CN101253868A - Biological preparation for preventing and controlling pepper powdery mildew and preparation method and application thereof - Google Patents
Biological preparation for preventing and controlling pepper powdery mildew and preparation method and application thereof Download PDFInfo
- Publication number
- CN101253868A CN101253868A CNA2007100663885A CN200710066388A CN101253868A CN 101253868 A CN101253868 A CN 101253868A CN A2007100663885 A CNA2007100663885 A CN A2007100663885A CN 200710066388 A CN200710066388 A CN 200710066388A CN 101253868 A CN101253868 A CN 101253868A
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- CN
- China
- Prior art keywords
- preparation
- powdery mildew
- agent
- egg yolk
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 241000221785 Erysiphales Species 0.000 title claims abstract description 19
- 239000006002 Pepper Substances 0.000 title claims abstract description 17
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 17
- 235000017804 Piper guineense Nutrition 0.000 title claims abstract description 17
- 235000008184 Piper nigrum Nutrition 0.000 title claims abstract description 17
- 244000203593 Piper nigrum Species 0.000 title 1
- 241000722363 Piper Species 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 12
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 9
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 9
- 239000008157 edible vegetable oil Substances 0.000 claims abstract description 9
- 235000013345 egg yolk Nutrition 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 239000001390 capsicum minimum Substances 0.000 claims description 9
- 240000008574 Capsicum frutescens Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000000575 pesticide Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 5
- 230000002265 prevention Effects 0.000 abstract 2
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 abstract 1
- 239000003124 biologic agent Substances 0.000 abstract 1
- 231100000956 nontoxicity Toxicity 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 241000208293 Capsicum Species 0.000 description 7
- 230000000853 biopesticidal effect Effects 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 150000003851 azoles Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 208000006558 Dental Calculus Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000896221 Leveillula taurica Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 231100000584 environmental toxicity Toxicity 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Seasonings (AREA)
Abstract
The invention relates to a biological agent for preventing the powdery mildew in pepper and the preparation method and the application thereof. The invention belongs to the technology field of biological pesticides. The agent is composed of raw egg yolk 19.5g, edible oil 1L and water 15kg. The preparation method includes: (a) taking the raw egg yolk 19.5g out and rinsing with the clear water for further use; (b) blending the egg yolk by the juice extractor, adding the edible oil 1L and blending the mixed liquid until the liquid is milk white; (c) adding the agent prepared in the method to the water 15kg and fully stirring the agent evenly. The prevention and cure effect can be achieved at more than 65% by spraying the agent on the pepper with the powdery mildew for three times. The agent has low-cost material, easy manufacture technology, good prevention effect, non-toxicity, harmlessness, non-environment pollution and good application prospect.
Description
Technical field:
The invention belongs to biological pesticide technical field, be specifically related to a kind of biologic product of preventing and treating pepper powdery mildew and its production and application.
Background technology:
Pepper powdery mildew is to be caused by Tartar's internal thread powdery mildew (Leveillula taurica (Lev.) Arn.), belongs to sac fungi, Erysiphales; No condition is that capsicum is intended the powder spore, belongs to imperfect fungus, hyphomycetales.This disease is a capsicum middle and later periods disease, has popular characteristics rapidly, and under the suitable situation of environmental condition, short-term can be able to be very popular, and is one of disease important during capsicum produces.
For a long time, pepper powdery mildew mainly relies on the chemical agent control, as triadimefon, carbendazim, sulphur colloidal suspension agent etc., cause disease to develop immunity to drugs, threaten the person poultry safety simultaneously, cause the increase of residue of pesticide, natural enemy is killed and wounded in a large number, microecological balance destroyed and environmental pollution in farmland does not meet the requirement that pollution-free vegetable produces.
Along with China joined WTO and people attention day by day to environmental and ecological protection; biological control is pollution-free with it; noresidue, no eco-toxicity and safety are good etc., and advantage plays leading role in the control of plant pest, greatly develops biopesticide China is had very important realistic meaning.Biopesticide is the important source of novel pesticide initiative, plays important effect in the biological control of plant pathogenic fungi disease.Biopesticide is easy to decompose to the person poultry safety,, is described as " nuisanceless " agricultural chemicals with environmentally compatible.Since the nineties, biopesticide has become one of focus of researching and developing in the agricultural biotechnologies industry from eighties of last century.
Summary of the invention:
The objective of the invention is provides a kind of biologic product of preventing and treating pepper powdery mildew and its production and application in order to address the above problem.
The present invention screens a kind of biologic product that pepper powdery mildew is had fine preventive and therapeutic effect from a plurality of prescriptions.(biologic product called after EGY of the present invention.) in existing bibliographical information, do not see to have and adopt egg yolk to be used for disease control with the oily prescription that mixes, particularly to the control of pepper powdery mildew.
Technical scheme provided by the invention is:
1, a kind of biologic product of preventing and treating pepper powdery mildew, form by following content and component:
Raw egg yolk 19.5g,
Edible oil 1L,
Water 15kg
2, the method for the above-mentioned preparation of preparation:
(1) prepares before the preparation; 1 of raw egg, edible oil, juice extractor.
(2) preparation process:
(a) take out raw egg yolk 19.5g, use the clear water rinsing, stand-by;
(b) with above-mentioned yolk juice extractor mixing, add the 1L edible oil, mixing to mixed liquor is a milky;
(c) preparation that method for preparing is obtained joins in the 15Kg water, stirs.
Above-mentioned steps is all carried out under normal temperature and normal pressure.
3, the application of above-mentioned preparation: with the preparation that method for preparing obtains, evenly be sprayed at the front and the back side of capsicum blade, spray area is 200m
2
Field control effectiveness test is the result show: the biologic product for preparing with said method to the control efficiency of pepper powdery mildew more than 65%.
Utilize the control to pepper powdery mildew of biologic product that the present invention produces, it is good to have control efficiency, nontoxic, harmless environmental protection, and cost of material is low, and the advantage that production technology is simple is easy to industrialization and commercialized development, has application promise in clinical practice.
Embodiment:
1, small plot experiment:
Totally 3 processing are respectively biologic product of the present invention (handling 1), positive control (handling 2), blank (handling 3), and every processing is established 3 and repeated, and each sub-district area is about 20m
2, test gross area 180m
2
Positive control: the pure and strong milk liquid of 5% own azoles (SC).
2, prepare used biologic product:
(1) prepares before the preparation; 1 of raw egg, edible oil, juice extractor.
(2) preparation process:
(a) take out raw egg yolk 19.5g, use the clear water rinsing, stand-by;
(b) with above-mentioned yolk juice extractor mixing, add the 1L edible oil, mixing to mixed liquor is a milky;
(c) preparation that method for preparing is obtained joins in the 15Kg water, stirs.
Above-mentioned steps is all carried out under normal temperature and normal pressure.
3, medicament usage amount:
(1) handle 1 (join biologic product): the above-mentioned biologic product that preparation is obtained get its 1/3, evenly be sprayed at 60m
2Capsicum face of blade and the back side (pepper powdery mildew occurs in vacuum side of blade, so the capsicum back side will be sprayed evenly).
(2) handle 2 (positive controls): with the pure and strong milk liquid of the own azoles of 3ml5%, join in the 4.5Kg water, stir, evenly be sprayed at 60m
2The capsicum face of blade and the back side.
(3) handle 3 (blanks):, evenly be sprayed at 60m with equivalent clear water 4.5Kg
2The capsicum face of blade and the back side.
4, investigation method:
Every dispenser in 7 days 1 time, 7 days investigation disease indexs after 3 dispensers.A situation arises for every duplication 10 strain pepper powdery mildews.
5, grade scale:
0 grade: the whole anosis leaf of strain;
1 grade: the sick leaf rate of whole strain is below 5%;
3 grades: the sick leaf rate of whole strain is at 6%-10%;
5 grades: the sick leaf rate of whole strain is at 11%-20%;
7 grades: the sick leaf rate of whole strain is at 21%-50%;
9 grades: the sick leaf rate of whole strain is more than 51%.
6, control efficiency computational methods:
Disease index=[(∑ (the sick level number of sheets * represent progression))/(the number of sheets sum * the highest grade value of representing)] * 100
Control efficiency (%)=[(control group disease index-processed group disease index)/control group disease index] * 100%
7, experimental result:
| Handle | 3 are repeated average disease index | 3 are repeated average control efficiency (%) |
| Preparation is handled | 5.93 | 65.20 |
| Positive control | 7.32 | 57.04 |
| Blank | 17.04 | - |
Claims (6)
1, a kind of biologic product of preventing and treating pepper powdery mildew, form by following content and component:
Raw egg yolk 19.5g,
Edible oil 1L,
Water 15kg.
2, the preparation method of the described biologic product of claim 1 is characterized in that: this method may further comprise the steps:
(a) take out raw egg yolk 19.5g, use the clear water rinsing, stand-by;
(b) with above-mentioned yolk juice extractor mixing, add the 1L edible oil, mixing to mixed liquor is a milky;
(c) preparation that method for preparing is obtained joins in the 15kg water, stirs;
Above-mentioned steps is all carried out under normal temperature and normal pressure.
3, the application of the prepared biologic product of described biologic product of claim 1 or claim 2 is characterized in that: be used to prevent and treat pepper powdery mildew.
4, the described application of claim 3 is characterized in that: it is 200m that the preparation of this dosage is used area
2
5, the described application of claim 3 is characterized in that: executed said preparation 1 time every 7 days, execute altogether 3 times.
6, the described application of claim 3 is characterized in that: the front and the back side that said preparation evenly are sprayed at the capsicum blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100663885A CN101253868A (en) | 2007-11-20 | 2007-11-20 | Biological preparation for preventing and controlling pepper powdery mildew and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100663885A CN101253868A (en) | 2007-11-20 | 2007-11-20 | Biological preparation for preventing and controlling pepper powdery mildew and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101253868A true CN101253868A (en) | 2008-09-03 |
Family
ID=39889258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100663885A Pending CN101253868A (en) | 2007-11-20 | 2007-11-20 | Biological preparation for preventing and controlling pepper powdery mildew and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101253868A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068067A (en) * | 2014-06-20 | 2014-10-01 | 浙江省农业科学院 | Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests |
| CN107306946A (en) * | 2017-05-10 | 2017-11-03 | 中国农业大学 | A kind of egg oil agricultural chemicals and its preparation method and application |
-
2007
- 2007-11-20 CN CNA2007100663885A patent/CN101253868A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068067A (en) * | 2014-06-20 | 2014-10-01 | 浙江省农业科学院 | Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests |
| CN104068067B (en) * | 2014-06-20 | 2017-01-25 | 浙江省农业科学院 | Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests |
| CN107306946A (en) * | 2017-05-10 | 2017-11-03 | 中国农业大学 | A kind of egg oil agricultural chemicals and its preparation method and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080903 |