CN101329328A - Oxalic acid diagnosis / determination reagent kit and method for measuring oxalic acid concentration - Google Patents
Oxalic acid diagnosis / determination reagent kit and method for measuring oxalic acid concentration Download PDFInfo
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- CN101329328A CN101329328A CNA2007100247199A CN200710024719A CN101329328A CN 101329328 A CN101329328 A CN 101329328A CN A2007100247199 A CNA2007100247199 A CN A2007100247199A CN 200710024719 A CN200710024719 A CN 200710024719A CN 101329328 A CN101329328 A CN 101329328A
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- CN
- China
- Prior art keywords
- reagent
- oxalic acid
- coenzyme
- stabilizing agent
- oxalate oxidase
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- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 title claims abstract description 131
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 61
- 235000006408 oxalic acid Nutrition 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000003745 diagnosis Methods 0.000 title claims abstract description 15
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims abstract description 40
- 239000005515 coenzyme Substances 0.000 claims abstract description 28
- 239000003381 stabilizer Substances 0.000 claims abstract description 25
- 101710088194 Dehydrogenase Proteins 0.000 claims abstract description 23
- 108010063734 Oxalate oxidase Proteins 0.000 claims abstract description 22
- 229940100228 acetyl coenzyme a Drugs 0.000 claims abstract description 20
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract 2
- 102000004190 Enzymes Human genes 0.000 claims abstract 2
- 238000013016 damping Methods 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 238000002835 absorbance Methods 0.000 claims description 10
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 4
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229940107700 pyruvic acid Drugs 0.000 claims description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 5
- 235000011187 glycerol Nutrition 0.000 claims 2
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 abstract description 2
- 238000004737 colorimetric analysis Methods 0.000 abstract description 2
- 238000006911 enzymatic reaction Methods 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000007853 buffer solution Substances 0.000 abstract 1
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 2
- 241000218993 Begonia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001991 dicarboxylic acids Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229940076788 pyruvate Drugs 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 150000002910 rare earth metals Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to an oxalic acid diagnosis/determination kit which uses an enzyme colorimetry technique; simultaneously, the invention also relates to a method principle used for determining oxalic acid consistency, reagent composition and components, belonging to the field of food detection determination technique. The main compositions of the kit of the invention comprise buffer solution, reduction-typed coenzyme, acetyl coenzyme A, oxalate oxidase, pyruvate dehydrogenase and stabilizer; samples and reagents are mixed according to a certain volume proportion so as to generate a series of enzymatic reactions; subsequently, reaction matters are arranged under an ultraviolet/visible light analyser so as to detect the degree/speed of the absorbency reduction of the main wavelength at the 340nm position, thus calculating the consistency of the oxalic acid.
Description
Technical field
The present invention relates to a kind of oxalic acid diagnosis/mensuration kit, the invention still further relates to the method for measuring concentration of oxalic acid simultaneously, belong to medical science/Food Inspection determination techniques field.
Background technology
Oxalic acid is prevalent in the plantage as the simplest a kind of dicarboxylic acids, and plant oxalic acid has the important physical function, and plays a positive role in stress resistance of plant.The many forms with sylvite or calcium salt of oxalic acid exist, and the form with free acid exists in begonia, bajiao banana, and oxalic acid is poisonous, can be dissolved in water.Oxalic acid is a kind of important chemical material, is widely used in the separation of medicine, dyestuff, coating, rare earth metal and the bleaching of purification and clothing.
The method of measuring oxalic acid has: the chromatography of ions, the sub-chromatography of electric diversion, HPLC method.Most of clinically calculus are drafted a document sour calcium calculus.
Summary of the invention
The technical problem to be solved in the present invention is: propose a kind of enzymic colorimetric (EnzymaticColorimetric Method) and coupling method (Couple Reaction) technology utilized, monitoring reduced form nicotinamide coenzyme (reduced coenzyme) is in the variation of 340nm wavelength place absorbance, measured the method for concentration of oxalic acid, simultaneously, the present invention also will provide in order to the oxalic acid diagnosis of realizing this method/mensuration kit, adopt this reagent not only can be ultraviolet analyser or half, carrying out concentration of oxalic acid on the automatic clinical chemistry analyzer measures, and finding speed is fast, the accuracy height, thereby can obtain practical applying.
Concentration of oxalic acid assay method principle of the present invention is as follows:
Oxalic acid+oxygen
Oxalate oxidaseCarbon dioxide+hydrogen peroxide
Carbon dioxide+acetyl coenzyme A+reduced coenzyme
Pyruvic dehydrogenase
Pyruvic acid+coacetylase+coenzyme
This method is used oxalate oxidase (oxalate oxidase; EC 1.2.3.4) coupling pyruvic dehydrogenase (pyruvate dehydrogenase; EC 1.2.1.51) enzymatic reaction continuous monitoring method/terminal colorimetric analysis.The reaction of oxalate oxidase enzymolysis oxalic acid produces carbon dioxide, effect by the coupling pyruvic dehydrogenase again, reduced coenzyme (absorption peak being arranged at the 340nm place) is oxidized into coenzyme (not having absorption peak at the 340nm place) the most at last, thereby measured degree/speed that reduced coenzyme descends in 340nm place absorbance, by measuring degree/speed that 340nm place absorbance descends, can calculate the concentration of oxalic acid.
Experiment shows, takes all factors into consideration from the accuracy of measurement result and economy two aspects of preparation cost, no matter is single agent, two agent or three doses, and oxalic acid diagnosis of the present invention/the mensurations kit of following composition relation is ideal comparatively:
Damping fluid 100mmol/L
Stabilizing agent 500mmol/L
Reduced coenzyme 0.25mmol/L
Oxalate oxidase 10000U/L
Pyruvic dehydrogenase 12000U/L
Acetyl coenzyme A 20mmol/L
Oxalic acid diagnosis of the present invention/mensuration kit can be single agent, comprising:
Damping fluid, stabilizing agent, reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, acetyl coenzyme A.
Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Also above-mentioned single agent reagent can be made into following pair of agent reagent:
Reagent 1
Damping fluid, stabilizing agent, reduced coenzyme, acetyl coenzyme A.
Reagent 2
Damping fluid, stabilizing agent, oxalate oxidase, pyruvic dehydrogenase.
Reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, the position of acetyl coenzyme A in reagent 1 or reagent 2 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Above-mentioned single agent reagent can also be made into following three doses of reagent:
Reagent 1
Damping fluid, stabilizing agent, reduced coenzyme, acetyl coenzyme A.
Reagent 2
Damping fluid, stabilizing agent, pyruvic dehydrogenase.
Reagent 3
Damping fluid, stabilizing agent, oxalate oxidase.
Reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, the position of acetyl coenzyme A in reagent 1, reagent 2 or reagent 3 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
No matter be single agent, two agent or three doses, the present invention measures the method for concentration of oxalic acid, and its reduced coenzyme can be a kind of among NADPH, NADH or the thio-NADH.
Embodiment
The present invention is further illustrated below in conjunction with examples of implementation.
Embodiment one
The oxalic acid diagnosis of present embodiment/mensuration reagent is single reagent, comprising:
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Reduced coenzyme 0.25mmol/L
Oxalate oxidase 10000U/L
Pyruvic dehydrogenase 12000U/L
Acetyl coenzyme A 20mmol/L
Reagent divides the bottle of packing into after all dissolving and preparing, and carries out freeze drying, makes powdered reagent; Before the use, add pure water, use after redissolving.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance 1.8 ± 0.7, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested oxalic acid sample and reagent is 1/25, the Direction of Reaction is negative reaction (reaction descends), about about 0 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance descends, thereby calculates the concentration of oxalic acid.
Embodiment two
The oxalic acid diagnosis of present embodiment/mensuration reagent is double reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Reduced coenzyme 0.25mmol/L
Acetyl coenzyme A 20mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Oxalate oxidase 10000U/L
Pyruvic dehydrogenase 12000U/L
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid double reagent, can directly use.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance 1.8 ± 0.7, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested oxalic acid sample and reagent 1, reagent 2 is 2/20/5, the Direction of Reaction is negative reaction (reaction descends), about about 0 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance descends, thereby calculates the concentration of oxalic acid.
Embodiment three
The oxalic acid diagnosis of present embodiment/mensuration reagent is three reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Reduced coenzyme 0.25mmol/L
Acetyl coenzyme A 20mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Pyruvic dehydrogenase 12000U/L
Reagent 3
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Oxalate oxidase 10000U/L
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid three reagent, can directly use.
When measuring concentration of oxalic acid, on automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance 1.8 ± 0.7, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested oxalic acid sample and reagent 1, reagent 2, reagent 3 is 4/40/5/5, the Direction of Reaction is negative reaction (reaction descends), and about about 0 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance descends, thereby calculates the concentration of oxalic acid.
The applicant adopts other various reduced form chromogens combinations of putting down in writing in the above summary of the invention all can reach purpose of the present invention through experimental verification, in view of situation such as determination step and above embodiment roughly the same, do not separately enumerate.
In a word, experiment showed, and adopt assay method of the present invention can draw required measurement result by general biochemical analyzer fully, and highly sensitive, degree of accuracy good, and is easy to utilize.
Claims (6)
1. concentration of oxalic acid assay method that utilizes enzymic colorimetric and enzyme-linked method technology, its method principle is as follows:
Oxalic acid+oxygen
Oxalate oxidaseCarbon dioxide+hydrogen peroxide
Carbon dioxide+acetyl coenzyme A+reduced coenzyme
Pyruvic dehydrogenase
Pyruvic acid+coacetylase+coenzyme
The end reaction thing is placed under ultraviolet analyser or half, the automatic clinical chemistry analyzer, detect degree/speed that predominant wavelength 340nm absorbance descends, calculate the concentration measurement result of oxalic acid.
2. oxalic acid diagnosis/mensuration kit, principal ingredient comprises:
Damping fluid 20-500mmol/L
Stabilizing agent 1-4000mmol/L
Reduced coenzyme 0.1-0.35mmol/L
Oxalate oxidase 1000-80000U/L
Pyruvic dehydrogenase 1000-80000U/L
Acetyl coenzyme A 1-50mmol/L
It is characterized in that: kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
3. according to the described oxalic acid diagnosis of claim 2/mensuration kit, it is characterized in that:
Form single agent reagent by damping fluid, stabilizing agent, reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, acetyl coenzyme A.
4. according to the described oxalic acid diagnosis of claim 2/mensuration kit, it is characterized in that:
Form two agent reagent by damping fluid, stabilizing agent, reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, acetyl coenzyme A; Reagent 1 is made up of damping fluid, stabilizing agent, reduced coenzyme, acetyl coenzyme A; Reagent 2 is made up of damping fluid, stabilizing agent, oxalate oxidase, pyruvic dehydrogenase.Reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, the position of acetyl coenzyme A in reagent 1 or reagent 2 can not limit.
5. according to the described oxalic acid diagnosis of claim 2/mensuration kit, it is characterized in that: form multi-agent reagent by damping fluid, stabilizing agent, reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, acetyl coenzyme A; Reagent 1 is made up of damping fluid, stabilizing agent, reduced coenzyme, acetyl coenzyme A; Reagent 2 is made up of damping fluid, stabilizing agent, pyruvic dehydrogenase; Reagent 3 is made up of damping fluid, stabilizing agent, oxalate oxidase.Reduced coenzyme, oxalate oxidase, pyruvic dehydrogenase, the position of acetyl coenzyme A in reagent 1, reagent 2 or reagent 3 can not limit.
6. according to the described oxalic acid diagnosis of claim 2/mensuration kit, it is characterized in that: also comprise stabilizing agent 1-4000mmol/L or 0.1%-100% volume ratio.Described stabilizing agent is: ammonium sulfate (Ammonia Sulfate), glycerine (Glycerol), propylene glycol (Propylene Glycol), ethylene glycol (Ethylene glycol) and at least one of the preservatives.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100247199A CN101329328A (en) | 2007-06-21 | 2007-06-21 | Oxalic acid diagnosis / determination reagent kit and method for measuring oxalic acid concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100247199A CN101329328A (en) | 2007-06-21 | 2007-06-21 | Oxalic acid diagnosis / determination reagent kit and method for measuring oxalic acid concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101329328A true CN101329328A (en) | 2008-12-24 |
Family
ID=40205241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100247199A Pending CN101329328A (en) | 2007-06-21 | 2007-06-21 | Oxalic acid diagnosis / determination reagent kit and method for measuring oxalic acid concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101329328A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104406949A (en) * | 2014-12-02 | 2015-03-11 | 武汉瑞恒达生物工程有限公司 | Reagent, kit and method for detecting content of oxalic acid in urine and blood |
| CN104677959A (en) * | 2015-03-17 | 2015-06-03 | 武汉康复得生物科技股份有限公司 | Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper |
| CN110699423A (en) * | 2019-10-22 | 2020-01-17 | 浙江大学 | A kind of reagent, kit and method for detecting oxalic acid concentration |
-
2007
- 2007-06-21 CN CNA2007100247199A patent/CN101329328A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104406949A (en) * | 2014-12-02 | 2015-03-11 | 武汉瑞恒达生物工程有限公司 | Reagent, kit and method for detecting content of oxalic acid in urine and blood |
| CN104677959A (en) * | 2015-03-17 | 2015-06-03 | 武汉康复得生物科技股份有限公司 | Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper |
| CN110699423A (en) * | 2019-10-22 | 2020-01-17 | 浙江大学 | A kind of reagent, kit and method for detecting oxalic acid concentration |
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Open date: 20081224 |