CN101188069B - Device for simulating space gravity biological test in high grads strong magnetic field - Google Patents
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Abstract
本发明涉及一种大梯度强磁场中进行空间重力生物学模拟实验的装置，超导磁体固定在水平地面上，控温水胆垂直插入超导磁体中间的圆柱形空腔中。载物升降装置底座位于超导磁体的底部，载物升降装置的托盘插入带有控温水胆的超导磁体空腔中。参数监测装置安装在载物升降装置托盘的底部。载物升降装置由导线连接到数据显示装置上。超导磁体的空腔上下两端分别被气体控制装置的密封塞密封。CCD摄像位于环状照明光纤中心，二者同步伸入空腔中，置于托盘上方。本发明提供的装置可以实现温度、湿度、试样位置、重力水平、磁场强度等综合指标的精确测量和控制，能够满足从分子、细胞、组织、器官到整体层次进行空间生命科学研究的模拟实验条件需要。
The invention relates to a device for carrying out space gravity biological simulation experiments in a large gradient strong magnetic field. A superconducting magnet is fixed on a horizontal ground, and a temperature-controlling water tank is vertically inserted into a cylindrical cavity in the middle of the superconducting magnet. The base of the load lifting device is located at the bottom of the superconducting magnet, and the tray of the load lifting device is inserted into the cavity of the superconducting magnet with a temperature-controlled water bladder. The parameter monitoring device is installed at the bottom of the pallet of the load lifting device. The load lifting device is connected to the data display device by wires. The upper and lower ends of the cavity of the superconducting magnet are respectively sealed by sealing plugs of the gas control device. The CCD camera is located in the center of the ring-shaped lighting fiber, and the two are synchronously extended into the cavity and placed above the tray. The device provided by the invention can realize accurate measurement and control of comprehensive indicators such as temperature, humidity, sample position, gravity level, magnetic field strength, etc., and can meet the simulation experiments of space life science research from molecules, cells, tissues, organs to the whole level Conditions require.
Description
Technical field
The present invention relates to the device that carries out the space gravitational biology simulated experiment in a kind of big strong magnetic field gradient, in space science, comprise in the ambits such as spatial cell biology, space developmental biology, space structure biology, space microbiology and space material a kind of experimental installation is provided.
Background technology
For a long time, space life science research generally needs to simulate and test under the ground experiment condition in advance, i.e. ground experiment according to the ground experimental result, is adjusted design space experiment parameter and condition, carries out the space experimental comparison and the checking of exploration again.The reliability of ground preliminary experiment is depended in the success or not of space experiment to a great extent.Yet because the normal gravity environment of space gravity environment and ground is very different, the experiment parameter that the ground preliminary experiment under the normal gravity condition obtains is difficult to guarantee the success of space experiment sometimes.Success ratio for the utilising efficiency that improves SPACE MICROGRAVITY RESOURCE and space experiment, be necessary to set up the ground preliminary experiment platform of space experiment on ground, with as far as possible near the condition of space gravity, carrying out ground tests in advance, obtain suitable experiment parameter, as the reference of formulating the space experiment parameter.
Carry out weightless flight on ground, to realize satisfying the lasting weightlessness of long period of life science needs.Ground experimental technique in the past realizes 10 seconds weightlessness usually at most, and for most of scientific researches, such condition is far from being enough.In recent years, along with the superconducting magnet technology rapid development, for the simulation of the ground of space below-G conditions provides a new solution route.The superconducting magnet of particular design can produce powerful stable gradient magnetic and (can reach-1500T
2/ m), utilize material in this gradient magnetic to be subjected to the different changes that realize material gravity state in magnetic field of big or small direction of magnetizing force.The superconducting magnet that possesses strong magnetic gravitational effect, its most outstanding advantage are longer duration (can reach more than 4 years).In addition, a superconducting magnet has characteristics overweight and weightless zone simultaneously, make Gravity changer become very easy: only to need place magneticstrength identical but the inconsistent zone of gravity compares experiment experimental subjects, can obtain the influence of gravity to experimental result to the influence of experimental subjects research.Estimate that these advantages will make the application of strong magnetic gravitational effect become one of space life science research field important advanced research direction.At present abroad, the support of some great research projects (as Japanese Protein 3000) has begun to show this trend.
U.S. Brown university Valles laboratory is (NAG 8-1782 under Nasa (NASA) supports, NAG 8-1774 and NNA 04CC57G), take the lead in having carried out magnetic levitation Mars and lunar gravity simulation analysis and applied research thereof, and obtained preliminary result of study.Studies show that magnetic levitation Mars and lunar gravity simulator have influenced the spilting of an egg of frog embryos, but the growth of frog is not had the influence of lethality.Units such as Japan Tsukuba high-intensity magnetic field research centre (Japanese material research institution), Dutch Nijmegen high-intensity magnetic field center and U.S. Tallahasse high-intensity magnetic field research centre have all carried out the Life Science Experiment research under strong magnetic simulated gravity environment, and have obtained preliminary result of study.
The space gravity effect that present superconducting magnet is produced is mainly used in area researches such as materialogy, physics, does not still have the strong magnetic gravitational biology technology platform specific equipment that is directly used in life science.
Summary of the invention
The technical problem that solves
For fear of the deficiencies in the prior art part, the present invention proposes the device that carries out the space gravitational biology simulated experiment in a kind of big strong magnetic field gradient, be intended to set up a kind of new space gravity environment ground simulated experiment dress isolated plant and technology, promptly utilize strong magnetic gravitational effect to carry out the simulation of space gravity environment ground, in the superconducting magnet room temperature chamber, manufacture and design the device that satisfies space life science ground experiment usefulness, the space life science ground experimental study technology that exploitation is relevant therewith.
Technical scheme
Technical characterictic of the present invention is: comprise superconducting magnet 1, loading lifting device 2, control warm water liner 3, parameter monitor device 4, loading lifting device base 5, watch-dog 6, lighting fiber 7, subminiature CCD camera 8, lead 9, data presentation device 10 and gas control equipment 11.Superconducting magnet 1 is fixed on the level ground, control warm water liner 3 vertical insertions in the superconducting magnet 1 intermediary cylindrical cavity.Loading lifting device base 2 is positioned at the below of superconducting magnet 1, and the pallet of loading lifting device 2 inserts and has in superconducting magnet 1 cavity of controlling warm water liner 3.Parameter monitor device 4 is installed in the bottom of loading lifting device 2 pallets.Loading lifting device 2 is connected on the data presentation device 10 by lead 9.The cavity of superconducting magnet 1 is sealed by the sealing plug of gas control equipment 11 respectively at two ends up and down.Subminiature CCD camera 8 is positioned at ring-shaped lighting optical fiber 7 centers, and the two stretches in the cavity from superconducting magnet 1 cavity top synchronously, places the pallet top.
Described loading lifting device 2 comprises pallet 13, pillar 14, guide deflection sheave 15, winch 16, pulley 17, guide rail 18, base 19, chassis 20, counterweight 21, rope 22.Pallet 13 is fixedly connected on pillar 14 upper ends, and pillar 14 is clipped between the guide deflection sheave 15, and the lower end is fixed on the chassis 20, and chassis 20 is between two row's guide rails 18, and rope 22 1 ends are fixed on the chassis 20, and the other end is walked around winch 16 and is connected counterweight 21 with pulley 17.
Described pallet 13 manufactured materials adopt 62 brass, red copper, aluminium alloy, plastics diamagnetic substance.
Described control warm water liner 3 comprises water-in 23, water outlet 24, upper cover 25, outer wall 26, inwall 27, parting bead I28, parting bead II29, lower cover 30.Become interlayer by outer wall 26 with inwall 27 two-layer thin-walled round shapes, the thin cylinder upper/lower terminal has upper cover 25 and lower cover 30, and interlayer is thick to be 2mm.The centre has spiral parting bead I 28 and parting bead II 29 that interlayer is divided into two spiral channels.Article one, be descending water inlet spiral channel, one is up water outlet spiral channel.Article two, spiral channel is communicated with in the bottom.Water-in 23 water outlets 24 are all in the upper end.Water-in 23 is positioned at descending spiral channel inlet, and water outlet 24 is positioned at the import of upstream screw water channel.
Described control warm water liner 3 manufactured materials can be 62 brass, red copper, aluminium alloy, plastics diamagnetic substance.
Described parameter monitor device 4 comprises temperature sensor 31, gravity sensor 32, displacement sensor 33 and sensor lead 34.Temperature sensor 31, gravity sensor 32 and displacement sensor 33 are configured in pallet 13 belows, and output transducer lead-in wire 34 is embedded in the pillar 14, and draw through lead 9 along pillar 14 and to be connected on the data presentation device 10.
Described temperature sensor 31 is selected 0.1 grade of type pt100 for use.
Described gravity sensor 32 is selected twin beams shearing-force type structure for use, the material berylliumbronze.
Described gas control equipment 11 comprises conduit 35, air storage chamber 36, inlet mouth I 37, air pump 38, inlet mouth II 39, lower seal plug 40, goes up sealing plug 41 and air outlet 42.Two ends are by last sealing plug 41 and 40 sealings of lower seal plug up and down for superconducting magnet 1 cavity, and air outlet 42 and inlet mouth II 39 go up on sealing plug 41 and the lower seal plug 40 position respectively.Inlet mouth II 39 and air outlet 42 are connected on the air pump by conduit respectively, and air pump is connected on the air storage chamber 36 by conduit 35 through inlet mouth I 37.
Beneficial effect
The present invention is on the basis of introducing the nucleus equipment superconducting magnet, research and develop the supporting with it various supporting special experimental installation that is applicable to space life science research, made up the ground simulated experiment technical qualification of the space life science research of one " long-time, high stable gravity environment ".Device provided by the invention can be realized the accurate measurement and the control of overall targets such as temperature, humidity, sample position, gravity level, magneticstrength, can satisfy the simulated experiment condition needs that carry out space life science research from molecule, cell, tissue, organ to whole level fully.
Utilize this device, can carry out relevant space biology effect and space biotechnology research.By this simulated experiment, compare with the space experiment, checking is at the simulate effect of each subdiscipline of space life science field, and how research provides suitable experimental technique, experiment parameter and experimental subjects for finally carrying out the satellite lift-launch or carrying out correlative study in the space station, to improve specific aim, reliability and the success ratio of space life science research, ensure carrying out smoothly of space life experiment.
Description of drawings
Fig. 1: structural representation of the present invention
Fig. 2: loading lifting device structure synoptic diagram
Fig. 3: the control warm water liner shows the structure intention
Fig. 4: parameter monitor device structural representation
Fig. 5: gas control equipment structural representation
1. superconducting magnet; 2. loading lifting device; 3. control warm water liner; 4. parameter monitor device; 5. loading lifting device base; 6. watch-dog; 7. lighting fiber; 8. subminiature CCD camera; 9. lead; 10. data presentation device; 11. gas control equipment; 12. tried object; 13. pallet; 14. pillar; 15. guide deflection sheave; 16. winch; 17. pulley; 18. guide rail; 19. base; 20. chassis; 21. counterweight; 22. rope; 23. water-in; 24. water outlet; 25. upper cover; 26. outer wall; 27. inwall; 28. parting bead I; 29. parting bead II; 30. lower cover; 31. temperature sensor; 32. gravity sensor; 33. displacement sensor; 34. sensor lead; 35. conduit, 36. air storage chambers, 37. inlet mouth I, 38. air pumps, 39. inlet mouth II, 40. lower seal plugs, sealing plug on 41., 42. air outlets.
Embodiment
Now in conjunction with the accompanying drawings the present invention is further described:
Can not be in order to overcome the prior art existence to the accurate shortcoming of measuring and controlling of overall targets such as biological sample present position temperature, humidity, gas, gravity level, magneticstrength, and can not the variation of Real Time Observation biological sample etc. the deficiency of aspect, the invention provides a kind of device that in big strong magnetic field gradient, carries out the space gravitational biology simulated experiment.
This device (as shown in Figure 1) comprises superconducting magnet 1, loading lifting device 2, control warm water liner 3, gas control equipment 11, parameter monitor device 4, data presentation device 10, loading lifting device base 19, watch-dog 6, lighting fiber 7 and subminiature CCD camera 8.Superconducting magnet 1 is fixed on the level ground, in the middle of the superconducting magnet cylindrical cavity is arranged, 50 millimeters of diameters, cylindric control warm water liner 3 vertical insertions in the cavity.Loading lifting device base 19 is positioned at superconducting magnet 1 bottom, and the pallet 13 on the loading lifting device 2 can move up and down, and pallet 13 can enter and have in the superconducting magnet cavity of controlling warm water liner 3; Superconducting magnet cavity two ends up and down makes cavity become the part of gas control equipment 11 respectively by the sealing of the sealing plug of gas control equipment 11.Parameter monitor device 4 is installed in the bottom of loading lifting device 2 pallets 13 respectively; Subminiature CCD camera 8 stretches in the cavity from superconducting magnet cavity top, places pallet sample top, provides illumination by lighting fiber 7, and live signal is transferred into watch-dog 6, is convenient to Real Time Observation sample situation.
Can be the cylindrical cavity of Φ 50mm * 450mm with the space for experiment in the superconducting magnet 1, the big strong magnetic field gradient that the cavity inner magnet produces acts on the diamagnetism laboratory sample, and the magnetizing force of generation can be offset gravity partially or completely and be produced weightlessness; Or reinforcement gravity produces genvironments.Three special apparent gravity positions that can be used for testing are arranged: 2g, 1g and 0g in the cavity.With loading lifting device 2 laboratory sample is sent to above-mentioned specific position or other position in the cavity, sample is under the different gravity environments.
Loading lifting device 2 (as shown in Figure 2) is by pallet 13, pillar 14, and guide deflection sheave 15, winch 16, pulley 17, guide rail 18, base 19, chassis 20, counterweight 21, rope 22 is formed.Pallet 13 is fixedly connected on pillar 14 upper ends, and pillar 14 is clipped in the middle of the guide deflection sheave 15, keeps vertical direction, and the lower end is fixed on the chassis 20, and chassis 20 is between two row's guide rails 18, can slide up and down along guide rail 18.Rope 22 1 ends are fixed on the chassis 20, and the other end is walked around winch 16, pulley 17 is connected with counterweight 21.Elevating function is finished by winch 16, and the rope on the winch 16 is used for parts such as lifting tray 13, guide deflection sheave 15, pulley 17, guide rail 18 and guarantees pallet 13 horizontal vertical liftings.
In order to realize that research object accuracy control over temperature (4-60 ℃) selected for use the water-bath temperature control method.Adopt temperature sensor 31 to obtain the sample actual temperature, and temperature is controlled by regulating water-bath.In order to guarantee less temperature fluctuation, also need room temperature is controlled, and the water-bath pipeline is implemented the insulation measure.The realization of insulation measure: control warm water liner 3 is housed in superconducting magnet 1.During experiment, the control warm water liner 3 magnet cavity, fixing of packing into, sample is sent in control warm water liner 3 cavitys by loading lifting device 2, makes sample be in different experiment positions.
The structure of control warm water liner 3 is made up of water-in 23, water outlet 24, upper cover 25, outer wall 26, inwall 27, parting bead I28, parting bead II29, lower cover 30 as shown in Figure 3.Inwall 27 and outer wall 26 are formed cylindric interlayer, form enclosed space with upper cover 25 and lower cover 30 respectively again.Water-in 23 and water outlet 24 are positioned at control warm water liner top, and parting bead I28, parting bead II29 spirrillum are in cylindric interlayer.Temperature control water 3 courages are contained in superconducting magnet 1 inside, and the cylindrical cavity that inwall forms is used for placing sample.Interlayer is thick to be 2mm, two spiral channels that the centre has Double-spiral parting bead 28,29 that the water courage is divided into, one is descending water inlet spiral channel, one is up water outlet spiral channel, article two, spiral channel is communicated with in water courage bottom, and intake-outlet is all in the upper end of controlling warm water liner 3.Hot water is flowed into through descending spiral channel by water-in 23 and flow to control warm water liner 3 lower ends, turns back to water outlet 24 by the upstream screw water channel again and flows out, and hot water flow is had living space through the institute of control warm water liner interlayer like this, makes the cavity temperature of placing sample even.Consider operational characteristic, the control warm water liner is selected the processing of 62 brass materials for use.During experiment, the control warm water liner 3 superconducting magnet cavity 1, fixing of packing into, sample is sent in the control warm water liner cavity by loading lifting device 2, makes sample be in the different experiments position.
Parameter monitor device 4 is made up of temperature sensor 31, gravity sensor 32, displacement sensor 33, sensor lead 34 as shown in Figure 4.Gravity sensor 32 and displacement sensor position 33 are in the tray bottom, and temperature sensor 31 is fixed on around the pallet 13, and output transducer lead-in wire 34 is embedded in the pillar 14, and is descending along pillar 14.Three parameters of main monitoring: the displacement of gravity, temperature and laboratory sample.In order to realize the real-time measurement of Gravity changer and sample envrionment temperature of living in, below pallet 13, dispose gravity sensor 32 and temperature sensor 31.Output transducer lead-in wire 34 is drawn by pillar 14 and is received on the data presentation device 10.The range of gravity is 100 grams, and resolving power is 0.1 gram, and the temperature range is 100 ℃, and resolving power is 0.1 ℃.Tried object in the intravital accurate position of superconducting magnet magnetic for measuring, displacement sensor 32 is housed on the pallet, the electrical signal of its output is delivered to data presentation device by output transducer lead-in wire 34 and shows 10.This loading lifting device structure is simple, reliable, and displacement measurement is accurate, and range 400mm, displacement resolving power are 0.1mm.Under ultrastrong magnetic field (16T), measure parameters such as gravity, temperature, displacement and need consider the influence of high-intensity magnetic field sensor function.Our used gravity sensor 32 is selected twin beams shearing-force type structure for use, avoids the influence of origin of force difference to measured value; Select the little material of susceptibility, pallet 62 brass, gravity sensor 32 is used berylliumbronze; Temperature sensor 31 is selected 0.1 grade of type pt100 for use; Each sensor lead is drawn with twisted-pair feeder parallel flux lines direction, to avoid high-intensity magnetic field the interference of signal transmission is caused measuring error.
In order to realize that to Real Time Observation subminiature CCD camera 8 is positioned at ring-shaped lighting optical fiber 7 central authorities, both position relative fixed to laboratory sample.Subminiature CCD camera is placed control warm water liner cavity sample top, and lighting fiber 7 provides illumination functions, forms viewing system easily.Subminiature CCD camera 8 carries out IMAQ, and signal is sent to computer away from magnet through conversion, utilizes watch-dog 6 to realize the experimentation of research object is observed.
Gas control equipment 11 is made up of conduit 35, air storage chamber 36, inlet mouth I37, air pump 38, inlet mouth II39, lower seal plug 40, last sealing plug 41 and air outlet 42 as shown in Figure 5.Two ends are by last sealing plug 41 and 40 sealings of lower seal plug up and down for superconducting magnet 1 cavity, and air outlet 42 and inlet mouth II39 position respectively go up on sealing plug 41 and the lower seal plug 40; Inlet mouth II39 and air outlet 42 are connected on the air pump 38 by conduit 35 respectively.Air pump 38 is connected on the air storage chamber 36 by conduit 35 through inlet mouth I37.The experiment desired gas is stored in the air storage chamber 36 in advance, when gas is not enough in the cavity, by air storage chamber 36 make-up gas, realizes the real-time control to biological sample growth desired gas.
In order to realize to biological sample (cell) growth desired gas (95% oxygen, 5% carbonic acid gas) control in real time, the mixed gas that will contain 95% oxygen, 5% carbonic acid gas is stored in the air storage chamber 36 in advance, delivers gas in superconducting magnet 1 airtight chamber by air pump 38.Concrete gas control embodiment: the superconducting magnet cavity is by last sealing plug 41 and 40 sealings of lower seal plug, and inlet mouth II39 and air outlet 42 are connected on the air pump 38 by conduit 35 respectively.Inlet mouth I37 is connected on the air storage chamber 36 by conduit 35.When gas is not enough in the cavity, by air storage chamber 36 make-up gas.
The culture dish that inoculation is had cell, be positioned on the loading lifting device 2, by loading lifting device 2 parameters and monitoring device 4 cell is positioned in superconducting magnet 1 chamber 3 gravity position: 0g, 1g and 2g, by control warm water liner 3 with temperature-stable in optimal temperature, carry out Real Time Observation by Real Time Observation system-watch-dog 6, lighting fiber 7 and subminiature CCD camera 8, by gas control equipment 11, required 95% oxygen, 5% carbonic acid gas of control cell growth.After cultivating certain hour, culture dish is taken out, detect the influence of strong magnetic gravity environment pair cell.
Claims (9)
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| CN103756899B (en) * | 2014-02-18 | 2016-03-09 | 中国科学院合肥物质科学研究院 | Can be used for biological cultured continuously and the microscopic imaging device of stable state high-intensity magnetic field effect study |
| CN112662543A (en) * | 2021-01-05 | 2021-04-16 | 中国人民解放军63919部队 | Multifunctional cell overweight effect experimental device |
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|---|---|---|---|---|
| US5104802A (en) * | 1989-07-28 | 1992-04-14 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Hollow fiber clinostat for simulating microgravity in cell culture |
| US5379657A (en) * | 1992-06-22 | 1995-01-10 | Timothy K. Hasselman | Microgravity suspension system for simulating a weightless environment |
| CN2334067Y (en) * | 1998-07-15 | 1999-08-18 | 李俊伦 | Microgravity experiment instrument |
| EP1500944A1 (en) * | 2003-06-27 | 2005-01-26 | Silvia Bradamante | A bioreactor, in particular for NMR spectroscopy |
-
2006
- 2006-11-15 CN CN 200610104905 patent/CN101188069B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5104802A (en) * | 1989-07-28 | 1992-04-14 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Hollow fiber clinostat for simulating microgravity in cell culture |
| US5379657A (en) * | 1992-06-22 | 1995-01-10 | Timothy K. Hasselman | Microgravity suspension system for simulating a weightless environment |
| CN2334067Y (en) * | 1998-07-15 | 1999-08-18 | 李俊伦 | Microgravity experiment instrument |
| EP1500944A1 (en) * | 2003-06-27 | 2005-01-26 | Silvia Bradamante | A bioreactor, in particular for NMR spectroscopy |
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| CN101188069A (en) | 2008-05-28 |
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