CN101126105A - A method for preparing hypotensive peptide with rice protein isolate - Google Patents
A method for preparing hypotensive peptide with rice protein isolate Download PDFInfo
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- CN101126105A CN101126105A CNA2007100525743A CN200710052574A CN101126105A CN 101126105 A CN101126105 A CN 101126105A CN A2007100525743 A CNA2007100525743 A CN A2007100525743A CN 200710052574 A CN200710052574 A CN 200710052574A CN 101126105 A CN101126105 A CN 101126105A
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Abstract
本发明公开了一种用大米分离蛋白制备降血压肽的方法，其技术要点是：将重量百分比浓度为5－15％(W/W)的大米分离蛋白溶液，用碱性蛋白酶水解，水解的工艺条件为：pH值为8－10、反应温度40－50℃、加酶量1－2％、反应时间120－180min，搅拌酶解。然后经大孔吸附树脂脱盐，树脂为NKA，用40－80％的酒精洗脱。经脱盐后的小分子肽再经浓缩或干燥，制得具有血管紧张素转化酶(ACE)抑制活性的小分子肽。本发明所具有的特点：提供了一种制备降压肽的新方法；工艺科学合理、简单易行；原料来源广泛。The invention discloses a method for preparing blood pressure-lowering peptides from rice protein isolates, the technical points of which are: hydrolyze the rice protein isolate solution with a concentration of 5-15% (W/W) by weight with alkaline protease, and the hydrolyzed The technological conditions are as follows: the pH value is 8-10, the reaction temperature is 40-50° C., the amount of enzyme added is 1-2%, the reaction time is 120-180 minutes, and the enzymolysis is stirred. Then it is desalted through macroporous adsorption resin, the resin is NKA, and eluted with 40-80% alcohol. The desalted small molecular peptides are then concentrated or dried to prepare small molecular peptides with angiotensin converting enzyme (ACE) inhibitory activity. The present invention has the characteristics of providing a new method for preparing the hypotensive peptide; the process is scientific and reasonable, simple and easy; and the sources of raw materials are extensive.
Description
Technical field
The invention belongs to foods processing technique or pharmaceutical technology field, be specifically related to the functional foodstuff processing technology, particularly prepare the method for blood pressure lowering peptide with rice separated protein.
Background technology
ACE inhibitor is with fastest developing speed a kind of in the Altace Ramipril, but the synthetic angiotensin-converting enzyme has untoward reaction, as causing proteinuria and oligoleukocythemia etc., in addition, after drug withdrawal, easily cause " drug withdrawal syndromes ", serious threat patient's life security.
The blood pressure lowering peptide that derives from food proteins can overcome all side effects that occur in the pharmacological agent fully, and it only plays hypotensive effect to the hyperpietic, and the normotensive is not but had hypotensive effect; These peptides are to obtain through enzyme hydrolysis under mild conditions, and safe, it also has other effects such as immunomodulatory simultaneously.Therefore, develop natural decrease blood pressure peptide manufacture and will become important component part in the non-drug therapy.
At present existingly discover that the products of protein behind hydrolysis by novo such as soybean protein, collagen protein and casein have ACE and suppress active, and analyzed by ion-exchange chromatography, high performance liquid chromatography etc. the active component of inhibition is partly arranged.Raw materials that these researchs are selected mostly are animal protein, and separation method or cost are too high, and perhaps ash oontent is higher and influence its activity etc. in the peptide, is not suitable for scale operation.Utilize the hydrolysis by novo rice separated protein to produce blood pressure lowering peptide and yet there are no report both at home and abroad.
In order to overcome the deficiency of aforesaid method, the present invention utilizes the source to crack rice widely or the rice slag is raw material, separation and Extraction is cracked rice or the protein of rice in the slag, utilize the hydrolysis by novo rice separated protein, hydrolysate obtains to have ACE and suppresses active small-molecular peptides after the macroporous adsorbent resin desalination.
Summary of the invention
Of the present inventionly can be achieved through the following technical solutions at present.
With weight percent concentration is the rice separated protein solution of 5-15% (W/W), uses hydrolysis by novo, and the processing condition of hydrolysis are: the pH value is 8-10, temperature of reaction 40-50 ℃, enzyme concentration 0.5-2%, reaction times 120-180min, stirs enzymolysis.Through the macroporous adsorbent resin desalination, the small-molecular peptides after desalination through concentrated or dry, is further made liquid state or solid formulation more then.
Specifically, technology of the present invention comprises 3 key steps, that is: the preparation of rice separated protein → enzymolysis rice separated protein → desalting refinement, wherein desalting refinement selection macroporous adsorbent resin.
Concrete preparation method:
The rice separated protein that separation is obtained is mixed with the solution that concentration is 5-15%, and regulating the pH value is 8-10, and the addition of Sumizyme MP is 0.5-2%.Behind the 40-50 ℃ of hydrolysis 120-180min, centrifugation.After the macroporous adsorbent resin desalting refinement, resin is NKA to supernatant liquor, with the alcohol wash-out of 40-80% through concentrating.Elutriant is sprayed or lyophilize after concentrating recovered alcohol, can obtain to have ACE and suppress active rice blood pressure lowering peptide goods.
The advantage that the present invention has:
1. a kind of novel method for preparing blood pressure lowering peptide is provided
Goods of the present invention have physiological function, and ACE is had extremely strong inhibition activity.The same with the other biological bioactive peptide, have directly, fast, initiatively, the characteristics of complete absorption, particularly effective in cure to cardiovascular disordeies such as hypertension.Finished product of the present invention can be used as functional food, food, foodstuff additive, medicament synergistic agent etc.
2. craft science is reasonable, simple
3. raw material sources are extensive
The selected raw material sources of the present invention are extensive, and aboundresources makes the present invention have stronger suitability.
Embodiment
Embodiment 1
The Sumizyme MP (enzyme activity is 1,500,000 U/g) that adopts Nanning Pang Bo biotechnology company limited to provide, processing condition are: rice separated protein concentration 15%, pH value are 8,50 ℃ of temperature of reaction, enzyme concentration 1%, reaction times 120min.Measure inhibiting rate and the concentration of rice blood pressure lowering peptide goods, calculate IC ACE
50, and ash other indexs of grading see Table 1.
The main physical and chemical index of table 1 rice blood pressure lowering peptide goods
| Project | Index |
| IC 50/ (mg/mL) ash content (in butt)/% soluble nitrogen (in butt)/% peptide (in butt)/% pH (10% aqueous solution) | 0.75 5.5 90.6 79.4 7.2 |
Embodiment 2
The Sumizyme MP (enzyme activity is 600,000 U/g) that adopts Wuxi biotechnology company limited of outstanding energy section to provide, processing condition are: rice separated protein concentration 10%, pH value are 9.5,40 ℃ of temperature of reaction, enzyme concentration 2%, reaction times 180min.Measure inhibiting rate and the concentration of rice blood pressure lowering peptide goods, calculate IC ACE
50, and ash other indexs of grading see Table 2.
The main physical and chemical index of table 2 rice blood pressure lowering peptide goods
| Project | Index |
| IC 50/ (mg/mL) ash content (in butt)/% soluble nitrogen (in butt)/% peptide (in butt)/% pH (10% aqueous solution) | 1.35 6.5 88.2 77.6 6.9 |
Embodiment 3
The Sumizyme MP (enzyme activity is 2.4AU/g) that adopts letter (China) Bioisystech Co., Ltd of Novi to provide, processing condition are: rice separated protein concentration 10%, pH value are 8.5,45 ℃ of temperature of reaction, enzyme concentration 1%, reaction times 150min.Measure inhibiting rate and the concentration of rice blood pressure lowering peptide goods, calculate IC ACE
50, and ash other indexs of grading see Table 3.
The main physical and chemical index of table 3 rice blood pressure lowering peptide goods
| Project | Index |
| IC 50/ (mg/mL) ash content (in butt)/% soluble nitrogen (in butt)/% peptide (in butt)/% pH (10% aqueous solution) | 0.5 3.0 92.4 81.2 7.0 |
Claims (2)
1. method for preparing blood pressure lowering peptide with rice protein, it is characterized in that: be the rice separated protein solution of 5-15% (W/W) with weight percent concentration, use hydrolysis by novo, the processing condition of hydrolysis are: the pH value is 8-10, temperature of reaction 40-50 ℃, enzyme concentration 0.5-2%, reaction times 120-180min, stirs enzymolysis.Then through the macroporous adsorbent resin desalination, make and have ACE and suppress active small-molecular peptides.
2. rice separated protein according to claim 1 prepares the method for blood pressure lowering peptide, it is characterized in that: the small-molecular peptides after desalination through concentrated or dry, is further made liquid state or solid formulation again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100525743A CN101126105A (en) | 2007-06-28 | 2007-06-28 | A method for preparing hypotensive peptide with rice protein isolate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100525743A CN101126105A (en) | 2007-06-28 | 2007-06-28 | A method for preparing hypotensive peptide with rice protein isolate |
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| CN101126105A true CN101126105A (en) | 2008-02-20 |
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| CNA2007100525743A Pending CN101126105A (en) | 2007-06-28 | 2007-06-28 | A method for preparing hypotensive peptide with rice protein isolate |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605030A (en) * | 2012-03-22 | 2012-07-25 | 无锡德冠生物科技有限公司 | Enzymatic extraction method for oat peptide |
| CN102864200A (en) * | 2012-09-17 | 2013-01-09 | 武汉工业学院 | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme |
| CN106615679A (en) * | 2017-02-20 | 2017-05-10 | 和县凤台山煜新畜牧有限公司 | Pig feeding method in which nutritional feed is prepared from soy sauce residues as main ingredient |
| CN106852426A (en) * | 2017-01-02 | 2017-06-16 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of the banana skin beverage with hypotensive activity |
| CN112940079A (en) * | 2021-03-19 | 2021-06-11 | 广州明创生物科技有限公司 | Rice antihypertensive peptide and enzymolysis preparation method thereof |
| WO2021197063A1 (en) * | 2020-03-31 | 2021-10-07 | 华南农业大学 | Oligopeptide having blood pressure reducing effect, and preparation method therefor and application thereof |
-
2007
- 2007-06-28 CN CNA2007100525743A patent/CN101126105A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605030A (en) * | 2012-03-22 | 2012-07-25 | 无锡德冠生物科技有限公司 | Enzymatic extraction method for oat peptide |
| CN102864200A (en) * | 2012-09-17 | 2013-01-09 | 武汉工业学院 | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme |
| CN106852426A (en) * | 2017-01-02 | 2017-06-16 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of the banana skin beverage with hypotensive activity |
| CN106615679A (en) * | 2017-02-20 | 2017-05-10 | 和县凤台山煜新畜牧有限公司 | Pig feeding method in which nutritional feed is prepared from soy sauce residues as main ingredient |
| WO2021197063A1 (en) * | 2020-03-31 | 2021-10-07 | 华南农业大学 | Oligopeptide having blood pressure reducing effect, and preparation method therefor and application thereof |
| CN112940079A (en) * | 2021-03-19 | 2021-06-11 | 广州明创生物科技有限公司 | Rice antihypertensive peptide and enzymolysis preparation method thereof |
| CN112940079B (en) * | 2021-03-19 | 2022-09-09 | 广州明创生物科技有限公司 | Rice antihypertensive peptide and enzymolysis preparation method thereof |
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