CN101278041A - Genes from actinobacillus succinogenes 13oz (atcc 55618) for production of chemicals from the a. succinogenes C4-pathway - Google Patents

Abstract

The present invention discloses Actinobacillus succinogenes genes and methods of utilizing these genes in genetically engineered A. - Ketoglutaric acid, glutamic acid, and aspartic acid. The genetically engineered strain of A. succinogenes is capable of overexpressing the C4 enzyme. The genetically engineered A. succinogenes may have one or more gene knockouts or modifications that inhibit C3 enzymes. In some genetically engineered A. succinogenes, it is also possible to increase the flux supplying C4 pathway substrates.

Description

Genes from ACTINOBACILLUS SUCCINOGENES130Z (ATCC 55618) for the production of chemical products from the A. SUCCINOGENES C4-pathway

Cross References to Related Applications

[0001] This application claims the benefit of Provisional Application No. 60/705,841, filed August 5, 2005, the entire contents of which are incorporated herein by reference.

Statement Regarding Federally Funded Research or Development

[0002] This work was supported by funding from the National Science Foundation. It was made possible by the genome sequencing efforts of the Department of Energy's Joint Genome Institute. The US Government has certain rights in this invention.

Background of the invention

a.Technical field

[0003] The present invention relates generally to metabolic engineering, and more particularly to engineering Actinobacillus succinogenes to produce Chemicals such as succinate, fumarate, malate, 5-aminoacetyl 5-aminolevulinate, 2-oxoglutarate, glutamate, and aspartate. In particular, the invention relates to inhibiting C3 pathway enzymes and overexpressing C4 pathway enzymes. The present invention also relates to increasing the flux of substrate supply to the C4 pathway. The invention also relates to increasing the rate of secretion of products from the C4 pathway.

b. Description of related technologies

[0004] Succinic acid has many industrial fine chemical applications. Succinic acid can also be used as an intermediate commodity chemical feedstock for the production of bulk chemicals. Its greatest market potential may lie in its use as a feedstock for the production of plastics stronger than steel, biodegradable chelating agents, and green solvents. Most of the 17,000 tons (15,400 metric tons) of succinic acid sold annually are produced petrochemically from maleic acid. Succinic acid is also produced as a fine chemical product by the fermentation of glucose. In order for fermentation to be competitive in the production of succinic acid as a commodity chemical product, the overall production cost should be reduced from one (1) dollar per pound ($2.20/kg) to about twenty (20) cents per pound (44 cents /kg).

[0005] U.S. Patent No. 5,143,833 to Datta et al. teaches a method of producing succinic acid by culturing a succinic acid-producing microorganism Anaerobiospirillum succiniciproducens under specific conditions.

[0006] U.S. Reissued Patent No. RE37,393 to Donnelly et al. teaches a method for isolating succinic acid-producing bacteria by increasing the Biomass, which was then grown in glucose-rich media under anaerobic conditions to enable growth of mutants that catabolize pyruvate. In this way, Donnelly provided mutant E. coli capable of producing large quantities of succinate. The mutant Escherichia coli was derived from a parent lacking the genes for pyruvate formate lyase and lactate dehydrogenase.

[0007] U.S. Patent No. 6,455,284 and U.S. Patent Application Publication No. 2003/0087381, both to Gokarn et al., teach metabolic engineering to increase carbon flow towards oxaloacetate to increase bioactivity in bacterial and industrial fermentation. production of biochemicals such as lysine and succinic acid. This carbon flow was redirected by genetically engineering bacteria to overexpress the enzyme pyruvate carboxylase.

[0008] U.S. Patent Application Publication No. 2003/0113885 to Lee et al. teaches a novel microorganism Mannheimia sp.55E capable of producing organic acids, and uses said microorganism to produce organic acids by anoxic and aerobic cultivation. sour method.

[0009] US Patent No. 6,420,151 to Eikmanns et al. teaches an isolated nucleic acid from coryneform bacteria encoding phosphoenolpyruvate carboxykinase, which is involved in the production of succinate.

[0010] U.S. Patent Nos. 5,504,004 and 5,723,322 to Guettler et al. teach a method for the preparation of succinic acid, a novel microorganism Actinobacillus succinogenes 130Z for use in said method, and methods of obtaining said microorganism.

[0011] US Patent No. 5,573,931 to Guettler et al. teaches methods for preparing succinic acid, A. succinogenes variants for use in the methods, and methods for obtaining the variants.

[0012] Although the methods described above can be used to produce succinic acid, Actinobacillus succinogenes is still the best known producer of succinic acid. A. succinogenes are Gram-negative capnophilic, anaerobic bacteria belonging to the Pasteurellaceae. Under optimal conditions A. succinogenes produces up to one hundred (100) grams/liter of succinic acid. Much effort has been devoted to engineering E. coli strains to produce succinate in large quantities, but none of the engineered E. coli strains surpassed A. succinogenes in succinate production. Carbon flux is tightly regulated in microbial metabolism, including carbon flux towards oxaloacetate. Overcoming this control of carbon flux would potentially increase the yield of desired products such as succinic acid, fumaric acid, malic acid, 5-aminolevulinic acid, 2-ketoglutaric acid, and glutamic acid.

[0013] Although the related art teaches the production of succinic acid, there is still a need for improved production chemicals (such as succinic acid, fumaric acid, malic acid, 5-aminolevulinic acid, 2-ketoglutaric acid, glutamic acid, and aspartate) by inhibiting C3 pathway enzymes and overexpressing C4 pathway enzymes, and also by increasing the flux of supply substrate to the C4 pathway and increasing the rate of secretion of the product.

purpose of invention

[0014] Accordingly, it is an object of the present invention to provide gene sequences that can be overexpressed, knocked out, or altered to benefit the production of chemical products.

[0015] Another object of the present invention is to provide a method for engineering A.succinogenes to produce chemical products such as succinic acid, fumaric acid, malic acid, 5-aminolevulinic acid, 2-ketoglutarate, glutamate, and aspartate.

[0016] These and other objects will become apparent by reference to the following description.

Contents of the invention

[0017] The present invention provides a method for producing succinic acid comprising: providing genetically engineered A. succinogenes comprising one or more gene knockouts or modifications inhibiting C3 enzymes selected from the group consisting of pyruvate Kinase, pyruvate-formate lyase (pyruvate-formatelyase), pyruvate dehydrogenase, and the group consisting of combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and in said The genetically engineered A. succinogenes were cultured in growth medium to produce succinate.

[0018] The present invention provides a method for producing succinic acid comprising: providing genetically engineered A. succinogenes capable of overexpressing a C4 enzyme selected from the group consisting of phosphoenolpyruvate carboxykinase (PEPCK), The group consisting of malate dehydrogenase, fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and culturing said gene in said growth medium Engineering of A. succinogenes to produce succinate.

[0019] The invention provides a method for producing succinic acid, comprising: providing genetically engineered A.succinogenes comprising one or more gene knockouts or modifications inhibiting C3 enzymes and capable of overexpressing C4 enzymes, so The C3 enzyme is selected from the group consisting of pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, and the C4 enzyme is selected from the group consisting of PEPCK, malate dehydrogenase, fumarase, fumarate reductase , and the group consisting of combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium to produce succinic acid. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications to one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase formed group. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase flux through the Embden-Meyerhof-Parnas (EMP) pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase flux through the pentose phosphate pathway (PPP). In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes to increase substrate uptake. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes to increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose . In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance chemical excretion. In additional embodiments, the one or more genes encode dicarboxylate transporters.

[0020] The present invention provides a method for producing fumaric acid, comprising: providing genetically engineered A. succinogenes comprising one or more knockout or modified genes inhibiting C3 enzymes selected from pyruvate kinase , pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium Synthesis of A. succinogenes to produce fumaric acid.

The invention provides the method for producing fumaric acid, comprising: providing the A.succinogenes of the genetic engineering that can overexpress C4 enzyme, described C4 enzyme is selected from PEPCK, malate dehydrogenase, fumarase and combination thereof; providing a growth medium for culturing the genetically engineered A. succinogenes; and culturing the genetically engineered A. succinogenes in the growth medium to produce fumaric acid.

The present invention provides the method for producing fumaric acid, comprising: A.succinogenes of genetic engineering is provided, and it comprises one or more gene knockouts that suppress C3 enzyme or modification and can overexpress C4 enzyme, described C3 enzyme selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, the C4 enzyme being selected from PEPCK, malate dehydrogenase, fumarase, and combinations thereof; providing for culturing the A growth medium of the genetically engineered A. succinogenes; and culturing the genetically engineered A. succinogenes in the growth medium to produce fumaric acid. In additional embodiments, the genetically engineered A. succinogenes further comprises modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit fumarate reductase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the (EMP) pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications to one or more genes that improve passage through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter.

[0023] The present invention provides a method for producing malic acid, comprising: providing genetically engineered A. succinogenes comprising one or more knockout or modified genes inhibiting C3 enzymes selected from the group consisting of pyruvate kinase, Pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; cultivating said genetically engineered A. succinogenes in said growth medium A. succinogenes to produce malic acid.

The present invention provides the method for producing malic acid, comprising: providing the A.succinogenes of the genetic engineering that can overexpress C4 enzyme, described C4 enzyme is selected from PEPCK, malate dehydrogenase, and combination thereof; a growth medium for culturing said genetically engineered A. succinogenes; and culturing said genetically engineered A. succinogenes in said growth medium to produce malic acid.

[0025] The present invention provides a method for producing malic acid, comprising: providing genetically engineered A.succinogenes comprising one or more gene knockouts or modifications that inhibit C3 enzymes and capable of overexpressing C4 enzymes, said C3 Enzymes selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, said C4 enzymes selected from PEPCK, malate dehydrogenase, and combinations thereof; providing for culturing said gene A growth medium for engineered A. succinogenes; and culturing said genetically engineered A. succinogenes in said growth medium to produce malic acid.

In another embodiment, the A.succinogenes of described genetic engineering further comprises the modification of one or more genes that can bridge the C4 and C3 pathways, and the genes are selected from the group consisting of malic enzyme and oxaloacetate decarboxylation enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit fumarate reductase. 22. The method of claim 22, 23, or 24, wherein said genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit fumarate reductase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter.

The present invention provides a method for producing 5-aminolevulinic acid: comprising: providing genetically engineered A.succinogenes comprising one or more gene knockouts or modifications that inhibit C3 enzymes, said C3 enzymes being selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; culturing said growth medium in said growth medium Genetically engineered A. succinogenes to produce 5-aminolevulinic acid.

The present invention provides the method for producing 5-aminolevulinic acid, comprising: providing the A.succinogenes of genetic engineering that can overexpress C4 enzyme, described C4 enzyme is selected from succinyl-CoA synthetase, PEPCK, apple Acid dehydrogenase, fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium .succinogenes to produce 5-aminolevulinic acid.

The present invention provides the method for producing 5-aminolevulinic acid: comprise: provide the A.succinogenes of genetic engineering, it comprises one or more gene knockouts that suppress C3 enzyme or modify and can overexpress C4 enzyme , the C3 enzyme is selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, and the C4 enzyme is selected from succinyl-CoA synthetase, PEPCK, malate dehydrogenase, Fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium to produce 5 -Aminolevulinic acid. In additional embodiments, the genetically engineered A. succinogenes further comprises modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing an enzyme leading to glycine synthesis. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing 5-aminolevulinate synthase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter. In additional embodiments, the genetically engineered A. succinogenes further comprise modification of one or more glycine synthesis genes.

[0030] The present invention provides a method of producing 2-oxoglutarate comprising: providing genetically engineered A. succinogenes comprising one or more gene knockouts or modifications inhibiting a C3 enzyme selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; culturing said growth medium in said growth medium Genetically engineered A. succinogenes to produce 2-oxoglutarate.

[0031] The present invention provides a method of producing 2-oxoglutarate comprising: providing genetically engineered A. succinogenes capable of overexpressing a C4 enzyme selected from the group consisting of succinyl-CoA synthesis Enzymes, PEPCK, malate dehydrogenase, fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and culturing said growth medium in said growth medium Genetically engineered A. succinogenes to produce 2-oxoglutarate.

[0032] The present invention provides a method for producing 2-oxoglutarate comprising: providing genetically engineered A. succinogenes comprising one or more gene knockouts or modifications that inhibit C3 enzymes and are capable of overexpressing C4 enzymes , the C3 enzyme is selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, and the C4 enzyme is selected from succinyl-CoA synthetase, PEPCK, malate dehydrogenase, fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and culturing said genetically engineered A. succinogenes in said growth medium to produce 2 - Ketoglutarate.

[0033] In additional embodiments, the genetically engineered A.succinogenes further comprise gene knockout or modification that inhibits 2-oxoglutarate dehydrogenase and are also capable of overexpressing 2-oxoglutarate: affected by Body oxidoreductase (2-oxoglutarate: acceptoroxidoreductase). In additional embodiments, the genetically engineered A. succinogenes further comprises modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter.

The present invention provides a method for producing glutamate (glutamate), comprising: providing genetically engineered A. succinogenes comprising one or more gene knockouts or modifications inhibiting C3 enzymes selected from the group consisting of The group consisting of pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; in said growth medium The genetically engineered A. succinogenes were grown to produce glutamate.

The present invention provides a method for producing glutamic acid, comprising: providing genetically engineered A. succinogenes capable of overexpressing a C4 enzyme selected from the group consisting of succinyl-CoA synthetase, PEPCK, malate dehydrogenation Enzymes, the group consisting of fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium .succinogenes to produce glutamate.

The present invention provides the method for producing glutamic acid, comprising: A.succinogenes of genetic engineering is provided, and it comprises one or more gene knockouts that suppress C3 enzyme or modification and can overexpress C4 enzyme, described The C3 enzyme is selected from the group consisting of pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, the C4 enzyme is selected from the group consisting of succinyl-CoA synthetase, PEPCK, malate dehydrogenase, The group consisting of fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium to produce glutamic acid.

[0037] In additional embodiments, the genetically engineered A.succinogenes further comprise gene knockout or modification that inhibits 2-oxoglutarate dehydrogenase and are also capable of overexpressing 2-oxoglutarate: affected by body oxidoreductase. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing glutamate dehydrogenase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase Group. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode dicarboxylate transporters.

[0038] The present invention provides a method for producing aspartate, comprising: providing genetically engineered A.succinogenes comprising one or more gene knockouts or modifications that inhibit C3 enzymes, wherein the C3 enzymes are selected from from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; culturing said growth medium in said growth medium Genetically engineered A. succinogenes to produce aspartic acid.

[0039] The present invention provides a method for producing aspartic acid, comprising: providing genetically engineered A. succinogenes capable of overexpressing a C4 enzyme selected from the group consisting of succinyl-CoA synthetase, PEPCK, malate Hydrogenase, fumarase, fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium to produce aspartic acid.

The present invention provides the method for producing aspartic acid, comprising: A.succinogenes of genetic engineering is provided, and it comprises one or more gene knockouts that suppress C3 enzyme or modify and can overexpress C4 enzyme, so The C3 enzyme is selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, and the C4 enzyme is selected from succinyl-CoA synthetase, PEPCK, malate dehydrogenase, fumarase , fumarate reductase, and combinations thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and cultivating said genetically engineered A. succinogenes in said growth medium to produce aspartate acid.

[0041] In additional embodiments, the genetically engineered A.succinogenes further comprise gene knockout or modification that inhibits 2-oxoglutarate dehydrogenase and are also capable of overexpressing 2-oxoglutarate: affected by body oxidoreductase. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing glutamate dehydrogenase. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing aspartate aminotransferase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes capable of increasing flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprises modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase flux through the PPP. In another embodiment, the genetically engineered A. succinogenes further comprises a gene knockout or modification to inhibit malic enzyme. In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification to inhibit oxaloacetate decarboxylase. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter.

The present invention provides a method for producing aspartic acid, comprising: providing genetically engineered A.succinogenes capable of overexpressing C4 enzymes selected from the group consisting of PEPCK, malate dehydrogenase, fumarase, and a combination thereof; providing a growth medium for culturing said genetically engineered A. succinogenes; and culturing said genetically engineered A. succinogenes in said growth medium to produce aspartic acid.

The present invention provides the method for producing aspartic acid, comprising: A.succinogenes of genetic engineering is provided, and it comprises one or more gene knockouts that suppress C3 enzyme or modification and can overexpress C4 enzyme, so The C3 enzyme is selected from pyruvate kinase, pyruvate-formate lyase, pyruvate dehydrogenase, and combinations thereof, and the C4 enzyme is selected from PEPCK, malate dehydrogenase, fumarase, and combinations thereof; a growth medium for culturing said genetically engineered A. succinogenes; and culturing said genetically engineered A. succinogenes in said growth medium to produce aspartic acid.

[0044] In another embodiment, the genetically engineered A. succinogenes further comprises gene knockout or modification inhibiting fumarate reductase. In additional embodiments, the genetically engineered A. succinogenes are capable of overexpressing aspartate ammonia-lyase. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes capable of increasing flux through the EMP pathway. In additional embodiments, the genetically engineered A. succinogenes further comprise modification of one or more genes capable of bridging the C4 and C3 pathways selected from the group consisting of malic enzyme and oxaloacetate decarboxylase Group. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that increase flux through the PPP. In additional embodiments, the genetically engineered A. succinogenes further comprise modifications of one or more genes that increase substrate uptake. In additional embodiments, the one or more genes increase the uptake of a substrate selected from the group consisting of arabinose, xylose, glucose, fructose, and sucrose. In additional embodiments, the genetically engineered A. succinogenes further comprise a modification of one or more genes that enhance secretion of the chemical product. In additional embodiments, the one or more genes encode a dicarboxylate transporter.

Description of drawings

[0045] Figure 1 shows the reaction pathways for the use of A. succinogenes genes for the production of chemical products referred to herein.

[0046] Figure 2 shows the pathways for arabinose uptake and entry into central metabolism.

[0047] Figure 3 shows the pathways for xylose uptake and entry into central metabolism.

[0048] Figure 4 shows the pathways for fructose uptake and entry into central metabolism.

Detailed ways

[0049] All patents, patent applications, government publications, government regulations, and literature references cited in this specification are hereby incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control.

[0050] The production of bio-based chemicals is a growing industry, and some bio-based chemicals are displacing fossil fuel-based markets. A. succinogenes are promising candidates for industrial succinic acid production as well as other chemical products including malic acid, fumaric acid, 5-aminolevulinic acid, 2-ketoglutaric acid, glutamic acid, and aspartic acid. Bio-catalyst. In addition to producing succinate, wild-type A. succinogenes 130Z also produced considerable amounts of unwanted formate, acetate, and ethanol. A. succinogenes fermentative metabolism splits at phosphoenolpyruvate (PEP) and/or pyruvate into a C4 branch producing succinate and a C3 branch producing formate, acetate, and ethanol. Improving succinate production in A. succinogenes, allowing the production of other chemical products from the C4 pathway, would likely involve inhibiting and overexpressing C3 pathway enzymes. In addition, enzymatic activities that can bridge the C4 and C3 pathways (such as malic enzyme and oxaloacetate decarboxylase) can also be inhibited. As C4 pathway flux increases, so does the flux supplying substrate to the C4 pathway. Genes encoding specific enzymes involved in sugar uptake, glycolysis, PPP, Entner-Doudoroff pathways can be inhibited or overexpressed to increase throughput. In addition, chemical product secretion can be enhanced by overexpressing dicarboxylic acid transporters.

[0051] A. succinogenes is a bacterium as a potential biocatalyst for industrial succinic acid production. Wild-type A. succinogenes 130Z also produced substantial amounts of unwanted formic acid, acetic acid, and ethanol. A. succinogenes fermentative metabolism splits at (PEP and/or pyruvate) the C4 branch producing succinate and the C3 branch producing formate, acetate, and ethanol. Improving succinate production in A. succinogenes would likely involve inhibition of C3 pathway enzymes , and overexpress C4 pathway enzymes. The C4 pathway is not only important for the production of succinate, but other commercially important chemical products are also intermediates of the C4 pathway or can be derived from the C4 pathway. These chemical products include malic acid, fumaric acid, 5 -Aminolevulinic acid, 2-ketoglutarate, glutamic acid, and aspartic acid. According to DOE/JGI, the A. succinogenes 130Z (ATCC 55618) genome is being sequenced and the draft sequence (draft sequence) is currently available from Obtained from the Zeikus lab. To aid in the design of strategies for the production of C4-related compounds, we have predicted what genes are important and have identified the corresponding open reading frames (ORFs) present in the draft sequence so that they Can be protected when used in the preparation of chemical products.

Engineering A. succinogenes to produce chemical products (e.g., succinic acid, fumaric acid, malic acid, 5-aminolevulinic acid, 2-ketoglutaric acid, glutamic acid) by overexpressing and/or suppressing appropriate genes , and aspartic acid) are advantageous for the following reasons: (a) by knowing the gene sequence, they can be overexpressed, knocked out, or altered to benefit chemical product production. This is a more direct and efficient approach than chemical mutagenesis and selection pressure; (b) it increases succinate production beyond that of the wild-type strain; (c) unlike the wild-type strain, the novel, engineered strain does not produce unwanted end products; (d) it allows the production of new chemical products from A. succinogenes; and (e) A. succinogenes chemical product production is based on renewable resources and can compete with and potentially replace fossil fuel-based Chemical Products Market.

[0053] Production of chemical products utilizing the C4 pathway of A. Succinogenes requires overexpression of C4 enzymes, inhibition of C3 enzymes, and in some cases, inhibition of some C4 enzymes. Figure 1 shows the pathways of these reactions. The A. succinogenes genes that catalyze the enzymes can be engineered to maximize the production of the various chemical products of the pathway. Thick arrows represent C4 reactions. Thin arrows represent C3 reactions, except for 1 and 20. A reaction that decarboxylates malate to pyruvate is also thought to exist, but the gene has not been identified so far. The following abbreviated and numbered designations are used in the figures: Glc, glucose; PEP, phophoenolpyruvate; OAA, oxaloacetate; Mal, malate; Fum, fumarate; Suc, succinate; SucCoA, Succinyl-CoA; Gly, glycine; ALA, 5-aminolevulinic acid; 2oxo, 2-oxoglutarate; Glu, glutamic acid; Pyr, pyruvate; For, formic acid; AcCoA, acetyl-CoA; Acetaldehyde; EtOH, ethanol; AcPi, acetyl-phosphate; Ace, acetic acid. 1, glycolytic enzyme; 2, PEP carboxykinase; 3, malate dehydrogenase; 4, fumarase; 5, fumarate reductase; 6, succinyl-CoA synthetase; 7, glutamyl-tRNA reductase; 8,2-ketoglutarate: receptor oxidoreductase; 9,2-ketoglutarate dehydrogenase; 10, glutamate dehydrogenase; 11, pyruvate kinase; 12, pyruvate formate lyase ; 13, pyruvate dehydrogenase; 14, acetaldehyde dehydrogenase; 15, alcohol dehydrogenase; 16, aldehyde dehydrogenase; 17, phosphate acetyl-transferase (phosphate acetyl-transferase); 18, acetate kinase (acetatekinase); 19, acetylphosphatase; 20, oxaloacetate decarboxylase.

[0054] Overexpression of enzymes may include overexpression of native enzymes or overexpression of enzymes from other bacteria. Enzymes can be overexpressed in A. succinogenes as described by members of our laboratory in Kim et al. 2004 Plasmid 51: 108-115. With increased production of chemical products, flux to PEP branch points becomes the limiting factor, in which case enzymes involved in sugar uptake and glycolysis also need to be overexpressed.

[0055] When inhibiting or knocking out enzymes, care is taken to ensure that cells get sufficient essential intermediates (eg, acetyl-CoA, pyruvate, succinyl-CoA) for growth and maintenance. Alternative pathways that sever or leave open to form a bypass between the C3 and C4 pathways may also be important, such as oxaloacetate decarboxylase, from 74868-75137, 75157-76965, and 76976- on contig 169 3 ORF codes for 78283.

C4 enzymes identified include, but are not limited to: phosphoenolpyruvate carboxykinase (PEPCK) encoded by pckA (PEPCK number #: AY308832.1 GI: 34582584); Malate dehydrogenase encoded by mdh (malate dehydrogenase number #: AY773261.1 GI: 54873671); fumarase encoded by contig 92 from 3526-4920; contig 120 from 8954-10744, 10896-11541, Fumarate reductase encoded by 4 ORFs 11538-11927, 11947-12291; succinyl-CoA synthetase beta subunit encoded from 44853-46013 on contig 154. The alpha subunit is truncated because the sequence extends beyond the edge of the contig. The 88 amino acids of the α subunit are present at 266-1 of contig 154; 2-oxoglutarate is encoded by three ORFs from 43546-44664, 40638-43463 on contig 154, and 4439-5863 on contig 100 Hydrogenase.

[0057] C3 enzymes identified include, but are not limited to: pyruvate kinase encoded from 7757-9193 on contig 122; pyruvate formate lyase encoded from 1-2023 on contig 126. Note: This gene was truncated due to extension beyond the end of the contig; pyruvate dehydrogenase encoded by three ORFs on contig 100 from 2-2437, 2498-4381, and 4439-5863. NOTE: The final ORF is the same as that of 2-oxoglutarate dehydrogenase. Both enzymes share the same subunit (part E3); acetaldehyde dehydrogenase encoded from 211-2835 on contig 135; alcohol dehydrogenase encoded from 4399-5517 on contig 164; encoded by contig 64 Acetyl phosphatase encoded from 3935-4207; acetate kinase from 1070-3 on contig 93. Note: About 70 amino acids were deleted due to the ORF extending beyond the edge of the contig; aldehyde dehydrogenation from 1656-1886 and 1883-3139 encoded on contig 83 to convert acetaldehyde and NAD to acetate and NADH enzyme.

[0058] The ORF encoding the phosphoacetyl-transferase cannot currently be located, but is believed to be present based on examination of closely related genomes and the considerable amount of acetate produced by A. succinogenes.

Other important enzymes include, but are not limited to: glutamate dehydrogenase (glutamate dehydrogenase) encoded from 12909-14258 on contig 159; transaminase); and aspartate ammonia-lyase (aspartate ammonia-lyase) encoded from 5361-3934 on contig 148.

Strategies for the production of chemical products based on the identified A. succinogenes genes:

I. Production of succinic acid:

[0060] Knockout or inhibition of C3 enzymes, which may or may not include: pyruvate kinase, pyruvate-formate lyase, and pyruvate dehydrogenase. C4 enzymes are overexpressed, which may include some or all of PEPCK, malate dehydrogenase, fumarase, and fumarate reductase. Recent experiments in the laboratory have shown that A. succinogenes has the greatest C4 flux that must be overcome for industrial succinate production (McKinlay et al. 2005. Actinobacillus succinogenes shows α-ketoglutarate auxotrophy in chemically defined growth medium. 2005., Appl. Environ. Microbiol. 2005. 71:6651-6656).

II. Production of fumaric acid:

[0061] The same method is used for succinate production, but fumarate reductase should be knocked out or inhibited additionally.

III. Production of malic acid:

[0062] The same method as for fumaric acid production is used, but fumarase should be knocked out or inhibited additionally.

IV. Production of 5-aminolevulinic acid:

[0063] The same method is used for succinate production, but succinyl-CoA synthetase should also be overexpressed. Only the complete β subunit of A. succinogenes succinyl-CoA synthetase has been discovered so far, but the remainder of the α subunit is expected to be present in the complete genome sequence. If the complete α-subunit is not present in the complete genome sequence, then a foreign gene is required. The hemA gene (e.g., from Rhodobacter sphaeroides, which is described in van der Werfand Zeikus. 1996. Appl. Environ. Microbiol. 62(10): 3560-3566) can also be overexpressed to convert glycine and succinyl-CoA It is 5-aminolevulinic acid. In addition, overexpression of enzymes leading to glycine synthesis may be beneficial.

V. Production of 2-ketoglutaric acid:

[0064] The same approach was used as for 5-aminolevulinic acid, but without overexpression of the glycine-producing pathway or hemA. 2-oxoglutarate dehydrogenase is unidirectional from 2-oxoglutarate to succinyl-CoA, so we can inhibit or knock it out and overexpress 2-oxoglutarate:receptor oxidoreductase (eg, from Helicobacter pylori), which function in the reverse Krebs cycle.

VI. Production of glutamic acid:

[0065] The same approach as for 2-oxoglutarate was used, but also included overexpression of glutamate dehydrogenase, encoded on contig 159 from 12909-14258.

VII. Production of Aspartic Acid:

[0066] Two methods can be used to produce aspartic acid. In the first approach, the same approach was used for glutamate, but also included overexpression of aspartate aminotransferase (encoded on contig 154 from 13745-14935). In the second approach, the same approach was used for fumarate production, but also included the overexpression of aspartate ammonia-lyase, encoded by contig 148 from 5361-3934.

[0067] A. succinogenes gene sequences derived from the A. succinogenes genome sequence and important for chemical product production have been identified. After the genome sequence is complete, we will revise the sequence to correct for low sequence quality, identify gene regions that are missing from the draft genome sequence, and identify all gene sequences missing from the draft genome sequence that may be important for chemical product production . We have manually annotated all open reading frames (ORFs) in the draft genome sequence. When a full genome sequence is complete, each ORF is annotated by a different person.

[0068] The gene was amplified by polymerase chain reaction, and the gene sequence was cloned into a plasmid by methods known in the art. Once the genes are in a plasmid, they can be overexpressed, suppressed, and can be used to knock out the genes as described above. Selection of the exact genes to be overexpressed or repressed is guided in part by metabolic studies, including in McKinlay et al. (Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analysis of ¹³ C-labeled metabolic product isotopomers.Submitted for publication) ¹³ C-labeling experiments described in . These labeling experiments are described in McKinlay et al. 2005 (Actinobacillus succinogeneses shows α-ketoglutarate auxotrophy in a chemically defined growth medium, Appl. Environ. Microbiol. 71 (11): 6651-6656) and US Patent Application No. _ to Zeikus et al. ______________ (U.S. Patent Application No. ________________, claiming priority to U.S. Provisional Application No. 60/717,425, MSU 4.1-758), the entire contents of which are incorporated by reference way incorporated into this article. These genetic tools were used to overexpress genes in A. succinogenes described in Kim et al. 2004 Plasmid 51: 108-115 and US Patent Application Serial No. 10/911,961. We can improve gene overexpression in this approach and we have been actively exploring efficient systems for gene knockout in A. succinogenes. The gene to be knocked out can be interrupted by a drug resistance cassette and used to replace the uninterrupted wild-type gene in the chromosome. Each mutant was grown to assess the effect of the mutation on growth properties and chemical product production. Mutants were analyzed using13C ^- labeling studies to determine how the mutations affected the intermediary metabolism of the organism.

[0069] The following is a list of gene targets included in the identification and annotation work herein. The gene codes: malic enzyme; pyruvate carboxylase; PEP carboxylase; PEP synthase; pyruvate-ferredoxin oxidoreductase; glucokinase; glucose permease; sucrose-specific phosphotransferase System; β-fructofuranosidase; 6-phosphogluconate dehydratase (Entner-Doudoroff pathway); ribulose-phosphate 3-epimerase (PPP); malate synthase; isocitrate cleavage Enzymes; isocitrate dehydrogenase; aconitase; citrate synthase; citrate lyase; lactate dehydrogenase; carbonic anhydrase; β-galactosidase; ATP-dependent maltose transport system; LacI repressor protein; arabinose transcriptional activator protein AraC; xylose operon activator protein, XylR; cAMP receptor protein, CRP, also known as degradant activator protein, CAP; and carbon storage regulatory protein, CsrA.

[0070] Genes of the A. succinogenes C3 and C4 pathways can be used to produce a variety of chemical products. Since these strategies lead to high rates of enhanced chemical production, the pathways by which substrates are delivered to the C4 pathway can limit the rate of chemical production. In some embodiments of the invention, flux through glycolysis, PPP, and substrate uptake is increased by inhibiting, knocking out, overexpressing, and modifying/evolving specific enzymes involved in these pathways. In yet another embodiment of the invention, chemical product secretion is increased by targeting a gene encoding a dicarboxylic acid transporter. The ORFs in the A. succinogenes draft genome sequence encoding these genes are provided so that they can be used to prepare chemical products.

[0071] Increasing C4-pathway flux can lead to a situation where the flux to deliver substrates to the C4 pathway is limiting. When this occurs, genes involved in (i) glycolysis, or EMP, (ii) PPP, and (iii) substrate uptake (eg, glucose, xylose, arabinose, sucrose) must be affected. Figures 2, 3 and 4 show the pathways of arabinose, xylose, and fructose uptake and entry into central metabolism, respectively. Furthermore, chemical product secretion may become limiting, whereby genes involved in the export of dicarboxylic acids may also need to be affected. The specific genes to be affected and the manner in which they are affected are guided in part by metabolic studies, including those described in McKinlay et al. (Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analysis of ¹³ C-labeled metabolic product isotopomers. Submitted.) ¹³ C-labeling studies. These labeling experiments were based on McKinlay et al. 2005 (Actinobacillus succinogenes shows α-ketoglutarate auxotrophy in chemically defined growth medium, Appl. Environ. Microbiol., 71 (11): 6651-6656) and US Patent Application No. _ to Zeikus et al. _______________ (US Patent Application No. ___________, claiming priority to US Provisional Application No. 60/717,425, MSU 4.1-758) with our chemically defined growth medium for A. succinogenes. For example, the flux distribution between EMP and PPP and the extent to which gluconeogenesis occurs should be revealed ^by13C -labeling experiments. If there is a substantial amount of gluconeogenesis flux, then we will knock out those enzymes that are thought to be involved in gluconeogenesis and see the effect. Furthermore, for the production of 5-aminolevulinic acid, glycine is required as a substrate. It may be valuable to increase the flux of glycine for 5-aminolevulinate production.

[0072] We have identified gene sequences believed to encode specific enzymes in the A. succinogenes 130Z draft genome sequence that we expect to be critical for altering (i) EMP flux, (ii) PPP flux, (iii) substrate uptake , (iv) dicarboxylic acid transport, (v) and glycine synthesis are important. These gene sequences are attached as attachments and summarized below. After the release of the complete A. succinogenes genome sequence, these sequences were modified because the draft genome sequence contained gaps and regions of poor sequence quality.

(i) EMP gene:

The phosphoglucose isomerase (EC 5.3.1.9) from 14164-15858 on contig 135

6-phosphofructokinase (EC 2.7.1.11) from 33518-34483 on contig 163

The triose phosphate isomerase (EC 5.3.1.1) from 16095-15022 on contig 154

The triose phosphate isomerase (EC 5.3.1.1) from 40514-41299 on contig 154

The triose phosphate isomerase (EC 5.3.1.1) from 42329-43099 on contig 163

Fructose-1,6-diphosphatase (EC 3.1.3.11) (gluconeogenesis) from 2747-3760 on contig 74

[0079] Two ORFs from 376-1 on contig 138 encode the N-terminus of fructose 1,6-bisphosphate aldolase. Note: the complete sequence is not a known ORF extending beyond the edge of the contig. The sequence was split into 2 ORFs due to low sequence quality causing frameshifts. (Fructose intake pathway).

[0080] Glyceraldehyde-3-phosphate dehydrogenase from 13295-12330 on contig 127 (EC 1.2.1.12). Note: The expected 60 bases encoding about 20 amino acids were deleted due to the 5'-end of this ORF extending beyond the edge of the contig.

3-phosphoglycerate kinase (EC 2.7.2.3) from 1655-480 on contig 138

Phosphoglycerate mutase (EC 5.4.2.1) from 4053-3370 on contig 133

The phosphoglycerate mutase (EC5.4.2.1) from 48008-48652 on contig 162

[0084] Enolase from 33951-33061 on contig 157 (EC 4.2.1.11). NOTE: This is only the C-terminus of Enolase. The region encoding the N-terminus extends beyond the edge of the contig.

Potential EMP strategies:

[0085] To increase the flux of substrates (ie, PEP and pyruvate) delivered to the C4-pathway, it may be desirable to overexpress some or all of the EMP enzymes. The extent to which each EMP enzyme affects C4-pathway flux can be estimated by measuring EMP flux (determined using ¹³ C-labeling experiments) and enzyme activity and calculating using metabolic control assays. As an alternative, EMP flux can be supplemented by Entner-Doudoroff (ED) pathway flux, providing two pathways for substrate delivery to the C4 pathway. The gene encoding the A. succinogenes ED enzyme 6-phosphogluconate dehydratase (EC 4.2.1.12) was not found in the draft genome sequence, so if it is absent in the complete genome sequence, it may The foreign gene encoding this enzyme must be overexpressed. The gene encoding A. succinogenes ED enzyme 2-keto-3-deoxy-6-phosphogluconate aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) (EC 4.2.1.14) was obtained from contig 148 Found from 15090-14452 on 19472-18834 and contig 159.

(ii) PPP gene:

Glucose-6-phosphate dehydrogenase (EC1.1.1.49) from 10225-8738 on contig 146

6-phosphogluconolactonase (EC3.1.1.31) from 8607-7906 on contig 115

6-phosphogluconate dehydrogenase (EC1.1.1.44) from 5548-4094 on contig 115

6-phosphogluconate dehydrogenase (EC1.1.1.44) from 5548-4094 on contig 141

[0090] Ribulose-phosphate 3-epimerase (EC 5.1.3.1) was not found on any contig, but is likely to be found in the complete genome sequence since the closely related Pasteurellaceae have this gene.

The ribose-5-phosphate isomerase (EC5.3.1.6) from 20162-19710 on contig 154

The ribose 5-phosphate isomerase (EC5.3.1.6) from 37092-36643 on contig 154

The ribose 5-phosphate isomerase (EC 5.3.1.6) from 2978-2337 on contig 56

The ribose 5-phosphate isomerase (EC5.3.1.6) from 64945-64286 on contig 168

Ketotransferase N-terminal subunit (EC2.2.1.1) from 37734-36904 on contig 159

Ketotransferase C-terminal subunit (EC2.2.1.1) from 36911-35976 on contig 159

[0097] Contig 86 from 2744-738 ketose transferase (EC 2.2.1.1).

Aldehyde carboxyltransferase (transaldolase) (EC2.2.1.2) from 6033-5368 on contig 105

The aldehyde carboxyltransferase (EC 2.2.1.2) from 12149-13102 on contig 135

[00100] Potential PPP strategy: Flux through PPP causes 1/6 of carbon to be lost as _CO2 , not all carbon can be recovered in C4 pathway. In the presence of a substantial amount of PPP flux (to be determined ^by13C -labeling studies), maximizing carbon recovery in chemical product production requires knockdown of the glucose-6-phosphate dehydrogenase, or if the ED pathway is required flux, knock out 6-phosphogluconate dehydrogenase. These two enzymes are part of the oxidative PPP. By diverting a minimal amount of flux through the non-oxidative PPP to produce the most basic metabolic intermediate without losing carbon as _CO , the cells should still be able to survive, as has been shown in the case of E. coli (Zhao et al. al. FEMS Microbiol. Lett. 2003.220: 295-301 and Hua et al. J. Bacteriol. 2003.185(24): 7053-7067).

[00101] Alternatively, if the economics of the industry allow for the loss of some CO _or if the source of reducing power (required for the C4 pathway) is expensive, it is worthwhile to divert the flux through the oxidative PPP, which provides 2 CO per glucose NADPH. For this, the EMP enzyme, phosphoglucoisomerase, can be knocked out. Conversion of the resulting NADPH to NADH by natural or exogenous transhydrogenases or diaphorases may also be necessary, as is the case for E. coli phosphoglucoisomerase knockout mutants (Canonaco et al. al. FEMS Microbiol. Lett. 2001. 204: 247-252). A. succinogenes transhydrogenases are encoded by contig 152 from 24659-26191, contig 152 from 26203-27654 and possibly contig 107 from 11481-10150.

(iii) Substrate uptake genes:

(a) Arabinose: ABC-type arabinose transport system periplasmic protein from 6574-7389 on contig 155; ABC-type arabinose transport system ATP-binding protein from 7413-8948 on contig 155; contig 155 ABC-type arabinose transport system permease component from 8964-9932 on contig 155; L-arabinose isomerase (EC 5.3.1.4) from 12637-14124 on contig 155; L-arabinose isomerase from 3876-2419 on contig 117 Xylulokinase (EC 2.7.1.53); L-xylulokinase (EC 2.7.1.53) from 2250-3704 on contig 106; ribulose-5-phosphate-4 from 2782-3474 on contig 115 - Epimerase (EC 5.1.3.4).

(b) xylose: xylose isomerase (EC 5.3.1.5) from 21265-22578 on contig 155; D-xylulokinase (EC 2.7.1.17) from 43481-41883 on contig 165 or L-ribulokinase (EC 2.7.1.16); D-xylulokinase (EC 2.7.1.7) from 22646-24091 on contig 155; D-xylulone from 11012-12616 on contig 155 Glycokinase (EC 2.7.1.7); ABC-type xylose or ribose transporter ATP-binding protein from 40497-38974 on contig 155; ABC-type xylose transporter periplasmic protein from 21013-20015 on contig 155 ( xylose transporter periplasmic protein); permease component of the ABC-type xylose transport system from 18411-17284 on contig 155; ATP-binding component of the ABC-type xylose transport system from 19929-18415 on contig155; from 44518 on contig 165 -45636 xylose operon repressor.

(c) Glucose: the glucokinase transcription regulator (EC 2.7.1.2) from 4340-3426 on contig 99; The glucose-specific PTS protein IIA from 411-13 on contig 153; From 27639- on contig 153 Glucose or β-glucoside-specific PTS protein IIC at 29498; glucose or β-glucoside-specific PTS protein IIA from 40420-39968 on contig 165.

(d) fructose: the fructose-specific PTS protein IIC from 5534-3858 on contig 106; The fructose-specific PTS protein (IIA/FPr) from 7985-6483 on contig 106; On contig 135 from 9001- Fructose-specific PTS protein IIC from 7952; Fructose-specific PTS protein IIB from 9331-9014 on contig 135; Mannitol/fructose-specific PTS protein IIA from 9799-9353 on contig 135; From 6480- on contig 106 1-Phosphofructokinase (EC 2.7.1.56) on 5539; 1-Phosphofructokinase (EC2.7.1.56) from 6480-5539 on contig 54; D-fructokinase from 10269-11198 on contig 168.

(e) General: PEP phospho-transferase system enzyme I (EI) from 2202-475 on contig 153; Phosphotransferase system, HPr-related protein on contig 153; From 41787-40432 on contig 165 Proton/sugar symport protein.

[00107] (f) Sucrose: sucrose invertase from 11272-12720 on contig 168.

(g) sugar flows out: the potential major facilitator superfamily sugar permease (potential major facilitator superfamily sugar permease) from 25395-26780 on contig 145; Main facilitation superfamily sugar permease from 16827-15997 on contig 123 Permease.

[00109] Substrate uptake strategy: A variety of substrates are important for the production of chemical products by A. succinogenes, including those that are abundant in common feedstocks, arabinose, xylose, glucose, fructose, and sucrose. All of these sugars are most likely taken up by phosphotransferase systems (PTS), kinases, ABC-type transport systems or by catabolic diffusion (ie proton symporting permeases). Ideally, we would like to take up each sugar by permease in order to release more ATP that the cells need. If this is not possible, other systems are more favorable than PTS because PTS converts PEP to pyruvate, which may or may not be readily carboxylated to oxaloacetate or malate.

[00110] Arabinose is most likely internalized by an ABC-type transport system. Once internalized, it can enter metabolism through a number of pathways (Figure 1). ^Using13C -arabinose should reveal which pathway is most important. One, some, or all genes in this pathway may need to be overexpressed.

到PEP的通量。此外，我们已经鉴定了似乎是木糖操纵子阻遏蛋白的物质。在存在木糖的情况下敲除这个阻遏物可能是值得的，以便使木糖摄取最大化。[00111] Xylose is most likely internalized by an ABC-type transport system and enters central metabolism in PPP as xylulose-5-phosphate (Figure 2). The proposed knockdown of PPP would not be deleterious if xylose and glucose were co-metabolized, since xylose enters the metabolic downstream of 6-phosphogluconate dehydrogenase. However, overexpression of PPP downstream of xylulose

Flux to PEP. Furthermore, we have identified what appears to be the repressor protein of the xylose operon. It may be worth knocking out this repressor in the presence of xylose in order to maximize xylose uptake.

[00112] Glucose may be taken up by the PTS system or by hexokinase, which has not been identified but whose activity has been reported by our laboratory members Pil Kim (Kim and Vieille. Glucose phosphorylation mechanisms in succinate-producing Actinobacillus succinogenes. Submitted) and Mariet Detected by van der Werf (Van der Werf et al. Arch. Microbiol. 1997.167: 332-342). If efficient carboxylation of pyruvate to PEP proves difficult, then we would want to knock out the PTS system and overexpress hexokinase.

[00113] Fructose is likely taken up by PTS or fructokinase and enters glycolysis as fructose-6-phosphate or fructose 1,6-bisphosphate and glyceraldehyde 3-phosphate depending on the uptake system (Figure 3). As with glucose, we will most likely wish to knock out the PTS system and overexpress fructokinase. Since fructose enters metabolism early in glycolysis, the proposed phosphoglucose isomerase mutations that increase PPP flux should not be used in cases where fructose is the major carbon source.

[00114] If sucrose is present, we would want to overexpress sucrose invertase to efficiently convert sucrose to glucose and fructose.

[00115] To improve chemical product production, we also wish to knock out genes that repress substrate uptake, such as sugar efflux genes. However, depending on what sugars these sugar efflux permeases act on, knocking them out may not be necessary. Indeed, if a sufficient sugar concentration gradient could cause these sugar efflux permeases to take up sugar, it might be worthwhile to increase their sugar uptake capacity by overexpressing the genes.

(iv) Dicarboxylate transporter genes include:

[00116] TRAP C4-dicarboxylate transport (Dct) system proteins from 18087-16792 on contig 154. Large subunit of the TRAP-type C4-dicarboxylic acid transport system from 20342-19050 on Contig 159

[00117] TRAP-type C4-dicarboxylic acid transport system small permease component of 7334-6057 on contig 117

[00118] TRAP-type C4-dicarboxylic acid transport system large protein component of contig 122 from 13540-12269

[00119] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 27696-26689 on contig 148 (dicarboxylic acid-binding protein)

[00120] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 21876-20890 on contig 159 (dicarboxylic acid-binding protein)

[00121] TRAP-type C4-dicarboxylic acid transport system small permease components from 14033-13551 on contig 122

[00122] TRAP-type C4-dicarboxylic acid transport system small permease components from 20836-20345 on contig 159

[00123] TRAP-type C4-dicarboxylic acid transport system small permease component from 18563-18087 on contig 154

[00124] TRAP-type C4-dicarboxylic acid transport system small permease components from 26666-26157 on contig 148

[00125] Small permease components of the TRAP-type C4-dicarboxylic acid transport system from 12484-12975 on contig 148

[00126] TRAP-type C4-dicarboxylic acid transport system small permease component from 7813-7331 on contig 117

[00127] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 19646-18669 on contig 154 (dicarboxylic acid-binding protein)

[00128] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 12245-11259 on contig 148 (dicarboxylic acid-binding protein)

[00129] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 15018-14053 on contig 122 (dicarboxylic acid-binding protein)

[00130] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 5984-4998 on contig 117

[00131] Periplasmic components of the TRAP-type C4-dicarboxylic acid transport system from 4938-3949 on contig 117

[00132] Integral membrane components of the TRAP-type C4-dicarboxylic acid transport system from 4284-3718 on contig 77. Note: The N-terminal coding region is missing because it extends beyond the edge of the contig.

[00133] TRAP-type C4-dicarboxylic acid transport system integral membrane components from 26147-24867 on contig 148

[00134] TRAP-type C4-dicarboxylic acid transport system integral membrane components from 12987-14288 on contig 148

[00135] TRAP-type C4-dicarboxylic acid transport system components from 18563-18087 on contig 154

Dicarboxylate transporter strategy:

[00136] Since there are many candidates for dicarboxylic acid transport, we determined which genes were important for specific chemical product production by sequentially knocking out each gene and examining the effect on specific chemical product productivity. Once suitable genes have been identified for the secretion of specific chemical products, we will overexpress these genes.

(v) Glycine synthesis gene

[00137] Serine deaminase or threonine ammonia lyase (EC 4.3.1.19), located on contig141 from 10270-11808

[00138] Glycine hydroxymethyltransferase (EC2.1.2.1) from 2950-1682 on contig 137

[00139] If glycine availability was found to limit 5-aminolevulinic acid production, then these genes would all be overexpressed.

Table 1. Nucleotide sequences and open reading frames (ORFs) of the identified Actinobacillus succinogenes genes.

phosphoenolpyruvate carboxykinase

malate dehydrogenase

fumarase

fumarate reductase

succinyl-CoA synthetase

glutamyl-tRNA reductase

2-oxoglutarate dehydrogenase

glutamate dehydrogenase

aspartate ammonia lyase

aspartate aminotransferase

pyruvate kinase

pyruvate dehydrogenase

pyruvate-formate lyase

Aldehyde dehydrogenase

alcohol dehydrogenase

Acylphosphatase

Aldehyde dehydrogenase

Acetate kinase

Phosphoacetyl-transferase

oxaloacetate decarboxylase

Example 1

[00140] According to DOE/JGI, the A. succinogenes 130Z (ATCC 55618) genome was sequenced and the sequence was used to identify genes important for the production of C4-related compounds. The sequences of these genes are disclosed herein as SEQ ID NO: 1 to SEQ ID NO: 119.

[00141] The nucleotide sequences of the genes and open reading frames (ORFs) of the A. succinogenes C3 and C4 pathways are disclosed below. A. succinogenes 130Z can be purchased from the American Type Culture Collection (ATCC). The genomic DNA sequenced here was from a strain obtained from ATCC. Both pckA encoding PEPCK and mdh encoding malate dehydrogenase have been expressed in E. coli. PEPCK has been overexpressed in A. succinogenes.

[00142] SEQ ID NO: 1 is the gene sequence of A. succinogenes encoding PEPCK. (PEPCK number #: AY308832.1 GI: 34582584 > gi|34582584|gb|AY308832.1|A. succinogenes PEPCK gene, complete cds).

[00143] SEQ ID NO: 2 is the ORF encoding A. succinogenes malate dehydrogenase on Contig 160 (laboratory member Maris Laivenieks previously sequenced this gene and pckA encoding PEPCK) EC 1.1.1.37 > SPTR top hit:' Q5U907 NAD(H)-dependent malate dehydrogenase-41924:42862.

[00144] SEQ ID NO: 3 is malic acid DH number #: AY773261.1 GI: 54873671 > gi|54873671|gb|AY773261.1|A. succinogenes plasmid pKNJNAD(H)-dependent malate dehydrogenase (mdh) gene, complete cds

[00145] SEQ ID NO: 4 is the ORF EC 4.2.1.2 (> SPTR top hit: 'Q65UJ3 FumC protein. Mannheimiasuc-3526: 4920) of encoding A. succinogenes fumarase on Contig 92.

[00146] The ORF encoding A. succinogenes fumarate reductase on Contig 120: frdB was split into 2 overlapping ORFs (FRD iron-sulfur protein and frdB). EC 1.3.99.1 or 1.3.5.1. SEQ ID NO: 5 is frdA. (>PRIAM: succinate dehydrogenase. -8954:10744). SEQ ID NO: 6 is a FRD iron-sulfur protein (>SPTR top hit: 'Q7VPM7 fumarate reductase iron-sulfur-10896:10970). SEQ ID NO: 7 is FrdB. The first 11 bases overlap with iron-sulfur protein ORF (>SPTR top hit: 'Q65S00FrdB protein. Mannheimia suc-10960:11541). SEQ ID NO: 8 is frdC. The first four bases overlap with the C-terminus of the frdB product. (>SPTR top hit: 'Q65RZ9 FrdC protein. Mannheimia suc-11538:11927). SEQ ID NO: 9 is frdD (>SPTR top hit: 'Q65RZ8 FrdD protein. Mannheimia suc-11947:12291).

[00147] ORF encoding A. succinogenes succinyl-CoA synthetase on Contig 154: EC 6.2.1.5 SEQ ID NO: 10 is a β-subunit (>PRIAM: succinate-CoA ligase [ADP-forming]. -44853:46013). The α-subunit is mostly missing, however the N-terminus is found at the downstream edge of the contig 154 β-subunit gene, where it would be expected to be located when compared to other genomes. It was discovered by blasting the α-subunit nucleotide sequence against the A. succinogenes draft genome sequence. SEQ ID NO: 11 is the sequence 260 attttaattaataaaaacactaaagtgatctgccaggg-attcaccggcggacaagg205. Surprisingly, however, the sequences did not match from 260:1. The open reading frame was taken from 260:1 without a stop codon and a BLASTp search was performed against all bacteria in the nr database (88 amino acids). The search revealed nearly identical identity to the succinyl-CoA α-subunit over all 88 amino acids queried, including the conserved CoA-binding site. SEQ ID NO：12是序列260attttaattaataaaaacactaaagtga-tctgccagggattcaccggcggacaaggcaccttccacagcgaacaggcattggcatacggcacgaaattagtgggcggtaccagtccgggtaaaggcggtacgacgcatttgggattgccggtgtttgatacggtacgcgaagcggtgcaaaaaaccggagccaccgccagcgtgatttatgtgccggcaccgttctgtaaggacgccattctggaagcgattgacgccgg 1.

[00148] ORF of A. succinogenes 2-oxoglutarate dehydrogenase encoded on contig 154: SEQ ID NO: 14 is E2 (EC 2.3.1.61) dihydrolipoamide succinyltransferase (Dihydrolipoamide succinyltransferase) >- 43546:44664. SEQ ID NO: 15 is E1 (EC 1.2.4.2) (>SPTR top hit: 'Q65SU8 SucA protein. Mannheimiasuc-40638:43463). SEQ ID NO: 16 is E3 found on Contig 100 (EC 1.8.1.4) - Note: same ORF as pyruvate dehydrogenase (this is normal) (> SPTR top hit: 'Q65SW9Lpd protein. Mannheimia succ-4439 :5863).

[00149] SEQ ID NO: 17 is the ORF (EC 1.4.1.4) of encoding A. succinogenes glutamate dehydrogenase on contig 159 (>PRIAM: glutamate dehydrogenase (NADP+).-12909:14258).

[00150] SEQ ID NO: 13 is the ORF (EC 4.3.11) of encoding A. succinogenes aspartate ammonia-lyase on contig 148 (> SPTR top hit: 'Q7VNH2 AspA protein. Haemophilus ducreyi-5361: 3934).

[00151] SEQ ID NO: 119 is the ORF (EC 2.6.1.1) of encoding A. succinogenes aspartate aminotransferase on contig 154 (> SPTR top hit: 'P44425 aspartate ammonia lyase. Haemophilus influenzae-13745: 14935).

[00152] SEQ ID NO: 18 is the ORF (EC 2.7.1.40) encoding A. succinogenes pyruvate kinase on Contig 122 (>PRIAM: pyruvate kinase.-7757:9193).

[00153] ORF encoding A. succinogenes pyruvate dehydrogenase on Contig 100: SEQ ID NO: 19 is EC 1.2.4.1 (> SPTR top hit: 'P45119 pyruvate dehydrogenase E1co-2: 2437). SEQ ID NO: 20 is EC 2.3.1.12 (>PRIAM: Dihydrolipoamide S-succinyltransferase (Dihydrolipoamide S-succinyltransferase).-2498:4381). SEQ ID NO: 21 is EC 1.8.1.4 (>SPTR top hit: 'Q65SW9 Lpd protein. Mannheimia succ-4439:5863).

[00154] SEQ ID NO: 22 is the truncated ORF-EC 2.3.1.54.1..2023bp of A. succinogenes pyruvate pyruvate formate lyase on Contig 126

[00155] SEQ ID NO: 23 is the ORF on Contig 135 encoding A. succinogenes acetaldehyde dehydrogenase. (EC 1.2.1.10) Acetyl-CoA ←→ acetaldehyde (>SPTR top hit: 'Q65QG3EutG protein. Mannheimia suc-211:2835).

[00156] SEQ ID NO: 24 is the ORF (E.C.1.1.1.1) encoding A. succinogenes alcohol dehydrogenase on contig 164 (>SPTR top hit: 'Q8DWE2 putative alcohol dehydrogenase-4399:5517).

[00157] SEQ ID NO: 25 is the ORF (EC 3.6.1.7) of Contig 64 encoding A. succinogenes acyl phosphatase (acylphosphatase) (> SPTR top hit: 'Q9CNN1 hypothetical (Hypothetical) protein PM0396.-3935: 4207) .

[00158] SEQ ID NO: 26 is the ORF EC 1.2.1.3 of Contig83 encoding A. succinogenes aldehyde dehydrogenase (> SPTR top hit: 'Q65SA2 PutA protein. Mannheimiasuc-1656: 1886).

[00159] SEQ ID NO: 27 is the ORF encoding A. succinogenes aldehyde dehydrogenase on Contig 83 (> SPTR top hit: 'Q65SA2 PutA protein. Mannheimia suc-1883: 3139).

[00160] SEQ ID NO:28 is the ORF encoding A. succinogenes acetate kinase from 1070:3 on Contig 93. There are approximately 350 amino acids (EC 2.7.2.1) out of 420 predicted amino acids.

[00161] The ORF encoding the phosphoacetyl-transferase has not been located at present, but is believed to exist based on similarities to closely related genomes and acetate production by A. succinogenes.

[00162] ORF encoding A. succinogenes oxaloacetate decarboxylase on Contig 169: EC 4.1.1.3. SEQ ID NO: 29 is oadG (>SPTR top hit: 'Q65WL3 OadG protein. Mannheimia suc-74868:75137). SEQ ID NO: 30 is oadA (>SPTR tophit: 'Q65WL4 OadA protein. Mannheimia suc-75157:76965). SEQ ID NO: 31 is oadB (>PRIAM: oxaloacetate decarboxylase.-76976:78283).

EMP gene

[00163] SEQ ID NO: 32 is the ORF of encoding A. succinogenes phosphoglucose isomerase (EC 5.3.1.9) from 14164-15858 on contig 135

[00164] SEQ ID NO: 33 is the ORF of encoding A. succinogenes 6-phosphofructokinase (EC 2.7.1.11) from 33518-34483 on contig 163

[00165] SEQ ID NO: 34 is the ORF of A. succinogenes triose phosphate isomerase (EC 5.3.1.1) from 16095-15022 on contig 154

[00166] SEQ ID NO: 35 is the ORF of A. succinogenes triose phosphate isomerase (EC 5.3.1.1) from 40514-41299 on contig 154

[00167] SEQ ID NO: 36 is the ORF of A. succinogenes triose phosphate isomerase (EC 5.3.1.1) from 42329-43099 on contig 163

[00168] SEQ ID NO: 37 is the ORF of encoding A. succinogenes fructose-1,6-bisphosphatase (EC 3.1.3.11) (gluconeogenesis) from 2747-3760 on contig 74

[00169] SEQ ID NO: 38 is the N-terminal two ORFs on contig 138 from 376-1 encoding A. succinogenes fructose 1,6-bisphosphate aldolase. Note: The complete sequence is known not to extend the ORF of the contig. The sequence was split into 2 ORFs due to frameshift due to low sequence quality. (Fructose intake route)

[00170] SEQ ID NO: 39 is the ORF encoding A. succinogenes glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) from 13295-12330 on contig 127. Note: The expected 60 bases encoding about 20 amino acids are missing due to the 5'-end of this ORF extending beyond the edge of the contig.

[00171] SEQ ID NO: 40 is the ORF of encoding A. succinogenes 3-phosphoglycerate kinase (EC 2.7.2.3) from 1655-480 on contig 138

[00172] SEQ ID NO: 41 is the ORF of the encoding A. succinogenes phosphoglycerate mutase (EC 5.4.2.1) from 4053-3370 on contig 133

[00173] SEQ ID NO: 42 is the ORF of encoding A. succinogenes phosphoglycerate mutase (EC 5.4.2.1) from 48008-48652 on contig 162

[00174] SEQ ID NO: 43 is the ORF encoding A. succinogenes enolase (EC 4.2.1.11) from 33951-33061 on contig 157. NOTE: This is only the C-terminus of Enolase. The region encoding the N-terminus extends beyond the edge of the contig.

PPP gene

[00175] SEQ ID NO: 44 is the ORF encoding A. succinogenes glucose-6-phosphate dehydrogenase (EC 1.1.1.49) from 10225-8738 on contig 146

[00176] SEQ ID NO: 45 is the ORF of encoding A. succinogenes 6-phosphogluconate dehydrogenase (EC 1.1.1.44) from 5548-4094 on contig 115

[00177] SEQ ID NO: 46 is the ORF of encoding A. succinogenes 6-phosphogluconolactonase (EC 3.1.1.31) from 8607-7906 on contig 115

[00178] SEQ ID NO: 47 is the ORF of encoding A. succinogenes 6-phosphogluconate dehydrogenase (EC 1.1.1.44) from 5548-4094 on contig 141

[00179] The ORF encoding A. succinogenes encoding ribulose-phosphate 3-epimerase (EC 5.1.3.1) was not found on any contig, but may be found in the complete genome sequence. The closely related Pasteurellaceae have this gene.

[00180] SEQ ID NO: 48 is the ORF of A. succinogenes ribose-5-phosphate isomerase (EC 5.3.1.6) from 20162-19710 on contig154

[00181] SEQ ID NO: 49 is the ORF of A. succinogenes encoding ribose 5-phosphate isomerase (EC 5.3.1.6) from 37092-36643 on contig 154

[00182] SEQ ID NO: 50 is the ORF of A. succinogenes encoding ribose 5-phosphate isomerase (EC 5.3.1.6) from 2978-2337 on contig 56

[00183] SEQ ID NO: 51 is the ORF of A. succinogenes encoding ribose 5-phosphate isomerase (EC 5.3.1.6) from 64945-64286 on contig 168

[00184] SEQ ID NO: 52 is the ORF of the encoding A. succinogenes transketolase (transketolase) N-terminal subunit (EC 2.2.1.1) from 37734-36904 on contig 159

[00185] SEQ ID NO: 53 is the ORF of the coding A. succinogenes transketolase C-terminal subunit (EC 2.2.1.1) from 36911-35976 on contig 159

[00186] SEQ ID NO: 54 is the ORF of the encoding A. succinogenes transketolase (EC 2.2.1.1) from 2744-738 on contig 86

[00187] SEQ ID NO: 55 is the ORF of encoding A. succinogenes transaldolase (EC 2.2.1.2) from 6033-5368 on contig 105

[00188] SEQ ID NO: 56 is the ORF of encoding A. succinogenes transaldolase (EC 2.2.1.2) from 12149-13102 on contig 135

(iii) Substrate uptake genes

(a) Arabinose

[00189] SEQ ID NO: 57 is the ORF of A. succinogenes arabinose transporter periplasmic protein (arabinose transporter periplasmicprotein) from 6574-7389 on contig 155

[00190] SEQ ID NO: 58 is the ORF of the A. succinogenes arabinose transporter ATP-binding protein from 7413-8948 on contig 155

[00191] SEQ ID NO: 59 is the ORF of the A. succinogenes ABC-type arabinose transport system permease component from 8964-9932 on contig 155

[00192] SEQ ID NO: 60 is the ORF of encoding A. succinogenes L-arabinose isomerase (EC 5.3.1.4) from 12637-14124 on contig 155

[00193] SEQ ID NO: 61 is the ORF encoding A. succinogenes L-xylulokinase (EC 2.7.1.53) from 3876-2419 on contig 117

[00194] SEQ ID NO: 62 is the ORF encoding A. succinogenes L-xylulokinase (EC 2.7.1.53) from 2250-3704 on contig 106

[00195] SEQ ID NO: 63 is the ORF of encoding A. succinogenes ribulose-5-phosphate-4-epimerase (EC 5.1.3.4) from 2782-3474 on contig 115

(b) Xylose

[00196] SEQ ID NO: 64 is the ORF of encoding A. succinogenes xylose isomerase (EC 5.3.1.5) from 21265-22578 on contig 155

[00197] SEQ ID NO: 65 is the ORF of encoding A. succinogenes D-xylulokinase (EC 2.7.1.17) or L-ribulokinase (EC 2.7.1.16) from 43481-41883 on contig 165

[00198] SEQ ID NO: 66 is the ORF encoding A. succinogenes D-xylulokinase (EC 2.7.1.17) from 22646-24091 on contig 155

[00199] SEQ ID NO: 67 is the ORF encoding A. succinogenes D-xylulokinase (EC 2.7.1.17) from 11012-12616 on contig 155

[00200] SEQ ID NO: 68 is the ORF of the A. succinogenes ABC-type xylose or ribose transporter ATP-binding protein from 40497-38974 on contig 155

[00201] SEQ ID NO: 69 is the ORF of the A. succinogenes ABC-type xylose transporter periplasmic protein from 21013-20015 on contig 155

[00202] SEQ ID NO: 70 is the ORF of the A. succinogenes ABC-type xylose transport system permease component from 18411-17284 on contig 155

[00203] SEQ ID NO: 71 is the ORF encoding the ATP-binding component of the A. succinogenes ABC-type xylose transport system from 19929-18415 on contig 155

[00204] SEQ ID NO: 72 is the ORF of the A. succinogenes xylose operon repressor protein encoded from 44518-45636 on contig 165

(c) Glucose

[00205] SEQ ID NO: 73 is the ORF of the encoding A. succinogenes glucokinase transcription regulator (EC 2.7.1.2) from 4340-3426 on contig 99

[00206] SEQ ID NO: 74 is the ORF of encoding A. succinogenes glucose-specific PTS protein IIA from 411-13 on contig 153

[00207] SEQ ID NO: 75 is the ORF of A. succinogenes glucose or β-glucoside (glucoside) specific PTS protein IIC from 27639-29498 on contig 153

[00208] SEQ ID NO: 76 is the ORF of A. succinogenes glucose or β-glucoside-specific PTS protein IIA from 40420-39968 on contig 165

(d) fructose

[00209] SEQ ID NO: 77 is the ORF of the coding A. succinogenes fructose-specific PTS protein IIC from 5534-3858 on contig 106

[00210] SEQ ID NO: 78 is the ORF of the coding A. succinogenes mannitol/fructose specific PTS protein (IIA) from 7985-6483 on contig 106

[00211] SEQ ID NO: 79 is the ORF of the coding A. succinogenes fructose or mannose specific PTS protein IIC from 9001-7952 on contig 135

[00212] SEQ ID NO: 80 is the ORF of the coding A. succinogenes fructose-specific PTS protein IIB from 9331-9014 on contig 135

[00213] SEQ ID NO: 81 is the ORF of A. succinogenes mannitol/fructose-specific PTS protein IIA from 9799-9353 on contig 135

[00214] SEQ ID NO: 82 is the ORF of encoding A. succinogenes 1-phosphofructokinase (EC 2.7.1.56) from 6480-5539 on contig 106

[00215] SEQ ID NO: 83 is the ORF of encoding A. succinogenes 1-phosphofructokinase (EC 2.7.1.56) from 6480-5539 on contig 54

[00216] SEQ ID NO: 84 is the ORF of encoding A. succinogenes fructokinase from 10269-11198 on contig 168

(e) General

[00217] SEQ ID NO: 85 is the ORF of the encoding A. succinogenes phosphoenolpyruvate phosphotransferase system enzyme I (EI) from 2202-475 on contig 153

[00218] SEQ ID NO: 86 is the ORF of the encoding A. succinogenes phosphotransferase system, HPr-related protein from 2565-2308 on contig 153

[00219] SEQ ID NO: 87 is the ORF encoding the A. succinogenes proton/sugar symporter from 41787-40432 on contig 165

(f) Sucrose

[00220] SEQ ID NO: 88 is the ORF encoding A. succinogenes sucrose invertase from 11272-12720 on contig 168

(g) Sugar outflow

[00221] SEQ ID NO: 89 is the ORF of the coding A. succinogenes potential main facilitation superfamily carbohydrate permease from 25395-26780 on contig 145

[00222] SEQ ID NO: 90 is the ORF of the coding A. succinogenes major facilitation superfamily sugar permease from 16827-15997 on contig 123

(iv) Dicarboxylic acid transporter

[00223] SEQ ID NO: 91 is the ORF of the coding A. succinogenes TRAP C4-dicarboxylic acid transport (Dct) system protein from 18087-16792 on contig 154

[00224] SEQ ID NO: 92 is the ORF encoding the large subunit of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 20342-19050 on contig 159

[00225] SEQ ID NO: 93 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 7334-6057 on contig 117

[00226] SEQ ID NO: 94 is the ORF encoding the large protein component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 13540-12269 on contig 122

[00227] SEQ ID NO: 95 is the ORF encoding the periplasmic component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system (dicarboxylic acid-binding protein) from 27696-26689 on contig 148

[00228] SEQ ID NO: 96 is the ORF encoding the periplasmic component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system (dicarboxylic acid-binding protein) from 21876-20890 on contig 159

[00229] SEQ ID NO: 97 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 14033-13551 on contig 12

[00230] SEQ ID NO: 98 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 20836-20345 on contig 159

[00231] SEQ ID NO: 99 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 18563-18087 on contig 154

[00232] SEQ ID NO: 100 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 26666-26157 on contig 148

[00233] SEQ ID NO: 101 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 12484-12975 on contig148

[00234] SEQ ID NO: 102 is the ORF of the small permease component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 7813-7331 on contig 117

[00235] SEQ ID NO: 103 is the ORF encoding the periplasmic component of the A. succinogenes TRAP-type C4-dicarboxylic acid transporter system (dicarboxylic acid-binding protein) from 19646-18669 on contig 154

[00236] SEQ ID NO: 104 is the ORF encoding the periplasmic component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system (dicarboxylic acid-binding protein) from 12245-11259 on contig 148

[00237] ORF encoding A. succinogenes TRAP-type C4-dicarboxylic acid transport system periplasmic component (dicarboxylic acid-binding protein) from 15018-14053 on SEQ ID NO: 105 is contig 122

[00238] ORF encoding A. succinogenes TRAP-type C4-dicarboxylic acid transport system periplasmic component from 5984-4998 on SEQ ID NO: 106 is contig 117

[00239] SEQ ID NO: 107 is the ORF encoding the periplasmic component of the A. succinogenes TRAP-dicarboxylic acid transport system from 4938-3949 on contig 117

[00240] SEQ ID NO: 108 is the ORF encoding an integral membrane component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 4284-3718 on contig 77. Note: The N-terminal coding region is missing because it extends beyond the edge of the contig.

[00241] SEQ ID NO: 109 is the ORF encoding the bulk membrane component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 26147-24867 on contig 148.

[00242] SEQ ID NO: 110 is the ORF encoding the integral membrane component of the A. succinogenes TRAP-type C4-dicarboxylic acid transport system from 12987-14288 on contig 148

[00243] SEQ ID NO: 111 is the ORF encoding A. succinogenes TRAP-type C4-dicarboxylic acid transport system components from 18563-18087 on contig 154

(v) Glycine synthesis

[00244] SEQ ID NO: 112 is the ORF encoding A. succinogenes serine deaminase or threonine ammonia lyase (EC 4.3.1.19), present on contig 141 from 10270-11808.

[00245] SEQ ID NO: 113 is the ORF encoding A. succinogenes glycine hydroxymethyltransferase (EC 2.1.2.1) from 2950-1682 on contig 137.

Entner-Doudoroff pathway genes:

[00246] SEQ ID NO: 114 is the ORF encoding A. succinogenes 2-keto-3-deoxy-6-phosphogluconate aldolase (EC 4.2.1.14) from 19472-18834 on contig 148.

[00247] SEQ ID NO: 115 is the ORF encoding A. succinogenes 2-keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14) from 15090-14452 on contig 159.

Transhydrogenase:

[00248] SEQ ID NO: 116 is the ORF encoding A. succinogenes NAD/NADP transhydrogenase alpha subunit from 24659-26191 on contig 152

[00249] SEQ ID NO: 117 is the ORF of the encoding A. succinogenes NAD/NADP transhydrogenase β subunit from 26203-27654 on contig 152.

[00250] SEQ ID NO: 118 is the ORF encoding A. succinogenes NADH dehydrogenase from 11481-10150 on contig 107.

[00251] While the invention has been described with reference to the illustrated embodiments, it should be understood that the invention is not limited thereto. A skilled artisan with ordinary skill in the art and having the teachings herein will recognize additional modifications and implementations that are within the scope of the invention. Accordingly, the invention is limited only by the appended claims.

sequence listing

<110>MICHIGAN STATE UNIVERSITY

<120> Genes from ACTINOBACILLUS SUCCINOGENES 130Z (ATCC 55618) for production of chemical products from A. SUCCINOGENES C4-pathway

<130>MSU 4.2-775 PCT

<140>PCT/US2006/030425

<141>2006-08-04

<150>US 60/705,841

<151>2005-08-05

<160>119

<170>PatentIn version 3.3

<210>1

<211>2048

<212>DNA

<213> Actinobacillus succinogenes

<400>1

ttaatttctt taatcgggac gctatcgata aattgaaaat gcagcaatag aggaaacacg 60

gtttgtttga gtgaaaacag ccgtgttttt tcatttaccg ccataaaaat ttgaaacgga 120

tcacaaatca tgaaaaaaat acgttcaaat tagaactaat tatcgaaaat ttgatctagt 180

taacattttt taggtataaa tagttttaaa atagatctag tttggatttt taattttaaa 240

ttatcaatga ggtgaagtat gactgactta aacaaactcg ttaaagaact taatgactta 300

gggcttaccg atgttaagga aattgtgtat aacccgagtt atgaacaact tttcgaggaa 360

gaaaccaaac cgggtttgga gggtttcgat aaagggacgt taaccacgct tggcgcggtt 420

gccgtcgata cggggatttt taccggtcgt tcaccgaaag ataaatatat cgtttgcgat 480

gaaactacga aagacaccgt ttggtggaac agcgaagcgg cgaaaaacga taacaaaccg 540

atgacgcaag aaacttggaa aagtttgaga gaattagtgg cgaaacaact ttccggtaaa 600

cgtttatcg tggtagaagg ttactgcggc gccagtgaaa aacaccgtat cggtgtgcgt 660

atggttatg aagtggcatg gcaggcgcat tttgtgaaaa acatgtttat ccgaccgacc 720

gatgaagagt tgaaaaattt caaagcggat tttaccgtgt taaacggtgc taaatgtact 780

aatccgaact ggaaagaaca aggtttgaac agtgaaaact ttgtcgcttt caatattacc 840

gaaggtattc agcttatcgg cggtacttgg tacggcggtg aaatgaaaaa aggtatgttc 900

tcaatgatga actacttcct gccgttaaaa ggtgtggctt ccatgcactg ttccgccaac 960

gtaggtaaag acggtgacgt ggctattttc ttcggtttat ccggtacggg taaaacaacg 1020

ctttcgaccg atcctaaacg ccaattaatc ggtgatgacg aacacggttg ggatgaatcc 1080

ggcgtattta actttgaagg cggttgttac gcgaaaacca ttaacttatc tcaagaaaac 1140

gaaccggata tttacggcgc aatccgtcgt gacgcattta tagaaaacgt cgtggttcgt 1200

gcagacggtt ccgttgactt tgacgacggt tcaaaaacag aaaatacccg tgtttcatat 1260

ccgattacc acatcgacaa catcgttcgt ccggtatcga aagccggtca tgcaaccaaa 1320

gtgattttct taaccgcgga cgcattcggc gtattgccgc cggtttcaaa actgactccg 1380

gaacaaaccg aatactactt cttatccggc tttactgcaa aattagcggg tacggaacgc 1440

ggcgtaaccg aaccgactcc gacattctcg gcctgtttcg gtgcggcatt cttaagcctg 1500

catccgattc aatatgcgga cgtgttggtc gaacgcatga aagcctccgg tgcggaagct 1560

tatttggtga acaccggttg gaacggcacg ggtaaacgta tttcaatcaa agatacccgc 1620

ggtattatcg atgcgatttt ggacggttca atcgaaaaag cggaaatggg cgaattgcca 1680

atctttaatt tagcgattcc taaagcatta ccgggtgttg atcctgctat tttggatccg 1740

cgcgatactt acgcagacaa agcgcaatgg caagttaaag cggaagattt ggcaaaccgt 1800

ttcgtgaaaa actttgtgaa atatacggcg aatccggaag cggctaaatt agttggcgcc 1860

ggtccaaaag cataaaactg taaaagcata gtatgtgcat gattcggtaa actaccgaat 1920

aaaatctgaa aaataaggct gagtatttcc actcagcctt ttgttttgga aattagaagt 1980

tgtttagaaa gataaacggc gcggcttatt cgtcgtatta tttgggacat aaaaaacccg 2040

ttgataaa 2048

<210>2

<211>939

<212>DNA

<213> Actinobacillus succinogenes

<400>2

atgaaagtaa ccttattagg cgccagcggc ggtatcggtc aacctctttc attgttgtta 60

aaattacatc ttccggcaga aagcgattta agcttatacg atgttgcgcc ggtcaccccc 120

ggtgtggcga aagacatcag ccatattccg acttcggttg aagtggaagg tttcggcggc 180

gatgatccgt ccgaggcatt aaaaggggcg gatatcgttt taatctgtgc gggtgtggcg 240

cgtaagccgg gtatgactcg tgcggatttg tttaatgtta acgccggtat tatccagaat 300

ttagtggaaa aagttgcgca agtttgcccg caggcttgtg tttgcattat cactaatccg 360

gtgaactcga ttattccgat tgcggcggaa gtgctgaaaa aagcgggcgt atacgataaa 420

cggaaattat tcggtattac tacgctggat accatccgtt ccgaaaaatt tatcgtgcaa 480

gcgaaaaata ttgaaatcaa ccgtaacgat atttcagtta tcggcggaca ttcaggtgtg 540

acgattttac ctttgttgtc acaaattccg catgtggaat ttaccgagca ggaattaaaa 600

gatttaactc accgcatcca aaatgccggc accgaagtgg tagaagctaa agccggtgcg 660

ggttccgcta cactttccat ggcgtatgcg gcaatgcgtt ttgtggtttc catggctcgc 720

gcattaaacg gcgaagtgat tacggaatgc gcctatattg aaggcgacgg taaattcgcc 780

cgtttctttg cacaaccggt tcgtttgggt aaaaacggcg tagaagaaat tctgccgtta 840

ggtacattaa gcgcatttga gcaacaagcg cttgaagcga tgttaccgac cttgcaaact 900

gacattgata acggtgtgaa atttgttacc ggcgaataa 939

<210>3

<211>1242

<212>DNA

<213> Actinobacillus succinogenes

<400>3

atgaaagtaa ccttattagg cgccagcggc ggtatcggtc aacctctttc attgttgtta 60

aaattacatc ttccggcaga aagcgattta agcttatacg atgttgcgcc ggtcaccccc 120

ggtgtggcga aagacatcag ccatattccg acttcggttg aagtggaagg tttcggcggc 180

gatgatccgt ccgaggcatt aaaaggggcg gatatcgttt taatctgtgc gggtgtggcg 240

cgtaagccgg gtatgactcg tgcggatttg tttaatgtta acgccggtat tatccagaat 300

ttagtggaaa aagttgcgca agtttgcccg caggcttgtg tttgcattat cactaatccg 360

gtgaactcga ttattccgat tgcggcggaa gtgctgaaaa aagcgggcgt atacgataaa 420

cggaaattat tcggtattac tacgctggat accatccgtt ccgaaaaatt tatcgtgcaa 480

gcgaaaaata ttgaaatcaa ccgtaacgat atttcagtta tcggcggaca ttcaggtgtg 540

acgattttac ctttgttgtc acaaattccg catgtggaat ttaccgagca ggaattaaaa 600

gatttaactc accgcatcca aaatgccggc accgaagtgg tagaagctaa agccggtgcg 660

ggttccgcta cactttccat ggcgtatgcg gcaatgcgtt ttgtggtttc catggctcgc 720

gcattaaacg gcgaagtgat tacggaatgc gcctatattg aaggcgacgg taaattcgcc 780

cgtttctttg cacaaccggt tcgtttgggt aaaaacggcg tagaagaaat tctgccgtta 840

ggtacattaa gcgcatttga gcaacaagcg cttgaagcga tgttaccgac cttgcaaact 900

gacattgata acggtgtgaa atttgttacc ggcgaataat tcaccaaaat aatttaacaa 960

aaccgattaa aggattaggt ttttatgcaa acctaatcct ttttgtttgg tatcaatcag 1020

ttaaaatccg ccgtttgatt aatgggaagc tatataagat tttagtattt tatatagata 1080

aaaatagcgt ggaagaataa agtaatcctc cacgcgtctt ctcaaatgta taaaaagtgc 1140

ggtcaaaaat taatcgattt tttatcatc ctcgtttctt ggcggtttaa tcgccagtaa 1200

attacatttt aacttactga taacatgttc ggcggtgttg ct 1242

<210>4

<211>1395

<212>DNA

<213> Actinobacillus succinogenes

<400>4

atgacatttc gtattgaaaa agacactatg ggcgaggttc aagttcctgc ggataaatat 60

tgggccgcac aaacggaacg ttcacgcaat aactttaaaa tcggacctgc agcgtcaatg 120

ccaacacgaaa ttattgaagc attcggttat ttgaaaaaag cggccgctta tgccaacgcg 180

gatttaggcg tattgccggc tgaaaaacgc gatttgatcg cacaagcctg cgatgaaatt 240

ctggctcgta aattagatga tcaattcccg ttagttatct ggcaaaccgg ttcgggtaca 300

caatccaata tgaatctgaa cgaagttatc gctaaccgcg cacatgtgat taacggtggt 360

aaattaggtg aaaaatctat tattcatcca aacgacgatg taaacaaatc tcaatcttca 420

aacgatactt atccgacagc aatgcacatt gccacatata agaaagtggt tgaagcaacg 480

attccggcca tcgaacgttt acaaaaaacc ttagcggcga aatccgaaga attcaaagat 540

gtggtgaaaa tcggccgtac gcacttaatg gatgccaccc cgttgacatt gggtcaggaa 600

ttcagcggtt atgctgcaca attaagtttc ggtttagcgg caatcaaaaa taccttaccg 660

catttacgcc aactggcatt aggcggtacg gcagtgggta ccggtttaaa tacacctaaa 720

ggctatgatg taaaagtagc ggaatatatc gccaaattca ccggcttgcc gtttattacc 780

gccgaaaaca aatttgaagc attagcaaca catgacgcta tcgttgaaac tcacggcgca 840

ttaaaacaag ttgcgatgtc cttattcaaa attgcaaatg atatccgttt attggcttca 900

ggtcctcgtt ctggtatcgg tgaaatttta attcctgaaa acgaaccggg ttcatccatc 960

atgccgggta aagttaatcc gacccaatgc gaagcgatga caatggttgc cgcacaagta 1020

ttaggtaacg ataccactat ttcatttgcc ggttcgcaag gtcatttcga attgaacgta 1080

ttcaaaccgg ttatggcggc aaatttcctg caatccgctc aattaatcgc agatgtttgc 1140

atttctttcg acgagcactg tgcaagcggc attaaaccaa atacgccgcg cattcaacac 1200

ttacttgaaa gttcattaat gttagtgacc gcattaaata ctcatatcgg ttatgaaaat 1260

gcggcgaaaa ttgcgaaaac tgcgcacaaa aacggtacaa cattacgtga agaggctatc 1320

aacttaggtt taggtccgc cgaagatttc gataaatggg ttcgtccgga agatatggtt 1380

ggcagcttaa aataa 1395

<210>5

<211>1791

<212>DNA

<213> Actinobacillus succinogenes

<400>5

gtgcaaacag tcaatgtcga tattgcgatt gtgggtgccg gcggcggcgg tttacgtgcg 60

gcgattgcag cagcagaagc gaaccctaac ctaaaaattg cattagtttc aaaagtttat 120

ccgatgcgta gccataccgt ggcggccgaa ggcggtgcag cggcggttat caaagaagaa 180

gtagttatg acaaacactt ccaagacacc gttgcgggcg gtgactggtt gtgcgaacaa 240

gacgttgtag aatatttcgt taaacattca ccggtggaaa tgacgcaact cgaacgctgg 300

ggttgtcctt ggtcgcgtaa agcagacggt gatgtaaacg tacgccgttt cggcggaatg 360

aaaatcgaac gtacttggtt cgctgccgat aaaaccggct tccatatgct tcatacgtta 420

ttccaaacct ctattcaata tccgcaaatt caacgtttcg acgaacactt tgtgttagat 480

attttagtgg acgacggcca tgcccgcggg atggttgcca tgaatatgat ggaaggcaca 540

ttggtgcaaa ttaacgctaa cgccgttgtt atcgcaaccg gcggcggttg tcgttcattt 600

aaattcaaca ccaacggcgg tatcgtaacc ggtgacggtt tatctatggc gtatcgtcac 660

ggcgtaccgc ttcgcgacat ggaattcgtt caataccacc cgaccggctt gccgaatacc 720

ggtatcttaa tgaccgaagg ttgtcgtggt gaaggcggta tcttagtcaa taaagacggt 780

tatcgttatt tacaggatta cggtttagga cctgaaacgc caatcggcaa accgcaaaac 840

aaatacatgg aattggggcc tcgcgataaa gtttcccaag cattctggca ggaatggaaa 900

aaaggcaata cactgaaaac cgcaaaaggc gtggatgtgg tgcatctaga tttacgtcac 960

ttgggtgccg attacktaca cgaacgtctt ccgttcattt gtgaattagc taacgcttat 1020

gaaggtgtgg atccggttaa agaacctatt ccggttcgcc ctgtggtgca ctataccatg 1080

ggcggtattg aaatcgactt caatagcgaa acccgcatca aaggtttatt tgcagtaggc 1140

gaatgtgcgt cttcgggttt acacggcgca aaccgtttag gttccaactc tttagcggaa 1200

ttagtggtat taggccgcgt tgccggcgaa catgccgcac aacgtgcaac ggaagctcaa 1260

tccgttaacc aagcggcggt ggacgctcaa gcacgtgacg tagtgaaacg tctggaagat 1320

ctttacaatc aggaaggtac tgaaaactgg gcggatatcc gcgaagaaat gggtaccgcg 1380

atggaagaag gttgcggtat ttatcgtgac gaagccagta tgcaaactgc agtcaataaa 1440

attgccgaat taaaagaacg ttataaacgc atccgcattc aagaccgttc aagcgtgttc 1500

aacaccaatg tcctttacac tgtggaatta ggttacatct tagatgttgc tcaatctatc 1560

gctaattccg cattacaacg taaagaatcc cgtggtgcgc accaacgttt agattacaca 1620

gaacgtgatg atgtgaatta tttaaaacat acgcttgctt tctacaacgg caacgatgca 1680

cctcgtatcg attacagcga tgtgaaaatt accacattcc aaccagctaa acgcgtatac 1740

ggtgcggaag ccgaagctca agagaaagcg gctaaagccc ctaacgcata a 1791

<210>6

<211>75

<212>DNA

<213> Actinobacillus succinogenes

<400>6

ttggaaccgg agctttccta ccgttggtct tgccgcatgg caatctgcgg ttcttgcggt 60

atagatggta aataa 75

<210>7

<211>582

<212>DNA

<213> Actinobacillus succinogenes

<220>

<221>misc_feature

<222>(1)..(11)

<223> Region overlapping with iron-sulfur protein ORF

<220>

<221>misc_feature

<222>(281)..(281)

<223> n is a, c, g, or t

<400>7

atggtaaata attcctaa attggcatgt aaaactttct tgcgcgatta cagcggtcat 60

atgcgtatcg aagcattggc gaatttccct atcgaacgcg atttagtggt agatttaagc 120

catttcatcg aaagtttgga agcaattaac ccgtacatca tcggtaataa aatgcctgac 180

ctggacggtc aaccgcacga atcggcggaa ttacaaaaaa gccgaacaaa acaaaccccg 240

aaacagttgg aaaaatcccg tcgattctca atgtgtatca nctgcggttt gtgttatgcg 300

gcctgtccgc aattcggttt aacccctgaa ttcgtcagac ctgccgtgta cactatggct 360

ccccgctata acttggatac gcgcgaacac ggcaaagccg aacgtatgcc gattatcaac 420

ggtgacaatg gtgtatggcc ctgtactttc gtaggtgcat gttctgaagt ctgtccgaaa 480

catgtaaacc cggctgcagc aattaaccaa ggcaagctgg aaagtgcgaa agacttttta 540

atctcaatgg ttaaaaccaa aagcataggg ggaaagcaat ga 582

<210>8

<211>390

<212>DNA

<213> Actinobacillus succinogenes

<220>

<221>misc_feature

<222>(1)..(4)

<223> Region overlapping with the C-terminus of the frdB product

<400>8

atgagcgaat taaccacaaa acgtaataaa tacgtccgtg aaatcacccc gacttggtgg 60

aaaagctggg atttctatcg tttctacatg gtacgtgaag cgacagccgt tccgactgtt 120

tggttctgtt tagtattgct ttacggcgta ttctcactcg ccgataatac ctttgctact 180

gactttgtcg gttttatgca gaatccgatt gtgatgatat taaatatcat ctcattggca 240

gcaatgttat tacatgcttt tacgctgttt accatgactg gtgaagtaat gtccaacagc 300

accggtttaa aagcggaagt tatcaaaaag gcattgcaaa ttgctttcgc cgttgtttcc 360

gtcatcggct taattcttgc cttcatttaa 390

<210>9

<211>345

<212>DNA

<213> Actinobacillus succinogenes

<400>9

atgatcaata caaatccgaa acgttctaac gagccacctg tatggttat gttcagtgca 60

ggcggcatga tcagcgcact ggcttttccg gttattaatcc taatcttagg gattttactg 120

cctttaggta ttatttcacc ggatggcatt attgctttcg cccatcactg gtttggtaaa 180

ttagtgattt tagtgctaac tatttttccg gcctgggcgg gtttacaccg catccaccac 240

ggtatgcacg atattaaagt acacgtgccg tccggcggtt taatcttcta cggattagcg 300

gtactttata ctgtcgttgc aatttggggc gttgcgtcaa tctaa 345

<210>10

<211>1161

<212>DNA

<213> Actinobacillus succinogenes

<400>10

atgaatctac acgaatacca atcaaaacaa atttttgcgc agtacgattt gcccgtcagc 60

aaaggttatc cgtgcgaaac ggtggagcag gcattgaaag cgattgaaaa actgggcggc 120

gaccaatggg tggcgaaatg tcaggtatat gccggaggtc gcggcaaagc gggcggcgta 180

aaagtggtga gttccgaagc ggaagtgcgg tcgttttttg aacggttttt aggtaaacgc 240

ttggtgacat tgcaaacgga tgctcagggt cagccggtga ataccatta tctggaggcc 300

tgcgccgcag taaaaaaaga attgtatgtg ggaatggtga ttgatcgcgc caaccgccgt 360

atcgtattta tggcgtcaac ggaaggcggc gtaaatatcg aagaagtcgc ggaaaaaaca 420

ccgcatttac tgcataaagt agcgatagac ccgttcatgg gcgcacaacc ttttcagggg 480

cgcgaactgg cctgtaaatt agggttgacc ggtaaacaaa tccatcaatt tgcccatatt 540

ttctgccggt tgagcaaaat gttcagcgaa aaagatttga gtttggtgga aatcaatccg 600

ttggtgattt tgcaaaatga cgatctgcat tgtctggatg ccaaaattgt ggtggacggt 660

aacgccttat atcgccatgc cgatttgaaa tccttgcagg atccgagtca ggaagatccg 720

cgcgaagcgg aagcggaagc cttggggcta aattatgtgg cgttggacgg caatatcggt 780

tgcatggtga acggcgcagg tttggctatg ggtactatgg acatcataaa actgcaggga 840

ggattgccgg caaactttct tgacgtaggc ggcagcgcta ccaaagaacg ggtggcgggc 900

gcgttcaaaa tcattctttc cgacaccaat gtaaaagcca ttttggtgaa tatttttggc 960

ggcattgtgc gttgcgatct tatcgccgaa ggtattgtgg cggcagtcaa tgaagtgggc 1020

gttaccgtgc cggtggtggt gcgcttggaa ggtaataacg ccgaacgcgg acgggaaatt 1080

ttggctcaaa gcggacttaa cattattgcc gcagaaagct taaaagatgc agctgttcag 1140

gctgtgaacg cagcaaaata g 1161

<210>11

<211>56

<212>DNA

<213> Actinobacillus succinogenes

<400>11

attttaatta ataaaaacac taaagtgatc tgccagggat tcaccggcgg acaagg 56

<210>12

<211>260

<212>DNA

<213> Actinobacillus succinogenes

<400>12

attttaatta ataaaaacac taaagtgatc tgccagggat tcaccggcgg acaaggcacc 60

ttccacagcg aacaggcatt ggcatacggc acgaaattag tgggcggtac cagtccgggt 120

aaaggcggta cgacgcattt gggattgccg gtgtttgata cggtacgcga agcggtgcaa 180

aaaaccggag ccaccgccag cgtgattat gtgccggcac cgttctgtaa ggacgccatt 240

ctggaagcga ttgacgccgg 260

<210>13

<211>1428

<212>DNA

<213> Actinobacillus succinogenes

<400>13

atgaaaaacg tacgtgttga agtggattta ttaggcgaac gcgaagtatc gaatgatgtg 60

tattggggga ttcatacatt aagagcggtg gaaaatttca atatttccga tcattgcatt 120

tccgacgtac cggaattcgt acgcggtatg gtgatggtga aaaaagcgac ggcgatggcc 180

aacggcgaat taggtgcgat tccgaaaaaa gtctccaaag ctatcgttca ggcttgtgat 240

gaaatcctgt tgaacggacg ttgcatggat cagttcccgt ccgatgttta tcaaggcggc 300

gcaggtactt ccgtgaatat gaataccaac gaagtcgtag ccaatttagc cttagaaatt 360

ttaggtcatc aaaaaggcga atacgatatt atcgacccga tggatcacgt gaacgccagc 420

cagtctacca acgatgccta cccgaccgga ttccgtatcg ccgtgtacaa cagtattatg 480

catctgattg aaaaaatcga atatttgcaa caaggtttcg acaaaaaagc ggaagaattc 540

gccaatgtgt tgaaaatggg gcgtactcag ttacaagacg ccgtaccgat gaccgtggga 600

caagaattca aagcctttgc gattctgctt gaagaagaag cgaaaaattt aaaagatacg 660

gcggaattat tgctggaagt caacttaggt gcgacggcaa tcggaaccgg tttgaatacc 720

ccggaaggtt accgcgaatt ggcggtgaaa tgtttatccg aagtaaccgg tttgccttgc 780

gtcggttccg aaaatctgat tgaagccact tccgactgcg gttcttatgt aatggtgcac 840

ggcgcgctga aacgaagcgc ggtcaaaatt tcgaaagttt gtaacgactt acgtctgctg 900

tcatccggtc cgcgagcagg attgaacgag atcaatctgc ccgaattgca agccggttct 960

tccatcatgc cggcaaaagt aaatccggtt gtgcccgaag tggtaaacca agtgtgcttt 1020

aaagtgatcg gtaacgatac gaccgtaact tttgccgccg aagccggcca gttacagtta 1080

aacgtaatgg aaccggtaat cggtcaagcg atgttcgaat ctatcgatat tctcgctaac 1140

gcatgtgtga atttgcgtga taaatgtatc gacggtatca ccgtaaacaa agaaatctgt 1200

gaaaattacg tgtttaactc tatcggtatc gtgacctatc tgaatccgtt tatcggccac 1260

cataacggcg atattgtggg taaaatctgc gcacaaaccg gtcgcagcgt acgagatgta 1320

gtactggaaa aaggcttact gaccgaggcg cagttagatg atattttatc cgttgaaaac 1380

ctgatgaaac cgacctataa agcggcgcgt tttaccgacg aagaataa 1428

<210>14

<211>1119

<212>DNA

<213> Actinobacillus succinogenes

<400>14

gtgaaatggc ataaagcggt aggcgacgac attaaacgcg atgacattct cgtggaagtg 60

gaaacggata aagtggtttt ggaagtgccg gcgttggaca gcggcgtact ggaaagcatt 120

gaccagccgg aaggtgcgac ggttgtcagc aaacaactat tagggcgtgt agcaaaaggc 180

gaaaccgtgg tagcaccggt ttctgcccaa ccgttacaaa ccgaatcccg tccggcaccg 240

gatcatcatg attcgctcgg accttccgtg cgccgtttgg tgggcgaaca cgatttaaat 300

ccgcaggata tttccggttc cggcaaacac ggtcgtatca cgcgtgaaga cgtagcgcag 360

gctttggcgg ataaagaagc gcaatcagcc caaactgcgg tcaaaaactc agcggaattt 420

cgcgatgagc agcgcgtgcc gatgacgcgt ctgcgtaaac gcatcgccga acggttatg 480

gaagtgaaaa ataccacggc aatgctgact acctttaatg aggtggatat gcagccgatt 540

atgcagttgc gcaaaaagta cggtgaaaaa tttgaaaaac agcatgatgt gcgcctcgga 600

tttatgtctt tctacgtgaa agccgtggta gaagccttga aacgttatcc ggttatcaat 660

gctaccatcg acggcgacga tattctgtat cacaattatt tcgatatcag tatcgccgtt 720

tccaccccgc gcggcttggt gacgccggtg ttgcgcgatt gcgataaaat gagtatggcg 780

gatatagaga aacaaatcaa agtgttggcg gaaaaaggtc gcgacggcaa actcacggtg 840

gaagacttaa ccggcggcaa tttcactatt accaacggcg gggtattcgg ttcgttaatg 900

tccaccccga ttatcaatcc gccgcaaagc gctattttgg gcatgcacac tattaaagac 960

cgtccggttg cagtagacgg ccaagtagtg attcgcccga tgatgtattt ggcattatct 1020

tatgatcacc gcttaatcga cggcaaagac agcgttcgat ttctggtcac cattaaagat 1080

ttgctggagg atccgaccag actgttattg gaaatttaa 1119

<210>15

<211>2826

<212>DNA

<213> Actinobacillus succinogenes

<400>15

atgccgaata atcagtttca ttcatttcag caatggctca gtcaaacagc gctgggcggc 60

gcgaatcaat cttatatcga agccctttac gaagattacc tgcaagatcc gaataacgtc 120

gacgacagct ggcgcaccgt ttttaattct ttcccgaaac ccaccgcgct ttcgccggaa 180

caacccccatt ctccggtacg cgattatttc aagcgccttg cccgcgataa tactcgggcc 240

ggcacaaccg ttgtggtgga tccgaaagtc agcgcgcgtt tagtcaaggt attgcaatgg 300

gtgaacggac atcgcaaccg cgggcactta gatgcgaatc tggatccgtt aaaactttgg 360

gaacgtatgc cgtctccgac tttggattat aagttttacg gttttaccga tgcggatttg 420

gataaagaat tcgatattgg cggttatgtg tataaccgtg aaaaaatcac cttgcgcgaa 480

ttggaatata atctgaaaaa cacctactgc ggcactttgg gtttcgagtt catgcacgtg 540

aacgatttgg aggcgcgtac atggttacag cgcaaagcgg aaaatttact gaataagccg 600

ctgtttaatc gtgacgagaa gatacggttt ttagaagaat taaccgccgc ggacggcctg 660

gaacgttact tgggcgcaaa atttccgggg gcaaaacgtt tttcgctgga gggcagtgac 720

ggttatatcc cgttaatgaa agagattatt cgccacggcg gtaaaaacgg cgtgaaagaa 780

atcgtattcg gcatggcgca ccgcggacgg ttgaacatgc tggtaaatgt gctgggtaaa 840

gaaccgtcgc agctgtttga tgaatttgcc ggcaaacatt ccggcgatcg caccggcgat 900

gtgaaatacc atcagggcta ttcttccgat tttatgacgg aaggcggcgt agtgcatttg 960

gctttggcct ttaacccgtc tcatttggaa attgtcagtc cggttgtcat cggttctgtg 1020

cgagcccgcc aaaaacggtt gggtgatcaa accaaagatc aggttttacc tgtcaccgtg 1080

catggcgatt ccgccgtaat cggacaaggc gtagttcagg aaaccttaaa catgtcggca 1140

acccgaggtt ataacgtggg cggtactatt cgtgtggtaa ttaataacca aataggtttc 1200

accacctcca ataccaaaga tacccgctcc acggaatact gtaccgatat cgctaaaatg 1260

atcgaagcgc cggtaattca tgtgaacggc gacgatcctg aagccatcgc ttatgcggca 1320

cgtatggcgg tggaattccg catgcggttt aaacgggata tttttatcga tctggtgtct 1380

taccgtcgcc atggccacaa tgaggcggac gaacccttag ccactcaacc ggtgatgtat 1440

aaactcatta aaaaacatcc gacagcgcgt aaagtatatg cggatcggct ggtgagcgaa 1500

ggcgtcatta acgaatccga cgctatcgaa ttgatgaatg attaccgtaa ggctttggat 1560

gaaggtgatt gtgtggtgaa agaatggcgt aaaacgaata aaccttcggc acaatggtat 1620

aaattccttg gcgcggataa cggcggacgg gaagcggaat acaacggtcg gtttgatccg 1680

gttcgtttta aaactttagc ggaacgtgtt tgcgaatatc cggctgacca tccgattcat 1740

ccgcgagtgg aaaaggttta taacgaccgt aaattaatgg ctgcgggtga aaaagcgctg 1800

gattggggaa tggcggaaat tatggcgtac gctaccttgc tggcggacgg tcatcatgta 1860

cgtatttccg gtgaggacgc cggacgcggg actttcttcc atcgtcaggc ggtgttgcat 1920

aatcaaaacg agcgcaaagc ctatattccg ttgatgcatt tgcagccgca acaaggacgc 1980

tttgaagtgt gggattccgt actttccgaa gaagcggtgt tagcgtttga atacgggtat 2040

gcggcgacgg atcccaatac gctgaccatt tgggaggcgc aattcggcga tttcgccaac 2100

ggtgcgcagg tggtgatcga ccagtttatc agttccggcg aacaaaaatg gggcagaatg 2160

tgcggtttgg tcatgttact gccgcacggt tacgaaggac aggggccgga attcctcc 2220

gcccgtctgg agcgttattt acaactttgc gcccagcaaa atatgcaggt ttgcgtgcct 2280

tccacgccaa gtcagattta ccacttattg cgccgtcaaa tgttgcgcgc cgagcgtcgt 2340

ccgttaatcg tgatatcgcc gaaatcttta ttacgccacc cgctggtggt gtcgcaaacg 2400

gacgaattcg ttaacggcgc tttccgtcgc gtgattgacg aaatcgacga cattaaaccg 2460

gaaaatgtca agcgggtgat tttctgcgcc ggtaaagtgt attacgattt attggaagag 2520

cgtcgtaaca aacaacaaac cgacatcgcg attatccgta tcgaacagct ttatccgtat 2580

ccgcacgaag aagtgcggtc ggttttggca aagttttccc atgtgaagga tttcatctgg 2640

tgtcaggaag agccgcttaa ccaaggcgcg tggtattgca gccagcataa tttccgtgcc 2700

ggtttgccgg atggcgccac cctgaattac gtgggacgcc cggcttccgc ttcgccggcg 2760

gcaggctacg gcgcattgca cagcacacaa caacgtacat tgattacgga agcattaaca 2820

ttttaa 2826

<210>16

<211>1425

<212>DNA

<213> Actinobacillus succinogenes

<400>16

atgagcaaag aaattaaaac acaagttgtc gtgttgggtg caggtcctgc ggggtattcc 60

gccgcattcc gttgtgcgga cttaggattg gaaaccgtat tggtcgaacg ctattcaacg 120

cttggcggag tatgcttaaa cgtgggctgt attccatcca aagccttat gcacgtggca 180

aaagtaattg aagatgctaa acacgtcgaa caccacggcg tagtattcgg tgaaccgaca 240

atcgatttgg ataaaatccg agccggtaaa gacggcgtgg taggccgttt aaccggcggt 300

ttggccggta tggcaaaaat gcgtaaagtt accgtagtgg aaggcttggc aaaatttgca 360

gatagccaca ccctggtggc gacggatcgt gaaggtaatg cgactacaat taaatttgac 420

aatgcgatta ttgcggcggg ttctcgtccg attcaacttc cgtttattcc gcatgaggac 480

ccacgcgttt gggattctac ggatgccctc gcgttacgtg aagtaccgaa aaaattactc 540

gttatgggcg gtgggatcat cggtctggaa atgggaacgg tttacagtgc tttaggttcg 600

cagatagatg tggtggaaat gttcgatcaa gtaattcctg cggcggataa agacatcgtt 660

aagatcttta ctaaacgtat cgaaaataaa tttaacttat tgcttgaaac caaagtaact 720

gccgttgaag cgaaagaaga cggtattcat gtttccatgg aaggtaaagc ggcaaacgaa 780

acccgtgttt atgatgcagt attggtggct atcggccgta caccgaacgg taagctgata 840

gatgcggaaa aagccggagt agaagtggat gaacgcggct ttatccgtac cgataaacaa 900

atgcgcacga atgtgccgca catttatgct atcggcgaca ttgtcggaca accgatgtta 960

gcgcacaaag gcgtacatga aggtcatgtt gctgccgaag taattgcggg caaaaaacac 1020

tattttgatc ctaaagtaat cccttccatc gcttataccg aaccggaagt ggcttgggta 1080

ggtaaaaccg agaaagagtg caaagctgaa aatctgaatt atgaagtggc gacatttcca 1140

tgggcggcat ccggtcgtgc tatcgcctcc gattgtgctg acggtatgac gaaattgatt 1200

ttcgacaaag aatcgcaccg tattttaggt ggtgcgattg tcggtactaa tgccggcgaa 1260

ttactgggtg aaatcggttt agccatcgaa atgggttgcg atgcggagga tattgcctta 1320

actatccacg cccacccgac tttacacgaa tcggtcggtt tggcggcgga agtatttgaa 1380

ggctccgtta cggatttacc aaatgcgaaa gccaagaaaa aataa 1425

<210>17

<211>1350

<212>DNA

<213> Actinobacillus succinogenes

<400>17

atgtcaaact cggtagcatc attagaagaa tttttagctt tggtggcaaa acgcgacggt 60

aatcagccgg aatttttaca ggccgtacgt gaagtgttta catcaatctg gccgttcctg 120

gaaaaaaatc cgcaatatcg ctcacaagcc ttatggaac gcttggttga accggaacgc 180

gctatccagt tccgggtggc atggacggat gacaaaggtc agactcaggt taaccgagct 240

ttccgcgtgc aatttaacag tgcgatcggt ccgtttaaag gcggtatgcg tttccatccg 300

tccgttaact tatctattct gaaattctta ggcttcgaac aaatcttcaa aaacgcgtta 360

actacattac cgatgggcgg cggtaaaggc ggttccgact ttgatcctaa gggcaaatcc 420

gacggcgaag tcatgcgttt ctgccaagcg ttaatctcgg aattatatcg tcacgtgggt 480

ccggatacgg acgtaccggc gggtgatatc ggtgtaggcg gtcgtgaagt aggttatctt 540

gccggttata tgaaaaaatt atctaaccaa gccgcatgcg tgtttaccgg tcgcggtttg 600

tcattcggcg gcagcttgat tcgcccggaa gccacgggat acggtttggt ttacttcgca 660

caagcaatgt tggcggaaaa aggtcaatct ttcgacggca aaaccgtgct cgtgtccggt 720

tccggtaacg tagcgcaata cgctattgaa aaagcgcttc aattaggcgc gaaagtggtc 780

acttgctccg acagttccgg ttatgtttat gatgaagcgg gctttactgc ggaaaaatta 840

gccgcattta tggaaatcaa aaacgttcaa cgcggtcgtg taaaagatta tgcggaaaaa 900

ttcggcttaa aatatgttgc cggtgaaaaa ccgtggacgg taaaagccga tatcgcatta 960

ccatgcgcaa cgcaaaacga attggatttg gacgctgcaa aaaccttaat cgcaaacggt 1020

gtgcaattag tggcggaagg tgcgaatatg ccgacgacta ttgaagcgac cgaagcattg 1080

caacaagccg gggtgttgtt cggaccgggt aaagcggcca atgccggcgg tgtggcgact 1140

tcaggcctcg aaatggcgca gggttcgcag cgtgaatact ggacggccga agtagtcgac 1200

caaaaactgc ataacatcat gctggacatc catgccaact gtaaaaaata cggtaccgac 1260

gaacaaggga atatcaacta tgttgccggt gcaaacatcg ccggtttcgt aaaagtagcc 1320

gacgcgatgt tggcgcaagg cgtttactaa 1350

<210>18

<211>1437

<212>DNA

<213> Actinobacillus succinogenes

<400>18

atgtctagaa gattaagaag aacgaaaatc gtatgtacga tgggacctgc gaccgacaaa 60

ggcaacaacc ttgaaaaaat cattgccgca ggtgcaaacg tagtacgtat gaacttctcc 120

cacggaacgc cggaagatca tatcgcccgt gccgataaag tacgagaaat tgcgaaaaaa 180

ttaggcaaac acgtcgcaat cttgggtgat ttacaaggcc ctaaaatccg ggtttccact 240

ttcaaagaag gcaaaatttt cttaagtatc ggtgataaat ttatttaga tgcggattta 300

ccgaaaggtg aaggtaatca ggaagcggtc ggattggatt acaaaacttt accgcaggat 360

gtcgtgccgg gcgatatttt attgttagac gacggacgcg ttcagttaaa agtattatct 420

acggacggcg cgaaagtgtt taccgaagta acggtaggcg gtcctttgtc aaacaacaaa 480

ggtatcaata aattaggcgg cggtttatcg gcggacgcat taaccgagaa agataaagcg 540

gatatcgtta ccgcggcacg tatcggcgta gattatctgg cggtgtcttt cccgcgttca 600

agcgcagact taaattatgc ccgcgaattg gcggaagccg ccggcttaga tgcaaaaatc 660

gtagcgaaag tagaacgtgc ggaaaccgtg gcgactgacg aagctatgga cgatattatc 720

ttagcgtcgg acgttatcat ggttgcccgc ggtgacttgg gggtggaaat cggtgacccg 780

gaattagtgg gcgttcagaa aaaattaatc cgtcgttcac gtcagttaaa ccgagtggtt 840

atcactgcga ctcagatgat ggaatcgatg atcagcaacc cgatgcctac ccgtgcggaa 900

gtaatggacg tcgcgaatgc cgtgttggac ggtaccgatg ccgtaatgct ttcagcggaa 960

accgcagccg gtcaatatcc gtccgaaacg gtggcggcta tggcgaaagt ggcgttgggg 1020

gcagaaaaaa tgccgagtat caacgtatct aaacaccgta tggacggtca attcgccact 1080

atcgaagaat ccgtagcgat gtcttccatg tatgcggcaa accacatgaa aggtgtggcg 1140

gcgattatta ccttgaccaa cagcggccgt acggcgcgtt taatgtcgcg tatcagttcc 1200

ggtttaccga tttttgcctt atcgcgtaac gaatccactt taaacttatg cgcgttatac 1260

cgcggtgtaa cgccgattct gttcgaacag gaatcccgca ccgtacacag cgcgaaagcg 1320

gcggtcaatc tgttgaaaga aaaaggattc ttggtgtcgg gcgacttggt gttattaact 1380

cagggggacg cactaggcac atcaactaac gtatgccgca cattaatcgt tgaataa 1437

<210>19

<211>2436

<212>DNA

<213> Actinobacillus succinogenes

<400>19

aatttaaaaa ttgaacgccg cattcgttcc gctgtacgtt ggaatgcgat tatggcggtt 60

ctgcgcagtc agaaaaaaga tcttgatta ggcggccata tttctactta tcaagcggcc 120

gcaactatgt atgaggtttg ttttaaccat ttctttaaag cggctaccga gcaagacggc 180

ggtgatttag tatttttcca aggccatgcc gcaccgggta tgtatgcacg cgcattttta 240

gaaggccgta ttactgaaga gcaaatggat aatttccgtc aggaagcgtt tgccgacggt 300

ttatcttctt atcctcatcc gcgtttaatg cccgaatttt ggcagttttc taccgtttct 360

atgggatgg gcccggtaaa tgccattat caggctcgtt ttctgaaata tcttgccaat 420

cgcggtttga aagatacttc taaccaaaaa gtctatgctt tcttaggcga cggtgaaatg 480

gatgaaatcg aatctaaggg agcattagct tttgctgccc gtgaaaaatt agacaatttg 540

attttcacaa tcagctgtaa cctgcaacgt ttagacggtc cggtcgacgg taacggtaaa 600

atcgtacaag aattagaagg attatttacc ggtgcgggct gggaagtgat taaagtcatg 660

tggggcagca actgggatag attattcgct aaagatacct ccggtaaatt gactcagttg 720

atgatggagg ttctggacgg cgattactta acctttaaat ctaaagacgg tgcttacgtt 780

cgcgaacact tcttcggtcg ctaccctgaa accgcagcgt tagtggcgga tatgacagac 840

gatgaaatat gggcattacg ccgcggtgcg catgatgcgg aaaaaatgta tgcggcatat 900

gctaaagcgc aaactgccgg cagaccggtt gtcattttag ctcaccaagt gaaaggttat 960

aaaattcctg aagcgcaaag taaaaatacc gctcatcaat cgaaaaaaat gtctcaacaa 1020

agccttaaag gtttccgtga ctacttccag ttaccattaa cggatgagca ggttgaaaac 1080

ttagaatacg ttaaattccc tgaaggttct gacgaatata actacctgca cggtcaacgt 1140

aaagcgttaa acggttattt gccggcacgt cgagcaaaat tcgatactga gtttaaagtg 1200

cctgcattaa cggacttcgc ccagttgtta gacgcacaag cgcgcccgat ttcaacgact 1260

atggcctttg ttcgcgtatt gaatacttta ctgaaagata aaaatatcgg taaacaaatc 1320

gtaccgatta ttgcggatga agcgcgtact ttcggtatgg aaggtttatt ccgtcaaatc 1380

ggtatttata atccgcacgg acaaaattac gtaccttcgg accggggattt agtggcttat 1440

taccgagaag cgaaagacgg tcaggtattg caggaaggaa ttaacgaatt aggtgcgact 1500

tcatcttggg ttgccgccgc aacatcttat tccgtaaata atttgccgat gattccgttc 1560

ttcgtttatt actccatgtt cggtttccaa cgtgtaggcg atatgatgtg gttagccggc 1620

gaccaattag cccgcggctt ctttatcggc ggtacttccg gccgtacgac attgaacggc 1680

gaaggtttgc agcatgaaga tggtcacagc cacattcagt cgtcggtgat cccgaactgc 1740

gtatcttatg atccggcatt cgcgtttgag gttgcggtga ttatgcaaga cggtattaac 1800

cgtatgtacg gtgaaaaaca agaagacgtg ttctactaca tcaccacatt aaatgaaact 1860

tatgatcaac cggcaatgcc ggccggtgcc gaagagggta tccgtaaagg tatctacaaa 1920

tttgaaaccg tcggtaaagg cgaagcggca gttcaattaa tgggttccgg cgcaatctta 1980

cgccatgttc gtgaagcggc tcgaattttg gctgatgact acggtattac ttccgatgta 2040

ttcagcgtga cgtcatttac cgaagtcgcc cgtgaaggtg cggaagcggc tcgttggaat 2100

ttattacatc cgaccgaaca acaacgcgta ccttacattg cgcaagtgat gtccgataag 2160

ccggccgttg ccgcaaccga ctacatgaaa ctttatgcgg aacaggttcg tgcgtttat 2220

ccggcacaaa attatcacgt attaggtacc gatggttttg gtcgctcgga cagccgtgca 2280

aacttacgtg agtttttcga ggtggatgca cattatgtgg tgattgccgc actatcgcaa 2340

ttagcgaaac aaggcaaagt cgatgcgaaa gtggtggcgg aagccatcgc taagtacgac 2400

attaaagcag atcgtgcaaa tccgttatac gcataa 2436

<210>20

<211>1884

<212>DNA

<213> Actinobacillus succinogenes

<400>20

atgtcaaaac aaattcaaat tccagatatt ggtgcggatg aagttacggt tactgaagtg 60

atggtaaacg tgggtgatat cgttgaagtc gatcaatcga ttattaatgt agaaggcgat 120

aaggcatcaa tggaagttcc ttctccggaa accggcgtcg tgaaagaatt attagtgaaa 180

gtcggtgata aagtcaccac cggcactccg atgtttatat tggaagccgc aggttcagcc 240

tccgcagcac ctacacccca ggccgaagtc gctccggcag cggcacctgc agttgccgaa 300

gtgaaagatg tgcacgtacc ggatatcggc ggtgacgaag tcaacgtaac cgaaatcatg 360

gtaaaagtcg gcgacacggt gacggaagaa caatcattaa tcaccgttga aggcgataaa 420

gcgtcaatgg aagtaccggc gccgtttgcc ggcgtggtga aagaaatcag cgtgaaatcc 480

ggcgataaag tgtcaaccgg ttcattaatc atgaaattcg aagtagcggg ttccgcacct 540

gcggctacag ttgatcaacc tgcggtagcg gctccggcgg catctgcagc ggccgcggta 600

aaagatgtta acgtaccgga tatcggcggt gacgaagtca acgtaaccga aatcatggta 660

aaagtcggcg acacggtgac ggaagaacaa tcattaatca ccgttgaagg cgataaagcg 720

tcaatggaag taccggcacc gtttgccggc gtggtaaaag aaattatcgt gaaatccggc 780

gataaagtgt caaccggttc attaattatg cgctttgaag tagcgggagc agcgccagca 840

gccgcacctg caccggtatc tcaacctgcg gcagcggttc cggcggcaca atccgcacct 900

gccgccgccc ctgtatccgc gccggcagcc agtcaggatg cggtaacatc aagcgcaaca 960

tttgtccatg caacaccggt cgttcgtcgt ttagcgcgtg aattcggcgt gaatctggat 1020

aaagtaaaag gtaccggtcg taagggacgt atcttgaaag aagacgttca ggaatatgtg 1080

aaaaatgccc ttaaacaact ggaaagcggt gctgcgacag gtgcggcaaa tggcgccggt 1140

ttaggcttgt tgccatggcc gaaagtcgat ttcagcaaat tcggcgaagt agaagaaatc 1200

gaattgaccc gtatcaacaa gatctccggt gcaaatctgc atcgtaactg ggtgatgatt 1260

cctcatgtaa cccactttga tcgcgcggac attacggatt tagaagcgtt ccgtaaagaa 1320

caaaatgctt tagcggaaaa acaaaaactg ggtgtgaaaa ttacaccggt tgtgtttat 1380

atgaaagcgg tggcaaaagc cttagaagcg ttcccgcgtt tcaacagctc aattaccgaa 1440

gatgcgcaac gtctgattct gaaaaaatat atcaatgtcg gcgtagcggt ggatacaccg 1500

aacggtttag tggtacctgt atttaaagat gttaacaaaa aaggcattat cgaactttcc 1560

cgcgaattga tggaagtttc gaaaaaagcc cgtgagggta aattaaccgc atccgatatg 1620

caaggcggtt gtttcaccat ttccagtatc ggcggtttgg gtacgactca ctttgcaccg 1680

attgttaatg cgcctgaagt ggcaatttta ggggtatcta aatccgaaat ggctccggtg 1740

tggaacggta aagaatttat gccgcgctta atgcttccgt tgtcattatc tttcgaccac 1800

cgagtgatcg acggtgcgga cggtgcacgc tttaattactt ttattaacgg tgtgctttcc 1860

gacttacgtc gcttagccat gtaa 1884

<210>21

<211>1425

<212>DNA

<213> Actinobacillus succinogenes

<400>21

atgagcaaag aaattaaaac acaagttgtc gtgttgggtg caggtcctgc ggggtattcc 60

gccgcattcc gttgtgcgga cttaggattg gaaaccgtat tggtcgaacg ctattcaacg 120

cttggcggag tatgcttaaa cgtgggctgt attccatcca aagccttat gcacgtggca 180

aaagtaattg aagatgctaa acacgtcgaa caccacggcg tagtattcgg tgaaccgaca 240

atcgatttgg ataaaatccg agccggtaaa gacggcgtgg taggccgttt aaccggcggt 300

ttggccggta tggcaaaaat gcgtaaagtt accgtagtgg aaggcttggc aaaatttgca 360

gatagccaca ccctggtggc gacggatcgt gaaggtaatg cgactacaat taaatttgac 420

aatgcgatta ttgcggcggg ttctcgtccg attcaacttc cgtttattcc gcatgaggac 480

ccacgcgttt gggattctac ggatgccctc gcgttacgtg aagtaccgaa aaaattactc 540

gttatgggcg gtgggatcat cggtctggaa atgggaacgg tttacagtgc tttaggttcg 600

cagatagatg tggtggaaat gttcgatcaa gtaattcctg cggcggataa agacatcgtt 660

aagatcttta ctaaacgtat cgaaaataaa tttaacttat tgcttgaaac caaagtaact 720

gccgttgaag cgaaagaaga cggtattcat gtttccatgg aaggtaaagc ggcaaacgaa 780

acccgtgttt atgatgcagt attggtggct atcggccgta caccgaacgg taagctgata 840

gatgcggaaa aagccggagt agaagtggat gaacgcggct ttatccgtac cgataaacaa 900

atgcgcacga atgtgccgca catttatgct atcggcgaca ttgtcggaca accgatgtta 960

gcgcacaaag gcgtacatga aggtcatgtt gctgccgaag taattgcggg caaaaaacac 1020

tattttgatc ctaaagtaat cccttccatc gcttataccg aaccggaagt ggcttgggta 1080

ggtaaaaccg agaaagagtg caaagctgaa aatctgaatt atgaagtggc gacatttcca 1140

tgggcggcat ccggtcgtgc tatcgcctcc gattgtgctg acggtatgac gaaattgatt 1200

ttcgacaaag aatcgcaccg tattttaggt ggtgcgattg tcggtactaa tgccggcgaa 1260

ttactgggtg aaatcggttt agccatcgaa atgggttgcg atgcggagga tattgcctta 1320

actatccacg cccacccgac tttacacgaa tcggtcggtt tggcggcgga agtatttgaa 1380

ggctccgtta cggatttacc aaatgcgaaa gccaagaaaa aataa 1425

<210>22

<211>2023

<212>DNA

<213> Actinobacillus succinogenes

<400>22

aaataccggc tttcgcgcaa gcaaacggca atttagctac agatgttaat gatgccaccg 60

cacctttttg gtcgcgaccg tgcattgggt ttgcacccgg tccgaatggt gcgcctgcgc 120

gacgaccgtc cggagtatta ccggttttct taccgtatac cacgttagaa gtgatagtca 180

ggatagattg tgtcggtacg gcattacggt aagttttgtg agtttgaact tttttcatga 240

aacgttcaac caaatcaacc gctaaatcat cgacgcgcgg atcgttgtta ccgaattgcg 300

gatattcgcc ttcgatttcg aagtcaatcg ctacatttga cgctacgacg ttaccgtctt 360

tgtctttaat atcgccgcga atcggtttaa ctttcgcgta tttgattgca gacaatgagt 420

cagctgcaac agacagtccg gcaataccgc aagccatggt acggaatacg tcacgatcat 480

ggaaagccat caatgccgct tcataagcgt atttatcgtg catgaagtgg atgatgttta 540

atgcggttac gtattgagtc gccaaccaat ccatgaagct gtccatacgt tcgataacag 600

tatcgaagtt taatacttca tcagtgatcg gtgctgtttt agggccgacc tgcataccgt 660

ttttctcgtc gataccgccg ttgatagcgt ataacatagt tttcgctaag ttcgcacgag 720

caccgaagaa ctgcatttgt ttaccgacaa ccatcggaga tacgcaacat gcgattgcgt 780

aatcatcgtt gttgaagtca ggacgcatta aatcgtcgtt ttcgtattgt acggatgaag 840

tatcgatgga aactttcgca cagaaacgtt taaacgcttc aggtaattgt tcggaccaaa 900

gaatcgttaa gttcggttcc ggagacgtgc ccatagtgta taaagtatgt aatacacgga 960

aactgttttt agtaaccaac ggacgaccgt ctaatcccat ccctgcgatg gtttcggttg 1020

cccacatcgg gtcgccggag aataattgat cgtattcagg cgtacgtaag aaacgaacca 1080

tacgtaattt cataattaag tggtccatta attcttgcgc ttgttgttct gtgattaaac 1140

cgcgttttaa gtcacgttcg atatagatat ctaaaaatgt tgaagtacga ccgaaagaca 1200

ttgccgcacc gttttgtgat ttaaccgcag ctaaataagc gaagtaagtc cattgaatag 1260

cttcctgtgc attcattgca ggaccggaaa tgtcataacc gtaagacgcc gccatttctt 1320

tgattttacc taacgcacga tgttgttcgg caatttcttc acgtaactga atagtagcct 1380

gaatgtcttc acccgcttct aaacgcggtt gcaatgaatc gaattgagct tttttatctt 1440

tcattaagta atcgataccg tataccgcta aacgacggta gtcaccgata atacgaccac 1500

gaccgtaagc atccggtaaa ccggtcagca cacctgattt acggcaacgt aaaatgtccg 1560

gcgtataaac gtcgaataca ccttggttat gggttttacg gtattcggtg aaaatgaatt 1620

cgactttagg atctaaagta cggccgtaaa cctgacaaga acctttgatc attttaatcc 1680

cgccgaacgg catgattgca cgttttaacg gtgcgtcagt ttgcaaaccg acgatttttt 1740

ccaaatcttt gtcgatatag cccggtttgt gggaagtgat ggttgacgga gtatgttcat 1800

caaaatccaa cggcgcatgg gttttgtttt cgattttgat gccttccatc acgctgttcc 1860

acaattcagt ggtcgccgga gtcgcatcag ccaagaatga ttcgtcgcct tcgtacggtg 1920

tatagttttt ttggataaag tcacgtaaat ttacgccttc ctgccattca ccgggaacga 1980

atccttccca tgctttttgt tgagcttcag ttaattgagc cat 2023

<210>23

<211>2625

<212>DNA

<213> Actinobacillus succinogenes

<400>23

atgaataaca ctgttgaaaa tgcagtcggt ccggcacaga cggaagtcaa ttcgctggta 60

gagaaaggtt tggtcgcact ggaaaaattc aggggactca atcaggaaca ggtggattac 120

atcgtcgcta aagcatcggt tgcggcgctg gaccaacacg gcacattagc attacacgca 180

ttagaggaaa caggacgcgg cgtgtttgaa gacaaagcga ctaaaaacct gtttgcctgc 240

gaacacgtgg taaataaaat gcgtcactgg aaaaccgccg gtataatcag tgacgatgac 300

gttaccggta tcaccgaaat cgccgatccg gttggcgtga tatgcggtat tacaccgact 360

actaatccca cttctaccac tattttcaaa tccttgatcg cactaaaaac ccgtaatccg 420

attgtattcg ccttccatcc atcggctcaa caatcctccg ctcacgccgc ccgtatcgtg 480

cgtgatgccg ctttagccgc aggcgcaccg gaagattgca ttcagtggat tgaacatccg 540

tccatggaag gaaccaatgc actgatgaac cactcgggta tcgctaccat tctggctacc 600

ggcggcaacg ccatggtaca agcggcgtac tcctgcggta aaccggcgct cggcgtcggt 660

gcgggtaacg taccggctta tgtagaaaaa tccgccgata ttaaacaggc ctgccacgat 720

atcgtaatgt cgaaatcctt tgataacggc atgatatgcg catcggaaca agctgccatc 780

attgatgcgg aaatttacga cgaatttgta aaagaactga aatcctacgg tgtctatttt 840

gtcaataaaa aggaaaaagc gttgctggaa gcctttatgt tcggtgtaaa aggcaacagc 900

gctaactgcg ccggcgccaa attaaacgcc gacgtggtgg gaaaatccgc ttactggatt 960

gcacaacagg ccggctttga agtgccgccg aaaaccaata ttctggcggc ggaatgtaaa 1020

gaagtcagtc cgaaagaacc gttaaccagg gagaaactgt ctccggtgct cgccattctg 1080

aaatcccgct cgacggagga ggggctgctg atggcggaag ccatggtgga gtttcacggt 1140

ttgggtcact ccgccgccat tcacactaaa gacgccgaat tggccaaacg tttcggtgag 1200

cgggtaaaag ccattcgcgt tatctggaat tcaccctcca ctttcggcgg tatcggtgac 1260

gtatacaacg cctttctgcc ttccttaacg ctgggatgcg gttcgtacgg taaaaattcc 1320

gtaagtaaca acgtcagcgc catgaattta gtcaatatca aacgcgtagg cagacggaga 1380

aataatatgc aatggtttaa agtgccttca aaaatctatt ttgaacgtga ttcaattcaa 1440

tattacaat ccgtaccgga catgaaacgc gtagtgatca taaccgaccg tactatggtg 1500

gacttagggt tcgtgcaaaa aatcgcacac cagctggaat cccgccgtga tccggtttct 1560

tatcagcttt ttgccgatgt agaaccggat cccagcatcc agaccgtacg ccgcggagta 1620

gatttaatcc gcaacttcaa accggatagt attatcgccc tgggcggcgg ctcggcgatg 1680

gacgcggcaa aagtaatgtg gctgttctac gaacaaccgg aaatagattt tcgcgatttg 1740

gtgcaaaaat ttatggatat acgtaaacgt gccttcaaat tcccgtcatt gggtaaaaaa 1800

gcccgctaca tcggtattcc gaccacatcc ggtacgggtt cggaagttac gccgtttgcc 1860

gtaattaccg aaggaaataa aaaatatccg attgcggatt actcgctcac tcctaccatc 1920

gcgctggtgg atccggcact ggtgatgact gttcctgcca atgtcgcggc ggacaccggt 1980

ctggacgtat tgactcacgc gacggaagcc tacgtatccg tgcttgccaa cgattatacc 2040

gacgggttgg cgttgcaggc gatcaaactg gtattccgct atctggaaaa atccgtcaaa 2100

gagaaagatg cggaagccag ggaaaaaatg cacaacgcct caaccattgc cggtatggcc 2160

ttcgccaacg catttttagg catgaatcac tccctcgctc acaaactcgg cggttatttc 2220

cacacgcccc acggacgtac taatgcgatt ttaatgccgc atgtcattcg ttataacggc 2280

accaagccga ctaaaaccgc tacgtggccg aaatacaatt attacaaagc ggacgaaaaa 2340

tatcaggata tcgcccgttt gctgggtcta ccggcagcca cgccggcgga aggagtaaaa 2400

tcctacgcga aagcggttta cgatttagcc gtgcgttgcg gtattaaaat gtcttttaaa 2460

gagcagggac tggacgagca agcctggatg gatgcccgcc atgaaatcgc gctgcttgct 2520

tatgaagatc agtgttcgcc ggcgaacccg cgtttaccga ttgtcgcgga tatggaagac 2580

attctggcca acgcttacta cggttacgac gaaaatcaat attaa 2625

<210>24

<211>1119

<212>DNA

<213> Actinobacillus succinogenes

<400>24

atggaattta tcaaaacccg agcggcggtg gcatgggcgc cgaatgagcc gttgaaaatt 60

gaagaaatcg atttaatgcc gccacaggct ggcgaagtat tggtgcgaat tgtggcgacg 120

ggcgtgtgtc ataccgacgc ttacacactt tccggacagg attcggaggg tgtgtttccg 180

tgtattttgg ggcacgaagg cgccggcatt gtagaagcgg tgggggaagg cgtgaccgat 240

tttaaagtcg gcgaccacgt gattccgctt tatacggcgg agtgcggcaa gtgtaaattc 300

tgcttgtcgg gcaaaaccaa tctttgttcg gcggtgcgtg aaacccaggg taagggctta 360

atgcctgacg gtacggtacg ttttttcaag gacggtcaac cgattattca ctatatgggg 420

acttcgactt tctcggaata caccgttgtg tcacagtatt cattggcaaa aattcaagaa 480

aatgcaccgc ttgatgaagt ctgcttgctc ggctgtggcg taactacagg tattggcgca 540

gtcacaaaaa ccgccaaagt gaaaaaaggc gacaccgtgg caatcttcgg cctgggcggt 600

atcggtttgg cggcgattat cggcgcacga atggcggaag cgggccgtat tatcggtatc 660

gacaccaacc cggctaaatt tgagaaagcc aaagaactgg gtgcaaccga ctgcattaac 720

ccgaaagatt tcgataaacc gattcacgaa gtcatttatcg aaatgaccga cggcggcgtg 780

gatttctcct ttgaatgtat cggcaacgtg gacgttatgc gttcggcact ggaatgctgc 840

cacaaaggct ggggcgaaag tgtgattatc ggcgtagcgc cagcaggtgc cgaaatccgc 900

acccgcccgt tccagttggt cactggtcgc gtatggcgtg gttcggcgtt tggcggcgtg 960

aaaggtcgta ccgaattacc gggtattatc gacaaattta tgcaaggtga attcaaactg 1020

cgtgatttca tcacccatac catgccgttg gaagacatca ataaagcttt cgacttaatg 1080

cacgaaggca aatcaattcg cacggtgatc cactactaa 1119

<210>25

<211>273

<212>DNA

<213> Actinobacillus succinogenes

<400>25

atgatacaac gacaatttac cgtttacgga tgtgtgcagg gcgtgggttt tcgttttttt 60

accgcccgcg aagccaataa attaggcatt cagggttatg tgaaaaatca agccgacggt 120

tcggtgcggg ttgttgccgc aggttccgac cggcaagtcg ccgcattcag agactggctg 180

gagcaggggc cgcctacggc ggaagtgacg aatttacttg aacaggaata cacgggcgga 240

cgggtattca gcgattttac catcgaacgt tag 273

<210>26

<211>231

<212>DNA

<213> Actinobacillus succinogenes

<400>26

atgacgaaag atcttaatgt atttgataaa cattacggtt tattgattaa cggtgagtgg 60

accgatggtt ccgaaggtaa aactttaacg gcgcataacc ctgcaaacgg gacggaactg 120

gcgactttta ttgacgcaac ggatgccgat gtcgatgcgg cggtaactgc cgcgcaggag 180

gcttttaatc ctggcgccat accagtgcgg cggagcgggc ggcaattttg a 231

<210>27

<211>1257

<212>DNA

<213> Actinobacillus succinogenes

<400>27

ttgaataaaa tcgccgatgt gattgatgac aatacggaat tattcgcctt gcaggaaacc 60

ctggataacg gcaaaccgat tcgtgaaaca cgggcggcgg atattccgtt ggcagcggat 120

catttccgtt attttgcggc ggtgattcgt tccgaggaag gcagcgccaa tcagttggac 180

gacgaagat ttgtctctgat tctgcgcgaa ccgattggcg tggtcggaca aattattcct 240

tggaatttcc cattcctgat ggcggcatgg aaaattgcgc cggcattggc ggcaggttgt 300

acggtggtga ttcacccgtc ttcttctacg tctttgagcc tgctttcttt ggcgcagaaa 360

attaatcact tattaccgaa aggcgtgttt aatgtgatta ccggcaaagg ctctaaatcc 420

ggcgaatata tgctacacca caccggcttt aataaactgg cgttcaccgg ttcaaccgaa 480

atcggtcgta aaatcggggt ggcggcggca gaaatgttga ttccgtctac gctggaattg 540

ggcggtaaat cggccaatat tttctttgat gatatgccgt ttgataaagc attggaaggc 600

gcgcaaaaag gtattttgtt taaccaaggt caggtttgct gtgccggttc gcgaattttc 660

gtacaagaaa atatttacga taaatttatt gcggcgttga aagaggaatt caaaaaagtc 720

aaagtcggct tgccttggga agacgatacc caaatggggg ctcaggtcaa ttccaaccaa 780

attaaggtaa tcagtaaata tgtggatatc gccaaagaag aaggttgcga aattattatc 840

ggcggcgaaa aagcgacgga tccggtgttg gcgaaaggtg agtttttcca accccacctta 900

attctggcgc cggataacac taaacgggtg gcgcaggaag aaattttcgg cccggtcgcc 960

gtggtaatta agttcaaaga cgaagcggat gtgattcaca tggcgaatga tagcgaatac 1020

ggtttaggcg gtgccgtatg gacgcataat attaatcggg cgttacgtgt ggcgcgggcg 1080

ttagaaaccg gtcgtgtatg ggtgaactgt tacaaccgat tgccggcggg cgcgccgttc 1140

ggcggttata agacttccgg tatcgggcgt gaaacccata aaatgatgct ggcggcctat 1200

acgcaggtga aaaatatttt catcagcacc cgtgaggagc gcgagggaat gtattaa 1257

<210>28

<211>1070

<212>DNA

<213> Actinobacillus succinogenes

<400>28

atgtccaaat tagttttaat tcttaactgc ggtagctcat ctttaaaatt tgctatctta 60

gatcccgtat ctggtgaaga aaaattatca ggtttagccg aagctttcta tcttccggaa 120

gcgcgtatca aatggaaatt gcacggtgaa aaaggcaatg cggatttagg tgccggagct 180

gctcacagtg aagcgttaaa ctttattgtt aaaaatattt tccctttaga cccttctttg 240

caggaaagta tcgtagcaat cggtcaccgt atcgttcacg gcggtgagaa atttacctct 300

tccgttatcg tcaccgacga agtagttaaa ggtatcgaag acgctattca attcgcaccg 360

ttgcacaacc ctgcccactt aatcggtata aaagaagcgt tcaaaatctt cccgcatctg 420

aaagataaaa acgtagtcgt tttcgacacc gcattccatc aaaccatgcc tgaagaagca 480

ttcttatacg ctcttcctta ttctttatat aaagaacacg gtattcgtcg ttacggcgca 540

cacggtacca gccactattt tatcagccgt gaagcggcaa aacgtttagg tgtcgcggaa 600

gataaaatta acgtgattac ctgccactta ggtaacggcg gttccgtttc cgcaatccgc 660

cacggtgaat gtatcgatac ctcaatgggc ttaactccgt tagaaggtat cgtaatgggt 720

acccgttgcg gcgacatcga tccggcgatc atgttctata tgcatgatac tttgggcatg 780

tccgtagaag aaatcaacac gaccttaacc aaaaaatccg gtatcttagg tttaacggaa 840

gtgaccagtg actgtcgctt tgcggaagac aactatgaaa gtgatgacga atcgttacgt 900

gtaccggctc gtcgcgcaat ggacgtttac tgctaccgtt tagcgaaata catcggttct 960

tatatggcgg ttatcggtga gcgtttagac gccatcgtgt ttaccggcgg tatcggtgaa 1020

aactccgctc acgtacgtga aatcacgttg aatcacttta aaactgttcg 1070

<210>29

<211>270

<212>DNA

<213> Actinobacillus succinogenes

<400>29

atgactccag cagaactctt tggtgaaggg attaacctga tgctttcagg tatgggtttc 60

gttattacgt ttttgctcat tttaatttgg gcaatcacta cgatgtcaaa actcattaac 120

cgctttttcc ccgaacccgt taaacaatct aaaccttcgc aaaaaccgac cgcactttcc 180

gctgcggtac aaggaaatga tttagatcgt ttacgtcccg ttatcgtggc ggctatcgcc 240

catcatcgcc gttctcaagg cttaaattaa 270

<210>30

<211>1809

<212>DNA

<213> Actinobacillus succinogenes

<400>30

atggctaaaa aaattaaagt taccgaatta gtacttcgtg atgctcatca atctcttttc 60

gcaacacgtt tacgccttga cgatatgttg cctatcgcag cggaattaga cgatatcggc 120

tactggtcat tggaagcctg gggcggtgcg aattcgata gctgtattcg ttttttaggc 180

gaggaccctt gggtacgttt gcgcgagctg aaaaaagcct gcccgaaaac tccgttgcaa 240

atgttattgc gcggacaaaa cttattaggc taccgtcatt atgccgatga cgtggtggag 300

cgttttgttg aacgttgcgt ggcaaacggt atggatgttt tccgtgtatt tgatgcgcta 360

aacgatccgc gaaatatgaa agccgcatta caagcagtga aaaaacaggg cggtcacgca 420

caaggtacat taagttatac caccagcccg gttcacacat tacaaacttg gttagacact 480

actgaacaat tattggaaat cggcgtggac agcctggtta ttaaagatat gtcaggcatt 540

ttaaacccaa tggctgcggc cgaacttgta ggcgaaatca aaaaacgttt tgatatcgaa 600

ttacatttac actgccacgc tacgaccggt atgtccgaaa tggcgttgct gaaagctatt 660

gaagcaggtg tcgacggggt agatacggcg atttcttcaa tgtccggtac ttacggtcat 720

ccggcaacgg aagcgattgt ggcaaccttg caaggcacag cattcgatac cggattagac 780

attcctcgtt tggagaaaat tgccgcctat ttccgtgacg tacgtaaaaa atacgcaaaa 840

ttcgaaggtc agttacgtgg tgtggacagc cgtattttag tggcgcaagt gccgggcggt 900

atgttaacca atcttgaaaa tcaactcaaa caacaaaatg cgtcggataa actggacaaa 960

gtattagaag aaatcccact cgtgcgtaaa gatttgggtt atattccgtt ggttacaccg 1020

acttcgcaaa ttgtaggtac acaagctgtt attaacgtgt taatgggtga acgttacaaa 1080

accatcgcca aagaaaccgc cggcgtattg aaaggcgaat acggtaaaac tccggcaccg 1140

gttaatgccg aattacaagc gcgtgtatta gaaggtgggg aaccgatgac cgaccgtcct 1200

gcagatcacc ttgaaccgga aatggctaac atcgaagcgg acgttattgc acaagcaaaa 1260

gaaaaaggtg tggctttatc agaaaattcc gtagatgatg cgttaattgt ggctttatc 1320

ccgcaaattg cttggaaatt cttggaaaac cgtaataatc cggcagcctt tgaaccggca 1380

cctaccgcag aaagtgccgc ggaaaaaccg caggaaaaag cggcaccgaa agcccaatcc 1440

ggttcggccg tctataccgt tgaagtggaa ggtaaagcct tcgtagtgaa agtcagcgaa 1500

ggcggtgata tttcggctat cgcaccggca accgctcctg ctgcggctgc gcaagctgct 1560

ccggccgcag caccggctcc cgcaccttca gcgggcggcg tgcaggttac cgcaccgatg 1620

gcgggcaaca tctggaaagt gattgcaacc gaaggtcaaa ctgttgcgga aggagacgtt 1680

ctgtttatc tcgaagcaat gaagatggaa accgaagtta aagcggcgca agccggtacg 1740

gttcgcaata tccaagtgaa agcgggtgac agcgtatccg tcggcgacgt tgtgatgacc 1800

ttggcataa 1809

<210>31

<211>1308

<212>DNA

<213> Actinobacillus succinogenes

<400>31

atggatagta ttattgcatt aatccaaggt acgggaataa tgcacatgga atgggggcaa 60

gccgtaatga ttgccatcag tgtgctgtta ttatggttgg ccatatcgaa gaaattcgaa 120

ccgttgttgt tgttacctat cggtttcggc ggattgttat ccaatattcc ggaagccggt 180

ttggctatga ccgcattgga aaatctgttg catttgggtt cgcccgaaca aatcggtatg 240

attgccgcaa aactgggggt ggcggctgat cccgccgcca tcaaatcggc actttcttcg 300

gtaccggctt caacggttaa ccagcttgaa accatgtcaa tggatatggg ttattccgcc 360

gggatattgg ctttattcta caaagtcgct atcagttacg gcgtggctcc gctcattatt 420

tttatggggg ttggggcgat gaccgacttc ggcccgttat tagctaatcc aaaaaccctg 480

ttgttaggtg cggcagcgca attcggtatt tttgctactg tattgggtgc tttaacgctg 540

aactatttcg gtattatcga gtttactcta ccgcaagcgg catctatcgg gattatcggt 600

ggtgcggacg gtccgaccgc tattacctg accagtaaac tggcgccgga attgctgggt 660

gcaatcgcag tggcggctta ttcctatatg gcgttggttc cgcttattca gccgccgatt 720

atgaaagctt taacgacgga agaagaacgt aaaatccgca tgactcaatt acgtagcgtt 780

agtaaccggg agaaaattat tttcccgatc atcttattat tactcgtcgc tttaattactt 840

ccggatgcgg caccgttgtt ggggatgttc tgtttcggta acttaatgaa agtgagcggt 900

gtggttgatc gcttaagcga tacgacccaa aatgcgttaa ttaatatcgt tacgataatt 960

ttaggattat cggtcggttc aaaactgatt gccgacaaat tcctgcaacc gcaaactctg 1020

ggtattttgt tattaggcgt ggtggctttc tgtatcggta cgggatgcgg cgtgttaatg 1080

gctaaattga tgaataaatt aagtaagacc aaagtcaatc cgcttattgg tgctgccggc 1140

gtatcagccg taccaatggc tgcccgggtc gccaataaag tcgggttaga agcggacagc 1200

caaaactttc tacttatgca tgccatgggg ccgaatgttg ccggtgttat cggttccgct 1260

attgccgcgg gtattatgtt gaaatatgtt tcggcattta ttatgtaa 1308

<210>32

<211>1695

<212>DNA

<213> Actinobacillus succinogenes

<400>32

atgaaaccgg ttttaaattt actccaactc aaaaaggaaa atactatgca aaatattaac 60

ccgaccaaaa caacagcatg gaaagcatta gaacagcata aagcggaatt agccgaaact 120

acgatcaaag atctttttca acgggaacag aatcgtttcg ataattattc attaactttt 180

aataacgaaa tgttagtgga tttcgctaaa aataaaatta ctcaggatac tttaaaatta 240

ttgcgtcaat tggcaaaaga atgtgcattg gacgaagcca aagaagcgat gtttacgggc 300

gaaaaaatca accgtacgga aaatcgcgcc gtattacata ccgccttgcg taaccgctcg 360

aacaagcctg tgttggtcga cagtaaagat gtgatgccgg aagttaatga agtcttggca 420

aaaatgaaag ctttcagcga acgcgtaatt tccggcgaat ggaaaggcta caccggcaaa 480

gccattaccg atgtggtgaa tatcggtatc ggcggatcgg atctcggccc ttacatggta 540

acggaagctt tacgtccgta taaaaatcac ttgactatgc attttgtttc caatgtggac 600

ggtactcata ttgcggaagt ctttaagaaa accgacccgg aaaccacttt atttttagtg 660

gcgtcaaaaa cctttatactac tcaggaaacc atgaccaatg cgaaatctgc ccgcgattgg 720

ttcctggcgg cggcaaaaga cgaaaaacac gtggcaaaac attttgccgc actttcgacc 780

aatgcgacgg aggtggcgaa attcggtatt gataccgaca acatgttcgg tttctgggat 840

tgggtcggcg gacgttattc attatggtcg gctatcggtt tgtccattgc gctttccgta 900

ggttttgaca atttcgaaca attattggcc ggcgctcatg aaatggataa aatttccgg 960

aatacgccga ttgaacaaaa tattccgacc attggcgt tagtaggggtt atggaatacc 1020

aatttccaag gtgcgcaaac cgaagcgatt ttaccttacg atcaatatct gcaccgtttt 1080

gcggcatatt tccaacaggg taacatggaa tccaacggta aatacgtggg acgtgacggc 1140

gaagtaatta acaattatca aaccggtccg attatttggg gcgaaccggg taccaacggt 1200

caacacgcgt tctatcaatt aattcatcag ggtaccacgt taattccttg cgatttcatt 1260

gcaccggcga aaactcataa tccgttggcg gaccaccaca gcaaattact gtctaacttc 1320

tttgcccaaa ctgaagcgct ggctttcggt aaaaccaaag aaaccgtgga agaagaattt 1380

ttgaaagccg gcaaaaattt agacgaagtg aaaaatgtgg tgccgttcaa agttttcgcc 1440

ggcaacaaac cgaccaactc gattttatg caagaaatta cgccgtttag tttaggcgct 1500

ctaattgcca tgtacgaaca taaaatcttc gtacaaggta ttatcttcaa tattttcagt 1560

ttcgaccaat ggggcgtaga attaggtaaa caactggcaa accgcattct gcccgaatta 1620

agcggcaatg agccaatcaa tagtcacgat agctcgacca acggcttaat caaccaattc 1680

aaagcttggc ggtaa 1695

<210>33

<211>966

<212>DNA

<213> Actinobacillus succinogenes

<400>33

atgatcaaaa aaattgcagt cttaacaagt ggtggtgacg caccgggaat gaatgccgca 60

attcgtgccg tggtacgtgc gggattatcc gaaggattag aagtatacgg tgttttcgac 120

ggttaccaag gactttttga gaaccgtatt caaaaacttt cgcgctatag cgtgtccgat 180

gtcattaacc gcggcggaac gtttttaggt tccgcccgat tccctgaatt caaacaaccg 240

gaagtgcggt cgaaatgcgc cgaaatttta cactcgcacg gcattgacgc tttagtggtt 300

atcggcggtg acggttctta taccggtgca aaattattaa cggaagaaca cggtttccct 360

tgtatcggct tgccgggtac gatagataac gatattccgg gcaccgacta caccatcggt 420

tatcaaaccg cattggaaac cgccgttgac gccatcgacc gcttgcgcga taccagcggt 480

tctcaccatc gtatttccat tgtggaaatc atggggcgac actgcagcga tttaaccatt 540

tgtgcggcaa tcgccggcgg ctgcgaatat atcgtcgcac cggaagtcgg attcaatcag 600

gaagaattaa tccaacaaat cgaactcagt cttgccaacg gtaaacgtca tgcgattatc 660

gccattaccg aattagtcac ggacgtacac aaattagcca aagatattga agctcgggtc 720

ggcaacgaaa cccgtgcaac cgtgttaggc cacgtacaac gcggcgggtc cccttgcgct 780

tttgaccgta ttttagcctc ccgcatgggc gtgtacgcag tagaattatt gctgcaaggt 840

cacaaaggac gctgcgtggg tatccagcag gaaaaactgg tacatcacga catcatcgaa 900

gccattgaca gtatgcgccg tccgtttaaa tccgattacc tggaagtatc caaaaaatta 960

ttctaa 966

<210>34

<211>1074

<212>DNA

<213> Actinobacillus succinogenes

<400>34

atgacagcaa agaaaattta tttcggtacc aatctgaaaa tgtataaggg gaatgccgag 60

gtcgtgcaat atctctccga attatctgat tttgccacta cctttaaatc ggaatatgac 120

attcaattgt ttgtaattcc gtcttatacc acattaaaag atgccgtgga attagtgaaa 180

tcaaagacgg gtcggcctaa aattaaaatt ggcgccccaga atatgaatcc gaatgacaac 240

gggcagttta ccggtgagat ttcaccgtta atgcttaaag aattgggaat cgaactggtg 300

atgatcgggc attctgaacg tcgtcatgtg atgaaggaaa ccgatcagga agaaaatgag 360

aaagtgcttg cgtctttgaa gcataatttc attacgctat tatgtatcgg tgaaacgctg 420

gagcaaaaaa attacaacat ctccgatgaa gtattgcgaa ctcagctaaa aatcggatta 480

cagggtgtta ccgccgaaca attatctaag ctgtggattg cttatgagcc ggtgtgggcg 540

attggtacgg gaggaattcc ggcaagcgcc gaatacgccg atgaaaaaca tgccgttatt 600

aaacaatgtc tgtttgaact ttttgctgaa gaaagtaaaa aaataccagt gctatatggc 660

ggtagtgtta atcctgaaaa tgctaatagc ttaattacca aaccgtatat tgatggtcta 720

tttatcggcc gtagtgcatg gaatacgtca aattttcatg cattaattgc tgatgtattg 780

aatacgcttt ctggcagtaa aattgacccg ataatcaata aatttacgga aacagcgata 840

caactcgttg ataaattggg ggggaaggat aatattagtg ctttaaccca ctgtgctact 900

cgtattcgcg tagtgttaag aaatgatgga aaaatggata aatcagctat tgaaaaaatt 960

gattgtgtta aagggttacc ttctattacc aatcaatatc aaattatttt agggtgcagga 1020

atagtaaatc aggttcatga ggaaatggta aaattactgg ctagatcatt ataa 1074

<210>35

<211>786

<212>DNA

<213> Actinobacillus succinogenes

<400>35

atgaccaaga aaatctattt cgggactaat ctgaaaatgt acaaaggtaa tgcggaagcg 60

gtggattatc taaccaaact gtccgaatta gcggatacat tgccggcgga taaaaatctg 120

gaattatttg tcatcccttc atttactacc ttaagcgacg ctatcgccgc cgtacgcagc 180

ataccttcca aacatccgat tatgatcggt gcgcaaaata tgaatccgaa cgattcgggg 240

caatttaccg gcgatatttc gcccctcatg ctgaaagaac tgggcgtaca attagtaatg 300

atcggacatt ccgaacgacg tcacgtattc cgtgaaacgg atcaggaaga aaatgctaaa 360

gtattggcgg ctcttaaaca tcaatttgtc actttacttt gtgtcggtga aaccctggaa 420

caaaaaaact acggtatttc cgacgaaata ttaagaaccc aactaaaaat cggtctacac 480

ggcgtcacac cggatcaatt atcgttgctg cgtgtcgctt atgagccggt ttgggcaatc 540

ggcaccgccg gtatccccgc ttccgccggt tatgccgatg aaaaacaagc ggtaatcaaa 600

caatgtctga tcgaattatt cggtgaagaa ggcgacaaga tcccggtttt ctacggtggc 660

agcgtgaatc cggaaaatgc accggaatta ttcgtccaac cgcatatcga cggattattc 720

atcggacgca gcgcctggga agtggataaa ttccatgcgc taatgtccgg tttattgaca 780

aaataa 786

<210>36

<211>771

<212>DNA

<213> Actinobacillus succinogenes

<400>36

atggcatctc gtaaaccatt agtaatgggt aactggaaat taaacggcag taaagcgttc 60

accagagaat taatcgaagg cttaaaaacc gaactggccg gcgtagaagg ctgtgacgtc 120

gctatcgcac cgccggtaat gtacttggct gaagcggaaa ccgcgcttgc cgacagcaaa 180

atagccttag gttcacaaaa tgtggacgta aacgtaaaag gcgcgttcac cggtgacatc 240

tctaccgaaa tgttaaaaga tttcggtgcg aaatatatca ttatcggtca ttccgagcgc 300

cgtacttatc acaaagaaag tgacgaattc atcgcgaaaa aattctccgc cttaaaagaa 360

gcgggtttaa ccccggtttt atgtatcggt gaaaccgaag aggaaaatga agcgggcaaa 420

acggaagaag tggtttcccg tcaaattgat gccgtcatca acgctttagg cgtagaagcc 480

tttaacggtg cggttatcgc ttatgaaccg atttgggcaa tcggtaccgg caaatccgcc 540

actccggctc aggcacaagc ggtgcatgct tttattcgcg ctcatattgc ggcgaaatct 600

caagcggtag cggatcaagt catcatccaa tacggcggtt ccgtaaacga tgcaaacgca 660

gccgaactgt tcacacaacc ggatattgac ggtgcattag tgggcggcgc gtcattaaaa 720

gctcccgcgt tcgcagtaat cgtaaaagcc gcggcggcag cgaaggctta a 771

<210>37

<211>1014

<212>DNA

<213> Actinobacillus succinogenes

<400>37

atgaatcgag ctttagccat tgaattttca cgtattaccg aagccgcagc gcttgccgct 60

tttgcctggt tgggacgcgg tgataaaaat gcggctgatg acgccgccgt caaagctatg 120

cgttttatgc tgaaccaaat tcacatggat ggggaaatcg ttatcggtga aggcgaaatc 180

gatcaggctc cgatgctcta tatcggtgaa aaattgggtt ccggcatggg ggaagtggta 240

tcaattgcag tggatcctat cgacggtacg cgaatgacgg caatggggca ggcaaatgcc 300

attgccgtgt tggcggcggg cggtaaaaat aattcctgc gtgcaccgga tatgtatatg 360

gaaaaattgg tggtcggtcc tgaattaaac ggcatgatcg atttgaatct gcctatcgaa 420

cagaactgtc ggcgcgtcgc acagcgtttg gggaaatccg tcacacaatt aaccgtcgcc 480

gtgcttgcca aaccccgcca tgaagaagtg attackcgta ttcagaatct gggcgcccgc 540

gtaatgtgta ttccggacgg cgatgtggcg gcgtcattgt tatgctgttt accggacggt 600

ggtatcgata tactttacgg catcggcggc gcgccggaag gcgtgatatc cgccgccgcg 660

gttcgcgctt tgcacggcga tatgcaagca cgtttactgc cgcgtaatca agtgaaagag 720

gatacgcctg aaaatcgaaa ggttggcgaa caagaaatcc gtcgctgtca tgaaaacggt 780

gtggaagtga atacggtgtt gaaattggac gatctcgcca aagacgataa tcttgtgttt 840

tcggcaacgg gtattaccaa cggcgatttg ctcaagggca ttcaacgcaa aggaaattta 900

gccaccaccg aaaccttact gattcgcggt aaatcccgca cgattcggcg tattcaatcc 960

atgcatttatc ttgatcgtaa agatgcggaa atttataagt taatcggtga atag 1014

<210>38

<211>376

<212>DNA

<213> Actinobacillus succinogenes

<400>38

atggctaaat tattagacat cgtaaaaccc ggcgtattaa ctggcgatga cgtttcaaaa 60

gtatttgcat acgcaaaaga aaacggcttc gcaattcctg cagtaaactg tgtgggctca 120

gactctgtca acgctgtttt agaaactgca gcacgtgtaa aagcgcctgt aattattcaa 180

ttctctaacg gcggcgcttc attctacgca ggtaaaggcg tgaacctgca agcggcattc 240

gtccggatgt gttaggtgct atcgccggtg caaaacacgt acatacttta gccgctgaat 300

acggcgtgcc ggtgattctt cacaccgatc actgcgcaaa aaaattactg ccatgggtga 360

cggcttatta gacgca 376

<210>39

<211>966

<212>DNA

<213> Actinobacillus succinogenes

<400>39

ccgtgccgca aacaacgcga tgatatcgaa gttgtaggta tcaacgactt aatcgacgtt 60

gaatacatgg cgtacatgtt aaaatacgat tcaactcacg gtcgtttcga tggttcggtt 120

gaagtgaaag acggtaactt agtagttaac ggcaaaacaa tccgtgtaac ggctgagcgt 180

gatccggcaa acttaaactg ggcggcaatc ggtgttgata tcgcagtgga agcaaccggt 240

ttattcttaa ctgacgaaac cgcacgtaaa catattaccg ccggtgcgaa aaaagtagtt 300

atgaccggtc cttctaaaga cgcaactcca atgtttgtac gcggcgttaa ctttaatgct 360

tatgccggtc aagatatcgt atctaacgca tcttgcacaa ctaactgctt ggcaccgtta 420

gcacgcgtta tccatgaaac cttcggtatc aaagacggtt taatgacaac ggttcacgca 480

acgacggcaa ctcaaaaaac agttgacggt ccttcaatga aagactggcg cggtggtcgc 540

ggtgcggctc aaaacatcat tccttcatca accggtgccg ctaaagccgt aggtaaagta 600

ttacctgcat taaacggtaa attaaccggt atggcattcc gtgttcctac tccaaacgtt 660

tctgtcgttg acttaaccgt taacttggaa aaagccgctt catacgaaac tatcaaagcg 720

gcaatcaagg atgcggctga aggtaaaact ttcaacggcg aattaaagg cgttttaggt 780

tacactgaag atgctgtagt ttctacggac tttaacggtt gcgcattaac ttccgtattt 840

gatgctgatg ccggtatcgc attaaccgat actttcgtga agttagtatc ttggtatgat 900

aatgaaaccg gttattcaaa caaagtattg gacttagtag ctcacattta taactacaaa 960

ggctaa 966

<210>40

<211>1176

<212>DNA

<213> Actinobacillus succinogenes

<400>40

atgtcagtaa tcaaaatgac cgacctggat ttagcaggaa aacgtgtttt tattcgcgcc 60

gatttaaacg tgccggtaaa agacggtaaa gtggcgtccg atgcacgtat ccgcgcgact 120

atcccgacac ttaaacttgc gttggaaaaa ggcgcgaaag tgatggttac gtctcattta 180

ggtcgtccga ccgaaggcga attcaaaccg gaagattcat tacaaccggt ggttgattac 240

ttaaataatg cggagcttgg cgtgcctgtc cgcttagtgc aaaactatct ggacggggtg 300

gatgtgcaag caggcgaaat cgtggtgctt gaaaacgtac gcgttaacaa aggcgaaaag 360

aaaaacgatc ctgaattagg taaaaaatat gccgcacttt gcgacgtatt cgtcatggac 420

gctttcggta cggcacaccg tgcgcaggct tcgacttacg gcgtagcgga atatgcaccg 480

gttgcttgcg ccggtccgtt attagcggcg gaactagacg cattaggtaa agcgttaaaa 540

gaaccgcaac gtccgatgtt ggcgattgtg ggcggttcaa aagtttccac gaaattaact 600

gtgttggatt ctctttcaaa aattgcggat caattaatcg tgggcggcgg tatcgcgaat 660

acttttattg ctgcggaagg tcataatgtg ggtaaatctt tatatgaaga ggatttaatc 720

ccggaagcga aacgtttagc cgctgccact aacattccgg tgccggttga tgtacgcgtc 780

ggtacggaat tctccgaaag cgcaccggcg actgaaaaat ccgtgactga agtacgggcg 840

gacgaatcaa tctttgacat cggcgacaaa tccgctgaag aattagcgaa aatcatcaaa 900

tcggcgaaaa ctattctttg gaacggtccg gtgggggtat ttgaattccc gaacttccgt 960

aaagggactg aagtggtttc gaatgctatc gcagaagcaa cggcaaacgg cgcattctct 1020

atcgcaggcg gcggtgacac attagcggca atcgatttgt tcggtatcaa agataaaatt 1080

tcttatatct caaccggtgg cggcgcattt ttagaattcg tggaaggtaa agtcttgccg 1140

gcagttgaaa ttttagagaa acgtgcaaac ggttaa 1176

<210>41

<211>684

<212>DNA

<213> Actinobacillus succinogenes

<400>41

atgcaattag tatttattcg tcacggttta agcgaatgga atgcgttaaa ccttttcacc 60

ggctggagag acgtaaacct gagcgaaaaa ggcgtggaag aagccaaaga ggcggggcgt 120

aaattaaaag ccgccggttt cgaattcgac atcgcattta cttccgtatt aacccgtgcg 180

attaaaacct gtaacttagt gttggaagaa tccgaccaat tatgggtgcc gcaaatcaaa 240

acctggcgtt taaacgagcg tcactacggc ggtctgcaag gtttgaataa agcggaagcg 300

gcggccgaac acggcgacga acaagtccgt atctggcgtc gttcatatga tgtgttaccg 360

ccggttttgg atccgaaaga tccgaattca gcgcataacg accgtcgtta cgcacattta 420

ccggcggatg tggttccgga ttgcgaaaac ttaaaagtga cgctggatcg cgtattaccg 480

ttctgggaag accaaattgc accggcaatt aaagcgggta aacgcgtgct agtggcggcg 540

cacggtaact cgttgcgtgc attggcaaaa catatcgaag gtatttccga cgaagacatt 600

atgggtttgg aaattccgac cggtcaaccg ttagtatatg aattagacga taacctgaaa 660

gtgctcagta aacgttatct ttaa 684

<210>42

<211>645

<212>DNA

<213> Actinobacillus succinogenes

<400>42

atgaaaaaag atattcgatt ttatcttatc cgtcacggca gaacggtttg gaacgaacag 60

ggtttaatgc agggctgggg caactccgac ctgacggaag aaggcgtgaa aggagcaaaa 120

ctcacagggc tcgcattaaa tgaagtgccc tttgtcgcgg catattcaag ctgtctgcaa 180

cggactatcg atactgccca tcatatttta ggcgaacgga atattccgct ttttcagcat 240

aacggtttaa acgagcatta tttcggttca tgggaaggca cgcatgtgga atcaatccgt 300

caaacccggg aattccagca aatggtcgcg gatcctgtta actataaagc cttgacgaac 360

ggcggagaaa cttggcaaca attagccgag cgcactacga aagccattaa cgacattatt 420

cgaattcatg ataacggcaa tattttggta gtatctcacg gccataccgt gcgcttatta 480

atggctattt tcgccggcgc cacttggcaa accccaccgcg atcccggcat tagcgaatcg 540

atgaaaaata catccattaa tattgtgcat tatattcgaa acggactgtc ggattcaggg 600

cggtttattg tcgagaaagt gaatgatacc cgtcatttag aatga 645

<210>43

<211>891

<212>DNA

<213> Actinobacillus succinogenes

<400>43

tacggtactc cgggtcaata ttctatgccg ttaccgatga tgaacatcat caacggcggt 60

gaacacgcag acaacaacgt ggacattcaa gagttcatga ttcagccggt cggtgcgaaa 120

actttacgtg aggccgtacg tatcggttca gaagtcttcc acaacttggc taaagtatta 180

aaagctaaag gttataacac cgcagtcggt gacgaaggcg gttttgcgcc aaatttaggt 240

tcgaatgcgg aagctttggc gtgcattaaa gaagccgttg aaaaagcagg ctatacgtta 300

ggtaaagacg taactttagc gatggactgc gcgtcttccg aattctacaa caaagaaacc 360

ggtaaatacg aaatgaaagg tgaaggtcgt tcattcactt ctcaagaatt tactcactat 420

ttagaagaat tatgcaaaca atacccaatc gtgtctatcg aagacggtca ggacgaatcc 480

gactgggacg gttttgcgta tcaaactaaa gttttaggtg acaacgttca attagttggt 540

gacgattat tcgtgaccaa caccaaaatc ttgaaagaag gtatcgaaaa aggtatcgcg 600

aactctatct taatcaaatt caaccagatc ggttcattaa ccgaaacttt agccgcaatc 660

aaaatggcta aagacgcagg ttacacggcg gttatttcac accgcagcgg tgaaaccgaa 720

gatgcgacga ttgcagattt agcagtcggt actgcagcag gccaaatcaa aaccggttca 780

atgagccgtt cagaccgcgt tgcgaaatac aaccaattaa tccgtatcga agaagcatta 840

ggcgacaaag cgccgttctt agggttaaaa gcggtgaaag gtcaagctta a 891

<210>44

<211>1488

<212>DNA

<213> Actinobacillus succinogenes

<400>44

atgaaagcag aaaataattg tatcgtgatt ttcggcgcat caggggatct gacgcaccgt 60

aaattaattc ccgcactcta taatctttac aaaatcggac ggttggaaga aaacttctcc 120

gtgctgggag tggcccgcac agaaatgacg gatgacattt tccgtgaaaa aatgcgaacc 180

gccctgatta cccaagaaaa tgccgaaggc gaaacgctgg ataaattctg ttctcatctg 240

tattaccagg cggtaaacac ctccgattcg gcggattacg taaagttact gcctcgtttg 300

gatgaattac acgacaaata ccaaacctgc ggcaatacgc tttattatct atccactccg 360

ccgagcctgt acggcgttat tccggaatgc ctggcggctc acggcttaaa tacggaagaa 420

ttcggctgga aacggattat cgtggaaaaa ccgttcggtt acgatattaa aaccgccaaa 480

gcactggata ttcagattca ccgtttcttc gaggaacacc agattaccg tatcgaccat 540

tatttgggca aagaaaccgt gcaaaatctg ttggtgctgc gattctccaa cggcctgttc 600

gaaccgcttt ggaaccgtaa cttcatcgat tacgtagaaa tcaccggcgc ggaagagatc 660

ggcgtagaac aacggggcgg ctattatgac ggttccggtg caatgcggga tatgttccaa 720

aaccacttat tgcaagtatt ggcaatggtt gccatggaac cgccggcgat tattaacgcc 780

gattccatgc gtgacgaaac cgccaaagtg ctctattgtc tgcatccgtt gaccacggaa 840

gatctcaaac acaatctggt attagggcaa tacacggcct ccaccgttga cgataaaccg 900

gtgaagggtt atctggagga agcgggcgtg ccgtccgatt ccggcaccga aacctacatg 960

gcgttgcgct gccaaatcga taactggcgc tgggccggcg tgccgtttta cgtgcgcacc 1020

ggtaaacgcc tgccgacccg ggtgacggaa atcgtcattc atttcaaaac cacgccgcac 1080

ccggtattca gccaaaatgc gccggataac aaattaatca tccgtatcca accggacgaa 1140

ggcatttcca tgttcttcgg tttgaaaaaa ccgggagccg gcttcgaggc taaagaagta 1200

tccatggatt tccgttatgc ggatatcagt tcttccgcta atttattaac cgcctacgaa 1260

cgtttatgc ttgacgccat gaaaggcgac gccacattat tcgcccgtac cgacgccgtt 1320

cacgcctgct ggaaattcgt gcagccgatt ttggattaca aggaaaacca aggtcgcgtt 1380

tacgaatatg aagccggcac ctggggaccg gtggaagcgg ataaacttat cgcccgtgaa 1440

ggacgcgtat ggcgcagacc ttcgggctcc atgaagaaaa aagcgtaa 1488

<210>45

<211>1455

<212>DNA

<213> Actinobacillus succinogenes

<400>45

atgtcagaaa aaggcgacat cggcgttatc ggcttagcgg taatgggtca gaacttaatt 60

ttaaatatga acgacaacgg ctttaaagtc gtggcgttca accgtaccac ctcaaaagtg 120

gatgaatttt tacaaggtgc ggcgaaaggc acaaacatta tcggtgctta ttctctggaa 180

gatttagccg cgaaattgga aaaaccgcgc aaagtaatgt taatggtgcg tgccggtgat 240

gtggtggatc attttatcga tgctttatta ccgcatttgg aagcgggcga catcattatc 300

gacggcggta actcgaatta tccggatact aaccgccgca cacaagcgtt ggcggaaaaa 360

ggtgttcgct ttatcggcac cggcgtttcc ggcggtgaag aaggcgcgcg tcacggacct 420

tccatcatgc cgggcggtaa tcctgaagcc tggccttatg tgaaacctat tttgcaagcg 480

atttccgcca aaacggaca aggcgaacct tgctgcgatt gggtgggcaa agaaggcgcc 540

ggtcacttcg tgaaaatggt acataacggt atcgaatacg gcgatatgca gctcatctgc 600

gaagcttacc aattcttaaa agaaggtttg ggtttaagtt acgaagaaat gcatgaaatc 660

ttcaaacaat ggaaaacaac cgaactcgac agctatctgg tggacattac caccgatatt 720

ttggcataca aagatacgga cggtcagccg ctggtggaaa aaatcttaga caccgccggg 780

caaaaaggta ccggtaaatg gacggggatc aacgcgctag acttcggtat tccgttgaca 840

ttgattaccg aatccgtatt cgcccgttgc gtatcttcat tcaaacaaca acgagtcgaa 900

gcgtcaaaat tattcaacaa aaccgtttcg ccggttgaag gcgataaaaa agtgtggatt 960

gaagcggtac gcaaagcctt gctcgcctcc aaaatcattt cctacgccca aggcttcatg 1020

ttaatccgtg aagcatccga gcaattcgac tggaacatca actacggcgc gaccgctctt 1080

ttatggcgcg aaggctgtat catccgcagt gcatttttag gcaacattcg cgatgcttat 1140

gaagcgaacc cggatttagt gttcctaggt tccgacccgt atttcaaagg cattttagaa 1200

aatgctatgg cggattggcg taaagtggtg gcgaagtccg tcgaagtggg cattccgatg 1260

ccttgtatgg caagcgcgat tactttctta gacggttaca cttccgaacg cgtgccggcg 1320

aatttattgc aagcgcaacg cgactacttc ggcgcacaca cttacgaacg caccgacaaa 1380

ccgcgcggcg aattcttcca cactaactgg acgggtcgcg gcggtaacac ggcttccacc 1440

acttatgatg tgtag 1455

<210>46

<211>702

<212>DNA

<213> Actinobacillus succinogenes

<400>46

atgaactaca ttaccttccc gaccgcccga catgccgtgg aaaaaatcgc acaggaattc 60

gtgctttaca gccaattaaa ccgtccggtg catatttccc tttccggcgg ctcgacgccg 120

aaacttttgt tcaaaacctt ggctcaagca ccgtttaaca ccgatgttcg gtggcaaaat 180

ctgcattttt ggtggggcga cgacagaatg gttccgccga caaatcccga aagcaactac 240

ggcgaagtac agaaactgct gttcgatcac attcaaattc cggcgcaaaa tattcaccgt 300

attcgcggcg aagaaccggc ggaacaggaa ctgaaacgct ttgaagcgga attaagtgcg 360

gtcgttcctg acggtgtttt tgactggatt attctcggca tgggcggcga cggccatacg 420

gcgtctttat tcccgcatca aaccgatttt aacgatccga atcttgccgt aatcgccaaa 480

cacccggaaa gcggacaaat ccgcatttcc aaaaccgcta aactgatcga acgggcaaaa 540

cgcattacct atctggttac cggtgcggca aaagcggaaa tactaaaaca aatccaaact 600

accccggcgg aacaactgcc atatcctgtc gctcgcattc aggcaaaaaa cggcataacg 660

gaatggtatt tggatgcgga tgcggcaaag ctattaaact ag 702

<210>47

<211>1455

<212>DNA

<213> Actinobacillus succinogenes

<400>47

atgtcagaaa aaggcgacat cggcgttatc ggcttagcgg taatgggtca gaacttaatt 60

ttaaatatga acgacaacgg ctttaaagtc gtggcgttca accgtaccac ctcaaaagtg 120

gatgaatttt tacaaggtgc ggcgaaaggc acaaacatta tcggtgctta ttctctggaa 180

gatttagccg cgaaattgga aaaaccgcgc aaagtaatgt taatggtgcg tgccggtgat 240

gtggtggatc attttatcga tgctttatta ccgcatttgg aagcgggcga catcattatc 300

gacggcggta actcgaatta tccggatact aaccgccgca cacaagcgtt ggcggaaaaa 360

ggtgttcgct ttatcggcac cggcgtttcc ggcggtgaag aaggcgcgcg tcacggacct 420

tccatcatgc cgggcggtaa tcctgaagcc tggccttatg tgaaacctat tttgcaagcg 480

atttccgcca aaacggaca aggcgaacct tgctgcgatt gggtgggcaa agaaggcgcc 540

ggtcacttcg tgaaaatggt acataacggt atcgaatacg gcgatatgca gctcatctgc 600

gaagcttacc aattcttaaa agaaggtttg ggtttaagtt acgaagaaat gcatgaaatc 660

ttcaaacaat ggaaaacaac cgaactcgac agctatctgg tggacattac caccgatatt 720

ttggcataca aagatacgga cggtcagccg ctggtggaaa aaatcttaga caccgccggg 780

caaaaaggta ccggtaaatg gacggggatc aacgcgctag acttcggtat tccgttgaca 840

ttgattaccg aatccgtatt cgcccgttgc gtatcttcat tcaaacaaca acgagtcgaa 900

gcgtcaaaat tattcaacaa aaccgtttcg ccggttgaag gcgataaaaa agtgtggatt 960

gaagcggtac gcaaagcctt gctcgcctcc aaaatcattt cctacgccca aggcttcatg 1020

ttaatccgtg aagcatccga gcaattcgac tggaacatca actacggcgc gaccgctctt 1080

ttatggcgcg aaggctgtat catccgcagt gcatttttag gcaacattcg cgatgcttat 1140

gaagcgaacc cggatttagt gttcctaggt tccgacccgt atttcaaagg cattttagaa 1200

aatgctatgg cggattggcg taaagtggtg gcgaagtccg tcgaagtggg cattccgatg 1260

ccttgtatgg caagcgcgat tactttctta gacggttaca cttccgaacg cgtgccggcg 1320

aatttattgc aagcgcaacg cgactacttc ggcgcacaca cttacgaacg caccgacaaa 1380

ccgcgcggcg aattcttcca cactaactgg acgggtcgcg gcggtaacac ggcttccacc 1440

acttatgatg tgtag 1455

<210>48

<211>453

<212>DNA

<213> Actinobacillus succinogenes

<400>48

atgaaaattg ctatcggatg tgatgatgcc gcatataact taaaaattga gttaatcaaa 60

tatctggaaa cattaggtat tgaatgcgat gactttggcg ccggtgcggg cgatttgaca 120

ttgtatccgg atgttgccga gaaagtagct ttggcggttt ccgaaggcaa atatgagcgt 180

ggaattctta cctgcggaac cggtatcggc atgtgcatta cggcaaataa agttccgggg 240

gtaagagcgg cggtttgtca tgacgttttt tctgccgaac gttcaagaaa aagtaatgat 300

gcgcaaatta tctgtttcgg tgagcgtgtg attggcgtgg aattagcaaa atcactactt 360

aaagtgtggt tggaatgtga ttttgcaggc ggtggctcaa caccaaaatt agcaaaaatt 420

aaagaaattg atgctaaata taataaacgt tag 453

<210>49

<211>450

<212>DNA

<213> Actinobacillus succinogenes

<400>49

atgaaaattg caattggatg tgatgaagcg gcatatcgtt taaaagttga aattatgaaa 60

catttagatg ctattggcgt tgaatatgac gatttcggcg caggcgaggg cgatgtggtg 120

ctataccccg atgttgcgga agccgtagcc gtcgctgtgg cggaaggcaa atatgaacgt 180

gccattttaa cctgcggtac ggggatcggc atgtgcatta cagcgaataa agtaccaggt 240

gtgcgggctg cggtctgtca cgacgtattt tctacggaac gttcgcgcaa aagtaatgat 300

gcgcaaatta tgtgcttagg cgaacgtgta atcggtgtcg aattagccaa atctctgatt 360

gatgtttggt ttaaatgtga ttttgccggc ggcggttccg caccgaaagt tgcacgtatc 420

aatgaaattg atgcgaaata ccacaaataa 450

<210>50

<211>642

<212>DNA

<213> Actinobacillus succinogenes

<400>50

atgaaaattg cattaatgat ggaaaacagc caagcggcaa aaaatgccgt agtgttaaaa 60

gagctgaaag atgttgtgga ttctaaaggt tattcggtat ttaatgtcgg aatggctaat 120

gaagacgacc atcacttaac ttatattcat ttaggtatta tggcaagtat tttgcttaac 180

tctaaagccg tggactttgt ggtaaccggc tgcggcacag gacagggggc aatgatgtcg 240

cttaatttac atcccggtgt tgtatgcgga tattgccttg atccggcgga tgcgttttta 300

ttctgtcaga ttaataacgg taatgcgctt gctttggcct ttgccaaagg ttttggctgg 360

ggcgccgaat taaacgttcg ctacatgttt gaaaaagctt ttaccggcat tcgcggtgaa 420

ggttatccgg ttgaccgcaa agaaccgcag gtacgtaatg cgggtatttt gaatgaagtg 480

aaaaaagcgg tagcgaaaga taattacttg gacactttac gtgcaattga ccctgaattg 540

gtgaaaactg cagtcagcgg cgaacgtttc caacaatgtt tcttcgaaaa ttgtcaggat 600

aaagaaatcg aagcctttgt tcgtgaagta ttagcaagat ag 642

<210>51

<211>660

<212>DNA

<213> Actinobacillus succinogenes

<400>51

atggatcaac tcgaaatgaa aaaaatggct gctcaagccg cattacaatt tgtgcaaccc 60

gtagtatcg tcggggtcgg cagcggttct accgtaaatt gtttcattga agcgctgggt 120

gcaatgcggg atcaaatcaa aggtgcggtg gcggcatcca aagcgtcgga ggaattgctg 180

cgcaaacagg gcattgaagt gttcagtgcc aatgatgtgt cgagtttgga tattacgtg 240

gacggtgcgg acgaaatcaa tccgcagaaa atgatgatca aaggcggcgg agcggcgtta 300

acccgcgaga aaatcgtagc gtcgctgacc aaaaatttta tctgtattgt ggacagttca 360

aaacaggtgg acgtgctggg ttccacattt ccgttaccgg tggaagtgat tcctatggcg 420

cgttctcaag tggcacgtaa actggtcgca ttaggcggtt cgccggaatg gcgtcagggg 480

gtggtgacgg ataacggcaa tgtcatttta gacgtacata atttcccgat tatgaatccg 540

ccggaaatgg aaaaggaatt gaataacgtg gcgggcgtgg tgacaaacgg cattttcgcc 600

ttgaatgcgg cgaataccgt gattgtcggc acgcctgaag gtgcgaaaat catcaaataa 660

<210>52

<211>831

<212>DNA

<213> Actinobacillus succinogenes

<400>52

atgaacccgt ataatttgtc ctatgatgag ttagaaaaaa aagccaaagc aattcgtcgg 60

aaaatcgtcg tattaaatgc taacagtcct gctggtggtc atacaggcgc agattatctct 120

caagtagaaa tcttaacttc attgtacttc cgtgtattaa ataatgatcc aaaagatta 180

attaacccag agcgtgatat ttatattcag tctaaaggtc atggtgcagg tggttattat 240

tgctgtttag ccgaggcagg ctatattcct gaagactggt taccaaccta tcaacattca 300

gattcaaaac ttccaggcca cccagtaaaa cataaaacac ctggtgtgga attaaatacg 360

ggggctttag gtcatggttt acctgtggct gtcggtttag ccattgccgc taaaaaaagc 420

ggtagtaaac gtaaaatcta tgtgttaacg ggggatggcg aacttggcga gggtagtaac 480

tgggaagctg cattgactgc ggcacaatat aaattagaca accttattat tattaatgat 540

aaaaataaat tacaacttgc tggttttact aaagatattt tatgtactga tccattagat 600

aaaaaatggg aagcgtttgg tatggaagtg catgaatgcc aaggtaatga tattcgttca 660

gtcgtagata ccttagaaag cattcagcca aatggcaaac cacatgtagt tattgctaat 720

actacgaaag gtgcaggtat ttcatttatt gaaggtcgtc cagaatggca tcacaaagta 780

ccgaaaggcg atgaagttga attagcatta gaggagttga aagatgagta a 831

<210>53

<211>936

<212>DNA

<213> Actinobacillus succinogenes

<400>53

atgagtaacg cagaacattt agcaaatatc atggtggaac gctttataag tgcggtcaaa 60

aatggcgtag atttagttcc tgtagttgct gattcaacat caacagcaaa aattgctcca 120

tttattaaag aatttccaga tcgcttagtg aatgttggta ttgctgaaca atctttggtt 180

ggttgtgctg cagggttagc tttaggtggt aaggtggctg ttacttgtaa tgcggcacca 240

ttcctaattt cacgagccaa tgagcaagtg aaagtggatg tttgctacaa caacacaaat 300

gtaaaactct ttggattaaa ttcaggtgcc agctatggtc cattagccag cacgcaccac 360

agtattgatg atattggagt gatgcgtggt tttggtaata tccagatctt tgcgccatcc 420

agtccaaatg aatgtcgcca aataattgat tatgcaatta attatgtcgg tccagtgtat 480

attcgccttg atggtaaaga attaccagag atccataatg atgactatga gtttgttcca 540

ggtcaaatcg atgtattacg gaaagggggt aaaattgcgt tagtggctat gggttctaca 600

gtatatgaaa ttgtagatgc ggcagttaaa ttagcagaaa aaggcattga agtcaccgtt 660

gtaaatgtgc cttcaattcg cccatgtgat acagaggcat tattcaatgc aattaaagat 720

tgtaaatatg tgattagtgt tgaagaacat aatattaacg gtggtgtcgg tagtctagtg 780

gctgaagtga ttgctgaaca tggtgcaccg attacgttaa aacgccgagg tattgcggat 840

ggtggttatg cactggcagg tgatagaaaa tcaatgcgta aacaccatgg tattgatgcg 900

gatagcattg tggacttagc cttatcttta aactag 936

<210>54

<211>2007

<212>DNA

<213> Actinobacillus succinogenes

<400>54

atggcagatc aaaaacaatt agcaaatgcg attcgcgcat taagtatgga tgctgttcaa 60

aaagcaaaat ccggtcaccc gggtgcccct atgggaatgg cggatattgc cgaagtatta 120

tggcgtggtt ttatgaaaca caatccgagc aatccgaaat gggcggatcg cgaccgtttc 180

gtgctttcca acggtcacgg ttctatgttg atttacagtc ttttgcattt aaccggttat 240

gatctttcca ttgaagattt aaaaaacttc cgtcagcttc attctaaaac gccgggacat 300

ccggaatacg gctatgcacc gggcgtcgaa accacaaccg gtccgcttgg tcaaggtatt 360

accaatgccg tcggtatggc gattgcggaa aaaaccttag cggcgcaatt caaccgcgaa 420

gggcatgaca tcgttgatca ccaacacttac gtgttcttag gcgacggctg tttaatggaa 480

ggcgtatccc atgaagcctg ctcgttggcg ggaaccttgg gactaggaaa attaatcgcg 540

ttttacgatg acaacaatat ttctattgac ggtcatgtgg acggctggtt caccgataac 600

acgaaaggtc gtttcgaagc ttacggctgg caagttatcg acaatgtcga cggtcacgat 660

ccggaacaaa ttgctgctgc agtgaaattg gcgcaagcgg aaacagaaaa gccgtccatt 720

attatttgca aaaccatcat cggttacggt tcacctaata aatccgcctc tcatgattgt 780

cacggcgcgc cgttgggtga agaagaaatc gcattaacgc gcaaagcgtt aggttggaat 840

tatgcgccgt ttgaaattcc ggcggatatt tacgctgcat gggatgccaa agcagccggt 900

caggcggcag aagcggcatg gaatgaaaaa tttgccgctt atgccaaagc ttacccggaa 960

ttagccactg aattcactcg ccgtgtcaac ggtgaattgc cggcaaactg ggcggcggaa 1020

tctcaggctt tcattgaaaa attacaagct aatccggcag ccattgccag ccgtaaagct 1080

tcacaaaatg ctatcgaagc ttatgcccgt attttacctg aatttttagg cggttcggcc 1140

gacttggcaa gttctaatct cactttatgg tcgggttcga aaccgattcg tgcgaaagaa 1200

aatacggatg gtaattacat caactacggc gtacgtgaat tcggtatgtc cgccattatg 1260

aacggtatcg ccttacacgg cggcttcatt ccttacggtg caactttcct aatgttctac 1320

gaatacgctc acaatgcagt gcgtatggcg gcattaatga aacaacgtgc attattcgtt 1380

tatacccatg attccatcgg cttgggggaa gacggcccga ctcaccaacc ggtagagcaa 1440

acggcggcat tacgtttaat tcctaatctg aacgtatggc gtccggcaga ccaagtagaa 1500

tccgccgttg cctggaaaga ggcggttgaa cgtcaagacg gcccgagcgc attaatcttc 1560

acccgtcaaa acttagcgca acaagaccgc acttccgccc aattagccaa cgtggcacgc 1620

ggcggttaca ttttgcgcga atgttgtgaa aaaggcgatt gtccggattt aatcctgatc 1680

gccaccggtt ccgaagtgga attagccatg aaagcggctg acgtattgga tgctgaaggc 1740

gtaaaagttc gtgtcgtgtc aatgccgagc actaatgtat tcgacaaaca ggacgaagca 1800

taccgcgaat ccgttttgcc gagctccgtc accaagcgtg ttgctatcga agcacaaatt 1860

gccgatttct ggtataaata cgtcggtctt gaaggccgta ttatcggtat gaatcgtttt 1920

ggcgaatccg ctccggcaga gcaactattc aaactcttcg gttttacggt agaaaacgta 1980

gtagcgaaag ccaaagaaat tctttaa 2007

<210>55

<211>666

<212>DNA

<213> Actinobacillus succinogenes

<400>55

atgcaactct atctcgatac ggcaaacctc gaaaccatta aaaaatttca ccgcacttta 60

ccgctgcaag gcgtaaccac caatcccagt attctggcga aagagaaaca gcctgtagct 120

gaaactttgc aggcgattca acagattatc ggcgaaaacg gtcagctttt tgctcagaca 180

ctaggccgtt ccgcccggga aatgatagat gacgccttgg cgttacggga aatcgtaccg 240

actatcgcgg ttaaaatccc cgttactccc gaaggtttgg tcgctattaa ggagttaacc 300

cgattgagcg tacccgtttt gggaaccgcg gtatacggtg cggcgcaagg atttttagca 360

gccttggccg gcgccgtata tatcgcccct tatgtgaacc gtatcgatgc acagggcggc 420

agcggtatcg atgtggtacg ggaattacag gcgttactgg atcaacacgc accggattct 480

ttgatttgcg cggcaagttt ccggacgccg cgccaagctt tagactgttt actggccggt 540

tgtcagtcca tcacgttgcc gcctgatatt gcgctggctt tcttgaatga tccggcggta 600

tttgccgccg tggaaaaatt tgaagcggac tggcaaaatg cgtatcaacg cagtacattg 660

ctgtaa 666

<210>56

<211>954

<212>DNA

<213> Actinobacillus succinogenes

<400>56

atgacgacac aattagatgc attaaaacaa atgaccgtgg tggttgcgga taccggcgat 60

attgaagcga tgaaattgta taaaccgcaa gatgccacca ccaatccttc tttaatttta 120

agcgcttccg cactgccgca atacgtctca ctaatcgatg atgccgttgc gtacgctaaa 180

gctaaaagtt cggacaaagc acaacaatta atcgatgcgg aagataaact tgcggtgaat 240

atcggtttgg agatcctaaa actcgtaccg ggtcgtattt ctacagaagt agatgcccgt 300

ctttcttacg acattcaggg gacaattgaa aaagctcgta aaattattgc gttatataac 360

gaggcgggtg ttgccaacga tcgcattctg attaaagtcg cttcaacctg gcagggtatc 420

cgggcggcgg aagtacttga aaaagaaggc attaactgca acttaacgtt attattctct 480

caagcccaag cccgcgcctg tgcggaagcc ggcgtttatttgatttcacc gttcgtcggt 540

cgtattcttg attggtataa agccaacagc gacaaacaag aatatgcacc ggcagaagat 600

ccgggtgtaa tttccgtgac gcaaatttac aattactata aacaatacgg ctataaaact 660

gtcgtaatgg gtgcgagttt ccgcaatatc ggcgaaatta ccgaattggc aggttgcgac 720

cgtttaacca tcgctccgcc attackgaaa cagttacagg aaaacgaggc tccgttagcc 780

cgtaaattgg aatataaagg cgaagtacaa actcggcctg ccccaatgac agaagcggaa 840

ttctactggg aacataatgc cgatccgatg gcggtggaaa aattagcgga agggatcgt 900

aaatttgccg cggatattga aaaattagaa gcaatgcttg cagcaaaact ctaa 954

<210>57

<211>816

<212>DNA

<213> Actinobacillus succinogenes

<400>57

gtggaaatta tcaaaattgc cataccggac ggtgaaaaaa cattaaatgc tattgataat 60

ttagctgcta atggggcgaa aggatttgtt atttgtacgc cggaccccaa gctgggtccc 120

gctattatgg cgaaagcccg ttctcatgat ctaaaagtga ttgcggttga tgaccaattc 180

ttaaatgcag cgggcgagcc gatgactaat gtgcctttaa ttatgatggc ggcgacggaa 240

atcggtcaac ggcaaggtga agaactttat aaagaaatgc aaaaccgcaa atgggatgtg 300

aaagatacag cggttttagc tatcaccgcc gatgaactgg ataccgcacg tcgccgcacc 360

gaaggctcta tggacgcctt aatcaaagca ggtttcccta aagaaaaaat ttataaatct 420

ccgaccaaaa gtaacgatat tccgggcgca cttgatgcgg cgaattctat gttggtacag 480

cacccggaag taagaaactg gcttatcgtc ggtatgaacg ataacaccgt attaggcggc 540

gtgcgtgcga ccgagggaca aggtttaaa ccggaaaacg tagtggctat cggtatcaac 600

ggcgtagatg cggttaacga actttccaaa ccgaaagcta ccggtttttt aggttctctt 660

ttgccgagtc ccgatattca cggttatcgc agcgtcgagc tcctcagtaa atggattaga 720

gaaggtgtgg aaccggaaaa atacattgca gttcaggacg ttgtattcct gaagcgtgaa 780

aatttcaaag aagaactggc taaaaaaggt ctttaa 816

<210>58

<211>1503

<212>DNA

<213> Actinobacillus succinogenes

<400>58

atgcaagttc cgtatttaga atttgataat gtgagtaaaa gctttccggg tgttaaagca 60

ttacagaata tttcctttaa atgctacgaa ggcagggttc atgcgctgat gggggaaaat 120

ggtgcgggta agtccacgtt gctgaaaatt ttaagcggta actatttacc gagcgaaggc 180

aaattaagta tcggcggacg tcagttggtt tttcgtaata ccaaggaagc attattggcc 240

ggagtggcca ttattacca ggaattgaat atcgtgccgg aaatgacggt agccgaaaat 300

ctgtgtttag gtcagtttcc ccattctttc ggaatcgtcg ataaatcgga attggtaaaa 360

agaacacaga aatatcttga taaattagat ttaaatattt cacccgacac ccctttaaaa 420

gaattatcta tcggtcagtg gcaaatgatt gaaattgcca aagcattgag ccgaggagca 480

aagattattg ctttcgacga gccgaccagc agtctttccg cacctgagat tgagaaatta 540

ttttcggtaa ttaatgagtt acgggatgaa ggcaaagtga ttctgtatgt ttctcaccgt 600

atggaagaaa ttttccgtat tagtgatgaa attaccgtgt taaaagacgg gcaatatgtg 660

gaaactttct cttctctctc caaaatcacc aatgacgatc tggttcgcag catggtagga 720

cgtaatttag gcgacattta tcatttatcgt ccccgtgatg taggaaaaga gcggttaaaa 780

atcagccgct tgtccggaca taaactgaaa ggcgacttta acttaaccgt tcgggcgggc 840

gaagtacttg gtttattcgg tttggtgggc gccgggcgtt cggaactgtt aaaagtgatt 900

ttcggcgccg atccgaatat tgaaggtaca atcgaattgg acggtaaaac cgtttgtata 960

cgcaatccga aagaagcgat agaacagggg attgtgcttt gtcccgaaga tcgtaaaaaa 1020

gaggggatta ttccgacggc aaccgtagcg gaaaatatca atatcagtgc gagacgttcc 1080

cataattttt tcaaatttt tattaacgaa aagtgggaaa aagaaaatgc cgaacgacaa 1140

cgccggcaaa tgaatgtaaa aaccccgtcc attcaacaac ttaattgttaa tttgtccggt 1200

ggtaaccaac agaaagccat cttgggacgt tggttgtccg aagacattaa agttttattg 1260

ttggatgaac cgacgcgagg tattgatgtc ggggcaaaat cggaaattta cgatttgatt 1320

tttaaacttg ccgatgaaaa actcgccatt attgtggtat ccagcgactt accggaagta 1380

atcggcgtat cggatcgaat tatggtaatg cgtggtcatc aaattaccgg cgtagtaacg 1440

cgtgaagagg ccacggaaga aaaaatatta aaacttgcta tggtagaaag taaagcggct 1500

taa 1503

<210>59

<211>969

<212>DNA

<213> Actinobacillus succinogenes

<400>59

atgacttcaa taactcagga aaaaacagtg ggttcatttg gtagaatttg gaactcttac 60

ggcatgctcc ttatttttgc cgtgattttt atcggttctt gtttattcat tcctaatttt 120

gctaccgtcg tcaatatgag gggcttaggg ttggccattt ctatgagcgg gattgttgcc 180

tgcggtatgt tgttctgttt ggcggcgggg gaaatcgatc tttccgtggc ttccgttatc 240

gcctgtgccg gggtcgtgac ggcggtagcg attaatatga cgcaaagcgt tacggtcggt 300

attcttgccg gtctcggtct gggtgttgcg gtcggattaa ttaacggttt tgtcattgca 360

aaactaaaaa tcaactcatt aatcactaca cttgcgacca tgcagatcgc ccgcggtttc 420

ggctatatta tttcggacgg taaagcggtg gggattacca aagaagaatt cttcgaactg 480

ggttaccagg atattttcgg tgtgccttta ccgatcattt ttactgttat ctgtatcgcc 540

gttttcggtt ttttactcag taaaaccact tacggacgta atactctggc gataggcggt 600

aatcaggaag cgtcccgttt ggcgggtatc aatgtggaca gaacaaagtt aattattttt 660

gtggtatccg gttttgtttc cgctctcgcc ggcgttattt tggccgcccg tatgaccagc 720

ggtcaaccga tgacatccgt ggggtttgaa ctggtggcga tttcggcttg tgtgctgggc 780

ggcgtttcgc tgaacggcgg agtggcgaaa atttcattta tcatcgccgg cgtgttaatt 840

ctcggcacta ttgaaaatgc catgaaccta ttaaatatct cgccgttcgc gcaatatgtg 900

gtgaggggcc tcatcttgtt gattgcggtt atttttgata aatataaaca gaaaattatc 960

aaatcttaa 969

<210>60

<211>1488

<212>DNA

<213> Actinobacillus succinogenes

<400>60

atggaattta ttaaaaaact tgaagtctgg tttatagtag gcagccaaga tttatacggc 60

gatgaagcgc tgaaacaagt gtatgccaat gcgaaacaaa ttacgcaata tttaaacagt 120

cggaatccgt ttatcagat caaattaaaa ccgttagcca ccacaccgga agacattctt 180

gctatttgcc gggcggcaaa ttatgaggaa aactgtgccg gagtcatcgc ttggatgcat 240

accttctctc ctgccaaaat gtggattgca ggcttaaccc gattagacaa accgctatta 300

caattccata cccagttcaa taaatatatt ccgtggaatg aaattgatat ggattatatg 360

aatttacacc agaccgccca cggcgatcgt gaattcggtt tcatggtttc ccgtctgcgt 420

aaaccgcgaa ccatagttgt agggcattgg caaagcgaat cggttaaaca aaaattggat 480

cgctggatgc gcgtattggc ggccattat gatcaacaac atctgaaagt cgcccgcttt 540

ggcgataata tgcgagaagt cgccgtaacc gaaggggata aagttgaagc acagatcaaa 600

ttcggttaca gcgtaaacgg ttacggcata tatcaactgg tgaacagtat taatgcgatt 660

aaagaggaag atatagccac cttggtgaaa gaatatgaag ctgattatca actggtggac 720

agtctgaaag aaggcggtga gaagcgccaa tcactgattg attccgcccg catcgaatta 780

ggtttaaaag cattcttaga taaaggcgga ttcaaagctt tcaccgacac tttccaaaat 840

ctatacggta tgaaacagct tccgggatta ccggttcagc gcttaatggc acagggttac 900

ggtttcggtg cggaaggcga ttggaaaacc gcagcgttag tacgggcgat aaaagtcatg 960

agctacggtt taccgaacgg ttgctcgttt atggaagatt acacctataa tctcgaagat 1020

cataacgaag tggtgcttgc cgcacatatg ctggaagtct gtccgtcaat cgccgaagac 1080

aaaccgattc tcgacataaa accgttaggt attggcggta aagaagaccc gacccgtctg 1140

attttcacgt ctaaagcggg ctgtgccacc gcttcgacta ttgtggattt ggggaaccgc 1200

ttccgaatga ttacggcgga attgcaagcc attgccaaac cgcaggatat gccgaattta 1260

ccggtcggtc atgcattctg gaaactggaa ccggatttcg atacgggtac gcaggcttgg 1320

attttagccg gcggcgcaca ccacaatgta ttcagtttgg atattgatgc ggacatgctc 1380

agaacttttg cggaatattt cggaattgaa tttatcata tccatgccaa aacggaattg 1440

gctgatttga aaaatgaact gcgttggaat gacgtagctt ataaatag 1488

<210>61

<211>1458

<212>DNA

<213> Actinobacillus succinogenes

<400>61

atgaattact accttggcat agattgcggc ggtacctttg tcaaagccgc tttatttgat 60

gaaaccggca acttacaggg tatcgcccgc gaaaacgtac cggtgatgag cgataaagcc 120

ggttacgcgg aacgggatat gccgcagctt tggcaagtgt gcgctgacgt agtacgccaa 180

actattgccg agagtaatat ttcgcccgat tttatcaagg gggtcggtat ttcggcgcaa 240

ggcaagggcg catttttatt agataagaat aaaaaacctt taggtcgcgc gattttatct 300

tccgatcaac gttcgttaga cattgtcaaa caatggcaac aaaaaggcat tcccgaaaaa 360

ctctatccgc tgacccgcca aaccttatgg acgggacatc ctgtttctat tttgcgttgg 420

ctaaaagaaa acgaacccga ccgttacgcc cgtatcggtt cggtactgat gtcccacgat 480

tatcttcgtt tttgcctaac cggcgagctg cattgcgaag aaaccaatat ttcagaatcc 540

aatctctaca atatggaaaa agggagatat gacccggttt tagcggattt attaggtttg 600

gaaggcataa ttgaaaaatt accgccggta atccggtccg atcaaatcgc cggctatgtg 660

acgcaacaag ccgccgagct gaccggtttg aaggcgggca ctgcagtggc gggcggatta 720

ttcgatgtgg tatccaccgc gctgtgcgcc ggtttggaag acgaaaccaa actgaacacg 780

gttttcggta cctggtgtgt ggtgagcggc attaccgatc acatcgcggc caatcaatcg 840

ctgcccttcg tttacggtcg ttatgcggaa aaaaacaaat ttatcgtaca cgaagccagt 900

ccgacgtcag ccggtaattt agaatggttc gtcaaacaat ggaatctgga ttacggacaa 960

attaacgaag aaatcgccgc actgccgccc gccgccagtt ccgtcttatt cgtaccgttt 1020

ttatacggtt ccaatgccgg tttgggcatg caggcaggat tttacggtat tcaatctcat 1080

cacaaacagg cgcatttgtt gcaagccgtt tatgaaggcg ttttgttcag cttgatgtat 1140

catctggaca gaatcttaca acgcttccct cacaccaatg tattacgggt gacgggcggt 1200

tccgccaaat cggcggtttg gatgcaaatg ctggcggatt tcaccggcat gaccttggaa 1260

atacccaaag tggaagaaac cggctgtctc ggtgcggcag cgattgccat gcagtcatcc 1320

ggcgttacgc tgaacttcag caaagttctg aataaaggca tgaccgtagt tcaaccgaat 1380

ccggataatt atgccgctta tcagcaaaaa tatcggcgtt atgtgaaatt cactacggcg 1440

ttaaaagcga tgttgtga 1458

<210>62

<211>1455

<212>DNA

<213> Actinobacillus succinogenes

<400>62

atgaattatt atttaggcat cgactgcggc ggcactttta tcaaagccgc cctgttcgac 60

gaaaacggca atattcaggc ttgcgagcgt gaaaacgtca ccgtcgtcag caagcaatcc 120

ggctacgccg aacgggatat ggacgagcta tggcaaatgt gtacgaaggt gattcggcgc 180

accctaaaat caagcaaaat ccgaccgcac ttgatcaaaa gcgtcggcat ttcggcaaa 240

ggcaaaggcg cattcttact ggataaacac aaccgcccgt taggacgggc gattttgtct 300

tccgatcagc gtgcgcaggc tatcgtcaaa cgctggcagg cggaagggtt ggaagagaaa 360

gtctatccgc tgtcccgcca aggattgtgg acgggtcatc ccgttgccat tttacgctgg 420

ttgaaagaaa acgaaccggc acgttaccgc catatcggca atttgctgat gtcccacgat 480

tatctgcgtt tttgtctgac cggtaacctg tattgcgaag agagcaatat atccgaaagc 540

aatttatata atatgattac ggggaaatac gaccctcgac tggcacaatt gctcggtatt 600

gaagaaatca cggagaaact gccgcccgtc attaaagcga atcaaatcgc cggttatgtt 660

acggaacaag cggcgcaagc ctgcgggctg gctgtgggta cgccggtggt cggcggtgta 720

ttcgacgtta cggcgatgac attatgcctt acggataatc aacccggcaa actgaatgta 780

gtgctgggaa cttggtctat cgtaaccggt atttccgatt cccttgacga cagactggat 840

tcgcgttttg cccacgggcg ttatgtagaa gcgggcaaat tcctgattca ggaggccagt 900

ccgacttccg ccggcaatct ggaatggttc gtgaaacagt ggaatctgga ttatcagcaa 960

atcaatcaaa tgattgctcc gcttccgccg gcgcaaagta cggtcgtttt tctgccgttt 1020

ttatacggta ccaatgccaa actgggtatg accgccggtt tctacggtat gcaggcgcac 1080

catacgcagg cgcatttgtt acaagcggtc tacgagggcg tgctgttcag tctgatgaca 1140

catctagagc gtatgtatcg acgctttccg caaatacaga cattacgcgt gaccggcgga 1200

ccggcgaaat ccgccgtctg gttgcaaatg ctggcggatc tgacgggtaa aatactggaa 1260

gtaccggaag tggaggaaat cggttgtctg ggcgccgcat taatggcggc ggaaggcgtc 1320

ggcgctaacg gcagcgcatt aaaacgacat caggcgatac gtgttattcg gccgaatcct 1380

gccaatttcg acgcttaccg aaacaaatac cggcaatatc aaaaactggt ggagttatta 1440

gcgcagatga cgtaa 1455

<210>63

<211>693

<212>DNA

<213> Actinobacillus succinogenes

<400>63

atgttaaaag agttaagaga acgagtgcta caggctaatc ttgagttgcc gaaacacaaa 60

ttaattacct ttacctgggg taacgtcagt gaaatcgatc gcgaaaagg cttggtcgct 120

attaaacctt ccggtgtaga ttatgatgtg atgacggtgg acgacatcgt gattgtggat 180

ttagagggca atcacgtgtg gggtgataag aaaccttcgt cggatacggc gacacatctg 240

gaactttatc gccaatttcc tgaaatcggc ggtatcgtgc atactcattc ccgtcatgct 300

accgcttggg cgcaggcggg cgaagatttg atcgcattag gcacgactca cggcgactat 360

ttctacggtg ccattccctg tacccgtaaa atgacgccgg aagaaattgc cggcgaatac 420

gaactggaaa ccggcaaagt gattgtggaa actttccgca ctcgcaacat taatccgcag 480

gatattccgg cggtattggt aaattcccac ggtccgttcg tatggggtaa agacggtttc 540

aacgcggtac ataattcggt agtgctggaa gagattgcgt acatgaacgc attcagcaaa 600

ttaatccgtc cgaacgttca gccgatgcaa caggaattgc tcgataaaca ttacctgcgc 660

aaacacggta aaaatgctta ttacggtcag taa 693

<210>64

<211>1314

<212>DNA

<213> Actinobacillus succinogenes

<400>64

atgacaaact acttcgacaa aattgaaaaa gtgaactatg aaggtgcgga ttcgattaat 60

ccgtttgcat ataaatacta caacgcgaat gaagtgatct taggcaaaac catggctgat 120

catttacgtt tggctgtctg ttactggcat actttctgct ggaacggtaa cgatatgttc 180

gggtcggtt ctctggatcg cagttggcaa aaacaaaccg atccgcttgc cggagcaaaa 240

caaaaagcgg atatagcatt tgaattttta actaaattag gggtgcctta ttaattgtttc 300

catgatgtgg atatcgcacc cgaaggcaat tcttatcaag aatatgtgaa taatttcaac 360

acgattgtgg atgtcttaga acaaaaacaa gcggaaaccg gttattaaatt gttatgggga 420

acggcgaact gtttcactaa tccgcgttat atgtccgggg cggcaaccaa tccgaaccct 480

gaaatttttg cccgtgcagg tgcgcaagta tttaacgcaa tgaacgcaac caaacgttta 540

ggcggtgaaa actatgtatt gtggggcggt cgggaaggtt acgaaacctt gttaaatacg 600

gatttacgtc gtgagcgcga gcaaatcggt cgtttcatgc aaatggtagt ggaacataaa 660

cataaaatcg gtttcaaggg gacatttatta atcgaaccca aaccgcagga accgaccaag 720

catcaatatg actacgatgt ggcgacggtt tacggtttct taaaacaatt cggtttggaa 780

aaagaaatca aggttaacat tgaagcgaat cacgcaacct tggcggggca tactttccag 840

catgaaatcg ctactgcggc ggcgttagat attttaggct ctattgacgc caatcgtggt 900

gatccgcagt taggctggga taccgaccaa ttcccgaaca gcgtggaaga aaataccctt 960

gcaatttatg aaatcttaaa agcaggcggt ttaaccaccg gcggcttaaa cttcgatgcg 1020

aaaattcgtc gtcaaagtat tgacccgtat gatttatttc atgctcatat cggagccatt 1080

gatgtgcttg cattgtcttt acgtcgagcg gcgaaaatgc tggaagatca aacgttacaa 1140

catatcgtgg agcaacgtta tgcgggttgg aacggtgaat taggccaaca aatcctgaac 1200

ggcaaatctt cgctggaagc tttggctcag gcggcccaac accttgaacc gcaaccggtt 1260

tcaggacaac aagaatatct tgaaaactta gtgaatagtt atatttatcg ttaa 1314

<210>65

<211>1599

<212>DNA

<213> Actinobacillus succinogenes

<400>65

atgaatgctg atgcaaaagt aaccgttcaa aacggccagg cttatttggg gattgaactg 60

ggttctaccc gaattaaatc cgtattaatc gacgaacaag ggaatattct cgccaagggt 120

agttttgaat gggaaaacca tttcaccgac ggtatttgga cgtatccgct ggatgaagtc 180

tgggcgggga tagcacaaag ttatcaggat atgagtaacg ccgttaaaag caagtatggt 240

attcggctca cccgccttgc cgccataggg atcagcgcta tgatgcacgg ttatttgcct 300

tttgacgcgc aggataacca gcttgcggaa ttccgcactt ggcgtaacaa caccacggcg 360

gtggctgcgg atgaactcac cgaacggttg caatacaaca ttcctcaacg atggagtgtc 420

gctcaccttt atcaagccgt gctgaatcag gaagaacatg ttaaaaatat cgcctatatc 480

actacattgg cgggctatgt tcactggcag ttaaccggcg aaaaagtgtt gggggtcggc 540

gatgcttccg gtatgttccc gattgattcg ggtattaaaa actacgatga aaatatggtg 600

aatattttca acgaactggt tgctgatcgg aattttaact ggacgctgaa tcaaattctg 660

ccgaaagtgc tagtagcggg tgagcctgcc ggggtattaa gcgaaaaagg cgcaaaatta 720

ctggatccgg ccggttatct gcaggcggga tgtccgcttt gtccgccgga aggcgatgcc 780

ggaaccggta tgatggcgac taacagtatt aaggtgaaaa ccggtaatgt ttccgccggt 840

acgtcggctt tcgccatggt agtgttagaa aaaccgttgt caaaagttta tcgtgacctg 900

gatatggtca ccacgcctga cggcgaaccg gtggcgatgg cgcattctca gaattgcagt 960

tcggatctga acgcctggtt ccattattc agtgaagttt tacgtacttt tcgcgccgat 1020

attacgaccg accatctata cgattcattg tttaccaaag ccttagaagc ttcaccggac 1080

ggcggaggct tgttgtctta ttgtttctat tccggcgaac acggagtgga tttgcttacc 1140

ggttgcccgt tattcctgca ttccgccaaa tccgaattta acttggccaa tttcattctg 1200

gtacaacttt atactgcctt cggtgcgatg aaactcggca tggatacgct gaccaaacag 1260

gaaaaagtta aggtagaaaa aatctttgcc cacggcggtt tgtttaaaag taaaggcgtg 1320

gcgcagcatg tactggcgtc ggcgttggac gtgccggttg cggtattgga taccgccggt 1380

gaaggcggtg cctggggaat tgccttatg gcggcataca cccgacaagc tgaccgtccg 1440

ttggccgatt ttttggaaca aacggttttt gccggcgccg tggataacgt tttggcaccg 1500

gatcccgttt gggtcggggg gtatgaaaaa tttattcagc gttataaaga cggattatcc 1560

gtcgaacggg aagcggctaa atttgcgaat cccgtgtaa 1599

<210>66

<211>1446

<212>DNA

<213> Actinobacillus succinogenes

<400>66

atgtatatcg gtatcgactt aggcacttcc ggcgtaaaag tggtgctgtt agatgaaagt 60

caacagattc tcgctactgt tcaacaagct cttcctattt cccgcccgca tccgctttgg 120

tccgaacaag acccgaaaga ttggtgggat gctgcaaatc ttgccatgct tgcgctttct 180

cgtgagcaaa atttatcggc ggtgaaagcc atcggtttaa ccgggcaaat gcacggcgct 240

accttattgg ataatcgaga taatgttttg cgtccggcga ttttgtggaa tgacggtcgt 300

agcgcagctg aatgtgcgga attagaaaaa ttagtaccga attcccgtga aatcaccggt 360

aatctcatga tgccgggttt taccgcacca aaacttaaat gggtggataa tcacgaaccc 420

gaggtagcag aaaaagtcag taaagtttta ttgccaaaag attatctacg cttaaaaatg 480

acaggtgaat acgcttccga tatgtctgac gcttcgggca ccatgtggct ggatgtggca 540

aaacgggat gggataaatc tttgcttaac gcttgcggtt tggatgaaaa cgcaatgccg 600

aaattatttg aaggcaatca aatcacaggt tattacgtg cggaactggc aaaaagctgg 660

aaaatgaata tcgtgccggt ggtggcgggg ggcggcgata atgccgcagg cgccatcggt 720

atcggcttgt ataaaagcgg gcaagcgatg ctttcactcg ggacgtcagg cgtgtatttt 780

gtggtgacgg ataaatttac cgctaacccg caaaaagcgg tacacagttt ctgtcatgcc 840

ttgccggatc gctggcattt gatgtcggtg attttaagcg cggcctctgc ggtggattgg 900

gtgcaaaaag tcacgggtat cgctaatatt caaactttgt ttgagcaagc ggaaaaaagt 960

gcggtggatt ccgagaccgt tttcctgcct tatctttcgg gcgaacgtac gccgcataat 1020

gacgcttatg ctaaaggcgt attctgggga ttaaaccata atgacaacca agccagtatg 1080

gcaaaagcgg tggtggaagg cgtcagtttc gctcttgccg acggtatcga cgtattgcat 1140

gaaacgggcg tgacggccga taatatcgcg ttaatcggcg gcggtgcgaa aagcgcttac 1200

tggcggcaac tgcttgcgga tatttcaggc agaaccatgg aatatcgcac cggcggcgac 1260

gtaggacctg cgcttggcgc ggcaaaattg gcgcaaatcg ctttgaatcc gaatgaaaat 1320

attgccgatt tgtgtcaacc gctgccgttg gaagccgtat atcggccgcg cgccgaacgc 1380

gctgcctatt acgcggaaaa acgggcgaaa tatgcacagt tatatcaacg cttaaaaggg 1440

ctttaa 1446

<210>67

<211>1605

<212>DNA

<213> Actinobacillus succinogenes

<400>67

atgcgcgatg tacaagcagt gattacctca ggcgaggcgt ctatcggaat tgaattcggt 60

tcgacgcgga taaaagcggt tcttatcaat gttcaaggcg atattttagc gaccggcggg 120

tttacttggg agaatcattt tgttgacggt atctggactt atccgcaaga tgaaatttgg 180

caaggtttac aagctgctta tcgcgctctc accgataatg taaaagaaaa atacggtctt 240

tccgtgacca aagccaaagc tatcggcatt agcggcatga tgcacggtta tatgcctttc 300

gatcaacggg gcggtttgct cgtgccgttc agaacatggc ggaataacat tacggcaaaa 360

tcttcgcaaa aactgaccgc actttttaac tataatattc ctcaacgctg gagtgtttcc 420

catttatatc aagcaatact taatcgggaa caacacgtcg gcgagattga tttctttacg 480

acgctggccg gttatgtaca ttggcggtta acggacgaaa aagtattggg cgtcggcgaa 540

gcatccggta tgtttccgat cgacccgaaa accggttctt attatcaaag catgctgaac 600

cggtttgatg aattggtcgc gccttatctct tatccctgga aaattacgga aattttaccg 660

aaggttttaa cggctggtca gtttgcggga aaattaaccg aacagggcgc aaaattgctc 720

gatccgacgg gacaattaca ggcgggtatt cctctttgtc cgccggaagg tgatgcggga 780

accggcatgg tggcgaccaa cagtattaaa gtcaatacgg gtaatatctc cgccggcact 840

tccgctttcg ccatgattgt attggaaaaa gagcttaccc gagtttacga acaactggat 900

tcggtgacaa cgcctgcggg caaattggtg gctatggcgc atgccaataa ctgtacttcc 960

gacattaatg cctggatcaa tttgttcggc gaaaatttaa aggtttttgg tttggaagtc 1020

gaaccggata agttatacga aaccttattc cttaaagcgt tagaaggcga ggcgaactgc 1080

ggtaatttat tagcttacgg cttttatcc ggcgagcaca gcgtgggttt atccgaaggt 1140

tgtccgactt ttatgcatcc ggctaattcg aattttactc tggcgaactt tatccgttct 1200

catttataca gtgcattcgg cgcaatgaaa ttaggcgtgg atattttaat tcaacgggaa 1260

aaagtcaaga tcgcccgaat tttagcgcac ggcggtattt tcaaaacgcc gaatgtagcg 1320

tctaaaatcc ttgcctcggc aattaatgtt cccgttgcgg taatgaagac tgctaatgaa 1380

ggcggagcat gggaattgc acttttagcg aattatttag cggcttatca agccggagaa 1440

tctttggaag actatcttga taaccggatt tttgtccggt cggaaattca aattacccgg 1500

cccgatgcgg aaatggcgaa aggttatgcg gaatttattc gaacttatca aaaaggtatc 1560

cctgtcgtcc gagcggcagt tgatgcgttc catttatgaaa tttaa 1605

<210>68

<211>1524

<212>DNA

<213> Actinobacillus succinogenes

<400>68

atgagtatag aaacaaaaga tgttgttatg cgtgctgaga atatctcaat gatattccca 60

ggtacaaaag cgcttgataa tgtgagttat aacgtttatc gcggcgctgt taatgtaatc 120

attggagaaa atggtgcagg taaatcaaca ttaatgaaaa ttcttgcagg tgtacagcaa 180

ccaactcttg gtgatttgta tatcaatggt gagaaagttc atattactga tacgcgtagt 240

gcagccgctc atggtattgg tatggtacac caggaattga atttatctga aaatctctct 300

gtcgcagaaa atattttctt aggtcgcgaa cttcaatcag gattattacc aattgacaat 360

aatgctcaat acgaagcatc aaaagaattg ctcaaacgtt taaaacaaga tattgatcca 420

aatgaaattg tttcaaattt aaaagttgga caaaaacaac tcattgaaat tgcaaaagca 480

ttagcaggca aaattgatat tttgatttta gatgaaccga cctctgcatt gagtaaaact 540

gaagtacaga ttttgtttga tgtgattcgc gaacttacag cgcaaggtgt ttccattatt 600

tatatttcgc accgtttgga agaattaatg gaaattggta cttatatcac tatattgcgt 660

gacggaaaat tccagtccga ggctgcagtg aaagatattg acgtgccttg gattgtgcgt 720

gaaatgttag gttctgatcc tgtgactgat tttttacctg aaaatcgtga atttggtgca 780

aaaattctgg aagcagaaaa tattacttgt atcaatgaat ctgaaaataa agtggtcaat 840

gatgtgtcct tttctttacg agctggtgaa atcgttggga tttacggttt aatgggagcg 900

ggacgtacag aattatttga atgtttatta ggacaagctg aatattcagg tcgtttttca 960

ttaaacggta tcatttattaa tgaaaatatt tcaactacta atcggataaa aatgggatg 1020

ggtttggtgc cggaagatcg taaacgttca ggtattttcc ctgttacttc agtaggtaat 1080

aacctcactg tttctagttt atggaaacgc ttgaaaaaac aatttgtaat ttctaaagaa 1140

gatgaggaga aagccatcca atcaaccatt aaagagcttt caattaaagt gtcttctcct 1200

gaggtagaga ttcaagcatt gagtggtggt aaccaacaaa aagtggttat tggtcgttca 1260

ttgttaactg aacctaaggt attgttactc gatgaaccaa cacgaggcat tgatattggt 1320

gctaaagccg atgtatttga gatgatggtt aaactttctc aacaaggtat tggtatttta 1380

ttctcaacat cagatctaaa agaaattatg gcagtttcag accgtatttt ggtgatgtct 1440

aatggtaaat taaccgcaga tgtgaaacgc tctgaaacta atgaagccgc tcttgttctt 1500

gcaagtgcaa aaggattcca ataa 1524

<210>69

<211>999

<212>DNA

<213> Actinobacillus succinogenes

<400>69

atgaaattaa aaacaacttt attaactctg gcggcagcga tcgccgtttg tgcaatccct 60

gctcaggcta aagatctcac gatcggtatg tccattgatg atttgcggtt agaaagatgg 120

caaaaagacc gcgatatttt tgttaaaaaa gcagaaagcc ttggcgctaa agtattggtt 180

caatccgcaa acggcgatga ttccgcgcaa atttcgcaaa ttgaaaacat gctgaataaa 240

aacgtagacg ttttagtcat tattccgcat aacggcgacg tattaagcaa tgtgatttcc 300

gaagcaaaaa aagaaggtgt gaaagtgtta gcttacgacc gcttaattaa taatgcggac 360

ttggattttt acgtttcttt cgacaatgaa aaagtaggcg aattgcaggc agatgcaatt 420

attaaggaaa aaccggaagg aaattatttc ttaatgggcg gttcgccggt ggataacaac 480

gcaaaattat tccgaaaagg tcagatgaaa gtgttacaac cgttaatcga cagcggcaaa 540

attaaagtgg tgggcgatca atgggttgac agctggctgg cggaaaaagc gttacagatt 600

atggaaaacg ccttaaccgc caataaaaac aacattgatg cggtagtcgc gtctaacgat 660

gccacggccg gcggcgctat tcaggcatta agcgcacaag gtttatcggg caaagtcgcc 720

atttccggtc aggatgcgga cttagcggcg attaaacgga ttgtagaagg tacacaaacc 780

atgacggtgt acaaaccgat caccaatctg gcggacaaag cggcggaact ttcggtggct 840

ttaggaaaag aggaaaaact ggaacctaac gcaaaattaa ataacggttt gaaagaagta 900

gatgcgtatt tattagatcc tatcgtcgtc accaaagata acatcgacag taccgtaatc 960

aaagacggat tccacagcaa agaggcagtt tataaataa 999

<210>70

<211>1128

<212>DNA

<213> Actinobacillus succinogenes

<400>70

atgtctaagt ttaaatctat aaatttacaa gtttatatta tgtttatcgc cattgtggtg 60

attatggcat tcttctttgt tactaccgac ggagcgtatt taagcgctcg taacatttct 120

aatttattgc gccaaacgtc catcaccggg attttagcta tcgggatgat tttcgtcatt 180

atctccgctg aaattgattt atccgtcggt tccatgatgg gattattagg cggtttcgcc 240

gcaatcgcag acgtgtggtg gggctggccg ttaccgttaa ccatagtcgt cacattagcg 300

gccggcgtgc tgttcggcac ctttacgggc tggtgggtgg cataccgtaa agtaccgtcg 360

ttcattgtta ccctggccgg catgttgatt tttcgcggaa tgctggtggg tgcaaccaac 420

ggtacaacgg tttcgccaat cagcaaagaa atgaccgcca tcgggcaagg ttatctttcc 480

gatacgacgg gaatgatttt aggtattgtt gccgtagtct gttttttcct gtggggcagt 540

tatcaacgca aagcccgcaa aaatcttaat cttaccgtgc cgaatgccgg taaagaaacc 600

atgaaatacg gcttattcac catttgtgtg ttaggggcga tttatctatt aaacgactac 660

cgcggcatcc cgtttcctgt attattactc gcgcttcttg ccgtaatcgg tatgtttat 720

gcgcgcaaaa ccgccttcgg tcgtcatatt tatgccatcg gcggcaatat tgaggcggca 780

agactatcgg gaattaatgt ggaaaaaatt aagttaatga ttttctccat gaacgggttt 840

atggtagcga ttgccggatt gattttaagt tctcgtttag gtgccggcgc accgtcagcc 900

ggtcaaaatg ccgaactgga tgctatcgcc gcctgcgtta tcggcggtgc cagtttagcc 960

ggcggtatcg gcagcgtata cggtgttgtt atcggcgcat taattatcgc tttgctcgat 1020

aacggcatga gcatgctgga tgtcccgact ttctggcaat atatcgttaa aggatcgatt 1080

ttattacttg ctgtatggat ggatactgtg agtaagaaaa aaatgtaa 1128

<210>71

<211>1515

<212>DNA

<213> Actinobacillus succinogenes

<400>71

atggctcaat tattagaaat gaaaaatatc actaaaaaat tcggtgatgt gactgcttta 60

aataatattt cgatttcgtt agaaaccggt gaaattctct ctttatgcgg tgagaacggt 120

tcgggtaaat ccaccttaat gaaagtatta tgcggcattt atccgtacgg tgatttcagc 180

ggagaaatta ctttttccgg cgaaccgtta atcgccaaag gtattaaaga tacggaagaa 240

aaaggcatt ccattattca tcaggaatta accctggtta aaaacatgac ggtgcttgaa 300

aatatgtttt tgggcaatga aatcgtaaaa tccggcatta ccgatgacaa tgaaatgtac 360

ctgcgttgca aaaccctgtt gcaacaagtg cagctggata ttgatccgaa taccaaagtg 420

ggcgaactgg gattgggtca gcagcaattg gtggaaatcg ccaaagccct caataaacaa 480

gtccgccttt taattcttga cgaacccacc gcctccttaa cggaaaaaga aaccgccgtt 540

ttattggcgt tagtcaaaga tttaagcgcc cataatattg cctgtgtata tatttcccat 600

aaattaaatg aagtaaaagc gatttccgac aaaatttgtg tgattcggga cggtgaacac 660

atcggcactc gtcctgccga gagcgtatcg gaagacgata ttatcaccat gatggtaggc 720

cgtgaaatca cgtcactcta tccttaccaa gaacaccaaa tcggtgaaga agtattgcgg 780

gtggcgaata tcaccgcctg gcatccgatt aatacccata ttaaacgggt cgacaatgca 840

aatttcaaat tacatgcggg tgaaatctta ggcgttgcag gacttgtagg ttccggacga 900

accgaaatgg cgcaatgcat cttcggttct taccaaggga aatctcaggg cgatattttt 960

atccgcaatc aaaaagtcaa aatccgtaat tgtgaggaag cgataaaaaa ccaaatcgtg 1020

atggtaccgg aagaccgtaa aaaacacggc attatcccaa ttatgggggt cggtaaaaat 1080

attacgctat cctcactcag acaattctgt ttcggcggaa aaatcattaa tgaaccgtta 1140

gaagaaacca taattaacca atccatcgag cgattaaaag tcaaaacgtc ctcctctgaa 1200

ttagctatcg gacgtttgag cggcggtaat cagcaaaaag ccattttagc caaatgcctg 1260

ctgctgaacc cccaaatttt gattttagac gaaccgaccc gcggtatcga cgtgggcgcg 1320

aaatatgaaa tctacaaact cattaatcgg ttggcgcagg aagggatggc ggtgattgtc 1380

atttcttccg aattaccgga agtattagga attagcgacc gggtattggt gatgcaccaa 1440

ggacagatta aagcggattt aatcaataat aatttaaccc aagaacaagt catggaagcg 1500

gcgcttaagg agtaa 1515

<210>72

<211>1119

<212>DNA

<213> Actinobacillus succinogenes

<400>72

atgagtcgag ttactgcgca ggatgtgaaa caaagcaatt acctcaacat ataccgtttg 60

ttttttcacc acgaattact gtcgaaaccc caaattgccc gtttattgaa actcagccta 120

cccactgtcg ttaataatgt atccgcttta gaagcggaag gaaaaattca agaaaacggc 180

atgtgtgcac cacaaggcgg cagaccggcc accgcttatc ggttggtgga cgatgccgcc 240

gtttccatcg gagtggaaat ccagtccaaa agtatcaaat gcgccgtcat taatctgaaa 300

ggcgatattt ctacagtgaa ttatttcatg gtgaattttg agaacagctt gaactatatt 360

caaagtctct gtcacactat aaacggattt attgcggatc agggctataa cgacagtcgg 420

attttagggg tggggatttc ctttcagggg atagccaata aagacggggca aactatttta 480

tacggcaaaa tcttacctta tgatcatctc agcgtcgcga cactgcaacc gtatttcgct 540

tatcccgtaa tcctgctgca cgatgtcaaa tgcgccgcca atgccgaatt atggcatgcg 600

aaacaacaca ttcataacgc cgtttatatt tccgtcagcg aacatctcgg cggcgcatta 660

attctcaata atcagattga tcaaggcaaa cagggctatt ccggcgcgct ggaacatttc 720

cgcataaacg acaacggtaa tccttgttat tgcggtcaaa tcggctgttt agaaacttat 780

tgttctttaa ccgcactttt acaatccggc gaatccctgg aacacttttt tctgaaactg 840

cgtaccgacg atcaaccgac ctatgcccgt tggctccgat tcttaaccta cttcgccaag 900

gcattgaatt acgtatatct tttgttggaa cgggacatta tttaggcgg agaaatcgcc 960

ccttatttac gcgatgaaga cctgtcgctc ttaagccggc tggtagaaaa tcatgccccg 1020

tttccgctga gcggacaatt catccgcatc gccgctttac agcaaaacgc agcgttaatc 1080

ggcgccgggt tgccgtttat ccgaaaatat atcccgtaa 1119

<210>73

<211>915

<212>DNA

<213> Actinobacillus succinogenes

<400>73

atgttatacg gattcgacat cggcggcact aagatagaac ttgccgtttt caatgaaaaa 60

ttagaaaaac agtacagcga acgcgtaccc accccgcaaa ataattatga cgagtggtta 120

tccgttattg tcagtctggt gcaaaaagcc gacgctaaat tcggttgtaa aggctcggta 180

ggcttaggct tgcccggttt tgtcaaccgg gaaacgggta ttgccgaaat taccaacata 240

cgtgttgccg acaataaacc gattttgaag gatttgtccg aacgcttaga acgtgaggtg 300

cgggcggaaa acgatgccaa ctgtttcgca ctttccgaag cctgggatga agaaaatcac 360

caatatccat tcgtgctagg attaattctc ggcaccggtt tcggcggcgg cctggtgttc 420

aacggcaaag tccattcggg taatatcggc atggcgggag aactgggcca tattcagttg 480

aattatcacg ctttaaaatt gctcggttgg gataaagcgc cgatatataa atgcggttgc 540

ggtaattctg cctgcttaga cacttatttg tccggtcgcg gcttcgaaat gctgtatcga 600

gatttacagg gcgaatcgct gcccgccaaa cagattatcg aaaaatttta cgctgatgat 660

aaaagtgcgg tcgaatttgt gaaacttttt gtggaactgg cggcgatttc catcggcaat 720

atcatcaccg cgttagatcc ccatttaatc gtgctgggcg gcggtctgtc caatttcgat 780

tatctttacg acgcgttacc gaaagcgctt ccttgccatt tgatgcgtag tgccaaagtc 840

cctgttatta aaaaagccaa atacggcgac tccggcggcg tacgcggtgc gacggcgctg 900

tttttaacac gctga 915

<210>74

<211>399

<212>DNA

<213> Actinobacillus succinogenes

<400>74

atgggttttt ttgctaaatt atttggctca aaagaaagtc aggcagtgga agtgaaagta 60

tatgcaccat tatcaggtga aatcgtgagc attgaagatg ttcctgatgt tgtattttca 120

gaaaaaattg tgggtgacgg tgttgcgatt aacccgaccg gtaatgaaat tgtcgcaccg 180

gtagacggcg ttatcggtaa aattttcgaa actaatcatg cattttctat ggaatctacc 240

gaaggtgtag aattattcgt acacttcggt atcgatactg ttgaactgaa gggcgaaggc 300

ttaccccgtg tagctcaaga aggtcaaacg gtcaaacgcg gtgatgtcgt attgaaattc 360

gatttggatt tattaaaatc aaaagcgaaa tcagtataa 399

<210>75

<211>1860

<212>DNA

<213> Actinobacillus succinogenes

<400>75

atggctaaag attttagcaa actcgcacaa gagattctcc gattcgtcgg cggagaacaa 60

aatattcatt cgctggtaca ctgcgccacc cgtttgcgct ttgtattgaa agaacgtgcc 120

aaagcggata aagcacggct ggaacaaacc gccggtgtta ttagtgtggt agaaagcggc 180

gggcagttcc aaattgtgat cggcaataat gtgccgaaag tatatgcgga gattatgaaa 240

atcgcagaca ttcaagcagg cgatgccgcc gatttgccta aaccgtcatt gataaatcga 300

ggaatcgatt ttcttgccgg ttcatttact cctcttatcg gggtgatggc gggagccggt 360

attttaaaag gcttaattgc aattttgcag gtttcgggct tattggatcc ggcttcaggc 420

acattcaccg tgctggacgc ggcagccagt tcaaccttct atttcttacc tattttgctc 480

gccattactt gtgcggccaa agtgaaagcc agcccttata ttgcggtcgc agttgccgca 540

gcgttagttt acccgagtat tgttgaggtg agctcaagcg gcaaaccgtt agacttttta 600

ggttttgaat tagctacgat gacttatacc tacagcgtaa ttccgattct gattgcggta 660

tgggctcaat cttatattca acgctttttc gaaaatcttt ggcacgaatc tgtccgcaat 720

ttctttgctc ccgcctgtac cttgttagtt gtagtaccgc tcactttagc cgttatcggg 780

cctgtcggcg gtgctatcgg acatggaatc gcaacggtat tggcttggct atatgccgcc 840

agcccgatag ccaccggtct tgttgtcgga gcattatggc aagtattggt gatcttcggc 900

atccattgga gcatcgtacc gattatgatc aacaatatgg cggttgtcgg tttcgatta 960

atcggcccgt taacggcagc tgcggtgtta ggacaagcgg gggcaacttt cgccgtcttc 1020

ctgaaatcac gcaataaaga gattaaatca cttgccggtt caaccacatt cagcggcgtg 1080

cttggttatta ccgaacccct tattacggc atcaccctac gctttaaaaa accctttgta 1140

tgcggtgtgc taggcggtgc tatcggcggt gcggttatcg gttggggcgg cactaaaatg 1200

tttgctttcg gcttctccgg tttattgctt taccctgtta ttatcggcga aggttcaaat 1260

attgtgatgt acaccgttgg aatggccgtt gcttttattg tagcaggcat actgacttat 1320

ctgttcggtt acagcgatac aatgctgccg caacaaactg cggatacggc aacaaaacct 1380

gaaaaaccga ataacgcccg ggtttctacg attccactcg aaaaatttaa tttagtcagc 1440

ccattagaag gcaatattca caaattaacg gatattcaag atccgatgtt cgcctctgaa 1500

gcgttaggaa aaggggtggc gattgaaccg accaaaggcg agttaatttc gccggtaaac 1560

ggcacgatca gctccgtatt tccaactaag cacgcctaca atattgtgtc tgacgacggt 1620

ttagaaatct taattcatat cggaatggat acggtacagc taaacggaaa aattttgaa 1680

actttcgtga aagacggcga tagaattcaa gtcggacaac ggctcggcac ctttgatatt 1740

aatgctatta aagcggcagg ttacagcata gtaacaccgg tgattattgc caacagcgaa 1800

gattaccttg acgttatcgc cagccaagag ccgcatattt atcaaaacag agaccgttga 1860

<210>76

<211>453

<212>DNA

<213> Actinobacillus succinogenes

<400>76

atgagtactg atttcattat tccgatgtcg gggaagttgg tatccctcga acaagtaccc 60

gatgaaactt tttgtcaaaa gaaacttggt gacggtttcg ccgtgatttt acagggcgaa 120

gtggttgtca gtccgatttc aggcgtggtc attgccgctt ttccgacagg acatgctttt 180

attatccgcc gggaggacgg tttggaagta ttgattcaca tcggactcaa ctcggcggcg 240

aaatccgaag cgtttcgtat gcagattgaa aaatatcagg aaatagaacg gggcgacgta 300

ttggtttata ttgaccgaca aaaactcggc gatacggagg ccgatctggt ttcgcccatt 360

gtgtttgcga atcccggaat cagaatcaat ctgttgaaat cggagcaaga cgtgttagtg 420

ggggatccgg acgccgttag cgtttcattg tga 453

<210>77

<211>1677

<212>DNA

<213> Actinobacillus succinogenes

<400>77

atgaatattt ttctgactca atctcaactc ggcaacgcca aagctttttt gttaaagcaa 60

gtgattacgg cggcggcaca agcccaaaat caccagatta cggataatgc cgaacaagcg 120

gatttggcta ttattttcgg taaaacattg ccgaatttga ccgcacttca aggtaaaaaa 180

gtctatttgg cggagccgga acaagcgtta accgcaccgg aacaaaccgt acaacgggct 240

ttaacggaag cgacggatta tgtcgcgccg cagcaatccg gcgtttcagc cgtcgcagcg 300

ggcggaagca aaaatattgt ggcgattacc gcctgtccga ccggcgtggc acacaccttt 360

ctgtccgccg aagccattga ggcttacggt aaaaaaatgg gctggaacgt tcgggtcgaa 420

acccgcgggc aagtaggcgt cggtaatccg attackccgg aagaagcggc ggcggcagat 480

ttggtattcg tagccgcgga tatcgatgtg gatttaagca agttcaaagg caaaccgatg 540

tatcgcacat cgaccggttt ggcgttgaag aaaaccgaac aggaatttac caaagccttt 600

gctgacgcta agattacga aggggggcgaa acgaaaacag ccgctgataa ttcggctcag 660

ggagagaaaa aaggcgttta tcaacatttg atgaccggcg tttcccatat gttgccgatg 720

gtggtagcag gcggtttgtt gattgccatt tcgtttatgt tcggaccgaa tgcctttaaa 780

gatccgagta ttgcgggcgg tttgccgacg gcgttaatgg aaatcggcgg cggagccgca 840

tttaaattga tgatcgccgt attcgccggt tatgtggctt attccattgc cgaccgtcct 900

gggctggctg tgggattgat cggcgggatg cttgccacca atgccaatgc cgggattttg 960

ggcggggattt tagcaggttt tcttgccggt tataccgtta aatttctgaa cgacatcatt 1020

aaattgcccg ccagtatgac atcgttgaag ccgattttga ttttaccgct gttaggctcc 1080

gctattgtgg ggctggccat ggtttacttc ctgaatccgc ctgtagcggc agtgatggcg 1140

tccttaaccg attggttaaa cggcttgggt tctactaacg gcttattatt cggagcgatt 1200

ctgggcggta tgatgtgcat cgatatgggt ggtccggtaa ataaagcggc gtacgtattc 1260

ggcaccggct tactggcttc tcaagtgaat acgccgatgg cggcaattat ggcgggcggt 1320

atggtgccgc cgttaggcat ggcgattgcc acttggattg cgcgtaataa attcaatgcg 1380

ggacaacgca acgcaggcaa cgcggcgttc gtgttaggct tatgttttat ttccgaaggc 1440

gcgttaccgt ttgtggcggc ggatccgatt cgcgtgattg tttccagtgt aatcggcggt 1500

gcgttagcag gggcgatttc tctaagcttc gccatcgaat taccggcgcc gcacggcggg 1560

ttgtttgtgg ttccgttagt gtcgcaaccg ttaatgtatc ttggcgcgat tgccgccggc 1620

gcattggtga ccggtgtgat ctacgccgtt atccggccaa aacaaacgca ggaataa 1677

<210>78

<211>1503

<212>DNA

<213> Actinobacillus succinogenes

<400>78

atgttcaatt tacctgaaaa taacattcat cttgccgctc aagctacgga taaacaacag 60

gccatcgaaa tggcggctgc cgcgttagag caggggggggt atgtagaaag cggttatta 120

caaggcatgc tggggcgtga acagcagact tccacctttt tgggtaacgg cattgccat 180

ccccacggta ccttggaaac ccgcgggtta gttaaaaata ccggtgtggc cgtgttccag 240

tttccgcaag gtatcgaatg gggagagggc aataaagctt atgtggtaat aggaatcgcc 300

gcccgttcgg atgaacattt gtcgttactg cgccaactga cgcatgtttt gggcgatgag 360

gacacggcgg caaaattggc cacattagat gatgtgaaga aattccgcgc aattctcatg 420

ggcgaagcgg aagactttgt cgtgaaagct gaaacgatca gcttagacgt agataccacc 480

agtttgctga ctttaaccgc gatcaatgcg ggtaaattac aggaacagag tgtggtggaa 540

aatgcctttg tttctgaggt tattagctca ccggcgttgc cgttaggaaa aggattatgg 600

ctgacggata gcgttgcggg caatgtcaaa aacggtttgg cattcagtcg ggcgcgcaaa 660

gcgttcgaat ataacggcaa aaccgtgcgg gcggtattaa cggtctcgtc caaagacgat 720

gcggtaaacg aaacgctggc acggttatg gatacgaatg tgcaggagca gctgttaacc 780

ggttcgcggg aacaaattgt tgcggcatta aacggtacgg aattggtagt gaatactgtg 840

ccggcacaag ccggcgggac tgtaatcgga accttcacgt tacgtaatga acacggtttg 900

catgctcgtc ccagcacggt tttagtgaat accttaaaag ccttcggcgc gaaaattaca 960

gtggaaaatc ttactcgcgg cggtttggcg gtgaatgcga aaagcgcaat gaagttagtg 1020

ggtatcggcg caactaaaaa tcatcgtttg cgttttgtgg cacaaggcga tgacgcagaa 1080

caggctatgg aagcgttggc aaaagccttt tccgaaggtc tgggtgaagg cgtgtccgcc 1140

gtgccgccgg cagaaccgga caccattgaa gtggtgtctg cggattcgac agccaatgtg 1200

ccggaaaaaa acgaggcaaa atccgccgac ggcgccgaag gcatttttgt actcaataac 1260

gagcacggtt tacacgcccg tccgagcgcg gtgttggtca atgaagtgaa aaaatataat 1320

gcttccgtgg cggtacagaa tcttgatcgt aatacccaat tagtcagtgc aaaaagcctg 1380

atgaaaattg tggcgttagg cgcaaccaaa ggtcatcgct tgcgttttgt ggcaaccggt 1440

gaagaagcgc aacaggcaat cgacggtatc ggtgcgatca ttgcggcggg gttaggagaa 1500

taa 1503

<210>79

<211>1050

<212>DNA

<213> Actinobacillus succinogenes

<400>79

atggctaaat taaataatgt aatgcaggag ttacaaaaag cttttaatac gggcgtatct 60

tttatgttac cggcagtggt tgtcggcggt attttcttag cattagcatt atcgacaggc 120

gatgcgacag atgcgggtat gagcgtcact aatccatta tgaaaaatct gagtgatctt 180

ggtgttgccg gtttttccgt catgattcct attttagcag gttatatcgc aaattctatt 240

tcaggcaaac ccgcactggc accggcaatg attttaggtt atgtcgcaaa taatccggtt 300

ggagaaggcg gcattaaaac agggtttctt ggcgctatta tcatgggaat tgcagtcggc 360

tattttgtga aatggtgtaa aacatggaaa gtgccaaata cgattaaaac tattatgccg 420

atcttgattg ttccggtgat tacgacgttt gtactaggta tggcgtatat ctatattgtc 480

gccgaaccta tcggatttgc aatgaaacag ttagttgaaa ttttaggtga attgcaagga 540

ggtagcgcga ttgttctcgg attaattatt ggagccatga cggctattga tatgggagga 600

ccgattaata aaacggcgac agcttttact ctcgcactaa tggcagaagg tattttcgag 660

cctaacggcg cacatcgtgt tgccgttgct attccgcctt tagctatggc gttatctact 720

ttcatagatc gcaaaaaata tacgtcggaa gataaagatc tcggtatttc ggctttcttt 780

atggggctta tcggtattac tgaaggggct attccatttg ccgttaaaga tatgaaatgc 840

gtattacccg caattattat cggtagtgcg gttggcggtg cgttatccat gataaatggc 900

gtagaaacgc ttatccctca cggcggttta attgttcttc cggtagttaa cggcaaactt 960

tggtttttct tgtcgatgct tatcggcacc ttagtttcag ttgttattct ccactttacc 1020

aaaccaaatt tagaatcaga taaagct taa 1050

<210>80

<211>318

<212>DNA

<213> Actinobacillus succinogenes

<400>80

atgaaaattg tcgcaattac agcttgccct acaggcgtgg ctcatactta tatggctcaa 60

gaagctattg aaaaagaatg taaaaaaaga ggtcatcaaa ttagcgttga aacccaaggc 120

agtatgggag ttgagaatga attggaccaa tccgaagtgg acgctgcgga tgttgtgatt 180

cttgccgttg caattgccat tgaaggtgaa gagcgttttg aagaaaaacg tgaccttggg 240

aaagtaattg atatcgagcc gggtaatgcg atcaaacaca ttgttgattt gattgataaa 300

gctgaggctt tagcataa 318

<210>81

<211>447

<212>DNA

<213> Actinobacillus succinogenes

<400>81

atggaaaaat caattatttc agagcagctc atcattctcg atttagatgc ggataacaaa 60

aataccatca ttgaagcgat ttcccagctc gcttttgagc atggttatat ctcggattta 120

ggacaattta ttgaaacagt gaagcaacga gaagaacaat tttcgacagc gattggttat 180

ggcatcggaa tgccacatgg caaatcaaat actgttttat ctccctttat cagttttgca 240

cgattaaaaa taccatttta ttgggatgaa gaagagctgg tgaaatatgt ttttctgatc 300

ggagtgcctg aagcagacgt ggtacttcat ctcaaattta tttcgcagct aagtaaaaaa 360

ttgttggatg acgattatcg caatgcactg gctgattttg agacgacgaa acaagtcgaa 420

gcccatttga gccaaattga aatttga 447

<210>82

<211>942

<212>DNA

<213> Actinobacillus succinogenes

<400>82

atggcgaaag tagcaactat taccttaaat gccgcttatg atttagtcgg tcgtttgaaa 60

cgtatcgaat taggcgaagt taacacggtg gaaacgctgg gtttatccc tgccggtaaa 120

ggcattaatg tggcgaaagt gctgaatgat ctcggtgtca gcgttgccgt cggcggtttt 180

ctgggtaaag acaacgtcgg tgatttcgag cgcttgtttg cttccgtcgg tttggaggat 240

aaatttcagc gggtagccgg taaaacccgt attaatgtaa aaattaccga aacggaagcg 300

gatgtgacgg atttaaactt cttgggctat cacatttcgg cagcggattg gcagaaattt 360

gtagcggatt cgttagctta ttgccaagcc ttcgacatcg tggcggtttg cggcagcctt 420

ccgcgcggtg ttacgccgga attattcgcc gattggttac gtcagttaca tcaagccggc 480

gtaaaagtcg tactggacag ttccaacgct gccttaaccg cggggttaac cgcacatcct 540

tggttagtga aaccgaatca tcgcgaatta gaagcttgga ttggacacag cttgaacagt 600

ttggaagaaa ttgttcaggc tgcaaaaaaa ttgcagacgc aaggcattgc caacgtaatt 660

attccatgg gagccgaggg ttcgctgtgg ttgagcgaac agggcgtagt acgggcgcaa 720

ccgccgaaat gtgaaaatgt ggtaagtacc gtcggtgcag gcgattccat ggtggcaggg 780

ttgattacg gtgtagtaaa cggattgtcc caacaggata ccttggcgtt tgccagtgcg 840

gtttcagcct ttgccgtatc gcaaagcaat gtgggagtca gtgatcgggc gttactcgaa 900

ccgattctcg ccaacgttaa aattacaacg attgaaggat aa 942

<210>83

<211>942

<212>DNA

<213> Actinobacillus succinogenes

<400>83

atggcgaaag tagcaactat taccttaaat gccgcttatg atttagtcgg tcgtttgaaa 60

cgtatcgaat taggcgaagt taacacggtg gaaacgctgg gtttatccc tgccggtaaa 120

ggcattaatg tggcgaaagt gctgaatgat ctcggtgtca gcgttgccgt cggcggtttt 180

ctgggtaaag acaacgtcgg tgatttcgag cgcttgtttg cttccgtcgg tttggaggat 240

aaatttcagc gggtagccgg taaaacccgt attaatgtaa aaattaccga aacggaagcg 300

gatgtgacgg atttaaactt cttgggctat cacatttcgg cagcggattg gcagaaattt 360

gtagcggatt cgttagctta ttgccaagcc ttcgacatcg tggcggtttg cggcagcctt 420

ccgcgcggtg ttacgccgga attattcgcc gattggttac gtcagttaca tcaagccggc 480

gtaaaagtcg tactggacag ttccaacgct gccttaaccg cggggttaac cgcacatcct 540

tggttagtga aaccgaatca tcgcgaatta gaagcttgga ttggacacag cttgaacagt 600

ttggaagaaa ttgttcaggc tgcaaaaaaa ttgcagacgc aaggcattgc caacgtaatt 660

attccatgg gagccgaggg ttcgctgtgg ttgagcgaac agggcgtagt acgggcgcaa 720

ccgccgaaat gtgaaaatgt ggtaagtacc gtcggtgcag gcgattccat ggtggcaggg 780

ttgattacg gtgtagtaaa cggattgtcc caacaggata ccttggcgtt tgccagtgcg 840

gtttcagcct ttgccgtatc gcaaagcaat gtgggagtca gtgatcgggc gttactcgaa 900

ccgattctcg ccaacgttaa aattacaacg attgaaggat aa 942

<210>84

<211>930

<212>DNA

<213> Actinobacillus succinogenes

<400>84

atggctaaca aaatctgggt tcttggcgac gcggtggtgg atttaatccc tgacggtgag 60

cgacattatt taaaatgcgc gggcggtgcg cccgccaatg tcgccgtagg tgtggctcgc 120

ttaggcgtgc cgagtgcatt tatcggccga gtggggaaag atccccttgg tgaattcatg 180

caacaaacgc tgaaggcaga aaacgtaaat acggatttta tgtacctcga tcccgaccat 240

cgcacctcaa cggtcgtggt cggcttggat aacggcgaac gcagttttac ctttatggtg 300

aatccgagtg cggatcagtt tttaacggat tccgatttgc cgccgtttca agccggtgaa 360

tggctgcatt gctgttctat cgcgttgatt aacgaaccga cccgcacggc aacttttgcc 420

gcgatgaaaa atatcaaagc ggcgggcgga aaagtttcct tcgatccgaa tctgcgcgaa 480

agcttgtggc aatcgcaggc ggaaatgatc gatgtggtca tgcaagccgt cgcgctcgcc 540

gatgtgctga aattctccga agaagaatta acgctgctca cccgcaccga cagccttgaa 600

aaatctttcg aaaaactgac cgcactttat ccggaaaaac tgatcatcgt gacaatgggt 660

aaagacggtg ccttgttcca tttaaacggt cagcgtgaaa ttgtgagcgg aaaagcccta 720

aaaccgatgg ataccaccgg tgccggcgac gcctttgtgg gcggcctgct ggcaggactg 780

gcattacgtc cggactggca aacaaatacg gaaaaactta aaaccatcat ccgccaggcc 840

aacgcctgcg gcgcattagc ctgtacggcg aaaggcgcca tgtccgcctt gccgaatcag 900

cggcaactgg ccgaattttt gacggattaa 930

<210>85

<211>1728

<212>DNA

<213> Actinobacillus succinogenes

<400>85

atgatttcag gaattccagc ttctccaggt atcgttttcg gtaaagcact tgtacttaaa 60

gaagaaaaaa ttgttatcga tcatagcaaa atcggcgacg atcaactgga tgccgaagta 120

gcgcgtttct atgaggggcg cgctgcggca gtggaacagt taagttctat ccgcgatcgt 180

gcattgaaat cgctcggtga agataaagcg gcgatttttg aaggtcattt aatgatcttg 240

gaagatgaag aattggaaga agaaatcatt gattaccttc gttcgcacaa agttaatgcg 300

gcggcggcgt tgagtaaagg tatcgatcag caagtcgcta tgttgtcgga aattgatgac 360

gaatattaa aagagcgtgc gggtgatatt cgggatatcg gtaatcgact gcttaaaaat 420

attttaggaa tgtatattgt ggatttgggc gaaattaatg aagaagctat tttggtagct 480

tacgattaa ccccctccga aacggcacaa ttaaatttag ataaagtatt agggtttatt 540

accgatatcg gcggtcgcac ctctcatacg tcgattatgg cgcgctcctt ggagttaccg 600

gcgattgtag gtaccaataa tgtaacggaa aaagtcaaca ccggcgattt cctgattctg 660

gatgcggtaa acaatgcggt ttatgtgaat ccgtctcaag aacaaatcga tagcttaaaa 720

gcgcagcagg cacagcttgc ggcggaaaaa gcggaattgg caaaattaaa agattgccg 780

gcattaacat tggatggtca tcaggttgat gtggttgcaa acatcggaac gattcgtgat 840

tgcgaagggg cggatcgtaa cggtgcggaa ggtgtcggtt tataccgtac cgaattcctg 900

tttatggatc gcgacagcct gccttcggaa gaagagcagt ttaccgctta taaagaagtg 960

gtggaagcca tgaacggtcg tttggtggta ttacgcacga tggatatcgg cggcgataaa 1020

gaattaccgt atttgaatct accgaaagag atgaatccgt tcttaggttg gcgtgccgtg 1080

cgtattgcct tggatcgccg tgagattttg catgcgcaat tacgtgccgt actgcgtgct 1140

tcagcattcg gtaaattcgc ggtgatgttc ccgatgatta tttctgttga ggaaattcgt 1200

gagttaaaag ccgtgatcga agtgctgaaa caagagcttc gcaatgaggg taaagcattc 1260

gatgaaagta tccaaatcgg tgtaatggtt gaaacgccat ctgcggcggt gaatgctaaa 1320

ttcttagcta aagaagtgga tttcttcagt atcggtacaa acgatttaac ccaatatacc 1380

ttagcggtag accggggtaa tgaattaatt tctcatttat ataaccctat gtctccgtca 1440

gtattaagcc tgatcaaaca ggtaatcgac gcttctcatg ccgaaggtaa atggaccggt 1500

atgtgcggtg aattagccgg tgacgaacgt gcgactttat tgttgttagg tatgggcttg 1560

gatgaattca gtatgagtgc gatttccgtt ccccggatta aaaaattaat ccgtaatgta 1620

aactttgccg atgcgcaaat tttagctgaa aaagcgttac aggttccgac tgcggctgaa 1680

attgaaaaat tagtcgatga tttcttgaaa gaaaaagcat taaactag 1728

<210>86

<211>258

<212>DNA

<213> Actinobacillus succinogenes

<400>86

atgtattcaa aagacgttga aattacagct gccgccggtt tacacacccg tcccgccgca 60

caattcgtaa aggaagcaaa agcattcgca tctgacatta ctgtcacatc agcaggtaaa 120

agtgcaagcg caaaaagctt atttaaatta caaactttag gtctaacgca aggtaccgtc 180

attacaattt cggctgaagg tgaagacgaa caaactgccg ttgatcattt agttgcttta 240

atgccgactt tagaataa 258

<210>87

<211>1356

<212>DNA

<213> Actinobacillus succinogenes

<400>87

atgaaccgac aaaataaatg gttaaaacgt atcggttacg gtttcggcga tttcggctgt 60

aacttagtat tcagtaccat ggcgtcttac cttatgtttt tctataccga cgtattcggc 120

attgaagccg ccgtggtcgg taccatgatg ttatccaccc ggttgttgga tgccgtgacg 180

gatgtactga tgggattggt tgttgaccgt acgcataccc gctggggttc cggtcggcct 240

tattttgtga tcggcgccat tccgtttgcg atttttacca cattaacgtt ttacgtgcct 300

gatttcggta cggcggggaa aatcatttgg gcctattgca cttatattat gctttctctt 360

gcctatactg tagtgaatat tccgttaaac accattgttc cgcgtttaac gtcggacatt 420

aacgaacgaa atattctggt ggcaagccgt atgatttgcg ccttgttggg gacgaccgtg 480

gtgatgggga ttacccaacc gttggtggat ttcttcggcg gcggcgacta taagcaaggc 540

tatttcatca ccatgacggt ttacggcgtg ctggccatgt tgattttctt ttttactttt 600

acccaaacgg aagaagtggt accgccaacc gtcgtgcgaa gcgaaaaatc ttccgtatgg 660

gatgatttca gaggactgac ttcacaatcc tggattttag ttttagtgaa tttcttctat 720

ttcggcctgt tcgttgtgcg taatacttcc gtaatttatt actttacata taacctgaac 780

agtaccggat ggttgacctt cgtcggcttt ttcggggattt tatccggttt accgattctg 840

ttgttattgc ctcggctgca aaaaatcttt ccgcaacgca ccctgatcat cgcctgctgt 900

gcgctttatc ttgtcggtga tgccattgcc tacatcggta aggattctct gttcctgcaa 960

ctgaccagtt tagccgttac cggtttggga atgtacggta ttttcggtgt gacttttgcc 1020

attcaaccgg atgtgattga ttattccgaa tatgagaaaa accgcagtat accgggtatg 1080

atcgcctcaa tgcagggatt tttcgtaaaa ttcggtatgg gcgtagccgg actttctatc 1140

ggttggatcc ttgaggccgg cggttatcag ccgaatgtgg cgcaaaccga ctccgcttta 1200

ttcagtatcg aggtttgcta catttggatt ccggtcatta tttgtctcag tattgtggca 1260

ttaatgagct tctacaaact ggacggatta cgcagcgaaa tgagccgggt attggattta 1320

cgcagaaaac aaatggaata cgccgataaa ttttag 1356

<210>88

<211>1449

<212> DNA

<213> Actinobacillus succinogenes

<400>88

atgataattt tcaataacgg caaatacaaa tccttacatg cggcgcaagc cggtgaactg 60

gaaagcataa aaaatacggt ggaaaacgac cggaattttc gccctgttta tcaccttgcc 120

gccccgaccg gcttattaaa cgatcccaac ggtttaatct gggacggcga aaaatatcat 180

gtattttacc aatggtttcc tttcggtgcc ttacacggca tgaagcattg gaagcattac 240

accacgacgg attttatca ttaccgccaa gcacaggatc ttatcccga cgaattgttc 300

gaaagccacg gttgttattc cggcggcgca ctgttggccg gtgataaaat cgcctgtttt 360

tataccggca acacgcgtcg cccaagcgat aatcaacgtg tgccgtttca gaatttagcc 420

cttttcgaca aagcgggaaa tttactcggg aaacgtcctt tactgaaaaa ttcgcccgca 480

ggttacaccg aacattttcg cgatccgaaa ccgttttttg catctgatgg aaaaattcgc 540

tttatttgcg gcgcacaacg ggaaaatatg agcggcaccg ccctgctgtt tgaaatggag 600

aatcttgagg acaagccgca attattgggt gaactgcaac tgcccgcctt caataatcaa 660

aatgtattta tgtgggaatg tcccgatta ttgaaaatcg acaatcgcga tctgtttat 720

tggtcgccgc aaggcaaaat ccgcgaatcc tatcggtttc agaataatta ccatgctgcc 780

tatgctttgg ggcgactgga cggtttaact ttccaagccg aacggattgc ggaattggat 840

caaggttttg acttttatgc gccgcaaacc tttgccaata cggcggctcg tacaatctta 900

ctggcgtgga tcggcttgcc ggatttaact tatccgacgg ataaatacaa atggcattcc 960

atgattactt tgccgcggga acttcgggta gaaaataacc gcttatatca acaaccggta 1020

cgggaaattt atcaaaatct gaccgcactt gaatctgtca gcctgacggg caaagcggaa 1080

attcacaatc tggatcgcgc atatctgaaa tttaatgcgg aaaatcagcc gtttcgtctg 1140

aatttcttca ctaacgctaa acagcaaaac ctcactctgt cttacgacgg caacctgctt 1200

tgtcttgacc gttcgcaaac ggagcagacg gatgaaatga aaaaattcgg cgcacaacgt 1260

tattgcgaaa tcgaaaatct tcagcaagtc gagatttttt tcgaccgttc cgtatgcgaa 1320

atatttctga atcggggcga aaagacgatg acttcgcgct tctttatgga aaatcggcaa 1380

aacgtcgtcg aaatcgaccg cactttgcac atcgcaatcg gttacccacg cataattgac 1440

gtgaaataa 1449

<210>89

<211>1386

<212>DNA

<213> Actinobacillus succinogenes

<400>89

ttgcaaacga caaaatttta ttcgctattt tcaggtattt tctttctgtt tttcggttac 60

gggttatttc ttaactctgc aggggttaaa ttggcggaaa tgggggtaaa cgatgttatc 120

atcggtttcc tgaatgcggc tttctttgtt ggagctgcac tcagcgccgt atttgcccat 180

cgtattgtat cgagtgtcgg gcatatccgt tctttcagtg ttttcggtgc gatttttgct 240

attgccgcgt taagccacat gatggtggat gatttgtggg tatggggggt attgcgtatc 300

gttctcggtt tttgtcatta tagtttacta ttattaggtgg aaagctggat gagcgaaaaa 360

accaacgtag agactcgcgg tcgggtactg gcaacctaca atattatttt ttaccttgcc 420

ttcattctcg gggttagttt gcttagtttg gaattaacca gtaataacat ttttacgttg 480

gcgactattt tggtcgtaat gtcgatgatt cctattgcgc tcactaaaat gaaacagccc 540

gagattcctg aacgcgaacg tatcagtatt cctcattttc ttgcggtatc gcctttggca 600

ttggctacca gttttatcag cggtatgttg gtaaatggtt tttttactat ggtgtcggtg 660

tttatgttga aattggattt ttcgcttggt gaaatttccg tttatttaat gatttccatg 720

gtcggcgggt tcgtcagcca attgccggta gccggtctat cggatcgatt cggccgacgt 780

aataccattt tattttgttc tttaattgct tccgttaccg cagtcatcgg gattgtcgct 840

gtttttagac agcattcgcc atactggttg caatatgtgg ttgccttttt attcgggtgc 900

agtttgttca cgttgtatgc gttaagtatt gcccgtgcca atgacgaatt accgaataat 960

atgagtacgg tagaagtcag ccgcagcctg ttattctgtt acggtatagg cgcgttaacc 1020

gctccgccgt tgctcgggat ggcgattggg gtttcggaac aatacggttt ttatctttc 1080

ttcggattgt tttcgttgat tttgctcggt tttgctatag ccaccaagcc gattccgcgt 1140

gcccaccgcg tagaaatgca gttatctacg cctatggcga cggccactac ggtggatgaa 1200

atagaaacca gcgaagaaac gctcgtaccg tttgatgaag aattggttca ggaataccaa 1260

gaacatttgg aaacgctgga tgctgagtca acagaggaag aaaaagaacc ggcagcggga 1320

gaagagaaag aaaaagaatc catgaaagaa gaaaatgaat cgatagagga agatgactta 1380

aaataa 1386

<210>90

<211>831

<212>DNA

<213> Actinobacillus succinogenes

<400>90

ttcttcgtcc atccgcagtc tttcgccttt gcccgcgaat atgccgaata cagccggcgg 60

gaagcggtga tgtttaccac catttatgcgg accccagattt cattggcgtg gattatcggt 120

ccgccggttt cctttgctat cgcattaaac tggggattta atttatgta tctggtggcg 180

ggcttggcat ttttattttg tgcgctgtta accgctacta tgctgcccaa aatcagccgc 240

aaacaaatgc tgaaaaccga atccgcgccg ttaagcgcac caagcgacaa ccgtaaatcc 300

gtgtcttatc tgttcatcgc ctgttttttg atgtggatgt gcaacagtat gtatttgatt 360

tccatgccgt tgtacaccat tcatgagtta catttgtcgg aaaatttggc aggaacctta 420

atgggaacag ctgccggtct ggaaattccc gttatgttga tcggcggtta tctcaccaaa 480

ttttttcgaa aaaaaacctt aattttgacc gcacttttgg ccggttttac cttcaatctg 540

ctgttacttt ttctgcatga aacttggcag cttgttacat tacaagcgct aaatgcggtt 600

tttatcggta ttatcgccac tctcggcatg gtttattttc aggatctcat gccgcgtcaa 660

atgggggccg ccactacact attcggtaac gcggcaaaag gcagttggat tctaggcggt 720

ccaatcgccg ggttaatcgc ggaacactgg ggctacagct cggtatttta tttatccgtg 780

attattgtgc tgatcagctt ttactgtatg tggaaggtga agtcagttta a 831

<210>91

<211>1296

<212>DNA

<213> Actinobacillus succinogenes

<400>91

atgtcattca tcgtattgct tggagtaatg tttatcctta cggtgctcgg tttaccatg 60

ttttatagta ttatcttcgc atctattgtg attattca ctttcttacc taatatcccc 120

gtgacaatcg tagcacagca agtaatgcaa ggacttgata ctaacgcttt gcaggcgctc 180

gcatttttct ttttagccgg agaattgatg agtgtcggcg ggatcacttc acgtattatc 240

cgtttcgtca cctcattaat cggtcgctgg aaaggttcgc tggcctatat caatatcttt 300

tccagtttat tttttggttc tatcagcgga tccgcagttg cctctacggc ggctattggc 360

agttctttga ttcccgcaat gaagaagacc ggttatccgg cggatttctc tgccgcactg 420

acacaatccg cttccattat cggaccgatc attccgccaa gcgtacctat gattgttttc 480

gcggctttag ccggcgcggg tgtatccgta gggaaaatgt tcatgtccgg cattattccg 540

ggtatcatga ttgcgcttgt gctgatcctg gttactttta tattaagtaa aatttataaa 600

tacggtaatc aggcaaccgc attcagctgg aaagaagtga aagatagcgg aaaagatgcc 660

gtatgggcaa tattttgtcc ggttatattatt ttatacggta ttatttcggg cgttttcact 720

gcaacggaag cgggggcggt ttctgttgtt tatgcctatc tggtcggtac attcgtatat 780

aaagaattaa gtattgaact attgaaaaaa gcgttgattt cttcactcaa aggaaccatt 840

acggttatgc tgattgtcgg agcatcttca atctttgctt ggttaattgc tcgtttacaa 900

atcagccatc aggtagccca ttgggtgtct tctgtttgcg atagccctct tcaggctctg 960

ctattgatta atgttattgt gttgttcatc ggaatgatta tggatccgac ggcggcattg 1020

acaattttag taccggtatt tatgccgatc gttaatcagt ttggtatcgc accgattcac 1080

ttcggtttgg taatcatcct gaatctgatg ataggcttgg ttacaccgcc ggtgggctat 1140

ttgattttcc tctcggccaa cattgcggaa tgtgaaccga tgaaagtgct taaatcaagt 1200

ttaccgttct tgttcacatt attcgcatta ttgttatttc tcatttttgt acctgaattc 1260

agtacatatt tacctgattt attgtttcca caataa 1296

<210>92

<211>1293

<212>DNA

<213> Actinobacillus succinogenes

<400>92

atggacgtta ttaattcctat ttctattcta ttgtttctct ttgttattgg aacaccggtc 60

gctttctgta ttttttgttc aacattaatc tattttttaa tgagtaattt acctatggta 120

atgttgattc aacgactagc tggcggtttg gaaagcgtta ctttacttgc gattcctttc 180

ttcatttatgg ctggagtatt tatgaatcac acagggatta gtgaacgatt actcaagttt 240

tgtgaagtat taacggggca tatgagtggc ggtttggctc aggttaatgt cgcattgagc 300

acattaatgg gcggtttatc cggttcgaat attgcggatg ccgcaatgaa ctcgaaatta 360

cttgtacctc aaatggttgc gcgtggttat agtgcttcat tttctgcagc cgtaacagct 420

gccggatcgc tcattacccc tattattccg cccggtatag cgatgattat ttatggttat 480

gtcaataatg tttctattgg gcgtttatt ttagcgggta ttgtccccgg aaccctgctt 540

tgtatcatga tgatggtttt ggtttccatt atttctaaaa agcgaggtta tttgcctatt 600

cgagaaaagc gtgcaagctg taaagaaatc attgtttcgg ctaaagatgc ggtattggct 660

cttttactgc caattatcat tattggcggt attcgaatgg ggatttttac acctactgag 720

gctggggctg ttgcggtgat ttatgcatta atactaggca tgtttatta tcgcaatatg 780

gatttgaaaa aactttggct ggcaacccga gaatcggcat taggcgcggc gaacgtattg 840

ttaataatct gtgtcgccgt agcattctca aaattcttga cttgggagcg tgtgccgcaa 900

actcttgcga gctggatgac aacagtggtt gatagtccga ttacgttctt aatgctggtt 960

aatgttgctt tattgatttt aggtatgttt ttagaaggta atgcgatcat gatcgtattg 1020

gcgccgttac ttgcgccaat tgcacattca tttggcattg atcctattca tttcggtatt 1080

gtttttattt tcaacggggc aattgggacg attacgcctc cattaggaac cgtaatgttt 1140

acaacgtgtt ctattaccga agttcctatt gagaaattta tcaaagatgt cttaccgttt 1200

tggggactcc ttatattgga actcattcta ttaacttata ttccgacaat caccacttgg 1260

ctgcctaatt tagtttatgg cgtagcacaa taa 1293

<210>93

<211>1278

<212>DNA

<213> Actinobacillus succinogenes

<400>93

atgaccgtag taatttttct ttctgtttta ttaagcgcga tcattctggg cattcccgtt 60

gctttctctt tactcgtctg cggtgtggcg ttaatgctgc atttggactt gttcgattct 120

cagatcttgg cccaacaaat tgtaagcggg gcggatagtt tttctttaat ggcgattccg 180

ttctttatc ttgccggtga gctcatgaat gaaggcggct tgtccaaacg tattatcgat 240

ttaccgatga aacttgtggg gcataaacga ggcggattag gctttgtcgc tattcttgcg 300

gcaatgatta tggcgagtct ttccggttcc gccgtagcgg ataccgcagc ggtcgccgca 360

atgttgttac cgatgatgaa aaccaccggt tatccggaag cccgttctgc cggcttaatc 420

ggtaccgccg gtattattgc accgattata ccgccgtcta tcccgtttat cgtatttggg 480

gtagccagcg gcgtgtctat cactaaatta ttcttagccg gtatttttcc ggggattttc 540

atgggattat gtttaggtct gctttggtgg tggcaggcaa aacggttaaa cctgatgacc 600

ttttccaaag ccaccagaca ggacctttgc atttccttta aaaacagtat ttgggcatta 660

ttgcttccgg tgattattat cggcggtttc cgtaccggta tttttacccc gacagaagcc 720

ggggcagtcg ctacttttta cgcgttaatc gtctcattgt tcgtttaccg cgaattgaaa 780

ttcaaagatt tgtacaaagt cgttttagcc gcttccaaaa ccacggcggt cgtcatgttt 840

ttagtagcgg cggcaaatgt cacgggttgg ttaattaccg ttgcagaatt gccgatgatg 900

ctaacggagt tactggaacc gttgattgaa acgccgacaa ccttattgct tgttgtaatg 960

ttagcggtat tcgtcgtcgg tatggtgatg gatttaaccc cgacggtgct aatcttaacg 1020

ccggtattaa tgccgttaat tcaggaagcg ggaattgatc cggtgtattt cggcgtactg 1080

ttcatcctga acacctccat cggtttgatt accccgccgg tgggtaatgt cttaaatgtg 1140

attaccggcg tatcaaaatt accgtttgat aaagcggcaa gaggcattat gccgtatctg 1200

atcatgatga tcatgctgct ttattattc gtctttttcc catcattagt gttagttccg 1260

ctaagttgga tgcagtaa 1278

<210>94

<211>1272

<212>DNA

<213> Actinobacillus succinogenes

<400>94

atgacaattt tatttattac cggcttattt attacattaa tcatttatcgg tattcctatt 60

gcattttcga ttgcgattgc cgcgcttatc ggtattcata tgattccggg cgtaccaccg 120

accatgatac cgatgaagat gttttacggt ttaaattcct atgtgttgct ggcggtacct 180

ttgtttgttc tcaccgccag tattatgaat caaggtaata tttcacgtaa actgattaac 240

ttttctttag gaatagtcgg gcgttgtccg ggcggattag ggcatgcaaa cgttatggta 300

tcgatgttgt tcgccggcgt aagcggttcg tctcaagccg ataccgccgg catggggaaa 360

attttaattc cccagatgat cgaacgcggt tacagtaagg aaatggcggt gggcgtcacg 420

gcggcgtcct cgacaatcgg tagtattatt ccgcctagta ttactatggt aatttacggc 480

ggtctgacca atacgtccgt aggtaaattg tttataggcg gtattattcc gggacttact 540

atcggtatcg gtatgatgct ctttattattac tatgtggccg ttaaacaaca tttaccgaaa 600

gaagaaaaat taccgttgaa attgttctta cgattatta aagattctct gcccgcattg 660

ttcacaccgt taatcattat cggcggtatc gttttcggta ttttcacacc gacggaagcg 720

gcggcgtttt ccgccatta tgcttatttt gtcagcgttt attackacaa aacgatcacc 780

ttcggagatt taccggttat tctggtcgat accttaaaat taagctgttt atcgctgttt 840

gcattagcgg ccgcttcggc attaggcgaa tttttaggtt atttcaaaat caatgcgatg 900

gtggtggatt tctttaacgg tataggcgga agccgatata tatttttagg gttagtggtg 960

ttgttctttt tgttcgtcgg aacctttatg gacgccattc cggcaatggt tttatttgta 1020

ccggtgattt atcccgtcgc acagtccatg gggattgatt ccgtccaatt aggtatcctg 1080

gtgatagtga cattgtcgat aggttagtc actccgccct acggtttatg tttgttgatc 1140

gccagtgtga tcggcgaatt gcctatcgat agatccttta aagccgtaat gccctatatc 1200

atgattattt tgctgacatt gtcattaatc gtttttgtgc cgcaaatttt actgtccgag 1260

actttacttt aa 1272

<210>95

<211>1008

<212>DNA

<213> Actinobacillus succinogenes

<400>95

atgaaaaaaa gaaccttctt gaaaacatta ttagcgagtg tgatgctgtt ttcactttct 60

gcctgcgatg ataacggcgg cagttcatca gctaaaattg tgaaagtttc ttccgttgtt 120

gccccgaccc acccttcgac cattgcgtta aaagaagtct tcaaacctat gattgaaagt 180

aaaactaacg gtaaatatca aatcgatatt tatgattccg ggcaattagg cgatgaaaaa 240

caatcctttg attacacccg ccaaggtatc gtggatatgt ccgtattggg gacggtgatg 300

tggtccgaag taccgaaaat gtccgtaccg gattttccgt ttttattcag agacgttgcg 360

catgcccgta atatttacca aggcgaggtc ggtcaggaaa tcgctgcgag ctttgaagaa 420

tcccaaccga ttaaattttt agcctggatg ccgaacggcg cccgagtttt ctcttccagc 480

aaaccgctgt ctaatccgga acaattcaga ggtcaaaaaa tgcgtatgcc gaacaatccg 540

attcacgtga aggtggcgga gctgttaggc gcgaacgtgg cgattatggg attaggcgaa 600

gtcttcaccg cacttgaaca aggcgtggta gacggtcagg acaatccgtt gtctactttt 660

gtgcagcaag gtttctattc cgtaaacaaa aatattttg aaaccaatca tatgatttct 720

tctttggaat tattagcaaa cgcgaatttc tggaaggatt taagtgatga agaaaaagcg 780

atctttaatg aagcggcgaa agcgacctcg gataaaagct gggatcttta tcagaacagt 840

atcgaagcgg ataaaaaatt agtgcaggag aaaggcggca caattaccgt tccgactgaa 900

gcggataaaa aggcgttagt ggaaaaaatg cagccgcttt atgacgagtt gtataaaaaa 960

tacgattggg ccgaagcttt agtcaataaa gtcagagata cccaataa 1008

<210>96

<211>987

<212>DNA

<213> Actinobacillus succinogenes

<400>96

atgaaattac tttctaaatc aatcaaagct atcctttcag tgggtttatt aggattagca 60

attaacgctc aagcagaaac ggaaatcatg gttgcttatg gtaaccagcc tggcgaacct 120

atcgataaag caatgcactt ttgggcggac aaagtcaaag aaaagtctaa tggcgacatc 180

gtatttaaat tgtttccatc gagccaatta ggtagtgaaa ctgaggtatt ggaacaggca 240

aaattcggag caaatattat tactatcagt gattacggcg cattaatgga tattgtgcct 300

gatcttggtg ttattaatgc gccgtatatt agccaatcat ttgagaaaaa atctaagtta 360

ttacattctg actggtttaa agattaagc gataaattag atcaacatga tattcatatt 420

atcgttcctg atgtaattta tggtacgcgt catttattaa caaagaaacc ggtaacaaaa 480

ccaagtgacc ttaaaggtat gaaagtccgt gttcaacatt cacgcttgtt cttacaaacg 540

attaacgcga tgggtggggt tccgacaccg atgtcattta ctgatgttta ccccggctta 600

tccgaaggga ttattgacgg tttagaaaat cctactgttg tattattcgg tgggaagttt 660

tttgaagtcg ctaaaaatct gaatttaact gctcataacta aacacatgtc tccatttgtg 720

gctggtacgg cattttggaa taacttaacg ccggaacaac aaaagattat tgtagatgca 780

agtcgtgaaa tggtgactta tggtggagga ttaattgaac aggctgaaaa tgaagcattg 840

gaaaatttga agaaagcggg tgtaacggtt aacgatgtcg atttgccggc tttcgaagca 900

tctgttgccg atgttatttc cacaggattc cctgaatggt caccaaatct ttataaaaat 960

gttcaggaaa aactttctca attttga 987

<210>97

<211>483

<212>DNA

<213> Actinobacillus succinogenes

<400>97

atgcaaaaaa ttattaatta catagaaaaa gtgcagttaa tactgggggc tatatttctg 60

gttatttttg ttatagccac cctgttgcag gtaagtacgc ggtatttggg gatcagcgct 120

acctggacgg aagaactggc ggttaatact tttatttggg cgatgatgct gggggcggcg 180

gtaatggtca gacaaaaaca gcattttagc ttcggttttc tggcatctta tctgtcggac 240

agaaaatccg cctttctgat tatttttcaa aacattattt taatcacatt ctgtgcatta 300

tgttttgtct acagcagtga aatcacccaa gcattttgga attcaaagtg ggtatctatt 360

cctcagttta aacaaggata cgtttggctg gttttaccta ttacttttat gaccatgata 420

atttatgtga tagaagactc aattaagcaa attaataaat taatacgtga taaaaattca 480

taa 483

<210>98

<211>492

<212>DNA

<213> Actinobacillus succinogenes

<400>98

atgtctaaac caacaattaa aaagaaattt tggctaagtg atattgatga aatattagca 60

tcatttttct tagcgttaat tgttctgtta tcgggtacg gcgtagttat gagatatttt 120

ttaaatactc ctagtgcctg ggtagaagaa atctgtgtcg tattttttat ttggtttaca 180

tttttggcat caagcgcctt atgtaaaaac aatgaattga ttcgcattga ttatctatta 240

actaaaattc cgaacaagat agcaaatttc atagacggag tgattcaacc cttaatcatg 300

attttttgtt tgggattt gattattgg ggatttaaat tattaccaat gtcgaaaatg 360

cgtttcacac cggcattaca aatttcttat gtctatattt atgccatcat tccgattagt 420

gcatttatta tgctttatta tgaattaaga aaaattgctt attacttcaa acttagtaat 480

aagggggatt aa 492

<210>99

<211>477

<212>DNA

<213> Actinobacillus succinogenes

<400>99

atgctggaac aatatgagaa atttatcaat aaaacctttt tatcattagg atgtcttttt 60

tttcttgtgt tagttgcgat gacatttgta caggttatta cgagatatgt gattaacatt 120

tcgtttccct gggcggaaga gtttttgaga ttttgctata cctgtatgat tttattcgga 180

attttaacga caacccagat tcaggttcct atggttagaa tcatgatttt tgagcgattt 240

aaatacggct ggttattatc ctccgctcta tacattgtga tgttaatttg tttatttta 300

ttagttaaag gcggtttaaa agtttattcc atgaatttga atagctatta ttcggcgttt 360

aagatctcaa agtcttgggt atatataccg tttcttattt gtttatcatt agagatggtg 420

cgaattgtca ttgcgttcgt ttctttagct aaaaccagaa aagtgaggga attataa 477

<210>100

<211>510

<212>DNA

<213> Actinobacillus succinogenes

<400>100

atgaaaactt ttaccgataa attgttcaac ggtattcaaa tattgctggg tgtgatgctc 60

gccgtaatga ttgcccttgt gttttttaac gtcatacttc gattcttttt caattccggt 120

ttggtttggt cggaagaggt ctcgagatat gtttttgttt acgtgatta tctcggggcg 180

attgtggcga tgaaggaaaa tgcccatttg ggcgtggata cgttcattaa aaacgcaccg 240

gaaaaattga aatggctgct tttcgtcgtt ggacgggtca tcattattgt cgttatggct 300

atcttgttta aaggtagcct ggctatggtg gttcaatcgc atacggcgaa agcggcggca 360

acgggtttgc ctttatccgt tgtgtacgga atcggattat tgacgggagc ggcaattatt 420

ttaattgcgt tactgaatat tattgatgtg attagaaatc ctaaatctgt tcaccaagcc 480

gcattgatga gtgaagcaga ttcagattag 510

<210>101

<211>492

<212>DNA

<213> Actinobacillus succinogenes

<400>101

atgaccaggc tcatatccct attagaccgt atactggccg ctttctgtat tgtgctgtgt 60

gcggcgctcg tcgtttctgt cgtatggcag gttttttcac gttatgtact gaatgatccg 120

agtacggtaa cggacgaatt ggcacgcttt ctttttattt gggtcggatt agtcggagcg 180

gcttacggat tagggaaaaa gaaacacctt gccattgatc tgttgttgat gaaactcgaa 240

aaaagcccta aaaaacaggc atttcttcaa ctggttatca atactatcag tatatttttt 300

attgtcgtaa tcatgtgtta cggcggaatg aaactggttc tcgatacgat agccgccggt 360

caggtctctc ccgtattggg aatccaaatg ggattggttt atctggcgct tcccgttagc 420

ggattcttta tgctcgtctt tttagtgcgg gattttatcg ctgacattcg tcagttttca 480

ggtcagaatt aa 492

<210>102

<211>483

<212>DNA

<213> Actinobacillus succinogenes

<400>102

atgaatgctt tagcccattt tgtgaaaaaa gccattgaag tcatttgcgt gataattctg 60

tctgcgatgt ctatcttggt ttttgtgaac gtcgtattac gttacggctt taacagtagt 120

ataaacatta ccgaagaagt gtcccgttat atgtttgtct ggctggcttt cttaggtgcc 180

gtgctggcat ttaacgacaa tcaacacgtg agagtaacgg tttttgtcga aaaactgtcg 240

ccggttgcgc gcaaattgct gcatattgtc acggatgccg cgatgttgtt ttgctgttat 300

ctgattgtag acggcagctg gctccaattt aatctgaatc tgaacaacct cgcaccgatt 360

tccggtttgc cgcaaggtat cacttatctc gccagtacca ttgccggtat gttaatcggc 420

attcttatca tagcgcgtat gataaccggt attttcacct taaataaggg agaagcaaaa 480

tga 483

<210>103

<211>978

<212>DNA

<213> Actinobacillus succinogenes

<400>103

atgaaaaaat taactgttat ttcagcaacc gtattacttg ctatgttctc tgcgtctacc 60

tttgccgcgg gagaacatac gttggcgctt agtcactatg gttcgccgag cgataccgtg 120

acaaaagcaa ccgcgttgat ggccaaaaga gccaatgaac tgtctaatgg acgtatcacg 180

attaaattac atcccaatag tgaattgggg gcttccgata ctcaaatcga cggtaccaga 240

atggggacga ttgatattgt tgtagtagga aacccgtatt acaccacctt taatgaagaa 300

ctcaatctat tggatttacc attcctattt aaagacgaag ctcatgttga aaaagtatta 360

aatggggaag tcggggaaca tctgttgaaa tcattggaaa gtgcaaactt gaaagggttg 420

gcattctatg agattggatt tagagatatc actaacagca aaaaacaaat taaaaccgtc 480

gcgaatttat ccggtctgaa gttaagaacc actcctaatc ccgcacatat cgcggctttt 540

aaagaatggg gtgcaaatcc gacgccaatg ccgtttaacg aagtttattttctctgaaa 600

accggcgtta ttgatggaca ggaaaatccg gttcatcata tttacaacaa taacttacag 660

gaagttcaga aatatctgtc tttaactcat catgcttata ccgcggcacc aatgtcaatg 720

aatttggatc gctttaattc tttagataaa gaagatcagg atattttgtt gcaagcggca 780

aaagagggcg ctgagttaga gaaacaactg aatgtggaag aaaataaggt ttatttggat 840

aaactaaaac agcaaggtat ggacgttgag gaagctccgg atacagcctc tttccaagcc 900

ggtgctaaaa aagcctggga tgattatgcc aagaaatttg gtgatcaaat gattaataaa 960

gtgactgcca ctaaataa 978

<210>104

<211>987

<212>DNA

<213> Actinobacillus succinogenes

<400>104

atgtttatga agaaaaatgt ccttacccta gcgataagcg gcttagtggc ggcgacgatg 60

tccctttccg tgtcggccaa aacaacgtta aaattaagtc ataacaatga taaaacacac 120

ccggttcata tttctatgca ggctatggcg gatgaagtca aaaaagcaac tgacggtgaa 180

gtggttgttc gtatttatcc gaacagtcaa ttaggtaatc aacgcgaatc catggaatta 240

attcaatcgg gttctcttga tatggcaaaa accaatgcca gtgaattaga agcgttcgaa 300

cctgtttacg gtgcgtttaa tgttccgtat ttattaagg ataccgaaca ttattacaaa 360

gtactgaaag atcctgaaat cggcgggaaa attctggatt ctacaaaagg caaaggattt 420

gtcgggttaa cttattacga tgcgggatcc cgtagttttt acgctaaaaa gccgattaaa 480

actcctgcgg atttaaaagg gctgaaagtt cgcgtacaac ctagtccgag tgcattggaa 540

atgatgaaat taatgggggc gtccgcaaca ccgttggcat tcggtgaact ctatactgcg 600

cttcaacagg gcgtcgtaga tgcggcggaa aataacccga cggccttaac attaatgcgt 660

cacggtgaag tggcgaaatt ttatagcgaa gacgaacaca caattatccc ggatgtcttg 720

ttaataagtg aaaaatcctg ggcaaaactc actccggaac agcaaaaaat agtgaaggac 780

gcagcggata attcaatgat gagccataaa gatctttggg ctaaggcaac tgaagaagaa 840

attcagaaag caaaagatac gatgggagtt gagttcgtaa aagtggaaaa acaaccgttt 900

gtggatgcgg tgaaaccgat gcatgataag gcgcttgccg atccggtcat tggtccgatg 960

gtacaaaaaa ttgatgccgc acgataa 987

<210>105

<211>966

<212>DNA

<213> Actinobacillus succinogenes

<400>105

atgtcagttg ttctcgccgg ttgtaatgat tcggggacag ataaccggac ggtgaaaatt 60

gttgccgcac ataatcaaac caatatcacc agcccgtacc aatcgggtat gaataaactt 120

aaagaagcgg ccgaagccga taagcagttt gaagtggagg tacacgccgg aaccatcggt 180

acaaacgaaa acgaacttgt cgaaaaattg gttttaggcg gcgcggatat cgtgttggtg 240

tctccgggct tcatgacttc ggcaggcatt aatgaagtgg atttattctc tctgttatat 300

ctgttcaatg attataagca ctgggaagcg gtagttgacg gtgaaatagg taaaaaactg 360

tccgatatta tttatgaaaa atcaggtaat aaataccgcg tcatcgcata ttggagtgcg 420

gccgtccgtc attggtacgg taaaagaccc attaatagtt tagccgatgc gaaaggatta 480

aaaatcagaa cgcagacttc aggcgtggtt tccgattttt ggtcggccgt cggggctatt 540

ccgacttccg tcgcttgggc ggaactttat caggcgttag ccagtaatgt ggtggacggc 600

gccgaaaatg cttatccgta tcttgtgccg atggaacatc acaaaaccgc aaacggtaaa 660

tttatttccg aaaccgccca tgattatacc acccgacttc tgttggtaag agcaagtacg 720

tgggataaat taaccgacga acaaaaaacg gtattgcaaa acgcggttca ggaagcgact 780

aaagcggaaa gaaacgccat ttataccgaa gaatcccagt ataaagagaa agcaatcgct 840

gacggcgcgg tggtcaatac gccggatgtg aaaccttttg ccgagattgc gacaactatt 900

caagacaaat gggcaaaaga caataacatg acggatattc ttgaaaaaat cagagctatc 960

cgataa 966

<210>106

<211>987

<212>DNA

<213> Actinobacillus succinogenes

<400>106

atgaaacttt ttaatttaaa aacattagcc gcattggttg caggtatcgc cgtaatgtcc 60

ggttctgcat ccgcggaaac ctcattccgc ttcggttatg aagcgccgcg ttccgatact 120

caacacatcg cggccaaaaa atttgcagag ttacttaaag agaaaactaa agatgaaatc 180

aaattaaaac ttttcccgga cagtaccttg ggaaatgcgc aaactatgat tagcggcgtg 240

cgcggcggca caatcgatat tgaaatgtcc ggctcgccga actttaccgg cttggaaccg 300

aaactgaacg ttatagatat tccgtttat ttcaaagacc gcgaccatgc gtttaaagta 360

ttggacggcg aaatcggaca gggtttgtta aaagacttgg aagcgcaagg cttaaaaggg 420

ttggcgtact gggatgtggg attccgtgct ttctctaact ccaagcactc cgtaagcaaa 480

ccggaagata ttaaaggctt aaaagtgcgt accaaccaaa acccaatgta tatcgaagcc 540

tttaccctgc taggcagcaa cccggtaccg atgccgttat ccgaactata caccgccttg 600

gaaacccgtg cggtggatgc gcaggaacat ccggtgggta ttttctggtc gtccaaactg 660

tatgaagtac aaaaattctt aagtttgacc aatcacggct ataccccgtt aatcgtggtg 720

atgaacaaag cgaaattcga cgctttatcg cccgaactgc aacaagccgt tcttgaatct 780

gccaaagagg cgggcgcata tcaacgcgaa ttaaacatca aaaatgaaaa agaaatcatt 840

gaaaatgtac gtaaagaagg cgtagaagtg accgaacagg ttgatcaagc gccgttcaaa 900

gcggtaattg aagaaaaagt acgtaaaacc tttatcgaca aatacggtaa agagttagtg 960

gaacaaatcg acgctttagc gaaataa 987

<210>107

<211>990

<212>DNA

<213> Actinobacillus succinogenes

<400>107

atgaaactat tttctcttac aaaactgacc gcacttatcg caggtatgac cttattttcc 60

gccgtatcgg cgcaagcgga aaccgcgtta cgtttcggtt atgaagcgcc gcgcagcgat 120

acgcagcaca tcgccgccaa aaaatttaac gatctgttaa aagaaaaaac cagcggtgaa 180

atcaaattga atttattccc cgacagcacc ttaggtaacg cgcaaaccat gatcagcgcg 240

gtacgcggcg gtacggtaga tttggaaatg tccagttcgt cgaacttcac gggattggtt 300

tcggcattaa atgtcattga tattccgttt atctttaaag accgcgctca tgcttacaaa 360

gtgttggacg gtgaaatcgg tcggaattta ttagcccaat tagatgcgca cgggttgaag 420

ggaatcgcct tctgggaagt gggtttcaga ggatttacca actccaaaca tccggtcacc 480

aaaccggaag acattaaagg cttaaaggtc cgcaccaatc aaaatccgat gtacattaaa 540

gccttcagta ttttaggcgc caatccggtg ccgatgccgt tatccgagct ttacaccgca 600

ttagaaacca aagcggtaga tgcgcaggaa catccgatcg gtatcgtttg gtcgtccaaa 660

ctgtacgagg tgcaaaaata tttcagcttt accaaccatg gctacaccccc gttaatcgtg 720

gtgatgaaca aagcgaaatt cgatgcgctt tcgccggaac tgcaaaaagc tattgtggat 780

gccgcagagg aagcgggcaa atatcaacgt cagcttaatt tagacaacga acagggtatt 840

gttgaaaaaa tgaaaaaagc cggcatcgaa tttgtagata atctggatac cgcaccgttt 900

aaagcggcgg tggaacagga aacccgcaag gcctttattg atgcgaacgg cgacagccta 960

atcaaacaaa ttgacgcttt aggcgaataa 990

<210>108

<211>567

<212>DNA

<213> Actinobacillus succinogenes

<400>108

gaagcttccg ctattgccgt ggtttacacc ttcgtattgt ccgtattgat ttatcgggaa 60

attaaattaa ccgcgttacc gaaaattatt ttggatgcca tcgtcaccac ctctatcgta 120

tgtttcctca tcggcgtatc ggttgctatg tcttgggcga tgactaacgc cgacattcct 180

tacatggtaa atgattatt aatcagcgta tcggataatc ttattgtcat tttattgatt 240

atcaatgtgt tattgctggt tatcggtatt tttatggaca tgacgccggc ggtcttaatc 300

tttaccccaa tctttttgcc gatcgtaaaa gatttaggca tggatcccgt tcactttgga 360

attatgatga ttttcaatct ctgcatcggc ttatgtacac cgccggtagg aagcgcactg 420

tttgtcggtt gttccgtatc agggggtgaaa ttacaggatt taatcaaacc gatgttacct 480

ttctttgccg tcttggtcat ctcattattg cttgtaacgt atgtaccgca attatcgtta 540

ttcctgcctc gtttgttagg actttaa 567

<210>109

<211>1281

<212>DNA

<213> Actinobacillus succinogenes

<400>109

atgaccattc ttattttttt agccagcctt gtaggcggta ttgtaatcgg tgtaccgatt 60

gcattctcgc tgctgttttc aggcatgatg ctgatgtttt atctgggcgg cttcaattca 120

cagattcttt cgcaaaatct gtttaacggt gcggacagct tttctatgat ggccattccg 180

ttctttgtgg ttgccggcga tttgatgaac cggggcggat taaccaacaa aatcgttgat 240

gcggcgaccg cacttgtggg gcatatccgg ggcggtttag ggtatgtggc gattatcgct 300

attctattgt tcgccagttt ggtgggaagc gcggtggcgt ctactgccgc tttaggggca 360

attttaattc cgatgatggc gactatcggt tacaaccgtg atcgcgctac cgcgttgatt 420

gcggcgggta atattttatc gccgattatg ccgccgtccg tgccgatgat tattttcggc 480

gttaccgcgg gcgtatccgt aacggattta tttatgggcg gtattgcacc ggccgtttat 540

attacactga ctctttgtgt gatttgggct atcgtttccc gtcgcgattc cgccgtaccg 600

ttgccgaaaa gatcattccg cgaagtggta cgcgctacgc gtgaagcgct ttgggcgatt 660

ttgatgccgg tcggtctttt attaggcttg cgtagcggta tgtttacgcc gacggaagcc 720

ggggtggttg cggttgttta tgcgttaatc atcggtttgt ttgtgtaccg agaattaact 780

ttccaagcca ttaaagaatg tttcatttct tccgcgaaag ccagtgcggc aattatgttt 840

ctggccgccg ccgcgatggt gtcttcctgg ctgatgacca tcggtaatat tccacgtatt 900

gtcacggatt tacttcatcc gtttatcggt tcgccgattc tgttaatgct tgtgattgcc 960

ggtttagtgt tgctggtggg aacttcgatg gacgtggtac cgactatttt gattttaaca 1020

ccggttattaa tgccggcagt gaaacaggcg ggaattaatc cggtctattt cggggtggta 1080

tttattatta cctgcgtctt cggtttgtta acgccgccgg taggtacggt attaaacgtt 1140

gccgccagta cgggtaaaat caacatggaa cgtatcgtgc gcaaagtatg gcctttcctg 1200

cttgcgattg ttgtggtgct gctcgtgtta atcgtattcc cgagtacgtt catgatacca 1260

cttgaatttt ttactaaata a 1281

<210>110

<211>1302

<212>DNA

<213> Actinobacillus succinogenes

<400>110

atggaatgga caacaattct cacattagcg ggcagttttt tctttttcct tgccatggt 60

gtaccaattt ctttcgctat cggcgtatca tcgcttttaa ccattttgct tgcccttccg 120

atggaatcgg caattaccgt tatttctcaa aagatggcat cgggcttaga cagtttttca 180

ctgctagcca ttccgttctt tattttggcc ggtaacatta tgaaccgcgg aggtattgct 240

ttacgtttaa tcgaatttgc caaagtattg ggcggtcgct tgccgggttc gttagcgcat 300

gtgaatgtac tcgccaacat gatgttcggt tccatttccg gttctgcggt ggcagcggcg 360

gcggcagtag gcggcacaat ggcgccgctg caaaaaaaag aaggttatga tccggcgttt 420

tccgccgcag taaatattac ctcttcgccg acgggtctgt taattccgcc gagcaatact 480

tttatcgttt actcattgat ttctggcggc acatcaatcg gcgcattatt tttagcgggc 540

tatattccgg gaattttaat gggtttgggt attatgagca tcgcctattt tattgcgaaa 600

aaaaacaaat atcccgtttc accgaaaccg acttttaaag aagtgacgca tcgtacttta 660

gacgcattgc cgagtttagg gctggtggtc gttatcatcg gcggtattat tgccggtatt 720

tttaccgcaa cggaagcttc tgcgatcgcc gtagtttata ccctgattct gtccatgatt 780

atttataaag aaatctcgct gaaagaatta ccccggatta ttctggatgc catgaccacc 840

acctctatcg tattactgtt aatcggtgcc tcaatgggaa tgtcctgggc aatggcgaat 900

gcggatattc cttataccat cagcgatgcc ttattaagcg tttcggacaa tccgattgta 960

attttattaa tcattaactt aacattatta atcgtgggta cctttatgga tatgacgccc 1020

gccctgttaa tctttacgcc gattttctta ccgatcgtaa ccgaattagg catggatccg 1080

gtgcatttcg gtattttaat ggcatttaac ttatcaatcg gcatctgtac gccgccggta 1140

ggctcaactt tatttgtcgg ttgttccgta gccggcgtaa aaatcgataa agtcatcaga 1200

ccgttactac cgttttacgc tatgttaatt ttaaccttat ttttggtgac attcgttccg 1260

cagttaagct tatggttacc gcaaacatta cttggctatt aa 1302

<210>111

<211>477

<212>DNA

<213> Actinobacillus succinogenes

<400>111

atgctggaac aatatgagaa atttatcaat aaaacctttt tatcattagg atgtcttttt 60

tttcttgtgt tagttgcgat gacatttgta caggttatta cgagatatgt gattaacatt 120

tcgtttccct gggcggaaga gtttttgaga ttttgctata cctgtatgat tttattcgga 180

attttaacga caacccagat tcaggttcct atggttagaa tcatgatttt tgagcgattt 240

aaatacggct ggttattatc ctccgctcta tacattgtga tgttaatttg tttatttta 300

ttagttaaag gcggtttaaa agtttattcc atgaatttga atagctatta ttcggcgttt 360

aagatctcaa agtcttgggt atatataccg tttcttattt gtttatcatt agagatggtg 420

cgaattgtca ttgcgttcgt ttctttagct aaaaccagaa aagtgaggga attataa 477

<210>112

<211>1539

<212>DNA

<213> Actinobacillus succinogenes

<400>112

atgacgaaca acttatcaaa acaacctatc ggctcggaat atctcagagc ggtattaagt 60

tctaaagtgt atgacttggc gcaagtcacg ccgttgcagg ctatgccgaa attatcggaa 120

aaactggaaa atcggatttt tgtcaaacgg gaagaccgcc agccggtgca cagtttcaaa 180

ttacgcggag cttacgccat gatcgcgggg ctggtgccgg aacaaaaagc ggcgggtgtt 240

attaccgctt ccgccggcaa tcatgcgcag ggcgtggcgt tatccgccaa aaacgccggt 300

attcgcgcgt taatcgtgat gccgcagaat acgccgtcca ttaaggtgga cgccgtccgc 360

gctttcggcg gagaagtgtt gttgcacggc gccaactttg atgaagccaa agccaaagcg 420

ctggagcttt ccgcaagcga acatatgaca tttattccgc ctttcgatca tcccgccgtg 480

atagccggtc aggggtctat cggtatggag attattcagc agaacagccg tgtcgatcgc 540

gtcttcgtgc cggtgggcgg cggcggtttg gcggcgggga ttgccgtgct gatcaaacaa 600

ttaatgccgg aaattaaagt aatcggtgtg gaagcggaag attccgcctg tctgaaggcc 660

gcgctggaag cggggaaacc ggtggatttg gaacgcgtcg ggctgtttgc ggacggcgtg 720

gcggtgaaac gaatcggtga cgaaactttc cgcgtttgcc ggcaatatct ggatgatgta 780

ataaccgttg acggcgacga aatctgcgcg gcgatgaaag atatgttcga aaatgtgcgt 840

gcggtgtccg aaccgtccgg cgcgttgtcg ctggcgggct tgaaaaaata cgtgaaaacc 900

catcgcatca aaggcgaaac gctggtgaac gtgctttccg gcgccaacct gaatttccat 960

actttgcgtt atgtgtccga acgttgcgaa atcggcgaac aaaacgaagc tttatttgcc 1020

gtcaccattc cggaacagcg gggcagcttc ttaaaattct gtcagatttt aggacaaaat 1080

gcggtgacgg aatttaacta tcgttatacg gacgaactgc aggcgcgcat ttttgtggga 1140

gtgcggatta ccggcggcga agaaaaagcg atgattctga cgcaactgaa acagggcggt 1200

tatgacgtgc aggattttc cgatgacgaa atcgccaaaa cccatgtgcg ttatatgatc 1260

ggcggacgct cgtccagtct gcttaaggag cgactttaca gtttcgaatt tccggaacag 1320

aaaggcgcgt tgttaaaatt tctggaaacc ttgggtacca ccaaagccaa tatttcgctg 1380

ttccattatc gcggacacgg cgcggattat ggcgatgtgc ttgccggttt ccaaatcgaa 1440

gacagtgatt tacgggcgtt taacgagcac ttgcaagcac tgggttacgg ttatcaggat 1500

gtcacggaca gcccggctta ccgctatttt ctgctgtaa 1539

<210>113

<211>1269

<212>DNA

<213> Actinobacillus succinogenes

<400>113

atgttagaaa agaaaaccat tgcggaatta gacccggttt tatgggacgc catgcaaaat 60

gaagtacgtc gccaggaaga acatattgag ttaatcgcgt cggaaaatta cgtcacgccg 120

gcagttatgc aagcgcaagg ttcccagtta accaataaat atgccgaagg ctatccgggt 180

aaacgttact acggtggttg cgaatatgtg gatatcgtgg aacagttggc tattgatcgc 240

gcaaaagagt tattcggtgc ggaatatgcc aatgttcaac cgcattcggg gtcgcaagcg 300

aatgctgccg tatatggggc attgttaagt gcgggcgata cgattttagg gatggatttg 360

gcccacggcg gtcacttgac ccatggtgcg aaagtgagtt tttccggtaa aatttataat 420

tccgtccttt acggtattac tgctgacggc gtgattgact atgccgacgt acgtacaaaa 480

gccttagaaa gcaaaccgaa aatgattgtt gccggtttct ccgcctattc ccaagtaatc 540

gattgggcta aaatgcgcga aattgccgat gaagtggatg cctacctgtt cgtggatatg 600

gcgcacgttg ccggtttaat tgccgcaggt ttatatccga atccgttacc gcatgctcat 660

gttgtcacga ctacaactca caaaacttta gccggtcctc gcggcggttt aattttatcc 720

gcctgcggcg atgaagatat ctataaaaaa ttaaacagtt ccgtatttcc ggcaaaccag 780

ggcggtccgt taatgcatgt gattgcggcg aaagcggtat gcttcaaaga ggcattggaa 840

ccttcattta aagtatatca ggctcaggtt ctgaaaaatg ccaaagcgat ggtggaagtt 900

ttcaaacaac gcggttttga cgtggtttcc aacggcacgg aaaatcattt gttccttgta 960

agcttcatta aacaaggttt aaccggtaaa caggctgatg ccgcattagg cgcggcgaat 1020

attacggtga ataaaaattc cgtcccgaac gatccgcaaa aaccgtttgt cacttccggt 1080

atccgggtgg ggtcgccttc aattacccgt cgcggtttca gcgaaaccga tgcggcaact 1140

ttagccggct ggatgtgtga tgtattggaa agtatcggca aagacaatca cgaacaagtg 1200

attgccgaga ctaaaaccaa agtgttagac atctgcaaac gcttaccggt ttacggtatt 1260

ggacgttaa 1269

<210>114

<211>639

<212>DNA

<213> Actinobacillus succinogenes

<400>114

atggcttaca gcaccgaaca aattatcgaa aaactcagcc gactgaaagt ggtcccggtg 60

atagccgtgg atgaggcgaa ggacattatt cccttagtgc aaaccttatc ggacaacggt 120

ttaccggtgg cggaaatcac cttccgttcg gcggcgggtg aagaagcgat tcggttaacc 180

cgtcagcatt ttcccgacgt actgattgcg gcaggaaccg tgctgacccc ggcacaagcg 240

gtggcggcta aaaacgccgg cgcggattgc attgtgacgc cgggattaa cccgaatatc 300

gtcagacttt gccaagcgtt ggacttaccc gttacgccgg gggtcaacaa cccgatggcg 360

attgaagcgg cattggaatt aggcgtgaag gcggtgaaat tcttcccggc ggaagccagc 420

ggcggggtga atatgattaa agcgttgctc ggaccttatg caaacctgca gattatgccg 480

acgggcggca tttcgccttc gaacatcaaa gattatctcg ccgttccgaa cgttgtggcc 540

tgcggcggtt cctggtttgt ggataaggcg ctgattaagt cgcagaactg ggatgaaatc 600

gggcgcttgg tacgcgaagc ggtggaattg gtcagctaa 639

<210>115

<211>639

<212>DNA

<213> Actinobacillus succinogenes

<400>115

atggcttaca gcaccgaaca aattatcgaa aaactcagcc gactgaaagt ggtcccggtg 60

atagccgtgg atgaggcgaa ggacattatt cccttagtgc aaaccttatc ggacaacggt 120

ttaccggtgg cggaaatcac cttccgttcg gcggcgggtg aagaagcgat tcggttaacc 180

cgtcagcatt ttcccgacgt actgattgcg gcaggaaccg tgctgacccc ggcacaagcg 240

gtggcggcta aaaacgccgg cgcggattgc attgtgacgc cgggattaa cccgaatatc 300

gtcagacttt gccaagcgtt ggacttaccc gttacgccgg gggtcaacaa cccgatggcg 360

attgaagcgg cattggaatt aggcgtgaag gcggtgaaat tcttcccggc ggaagccagc 420

ggcggggtga atatgattaa agcgttgctc ggaccttatg caaacctgca gattatgccg 480

acgggcggca tttcgccttc gaacatcaaa gattatctcg ccgttccgaa cgttgtggcc 540

tgcggcggtt cctggtttgt ggataaggcg ctgattaagt cgcagaactg ggatgaaatc 600

gggcgcttgg tacgcgaagc ggtggaattg gtcagctaa 639

<210>116

<211>1533

<212>DNA

<213> Actinobacillus succinogenes

<400>116

atgctaattg gtgtaccaag agaacggctc aacggtgaaa ctcgcgtggc ggcgacgccg 60

aaaaccgttg aacagattaa aaagctcggc tttgatgtca tcgtcgaaaa cgatgccggc 120

tttaaagcga gttttgagga taacgccttt accgcagccg gtacgtccgt gggttcccaa 180

caggatgtct ggaatgcgga tattattttt aaggtgaatg caccgacgga tgaggaaata 240

gcgctgatga aagacggcgc aaccttagtc agctttatt ggccggcgca gaatccgcag 300

ttaatggaaa aattgtcggc gaaaaaaatt aatgtgctgg caatggatgc ggtaccgcgc 360

attcccggg ctcaggcgct agatgctttg tcttccatgg cgaatatttc aggttatcgc 420

gccgtcattg aagcggcgaa tgccttcggg agtttcttta ccggacaaat cactgcggca 480

ggtaaagtgc cgccggcgaa agtgctggtg attggtgcgg gggtcgccgg tttggcggct 540

atcggcgcgg caaacagttt gggtgctatc gtacgtgctt ttgactctcg tcccgaagtg 600

aaagagcaag ttaaatccat gggcgccgat ttcctggaaa tcgatttcga agaagaaggc 660

ggttcgggcg acggctatgc taaagtgatg tcggaagaat ttaaccgtcg tgccatggca 720

ctttatgcgg aacaggcgaa ggacgtggat attatcatta ccactgccgc tattccgggc 780

aaaccggctc cgcgtttaat taccaaggaa atggtggatt ccatgaaacc gggtcggtg 840

gttgtggatt tagcggcgtt aaccggcggt aactgcgaat ataccaaagc gggtgaagtg 900

gttgtcacgg aaaatcaggt aaaagtcatc ggttatacgg attttccggc acgcctgccg 960

accccagtctt ctcagcttta cggaacgaat ctggttaatc tgttgaaatt actggcgccg 1020

aataaagacg gtcaaatcga tattaatttt gaagatgtgg tgttgcgtgg cgtaaccgtt 1080

attcgcgacg gtgaagtgac ttggccggct ccgccgattc aggtttccgc tcaaccgcaa 1140

caaaaagcgg cggcggctcc ggtggagaag aaagaaaaag aaccgacgaa tccgcgggtg 1200

aaatacggta ttatggcggc aatcgtcgtg ctgttcctgt ggctggcggc agtgacgccg 1260

gcggcgttct tatctcactt cacggtattc gtgttgtctt gtgtggtggg gtattacgtg 1320

gtgtggaacg tcagtcatgc gttgcatacg ccgttaatgg cggtaaccaa tgccatttcg 1380

ggcatttatta tcgtcggggc ggtattgcaa attgcacagg gcagtttcta tgtcagcctt 1440

ctggcgttta tcgcgatttt agttgccagc attaacatct tcggcggctt caaagtcact 1500

cagcgtatgc tttccatgtt tagaaaaggt taa 1533

<210>117

<211>1452

<212> DNA

<213> Actinobacillus succinogenes

<400>117

atgtcagtag gtttagtgca agccgcatac attgtatcgg cattactgtt tattatgagc 60

ctggcgggtc tttctaaaca tgaaacggcg aaagcgggtt gctggtacgg tatcgtgggg 120

atgaccattg cgttaatcgc aacaattttc ggaccgcaaa ccgaaggtca gttctggatt 180

atcgtagcca tgattatcgg tgcgatcatc ggtattcacc gtgcgttaaa agtggaaatg 240

acagaaatgc cggaattggt cgcgattttg cacagctttg tgggtttggc tgccgtttta 300

gtcggtttta acagtttcgg gttgcatatc gaaccggaat ttgtggcgcc ggtcggtacg 360

gcattcgtca gtgaagcggc gcaacaagcg gcaaaagaag cctttgatgc ggaacaggcc 420

gtattaaata atattcacaa tgtggaagta tttttgggca ttttcatcgg ggcggtaact 480

ttcaccggtt ccgtagtggc attcggtaaa ttgcgcggta ttatcaattc caaagcactc 540

atgttgccgc atcgtcataa attgaattta gcggcattag tggtttcggc gttgctgatg 600

gcggcattct taaataatcc ggaaaatatt ttcccgattt tgttgatgac ggcaattgct 660

cttgcctttg gttggcactt ggtggcctct atcggcggcg cggatatgcc ggtggtggtt 720

tccatgttga attcctattc cggttgggcg gctgccgcgg caggtttcat gttaaacaat 780

gacttgctta tcgtcaccgg cgcactagtc ggttcttccg gtgcgattct gtcttacatt 840

atgtgtaaag ccatgaaccg ttctttcatc agtgtgattg cgggcggttt cggtaatgat 900

acggtttctg cttccgatgc agagcagggc gaacatcgcg aaacaacggc ggaagaagtg 960

gcggaattgc tgaaaaacgc cggttcggtc attatcacgc cgggatacgg tatggcggtt 1020

gcgcaagcgc aatatccggt ggcggaaatg acccaaaaac tgcgtgagcg cggtgtgaac 1080

gtgcgtttcg gtatccacccc ggtggcggga cgtttgccgg gacatatgaa cgtattattg 1140

gcggaagcca aagtgcctta tgatgtggtg ctggaaatgg atgaaatcaa cgatgatttc 1200

gaagataccg atgtggttct cgttatcggt gccaatgaca cggtgaatcc ggcggcattg 1260

gatgatccga acagcccgat tgccggtatg ccggtgctgg aagtatggaa agcgcaaaat 1320

gtgatcgtat ttaaacggtc aatggcggtg ggttacgccg gcgtgcagaa tccattgttc 1380

ttcaaagaaa acacccaaat gttattcggc gatgccaaag aacgtgtgga tgatattctg 1440

aaagcgctgt aa 1452

<210>118

<211>1332

<212>DNA

<213> Actinobacillus succinogenes

<400>118

atgaaaaata tcgtacttgt cggaggcggc gccggcggat tagagctggc gactttctta 60

gggcataagc tggggcgtaa aaaccgcgcc aatgttactt tggtggatcg caatcaaacc 120

catctgtgga agccgttact gcacgagttg gcaaccggtg tcttggatgc ggaaaccgat 180

gccgtaagct atcgcgccca tgcgtttaat catcatttca atttcgaaca gggttccatt 240

gcacggatag accgcaccaa caaatatgtg gaattggcgc cggtttacgg caaagagggc 300

gatatgatcg tagtggcgcg tcgtattcct tacgattact tggtgctggc tatcggcagt 360

aaatccaatg attttaaaac caaaggagtg gcggaaaact gcattttcct ggattcgccg 420

gatcaggcat tacgtttcca gcacaaaatg ttggaattat tcctgaagtt ttccgaaaat 480

aacgctttgg cggaaatcgg cgaagacgac agcaaacagt ctttggttca ggaaggtaaa 540

gttaatatcg caatcgtggt cggcggcgct accggcgtag aattatccgc cgaactgttc 600

aacgctaccc ggcacttgtc atcttacggt tacggcaaaa tcgacgcagg gcatttgcgg 660

gtgaccctga ttgaggcggg cgaccgttta ttgccggcat taccgcaacg catttccgat 720

tcggtgcgta aagaactgga agaattaggc attacggtga aaaccgacac catgattact 780

gaagctacta aaaacagttt gctgaccaaa gacggggaag aaatcaaagc ggatttaatg 840

gtttgggcag cgggcgtacg ggtgtccgcg atcactcagc aatttgacgg cctagaatta 900

aaccgcatta atcaaatctg tgtcaaaaac agtttacaga ccacggtgga cgacagcatt 960

ttcgctatcg gcgattgtgc gttcctgctg caaaaagacg gcacacccgt gccgccgcga 1020

ggtcaggctg cgaaccagat ggcaacggta tgcggacaa atattgtggc gttgttcgat 1080

aataagccgt tgaaaaattt cagttatttc gataaaggtt cattagtgtc cttatctaaa 1140

ttcaccgcgt tagggaacat tactacgggc aagaaaacct ctatgactat agaaggacgt 1200

ctggcgcggt tggcgtatat ttccctgtat cgcctgcatc agcaaaaatt acacggctgt 1260

tttaaaaccg ggctaatcat tcttatcgga cgtttaaacc gttttattcg tccgtcgttg 1320

aaactgtatt aa 1332

<210>119

<211>1191

<212>DNA

<213> Actinobacillus succinogenes

<400>119

atgtttgaaa atattaaagc ggcccctgca gatcctattc ttggcttaaa cgaagcgttt 60

aaaacggaaa aacacccgca aaaaatcaat ttaggtatcg gtgtttataa agacgctcac 120

ggtttcacgc caattatgaa agcggtaaaa gcggcggaaa ctcgtttat ggagagtgaa 180

aacagcaaga attacktatc tattgacggc gtacaaaaat ttaacgccct gactcaaacg 240

ctcctgttcg gcgctgattc cgacattatc gccgataaac gggcaagaac ggtacaaagt 300

ttgggaggta ccggcgcatt acgcattgcg gcggaattca tcaaacgcca aaccaaagca 360

caaaatgttt ggatttcaaa ccctacctgg ccgaaccata atgccatttt caacgccgtc 420

ggcatgacta tccgcgatta tcgttggtac gatgccgaaa aaaaagcctt agactgggat 480

aatttgctgg cggatttagc caacgttaat ccgggcgacg tcgtgttatt acacggttgc 540

tgccataatc ctaccggtat cgatccgaca ccggaacaat ggcgacaact ggcggaatta 600

tcaatgcaaa acggctggct gccgctgttc gattttgctt atcaggggtt aggcaacggc 660

ttggacgaag atgccatagg cttgcgaacc tttgcccaat gccacaaaga attgctgatt 720

tgcagttcct attccaaaaa cttcggtctg tacaacgaac gggtcggggc atttacatta 780

gtcgcggaaa attcagatat cgccgatacg gtgttaaccc aagtgaagtc tattatccga 840

actatttact ccaacccggc ttctcacggc gcttcaacgg tagcaacggt attagcggat 900

cccgcattac acgaacaatg gaataccgaa ctcaccgaaa tgcgtaaccg tattaaacaa 960

atgcgcaaca aactggcgga attattgcaa gaattaggcg cacaacagga tttcggtttc 1020

atcaccgaac aaaacggcat gttctccttc agcggcttaa cgccggaaca ggtggatcgc 1080

cttaaaaacg aattcgccgt ctacgccgtg cgctcgggca gaattaacgt cgcgggcatc 1140

acagaagaca acatccgcta tctgtgcgaa agcatcgtta aagtgcttta a 1191

Claims (46)

1. method that is used to produce from the chemical products of A.succinogenes C4-approach comprises:

(a) provide and comprise the gene knockout that one or more suppress the C3 enzyme or the genetically engineered A.succinogenes of modification;

(b) be provided for cultivating the growth medium of described genetically engineered A.succinogenes; And

(c) in described substratum, cultivate described genetically engineered A.succinogenes to produce chemical products from described A.succinogenes C4-approach.

2. method that is used to produce from the chemical products of A.succinogenes C4-approach comprises:

(a) provide can one or more C4 enzymes of overexpression genetically engineered A.succinogenes;

(b) be provided for cultivating the growth medium of described genetically engineered A.succinogenes; And

(c) in described growth medium, cultivate described genetically engineered A.succinogenes to produce chemical products from described A.succinogenes C4-approach.

3. a production comprises from the method for the chemical products of A.succinogenes C4-approach:

(a) provide and comprise gene knockout that one or more suppress the C3 enzyme or modification and genetically engineered A.succinogenes that can one or more C4 enzymes of overexpression;

(b) be provided for cultivating the growth medium of described genetically engineered A.succinogenes; And

(c) in described growth medium, cultivate described genetically engineered A.succinogenes to produce chemical products from described A.succinogenes C4-approach.

4. according to claim 1,2 or 3 described methods, wherein the described chemical products from described A.succinogenes C4-approach is selected from the group that is made of succsinic acid, fumaric acid, oxysuccinic acid, 5-amino-laevulic acid, 2-oxoglutaric acid, L-glutamic acid and aspartic acid.

5. method according to claim 1 is that succsinic acid and described one or more gene knockouts that suppress the C3 enzyme or modification are selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof from the described chemical products of described A.succinogenes C4-approach wherein.

6. method according to claim 2 is that succsinic acid and described one or more C4 enzymes are selected from by PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof from the described chemical products of described A.succinogenes C4-approach wherein.

7. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is a succsinic acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

8. method according to claim 1, wherein the described chemical products from described A.succinogenes C4-approach is a fumaric acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

9. method according to claim 2, wherein the described chemical products from described A.succinogenes C4-approach is a fumaric acid, and described one or more C4 enzymes are selected from by PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID and the group that constitutes thereof.

10. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is a fumaric acid, suppress described one or more gene knockouts of C3 enzyme or the group that modification is selected from pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and constitutes, and described one or more C4 enzymes group of being selected from PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID and constituting.

11. method according to claim 10, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses fumaric reductase.

12. method according to claim 1, wherein the described chemical products from described A.succinogenes C4-approach is an oxysuccinic acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

13. method according to claim 2, wherein the described chemical products from described A.succinogenes C4-approach is an oxysuccinic acid, and described one or more C4 enzymes group of being selected from PEPCK, malate dehydrogenase (malic acid dehydrogenase) and constituting.

14. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is an oxysuccinic acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by PEPCK, malate dehydrogenase (malic acid dehydrogenase) and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

15. method according to claim 14, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses fumaric reductase.

16. method according to claim 14, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses FURAMIC ACID.

17. method according to claim 1, wherein the described chemical products from described A.succinogenesC4-approach is the 5-amino-laevulic acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

18. method according to claim 2, wherein the described chemical products from described A succinogenes C4-approach is the 5-amino-laevulic acid, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof.

19. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is the 5-amino-laevulic acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

20. method according to claim 19, wherein said genetically engineered A.succinogenes can overexpression cause glycine synthetic enzyme.

21. method according to claim 19, wherein said genetically engineered A.succinogenes further comprises the modification to one or more glycine synthetic genes.

22. method according to claim 1, wherein the described chemical products from described A.succinogenes C4-approach is a 2-oxoglutaric acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

23. method according to claim 2, wherein the described chemical products from described A.succinogenes C4-approach is a 2-oxoglutaric acid, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof.

24. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is a 2-oxoglutaric acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

25. method according to claim 24, wherein said genetically engineered A.succinogenes further comprises the gene knockout that suppresses the 2-oxoglutaric acid desaturase or modification and can the overexpression 2-oxoglutaric acid: the acceptor oxydo-reductase.

26. method according to claim 1, wherein the described chemical products from described A.succinogenes C4-approach is a L-glutamic acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

27. method according to claim 2, wherein the described chemical products from described A.succinogenes C4-approach is a L-glutamic acid, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof.

28. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is a L-glutamic acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

29. method according to claim 28, wherein said genetically engineered A.succinogenes further comprises the gene knockout that suppresses the 2-oxoglutaric acid desaturase or modification and can the overexpression 2-oxoglutaric acid: the acceptor oxydo-reductase.

30. method according to claim 28, wherein said genetically engineered A.succinogenes can also the overexpression glutamate dehydrogenase.

31. method according to claim 1, wherein the described chemical products from described A.succinogenes C4-approach is an aspartic acid, and suppresses described one or more gene knockouts of C3 enzyme or modification and be selected from by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

32. method according to claim 2, wherein the described chemical products from described A.succinogenes C4-approach is an aspartic acid, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof.

33. method according to claim 3, wherein the described chemical products from described A.succinogenes C4-approach is an aspartic acid, suppress described one or more gene knockouts of C3 enzyme or modification and be selected from, and described one or more C4 enzymes are selected from by Succinyl-CoA synthetase, PEPCK, malate dehydrogenase (malic acid dehydrogenase), FURAMIC ACID, fumaric reductase and the group that constitutes thereof by pyruvate kinase, pyruvic acid-formic acid lyase, pyruvic oxidase and the group that constitutes thereof.

34. method according to claim 33, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses fumaric reductase.

35. method according to claim 33, wherein said genetically engineered A.succinogenes can also overexpression aspartic acid ammonialyase.

36. method according to claim 33, wherein said genetically engineered A.succinogenes further comprises the gene knockout that suppresses the 2-oxoglutaric acid desaturase or modification and can the overexpression 2-oxoglutaric acid: the acceptor oxydo-reductase.

37. method according to claim 33, wherein said genetically engineered A.succinogenes can also the overexpression glutamate dehydrogenase.

38. method according to claim 33, wherein said genetically engineered A.succinogenes can also the overexpression aspartate aminotransferase.

39. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises the modification that one or more is improved the gene of the flux that passes through EMP.

40. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises the modification that one or more is improved the gene of the flux that passes through PPP.

41. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses malic enzyme.

42. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises gene knockout or the modification that suppresses oxaloacetic decarboxylase.

43. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises the modification to one or more genes that improve the substrate picked-up.

44. according to the described method of claim 43, wherein said one or more genes improve the picked-up of substrate, described substrate is selected from the group that is made of pectinose, wood sugar, glucose, fructose and sucrose.

45. according to claim 1,2 or 3 described methods, wherein said genetically engineered A.succinogenes further comprises improving the modification of one or more genes of chemical products excretory.

46. according to the described method of claim 45, wherein said one or more genes encoding dicarboxylic acid transporters.

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