CN101264093B - Compositions for increasing energy in vivo - Google Patents

Abstract

The invention relates to a composition comprising ribose for increasing energy in vivo in purpose of preparing medicine composition orally to mammals with 0.1-20g ribose daily dosage for increasing mammals energy level. Precursors of adenosine triphosphate are administered orally to increase intracellular ATP concentration as dietary supplements or for treatment of reduced energy availability resulting from strenuous physical activity, illness or trauma. Pentose sugars are administered individually, mixed into dry food or in solution. The preferred pentose is D-ribose, singly or combined with creatine, pyruvate, L-carnitine and/or vasodilating agents. Additionally, magnesium, electrolytes, fatty acids and hexose sugars can be used. The compositions and methods of this invention are especially beneficial to mammals having reduced energy availability or high energy demand.

Description

Compositions for increasing energy i (in vivo)

Invention field

The present invention relates to effectively to increase compositions and the method for the low or mammiferous energy that level of power consumption is high of energy efficiency.Described mammal comprises suffering from the human body that reduces associated diseases because of adenosine triphosphate in cell (ATP), is engaged in the moving crowd of ass work as athlete or physical labourer, and wishes to improve the human body of its energy level.Other mammal is also included within the inventive method as Canis familiaris L. and cat.Give compositions of the present invention and can improve blood and intracellular ATP level, extend the time and intensity of mammal activity, and improve the utilization rate of exercise test person to oxygen.Also benefit from the administration of the present composition without motion mammal and those higher than the mammal of the energy of normal level in somatic damage (as wound, burn and sepsis) recovery period consumption.

Background of invention

The energy maker of known cell is adenosine triphosphate (ATP).In anabolic process, the energy that derives from the nutrient metabolism is transferred to the energy-rich phosphate bond of ATP.Energy in these keys is consumed in the stage at energy charge.Muscle contraction needs the consumption of important and " expensive ", wherein ATP Rapid Circulation.

During the energy accumulation step occurs in interior two elementary process of muscle cell.Oxidative phosphorylation replenishes ATP by Decomposition Cycle fatty acid, glucose and intramuscular glycogen and triglyceride.By phosphagen, circulating glucose and intramuscular glycogen, through kinase reaction, as myokinase, reaction provides ATP in the anaerobic phosphorylation.

U.S. Patent number 5714515 has been described to use and has been contained pyruvate, and a kind of compositions of middle catabolite of glucose can strengthen the recovery of tired due to operation or accident trauma, shock, long-term physical demands and other indication.U.S. Patent number 5709971 discloses other glucose metabolism product with nicotine adenine-dinucleotide, coenzyme A and acetyl group coenzyme A administering drug combinations, namely glyceraldehyde-3-phosphate, phosphoenolpyruvate and 3-phoshoglyceric acid.

A kind of different approaches that now has been used to effective substrate of increase production ATP is to give the aminoacid VBT, and this material is believed to strengthen fatty acid and transports and absorb in mitochondrion, and mitochondrion is the oxidative phosphorylation position.U.S. Patent number 4968719 has been described the purposes of VBT treatment peripheral blood vessel.

No matter the energy-rich phosphate bond of ATP is to generate at aerobic or under anaerobic, and generates substrate used regardless of it, if do not have the precursor of ATP molecule just can't synthesize ATP.The resynthesis of ATP molecule can by from the beginning or salvage pathway carry out.

In the synthetic ATP of nucleotide salvage, can be present in in-house nucleotide precursor be converted into AMP and further phosphoric acid turn to ATP.The adenine Direct Phosphorylation is AMP, and xanthine and inosine are at first by ribose 5-phosphate base-1-pyrophosphoric acid (PRPP) ribosylation and and then be converted into AMP.Find that ribose content in full diet is very low, synthesize by pentose phosphate pathway in body.In de novo synthesis, ribose phosphoric acid turns to PRPP, and becomes intermediate adenosine monophosphate (AMP) with the adenine condensation.AMP further becomes adenosine diphosphate (ADP) (ADP) and ATP through the energy-rich phosphate bond phosphorylation.

By from the beginning approach is slowly synthetic.Usually, AMP is synthetic mainly to be undertaken by salvage pathway, yet, after anoxia or ischemia, from the beginning the activity of approach increases.

In the energy charge process, ATP loses a high energy band becomes ADP, and ADP can be hydrolyzed becomes AMP.AMP and metabolite adenine, hypoxanthine and inosine in muscle cell can arbitrarily spread, but can not be used for by salvage pathway the resynthesis of ATP.

In U.S. Patent number 4719201, it is disclosed that the further metabolism of AMP is adenine, inosine and hypoxanthine when ATP is hydrolyzed to AMP in cardiac muscle during ischemia, and cell loses them when pouring into again.When AMP does not exist, phosphorylation can't occur again and obtain ADP and ATP.Because above-mentioned precursor is rinsed out cell, nucleotide salvage can't supplement the level of ATP effectively again.This patent discloses when ribose restores for ischemia in intravenous fluids is administered to heart, and the recovery of ATP level is increased.

Pliml finds that in German patent 4228215 oral ribose can effectively treat cardiac insufficiency and hypovolemia shock in human body.

The people such as Zolner (Klinische Wochenshritt 64:1281-1290,1986) proposed to give pentose as ribose or xylitol for suffering from patient's prevention skeletal muscle pain and the tetanic superiority of autosomal recessive hereditary diseases myoadenylate deaminase (MAD) defect.The feature of this disease is permanent muscular hypotonus, excessive muscle weakness, fatigue, misery, causalgia, tetanic and cramp.These symptoms are considered to the consequence that the ATP circulation is interrupted.The AMP accumulated has suppressed the dephosphorylation of ATP, and causing lacking affects muscle contraction and lax available energy.Yet, although lack MAD patient's symptom, by the ribose administration, can alleviate, the level of the interior adenylic acid of cell still maintains abnormal high-level, and Healthy Volunteers can't be benefited by the ribose administration.(Gross，Reiter & Zollnet，KlinischeWochenshritt，67：1205-1213，1989)。

The people such as Tullson (" U.S. physiology magazine " (Am.J.Physiol.), 261 (cytophysiology 30) C343-347,1991) incorporated by reference document proposes, and high-intensity exercise has increased degraded and the secondary loss of in vitro intramuscular AMP.They further disclose to adding ribose in infusion liquid can increase the de novo synthesis of intramuscular AMP of sitting quietly in rat back leg specimen, but can't eliminate the middle de novo synthesis that contracts muscles, goes down.

The people such as Carniglia disclose when the compound mixture that contains aminoacid, metabolite, electrolyte and ribose or ribose precursor gives horse racing as food additive is oral in U.S. Patent number 4871718, can improve intramuscular ATP level and body behavior expression effect.The assessment of behavior performance is anecdote, however the performance of the behavior based on this experimenter history.

Therefore, need all the time not to be in the mammal of time application the method because of ischemia in normal mammalian, before carrying out physical exertion or during improve the short-cut method of skeletal muscle behavior performance.Also need a kind of mammal energy level that improves so that the method for comfort to be provided.

Summary of the invention

The invention provides the compositions and the method that improve energy level in mammal.The compositions and methods of the invention are by promoting the synthetic of ATP to work so that the sustenticular cell function in the mammal under-supply at ATP.Particularly, before one period of high ATP demand, during and afterwards with the oral pentose that gives of the effective dose that can effectively improve the mammal energy as D-ribose.The mammal of ribose administration can be for more time movable than the mammal that does not give ribose, obtains more high strength, has more energy.

It is said that the concentration of cell PRPP is restriction factor in the ATP level by de novo synthesis or nucleotide salvage is recovered or increased, and the ribose given can promote ATP synthetic, for energy expenditure provides larger ATP deposit.The mammal of ATP demand deficiency comprises normal, the healthy experimenter of high energy demand, as athlete and the worker who carries out heavy physical labour.Also infer, normal experimenter even also will experience positive high comfort at resting state after giving effective dose ribose.

The effectiveness of PRPP may be controlled salvage pathway and the activity of approach from the beginning, and adenine is to the direct conversion of ATP.As if be subject to glucose-6-phosphate dehydrogenase (G6PDH) restriction by glucose production PRPP.Glucose is converted into ribose-5-phosphoric acid under the effect as G6PDH at enzyme and further phosphoric acid turns to PRPP, and it strengthens from the beginning approach and salvage pathway, and the utilization of adenine.Add ribose to walk around this speed limit enzymatic step.

Also comprise in experimenter that the inventive method is benefited and having because of chronic low energy level due to aging, wound, sepsis, or as the mammal of disease or other chronic diseases of congestive heart failure.

The compositions that strengthens the pentose benefit also is provided.Suitable magnesium, creatine, pyruvate, VBT, pentose, at least one of other energy metabolism products and at least one the optional vasodilator substance of containing of such composition.Wherein, ribose preferably share with creatine and magnesium.The mammal of standing high energy demand and loss body fluid also can be from further containing electrolyte and the additional source of energy compositions benefit as carbohydrate.

The accompanying drawing summary

Fig. 1 means the dose response of adenine salvage pathway in the Normal Adult Rat of ribose administration.

After Fig. 2 is illustrated in ribose or placebo administration, based on exoergic circulation (exercycle), measure the mean power output during the each all-out sprint of normal adult.

After Fig. 3 is illustrated in ribose or placebo administration, based on exoergic, circulation is measured, the power stage peak during the each all-out sprint of normal adult.

Detailed Description Of The Invention

The invention provides and a kind ofly by the oral pentose that gives, promote the synthetic method of ATP, and provide to be particularly useful for and stand the high energy demand or suffer from the mammiferous containing the pentose compositions of chronic low energy level.

For the present invention is described, following term has following implication:

1. " pentose " refers to monosaccharide, includes but not limited to ribose, D-ribose, ribulose, xylitol, the 5-carbon precursor of xylulose and any ribose.

" vasodilation " but comprise that any percutaneous or oral administration cause angiectatic material, comprise adenine, hydralazine, arginine and nitroglycerin.

3. " ATP level in cell " refers to the ATP concentration of directly measuring or indirectly measuring by blood ATP concentration by biopsy or nuclear magnetic resonance, NMR.

4. " other energy metabolism product and cofactor " refers to creatine, coenzyme class, the intermediate of tricarboxylic acids, pentose phosphate or glycolytic ferment approach, pyrimidine and purine nucleotides and inorganic matter.

Described compositions preferably contains dissolves or is dispersed in the pentose of water carrier as the energy raising amount in water, and said composition can contain less and the additive of effective dose, as polyhydric alcohol, antiseptic, correctives, coloring agent etc.What be suitable for oral administration also comprises solid dosage forms containing the pentose compositions, as tablet, lozenge, capsule etc.Pentose can also be blended in the solid nutrient, for example square strip food, dog food, powder or bland wet or drying.Effective accumulated dose of ribose is open hereinafter, can spread to thus other pentoses.

Because pentose is to have the natural sugar of pleasant taste and in fact nontoxic, the pentose that can encourage experimenter oneself to use tablet, lozenge, powder, suspension, solution form or mix with food.When the experimenter is Canidae or cat family, pentose is easy to be added into " gourmet food " or " cardiac diet " and not necessarily separately administration.When this experimenter is human body, pentose can be contained in beverage, square strip food, cocktail or fast food.Preferred pentose is ribose or xylitol.Dosage is suitably 0.1 to 100gm pentose/sky, preferably 1 to 20gm pentose/sky.Find into 4 to 8gm pentoses/sky for each person and be enough to provide beneficial effect of the present invention.Upper limit dosage only is subject to the restriction of experimenter's sense of taste preference, although dosage is very high, the experimenter may diarrhea.Can be with unit dosage forms administration every day 1 time, but preferably whole day administration 2 or 3 times, the most common during having meal or administration afterwards.

In hard activity, individuality may be sweat in a large number, needs to supplement body fluid and electrolyte.The experimenter who does not perspire loses large quantity of moisture and also needs liquid make-up through lung as Canis familiaris L..Except the superiority that pentose provides separately, for carnitine and or vasodilation, generally supplement in liquid and contain other components, in order to drink after the motor process neutralization.ORS is as Gatorade

, Thirst Quencher, and Max

beverage is liked by the athlete.

These lasting energy and anabolism formula generally are comprised of following material: different carbohydrates comprises corn syrup, sucrose, fructose and maltodextrin; Protein-based, comprise casein and other protein that derive from milk and Semen sojae atricolor; And lipid, comprise Semen Maydis, Semen sojae atricolor, Flos Carthami and White cinnamon (canola) oil and medium chain triglyceride.For the diligence that improves this type of " behavior beverage " is continuing.

U.S. Patent number 5292538 has been described energy and has been maintained compositions, the magnesium that said composition contains fructose, glucose, hydrolyzed protein and coordinates with amino-acid chelate.Other useful especially components comprise potassium, phosphorus, manganese, zinc, boron, copper, molybdenum, chromium, vanadium, vitamin B _1,2,5,6,with ₁₂, vitamin C, E and carnitine.

U.S. Patent number 5114723 has been described the low infiltrated drink compositions given for oral, comprises electrolyte, inorganic matter, carbohydrate and other components.Said composition is adjusted to 100 to 70mOs/l Osmolalities.

These rehydration beverages can be respectively by adding approximately 1 to 20% pentose, and first-selected 10% pentose (weight/volume) improves.The addition of pentose should depend on other components of compositions, so that Osmolality maintains in preferred limit.By adding other energy metabolism products and cofactor can further improve these beverages.

The present invention will further illustrate by following examples.

embodiment 1 the impact that D-ribose is remedied nucleotide in static rat muscle

In theory but be not objectively to show, ribose synthesizes and has improved the synthesis rate through the ATP of nucleotide salvage by PRPP.Yet, for adenylic acid total in resting muscle (TAN) or ribose level without any understanding.Therefore may this synzyme approach by saturated, that the giving of ribose can't be improved is normal, without the ATP level of ischemia skeletal muscle.In order to prove the impact of ribose on this approach, the compound operation on muscle of healthy adult male Sprague-Dawley rat sole of the foot flesh is exposed and use the perfusion of reconstruct blood infusion medium, this medium contains bovine insulin/ml of aminoacid, mM glucose and 100 μ U.Muscle with perfusion in about 40ml/ minute, provides the perfused tissue of about 0.65ml/ minute by the reconstruct blood media.The D-ribose of variable concentrations is joined in infusion liquid so that its concentration reaches 0.156mM to 0.5mM, 1.58mM, 5.0mM and 15.0mM.Perfusion muscle 30 minutes.For 2 rats of the minimum analysis of each ribose test dose.

After perfusion, cut the muscle section and freeze folder with the aluminum pincers in liquid nitrogen by limbs rapidly.By muscle cut into slices lyophilization and in distilled water reconstruct for by anti-phase high pressure liquid chromatography, separating adenylic acid.The nanomole number (nM/gm/hr) of the adenine that result is per hour remedied with every gram muscle weight in wet base (that is, the formation of ATP) means.

Table I

Ribose skeletal muscle dose-response kinetics

Ribose	Observed value	Saturation kinetics by substrate
			0.000	48.6
0.158	113.0	85.82
			0.500	110.0	118.68
1.000		154.12
			1.580	188.5	183.51
2.000		199.74
			2.500		215.29
3.000		227.85
			5.000	250.0	260.68
15.000	315.5	310.37

As shown in Fig. 1 and Table I, during 0 mM of (mM) ribose, adenine is remedied lower than 50nM/gm/hr.When giving 0.158mM ribose, double.When 5mM ribose, the ATP synthesis rate reaches 250nM/gm/hr.These results show that normal, healthy muscle has low ribose baseline values and nucleotide is remedied ability, and these can improve by giving ribose.

embodiment 2 improve motor capacity in normal experimenter

Test 4 ages at 24-26 the healthy suitable test person between year.This group personnel's that select health level, sex and mean age are consistent, and there is no known metabolism, neuron, endocrine or heart and lung diseases.They all can or have experience by bike.Research approach comprises four-stage: (1) is by the initial baseline period formed without moving period; (2) load stage, give 3 D-riboses or placebo (glucose) totally 3 days every day; Within (3) 3 days, adopt the training stage of following moving period, it is characterized in that running at full speed with short time (10 seconds) the high strength circulation of the continuous bout of the resistance of 7% body weight (N=6), between running at full speed, have a rest 50 seconds, twice of every day (early morning and afternoon), and (4) are from last recovery stage of 48 hours after training period.Fig. 1 is a diagram that circulation is run back and closed at full speed.

Vastus lateralis (vastis lateralis muscle) to shank carries out muscle biopsy (MB) in order to be uniformly distributed and make living tissue sampling and possible myalgia minimum at every lower limb.Carry out MB first under static and carry out at once MB when research starts to set up baseline after the training period first in the 0th day or the 1st stage.At load stage, do not carry out MB.Carry out muscle biopsy after the last training and after recovering 48 hours.

Placebo or ribose group comprise two experimenters of random selection.In 3 days during training (load stage) 3 days before and training (training stage), isosmotic solution oral administration every day that ribose or glucose contain 10g ribose or placebo with 250ml 3 times.Give at once after activity that 0.5L etc. oozes electrolyte solution and after 30 minutes again to being avoided dehydration.

Measure the concentration of the following analyte in the MB sample: ATP, ADP, AMP, IMP (inosine monophosphate), TAN (total adenylic acid), creatine phosphate and creatine.

Table II

The research of athlete's ribose

Mean power/kilogram (watt)

Table III

The research of athlete's ribose

Power peak/kilogram (watt)

Table IV

The research of athlete's ribose

General power/kilogram

As Table II, to as shown in IV and Fig. 2 and 3, the behavior performance that makes of ribose improves 9%.

The raising of behavior performance is reflected in the ATP level in muscle biopsy.As shown in Table V, in the training stage, start to have higher ATP level with ribose preload experimenter in 3 days, reduce more obviously than the ATP level of placebo group running back at full speed after closing, it is more effective that this shows that ATP utilizes.With 78% of placebo group, compare, the ribose group returned to 82% of base level after 48 hours.

Table V

Average A TP value (mmol/kg dw)

Group	Before	Afterwards	Recovery rate	The recovery rate % of administration before	Front and back change	After administration-variation that recovers
							Placebo	23.60	20.05	18.30	78％	-3.55	-1.75
Ribose	25.33	13.90	20.80	82％	-11.43	6.90

embodiment 3 normal, without the training experimenter in the enhancing of energy and comfort

Before activity and during at once the administration D-ribose can improve beneficial effect for those experimenters that do not exercised.As described in above-mentioned embodiment 2, test 4 health, the normal male volunteer full speed race output in the exoergic circulation.Each experimenter serves as the contrast of himself.Between running back at full speed and closing, the experimenter should be gone in ring at a slow speed and continuously.Total testing time is 1 hour, this duration of test totally 4 full speed run back and close.After initial baseline determination and after each is run back at full speed and closes, the 5g D-ribose of using to this experimenter to be present in 200ml or the placebo (glucose) of similar taste.Measure 15 minutes the time after picked-up testing liquid and run at full speed output.Each experimenter experiences two periods with random order, and give ribose and give placebo a period period, and interval is one week therebetween.Placebo with the glucose dulcification in order to can't distinguish over ribose solution.Described experimenter gives ribose and shows than high output after using the placebo administration after continuing to relax motion.Can further think that these experimenters are subjective has a stronger comfort.

4 pairs of kinetic anginal alleviations of embodiment

Suffer from chronic arterial disease medical history, after three bypass operation of coronary artery, 68 years old male patient of state experiences kinetic angina pectoris.Its current medication is: enalapril (a kind of angiotensin-convertion enzyme inhibitor), carvedilol (Beta receptor blockers), Deponit TTS and the nitroglycerin Sublingual tablet used in case of necessity.It is fully closed that his coronary artery disease that shows up-to-date coronary angiogram develops into a bypass graft.This patient shows bad in two stress test.Its motion only limits to walking every day.

Because angor worsens, this patient can only walking be less than 1 mile every day, must Sublingual picked-up nitroglycerin after place of arrival.Oral administration gives this patient and is dissolved in the D-ribose in about 250cc water.In 6 months, this patient accepts to be interrupted the 5-10g D-ribose of dosage every day.After the ribose administration, this patient can improve its exercise tolerance every day and reach 2 miles, and without any need for the oral nitric acid administration supplemented.When interrupting ribose, it occurs the exercise induced angina pectoris before the ribose administration again, must use supplementary oral nitroglycerin.Again oral ribose makes this patient can walking every day 2 miles, angina pectoris does not occur or need nitroglycerin.Its subjective assessment to ribose to treat is " very weak angina pectoris, I feel better energy is more arranged and can carry out more multi-activity and no pain or medicine (nitroglycerin) ".

the improvement of 5 pairs of bicycle ergometor exercise behaviors of embodiment

The obturation of observing its more than epicardial coronary arteries by 1 60 years old male patient who suffers from lasting coronary artery disease surpasses 50 percent and lasting angor.Test this patient's treadmill behavior.After two baseline bicycle ergometor exercises, according to the Bruce scheme, he accepts D-ribose administration (40gm, 3 administrations of every natural gift) three days and completes bicycle ergometor exercise the 3rd time.At every turn, when appearring in the patient, following situation stops testing a) the ST section decline 1mm or when more in electrocardiogram (ECG) spike as the patient; B) when the patient complains angina pectoris or c) when the patient stops because of dyspnea or fatigue.In each test, this patient finishes test because of short of breath but without angina pectoris the time.

By Table VI, can be found out, by each stage of experiment with measuring, the speed while comprising static (0 constantly)-pressure result, give D-ribose in 3 days before the last bicycle ergometor exercise and can improve energy and cardiac function.General acceptable, the result of Heartbeat rate and whole body pressure is measuring of myocardial function and energy level, and the lower expression myocardial function of numerical value is better.The result of ribose administration, to the average tolerance time prolongation of treadmill.Except objective determination usefulness, the subjective proposition of patient feels more to have energy during the ribose administration.

Table VI

Heart beating number of times per minute is multiplied by the speed of systolic blood pressure mmHg-pressure result

Time	Baseline 1	Baseline 2	On average	Test	% changes
						0 (static) 3 minutes 6 minutes 9 minutes	11,08817,57426,50033,396	9,27213,46822,34429,526	10,18015,52124,42231,461	9,17715,27220,59225,356	-9.55％-1.60％-15.68％-9.87％
Tolerance time (second)	483.00	545.00	514.00	540.00	5.06％

In the Bruce scheme, treadmill speed was increased to 6.0 miles per hours by 1.7 miles per hours in 3 minutes, and slope increases to 22% by 10% simultaneously.

the self-administration of embodiment 6 ribose

Suffer from chronic disease (including but not limited to coronary artery disease, AIDS, intermittent claudication, tuberculosis and chronic fatigue syndrome) and it is characterized in that the patient that energy level is low, and those there is no obvious disease but the low experimenter of energy level due to restoring because of aging, wound, burn with by ill or operation can be without being benefited by improving its energy level when continuing medical intervention.Many individualities with relatively stable disease pass through in accordance with a kind of altered life style, and supplementing of bound drug second after second survived.Usually, these experimenters are prevented from the physical exertion of subordinate appropriateness because worrying to cause sense of discomfort as angina pectoris, breathless, myalgia, cramp or sense tired out.This escape has reduced these experimenters' quality of life and has produced the background anxiety existed all the time.In addition, these experimenters have been deprived of useful or appropriate motion, comprise and strengthen digestion, sleep and the more lax and positive mental status.Even without disease but the experimenter of health worry may be subjective dissatisfied to its energy level and comfort.

The example that there is no obvious disease but have benefited from the experimenter of ribose self-administration is 55 years old old man.He adheres to weekly strict motion scheme in most of the time until continue the system antibacterial and infect in its life, need to stay in the intensive care chamber 1 month and rehabilitation 1 month again.Its cardiovascular and pulmonary system mainly its during one's sickness and afterwards infected and 1 year after function can't return to previous level, or make him please oneself.

After convalescence, he attempts to recover motion scheme, comprises run on treadmill weekly 4 days and weight lifting in 2 days weekly.But run restriction at short notice.After motion every day, he continues to feel that fatigue reaches the collapse point and need to often take a nap.The patient starts oral D-ribose voluntarily, gives 2 dosage every day, each dosage 4-5g.In 7 days, he claims that its " vigor " and exercise tolerance strengthen to some extent.Can on treadmill, run and reach 30 minutes first after he is ill.He still feels fatigue to a certain degree, but can after motion, stop nap.He continues oral ribose administration every day, adheres to planned motion simultaneously, and feels after 4 weeks that in the ribose administration its energy level continues to improve.Ribose has no side effect to him.

embodiment 7 ribose and arginine and/or interior alkali are on suffering from chronic disease experimenter's impact

As described in Example 6, can foretell that the experimenter of experience low energy level can benefit from the self-administration of pentose.Can further infer that the oral acceptable vasodilation of picked-up will have additional advantageous effects to this type of experimenter as L-arginine.Still can further foretell that the picked-up VBT be take and transport fatty acid and enter mitochondrion and will provide other beneficial effect as these experimenters.Can also estimate to add other energy metabolism products and cofactor to provide additional beneficial effect for this type of experimenter.

Known arginine is the nitric oxide production precursor of endothelium relaxation factor.Analyzed in vitro is under normal circumstances verified, and endotheliocyte can obtain excessive L-arginine.Yet in vitro study also shows, if when the storage of L-arginine is depleted or the L-glutamine, when a kind of antagonist of L-arginine exists, endothelium dependent form vasodilation can be improved by adding L-arginine.Unknown before the present invention, whether oral arginine can strengthen heart perfusion and make thus ribose be distributed in muscular tissue.Selected test group should be because of the low trouble people of heart disease energy level, and it is the colony that can utilize and fully study.Expected results can be applied to have other experimenters (experimenter and old people and the dog that for example suffer from weak disease) of low energy level comparably.

To suffer from known stable coronary artery disease but be divided at random 3 independently groups without static ischemic 30 (45-70 year) experimenters that grow up.Each experimenter should accept continuous bicycle ergometor exercise at first to confirm qualified this scheme that adds.Giving L-arginine, D-ribose, VBT or carrying out the last bicycle ergometor exercise after 3 days containing the compositions of L-arginine, D-ribose and VBT.The terminal of this research is investigation anginal progress and/or Electrocardiographic variation during treadmill movement.

Expect that these experimenters' improvement is larger than the speed-pressure decreased 10% described in embodiment 2 and tolerance time increase by 5%.

All documents and the full patent texts at this, quoted are incorporated herein by reference.The present invention has described a plurality of concrete and preferred embodiments.However, it should be understood that within the spirit and scope of the present invention and can carry out multiple variation or improvement.

Claims (4)

1. the purposes in the compositions that ribose improves motor capacity in for the preparation of the normal subjects and the rear energy that moves recovers, the D-ribose that wherein said compositions comprises the 0.1-100 gram.

2. the purposes of ribose in the compositions for the preparation of improving patient's myocardial function, the D-ribose that wherein said compositions comprises the 0.1-100 gram.

3. according to the purposes of claim 1 or 2, wherein said compositions comprises pharmaceutical excipient.

4. according to the purposes of claim 3, wherein said excipient is solid or semisolid edible excipient or liquid excipient.

Images