CN101118220A - Reagent kit for detecting formaldehyde in flash milk - Google Patents
Reagent kit for detecting formaldehyde in flash milk Download PDFInfo
- Publication number
- CN101118220A CN101118220A CNA2007101538581A CN200710153858A CN101118220A CN 101118220 A CN101118220 A CN 101118220A CN A2007101538581 A CNA2007101538581 A CN A2007101538581A CN 200710153858 A CN200710153858 A CN 200710153858A CN 101118220 A CN101118220 A CN 101118220A
- Authority
- CN
- China
- Prior art keywords
- formaldehyde
- former milk
- kit
- reagent
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 title claims abstract description 122
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 41
- 235000013336 milk Nutrition 0.000 title claims description 48
- 239000008267 milk Substances 0.000 title claims description 48
- 210000004080 milk Anatomy 0.000 title claims description 48
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 75
- 238000006243 chemical reaction Methods 0.000 claims abstract description 15
- QCDYQQDYXPDABM-UHFFFAOYSA-N phloroglucinol Chemical compound OC1=CC(O)=CC(O)=C1 QCDYQQDYXPDABM-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229960001553 phloroglucinol Drugs 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000012360 testing method Methods 0.000 claims description 30
- 238000001514 detection method Methods 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 239000000376 reactant Substances 0.000 claims description 4
- 241000532370 Atla Species 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 10
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- JPYHHZQJCSQRJY-UHFFFAOYSA-N Phloroglucinol Natural products CCC=CCC=CCC=CCC=CCCCCC(=O)C1=C(O)C=C(O)C=C1O JPYHHZQJCSQRJY-UHFFFAOYSA-N 0.000 abstract 1
- 235000020185 raw untreated milk Nutrition 0.000 abstract 1
- 235000019256 formaldehyde Nutrition 0.000 description 30
- 239000000523 sample Substances 0.000 description 18
- 238000005303 weighing Methods 0.000 description 10
- 238000013329 compounding Methods 0.000 description 5
- 230000000007 visual effect Effects 0.000 description 5
- 230000010165 autogamy Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000005562 fading Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- -1 wherein Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to a reagent case, in particular to a reagent case for detecting formaldehyde in raw milk, and belongs to the technical field of chemical components detecting equipment. The formaldehyde quickly detecting reagent case of the present invention is a case which is composed of a device of movable liquid, reacting liquid and a standard color comparing card, wherein the reacting liquid is prepared with the weight proportion of sodium hydroxide, phloroglucinol and water is (10-14) to (0.5-1.5) to 100. The standard color comparing card is a color card with rosiness series, and the lowest detecting limit of the card is 3.8microgramme per ml. Only one step operation of adding reagent is needed in the method, and the color reaction is fast. Only one minute is needed. The measuring condition is relatively loose. The sensitivity is high and the cost is low.
Description
Technical field
The present invention relates to a kind of kit, particularly a kind of kit that detects formaldehyde in the former milk.Belong to chemical analysis checkout equipment technical field.
Background technology
At present in China, the phenomenon that former milk is mingled is prohibited repeatly and is not ended, and more and more serious and general.Because formaldehyde has the corrosion-proof and sterilization effect, under the ordering about of interests, some milk families add the microbiological indicator of formaldehyde when reaching milk collection in former milk, who does not know that formaldehyde is the material than high toxicity, preferentially control on the list formaldehyde at China's toxic chemical and are in second.Formaldehyde is defined as carcinogenic and teratogenesis shape material by the World Health Organization (WHO), is the allergen of generally acknowledging, also is one of potential strong mutagen, and it can destroy biological cell albumen, can cause diseases such as human allergy, enteron aisle irritant reaction, food poisoning.
Fast detecting to formaldehyde in the former milk has corresponding several detection method at present, as acetylacetone method, HNO
3And H
2SO
4Method, FeCl
3Method, FeCl
3And dense H
2SO
4Method, variable color acid system, the luxuriant and rich with fragrance sound of vomiting of iron-neighbour quinoline development process, but, the quality of former milk can not be guaranteed because of being not easy to safe operation, the colour developing shortcoming that phenomenon is not obvious or sensitivity is not high.At present the fast detecting box that is used for detecting formaldehyde in the former milk is specially arranged also on the market in addition, but price comparison is expensive.So at this phenomenon, the inventor proceeds from reality, and considers restriction and security, the convenience consideration from operating of milk collection time, has developed methanal fast-inspecting reagent unit in the former milk voluntarily, its phenomenon is obvious, highly sensitive, and with low cost, easy and safe to operate.
Summary of the invention
The objective of the invention is: develop a kind of kit that detects formaldehyde in the former milk, its phenomenon is obvious, highly sensitive, and with low cost, easy and safe to operate.
Technical scheme of the present invention is as follows:
A kind of kit that detects formaldehyde in the former milk, wherein, formaldehyde repid test kit, the box of forming by device, reactant liquor, test tube, the standard color comparison card of moving liquid; Wherein reactant liquor be by: NaOH, phloroglucin and water weight ratio example are: (10-14): (0.5-1.5): 100 is formulated, and the preferred weight ratio is: 12: 1: 100.Standard color comparison card is the colour atla of pale red series, and this card minimum detectability is 3.8 μ g/ml.Wherein, the device of moving liquid is preferred: syringe, calibrated pipet or liquid-transfering gun that activated charcoal is housed; Standard color comparison card is followed successively by: milky, pale pink, pink colour, light red, orange red, and raise successively for content of formaldehyde.
The mentioned reagent box is to detect according to following steps:
Above-mentioned mix reagent is added in the detected former milk sample, and the part by weight of former milk addition and mix reagent is (4-8): 1, and the preferred weight ratio is: 6: 1;
Whether wait for a period of time, qualitatively judging according to chromogenic reaction has formaldehyde to exist.
1, principle
A reagent (phloroglucin) reacts with formaldehyde under catalyst V reagent (NaOH) catalysis, generates the material that has color of high molecular.
2, reaction reagent
A reagent and V reagent are mixed into mix reagent in certain proportion.The concrete prescription of mix reagent is:
Take by weighing 12g NaOH earlier and be dissolved in the 100mL water, be made into 12% sodium hydroxide solution; And then take by weighing the 1g phloroglucin, be dissolved in 100mL 12% NaOH (NaOH) solution.
3, qualitative detection step:
Directly the former milk sample of 3ml is joined in the test tube, add the compounding of the above-mentioned institute of 0.5ml again and close reagent, not vibration, the chromogenic reaction in 1min bottom the visual observations test tube.
Preferably make a blank test tube during detection, can help paired observation like this.
4, the result judges:
The milk sample of bottom does not develop the color in the test tube, and formaldehyde does not detect;
The milk sample of bottom shows light red in the test tube, contains a small amount of formaldehyde;
The milk sample of bottom shows orange red in the test tube, contains a large amount of formaldehyde.
Annotate: if contain trace, a spot of formaldehyde in the milk sample, light red can occur in the middle bottom of test tube, the time ratio of color fade is shorter, approximately about 1min.If contain relatively large formaldehyde in the milk sample, can deepen in the middle bottom colors of test tube, even whole milk sample all is orange red.
The result of embodiment and discussion
1, mix reagent concentration determines
Found through experiments, the concentration of A reagent changes, and the color of reaction and display is almost constant, and when finding that the A reagent concentration is high, color fading is very fast.When finding that simultaneously the V reagent concentration increases, the color of reaction and display is almost constant, but the solution developing time is too fast, and the time of fading is also fast.So the suitable concentration of A reagent and V reagent has been determined in a large amount of experiment of inventor's process, and is simultaneously also both economical.
2, the mix reagent consumption determines
Found through experiments, when former milk sample solution and mix reagent amount ratio were controlled at 3: 0.5 left and right sides, colour developing relatively was easy to observe.When also find drawing former milk testing sample solution and mix reagent, get liquid measure and need not require very accurately, the effect of chromogenic reaction is the same, and is like this, just just looser to operating personnel's operation requirement.
3, qualitative test standard colors row and detection limit determines
Table 1
| The content of formaldehyde (μ g/ml) in the former milk sample | Color | Qualitative results |
| 0 1.9 3.8 7.6 9.5 19.6 | Milky milky pale pink pink colour light red is orange red | Negative weak positive |
As can be seen from Table 1, detecting of the method is limited to 3.8 μ g/ml.
4, with market in the detection milk sold the quick kit of content of formaldehyde compare
Table 2
| The content of formaldehyde (μ g/ml) in the former milk sample | The autogamy mix reagent | The fast detecting box |
| 0 1.9 3.8 7.6 9.5 19.6 | Milky milky pale pink pink colour light red is orange red | Milky milky pale pink (phenomenon is not obvious) pink colour light red is orange red |
As can be seen from Table 2, the mix reagent of autogamy is higher than the sensitivity of the quick detection reagent of selling on the market.And find that in experimentation the mix reagent fading time of autogamy is slower than kit, more helps operating personnel's observation.
Advantage of the present invention:
This kit only need be prepared a kind of mix reagent, has only a step to add the reagent operation, and chromogenic reaction is fast, only needs about 1 minute, and condition determination is relatively also loose; And highly sensitive, cost is low, in former milk detects, disturb also little, detection limit 3.8 μ g/ml.For big-and-middle-sized dairy products enterprise, be formaldehyde doped method in the former milk of a kind of fast detecting that is highly suitable for popularizing.
Embodiment
The preparation method of kit is a conventional method, constituent wherein such as following embodiment.
Embodiment 1
Take by weighing 12g NaOH earlier and be dissolved in the 100mL water, be made into 12% sodium hydroxide solution; And then take by weighing the 1g phloroglucin, be dissolved in 100mL 12% NaOH (NaOH) solution.
Directly the former milk sample of 3ml is joined in the test tube, add the compounding of the above-mentioned institute of 0.5ml again and close reagent, not vibration, the chromogenic reaction in 1min bottom the visual observations test tube.
Preferably make a blank test tube during detection, can help paired observation like this.
Testing result is as follows:
| The content of formaldehyde (μ g/ml) in the former milk sample | Color | Qualitative results |
| 0 1.8 3.9 7.5 9.3 19.9 | Milky milky pale pink pink colour light red is orange red | Negative weak positive |
Embodiment 2
Take by weighing 14g NaOH earlier and be dissolved in the 100mL water, be made into 14% sodium hydroxide solution; And then take by weighing the 0.5g phloroglucin, be dissolved in 100mL 14% NaOH (NaOH) solution.
Directly the former milk sample of 8ml is joined in the test tube, add the compounding of the above-mentioned institute of 0.5ml again and close reagent, not vibration, the chromogenic reaction in 1min bottom the visual observations test tube.
Preferably make a blank test tube during detection, can help paired observation like this.
Testing result is as follows:
| The content of formaldehyde (μ g/ml) in the former milk sample | Color | Qualitative results |
| 0 1.9 4.5 8.2 9.9 20.9 | Milky milky pale pink pink colour light red is orange red | Negative weak positive |
Embodiment 3
Take by weighing 10g NaOH earlier and be dissolved in the 100mL water, be made into 14% sodium hydroxide solution; And then take by weighing the 1.5g phloroglucin, be dissolved in 100mL 10% NaOH (NaOH) solution.
Directly the former milk sample of 2ml is joined in the test tube, add the compounding of the above-mentioned institute of 0.5ml again and close reagent, not vibration, the chromogenic reaction in 1min bottom the visual observations test tube.
Preferably make a blank test tube during detection, can help paired observation like this.
Testing result is as follows:
| The content of formaldehyde (μ g/ml) in the former milk sample | Color | Qualitative results |
| 0 1.8 4.0 7.6 9.0 18.9 | Milky milky pale pink pink colour light red is orange red | Negative weak positive |
Embodiment 4
Take by weighing 10g NaOH earlier and be dissolved in the 100mL water, be made into 14% sodium hydroxide solution; And then take by weighing the 0.5g phloroglucin, be dissolved in 100mL 10% NaOH (NaOH) solution.
Directly the former milk sample of 2.5ml is joined in the test tube, add the compounding of the above-mentioned institute of 0.5ml again and close reagent, not vibration, the chromogenic reaction in 1min bottom the visual observations test tube.
Preferably make a blank test tube during detection, can help paired observation like this.
Testing result is as follows:
| The content of formaldehyde (μ g/ml) in the former milk sample | Color | Qualitative results |
| 0 1.6 4.1 7.2 9.6 19.9 | Milky milky pale pink pink colour light red is orange red | Negative weak positive |
This kit only need be prepared a kind of mix reagent, has only a step to add the reagent operation, and chromogenic reaction is fast, only needs about 1 minute, and condition determination is relatively also loose; And highly sensitive, cost is low, in former milk detects, disturb also little, detection limit 3.8 μ g/ml.For big-and-middle-sized dairy products enterprise, be formaldehyde doped method in the former milk of a kind of fast detecting that is highly suitable for popularizing.
Claims (6)
1. kit that detects formaldehyde in the former milk is characterized in that: formaldehyde repid test kit, the box of being made up of device, reactant liquor, test tube, the standard color comparison card of moving liquid; Wherein reactant liquor be by: NaOH, phloroglucin and water weight ratio example are: (10-14): (0.5-1.5): 100 is formulated, and standard color comparison card is the colour atla of pale red series, and this card minimum detectability is 3.8 μ g/ml.
2. the kit of formaldehyde in the former milk of detection as claimed in claim 1, it is characterized in that: the device of moving liquid is: syringe, calibrated pipet or liquid-transfering gun that activated charcoal is housed.
3. the kit of formaldehyde in the former milk of detection as claimed in claim 1 is characterized in that: detect according to following steps:
1) above-mentioned mix reagent is added in the detected former milk sample, the part by weight of former milk addition and mix reagent is (4-8): 1;
2) whether wait for a period of time, qualitatively judging according to chromogenic reaction has formaldehyde to exist.
4. the kit of formaldehyde in the former milk of detection as claimed in claim 1, it is characterized in that: NaOH, phloroglucin and water weight ratio example are: 12: 1: 100.
5. the kit of formaldehyde in the former milk of detection as claimed in claim 1, it is characterized in that: the part by weight of former milk addition and mix reagent is: 6: 1.
6. the kit of formaldehyde in the former milk of detection as claimed in claim 1, it is characterized in that: described standard color comparison card is followed successively by: milky, pale pink, pink colour, light red, orange red.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101538581A CN100547391C (en) | 2007-09-14 | 2007-09-14 | A test kit for detecting formaldehyde in raw milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101538581A CN100547391C (en) | 2007-09-14 | 2007-09-14 | A test kit for detecting formaldehyde in raw milk |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101118220A true CN101118220A (en) | 2008-02-06 |
| CN100547391C CN100547391C (en) | 2009-10-07 |
Family
ID=39054421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007101538581A Active CN100547391C (en) | 2007-09-14 | 2007-09-14 | A test kit for detecting formaldehyde in raw milk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100547391C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010127765A1 (en) * | 2009-05-05 | 2010-11-11 | Merck Patent Gmbh | Agent and method for determining aldehyde content |
| CN106153611A (en) * | 2015-04-24 | 2016-11-23 | 广东华润涂料有限公司 | For quickly detection polymer emulsion or comprise described polymer emulsion preparation in the method for formaldehyde |
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106546582A (en) * | 2016-11-04 | 2017-03-29 | 东莞市食品药品检验所 | The quick detection reagent and method of hexamethylenamine in food |
-
2007
- 2007-09-14 CN CNB2007101538581A patent/CN100547391C/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010127765A1 (en) * | 2009-05-05 | 2010-11-11 | Merck Patent Gmbh | Agent and method for determining aldehyde content |
| CN106153611A (en) * | 2015-04-24 | 2016-11-23 | 广东华润涂料有限公司 | For quickly detection polymer emulsion or comprise described polymer emulsion preparation in the method for formaldehyde |
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
| CN110118770B (en) * | 2019-04-19 | 2022-06-21 | 广州安诺科技股份有限公司 | Reagent and kit for detecting sodium formaldehyde sulfoxylate in food and production process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100547391C (en) | 2009-10-07 |
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