the net cage in bulk. The new artificial oviposition and breeding method has the advantages of avoiding the procedures of artificial slapping or sweeping, avoiding the damage to mother wasps in the procedure, and realizing the replacement of the egg card without damaging the mother wasps.

Description

Method and device for artificial reproduction of egg parasitoids

Technical field:

The invention belongs to biological technology, and relates to an artificial propagation method of egg parasitoids and a device required for applying the method.

Background technique:

Egg parasitoids are important natural enemy insects for the biological control of agricultural and forestry pests. They lay their eggs in the eggs of parasitic insects, and the larvae feed on the nutrients in the parasitic eggs, thereby achieving the purpose of eliminating natural enemy insects. Ovum parasitoids can distinguish between hosts that have been parasitized and healthy hosts, and avoid over-parasitism or multi-parasitism. Therefore, the same species of oviparasitoids generally do not lay eggs again in parasitized eggs. The flat-bellied wasp is a natural enemy insect for the biological control of litter bugs and pine caterpillars, and it often breeds this bee from November to March of the next year. During this period, due to the low temperature in South China, it is necessary to breed in the laboratory under relatively stable temperature and humidity conditions. At present, glass bottles (tubes) or plastic bottles (tubes, boxes) are used for a small number of bees; a large number of bees are carried out by hanging egg cards on brackets between numerous bees or hanging egg cards on darkroom curtain walls. The flat-bellied wasp has a long egg-laying period and can continuously lay eggs for more than 10 days. When breeding this bee, the host egg card needs to be replaced every day or every other day to make full use of the reproductive efficiency of the adult bee. Because the flat-bellied wasp is parasitizing or looking for host eggs During the process, it has a strong chemotaxis to the host eggs, and is often tightly attached to the egg card and is not easy to separate. When the egg card is replaced, it is easy to damage the adult bees by beating them, which will affect the lifespan of the adult bees and reduce the efficiency of breeding bees. Glass bottles (tubes) or plastic bottles (tubes, boxes) have low breeding efficiency and fail to meet the needs of large-scale applications; the two methods used by a large number of bees cannot solve the problem of flat-bellied bees escaping from light and beating. Egg card causes adult bee damage and causes the difficult problem that kind of bee wastes.

Invention content:

The present invention proposes an improved method for the artificial reproduction of egg parasitoids, which mainly lies in proposing a new method for artificially laying eggs and breeding in a cage that can be provided with multi-layer grid hanging egg cards, and provides a method for applying the method. The required egg parasitoid artificial breeding net cage is used to overcome the deficiencies of the prior art, thereby improving the breeding efficiency, and to achieve the production line of numerous bees.

To achieve the above object, the present invention proposes the following technical solutions:

During the artificial breeding process of egg parasitoids, a new method for artificially laying eggs and breeding in a net cage that can be provided with multi-layer grids to hang egg cards, the steps are as follows:

(1) Put the egg parasitoid artificial breeding net cage into the breeding room that can control light and temperature and humidity, open the box door, put the grid with the egg card into the upper layer of the box, insert the egg parasitoid, let Egg parasitoids lay eggs on the host egg card;

(2) After the egg-parasitic wasp lays eggs on the egg card, the whole batch of the egg-hanging cards on the upper grid is transferred to the next layer at one time, and the grid with the new egg card is placed on the upper layer. Bees have the characteristics of phototaxis, chemotaxis, and upwards. Generally, the same egg parasitoid will not lay eggs again in the eggs that have been parasitized, but will transfer to fresh eggs to lay eggs. It uses the flying and crawling abilities of egg parasitoids. , let it transfer to a new host egg card to lay eggs by itself;

(3) After the egg parasitoid lays eggs on the new host egg card, the above-mentioned layers of grid hanging egg cards are moved down one layer in turn, and then the grid with the new egg card is placed on the upper layer, so that it Self-transfer to a new host egg card to lay eggs;

(4) When there is no female wasp parasitizing the egg card in the lower layer, the whole layer of grid hanging egg card is removed from the cage;

The number of cycles of the above-mentioned process depends on the number of layers of grid hanging egg cards in the net cage;

The egg cards removed from the net cage are placed under conventional culture conditions to allow the parasitic eggs to develop to the pupal stage and then refrigerated as a bee source for female bees or commercial bees.

In order to realize the above-mentioned method, the present invention also provides a cage for the artificial propagation of egg parasitoids:

The artificial breeding cage of the egg parasitoid is provided with a support, and several layers of grids on which egg cards can be hung are arranged on the support. The distance between the grids is 20-40 cm, and there is a movement The loading and unloading structure has a door on one side of the cage.

The grid and the support are connected by a hook-type mobile loading and unloading structure, the grid is rectangular, 2 to 5 are arranged, and there are 15 to 45 horizontal bars on which egg cards can be hung.

The net cage is composed of a stainless steel skeleton with a 100-160 mesh gauze.

Described door is that the gauze on the front side of the net cage is connected with the net cage by a slide fastener to form a door, so as to operate when placing, taking out and replacing the egg card, and also to facilitate replacement and cleaning.

The stainless steel skeletons can be connected by ferrules, which are convenient for installation and disassembly, and are conducive to long-distance transportation. In non-breeding bee seasons, they can be disassembled and packed in packing boxes, occupying a small area and being flexible and convenient to use.

The beneficial effects of the present invention are as follows:

1. Adopt the new method of artificial spawning and reproduction in the multi-layer grid cage with egg cards. When replacing the egg cards, you only need to move the grids with egg cards to the next layer one by one. Then put the new grid with the egg card on the top layer, and the mother bee will transfer to the new egg card to lay eggs, eliminating the manual beating or sweeping process, and avoiding the damage to the mother bee during this process. The longevity of the mother bee and the reduction of the efficiency of the breeding bee have realized the process of replacing the egg card without harming the mother bee.

2. Use the flying and crawling of the female bee to transfer to the new egg card by itself, which improves the vigor of the offspring of the egg parasitoid bee and ensures the quality of the commercial bee.

3. The net cage is a ventilated and light-transmitting airtight space, which is beneficial to prevent the escape of parasitoid eggs by phototaxis.

Description of drawings:

Fig. 1 is the schematic diagram of flat-bellied wasp artificial breeding cage;

Detailed ways:

The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

As shown in the accompanying drawing, the net cage for the artificial propagation of the flat-bellied wasp provided by the present invention is arranged as a cuboid, and the size can be set as required. The length, width and height set in this embodiment are respectively 100cm, 40cm and 100cm. The skeleton coat 120 mesh nylon gauze constitutes the whole cage, and the cage is provided with a bracket 4 on which a three-layer rectangular grid 1 can be placed. It can be removed from the bracket 4. The grid 1 is placed horizontally. The distance between the grids is 30cm. There are 30 cross bars 2 on the grid 1. The clips hold the egg card and hang on the cross bar 2. On each cross bar 2, two egg cards can be hung, and the length and width of the egg card are respectively set to 28cm and 18cm. , When taking out and replacing the egg card, it is also easy to replace and clean. The stainless steel frame is connected by a ferrule-type connection, which is easy to install and disassemble, and is conducive to long-distance transportation. Box, small footprint, flexible and convenient to use.

In the process of artificial propagation of the flat-bellied wasp, the artificial breeding cage of the above-mentioned flat-bellied wasp is utilized to realize a new method of artificially laying eggs and breeding in the cage that can be provided with a multi-layer grid hanging egg card, and its steps are as follows:

1. Put the artificial breeding cage of the flat-bellied bee into the breeding room that can control the light and temperature and humidity, open the door of the box, put the grid with the egg card on the upper layer of the box, and connect the flat-bellied wasp, Let the flat-bellied wasp lay eggs on the host egg card;

2. When the flat-bellied wasp lays eggs on the egg card, when the egg card is replaced regularly (generally takes 1 to 2 days), the grid with the egg card that has been parasitized is adjusted to the middle layer, and the grid with the new egg card is hung. The grid of the egg card is placed on the upper layer, allowing it to automatically transfer to a new host egg card to lay eggs;

3. After the flat-bellied wasp lays eggs on the new host egg card, when the egg card is replaced regularly (generally takes 1 to 2 days), the above two layers of grid hanging egg cards are moved down one layer in turn, and the The grid with the new egg card hanging on it is placed on the upper layer, allowing it to transfer to the new host egg card to lay eggs by itself;

4. When there is no female bee parasitizing the egg card in the lower layer, remove the egg card on the grid of the layer out of the cage as a whole;

If there is also female honeybee in the net cage and can continue to circulate steps 2～4, if not then can all move out the grid that is hung with ovum card.

The egg card removed from the net cage is placed under conventional culture conditions to allow the parasitic eggs to develop to the pupal stage and then refrigerated, as a bee source for female bees or commercial bees.

2 egg cards can be hung on each horizontal bar of the grid described in the present invention, and 60 egg cards can be hung on each layer. The number of host eggs of each egg card is about 3000 tussah silkworm eggs or 50000 rice moth eggs. When the number of female bees is sufficient, the bee card can be replaced once a day, and 150,000 to 160,000 flat-bellied wasps are bred (according to about 85% parasitic rate).

Claims (9)

1. egg parasitoids artificial fecundation method is characterized in that:

(1) carry out the artificial spawning breeding in the net cage that multilayer grid extension ovum card can be set, step is as follows:

(1) grid that will hang with the ovum card places the upper strata, allows the egg parasitoids parasitism;

(2) when egg parasitoids after laying eggs on the ovum card, this upper strata grid hung the ovum card is disposable by the gross is transferred to down one deck, and the new grid that hangs with the ovum card placed the upper strata, allow egg parasitoids transfer to voluntarily and lay eggs on the new host egg card;

(3) when egg parasitoids after laying eggs on the new ovum card, above-mentioned each layer grid extension ovum card is moved down one deck successively, and the newer grid that hangs with the ovum card is placed the upper strata, allow egg parasitoids transfer to voluntarily again and lay eggs on the new host egg card;

(4) when not having queen bee parasitic on the grid extension ovum card of lower floor, this layer grid hung ovum card integral body shift out net cage;

The cycle-index of said process is hung the ovum card number of plies according to the grid in the net cage and is decided;

(2) will shift out the ovum card of net cage, and place to allow egg parasitoid grow to the pupa time refrigeration under the conventional condition of culture as the honeybee source of queen bee or commodity honeybee.

2. egg parasitoids artificial propagation net cage that is applied to the described method of claim 1, it is characterized in that having support in the net cage, support is provided with the grid that several layers can hang the ovum card, distance between grid and the grid is 20～40cm, the structure that has the loading and unloading of moving between grid and the support is provided with door in a side of net cage.

3. egg parasitoids artificial propagation net cage according to claim 2 is characterized in that adopting between described grid and the support coupling type to move construct for handling.

4. egg parasitoids artificial propagation net cage according to claim 2 is characterized in that described grid is a rectangle.

5. egg parasitoids artificial propagation net cage according to claim 2 is characterized in that described grid is set to 2～5.

6. according to claim 2,3,4 or 5 described egg parasitoids artificial propagation net cages, it is characterized in that described grid has 15～45 cross bars that can hang the ovum card.

7. egg parasitoids artificial propagation net cage according to claim 2 is characterized in that described net cage is made of skeleton outer cover gauze.

8. egg parasitoids artificial propagation net cage according to claim 7 is characterized in that described Net-cage frame is a stainless steel insert.

9. egg parasitoids artificial propagation net cage according to claim 7, the aperture that it is characterized in that described gauze is 100～160 orders.

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