CN101144068B - Fast dissolution preparation method for blood culture medium - Google Patents
Fast dissolution preparation method for blood culture medium Download PDFInfo
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- CN101144068B CN101144068B CN 200710045544 CN200710045544A CN101144068B CN 101144068 B CN101144068 B CN 101144068B CN 200710045544 CN200710045544 CN 200710045544 CN 200710045544 A CN200710045544 A CN 200710045544A CN 101144068 B CN101144068 B CN 101144068B
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- culture medium
- preparation
- blood culture
- liquid
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- 239000001963 growth medium Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000009640 blood culture Methods 0.000 title claims abstract description 19
- 238000004090 dissolution Methods 0.000 title claims description 10
- 239000007788 liquid Substances 0.000 claims abstract description 32
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 5
- 239000012530 fluid Substances 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 229920001817 Agar Polymers 0.000 claims abstract description 4
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- 239000008272 agar Substances 0.000 claims abstract description 4
- 230000036512 infertility Effects 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- 239000001509 sodium citrate Substances 0.000 claims abstract description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000012856 packing Methods 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 108010087702 Penicillinase Proteins 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 235000011837 pasties Nutrition 0.000 claims description 3
- 229950009506 penicillinase Drugs 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 235000015278 beef Nutrition 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to the technology field of the biomedicine, in particular to a preparation method for the rapid dissolving of a blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g, beef extract is 3 g, sodium chloride is 5 g, sodium citrate is 3 g, and agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the raw material of the culture medium and agitated into paste, and then 1000ml distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid bottle, and then sterilized in the high pressure; the finished product of the blood culture medium includes the obtaining process that 100 units of penicillase are added in each bottle through an injection syringe or not added, after the sterility test, the blood culture medium is reserved in the room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the filter material, and the operation issimple and convenient.
Description
[technical field]
The present invention relates to field of biomedicine technology, specifically a kind of fast dissolution preparation method for blood culture medium.
[background technology]
Blood culture medium is prepared cumbersome according to a conventional method, and the steps, particularly heating for dissolving such as weighing, Jia Shui, heating for dissolving, filtration, accent pH value, packing, autoclaving are arranged usually, length consuming time, weak effect has muddiness during substratum, impact is to the adjusting of pH value and the observation of bacterial growth situation.
[summary of the invention]
Purpose of the present invention overcomes the deficiencies in the prior art exactly, and the instant compounding process of a kind of blood culture medium that provides.
For achieving the above object, design a kind of fast dissolution preparation method for blood culture medium,
(1) take the culture medium raw material of following component, form instant liquid: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g;
(2) configure instant liquid: the Adlerika of preparation 24.6% is standby as instant liquid 1; The para-amino benzoic acid solution of preparation 0.5% is standby as instant liquid 2;
(3) mix: get the described instant liquid 1 of 20ml and the instant liquid 2 of 10ml, add in culture medium raw material, stir into pasty state, add the distilled water of 1000ml and shake mixing, obtained Clear ﹠ Transparent liquid medium in standing 3-5 minute;
(4) transfer pH value: the pH value of liquid medium is adjusted to 7.4;
(5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle;
(6) autoclaving;
(7) get the blood culture medium finished product; Add penicillinase 100 units or do not add with every bottle of syringe, after sterility test, room storage is standby.
Described instant liquid 1 is that 24.6g sal epsom adding distil water 100ml dissolving is made.
Described instant liquid 2 is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.
Described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.
Described autoclaving was in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
The blood culture medium of described method preparation can be applicable to clinical bacteriology check and blood, marrow is cultivated and checked.
The present invention has compared with prior art removed long-time heating dissolving and filtration step, saves the preparation time, and has saved filtering material, makes easy and simple to handlely, is beneficial to batch production.
[description of drawings]
Fig. 1 is process flow sheet of the present invention.
Appointment Fig. 1 is Figure of abstract.
Referring to Fig. 1,1 for taking culture medium raw material; 2 is preparation of instant liquid; 3 for mixing; 4 for transferring pH value; 5 are packing; 6 is autoclaving; 7 be the blood culture medium finished product.
[specific embodiment]
The present invention is further illustrated below in conjunction with accompanying drawing, and the present invention is still more clearly concerning the people of this professional skill field.
Embodiment:
Take culture medium raw material: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3 grams, nucleic acid 2 grams, agar 0.5 gram, standby.
Preparation of instant liquid:
Instant liquid 1: it is standby that the sal epsom that takes 24.6g adds the dissolving of 100ml distilled water to make 24.6% Adlerika;
Instant liquid 2: the para-amino benzoic acid adding distil water 100ml that takes 0.5g makes 0.5% para-amino benzoic acid solution for standby;
Mix: culture medium raw material is put into large Erlenmeyer flask, then get the instant liquid 1 of 20ml and the instant liquid 2 of 10ml adds in culture medium raw material, after stirring into pasty state, add again the distilled water of 1000ml and shake mixing, standing 3-5 minute, just obtain Clear ﹠ Transparent liquid medium, dissolution rate is fast;
Transfer pH value: with ionocolorimeter, the pH value of liquid medium is adjusted to 7.4;
Packing: being 7.4 liquid medium with pH value is loaded in liquid bottles with the amount of every bottle of 50ml, and with soft rubber ball envelope bottleneck, soft rubber ball is outward again with the aluminium lid press seal;
Autoclaving: in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
At last, add penicillinase 100 units with every bottle of syringe, play the antibiotic effect of neutralization, also can not add, after sterility test, room storage is standby, and these blood culture mediums namely can be used for the use of blood, bone marrow fluid microbial culture.
Claims (5)
1. fast dissolution preparation method for blood culture medium, it is characterized in that: (1) takes the culture medium raw material of following component: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g; (2) preparation of instant liquid: the Adlerika of preparation 24.6% is standby as instant liquid 1; The para-amino benzoic acid solution of preparation 0.5% is standby as instant liquid 2; (3) mix: above-mentioned instant liquid 1 is got 20ml and above-mentioned instant liquid 2 get 10ml and add in culture medium raw material, stir into pasty state, add the distilled water of 1000ml and shake mixing, obtained Clear ﹠ Transparent liquid medium in standing 3-5 minute; (4) transfer pH value: the pH value of liquid medium is adjusted to 7.4; (5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle; (6) autoclaving; (7) get the blood culture medium finished product: add penicillinase 100 units or do not add with every bottle of syringe, after sterility test, room storage is standby.
2. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described instant liquid 1 is that 24.6g sal epsom adding distil water 100ml dissolving is made.
3. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described instant liquid 2 is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.
4. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.
5. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described autoclaving is in high-pressure sterilizing pot, continues sterilization 15 minutes with the pressure of 0.7-1 kilogram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101144068A CN101144068A (en) | 2008-03-19 |
| CN101144068B true CN101144068B (en) | 2013-06-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710045544 Active CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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| Country | Link |
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| CN (1) | CN101144068B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337184B (en) * | 2011-06-09 | 2012-11-07 | 王惠萱 | Solvent, preparation method and application thereof |
| CN102747132A (en) * | 2012-07-23 | 2012-10-24 | 史煜波 | Aerobic bacterial culture solution for machines |
| CN106222236A (en) * | 2016-07-27 | 2016-12-14 | 郑州点石生物技术有限公司 | Microorganism detection reagent and preparation method thereof in blood |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
-
2007
- 2007-09-03 CN CN 200710045544 patent/CN101144068B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
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| Publication number | Publication date |
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| CN101144068A (en) | 2008-03-19 |
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Effective date of registration: 20221228 Address after: 650000 floor 2, building 4, pharmaceutical technology R & D base, intersection of Haiyuan North Road and Keji Road (Yunnan Haobang Investment Co., Ltd.), Kunming, Yunnan Patentee after: Yunnan Dean Medical Laboratory Co.,Ltd. Address before: 650032 Department of clinical laboratory, Kunming general hospital, No. 212 military area, Daguan Road, Yunnan, Kunming Patentee before: Wang Huixuan |